Bioresource Technology最新文献_第9页

Investigating role of corn stover biochar supplementation on continuous pilot scale anaerobic digestion: Performance and microbial community dynamics 研究玉米秸秆生物炭补充对连续中试规模厌氧消化的作用：性能和微生物群落动态

IF 9.7 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology

Pub Date : 2024-11-06 DOI: 10.1016/j.biortech.2024.131767

Sachin Krushna Bhujbal , Preeti , Akshay Joshi , Pooja Ghosh , Virendra Kumar Vijay

Biochar supplementation could facilitate microbial colonization and increase biogas and methane yield in anaerobic digestion (AD). This study investigated the impact of corn stover biochar (CSB) augmentation on the continuous pilot scale AD of rice straw. The CSB supplementation exhibited the daily average specific biogas and methane yield of 368.6 L/kg volatile solids (VS) and 230 L/kg VS, which were 35% and 37% higher than the control. Principle component analysis indicated that the VS reduction positively correlated with the daily average specific biogas and methane yield. Metagenomic analysis revealed that the CSB supplementation facilitated microbial colonization with the enrichment of unclassified genera from families Planococcaceae, Clostridiaceae, and Ruminococcaceae, and genus Clostridium and methanogenic archaea (Methanosarcina and Methanobacterium). The co-occurrence network revealed a notable shift in microbial interactions following the supplementation of CSB as an additive in AD. These results offer insights for enhancing the performance of the AD using CSB.

在厌氧消化（AD）过程中，补充生物炭可以促进微生物定植，增加沼气和甲烷产量。本研究调查了玉米秸秆生物炭（CSB）添加对稻草连续中试规模厌氧消化的影响。添加 CSB 后，日均特定沼气和甲烷产量分别为 368.6 升/千克挥发性固体（VS）和 230 升/千克 VS，比对照组分别高出 35% 和 37%。主成分分析表明，VS 的减少与日均特定沼气和甲烷产量呈正相关。元基因组分析表明，补充 CSB 促进了微生物的定植，富集了 Planococcaceae、Clostridiaceae 和 Ruminococcaceae 科的未分类菌属，以及梭菌属和产甲烷古细菌（Methanosarcina 和 Methanobacterium）。共现网络显示，在添加 CSB 作为添加剂后，微生物之间的相互作用发生了显著变化。这些结果为利用 CSB 提高厌氧消化工艺的性能提供了启示。

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引用次数: 0

From biomass to power: High-performance reactor design for coking-resistant operation 从生物质到电力：耐焦化运行的高性能反应堆设计。

IF 9.7 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology

Pub Date : 2024-11-06 DOI: 10.1016/j.biortech.2024.131763

Bin Wang , Tong Wang , Dongxu Cui , Tao Li , Rui Xiao

Biomass gasification coupled with solid oxide fuel cell (SOFC) technology utilizes the gas generated from biomass gasification directly as fuel for SOFC, thereby realizing power generation from solid waste. This technology combines the carbon–neutral feature of biomass with the high efficiency and low emissions of SOFC, making it a promising route for clean energy generation. However, biomass gasification syngas possesses a complex composition, including a high concentration of inert gases, which imposes higher requirements on SOFC. This study developed a multi-channel, hierarchical structural design based on the commercial NiO-yttria-stabilized zirconia (YSZ) material system, realizing high-performance power generation using biomass gasification syngas. The results showed that the combination of a unique structural design and an enhanced interface electrochemical reaction effectively mitigates the influence from inert composition dilution. When operating in gasification syngas with nearly 60 % inert components, the power density can reach 2.07 W·cm⁻² (750 °C). In addition, due to the spatial separation of the inert support region and the electrochemically active region, the effect of controlling the position of carbon deposits was achieved, demonstrating 100 h stable operation with dry biomass gasification syngas. Hence, the combination of micro-tubular SOFC with distinctive structural regulation and biomass gasification exhibits promising prospects for further development.

