Pub Date : 2024-06-27DOI: 10.1016/j.biortech.2024.131051
Zijing Guo , Fangshu Qu , Jie Wang , Mingyue Geng , Shanshan Gao , Jiayu Tian
Strengthening the direct interspecies electron transfer (DIET) is an effective strategy to improve the performance of anaerobic digestion (AD) process. In this study, the polyaniline functionated activated carbon (AC-PANi) was prepared by chemical oxidative polymerization. This material possessed pseudo-capacitance properties as well as excellent charge transfer capability. The experimental results demonstrated that the incorporation of AC-PANi in AD process could efficiently increase the chemical oxygen demand (COD) removal (18.6 %) and daily methane production rate (35.3 %). The AC-PANi can also act as an extracellular acceptor to promote the synthesis of adenosine triphosphate (ATP) and secretion of extracellular enzymes as well as cytochrome C (Cyt-C). The content of coenzyme F420 on methanogens was also shown to be increased by 60.9 % with the addition of AC-PANi in AD reactor. Overall, this work provides an easy but feasible way to enhance AD performance by promoting DIET between acetate-producing bacteria and methanogens.
加强种间直接电子传递(DIET)是提高厌氧消化(AD)工艺性能的有效策略。本研究采用化学氧化聚合法制备了聚苯胺功能活性炭(AC-PANi)。这种材料具有伪电容特性和出色的电荷转移能力。实验结果表明,在厌氧消化(AD)工艺中加入 AC-PANi 能有效提高化学需氧量(COD)去除率(18.6%)和甲烷日产量(35.3%)。AC-PANi 还可作为细胞外接受体,促进三磷酸腺苷(ATP)的合成和细胞外酶以及细胞色素(Cyt-C)的分泌。在厌氧消化反应器中添加 AC-PANi 后,甲烷菌体内辅酶 F 的含量也增加了 60.9%。总之,这项工作提供了一种简便可行的方法,通过促进醋酸生产菌和甲烷菌之间的DIET来提高厌氧消化反应的性能。
{"title":"Enhancing electron transfer in anaerobic process by supercapacitor materials: Polyaniline functionated activated carbon","authors":"Zijing Guo , Fangshu Qu , Jie Wang , Mingyue Geng , Shanshan Gao , Jiayu Tian","doi":"10.1016/j.biortech.2024.131051","DOIUrl":"10.1016/j.biortech.2024.131051","url":null,"abstract":"<div><p>Strengthening the direct interspecies electron transfer (DIET) is an effective strategy to improve the performance of anaerobic digestion (AD) process. In this study, the polyaniline functionated activated carbon (AC-PANi) was prepared by chemical oxidative polymerization. This material possessed pseudo-capacitance properties as well as excellent charge transfer capability. The experimental results demonstrated that the incorporation of AC-PANi in AD process could efficiently increase the chemical oxygen demand (COD) removal (18.6 %) and daily methane production rate (35.3 %). The AC-PANi can also act as an extracellular acceptor to promote the synthesis of adenosine triphosphate (ATP) and secretion of extracellular enzymes as well as cytochrome <em>C</em> (Cyt-C). The content of coenzyme F<sub>420</sub> on methanogens was also shown to be increased by 60.9 % with the addition of AC-PANi in AD reactor. Overall, this work provides an easy but feasible way to enhance AD performance by promoting DIET between acetate-producing bacteria and methanogens.</p></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":null,"pages":null},"PeriodicalIF":9.7,"publicationDate":"2024-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141463318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-27DOI: 10.1016/j.biortech.2024.131053
Ji-Wen Yao , Xiao-Yan Huang , Yen-Han Lin , Chen-Guang Liu , Feng-Wu Bai
Lignocellulose presents a promising alternative to fossil fuels. Monitoring the mass and size changes of lignocellulosic particles without disrupting the process can assist in adjusting pretreatment and enzymatic hydrolysis, where conventional sieving methods fall short. A method utilizing focused beam reflectance measurement (FBRM) was developed to establish mathematical correlations between FBRM chord information (chord length and count) and particle characteristics (weight and size) quantified through sieving. Results indicate particle size exhibits a linear correlation with the square weighted median chord length (Lsqr) with R2 at 0.93. Further, real-time bulk particle mass can be predicted using Lsqr and chord count (R2 0.98). These correlations are applicable in range 53 μm to 358.5 μm. Real-time monitoring of enzymatic hydrolysis of corn stalks has demonstrated the practical applicability of FBRM. This study introduces a novel approach for online characterization of lignocellulosic particles, thereby enhancing lignocellulosic biorefineries.
