Pub Date : 2024-11-12DOI: 10.1016/j.biortech.2024.131803
Lars Halle , Daniela Höppner , Marvin Doser , Christian Brüsseler , Jochem Gätgens , Niclas Conen , Andreas Jupke , Jan Marienhagen , Stephan Noack
α-ketoglutarate (AKG) is a valuable dicarboxylic acid with multiple applications in the food, pharmaceutical, and chemical industries. Its chemical synthesis is associated with toxic by-products, low specificity, and high energy input. To create a more environmentally friendly and sustainable alternative, a microbial production process for AKG was developed. Four potential producer strains were generated by metabolic engineering of Corynebacterium glutamicum and characterized on defined glucose/sucrose media as well as molasses, a side stream from sugar beet processing. While strain C. glutamicum PO6-iolT1 Δgdh ΔgltB mscCG’ ΔodhA was not able to grow on defined media it outperformed all predecessor variants on molasses. Successful scale-up into a fed-batch bioreactor process with molasses yielded 96.2 g AKG with a conversion yield of 0.64 g g−1. Finally, downstream processing by liquid–liquid extraction with ethyl acetate enabled product purification with an extraction efficiency of 87 % and an AKG purity of > 93 %.
{"title":"From molasses to purified α-ketoglutarate with engineered Corynebacterium glutamicum","authors":"Lars Halle , Daniela Höppner , Marvin Doser , Christian Brüsseler , Jochem Gätgens , Niclas Conen , Andreas Jupke , Jan Marienhagen , Stephan Noack","doi":"10.1016/j.biortech.2024.131803","DOIUrl":"10.1016/j.biortech.2024.131803","url":null,"abstract":"<div><div>α-ketoglutarate (AKG) is a valuable dicarboxylic acid with multiple applications in the food, pharmaceutical, and chemical industries. Its chemical synthesis is associated with toxic by-products, low specificity, and high energy input. To create a more environmentally friendly and sustainable alternative, a microbial production process for AKG was developed. Four potential producer strains were generated by metabolic engineering of <em>Corynebacterium<!--> <!-->glutamicum</em> and characterized on defined glucose/sucrose media as well as molasses, a side stream from sugar beet processing. While strain <em>C.<!--> <!-->glutamicum</em> P<sub>O6</sub><em>-iolT</em>1<!--> <!-->Δ<em>gdh</em> Δ<em>gltB mscCG</em>’ Δ<em>odhA</em> was not able to grow on defined media it outperformed all predecessor variants on molasses. Successful scale-up into a fed-batch bioreactor process with molasses yielded 96.2<!--> <!-->g AKG with a conversion yield of 0.64<!--> <!-->g<!--> <!-->g<sup>−1</sup>. Finally, downstream processing by liquid–liquid extraction with ethyl acetate enabled product purification with an extraction efficiency of 87 % and an AKG purity of > 93 %.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"416 ","pages":"Article 131803"},"PeriodicalIF":9.7,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142613429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-12DOI: 10.1016/j.biortech.2024.131816
Wenjing Yang, Shuzhen Li, Shuai Gao, Hui Zhong, Zhiguo He
Polyhydroxyalkanoates (PHAs) are intracellular storage polymers that enhance bacterial resistance in environments. While the role of PHAs regulation in thermophiles under high-temperature stimulation is understudied, this work investigates Aeribacillus pallidus BK1, a thermophile with heat resistance up to 155 °C. Our results showed that A. pallidus's PHAs yield was 1.45 g/L. After 90 °C and 121 °C stimulations, the PHAs yield doubled to 3.33 g/L. The PHAs ratios increased from 35.63 % (60 °C) to 75.46 % (90 °C) and 77.15 % (121 °C). RNA-seq analysis revealed a common strategy of activating glucose transporters to enhance glucose uptake at both temperatures. At 90 °C, A. pallidus BK1 prioritized PHAs accumulation over the TCA cycle. At 121 °C, PHAs production was further enhanced by upregulating monomer polymerization and downregulating acetyl-CoA carboxylase expression. These findings offered valuable insights into the high-temperature defense mechanisms of thermophiles and suggested that A. pallidus BK1 holds promise as a bio-production platform for PHAs production under thermal stimulation.
