Pub Date : 2026-02-04DOI: 10.1016/j.biortech.2026.134149
Carlos Eduardo Lach , Daniele Damasceno Silveira , Tiago José Belli , Flávio Rubens Lapolli , María Ángeles Lobo-Recio
This study investigates a hybrid bioelectrochemical system that integrates an anodic biofilm (ABF) with a cathodic bio-electro-Fenton (BEF) process for the treatment of azo–dye–containing wastewater. Three operational strategies were evaluated by varying the RBV-5R/acetate ratio and hydraulic retention time (HRT). Under optimal conditions (20 mg L−1 RBV-5R, 0.25 g L−1 acetate, 6 h/12 h ABF/BEF), the system achieved a power density of 73.3 mW m−2 and in situ H2O2 generation of 12.3 ± 0.2 mg L−1, resulting in high removals of color (99.8%), COD (79.6%,), and a marked reduction in phytotoxicity after pH neutralization. Unlike conventional MFC–BEF configurations, this work demonstrates a redox-sequential, self-powered ABF + BEF architecture in which the anodic biofilm serves as an active pretreatment stage prior to oxidative polishing. These results highlight the potential of this integrated platform as a sustainable strategy for advanced wastewater treatment of azo dyes.
本研究研究了一种混合生物电化学系统，该系统将阳极生物膜（ABF）与阴极生物电- fenton （BEF）工艺相结合，用于处理含偶氮染料的废水。通过改变RBV-5R/乙酸酯比和水力停留时间（HRT），对三种操作策略进行了评估。在最佳条件下（20 mg L - 1 RBV-5R， 0.25 g L - 1醋酸盐，6 h/12 h ABF/BEF），该系统的功率密度为73.3 mW m - 2，原位H2O2生成为12.3±0.2 mg L - 1，具有较高的去除率（99.8%），COD(79.6%)，并且pH中和后的植物毒性显著降低。与传统的MFC-BEF结构不同，这项研究展示了一种氧化还原顺序的、自供电的ABF + BEF结构，其中阳极生物膜在氧化抛光之前起到了积极的预处理作用。这些结果突出了该综合平台作为偶氮染料高级废水处理可持续战略的潜力。
{"title":"Bioelectrochemical hybrid system integrating anodic biofilm and cathodic Fenton into a microbial fuel cell for multifunctional treatment of azo dye wastewater","authors":"Carlos Eduardo Lach , Daniele Damasceno Silveira , Tiago José Belli , Flávio Rubens Lapolli , María Ángeles Lobo-Recio","doi":"10.1016/j.biortech.2026.134149","DOIUrl":"10.1016/j.biortech.2026.134149","url":null,"abstract":"<div><div>This study investigates a hybrid bioelectrochemical system that integrates an anodic biofilm (ABF) with a cathodic bio-electro-Fenton (BEF) process for the treatment of azo–dye–containing wastewater. Three operational strategies were evaluated by varying the RBV-5R/acetate ratio and hydraulic retention time (HRT). Under optimal conditions (20 mg L<sup>−1</sup> RBV-5R, 0.25 g L<sup>−1</sup> acetate, 6 h/12 h ABF/BEF), the system achieved a power density of 73.3 mW m<sup>−2</sup> and in situ H<sub>2</sub>O<sub>2</sub> generation of 12.3 ± 0.2 mg L<sup>−1</sup>, resulting in high removals of color (99.8%), COD (79.6%,), and a marked reduction in phytotoxicity after pH neutralization. Unlike conventional MFC–BEF configurations, this work demonstrates a redox-sequential, self-powered ABF + BEF architecture in which the anodic biofilm serves as an active pretreatment stage prior to oxidative polishing. These results highlight the potential of this integrated platform as a sustainable strategy for advanced wastewater treatment of azo dyes.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"446 ","pages":"Article 134149"},"PeriodicalIF":9.0,"publicationDate":"2026-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146122630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-02-04DOI: 10.1016/j.biortech.2026.134148
Fatima Anjum, Davide Mattia, Ehsan Nourafkan, Ming Xie, Gary J Lye, Hannah S Leese
The growing global demand for oils and lipids, alongside the environmental impact of traditional oil crop cultivation, has generated significant interest in fermentation (or specifically precision fermentation) of oleaginous yeasts to produce food-grade bio-oils. While research is growing in upstream processing and innovation, downstream processing, which includes cell harvesting, disruption, oil extraction, and purification, remains underexplored. Current downstream methods are largely chemical based due to their high yields, established protocols, and operational simplicity. However, these current methods raise concerns regarding food safety and environmental sustainability. This review provides a comprehensive overview of these challenges across the various stages of downstream processing and examines their impact on process efficiency, sustainability, and scalability. The review also identifies key research gaps and proposes future research directions to advance the field and takes a whole-system approach to the sustainable production of food grade yeast oils. Furthermore, the review proposes novel research paths by analysing and drawing inspiration from oil recovery in biorefinery research. Developing efficient downstream processes for yeast-derived oils presents several challenges, including complex bio-separation steps, limited sustainable alternatives, high capital and energy requirements, scalability issues, and a lack of integrated process understanding. There are promising strategies from biorefinery research, such as innovations in solvents, novel adsorption techniques, advances in membrane technology and insitu conversion to finished products, which could be adapted (considering appropriate regulatory frameworks) to process food-grade yeast oils. The insights within this review aim to support the development of scalable, safe, and sustainable downstream processes to meet the rising demand for alternative oils.
