Pub Date : 2026-01-29DOI: 10.1016/j.biortech.2026.134127
Wenzhen Guan , Yifeng Xu , Shengjun Li , Linchuan Fang , Yiwen Liu , Bingjie Ni , Lai Peng
Ammonia-oxidizing archaea (AOA) are pivotal in nitrous oxide (N2O) emissions from wastewater systems, though their N2O production mechanisms remain elusive. In this study, AOA-enriched biofilm consortia (58% Candidatus Nitrosocosmicus) were rapidly formed under antibiotic stress. Systematic investigations were conducted to evaluate the impacts of carrier filling ratios (15%, 30%, 60%) and oxygen levels (0.4%, 4.2%, 10.5%, 21%) on N2O production by AOA in biofilm systems. Batch experiments revealed an inverse correlation between oxygen level and N2O emission and demonstrated that 30% filling ratio exhibited the lowest emission factor compared to 15% and 60%. Optimal performance was achieved under 21% oxygen level combined with a 30% filling ratio, yielding a minimal N2O emission factor (0.01%). Adequate oxygen ensured ammonia oxidation completely, while optimal filling ratio allowed uniform substrate transfer and oxygen diffusion. This study will shed new light on greenhouse gas mitigation in biofilm-based wastewater systems.
{"title":"Strategic enrichment of ammonia-oxidizing archaea biofilms coupled with process optimization for mitigating N2O emissions in wastewater treatment system","authors":"Wenzhen Guan , Yifeng Xu , Shengjun Li , Linchuan Fang , Yiwen Liu , Bingjie Ni , Lai Peng","doi":"10.1016/j.biortech.2026.134127","DOIUrl":"10.1016/j.biortech.2026.134127","url":null,"abstract":"<div><div>Ammonia-oxidizing archaea (AOA) are pivotal in nitrous oxide (N<sub>2</sub>O) emissions from wastewater systems, though their N<sub>2</sub>O production mechanisms remain elusive. In this study, AOA-enriched biofilm consortia (58% <em>Candidatus Nitrosocosmicus</em>) were rapidly formed under antibiotic stress. Systematic investigations were conducted to evaluate the impacts of carrier filling ratios (15%, 30%, 60%) and oxygen levels (0.4%, 4.2%, 10.5%, 21%) on N<sub>2</sub>O production by AOA in biofilm systems. Batch experiments revealed an inverse correlation between oxygen level and N<sub>2</sub>O emission and demonstrated that 30% filling ratio exhibited the lowest emission factor compared to 15% and 60%. Optimal performance was achieved under 21% oxygen level combined with a 30% filling ratio, yielding a minimal N<sub>2</sub>O emission factor (0.01%). Adequate oxygen ensured ammonia oxidation completely, while optimal filling ratio allowed uniform substrate transfer and oxygen diffusion. This study will shed new light on greenhouse gas mitigation in biofilm-based wastewater systems.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"446 ","pages":"Article 134127"},"PeriodicalIF":9.0,"publicationDate":"2026-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146072734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-29DOI: 10.1016/j.biortech.2026.134119
Xiang-Lin Chang, Bao-Shan Xing, Yu Qin, Juan Xie, Zhi-Bo Li, Xiaochang C. Wang, Rong Chen, Yu-You Li
{"title":"Sustained performance and microbial succession in novel artificial rumen system coupling dynamic membrane with methanogenic granules for acid absorption","authors":"Xiang-Lin Chang, Bao-Shan Xing, Yu Qin, Juan Xie, Zhi-Bo Li, Xiaochang C. Wang, Rong Chen, Yu-You Li","doi":"10.1016/j.biortech.2026.134119","DOIUrl":"https://doi.org/10.1016/j.biortech.2026.134119","url":null,"abstract":"","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"182 1","pages":""},"PeriodicalIF":11.4,"publicationDate":"2026-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146072741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Mechanism study on the effects of Na/K hydroxides and chlorides on NO reduction by biomass volatiles reburning during high-alkali coal combustion","authors":"Minghui Xu, Jing Zhao, Xiayu Zhu, Honghai Yang, Xiaolin Wei","doi":"10.1016/j.biortech.2026.134083","DOIUrl":"https://doi.org/10.1016/j.biortech.2026.134083","url":null,"abstract":"","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"44 1","pages":""},"PeriodicalIF":11.4,"publicationDate":"2026-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146071702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-27DOI: 10.1016/j.biortech.2026.134104
Sofia Antic Gorrazzi, Sebastian Bonanni, Alejandro Javier Robledo, Diego Ariel Massazza