{"title":"High-Temperature stimulation enhances Polyhydroxyalkanoates accumulation in thermophile Aeribacillus pallidus BK1.","authors":"Wenjing Yang, Shuzhen Li, Shuai Gao, Hui Zhong, Zhiguo He","doi":"10.1016/j.biortech.2024.131816","DOIUrl":"https://doi.org/10.1016/j.biortech.2024.131816","url":null,"abstract":"<p><p>Polyhydroxyalkanoates (PHAs) are intracellular storage polymers that enhance bacterial resistance in environments. While the role of PHAs regulation in thermophiles under high-temperature stimulation is understudied, this work investigates Aeribacillus pallidus BK1, a thermophile with heat resistance up to 155 °C. Our results showed that A. pallidus's PHAs yield was 1.45 g/L. After 90 °C and 121 °C stimulations, the PHAs yield doubled to 3.33 g/L. The PHAs ratios increased from 35.63 % (60 °C) to 75.46 % (90 °C) and 77.15 % (121 °C). RNA-seq analysis revealed a common strategy of activating glucose transporters to enhance glucose uptake at both temperatures. At 90 °C, A. pallidus BK1 prioritized PHAs accumulation over the TCA cycle. At 121 °C, PHAs production was further enhanced by upregulating monomer polymerization and downregulating acetyl-CoA carboxylase expression. These findings offered valuable insights into the high-temperature defense mechanisms of thermophiles and suggested that A. pallidus BK1 holds promise as a bio-production platform for PHAs production under thermal stimulation.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"131816"},"PeriodicalIF":9.7,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142613431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-12DOI: 10.1016/j.biortech.2024.131806
Sangdo Yook , Anshu Deewan , Leah Ziolkowski , Stephan Lane , Payman Tohidifar , Ming-Hsun Cheng , Vijay Singh , Matthew J. Stasiewicz , Christopher V. Rao , Yong-Su Jin
Yarrowia lipolytica, an oleaginous yeast, shows promise for industrial fermentation due to its robust acetyl-CoA flux and well-developed genetic engineering tools. However, its lack of an active xylose metabolism restricts the conversion of cellulosic sugars to valuable products. To address this, metabolic engineering, and adaptive laboratory evolution (ALE) were applied to the Y. lipolytica PO1f strain, resulting in an efficient xylose-assimilating strain (XEV). Whole-genome sequencing (WGS) of the XEV followed by reverse engineering revealed that the amplification of the heterologous oxidoreductase pathway and a mutation in the GTPase-activating protein gene (YALI0B12100g) might be the primary reasons for improved xylose assimilation in the XEV strain. When a sorghum hydrolysate was used, the XEV strain showed superior xylose consumption and lipid production compared to its parental strain (X123). This study advances our understanding of xylose metabolism in Y. lipolytica and proposes effective metabolic engineering strategies for optimizing lignocellulosic hydrolysates.