{"title":"Reimagining oil recovery: Sustainable downstream processing of oleaginous yeasts for food applications.","authors":"Fatima Anjum, Davide Mattia, Ehsan Nourafkan, Ming Xie, Gary J Lye, Hannah S Leese","doi":"10.1016/j.biortech.2026.134148","DOIUrl":"https://doi.org/10.1016/j.biortech.2026.134148","url":null,"abstract":"<p><p>The growing global demand for oils and lipids, alongside the environmental impact of traditional oil crop cultivation, has generated significant interest in fermentation (or specifically precision fermentation) of oleaginous yeasts to produce food-grade bio-oils. While research is growing in upstream processing and innovation, downstream processing, which includes cell harvesting, disruption, oil extraction, and purification, remains underexplored. Current downstream methods are largely chemical based due to their high yields, established protocols, and operational simplicity. However, these current methods raise concerns regarding food safety and environmental sustainability. This review provides a comprehensive overview of these challenges across the various stages of downstream processing and examines their impact on process efficiency, sustainability, and scalability. The review also identifies key research gaps and proposes future research directions to advance the field and takes a whole-system approach to the sustainable production of food grade yeast oils. Furthermore, the review proposes novel research paths by analysing and drawing inspiration from oil recovery in biorefinery research. Developing efficient downstream processes for yeast-derived oils presents several challenges, including complex bio-separation steps, limited sustainable alternatives, high capital and energy requirements, scalability issues, and a lack of integrated process understanding. There are promising strategies from biorefinery research, such as innovations in solvents, novel adsorption techniques, advances in membrane technology and insitu conversion to finished products, which could be adapted (considering appropriate regulatory frameworks) to process food-grade yeast oils. The insights within this review aim to support the development of scalable, safe, and sustainable downstream processes to meet the rising demand for alternative oils.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"134148"},"PeriodicalIF":9.0,"publicationDate":"2026-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146130477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-02-03DOI: 10.1016/j.biortech.2026.134155
Yaofeng Hu, Yifan Gui, Wei Li, Cuiling Gong, Ziqi Hou, Tong Shu, Ya Wu, Gen Lu, Longjiang Yu
{"title":"High-efficiency restoration of stone cultural relics via immobilized carbonic anhydrase on magnetic graphite oxide","authors":"Yaofeng Hu, Yifan Gui, Wei Li, Cuiling Gong, Ziqi Hou, Tong Shu, Ya Wu, Gen Lu, Longjiang Yu","doi":"10.1016/j.biortech.2026.134155","DOIUrl":"https://doi.org/10.1016/j.biortech.2026.134155","url":null,"abstract":"","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"40 1","pages":""},"PeriodicalIF":11.4,"publicationDate":"2026-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146109828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-02-03DOI: 10.1016/j.biortech.2026.134154
Yaxin Wang , Yifan Xiao , Ruixin Li , Mark Bartlam , Yingying Wang
As farmland represents the largest nitrous oxide (N2O) emitter, widely used chiral pesticides frequently coexist with N2O. To explore the underlying molecular mechanisms of chiral pesticides regulating N2O emissions, this study experimented with Paracoccus denitrificans PD1222 on anaerobic/aerobic denitrification under cis-epoxiconazole (EPO) stress. The (+)-EPO increased 13.51-fold N2O emissions under anaerobic denitrification by inhibiting the denitrification genes, while (−)-EPO merely enhanced 4.22-fold N2O emissions through improving electron transfer and nitrous oxide reductase assembly. Anaerobic conditions suppressed glucose metabolism and NADH production, disrupting the energy supply for denitrification under EPO stress. Aerobic conditions hindered electron transfer and intracellular iron transport, reducing denitrifying enzyme activity and causing a further 1.29–10.47-fold increase in N2O emissions and extra nitrite accumulation. These findings revealed that oxygen intensified the ecological risk of cis-epoxiconazole monomers stimulating N2O emissions and underscore the potential risks of agrochemical applications to climate change and ecosystem stability.