Biocathode performance is often constrained by low biomass accumulation on the electrode surface due to electrostatic repulsion between negatively charged cells and negatively polarized electrodes. A strategy known as polarity reversal is typically applied to overcome this limitation, initially growing bacteria under anodic conditions and subsequently switching the electrode polarity to cathodic. This approach requires substantial time and requires bacteria capable of bidirectional extracellular electron transfer. In this work, biocathode enhancement is achieved by suppressing electrostatic repulsion between bacteria and the electrode during adhesion stage, via the generation of a positive charge on the electrode through polarization above the potential of zero charge (PZC). Bacterial adhesion kinetics to electrodes polarized at different potentials and subsequent current generation were systematically investigated using a real-time, in situ approach. A fivefold increase in the number of irreversibly adhered bacteria during the first 90 min of polarization was observed on positively charged electrodes compared with negatively charged ones. Kinetic analysis revealed a 63% higher attachment rate in the former case. Subsequent biofilm formation was also enhanced, resulting in cathodic current densities higher than those typically reported for pure cultures. The effectiveness of this strategy was confirmed on gold and carbon-based graphite electrodes, indicating that the underlying mechanism is not material-specific. These findings demonstrate that biocathode development can be improved by a strategy termed here as Surface Charge-Induced Microbial Adhesion (SCIMA), providing a mechanistic framework for optimizing its performance in microbial electrochemical technologies.
{"title":"Enhanced biocathode performance through surface charge induced microbial adhesion","authors":"Sofia Antic Gorrazzi, Sebastian Bonanni, Alejandro Javier Robledo, Diego Ariel Massazza","doi":"10.1016/j.biortech.2026.134104","DOIUrl":"10.1016/j.biortech.2026.134104","url":null,"abstract":"<div><div>Biocathode performance is often constrained by low biomass accumulation on the electrode surface due to electrostatic repulsion between negatively charged cells and negatively polarized electrodes. A strategy known as polarity reversal is typically applied to overcome this limitation, initially growing bacteria under anodic conditions and subsequently switching the electrode polarity to cathodic. This approach requires substantial time and requires bacteria capable of bidirectional extracellular electron transfer. In this work, biocathode enhancement is achieved by suppressing electrostatic repulsion between bacteria and the electrode during adhesion stage, via the generation of a positive charge on the electrode through polarization above the potential of zero charge (PZC). Bacterial adhesion kinetics to electrodes polarized at different potentials and subsequent current generation were systematically investigated using a real-time, <em>in situ</em> approach. A fivefold increase in the number of irreversibly adhered bacteria during the first 90 min of polarization was observed on positively charged electrodes compared with negatively charged ones. Kinetic analysis revealed a 63% higher attachment rate in the former case. Subsequent biofilm formation was also enhanced, resulting in cathodic current densities higher than those typically reported for pure cultures. The effectiveness of this strategy was confirmed on gold and carbon-based graphite electrodes, indicating that the underlying mechanism is not material-specific. These findings demonstrate that biocathode development can be improved by a strategy termed here as Surface Charge-Induced Microbial Adhesion (SCIMA), providing a mechanistic framework for optimizing its performance in microbial electrochemical technologies.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"445 ","pages":"Article 134104"},"PeriodicalIF":9.0,"publicationDate":"2026-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146048104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Artificial regulation of aerobic and anaerobic biofilm thickness is crucial for enhancing nitrogen removal efficiency of the membrane aerated biofilm reactor (MABR). In this study, conductive aeration membrane modules were fabricated by physical weaving technology to couple MABR with microbial electrochemistry for efficient nitrogen removal. Insulating grids of different thickness and conductive carbon fibers were woven onto the aeration membrane to form aerobic and anaerobic layers. When the total biofilm thickness reached 254 μm (150 μm aerobic layer and 104 μm anaerobic layer), the TN removal efficiency (89.49 ± 2.89 %) was optimal. 16S rRNA gene sequencing and metagenomics analysis confirmed that the aerobic and anaerobic layers in the biofilm were completely separated, but there was a synergistic effect in nitrogen removal. The composite cathode structure provides a mechanism for efficient spatial coupling between the aerobic and anaerobic layers, establishing a basis for regulating biofilm stratification.