{"title":"Online monitoring lignocellulosic particles by focus beam reflectance measurement for efficient bioprocessing","authors":"Ji-Wen Yao , Xiao-Yan Huang , Yen-Han Lin , Chen-Guang Liu , Feng-Wu Bai","doi":"10.1016/j.biortech.2024.131053","DOIUrl":"10.1016/j.biortech.2024.131053","url":null,"abstract":"<div><p>Lignocellulose presents a promising alternative to fossil fuels. Monitoring the mass and size changes of lignocellulosic particles without disrupting the process can assist in adjusting pretreatment and enzymatic hydrolysis, where conventional sieving methods fall short. A method utilizing focused beam reflectance measurement (FBRM) was developed to establish mathematical correlations between FBRM chord information (chord length and count) and particle characteristics (weight and size) quantified through sieving. Results indicate particle size exhibits a linear correlation with the square weighted median chord length (L<sub>sqr</sub>) with R<sup>2</sup> at 0.93. Further, real-time bulk particle mass can be predicted using L<sub>sqr</sub> and chord count (R<sup>2</sup> 0.98). These correlations are applicable in range 53 μm to 358.5 μm. Real-time monitoring of enzymatic hydrolysis of corn stalks has demonstrated the practical applicability of FBRM. This study introduces a novel approach for online characterization of lignocellulosic particles, thereby enhancing lignocellulosic biorefineries.</p></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":null,"pages":null},"PeriodicalIF":9.7,"publicationDate":"2024-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141463333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-27DOI: 10.1016/j.biortech.2024.131039
Daniel Nframah Ampong , Wang Lin , Felipe M. de Souza , Vikram Kishore Bharti , Frank Ofori Agyemang , Anthony Andrews , Kwadwo Mensah-Darkwa , Alisha Dhakal , Sanjay R. Mishra , Felio Perez , Ram K. Gupta
In this work, carbonization and subsequent activation procedures were adopted to synthesize waste shea butter shells into oxygen-rich interconnected porous activated carbon (SAC_x, x is the mass ratio of KOH used for activation). The SAC_1.5 electrode material showed outstanding electrochemical performance with high specific capacitance (286.6 F/g) and improved rate capability, owing to various synergistic effects originating from a high specific surface area (1233.5 m2/g) and O-rich content. The SAC_1.5-based symmetric device delivered an impressive specific capacitance of 91.6 F/g with a high energy density of 12.7 Wh/kg at 0.5 A/g. The device recorded 99.9 % capacitance retention after 10,000 charge-discharge cycles. The symmetric supercapacitor device successfully lit an LED bulb for more than 1 h, signifying the potential of bio-waste as an efficient carbon precursor for electrode material in practical supercapacitors. This work offers an efficient, affordable, and environmentally friendly strategy for potential renewable energy storage devices.
