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Efficient synthesis of syringyl alcohol through bioreduction of lignin-derived syringaldehyde by newly constructed recombinant Escherichia coli C165F-V231D 重组大肠杆菌C165F-V231D生物还原木质素基丁香醛高效合成丁香醇
IF 11.4 1区 环境科学与生态学 Q1 AGRICULTURAL ENGINEERING Pub Date : 2026-02-09 DOI: 10.1016/j.biortech.2026.134193
Xin Li, Haoyu Chai, Zhifan Yang, Cuiluan Ma, Yu-Cai He
Syringyl alcohol is an important lignin-derived aromatic alcohol with potential utility in the fragrance and pharmaceutical research. In this study, it was biologically synthesized from syringaldehyde. The recombinant Escherichia coli C165F-V231D expressing alcohol dehydrogenase KpADH from Kluyveromyces polyspora enabled the effective biotransformation of syringaldehyde. Based on structural analysis and previous work for mutation of 165C to 165F and 231 V to 231D in KpADH. This variant was created, demonstrating a markedly increased catalytic activity under low dimethyl sulfoxide (DMSO) concentrations. C165F-V231D activity exhibited a 1.9-fold improvement in DMSO-H2O (5:95, V/V). Eventually, C165F-V231D cells transformed syringaldehyde (25 mM) to syringyl alcohol in 99.7% analytical yield. This work provides a sustainable biocatalytic strategy for the synthesis of syringyl alcohol from lignin-derived aldehydes, deserves the further development lignin valorization and the green synthesis of bio-derived aromatic alcohols.
丁香醇是一种重要的木质素衍生芳香醇,在香料和药物研究中具有潜在的应用价值。本研究以丁香醛为原料进行生物合成。重组大肠杆菌C165F-V231D表达多孢克卢维菌的醇脱氢酶KpADH,实现了丁香醛的有效生物转化。基于对KpADH中165C - 165F和231 V - 231D突变的结构分析和前人的工作。这种变体被创造出来,在低二甲亚砜(DMSO)浓度下显示出显著增加的催化活性。C165F-V231D在DMSO-H2O中的活性提高了1.9倍(5:95,V/V)。最终,C165F-V231D细胞以99.7%的分析产率将丁香醛(25 mM)转化为丁香醇。本研究为木质素衍生醛合成紫丁香醇提供了一种可持续的生物催化策略,值得进一步发展木质素增值和绿色合成生物衍生芳香醇。
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引用次数: 0
Unveiling the mechanisms of mechanical stirring for enhanced performance and stability of algal-bacterial flocs treating low C/N synthetic wastewater 揭示了机械搅拌提高藻菌絮凝体处理低碳氮比合成废水性能和稳定性的机理
IF 11.4 1区 环境科学与生态学 Q1 AGRICULTURAL ENGINEERING Pub Date : 2026-02-09 DOI: 10.1016/j.biortech.2026.134175
Jun-Jie Gu, Bin-Di Mao, Xiao-Xiao Dou, Bin-Xin Zhang, Jia-Wei Xu, Chun-Wan Fu, Bang-Jie Lan, Xin-Jie Zhang, Zhe Xu, Feng Gao
Algal-bacterial symbiotic systems (ABS) represent an environmentally sustainable wastewater treatment technology with significant application potential, though achieving stable and efficient operation remains a critical research challenge. This 180-day comparative study systematically investigated the performance differences and underlying mechanisms between mechanically stirred and aerated algal-bacterial symbiotic flocs (ABF) cultured in low C/N ratio wastewater. The results demonstrate that mechanical stirring enhances symbiotic interactions between microalgae and bacteria, leading to significantly improved performance metrics including higher biomass concentration (3.5 g/L), elevated dissolved oxygen levels (10.3 mg/L), increased lipid content (58.4%) and lipid productivity (9.3 mg/L/d), along with superior settling characteristics as evidenced by the reduced sludge volume index (80.7 mL/g). During Phase Ⅳ, the stirred ABFs exhibited exceptional contaminant removal efficiencies, achieving 98.2% ammonium nitrogen, 83.2% total nitrogen, and 89.7% chemical oxygen demand removal. Extracellular polymeric substance (EPS) analysis revealed stimulated secretion under stirring conditions (222.3 mg/g), with tight-bound EPS (TB-EPS) predominating, significantly enhancing floc structural stability. Metagenomic analysis demonstrated that stirring enriched functional genera like Thauera and Rubrivivax, strengthening denitrification and organic degradation capacities, while activating key pathways such as the TCA cycle and nitrogen metabolism, upregulating the abundance of EPS synthesis-related genes (e.g., galU), elucidating the molecular mechanisms underlying efficient nutrient removal and floc stability. This study presents an optimized strategy for establishing high-performance ABS in low C/N ratio wastewater treatment, offering both environmental sustainability and economic viability.