生物质气化与固体氧化物燃料电池（SOFC）技术利用生物质气化产生的气体直接作为 SOFC 的燃料，从而实现固体废弃物发电。该技术将生物质的碳中和特性与 SOFC 的高效率和低排放相结合，是一条前景广阔的清洁能源发电路线。然而，生物质气化合成气成分复杂，包括高浓度的惰性气体，这对 SOFC 提出了更高的要求。本研究开发了一种基于商用氧化镍-钇稳定氧化锆（YSZ）材料体系的多通道分层结构设计，实现了利用生物质气化合成气的高性能发电。研究结果表明，独特的结构设计与增强的界面电化学反应相结合，有效缓解了惰性成分稀释的影响。在惰性成分占近 60% 的气化合成气中运行时，功率密度可达 2.07 W-cm-2（750 °C）。此外，由于惰性支撑区和电化学活性区在空间上的分离，还实现了控制碳沉积位置的效果，在干燥的生物质气化合成气中稳定运行了 100 小时。因此，具有独特结构调节的微管 SOFC 与生物质气化的结合具有广阔的发展前景。

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引用次数: 0

Modeling the effect of external load variations on single, serie and parallel connected microbial fuel cells 模拟外部负载变化对单个、串联和并联微生物燃料电池的影响。

IF 9.7 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology

Pub Date : 2024-11-06 DOI: 10.1016/j.biortech.2024.131761

S. Potrykus , J. Nieznański , F. Kutt , F.J. Fernandez-Morales

This paper presents a microbial fuel cell (MFC) model designed to analyze the effect of the external load on MFC performance. The model takes into account the voltage and the chemical oxygen demand (COD) dependence on the external load. The value of the model parameters were calibrated by means of the voltage relaxation method tests using a controlled load current. Laboratory measurements and MATLAB Simulink model computations were used to validate the proposed model. The tests results demonstrated that the proposed model accurately predicts the voltage and COD evolution during the batch cycle of the MFC. The root mean square error (RMSE) was used to assess the fitting goodness of the model. The RMSE of COD and voltage generation was in all the cases lower than 4%, predicting accurately the behaviour of single MFC as well as MFC connected in series or parallel.

本文介绍了一种微生物燃料电池（MFC）模型，旨在分析外部负载对 MFC 性能的影响。该模型考虑了电压和化学需氧量（COD）对外部负载的依赖性。模型参数值通过使用受控负载电流的电压松弛法测试进行校准。实验室测量和 MATLAB Simulink 模型计算用于验证所提出的模型。测试结果表明，所提出的模型能够准确预测 MFC 批次循环期间的电压和 COD 变化。均方根误差（RMSE）用于评估模型的拟合程度。在所有情况下，化学需氧量和电压生成的均方根误差都低于 4%，能够准确预测单个 MFC 以及串联或并联 MFC 的行为。

引用次数: 0

Potential role of N-acyl homoserine lactone-mediated quorum sensing in the adaptation of anammox granular sludge system to salinity stress N-酰基高丝氨酸内酯介导的法定量传感在氨氧化颗粒污泥系统适应盐度胁迫中的潜在作用。

IF 9.7 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology

Pub Date : 2024-11-06 DOI: 10.1016/j.biortech.2024.131758

Peng Gao , Andong Zhao , Xin Zhang , Peng Tang , Dongyue Li , Tingting Liu , Jun Li , Yuhan Zhu , Zhaozhao Wang

Anammox granular sludge (AnGS) systems efficiently remove nitrogen from saline wastewater, but their adaptation mechanisms to salt stress are unclear. This study explores the adaptability of the AnGS system when exposed to salinity (0–30 g NaCl/L), focusing on the role of N-acyl homoserine lactone-mediated quorum sensing (AHL-QS) in microbial responses and community symbiosis under stress. Based on Hill model assessments, AnGS tolerates salt stress up to 15.73 g/L. Within this range, AnGS maintains cellular stability by enhancing extracellular polymeric substances (EPS) release, regulating oxidative stress; and drives nitrogen metabolism by increasing cytochrome c-activity to maintain electron transfer. With the mantel test and validation experiments, salt stimulates QS, leading to increased AHL (C6-HSL and C8-HSL) secretion associated with EPS release, extracellular electron transfer, and oxidative stress. Stabilization of AHL-QS genera supports AHL secretion and microbial symbiosis, promoting AnGS adaptation to salt stress. These insights facilitate optimizing AnGS for saline wastewater treatment.