{"title":"Utilization of shea butter waste-derived hierarchical activated carbon for high-performance supercapacitor applications","authors":"Daniel Nframah Ampong , Wang Lin , Felipe M. de Souza , Vikram Kishore Bharti , Frank Ofori Agyemang , Anthony Andrews , Kwadwo Mensah-Darkwa , Alisha Dhakal , Sanjay R. Mishra , Felio Perez , Ram K. Gupta","doi":"10.1016/j.biortech.2024.131039","DOIUrl":"10.1016/j.biortech.2024.131039","url":null,"abstract":"<div><p>In this work, carbonization and subsequent activation procedures were adopted to synthesize waste shea butter shells into oxygen-rich interconnected porous activated carbon (SAC_<em>x,</em> x is the mass ratio of KOH used for activation). The SAC_1.5 electrode material showed outstanding electrochemical performance with high specific capacitance (286.6 F/g) and improved rate capability, owing to various synergistic effects originating from a high specific surface area (1233.5 m<sup>2</sup>/g) and O-rich content. The SAC_1.5-based symmetric device delivered an impressive specific capacitance of 91.6 F/g with a high energy density of 12.7 Wh/kg at 0.5 A/g. The device recorded 99.9 % capacitance retention after 10,000 charge-discharge cycles. The symmetric supercapacitor device successfully lit an LED bulb for more than 1 h, signifying the potential of bio-waste as an efficient carbon precursor for electrode material in practical supercapacitors. This work offers an efficient, affordable, and environmentally friendly strategy for potential renewable energy storage devices.</p></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":null,"pages":null},"PeriodicalIF":9.7,"publicationDate":"2024-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141462161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-27DOI: 10.1016/j.biortech.2024.131052
Cheng-Wei Chung, I-Son Ng
Cyanobacteria hold promise for simultaneous carbon capture and chemicals production, but the regulation and effect of nitrogen (N) and phosphorus (P) remains unclear. This study investigates major productions of glycogen, protein, and C-phycocyanin (C-PC) in Cyanobacterium aponinum PCC10605 under different N/P levels, alongside changes in light and CO2. Increasing nitrate (NO3–) from 2 to 6 mM resulted in a 9.7-fold increase in C-PC and reduced glycogen to 8.9 %. On the other hand, elevating phosphorus from 0.1 to 2 mM under limited nitrogen enhanced biomass and glycogen through the upregulation of carbonic anhydrase, ADP-glucose pyrophosphorylase, and glycogen phosphorylase. Changes in phosphorus levels and CO2 inlet concentrations affected metabolites accumulation and carbon capture efficiency, leading to the best condition of 76 % uptake capacity in direct air capture (DAC). All findings underscore the trade-off between glycogen and protein, representing the importance of N/P levels in nutrient modulation of PCC10605.
{"title":"Tailoring nitrogen and phosphorus levels for tunable glycogen and protein production in halophilic Cyanobacterium aponinum PCC10605","authors":"Cheng-Wei Chung, I-Son Ng","doi":"10.1016/j.biortech.2024.131052","DOIUrl":"10.1016/j.biortech.2024.131052","url":null,"abstract":"<div><p>Cyanobacteria hold promise for simultaneous carbon capture and chemicals production, but the regulation and effect of nitrogen (N) and phosphorus (P) remains unclear. This study investigates major productions of glycogen, protein, and C-phycocyanin (C-PC) in <em>Cyanobacterium aponinum</em> PCC10605 under different N/P levels, alongside changes in light and CO<sub>2</sub>. Increasing nitrate (NO<sub>3</sub><sup>–</sup>) from 2 to 6 mM resulted in a 9.7-fold increase in C-PC and reduced glycogen to 8.9 %. On the other hand, elevating phosphorus from 0.1 to 2 mM under limited nitrogen enhanced biomass and glycogen through the upregulation of carbonic anhydrase, ADP-glucose pyrophosphorylase, and glycogen phosphorylase. Changes in phosphorus levels and CO<sub>2</sub> inlet concentrations affected metabolites accumulation and carbon capture efficiency, leading to the best condition of 76 % uptake capacity in direct air capture (DAC). All findings underscore the trade-off between glycogen and protein, representing the importance of N/P levels in nutrient modulation of PCC10605.</p></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":null,"pages":null},"PeriodicalIF":9.7,"publicationDate":"2024-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141463792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-27DOI: 10.1016/j.biortech.2024.131054
Yun Zhou , Xiaocai Cui , Beibei Wu , Ziqi Wang , Ying Liu , Tian Ren , Siqing Xia , Bruce E. Rittmann
Microalgae extracellular polymeric substances (EPS) are complex high-molecular-weight polymers and the physicochemical properties of EPS strongly affect the core features of microalgae cultivation and resource utilization. Revealing the key roles of EPS in microalgae life-cycle processes in an interesting and novelty topic to achieve energy-efficient practical application of microalgae. This review found that EPS showed positive effect in non-gas uptake, extracellular electron transfer, toxicity resistance and heterotrophic symbiosis, but negative impact in gas transfer and light utilization during microalgae cultivation. For biomass harvesting, EPS favored biomass flocculation and large-size cell self-flocculation, but unfavored small size microalgae self-flocculation, membrane filtration, charge neutralization and biomass dewatering. During bioproducts extraction, EPS exhibited positive impact in extractant uptake, but the opposite effect in cellular membrane permeability and cell rupture. Future research on microalgal EPS were also identified, which offer suggestions for comprehensive understanding of microalgal EPS roles in various scenarios.
