Bioresource Technology最新文献

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Immobilization of ammonia-oxidizing bacteria using mycelial pellets: Preparation, characteristics, and application for nitritation

IF 11.4 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology

Pub Date : 2025-01-15 DOI: 10.1016/j.biortech.2025.132083

Ming Chen, Lei Jin, Xiaoying Liu, Renjie Li, Huiling Xian, Chao Guo

Ammonia-oxidizing bacteria (AOB) sourced from an aerobic granular sludge (AGS) process were rapidly enriched by progressively increasing ammonia nitrogen (NH4+-N) loads, achieving a Nitrosomonas abundance of 20.7 % and a nitrite accumulation rate exceeding 80 %. Mycelial pellets formed by Cladosporium, isolated from the same AGS system, provided a porous surface structure for the immobilization of the enriched AOB, creating mycelial pellet/AOB composites. Robust microbial colonization and aggregation in mycelial pellet porous matrix were facilitated by a higher level of extracellular polymeric substances (EPS) compared to conventional AGS. Static tests showed a maximum NH4+-N oxidation rate of 17.7 mg/(gMLVSS·h), higher than free AOB (8.5 mg/(gMLVSS·h)). In multi-recycling tests, the composites maintained 96.6 % NH4+-N oxidation, demonstrating superior repeatability and stability. The results highlight advantages of mycelial pellets as biocompatible carriers in immobilizing AOB sourced from the same system, offering insights into improved nitritation performance and durability, making them promising for practical wastewater treatment.

{"title":"Immobilization of ammonia-oxidizing bacteria using mycelial pellets: Preparation, characteristics, and application for nitritation","authors":"Ming Chen, Lei Jin, Xiaoying Liu, Renjie Li, Huiling Xian, Chao Guo","doi":"10.1016/j.biortech.2025.132083","DOIUrl":"https://doi.org/10.1016/j.biortech.2025.132083","url":null,"abstract":"Ammonia-oxidizing bacteria (AOB) sourced from an aerobic granular sludge (AGS) process were rapidly enriched by progressively increasing ammonia nitrogen (NH<ce:inf loc=\"post\">4</ce:inf><ce:sup loc=\"post\">+</ce:sup>-N) loads, achieving a <ce:italic>Nitrosomonas</ce:italic> abundance of 20.7 % and a nitrite accumulation rate exceeding 80 %. Mycelial pellets formed by <ce:italic>Cladosporium</ce:italic>, isolated from the same AGS system, provided a porous surface structure for the immobilization of the enriched AOB, creating mycelial pellet/AOB composites. Robust microbial colonization and aggregation in mycelial pellet porous matrix were facilitated by a higher level of extracellular polymeric substances (EPS) compared to conventional AGS. Static tests showed a maximum NH<ce:inf loc=\"post\">4</ce:inf><ce:sup loc=\"post\">+</ce:sup>-N oxidation rate of 17.7 mg/(gMLVSS·h), higher than free AOB (8.5 mg/(gMLVSS·h)). In multi-recycling tests, the composites maintained 96.6 % NH<ce:inf loc=\"post\">4</ce:inf><ce:sup loc=\"post\">+</ce:sup>-N oxidation, demonstrating superior repeatability and stability. The results highlight advantages of mycelial pellets as biocompatible carriers in immobilizing AOB sourced from the same system, offering insights into improved nitritation performance and durability, making them promising for practical wastewater treatment.","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"8 1","pages":"132083"},"PeriodicalIF":11.4,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142990135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Artificial cell-free system for the sustainable production of acetoin from bioethanol.

IF 9.7 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology

Pub Date : 2025-01-15 DOI: 10.1016/j.biortech.2025.132059

David Muñoz-Sánchez, Albert Carceller, Gregorio Álvaro, Óscar Romero, Marina Guillén

The present work introduces and validates an artificial cell free system for the synthesis of acetoin from ethanol, representing a greener alternative to conventional chemical synthesis. The one pot multi-enzymatic system, which employs pyruvate decarboxylase from Zymobacter palmae (ZpPDC), alcohol dehydrogenase from Saccharomyces cerevisiae (ScADH), and NADH oxidase from Streptococcus pyogenes (SpNOX), achieves nearly 100 % substrate conversion and reaction yield within 6 h under optimal conditions (pH 7.5, enzyme activities: ZpPDC 100 U·mL^-1, ScADH 50 U·mL^-1, SpNOX 127 U·mL^-1, and 1 mM NAD⁺). Using air for oxygen supply mitigates enzyme inactivation while effectively accelerating the regeneration of NAD⁺. The use of bioethanol as a substrate demonstrates the robustness and sustainability of the bioprocess, enabling the production of natural acetoin from renewable resources. This environmentally friendly approach offers significant advantages for industrial applications, aligning with green chemistry principles.