{"title":"Artificial regulation of aerobic and anaerobic layers interface enhanced efficient nitrogen removal by weaving insulating grid and conductive carbon fiber in membrane aerated biofilm reactor","authors":"Weichao Li, Jingyu Li, Yun Wu, Meixuan Chen, Yangfan Fu, Wei Li, Shuang Liu, Jie Wang, Yingbo Chen","doi":"10.1016/j.biortech.2026.134074","DOIUrl":"https://doi.org/10.1016/j.biortech.2026.134074","url":null,"abstract":"Artificial regulation of aerobic and anaerobic biofilm thickness is crucial for enhancing nitrogen removal efficiency of the membrane aerated biofilm reactor (MABR). In this study, conductive aeration membrane modules were fabricated by physical weaving technology to couple MABR with microbial electrochemistry for efficient nitrogen removal. Insulating grids of different thickness and conductive carbon fibers were woven onto the aeration membrane to form aerobic and anaerobic layers. When the total biofilm thickness reached 254 μm (150 μm aerobic layer and 104 μm anaerobic layer), the TN removal efficiency (89.49 ± 2.89 %) was optimal. 16S rRNA gene sequencing and metagenomics analysis confirmed that the aerobic and anaerobic layers in the biofilm were completely separated, but there was a synergistic effect in nitrogen removal. The composite cathode structure provides a mechanism for efficient spatial coupling between the aerobic and anaerobic layers, establishing a basis for regulating biofilm stratification.","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"44 1","pages":""},"PeriodicalIF":11.4,"publicationDate":"2026-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146048103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-27DOI: 10.1016/j.biortech.2026.134069
Hehe He , Siyu Li , Xuewei Ning , Guiyang Shi , Youran Li
Alkaloids play essential roles in nutrition, therapeutics, and various industrial sectors. However, traditional plant-based extraction methods are limited by environmental sensitivity and inconsistent yields, highlighting the need for more sustainable and reliable production alternatives. Microbial cell factories represent a promising platform for the scalable biosynthesis of alkaloids. This review outlines the fundamental principles of alkaloid biosynthesis and demonstrates how engineering strategies can enhance production efficiency. In addition, biotechnological innovations for expanding and diversifying the structural repertoire of microbially derived alkaloids are examined as a means to unlock novel functionalities. Finally, current challenges and future directions in alkaloid biomanufacturing are discussed, offering broader insights into the microbial production of high-value bio-based compounds. These insights reinforce the central role of biotechnology in advancing sustainable alkaloid biomanufacturing and shaping its future applications.
{"title":"Sustainable biomanufacturing of alkaloids driven by biotechnology: applications, challenges and perspectives","authors":"Hehe He , Siyu Li , Xuewei Ning , Guiyang Shi , Youran Li","doi":"10.1016/j.biortech.2026.134069","DOIUrl":"10.1016/j.biortech.2026.134069","url":null,"abstract":"<div><div>Alkaloids play essential roles in nutrition, therapeutics, and various industrial sectors. However, traditional plant-based extraction methods are limited by environmental sensitivity and inconsistent yields, highlighting the need for more sustainable and reliable production alternatives. Microbial cell factories represent a promising platform for the scalable biosynthesis of alkaloids. This review outlines the fundamental principles of alkaloid biosynthesis and demonstrates how engineering strategies can enhance production efficiency. In addition, biotechnological innovations for expanding and diversifying the structural repertoire of microbially derived alkaloids are examined as a means to unlock novel functionalities. Finally, current challenges and future directions in alkaloid biomanufacturing are discussed, offering broader insights into the microbial production of high-value bio-based compounds. These insights reinforce the central role of biotechnology in advancing sustainable alkaloid biomanufacturing and shaping its future applications.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"445 ","pages":"Article 134069"},"PeriodicalIF":9.0,"publicationDate":"2026-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146048106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}