Anammox 颗粒污泥（AnGS）系统能有效去除含盐废水中的氮，但其对盐胁迫的适应机制尚不清楚。本研究探讨了 AnGS 系统暴露于盐度（0-30 克 NaCl/升）时的适应性，重点是 N-酰基高丝氨酸内酯介导的法定量传感（AHL-QS）在压力下的微生物反应和群落共生中的作用。根据希尔模型评估，AnGS对盐胁迫的耐受力可达15.73克/升。在此范围内，AnGS 通过增强胞外聚合物物质（EPS）的释放来维持细胞的稳定性，调节氧化应激；并通过提高细胞色素 c 的活性来维持电子传递，从而推动氮代谢。通过套管试验和验证实验，盐刺激 QS，导致与 EPS 释放、细胞外电子传递和氧化应激相关的 AHL（C6-HSL 和 C8-HSL）分泌增加。AHL-QS 属的稳定支持 AHL 分泌和微生物共生，促进 AnGS 对盐胁迫的适应。这些见解有助于优化含盐废水处理中的 AnGS。

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引用次数: 0

Evaluating sustainability of CO2-mediated pyrolysis of lignocellulose 评估以二氧化碳为媒介的木质纤维素热解的可持续性。

IF 9.7 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology

Pub Date : 2024-11-06 DOI: 10.1016/j.biortech.2024.131765

Kwangsuk Yoon , Taewoo Lee , Hoyeon Cha , Joohyung Lee , Jegeon Lee , Hocheol Song

Despite the growing interest in biomass as a carbon–neutral resource, technical challenges have limited its comprehensive utilization. Pyrolysis has emerged as a promising method for reducing the carbon footprint by more effectively valorizing carbon in biomass. This study investigated the use of carbon dioxide (CO₂) in the pyrolysis of pine cone (PC), a lignocellulosic biomass. Thermogravimetric analysis confirmed that lignin was the primary component of the PC. Characterization and quantification of the three pyrolytic products (syngas, biocrude, and biochar) revealed that CO₂ enhanced CO production and the surface area of the biochar, thereby improving its CO₂ adsorption capacity. Additional heat and a Ni catalyst further amplified CO₂′s functionality. The sustainability of the proposed pyrolysis system was evaluated by calculating energy requirements of the pyrolysis processes and the net CO₂ emissions. Catalytic pyrolysis under CO₂ was the most effective, achieving a reduction of 3.34 g of CO₂ per gram of PC.

尽管人们对生物质这种碳中和资源的兴趣与日俱增，但技术上的挑战限制了生物质的综合利用。通过更有效地利用生物质中的碳，热解已成为减少碳足迹的一种有前途的方法。本研究调查了二氧化碳（CO2）在木质纤维素生物质松果（PC）热解过程中的应用。热重分析证实木质素是 PC 的主要成分。对三种热解产物（合成气、生物原油和生物炭）进行表征和定量分析后发现，二氧化碳提高了二氧化碳的产生量和生物炭的表面积，从而提高了生物炭对二氧化碳的吸附能力。额外的热量和镍催化剂进一步增强了二氧化碳的功能。通过计算热解过程的能源需求和二氧化碳净排放量，评估了拟议热解系统的可持续性。二氧化碳催化热解最为有效，每克 PC 可减少 3.34 克二氧化碳。

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引用次数: 0

Aerobic granular sludge enhances start-up and granulation in single-stage partial nitritation anammox granular sludge systems: Performance, mechanism, and shifts in bacterial communities 好氧颗粒污泥可增强单级部分亚硝酸盐化厌氧发酵颗粒污泥系统的启动和颗粒化：性能、机理和细菌群落的变化。