{"title":"Artificial cell-free system for the sustainable production of acetoin from bioethanol.","authors":"David Muñoz-Sánchez, Albert Carceller, Gregorio Álvaro, Óscar Romero, Marina Guillén","doi":"10.1016/j.biortech.2025.132059","DOIUrl":"https://doi.org/10.1016/j.biortech.2025.132059","url":null,"abstract":"The present work introduces and validates an artificial cell free system for the synthesis of acetoin from ethanol, representing a greener alternative to conventional chemical synthesis. The one pot multi-enzymatic system, which employs pyruvate decarboxylase from Zymobacter palmae (ZpPDC), alcohol dehydrogenase from Saccharomyces cerevisiae (ScADH), and NADH oxidase from Streptococcus pyogenes (SpNOX), achieves nearly 100 % substrate conversion and reaction yield within 6 h under optimal conditions (pH 7.5, enzyme activities: ZpPDC 100 U·mL-1, ScADH 50 U·mL-1, SpNOX 127 U·mL-1, and 1 mM NAD+). Using air for oxygen supply mitigates enzyme inactivation while effectively accelerating the regeneration of NAD+. The use of bioethanol as a substrate demonstrates the robustness and sustainability of the bioprocess, enabling the production of natural acetoin from renewable resources. This environmentally friendly approach offers significant advantages for industrial applications, aligning with green chemistry principles.","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"132059"},"PeriodicalIF":9.7,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142997215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbially degradable phenolic foams based on depolymerized Kraft lignin for hydrophilic applications

IF 11.4 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology

Pub Date : 2025-01-15 DOI: 10.1016/j.biortech.2025.132082

Glen Cletus DSouza, Chonlong Chio, Aditya Venkatesh, Haoyu Wang, Madhumita B. Ray, Anand Prakash, Wensheng Qin, Chunbao Xu

Hydrophilic phenol–formaldehyde (PF) foams, widely used in floral and hydroponic applications, are produced using phenol typically derived from non-renewable petroleum-based resources. This study examines the potential of depolymerized Kraft lignin (DKL) as a sustainable substitute for phenol in the synthesis of hydrophilic biobased foams. At 50 % DKL substitution, the foams demonstrated excellent water absorption capacities (up to 2557 %), relatively low densities (∼62 kg/m3), and nearly 100 % open-cell content. Its compressive strength (20.64 kPa at 10 % deformation) is comparable to commercially available floral and hydroponic foams. Additionally, foams with 10 % phenol substitution by DKL exhibited better thermal stability compared to neat phenolic foams. After 15 days of incubation with Laccase-producing bacterium Bacillus sp., 30 % and 50 % DKL foams exhibited the highest weight loss of 39.03 % and 38.9 %, respectively. Qualitative degree of biodegradation was further assessed using scanning electron microscopy and FT-IR analysis of the degraded samples.

{"title":"Microbially degradable phenolic foams based on depolymerized Kraft lignin for hydrophilic applications","authors":"Glen Cletus DSouza, Chonlong Chio, Aditya Venkatesh, Haoyu Wang, Madhumita B. Ray, Anand Prakash, Wensheng Qin, Chunbao Xu","doi":"10.1016/j.biortech.2025.132082","DOIUrl":"https://doi.org/10.1016/j.biortech.2025.132082","url":null,"abstract":"Hydrophilic phenol–formaldehyde (PF) foams, widely used in floral and hydroponic applications, are produced using phenol typically derived from non-renewable petroleum-based resources. This study examines the potential of depolymerized Kraft lignin (DKL) as a sustainable substitute for phenol in the synthesis of hydrophilic biobased foams. At 50 % DKL substitution, the foams demonstrated excellent water absorption capacities (up to 2557 %), relatively low densities (∼62 kg/m<ce:sup loc=\"post\">3</ce:sup>), and nearly 100 % open-cell content. Its compressive strength (20.64 kPa at 10 % deformation) is comparable to commercially available floral and hydroponic foams. Additionally, foams with 10 % phenol substitution by DKL exhibited better thermal stability compared to neat phenolic foams. After 15 days of incubation with Laccase-producing bacterium <ce:italic>Bacillus</ce:italic> sp., 30 % and 50 % DKL foams exhibited the highest weight loss of 39.03 % and 38.9 %, respectively. Qualitative degree of biodegradation was further assessed using scanning electron microscopy and FT-IR analysis of the degraded samples.","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"23 1","pages":""},"PeriodicalIF":11.4,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142988149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Air nanobubble simultaneously enhances hydrolysis and methane yield of sludge temperature phased-anaerobic digestion