{"title":"Engineering and evolution of Yarrowia lipolytica for producing lipids from lignocellulosic hydrolysates","authors":"Sangdo Yook , Anshu Deewan , Leah Ziolkowski , Stephan Lane , Payman Tohidifar , Ming-Hsun Cheng , Vijay Singh , Matthew J. Stasiewicz , Christopher V. Rao , Yong-Su Jin","doi":"10.1016/j.biortech.2024.131806","DOIUrl":"10.1016/j.biortech.2024.131806","url":null,"abstract":"<div><div><em>Yarrowia lipolytica</em>, an oleaginous yeast, shows promise for industrial fermentation due to its robust acetyl-CoA flux and well-developed genetic engineering tools. However, its lack of an active xylose metabolism restricts the conversion of cellulosic sugars to valuable products. To address this, metabolic engineering, and adaptive laboratory evolution (ALE) were applied to the <em>Y. lipolytica</em> PO1f strain, resulting in an efficient xylose-assimilating strain (XEV). Whole-genome sequencing (WGS) of the XEV followed by reverse engineering revealed that the amplification of the heterologous oxidoreductase pathway and a mutation in the GTPase-activating protein gene (YALI0B12100g) might be the primary reasons for improved xylose assimilation in the XEV strain. When a sorghum hydrolysate was used, the XEV strain showed superior xylose consumption and lipid production compared to its parental strain (X123). This study advances our understanding of xylose metabolism in <em>Y. lipolytica</em> and proposes effective metabolic engineering strategies for optimizing lignocellulosic hydrolysates.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"416 ","pages":"Article 131806"},"PeriodicalIF":9.7,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142613415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-12DOI: 10.1016/j.biortech.2024.131800
Liang Ji , Chenni Zhao , Yulong He , Yuchen Yuan , Zhiwei Hong , Liyun Sun , Jianhua Fan
Microalgae not only fix carbon dioxide, but also represent a promising alternative resource for the production of proteins, lipids, and polysaccharides. This study employed two Porphyridium strains to compare their responses under different light qualities. P. purpureum up-regulated the content (up to 69.37 ± 0.92 mg/g DW) and proportion of phycoerythrin to enhance light absorption, which led to the accumulation of total soluble proteins, neutral lipids and exopolysaccharides under blue light. In contrast, P. aerugineum primarily improved the light energy utilization by increasing phycocyanin levels (up to 81.10 ± 0.60 mg/g DW), resulting in the degradation of neutral lipids and the accumulation of exopolysaccharides. Given the biomass, the highest yields of phycoerythrin (169.61 ± 2.90 mg/L) and phycocyanin (216.92 ± 1.90 mg/L) were achieved by P. purpureum and P. aerugineum cultured under white light, respectively. These findings indicate that Porphyridium can serve as a valuable resource for phycobiliprotein production, with biomolecules synthesis being tightly regulated by light quality.
{"title":"Exploring Porphyridium purpureum and Porphyridium aerugineum as alternative resources for phycobiliprotein production","authors":"Liang Ji , Chenni Zhao , Yulong He , Yuchen Yuan , Zhiwei Hong , Liyun Sun , Jianhua Fan","doi":"10.1016/j.biortech.2024.131800","DOIUrl":"10.1016/j.biortech.2024.131800","url":null,"abstract":"<div><div>Microalgae not only fix carbon dioxide, but also represent a promising alternative resource for the production of proteins, lipids, and polysaccharides. This study employed two <em>Porphyridium</em> strains to compare their responses under different light qualities. <em>P. purpureum</em> up-regulated the content (up to 69.37 ± 0.92 mg/g DW) and proportion of phycoerythrin to enhance light absorption, which led to the accumulation of total soluble proteins, neutral lipids and exopolysaccharides under blue light. In contrast, <em>P. aerugineum</em> primarily improved the light energy utilization by increasing phycocyanin levels (up to 81.10 ± 0.60 mg/g DW), resulting in the degradation of neutral lipids and the accumulation of exopolysaccharides. Given the biomass, the highest yields of phycoerythrin (169.61 ± 2.90 mg/L) and phycocyanin (216.92 ± 1.90 mg/L) were achieved by <em>P. purpureum</em> and <em>P. aerugineum</em> cultured under white light, respectively. These findings indicate that <em>Porphyridium</em> can serve as a valuable resource for phycobiliprotein production, with biomolecules synthesis being tightly regulated by light quality.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"416 ","pages":"Article 131800"},"PeriodicalIF":9.7,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142613416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-12DOI: 10.1016/j.biortech.2024.131814
Jose M Orts, Emilia Naranjo, Susana Pina, Angel Orts, Marta Muñoz-Martí, Manuel Tejada, Juan Parrado
A circular economy process has been developed to convert polyurethane waste into biofertilizing microorganisms through a sequential chemical/biological process. The chemical phase involves the complete depolymerization of polyurethane using ozone attack, generating an aqueous extract (OLE) composed of small, bioavailable molecules such as polyols, isocyanate derivatives, and carboxylic acids. The biological phase utilizes OLE for the generation of biomass with biofertilizing functional activity through Rhodococcus pyridinivorans fermentation. The metabolic-proteomic expression during the biodegradation of OLE involves the synthesis of numerous enzymes such as cutinases, hydrolases, proteases, esterases and oxidoreductases, which participate in the degradation of chemical compounds like benzene derivatives, phenols, or plastic polymers. OLE has been converted into microorganisms with biofertilizing properties, including nitrogen fixation, phytohormone production and siderophores. This process contributes to sustainability by diverting polyurethane waste from landfills, reducing the environmental impact of chemical fertilizers and promoting a more sustainable agricultural system.