藻-细菌共生系统(ABS)是一种具有环境可持续性的污水处理技术,具有巨大的应用潜力,但如何实现稳定高效的运行仍是一个关键的研究挑战。这项为期180天的比较研究系统地研究了在低碳氮比废水中培养的机械搅拌和曝气藻菌共生絮凝体(ABF)的性能差异及其机制。结果表明,机械搅拌增强了微藻与细菌之间的共生相互作用,显著改善了性能指标,包括更高的生物量浓度(3.5 g/L)、更高的溶解氧水平(10.3 mg/L)、更高的脂质含量(58.4%)和脂质生产率(9.3 mg/L/d),以及污泥体积指数(80.7 mL/g)的降低所证明的卓越沉降特性。在Ⅳ阶段,搅拌ABFs表现出优异的污染物去除率,铵态氮去除率为98.2%,总氮去除率为83.2%,化学需氧量去除率为89.7%。细胞外聚合物(EPS)分析显示,在搅拌条件下刺激分泌(222.3 mg/g),以紧密结合的EPS (TB-EPS)为主,显著增强了絮凝体结构的稳定性。宏基因组分析表明,搅拌可以丰富Thauera和Rubrivivax等功能属,增强其反硝化和有机降解能力,同时激活TCA循环和氮代谢等关键途径,上调EPS合成相关基因(如galU)的丰度,从而阐明了有效去除营养物质和絮凝体稳定性的分子机制。本研究提出了在低碳氮比废水处理中建立高性能ABS的优化策略,同时提供了环境可持续性和经济可行性。
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引用次数: 0
Mechanistic insights into nitrogen doping effects on cobalt-loaded biochar for peroxymonosulfate Catalysis 氮掺杂对钴负载生物炭过氧单硫酸盐催化作用的机理研究
IF 11.4 1区 环境科学与生态学 Q1 AGRICULTURAL ENGINEERING Pub Date : 2026-02-09 DOI: 10.1016/j.biortech.2026.134179
Chuanbin Wang, Yixi Xie, Ning Li, Hailin Tian, Jiafeng Qu, Yanpeng Cai, Guanyi Chen, Qian Tan
Cow manure-derived biochar was used to synthesize a series of cobalt/nitrogen co-doped biochars with varying nitrogen contents for enhanced sulfamethoxazole (SMX) removal in a peroxymonosulfate (PMS)-based oxidation process. Their structures, catalytic activities, and mechanisms were systematically compared. Nitrogen doping significantly tuned biochar’s surface area, pore structure, electronic properties, and active site distribution, showing a “rise-then-fall” trend in catalytic performance. Medium nitrogen-doped biochar (CoBC-2) exhibited optimal PMS activation and SMX degradation effects, with higher reaction rates than low- or high-doped samples. Mechanistic analysis revealed that moderate nitrogen doping synergistically enhanced the activity of Co-O, graphitic N, and oxygen-containing functional groups. Density functional theory (DFT) calculations further confirmed that medium nitrogen-doped biochar promoted electron transfer, PMS adsorption, and OO bond cleavage, thereby efficiently generating reactive species. This work provides a clear guideline for designing tunable biochar catalysts with optimized nitrogen content.
利用牛粪生物炭合成了一系列不同氮含量的钴/氮共掺杂生物炭,用于在过氧单硫酸盐(PMS)氧化工艺中增强磺胺甲恶唑(SMX)的去除。系统比较了它们的结构、催化活性和机理。氮掺杂显著地改变了生物炭的表面积、孔隙结构、电子性质和活性位点分布,呈现出“先升后降”的催化性能趋势。中氮掺杂生物炭(CoBC-2)表现出最佳的PMS激活和SMX降解效果,其反应速率高于低掺杂和高掺杂样品。机制分析表明,适量的氮掺杂可以协同增强Co-O、石墨N和含氧官能团的活性。密度泛函理论(DFT)计算进一步证实,介质氮掺杂生物炭促进了电子转移、PMS吸附和OO键裂解,从而有效地产生了活性物质。这项工作为设计具有优化氮含量的生物炭催化剂提供了明确的指导。
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引用次数: 0
Cross-scale modeling of bacteria-contaminant spatiotemporal dynamics in 3D bioprinted hydrogel for dye biodegradation 生物3D打印水凝胶染料生物降解中细菌污染物时空动力学的跨尺度建模
IF 11.4 1区 环境科学与生态学 Q1 AGRICULTURAL ENGINEERING Pub Date : 2026-02-09 DOI: 10.1016/j.biortech.2026.134171
Weihao Guo, Ya-Nan Hou, Wei Xing, Ran Xu, Jinfeng Ma, Ai-Jie Wang, Nanqi Ren, Cong Huang
Three-dimensional (3D) bioprinting enables precise construction of functional biohydrogels, yet effective simulation bacterial dynamics within these structures remains challenging. Here, we developed a novel gelatin/cellulose/sodium alginate (GCSA) biohydrogel incorporating Shewanella oneidensis MR-1 with superior mechanical properties and biocompatibility. Using Direct Blue 71 (DB71) as a model contaminant, we demonstrated efficient bioremediation while elucidating protective mechanisms through comprehensive experimental characterization. We established a cross-scale “hydrogel-bacteria-digital model” framework integrating high-quality genome-scale metabolic model (GEM) with Computation Of Microbial Ecosystems in Time and Space (COMETS) simulation to bridge bacterial growth distribution and contaminant diffusion within biohydrogel microenvironments. This approach revealed fundamental mechanisms governing bacteria-pollutant interactions across multiple scales, validated optimal porous architecture for enhanced mass transfer, and demonstrated that biohydrogel encapsulation reduces bacterial oxidative stress while promoting metabolic activity. The framework exhibits flexibility and extensibility in addressing complex environmental challenges while advancing fundamental understanding of cross-scale interactions in engineered biological systems.