IF 11.4 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology

Pub Date : 2025-01-15 DOI: 10.1016/j.biortech.2025.132084

Ziying Xu, Tianfeng Wang, Cheng Peng, Yutong Feng, Xin Fan, Xuan Yang, Wenqi Gao, Qingfang Zhang

Nanobubble water (NBW) or temperature-phased anaerobic digestion assisted by microbial electrolysis cell (MEC-TPAD) can promote sludge hydrolysis and methanogenesis. However, the role of the combined application of NBW and MEC-TPAD in terms of anaerobic performance and related microbial properties remains unclear. This study investigated the impact of Air-NBW on hydrolysis and methanogenesis of dewatered sludge MEC-TPAD. Under different temperatures, NBW increased ammonia nitrogen by 7.8%-13.7% in the hydrolysis phase and ultimate methane yield by 23.3%-41.5%. NBW can significantly promote hydrolysis under mesophilic-mesophilic conditions, while it can promote substantially methanogenesis under thermophilic-thermophilic conditions. Moreover, NBW increased the diversity and richness of microorganisms in hydrolysis. As to bacteria, NBW increased the relative abundance (RA) of Firmicutes but decreased the RA of Proteobacteria. As to archaea, NBW increased the RA of Methanosarcina in hydrolysis but decreased it in methanogenesis. NBW synchronized with MEC-TPAD improved hydrolysis and methanogenesis of the dewatered sludge digestion process.

{"title":"Air nanobubble simultaneously enhances hydrolysis and methane yield of sludge temperature phased-anaerobic digestion","authors":"Ziying Xu, Tianfeng Wang, Cheng Peng, Yutong Feng, Xin Fan, Xuan Yang, Wenqi Gao, Qingfang Zhang","doi":"10.1016/j.biortech.2025.132084","DOIUrl":"https://doi.org/10.1016/j.biortech.2025.132084","url":null,"abstract":"Nanobubble water (NBW) or temperature-phased anaerobic digestion assisted by microbial electrolysis cell (MEC-TPAD) can promote sludge hydrolysis and methanogenesis. However, the role of the combined application of NBW and MEC-TPAD in terms of anaerobic performance and related microbial properties remains unclear. This study investigated the impact of Air-NBW on hydrolysis and methanogenesis of dewatered sludge MEC-TPAD. Under different temperatures, NBW increased ammonia nitrogen by 7.8%-13.7% in the hydrolysis phase and ultimate methane yield by 23.3%-41.5%. NBW can significantly promote hydrolysis under mesophilic-mesophilic conditions, while it can promote substantially methanogenesis under thermophilic-thermophilic conditions. Moreover, NBW increased the diversity and richness of microorganisms in hydrolysis. As to bacteria, NBW increased the relative abundance (RA) of <ce:italic>Firmicutes</ce:italic> but decreased the RA of <ce:italic>Proteobacteria</ce:italic>. As to archaea, NBW increased the RA of <ce:italic>Methanosarcina</ce:italic> in hydrolysis but decreased it in methanogenesis. NBW synchronized with MEC-TPAD improved hydrolysis and methanogenesis of the dewatered sludge digestion process.","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"13 1","pages":"132084"},"PeriodicalIF":11.4,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142990134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of carrier capacitance on Geobacter enrichment and direct interspecies electron transfer under anaerobic conditions.