我们开发了一种循环经济工艺，通过一个连续的化学/生物工艺将聚氨酯废料转化为生物肥料微生物。化学阶段包括利用臭氧攻击使聚氨酯完全解聚，生成由多元醇、异氰酸酯衍生物和羧酸等生物可利用的小分子组成的水提取物（OLE）。生物阶段利用 OLE，通过 Rhodococcus pyridinivorans 发酵产生具有生物肥料功能活性的生物质。在 OLE 的生物降解过程中，新陈代谢-蛋白质组的表达涉及多种酶的合成，如切朊酶、水解酶、蛋白酶、酯酶和氧化还原酶，这些酶参与苯衍生物、酚类或塑料聚合物等化合物的降解。OLE 已转化为具有生物肥料特性的微生物，包括固氮、产生植物激素和嗜苷酶。这一工艺可将聚氨酯废料从垃圾填埋场转移出来，减少化肥对环境的影响，促进农业系统的可持续发展。
{"title":"Polyurethane waste Valorization: A Two-Phase process using Ozonization and Rhodococcus pyridinivorans fermentation for biofertilizer production.","authors":"Jose M Orts, Emilia Naranjo, Susana Pina, Angel Orts, Marta Muñoz-Martí, Manuel Tejada, Juan Parrado","doi":"10.1016/j.biortech.2024.131814","DOIUrl":"https://doi.org/10.1016/j.biortech.2024.131814","url":null,"abstract":"<p><p>A circular economy process has been developed to convert polyurethane waste into biofertilizing microorganisms through a sequential chemical/biological process. The chemical phase involves the complete depolymerization of polyurethane using ozone attack, generating an aqueous extract (OLE) composed of small, bioavailable molecules such as polyols, isocyanate derivatives, and carboxylic acids. The biological phase utilizes OLE for the generation of biomass with biofertilizing functional activity through Rhodococcus pyridinivorans fermentation. The metabolic-proteomic expression during the biodegradation of OLE involves the synthesis of numerous enzymes such as cutinases, hydrolases, proteases, esterases and oxidoreductases, which participate in the degradation of chemical compounds like benzene derivatives, phenols, or plastic polymers. OLE has been converted into microorganisms with biofertilizing properties, including nitrogen fixation, phytohormone production and siderophores. This process contributes to sustainability by diverting polyurethane waste from landfills, reducing the environmental impact of chemical fertilizers and promoting a more sustainable agricultural system.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"131814"},"PeriodicalIF":9.7,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142610755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-12DOI: 10.1016/j.biortech.2024.131802
Zhenqiang Zhao , Jiajia You , Xuanping Shi , Mengmeng Cai , Rongshuai Zhu , Fengyu Yang , Meijuan Xu , Minglong Shao , Rongzhen Zhang , Youxi Zhao , Zhiming Rao
The rapid development of high-productivity strains is fundamental for bio-manufacture in industry. Here, Multi-module metabolic engineering was implemented to reprogram Escherichia coli, enabling it to rapidly transitioning from zero-producer to hyperproducer of L-threonine. Firstly, the synthesis pathway of L-threonine was rationally divided into five modules, and the rapid production of L-threonine was achieved by optimizing the expression of genes in each module. Subsequently, the capture and fixation of CO2 was enhanced to improve L-threonine yield. Dynamically balancing cell growth and yield by quorum-sensing system resulted in the accumulation of L-threonine up to 34.24 g/L. Ultimately, the THR36-L19 strain accumulated 120.1 g/L L-threonine with 0.425 g/g glucose in a 5 L bioreactor. This is the highest yield for de novo producing L-threonine reported to date and without the use of exogenous inducers and antibiotics in the fermentation process. It also provided an effective technological guidence for the zero-to-overproduction of other chemicals.