{"title":"Microalgal extracellular polymeric substances (EPS) and their roles in cultivation, biomass harvesting, and bioproducts extraction","authors":"Yun Zhou , Xiaocai Cui , Beibei Wu , Ziqi Wang , Ying Liu , Tian Ren , Siqing Xia , Bruce E. Rittmann","doi":"10.1016/j.biortech.2024.131054","DOIUrl":"10.1016/j.biortech.2024.131054","url":null,"abstract":"<div><p>Microalgae extracellular polymeric substances (EPS) are complex high-molecular-weight polymers and the physicochemical properties of EPS strongly affect the core features of microalgae cultivation and resource utilization. Revealing the key roles of EPS in microalgae life-cycle processes in an interesting and novelty topic to achieve energy-efficient practical application of microalgae. This review found that EPS showed positive effect in non-gas uptake, extracellular electron transfer, toxicity resistance and heterotrophic symbiosis, but negative impact in gas transfer and light utilization during microalgae cultivation. For biomass harvesting, EPS favored biomass flocculation and large-size cell self-flocculation, but unfavored small size microalgae self-flocculation, membrane filtration, charge neutralization and biomass dewatering. During bioproducts extraction, EPS exhibited positive impact in extractant uptake, but the opposite effect in cellular membrane permeability and cell rupture. Future research on microalgal EPS were also identified, which offer suggestions for comprehensive understanding of microalgal EPS roles in various scenarios.</p></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":null,"pages":null},"PeriodicalIF":9.7,"publicationDate":"2024-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141464787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-26DOI: 10.1016/j.biortech.2024.131040
Yuan Yao , Aidin Panahi , Martin Schiemann , Yiannis A. Levendis
This laboratory study reports results on the group particle combustion of pulverized bituminous coal and various types of torrefied biomass. Combustion of particle streams in a drop tube furnace in air was concurrently monitored by a spectrometer and an electronic camera to obtain spectral emissivities and temperatures. As particle number density (PND) increased, biomass particles became more prone than coal to group combustion. Spectral emissivities increased with increasing PND from 0.2 to 0.4 for coal and from 0.1 to 0.3 for biomass, in the wavelength domain of λ = 600–1000 nm. Emissivities changed little with wavelength, giving credence to the gray body assumption. Particle cloud temperatures were in the range of 1650–1900 K, depending on PND, type of fuel, and location in the cloud; temperatures decreased with increasing PND. The radiative heat of the particle laden flames was predominantly attributed to burning chars in the flames and it increased with increasing PND.