IF 9.7 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology

Pub Date : 2025-01-15 DOI: 10.1016/j.biortech.2025.132079

Shujuan Liu, Dandan Liang, Yixi Wang, Weihua He, Yujie Feng

Direct interspecies electron transfer (DIET) enhances anaerobic digestion by facilitating electron exchange between electroactive bacteria and methanogenic archaea. While Geobacter species are recognized for donating electrons to methanogens via DIET, they are rarely detected in mixed microbial communities. This study examined various non-electrode biological carriers (zeolite, carbon cloth, activated carbon and biochar) to promote Geobacter cultivation under anaerobic conditions and identify pivotal factors influencing their symbiosis with methanogens. Capacitive materials, such as activated carbon and biochar, significantly enriched Geobacter populations and strengthened DIET-based mutualism with Methanosarcina, both in the presence and absence of electric fields. Partial least-squares path modeling revealed that the porous structure and functional groups of materials positively and directly influenced the abundance of Geobacter and Methanosarcina. These findings contribute to a deeper understanding of critical properties of capacitive materials for screening functional microorganisms and guiding the design of electroactive materials to augment anaerobic treatment processes.

{"title":"Impact of carrier capacitance on Geobacter enrichment and direct interspecies electron transfer under anaerobic conditions.","authors":"Shujuan Liu, Dandan Liang, Yixi Wang, Weihua He, Yujie Feng","doi":"10.1016/j.biortech.2025.132079","DOIUrl":"https://doi.org/10.1016/j.biortech.2025.132079","url":null,"abstract":"Direct interspecies electron transfer (DIET) enhances anaerobic digestion by facilitating electron exchange between electroactive bacteria and methanogenic archaea. While Geobacter species are recognized for donating electrons to methanogens via DIET, they are rarely detected in mixed microbial communities. This study examined various non-electrode biological carriers (zeolite, carbon cloth, activated carbon and biochar) to promote Geobacter cultivation under anaerobic conditions and identify pivotal factors influencing their symbiosis with methanogens. Capacitive materials, such as activated carbon and biochar, significantly enriched Geobacter populations and strengthened DIET-based mutualism with Methanosarcina, both in the presence and absence of electric fields. Partial least-squares path modeling revealed that the porous structure and functional groups of materials positively and directly influenced the abundance of Geobacter and Methanosarcina. These findings contribute to a deeper understanding of critical properties of capacitive materials for screening functional microorganisms and guiding the design of electroactive materials to augment anaerobic treatment processes.","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"419 ","pages":"132079"},"PeriodicalIF":9.7,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142997210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synthetic microbial community enhances lignocellulose degradation during composting by assembling fungal communities

IF 11.4 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology

Pub Date : 2025-01-13 DOI: 10.1016/j.biortech.2025.132068

Qiumei Liu, Zhouling Xie, Siyu Tang, Qingquan Xie, Xunyang He, Dejun Li

Inoculating synthetic microbial community (SynCom) has been proposed as an eco-friendly approach for lignocellulose degradation in composting to enhance organic fertilizer quality. However, the mechanisms responsible for SynCom-regulated lignocellulose degradation during composting remain unclear. Here the SynCom inoculation decreased cellulose and hemicellulose contents by 26.2% and 14.3%, respectively, at the mature phase, while increasing endoglucanase, exoglucanase, and β-glucosidase activities significantly. SynCom inoculation increased the abundance of Cephaliophoras and Thermomyces at the mesophilic phase, Sordariomycetes at the thermophilic phase, and Thermomyces, Acremonium, Aspergillus, and Sordariomycetes at the mature phase, as well as increased the abundance of numerous Operational Taxonomic Units (OTUs), with OTU10 (Hydropisphaera) being responsible for lignocellulose degradation. The altered fungal community stimulated functions of the wood saprotroph, undefined saprotroph, and litter saprotroph were responsible for lignocellulose degradation via changing microbial community. The results suggest that SynCom inoculation effectively stimulate lignocellulose degradation, so that benefits quality improvement of organic fertilizer.