IF 9.7 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology

Pub Date : 2024-11-06 DOI: 10.1016/j.biortech.2024.131760

Jing Huang , Jun Li , Xiaoyu Han , Zedong Lu , Shujun Zhang , Zehao Zhang

The rapid start-up and granulation of a single-stage partial nitritation anammox granular sludge (PN/AnGS) system under limited seed sludge conditions is crucial for its practical application. This study proposed an aerobic granular sludge (AGS) − based strategy, enhanced the enrichment of anammox bacteria (AnAOB), and shortened the start-up time of PN/AnGS system by 20.5%. In addition, the inoculation of AGS can ensure the stable operation of the system during the selective sludge discharge to washout the flocs. Microbial community structure, particle size distribution, morphology results showed that niche shift was the key to promote the enrichment of AnAOB, and AGS played a decisive role in the particle characteristics of PN/AnGS. Since AGS can be directly obtained from full-scale AGS wastewater treatment plants, integrating PN/AnGS with AGS processes can transition wastewater treatment from a “linear economy” to a “circular economy”, enhancing nitrogen removal efficiency and delivering significant economic and environmental benefits.

在有限的种子污泥条件下，单级部分亚硝化厌氧颗粒污泥（PN/AnGS）系统的快速启动和颗粒化对其实际应用至关重要。本研究提出了一种基于好氧颗粒污泥（AGS）的策略，提高了厌氧菌（AnAOB）的富集度，并将 PN/AnGS 系统的启动时间缩短了 20.5%。此外，接种 AGS 还能确保系统在选择性污泥排放冲刷絮凝物时稳定运行。微生物群落结构、粒径分布和形态学结果表明，生态位转移是促进 AnAOB 富集的关键，而 AGS 对 PN/AnGS 的颗粒特征起着决定性作用。由于 AGS 可直接从全套 AGS 污水处理厂中获得，因此将 PN/AnGS 与 AGS 工艺相结合，可将污水处理从 "线性经济 "过渡到 "循环经济"，提高脱氮效率，并带来显著的经济和环境效益。

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引用次数: 0

Engineering thermotolerant Kluyveromyces marxianus strains for resveratrol production by simultaneous saccharification and fermentation of whole slurry corn cob 通过同时糖化和发酵全浆玉米芯，培养耐高温的 Kluyveromyces marxianus 菌株以生产白藜芦醇。

IF 9.7 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology

Pub Date : 2024-11-05 DOI: 10.1016/j.biortech.2024.131755

Marlene Baptista , Carlos E. Costa , Lucília Domingues

The microbial biosynthesis of the antioxidant compound resveratrol offers an eco-friendly and sustainable alternative to chemical synthesis or plant extraction. Here, we showed that Kluyveromyces marxianus strains produce p-coumaric acid, a key precursor of resveratrol, with higher titres achieved under increased agitation conditions. Through further strain engineering, resveratrol production was achieved using glucose, xylose, and/or ethanol as substrates. Xylose emerged as the most favourable carbon source for resveratrol production, with ethanol supplementation during xylose culture resulting in increased resveratrol titres by limiting the accumulation of the by-product xylitol. At 37 ◦C, resveratrol production from corn cob hydrolysate and whole slurry substantially increased the yield of resveratrol per sugar, reaching titres of up to 69.26 mg/L. This work shows, for the first time, resveratrol production by K. marxianus and from corn cob whole slurry, establishing foundations for the development of an integrated sustainable process for resveratrol production from lignocellulosic materials.