{"title":"Chiral pesticides stereoselectively accelerate N2O emission through aerobic denitrification","authors":"Yaxin Wang , Yifan Xiao , Ruixin Li , Mark Bartlam , Yingying Wang","doi":"10.1016/j.biortech.2026.134154","DOIUrl":"10.1016/j.biortech.2026.134154","url":null,"abstract":"<div><div>As farmland represents the largest nitrous oxide (N<sub>2</sub>O) emitter, widely used chiral pesticides frequently coexist with N<sub>2</sub>O. To explore the underlying molecular mechanisms of chiral pesticides regulating N<sub>2</sub>O emissions, this study experimented with <em>Paracoccus denitrificans</em> PD1222 on anaerobic/aerobic denitrification under <em>cis</em>-epoxiconazole (EPO) stress. The (+)-EPO increased 13.51-fold N<sub>2</sub>O emissions under anaerobic denitrification by inhibiting the denitrification genes, while (−)-EPO merely enhanced 4.22-fold N<sub>2</sub>O emissions through improving electron transfer and nitrous oxide reductase assembly. Anaerobic conditions suppressed glucose metabolism and NADH production, disrupting the energy supply for denitrification under EPO stress. Aerobic conditions hindered electron transfer and intracellular iron transport, reducing denitrifying enzyme activity and causing a further 1.29–10.47-fold increase in N<sub>2</sub>O emissions and extra nitrite accumulation. These findings revealed that oxygen intensified the ecological risk of <em>cis</em>-epoxiconazole monomers stimulating N<sub>2</sub>O emissions and underscore the potential risks of agrochemical applications to climate change and ecosystem stability.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"446 ","pages":"Article 134154"},"PeriodicalIF":9.0,"publicationDate":"2026-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146109829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-02-02DOI: 10.1016/j.biortech.2026.134147
Long Huang , Lin Liu , Guangyi Zhang , Guoqiang Li , Yingke Fang , Yuan Li , Huiying Yang , Hongbin Xu
Polyhydroxyalkanoates (PHAs) are promising substitutes for petroleum-based plastics, but their production is constrained by the high energy demand and CO2 emissions of mechanical aeration. We developed a membrane-mediated, photosynthetically coupled system in which a hydrophobic polytetrafluoroethylene (PTFE) membrane enables gas exchange between spatially separated microalgal and PHA-storing mixed-culture chambers, establishing an internal O2–CO2 cycle. The effects of microbial-to-algal biomass ratio (Mp/Ma), substrate-to-microbe ratio (F/M), membrane area-to-volume ratio (θ) and initial inorganic carbon concentration (IC_ini) were evaluated. Optimal performance was achieved at Mp/Ma = 3:1 and F/M = 1:1; increasing θ to 0.012 m2 L−1 allowed microalgal oxygen to sustain a PHA content of 51% (VSS), comparable to mechanical aeration. Under these conditions, specific energy consumption and process-related CO2 emissions per unit PHA were reduced by 90% and 38%, respectively, with microalgal CO2 fixation contributing 11%, rising to 16.7% at 30 mmol L−1 inorganic carbon. This configuration offers a promising route toward low-energy, low-carbon PHA production.
{"title":"A membrane-mediated algal–bacterial coupling strategy for energy – efficient and low-carbon PHA production","authors":"Long Huang , Lin Liu , Guangyi Zhang , Guoqiang Li , Yingke Fang , Yuan Li , Huiying Yang , Hongbin Xu","doi":"10.1016/j.biortech.2026.134147","DOIUrl":"10.1016/j.biortech.2026.134147","url":null,"abstract":"<div><div>Polyhydroxyalkanoates (PHAs) are promising substitutes for petroleum-based plastics, but their production is constrained by the high energy demand and CO<sub>2</sub> emissions of mechanical aeration. We developed a membrane-mediated, photosynthetically coupled system in which a hydrophobic polytetrafluoroethylene (PTFE) membrane enables gas exchange between spatially separated microalgal and PHA-storing mixed-culture chambers, establishing an internal O<sub>2</sub>–CO<sub>2</sub> cycle. The effects of microbial-to-algal biomass ratio (Mp/Ma), substrate-to-microbe ratio (F/M), membrane area-to-volume ratio (θ) and initial inorganic carbon concentration (IC_ini) were evaluated. Optimal performance was achieved at Mp/Ma = 3:1 and F/M = 1:1; increasing θ to 0.012 m<sup>2</sup> L<sup>−1</sup> allowed microalgal oxygen to sustain a PHA content of 51% (VSS), comparable to mechanical aeration. Under these conditions, specific energy consumption and process-related CO<sub>2</sub> emissions per unit PHA were reduced by 90% and 38%, respectively, with microalgal CO<sub>2</sub> fixation contributing 11%, rising to 16.7% at 30 mmol L<sup>−1</sup> inorganic carbon. This configuration offers a promising route toward low-energy, low-carbon PHA production.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"446 ","pages":"Article 134147"},"PeriodicalIF":9.0,"publicationDate":"2026-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146109830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
扫码关注我们
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号