{"title":"Synthetic microbial community enhances lignocellulose degradation during composting by assembling fungal communities","authors":"Qiumei Liu, Zhouling Xie, Siyu Tang, Qingquan Xie, Xunyang He, Dejun Li","doi":"10.1016/j.biortech.2025.132068","DOIUrl":"https://doi.org/10.1016/j.biortech.2025.132068","url":null,"abstract":"Inoculating synthetic microbial community (SynCom) has been proposed as an eco-friendly approach for lignocellulose degradation in composting to enhance organic fertilizer quality. However, the mechanisms responsible for SynCom-regulated lignocellulose degradation during composting remain unclear. Here the SynCom inoculation decreased cellulose and hemicellulose contents by 26.2% and 14.3%, respectively, at the mature phase, while increasing endoglucanase, exoglucanase, and β-glucosidase activities significantly. SynCom inoculation increased the abundance of <ce:italic>Cephaliophoras</ce:italic> and <ce:italic>Thermomyces</ce:italic> at the mesophilic phase, <ce:italic>Sordariomycetes</ce:italic> at the thermophilic phase, and <ce:italic>Thermomyces</ce:italic>, <ce:italic>Acremonium</ce:italic>, <ce:italic>Aspergillus</ce:italic>, and <ce:italic>Sordariomycetes</ce:italic> at the mature phase, as well as increased the abundance of numerous Operational Taxonomic Units (OTUs), with OTU10 (<ce:italic>Hydropisphaera</ce:italic>) being responsible for lignocellulose degradation. The altered fungal community stimulated functions of the wood saprotroph, undefined saprotroph, and litter saprotroph were responsible for lignocellulose degradation via changing microbial community. The results suggest that SynCom inoculation effectively stimulate lignocellulose degradation, so that benefits quality improvement of organic fertilizer.","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"37 1","pages":""},"PeriodicalIF":11.4,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142988152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing the co-utilization of methanol and CO2 into 1-butanol by equipping synergistic reductive glycine pathway in Butyribacterium methylotrophicum

IF 11.4 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology

Pub Date : 2025-01-13 DOI: 10.1016/j.biortech.2025.132071

Jing Wang, Shengji Li, Chenxi Ma, Rui Zhang, Jialun Qin, Kequan Chen, Xin Wang

The biological fixation of CO2 and C1-feedstocks like methanol derived from CO2 are considered as an important technology combating in global warming issues. The microorganisms that can co-assimilate CO2 and methanol are highly desired. Here, we constructed a synergistic assimilation pathway in Butyribacterium methylotrophicum (B. methylotrophicum) for improved carbon utilization efficiency. Through a transcriptional analysis, the genes involving in the native methanol and CO2 assimilation pathway, oxidative phosphorylation and amino acid metabolism were significantly up-regulated, indicating the functional cooperation of the pathways in improving cell activity on methanol and CO2. Ultimately, by overexpressing exogenous genes of adhE2 in recombinant B. methylotrophicum, 1.4 g/L of 1-butanol was successfully synthesized from methanol and CO2, which was also the highest titer of 1-butanol synthesis using C1-feedstocks. Thus, the design of synergistic methanol assimilation pathway was an effective approach to improve the carbon assimilation capacity of strain for the establishment of C1-feedstock biotransformation platforms.

二氧化碳的生物固化和二氧化碳衍生甲醇等 C1 原料被认为是应对全球变暖问题的一项重要技术。能够同时同化二氧化碳和甲醇的微生物是非常需要的。在此，我们构建了甲基营养丁酸杆菌（B. methylotrophicum）的协同同化途径，以提高碳利用效率。通过转录分析，涉及原生甲醇和二氧化碳同化途径、氧化磷酸化和氨基酸代谢的基因显著上调，表明这些途径在提高细胞对甲醇和二氧化碳的活性方面存在功能性合作。最终，通过在重组养甲虫中过表达 adhE2 的外源基因，成功地利用甲醇和 CO2 合成了 1.4 g/L 的 1-丁醇，这也是利用 C1 原料合成 1-丁醇的最高滴度。因此，设计协同甲醇同化途径是提高菌株碳同化能力以建立 C1 原料生物转化平台的有效方法。