{"title":"Multi-module engineering to guide the development of an efficient L-threonine-producing cell factory","authors":"Zhenqiang Zhao , Jiajia You , Xuanping Shi , Mengmeng Cai , Rongshuai Zhu , Fengyu Yang , Meijuan Xu , Minglong Shao , Rongzhen Zhang , Youxi Zhao , Zhiming Rao","doi":"10.1016/j.biortech.2024.131802","DOIUrl":"10.1016/j.biortech.2024.131802","url":null,"abstract":"<div><div>The rapid development of high-productivity strains is fundamental for bio-manufacture in industry. Here, Multi-module metabolic engineering was implemented to reprogram <em>Escherichia coli</em>, enabling it to rapidly transitioning from zero-producer to hyperproducer of L-threonine. Firstly, the synthesis pathway of L-threonine was rationally divided into five modules, and the rapid production of L-threonine was achieved by optimizing the expression of genes in each module. Subsequently, the capture and fixation of CO<sub>2</sub> was enhanced to improve L-threonine yield. Dynamically balancing cell growth and yield by quorum-sensing system resulted in the accumulation of L-threonine up to 34.24 g/L. Ultimately, the THR36-L19 strain accumulated 120.1 g/L L-threonine with 0.425 g/g glucose in a 5 L bioreactor. This is the highest yield for <em>de novo</em> producing L-threonine reported to date and without the use of exogenous inducers and antibiotics in the fermentation process. It also provided an effective technological guidence for the zero-to-overproduction of other chemicals.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"416 ","pages":"Article 131802"},"PeriodicalIF":9.7,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142610779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
To solve the problems of antibiotic pollution, water resources and energy shortage, an osmotic microbial fuel cell (OsMFC) was adopted innovatively to treat antibiotic wastewater containing sulfamethoxazole (SMX), and achieved SMX removal, water production and electricity generation. Substrate concentration was a key factor affecting the performances of OsMFC, which was often ignored by researchers. This study explored the effect of substrate concentration on system performances, clarified the dynamic changes of membrane fouling under different substrate concentrations, and further revealed the response of microbial communities. The results showed that the stable removal efficiency of SMX exceeded 98.8 % due to the efficient interception of forward osmosis (FO) membrane. Compared with the 1.0 g/L NaAc system, the SMX degradation efficiency and maximum output voltage in the 2.0 g/L NaAc system were only increased by 3.9 % and 6.3 %, respectively. However, the initial water flux decreased by 30.1 % in the 7th cycle due to more serious FO membrane fouling. In addition, there were significant differences in the dynamic formation process of FO membrane fouling. Higher substrate concentration increased the relative abundance of Desulfobacterota and Geobacter. Functional prediction analysis showed that increasing substrate concentration promoted carbohydrate metabolism pathways and relative abundance of sulfur respiration functional groups, thereby improving COD and SMX removal rates. However, the biosynthesis of other secondary metabolites was significantly improved, resulting in increased contents of EPS and SMP, which aggravated membrane pollution. Overall, the system performed better when the substrate concentration was 1.0 g/L. This study would provide certain guidance for the performance optimization and membrane fouling mitigation of OsMFC, thereby promoting its practical application in antibiotic wastewater treatment.