抗氧化化合物白藜芦醇的微生物生物合成为化学合成或植物提取提供了一种生态友好和可持续的替代方法。在这里，我们发现马尔仙酵母菌（Kluyveromyces marxianus）菌株能产生对香豆酸（白藜芦醇的一种关键前体），并在增加搅拌条件下获得更高的滴度。通过进一步的菌株工程，以葡萄糖、木糖和/或乙醇为底物的白藜芦醇生产得以实现。木糖是生产白藜芦醇最有利的碳源，在木糖培养过程中补充乙醇可限制副产物木糖醇的积累，从而提高白藜芦醇滴度。在 37 ◦C 温度条件下，利用玉米芯水解物和全浆生产白藜芦醇可大幅提高每糖白藜芦醇的产量，滴度最高可达 69.26 毫克/升。这项研究首次展示了 K. marxianus 和玉米芯浆液生产白藜芦醇的过程，为开发一种利用木质纤维素材料生产白藜芦醇的综合可持续工艺奠定了基础。

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引用次数: 0

In-depth recognition of mixed surfactants maintaining the enzymatic activity of cellulases through stabilization of their spatial structures 深入认识混合表面活性剂通过稳定空间结构来保持纤维素酶的酶活性。

IF 9.7 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology

Pub Date : 2024-11-05 DOI: 10.1016/j.biortech.2024.131756

Qiangqiang Liu , Meysam Madadi , Salauddin Al Azad , Chihe Sun , Ezhen Zhang , Junshu Yan , Abdolreza Samimi , Fubao Sun

Mixed surfactants improve the enzymatic hydrolysis of lignocellulosic substrates by enhancing cellulase stability against heat, pH, shear, and air–liquid interface stress. Under conditions of multiple factorial stresses (50 °C, pH 4.8, 180 rpm, and 15.5 cm² air–liquid interface), cellulase with ternary surfactants (Tween 60/Triton X-114/CTAB, the molar ratio 14:5.5:1) retained 84 % of its activity after 48 h of incubation, representing 1.15 and 1.29 folds that of the cellulase activity with the single Tween 60 and with no surfactants, respectively. This is attributed to the fact that ternary surfactants possess better rheology modulation and air–liquid interface competitiveness. In addition, the computational approach demonstrated that the ternary surfactants were capable of forming stronger hydrophobic and hydrogen-bond interactions with cellulase enzymes, thus maintaining its secondary structure and preventing the detrimental α-helix to β-sheet transformation known to compromise cellulase activity. This synergy offers valuable insights into surfactant-cellulase interactions and supports efficient enzymatic hydrolysis in biorefineries.

混合表面活性剂通过提高纤维素酶对热、pH 值、剪切力和气液界面应力的稳定性来改善木质纤维素底物的酶水解。在多重因子应力（50 °C、pH 4.8、180 rpm 和 15.5 cm2 气液界面）条件下，添加三元表面活性剂（Tween 60/Triton X-114/CTAB，摩尔比为 14:5.5:1）的纤维素酶在培养 48 小时后保持了 84% 的活性，分别是添加单一 Tween 60 和不添加表面活性剂的纤维素酶活性的 1.15 倍和 1.29 倍。这是因为三元表面活性剂具有更好的流变调节性和气液界面竞争性。此外，计算方法还表明，三元表面活性剂能够与纤维素酶形成更强的疏水和氢键相互作用，从而保持其二级结构，防止已知会损害纤维素酶活性的有害的 α 螺旋向 β 片状转变。这种协同作用为了解表面活性剂与纤维素酶之间的相互作用提供了宝贵的信息，并有助于生物炼油厂进行高效的酶水解。

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引用次数: 0

Preparation of a novel organic-inorganic composite sludge bioflocculant (SBF) from dewatered sludge as raw material: Characteristics, flocculation mechanism and application for domestic sewage 以脱水污泥为原料制备新型有机-无机复合污泥生物絮凝剂（SBF）：特性、絮凝机理及在生活污水中的应用。

IF 9.7 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology

Pub Date : 2024-11-05 DOI: 10.1016/j.biortech.2024.131747

Jingyun Ge , Hongyu Tian , Lin Li , Yunping Han , Jianwei Liu

In this work, a green sludge bioflocculant (SBF) was prepared via chemical hydrolysis of dewatered sludge and applied to flocculation of domestic wastewater. The process parameters for the preparation of the SBF were 1.80 % hydrochloric acid concentration, 60 min extraction time, and 4000 r/min centrifugation speed. SBF is polymeric flocculant composed of organic and inorganic compounds. Flocculation efficiency reached 97.31 ± 0.26 % under optimal flocculation conditions. Charge neutralization promotes the surface adsorption, bridging and net trapping and sweeping of Fe (OH)₃, Al (OH)₃ and active functional groups O–H/N–H and C = O in SBF, which together achieve efficient flocculation reactions. SBF had high efficiency and stable flocculation performance for phosphorus in urban domestic wastewater, and the concentration of TP in effluent was lower than 0.30 mg/L. Therefore, SBF prepared from dewatered sludge has efficient flocculation properties and is suitable for removing pollutant phosphorus, which has good application prospects in the field of wastewater treatment.