{"title":"On the temperature and emissivity of torrefied biomass and coal in group particle combustion","authors":"Yuan Yao , Aidin Panahi , Martin Schiemann , Yiannis A. Levendis","doi":"10.1016/j.biortech.2024.131040","DOIUrl":"10.1016/j.biortech.2024.131040","url":null,"abstract":"<div><p>This laboratory study reports results on the group particle combustion of pulverized bituminous coal and various types of torrefied biomass. Combustion of particle streams in a drop tube furnace in air was concurrently monitored by a spectrometer and an electronic camera to obtain spectral emissivities and temperatures. As particle number density (PND) increased, biomass particles became more prone than coal to group combustion. Spectral emissivities increased with increasing PND from 0.2 to 0.4 for coal and from 0.1 to 0.3 for biomass, in the wavelength domain of λ = 600–1000 nm. Emissivities changed little with wavelength, giving credence to the gray body assumption. Particle cloud temperatures were in the range of 1650–1900 K, depending on PND, type of fuel, and location in the cloud; temperatures decreased with increasing PND. The radiative heat of the particle laden flames was predominantly attributed to burning chars in the flames and it increased with increasing PND.</p></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":null,"pages":null},"PeriodicalIF":9.7,"publicationDate":"2024-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0960852424007442/pdfft?md5=5ad94bd6c4039a44020b219692ba97ce&pid=1-s2.0-S0960852424007442-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141463206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-26DOI: 10.1016/j.biortech.2024.131050
Mi Tang , Jiajia You , Tianjin Yang , Qisheng Sun , Shuran Jiang , Meijuan Xu , Xuewei Pan , Zhiming Rao
Aromatic amino acids (AAA) and derived compounds have enormous commercial value with extensive applications in the food, chemical and pharmaceutical fields. Microbial production of AAA and derived compounds is a promising prospect for its environmental friendliness and sustainability. However, low yield and production efficiency remain major challenges for realizing industrial production. With the advancement of synthetic biology, microbial production of AAA and derived compounds has been significantly facilitated. In this review, a comprehensive overview on the current progresses, challenges and corresponding solutions for AAA and derived compounds biosynthesis is provided. The most cutting-edge developments of synthetic biology technology in AAA and derived compounds biosynthesis, including CRISPR-based system, genetically encoded biosensors and synthetic genetic circuits, were highlighted. Finally, future prospects of modern strategies conducive to the biosynthesis of AAA and derived compounds are discussed. This review offers guidance on constructing microbial cell factory for aromatic compound using synthetic biology technology.
{"title":"Application of modern synthetic biology technology in aromatic amino acids and derived compounds biosynthesis","authors":"Mi Tang , Jiajia You , Tianjin Yang , Qisheng Sun , Shuran Jiang , Meijuan Xu , Xuewei Pan , Zhiming Rao","doi":"10.1016/j.biortech.2024.131050","DOIUrl":"10.1016/j.biortech.2024.131050","url":null,"abstract":"<div><p>Aromatic amino acids (AAA) and derived compounds have enormous commercial value with extensive applications in the food, chemical and pharmaceutical fields. Microbial production of AAA and derived compounds is a promising prospect for its environmental friendliness and sustainability. However, low yield and production efficiency remain major challenges for realizing industrial production. With the advancement of synthetic biology, microbial production of AAA and derived compounds has been significantly facilitated. In this review, a comprehensive overview on the current progresses, challenges and corresponding solutions for AAA and derived compounds biosynthesis is provided. The most cutting-edge developments of synthetic biology technology in AAA and derived compounds biosynthesis, including CRISPR-based system, genetically encoded biosensors and synthetic genetic circuits, were highlighted. Finally, future prospects of modern strategies conducive to the biosynthesis of AAA and derived compounds are discussed. This review offers guidance on constructing microbial cell factory for aromatic compound using synthetic biology technology.</p></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":null,"pages":null},"PeriodicalIF":9.7,"publicationDate":"2024-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141463148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Visible light-driven intimately coupled photocatalysis and biodegradation (VDICPB) is an efficient technology for removing recalcitrant contaminants, but the degradation pathway on 17β-estradiol 3-Sulfate (E2-3S) is still not clear. In this study, VDICPB based on N-doped TiO2 as a photocatalyst was established to investigate the removal and transformation of E2-3S in synthetic wastewater. VDICPB showed a satisfactory removal efficiency of 97.8 ± 0.4 %, which was much higher than that of independent photocatalysis (84.0 ± 2.2 %) or biodegradation system (71.4 ± 1.8 %). Steroid C/D-rings of E2-3S was broken in VDICPB since the transformation process reached terminal central pathway. Primary metabolites did not accumulate in VDICPB, resulting in a low expression of functional genes. E2-3S was mainly removed by cooperative interaction of photocatalysis and co-metabolism of biofilm. Photocatalysis led to deconjugation and microbes acted to mineralization. This study provides technical reference and theoretical support for the removal of new pollutants.