{"title":"Enhancing the co-utilization of methanol and CO2 into 1-butanol by equipping synergistic reductive glycine pathway in Butyribacterium methylotrophicum","authors":"Jing Wang, Shengji Li, Chenxi Ma, Rui Zhang, Jialun Qin, Kequan Chen, Xin Wang","doi":"10.1016/j.biortech.2025.132071","DOIUrl":"https://doi.org/10.1016/j.biortech.2025.132071","url":null,"abstract":"The biological fixation of CO<ce:inf loc=\"post\">2</ce:inf> and C1-feedstocks like methanol derived from CO<ce:inf loc=\"post\">2</ce:inf> are considered as an important technology combating in global warming issues. The microorganisms that can co-assimilate CO<ce:inf loc=\"post\">2</ce:inf> and methanol are highly desired. Here, we constructed a synergistic assimilation pathway in <ce:italic>Butyribacterium methylotrophicum</ce:italic> (<ce:italic>B. methylotrophicum</ce:italic>) for improved carbon utilization efficiency. Through a transcriptional analysis, the genes involving in the native methanol and CO<ce:inf loc=\"post\">2</ce:inf> assimilation pathway, oxidative phosphorylation and amino acid metabolism were significantly up-regulated, indicating the functional cooperation of the pathways in improving cell activity on methanol and CO<ce:inf loc=\"post\">2</ce:inf>. Ultimately, by overexpressing exogenous genes of <ce:italic>adhE2</ce:italic> in recombinant <ce:italic>B. methylotrophicum</ce:italic>, 1.4 g/L of 1-butanol was successfully synthesized from methanol and CO<ce:inf loc=\"post\">2</ce:inf>, which was also the highest titer of 1-butanol synthesis using C1-feedstocks. Thus, the design of synergistic methanol assimilation pathway was an effective approach to improve the carbon assimilation capacity of strain for the establishment of C1-feedstock biotransformation platforms.","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"29 1","pages":""},"PeriodicalIF":11.4,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142986899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Production of polyhydroxybutyrate with high cell density cultivation using thermophile Caldimonas thermodepolymerans

IF 11.4 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology

Pub Date : 2025-01-13 DOI: 10.1016/j.biortech.2025.132073

Jun Won Jang, In Yeub Hwang, Ok Kyung Lee, Eun Yeol Lee

This study investigates the production of polyhydroxybutyrate (PHB) using the thermophilic bacterium Caldimonas thermodepolymerans in fed-batch fermentation. This research highlights the potential of thermophilic bacteria in biopolymer production due to their ability to operate at high temperatures, which reduces contamination risks and enhances energy efficiency. Optimal fermentation conditions were identified at a temperature of 50 °C, with the strain achieving a maximum specific growth rate (μmax) of 0.57 h−1 and high biomass concentration of 63.1 gCDW/L. PHB production reached a peak concentration of 31.9 g/L with a productivity of 1.30 gPHB/L/h. The high cell density approach in fed-batch fermentation not only maximizes the productivity and yield of PHB, but also optimizes the production process, making it more suitable for industrial-scale applications. The findings highlight the potential of thermophilic bacteria as a sustainable solution for enhancing PHB production and advancing biodegradable polymer synthesis.

{"title":"Production of polyhydroxybutyrate with high cell density cultivation using thermophile Caldimonas thermodepolymerans","authors":"Jun Won Jang, In Yeub Hwang, Ok Kyung Lee, Eun Yeol Lee","doi":"10.1016/j.biortech.2025.132073","DOIUrl":"https://doi.org/10.1016/j.biortech.2025.132073","url":null,"abstract":"This study investigates the production of polyhydroxybutyrate (PHB) using the thermophilic bacterium <ce:italic>Caldimonas thermodepolymerans</ce:italic> in fed-batch fermentation. This research highlights the potential of thermophilic bacteria in biopolymer production due to their ability to operate at high temperatures, which reduces contamination risks and enhances energy efficiency. Optimal fermentation conditions were identified at a temperature of 50 °C, with the strain achieving a maximum specific growth rate (μ<ce:inf loc=\"post\">max</ce:inf>) of 0.57 h<ce:sup loc=\"post\">−1</ce:sup> and high biomass concentration of 63.1 g<ce:inf loc=\"post\">CDW</ce:inf>/L. PHB production reached a peak concentration of 31.9 g/L with a productivity of 1.30 g<ce:inf loc=\"post\">PHB</ce:inf>/L/h. The high cell density approach in fed-batch fermentation not only maximizes the productivity and yield of PHB, but also optimizes the production process, making it more suitable for industrial-scale applications. The findings highlight the potential of thermophilic bacteria as a sustainable solution for enhancing PHB production and advancing biodegradable polymer synthesis.","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"45 1","pages":""},"PeriodicalIF":11.4,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142988150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sustainable decentralized food waste composting using a pulse alternating ventilation pilot-scale device: Case study based on LCA and LCC analysis