{"title":"Effect of substrate concentration on sulfamethoxazole wastewater treatment by osmotic microbial fuel cell: Insight into operational efficiency, dynamic changes of membrane fouling and microbial response.","authors":"Hengliang Zhang, Fei Xing, Liang Duan, Qiusheng Gao, Shilong Li, Yang Zhao","doi":"10.1016/j.biortech.2024.131805","DOIUrl":"https://doi.org/10.1016/j.biortech.2024.131805","url":null,"abstract":"<p><p>To solve the problems of antibiotic pollution, water resources and energy shortage, an osmotic microbial fuel cell (OsMFC) was adopted innovatively to treat antibiotic wastewater containing sulfamethoxazole (SMX), and achieved SMX removal, water production and electricity generation. Substrate concentration was a key factor affecting the performances of OsMFC, which was often ignored by researchers. This study explored the effect of substrate concentration on system performances, clarified the dynamic changes of membrane fouling under different substrate concentrations, and further revealed the response of microbial communities. The results showed that the stable removal efficiency of SMX exceeded 98.8 % due to the efficient interception of forward osmosis (FO) membrane. Compared with the 1.0 g/L NaAc system, the SMX degradation efficiency and maximum output voltage in the 2.0 g/L NaAc system were only increased by 3.9 % and 6.3 %, respectively. However, the initial water flux decreased by 30.1 % in the 7th cycle due to more serious FO membrane fouling. In addition, there were significant differences in the dynamic formation process of FO membrane fouling. Higher substrate concentration increased the relative abundance of Desulfobacterota and Geobacter. Functional prediction analysis showed that increasing substrate concentration promoted carbohydrate metabolism pathways and relative abundance of sulfur respiration functional groups, thereby improving COD and SMX removal rates. However, the biosynthesis of other secondary metabolites was significantly improved, resulting in increased contents of EPS and SMP, which aggravated membrane pollution. Overall, the system performed better when the substrate concentration was 1.0 g/L. This study would provide certain guidance for the performance optimization and membrane fouling mitigation of OsMFC, thereby promoting its practical application in antibiotic wastewater treatment.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"131805"},"PeriodicalIF":9.7,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142613413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-12DOI: 10.1016/j.biortech.2024.131749
Abdelhani Chaabna, Samia Semcheddine
This paper introduces a robust control approach based on the Sliding Mode Control (SMC) strategy for the Anaerobic Digestion (AD) process. The Anaerobic Digestion Model N°1 (ADM1), the most detailed and comprehensive model in this field, is used as a virtual AD plant. The proposed control scheme is derived from a reduced model. This control law utilizes the state variables of the reduced AM2HN model along with the stoichiometric and kinetic parameters. An interface block between the ADM1 and AM2HN state variables has been designed in a closed-loop configuration. The performance of SMC has been enhanced through the use of a low-pass filter, which completely eliminates chattering, providing smooth, chatter-free dilution rates while preserving the rapid convergence to target methane production (within less than 5 days) even in a noisy environment.
{"title":"Robust methane production from anaerobic digestion of maize silage: Feeding control using sliding mode control strategy with Anaerobic Digestion Model N°01","authors":"Abdelhani Chaabna, Samia Semcheddine","doi":"10.1016/j.biortech.2024.131749","DOIUrl":"10.1016/j.biortech.2024.131749","url":null,"abstract":"<div><div>This paper introduces a robust control approach based on the Sliding Mode Control (SMC) strategy for the Anaerobic Digestion (AD) process. The Anaerobic Digestion Model N°1 (ADM1), the most detailed and comprehensive model in this field, is used as a virtual AD plant. The proposed control scheme is derived from a reduced model. This control law utilizes the state variables of the reduced AM2HN model along with the stoichiometric and kinetic parameters. An interface block between the ADM1 and AM2HN state variables has been designed in a closed-loop configuration. The performance of SMC has been enhanced through the use of a low-pass filter, which completely eliminates chattering, providing smooth, chatter-free dilution rates while preserving the rapid convergence to target methane production (within less than 5 days) even in a noisy environment.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"416 ","pages":"Article 131749"},"PeriodicalIF":9.7,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142610757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-11DOI: 10.1016/j.biortech.2024.131798
Yousaf Ayub, Jianzhao Zhou, Tao Shi, Sara Toniolo, Jingzheng Ren
This study evaluated three blue hydrogen production processes - solar-grid powered, wind-powered, and thermal power grid (TPG) - considering the context of Hong Kong, SAR, China. A process sustainability analysis was performed based on energy, economic, and environmental (3E) factors. The energy efficiency analysis indicates that the TPG system is the most energy-efficient with 64% efficiency, followed by the wind power system at 63% and the hybrid solar-grid powered system at 60%. The economic analysis results indicate that the levelized cost of hydrogen (LCH) is 2.165 $/kg for the TPG system, 2.132 $/kg for the hybrid solar-grid powered system, and 2.060 $/kg for the wind power system. The environmental assessment suggests that wind powered are eco-friendly with a unit point total (µPt) of 1.11, compared to the solar-grid 1.50 µPt and TPG system's 1.70 µPt. Therefore, 3E analysis proposes wind powered process is more sustainable for blue H2 production.