{"title":"Degradation of conjugated estrogen in visible light-driven intimately coupled photocatalysis and biodegradation system","authors":"Xiangwei Ding , Qingmiao Yu , Hongqiang Ren , Jinju Geng","doi":"10.1016/j.biortech.2024.131045","DOIUrl":"10.1016/j.biortech.2024.131045","url":null,"abstract":"<div><p>Visible light-driven intimately coupled photocatalysis and biodegradation (VDICPB) is an efficient technology for removing recalcitrant contaminants, but the degradation pathway on 17β-estradiol 3-Sulfate (E2-3S) is still not clear. In this study, VDICPB based on N-doped TiO<sub>2</sub> as a photocatalyst was established to investigate the removal and transformation of E2-3S in synthetic wastewater. VDICPB showed a satisfactory removal efficiency of 97.8 ± 0.4 %, which was much higher than that of independent photocatalysis (84.0 ± 2.2 %) or biodegradation system (71.4 ± 1.8 %). Steroid C/D-rings of E2-3S was broken in VDICPB since the transformation process reached terminal central pathway. Primary metabolites did not accumulate in VDICPB, resulting in a low expression of functional genes. E2-3S was mainly removed by cooperative interaction of photocatalysis and co-metabolism of biofilm. Photocatalysis led to deconjugation and microbes acted to mineralization. This study provides technical reference and theoretical support for the removal of new pollutants.</p></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":null,"pages":null},"PeriodicalIF":9.7,"publicationDate":"2024-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141462153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-26DOI: 10.1016/j.biortech.2024.131047
Zimin Hu , Jingjing Li , Jun Qian , Jin Liu , Wenguang Zhou
This study investigated the efficacy of the rotating algal biofilm (RAB) for treating soy sauce wastewater (SW) and its related treatment mechanisms. The RAB system demonstrated superior nutrient removal (chemical oxygen demand, ammonium nitrogen, total nitrogen, and phosphorus for 92 %, 94 %, 91 %, and 82 %, respectively) and biofilm productivity (14 g m−2 d–1) at optimized 5-day harvest time and 2-day hydraulic retention time. This was mainly attributed to the synergistic interactions within the algae-fungi (Apiotrichum)-bacteria (Acinetobacter and Rhizobia) consortium, which effectively assimilated certain extracellular polymeric substances into biomass to enhance algal biofilm growth. Increased algal productivity notably improved protein and essential amino acid contents in the biomass, suggesting a potential for animal feed applications. This study not only demonstrates a sustainable approach for managing SW but also provides insight into the nutrient removal and biomass conversion, offering a viable strategy for large-scale applications in nutrient recovery and wastewater treatment.