IF 11.4 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology

Pub Date : 2025-01-13 DOI: 10.1016/j.biortech.2025.132078

Lin Zhu, Liyuan Liu, Chunxu Tan, Caokun Li, Boyi Le, Xiangwu Yao, Baolan Hu

Currently few efficient decentralized composting reactors have been developed, and there is also little exploration into their comprehensive environmental impact and carbon emissions. This study developed a continuous pulse alternating ventilation composting pilot device, SC-PAVCR. Results demonstrated that SC-PAVCR effectively maintained the thermophilic phase during the 120-day operation period. The organic matter degradation degree reached 44.05 %, and the humic acid content increased to 91.21 g·kg−1, accounting for 0.53 of the total organic carbon. Life cycle assessment analysis with windrow composting and machine composting revealed that SC-PAVCR reduced the normalized comprehensive environmental impact by 49 % and 25 %, respectively. The carbon emission intensity of SC-PAVCR was 44.3 kg CO2 eq per tonne of food waste, representing a reduction of 26 % and 48 % compared to the other two technologies. The economic cost of $1.91–3.98/FU was reduced. These findings provide technical guidance for the development of low-carbon food waste composting technologies.

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引用次数: 0

Changes in methanogenic performance and microbial community during gradual transition from co-digestion with food waste to mono-digestion of rice straw

IF 11.4 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology

Pub Date : 2025-01-13 DOI: 10.1016/j.biortech.2025.132072

Yaqian Liu, Ryoya Watanabe, Qian Li, Yutong Luo, Naohito Tsuzuki, Yu Qin, Yu-You Li

This study investigated the performance and phase-specific characteristics of mesophilic co-digestion of food waste (FW) with rice straw (RS) at different RS proportions (40 %, 60 %, and 80 %), as well as mono-digestion of RS. The system achieved optimal performance at 40 % RS content, with a methane yield of 383.8 mL/g-VS and cellulose removal efficiency exceeding 75 %. When RS content increased beyond 60 %, process performance declined notably. At 80 % RS ratio, an imbalance between acidogenesis and methanogenesis led to volatile fatty acids (VFAs) accumulation (1500 mg-COD/L), compromising both efficiency and stability. Notably, during the transition to mono-digestion, the system maintained stable operation, characterized by increased abundance of functional enzymes associated with cellulosic substance hydrolysis and acidogenesis. This enhanced enzymatic activity may be attributed to the microbial adaptation induced by previous co-digestion with FW. These findings provide valuable insights for optimizing mesophilic RS digestion through strategic co-digestion approaches.

{"title":"Changes in methanogenic performance and microbial community during gradual transition from co-digestion with food waste to mono-digestion of rice straw","authors":"Yaqian Liu, Ryoya Watanabe, Qian Li, Yutong Luo, Naohito Tsuzuki, Yu Qin, Yu-You Li","doi":"10.1016/j.biortech.2025.132072","DOIUrl":"https://doi.org/10.1016/j.biortech.2025.132072","url":null,"abstract":"This study investigated the performance and phase-specific characteristics of mesophilic co-digestion of food waste (FW) with rice straw (RS) at different RS proportions (40 %, 60 %, and 80 %), as well as mono-digestion of RS. The system achieved optimal performance at 40 % RS content, with a methane yield of 383.8 mL/g-VS and cellulose removal efficiency exceeding 75 %. When RS content increased beyond 60 %, process performance declined notably. At 80 % RS ratio, an imbalance between acidogenesis and methanogenesis led to volatile fatty acids (VFAs) accumulation (1500 mg-COD/L), compromising both efficiency and stability. Notably, during the transition to mono-digestion, the system maintained stable operation, characterized by increased abundance of functional enzymes associated with cellulosic substance hydrolysis and acidogenesis. This enhanced enzymatic activity may be attributed to the microbial adaptation induced by previous co-digestion with FW. These findings provide valuable insights for optimizing mesophilic RS digestion through strategic co-digestion approaches.","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"45 1","pages":""},"PeriodicalIF":11.4,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142988151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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