{"title":"Sustainability assessment of blue hydrogen production through biomass gasification: A comparative analysis of thermal, solar, and wind energy sources.","authors":"Yousaf Ayub, Jianzhao Zhou, Tao Shi, Sara Toniolo, Jingzheng Ren","doi":"10.1016/j.biortech.2024.131798","DOIUrl":"https://doi.org/10.1016/j.biortech.2024.131798","url":null,"abstract":"<p><p>This study evaluated three blue hydrogen production processes - solar-grid powered, wind-powered, and thermal power grid (TPG) - considering the context of Hong Kong, SAR, China. A process sustainability analysis was performed based on energy, economic, and environmental (3E) factors. The energy efficiency analysis indicates that the TPG system is the most energy-efficient with 64% efficiency, followed by the wind power system at 63% and the hybrid solar-grid powered system at 60%. The economic analysis results indicate that the levelized cost of hydrogen (LCH) is 2.165 $/kg for the TPG system, 2.132 $/kg for the hybrid solar-grid powered system, and 2.060 $/kg for the wind power system. The environmental assessment suggests that wind powered are eco-friendly with a unit point total (µPt) of 1.11, compared to the solar-grid 1.50 µPt and TPG system's 1.70 µPt. Therefore, 3E analysis proposes wind powered process is more sustainable for blue H<sub>2</sub> production.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"131798"},"PeriodicalIF":9.7,"publicationDate":"2024-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142610759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-10DOI: 10.1016/j.biortech.2024.131801
Jia-Cong Huang , Qi Guo , Xu-Hong Li, Tian-Qiong Shi
The development of high-performance strains and the continuous breakthrough of strain screening technology also pose challenges to downstream fermentation optimization and scale-up. Therefore, neural network models are utilized to optimize the fermentation process to meet the goals of boosting yield or lowering cost, with the use of artificial intelligence technology in conjunction with the peculiarities of the fermentation process. High-performance strains’ yield rise and fermentation process amplification will be sped up with the aid of neural network models. This paper offers a helpful review for anyone interested in state-of-the-art microbial fermentation processes, as it thoroughly reviews the application of neural network models in predicting fermentation yield, optimizing the fermentation process, and monitoring the fermentation process.
{"title":"A comprehensive review on the application of neural network model in microbial fermentation","authors":"Jia-Cong Huang , Qi Guo , Xu-Hong Li, Tian-Qiong Shi","doi":"10.1016/j.biortech.2024.131801","DOIUrl":"10.1016/j.biortech.2024.131801","url":null,"abstract":"<div><div>The development of high-performance strains and the continuous breakthrough of strain screening technology also pose challenges to downstream fermentation optimization and scale-up. Therefore, neural network models are utilized to optimize the fermentation process to meet the goals of boosting yield or lowering cost, with the use of artificial intelligence technology in conjunction with the peculiarities of the fermentation process. High-performance strains’ yield rise and fermentation process amplification will be sped up with the aid of neural network models. This paper offers a helpful review for anyone interested in state-of-the-art microbial fermentation processes, as it thoroughly reviews the application of neural network models in predicting fermentation yield, optimizing the fermentation process, and monitoring the fermentation process.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"416 ","pages":"Article 131801"},"PeriodicalIF":9.7,"publicationDate":"2024-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142610764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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