本研究调查了旋转藻类生物膜(RAB)处理酱油废水(SW)的功效及其相关处理机制。在优化的 5 天收获时间和 2 天水力停留时间下,旋转藻类生物膜系统表现出卓越的营养物去除率(化学需氧量、氨氮、总氮和磷的去除率分别为 92%、94%、91% 和 82%)和生物膜生产力(14 g m d)。这主要归功于藻类-真菌()-细菌(和)联合体内部的协同作用,它们有效地将某些胞外聚合物物质同化为生物质,从而促进了藻类生物膜的生长。藻类生产率的提高显著改善了生物质中蛋白质和必需氨基酸的含量,这表明其具有应用于动物饲料的潜力。这项研究不仅展示了一种可持续的 SW 管理方法,还深入探讨了营养物质的去除和生物质的转化,为营养物质回收和废水处理的大规模应用提供了一种可行的策略。
{"title":"Efficacy and mechanisms of rotating algal biofilm system in remediation of soy sauce wastewater","authors":"Zimin Hu , Jingjing Li , Jun Qian , Jin Liu , Wenguang Zhou","doi":"10.1016/j.biortech.2024.131047","DOIUrl":"10.1016/j.biortech.2024.131047","url":null,"abstract":"<div><p>This study investigated the efficacy of the rotating algal biofilm (RAB) for treating soy sauce wastewater (SW) and its related treatment mechanisms. The RAB system demonstrated superior nutrient removal (chemical oxygen demand, ammonium nitrogen, total nitrogen, and phosphorus for 92 %, 94 %, 91 %, and 82 %, respectively) and biofilm productivity (14 g m<sup>−2</sup> d<sup>–1</sup>) at optimized 5-day harvest time and 2-day hydraulic retention time. This was mainly attributed to the synergistic interactions within the algae-fungi (<em>Apiotrichum</em>)-bacteria (<em>Acinetobacter</em> and <em>Rhizobia</em>) consortium, which effectively assimilated certain extracellular polymeric substances into biomass to enhance algal biofilm growth. Increased algal productivity notably improved protein and essential amino acid contents in the biomass, suggesting a potential for animal feed applications. This study not only demonstrates a sustainable approach for managing SW but also provides insight into the nutrient removal and biomass conversion, offering a viable strategy for large-scale applications in nutrient recovery and wastewater treatment.</p></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":null,"pages":null},"PeriodicalIF":9.7,"publicationDate":"2024-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141462162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-26DOI: 10.1016/j.biortech.2024.131049
Lichun Guo , Bingwen Xi , Liushen Lu
Increasing evidence shows that microbial synthesis plays an important role in producing high value-added products. However, microbial monoculture generally hampers metabolites production and limits scalability due to the increased metabolic burden on the host strain. In contrast, co-culture is a more flexible approach to improve the environmental adaptability and reduce the overall metabolic burden. The well-defined co-culturing microbial consortia can tap their metabolic potential to obtain yet-to-be discovered and pre-existing metabolites. This review focuses on the use of a co-culture strategy and its underlying mechanisms to enhance the production of products. Notably, the significance of comprehending the microbial interactions, diverse communication modes, genetic information, and modular co-culture involved in co-culture systems were highlighted. Furthermore, it addresses the current challenges and outlines potential future directions for microbial co-culture. This review provides better understanding the diversity and complexity of the interesting interaction and communication to advance the development of co-culture techniques.
{"title":"Strategies to enhance production of metabolites in microbial co-culture systems","authors":"Lichun Guo , Bingwen Xi , Liushen Lu","doi":"10.1016/j.biortech.2024.131049","DOIUrl":"10.1016/j.biortech.2024.131049","url":null,"abstract":"<div><p>Increasing evidence shows that microbial synthesis plays an important role in producing high value-added products. However, microbial monoculture generally hampers metabolites production and limits scalability due to the increased metabolic burden on the host strain. In contrast, co-culture is a more flexible approach to improve the environmental adaptability and reduce the overall metabolic burden. The well-defined co-culturing microbial consortia can tap their metabolic potential to obtain yet-to-be discovered and pre-existing metabolites. This review focuses on the use of a co-culture strategy and its underlying mechanisms to enhance the production of products. Notably, the significance of comprehending the microbial interactions, diverse communication modes, genetic information, and modular co-culture involved in co-culture systems were highlighted. Furthermore, it addresses the current challenges and outlines potential future directions for microbial co-culture. This review provides better understanding the diversity and complexity of the interesting interaction and communication to advance the development of co-culture techniques.</p></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":null,"pages":null},"PeriodicalIF":9.7,"publicationDate":"2024-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141463232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}