本研究通过化学水解脱水污泥制备了一种绿色污泥生物絮凝剂（SBF），并将其应用于生活污水的絮凝。制备 SBF 的工艺参数为盐酸浓度 1.80%、萃取时间 60 分钟、离心速度 4000 r/min。SBF 是由有机和无机化合物组成的高分子絮凝剂。在最佳絮凝条件下，絮凝效率达到 97.31 ± 0.26 %。电荷中和促进了SBF中Fe (OH)3、Al (OH)3以及活性官能团O-H/N-H和C = O的表面吸附、架桥和净捕集与扫除，共同实现了高效的絮凝反应。SBF 对城市生活污水中的磷具有高效、稳定的絮凝性能，出水 TP 浓度低于 0.30 mg/L。因此，利用脱水污泥制备的 SBF 具有高效的絮凝性能，适用于去除污染物磷，在污水处理领域具有良好的应用前景。

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引用次数: 0

re-aerobic treatment and dissolved oxygen regulation in full-scale aerobic-hydrolysis and denitrification-aerobic process for achieving simultaneous detoxification and nitrification of coking wastewater 全规模好氧-水解和反硝化-好氧工艺中的再好氧处理和溶解氧调节，以实现焦化废水的同步解毒和硝化。

IF 9.7 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology

Pub Date : 2024-11-05 DOI: 10.1016/j.biortech.2024.131754

Heng Zhang , Chaohai Wei , Acong Chen , Xiong Ke , Zemin Li , Zhi Qin , Yuxin Tian , Haizhen Wu , Guanglei Qiu , Shuang Zhu

The biological treatment of coking wastewater is a challenge. The application of prepositioned aerobic process has rarely been systematically reported, among which the detoxification and nitrification performance of the prepositioned aerobic unit (O1) is worthy of investigation. Results indicate that O1 achieves stable simultaneous detoxification and nitrification by regulating the dissolved oxygen, effectively maintaining ammonification, nitrosation, and complete nitrification phases. Microbial community structure, metabolic pathways and functional genes showed different preferences at different phases. High dissolved oxygen concentrations (2.20–3.00 mg/L) benefited the enrichment of carbon and nitrogen related major metabolic pathways and functional genes. BOD₅/COD_Cr ratio, dissolved oxygen and toxic pollutants together shaped microbial community structure and nitrogen transformation processes. Based on the principle of DO regulation, it could assemble a biotransformation compartment for nitrogen removal from complex wastewaters through a pollutant detoxification mechanism of rapid microbial proliferation，and provides a promising approach for toxic industrial wastewater.

焦化废水的生物处理是一项挑战。预置好氧工艺的应用鲜有系统报道，其中预置好氧单元（O1）的解毒和硝化性能值得研究。结果表明，O1 通过调节溶解氧实现了稳定的同步解毒和硝化，有效地维持了氨化、亚硝化和完全硝化阶段。微生物群落结构、代谢途径和功能基因在不同阶段表现出不同的偏好。高溶解氧浓度（2.20-3.00 mg/L）有利于与碳氮相关的主要代谢途径和功能基因的富集。BOD5/CODCr 比率、溶解氧和有毒污染物共同塑造了微生物群落结构和氮转化过程。基于溶解氧调控原理，它可以通过微生物快速增殖的污染物解毒机制，从复杂废水中建立一个脱氮生物转化区，为有毒工业废水的处理提供了一种可行的方法。

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引用次数: 0