三维(3D)生物打印能够精确构建功能性生物水凝胶,但在这些结构中有效模拟细菌动力学仍然具有挑战性。在此,我们开发了一种新型明胶/纤维素/海藻酸钠(GCSA)生物水凝胶,其中含有希瓦氏菌MR-1,具有优异的力学性能和生物相容性。以直接蓝71 (DB71)为模型污染物,我们展示了有效的生物修复,同时通过全面的实验表征阐明了保护机制。我们建立了一个跨尺度的“水凝胶-细菌-数字模型”框架,将高质量的基因组尺度代谢模型(GEM)与微生物生态系统时空计算(COMETS)模拟相结合,以桥接生物水凝胶微环境中的细菌生长分布和污染物扩散。该方法揭示了跨多个尺度控制细菌-污染物相互作用的基本机制,验证了增强传质的最佳多孔结构,并证明了生物水凝胶包封可以减少细菌氧化应激,同时促进代谢活性。该框架在解决复杂的环境挑战方面表现出灵活性和可扩展性,同时推进了对工程生物系统中跨尺度相互作用的基本理解。
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引用次数: 0
Engineering stress tolerance in Saccharomyces cerevisiae by overexpressing PIR3 and SPI1 for efficient ethanol production from high-concentration sugarcane molasses 通过过表达PIR3和SPI1,从高浓度甘蔗糖蜜中高效生产乙醇,来改造酿酒酵母的抗逆性
IF 11.4 1区 环境科学与生态学 Q1 AGRICULTURAL ENGINEERING Pub Date : 2026-02-09 DOI: 10.1016/j.biortech.2026.134191
Wei-Yang Wang, Bei Liao, Ping Zheng, Ming-Yue Huang, Yu-Tuo Wei, Fu-Xing Niu
The coexistence of potassium and calcium ions has been identified as a major factor limiting high-yield ethanol fermentation by Saccharomyces cerevisiae in high-concentration sugarcane molasses. To identify key genes conferring tolerance to this stress, we employed an integrated strategy combining multi-omics analysis, CRISPR-mediated gene activation/repression, and targeted overexpression. This approach pinpointed four critical genes: PIR3, SPI1, AQR1, and GUT2. Functional analysis showed that while AQR1 and GUT2 enhance ethanol biosynthesis primarily by redirecting metabolic flux, PIR3 and SPI1 are crucial for maintaining cellular integrity and viability under stress. Overexpression of PIR3 and SPI1 in a wild-type strain increased ethanol production by 24.6%, achieving a final titer of 113.3 g/L in a 5-L fermenter, a performance comparable to robust industrial strains. Furthermore, this engineering strategy boosted the synthesis of other valuable compounds, exemplified by a 12.5% increase in cinnamic acid production. Our work thus identifies precise genetic targets for engineering stress-tolerant yeast and establishes a foundation for efficient bioconversion of high-concentration sugarcane molasses.
钾离子和钙离子的共存是制约酿酒酵母在高浓度甘蔗糖蜜中高产乙醇发酵的主要因素。为了确定对这种胁迫具有耐受性的关键基因,我们采用了一种综合策略,结合多组学分析、crispr介导的基因激活/抑制和靶向过表达。这种方法确定了四个关键基因:PIR3、SPI1、AQR1和GUT2。功能分析表明,虽然AQR1和GUT2主要通过重定向代谢通量来促进乙醇生物合成,但PIR3和SPI1在逆境下维持细胞完整性和活力至关重要。PIR3和SPI1在野生型菌株中过表达可使乙醇产量提高24.6%,在5-L发酵罐中最终滴度达到113.3 g/L,性能与强劲的工业菌株相当。此外,这种工程策略促进了其他有价值化合物的合成,例如肉桂酸的产量增加了12.5%。因此,我们的工作确定了工程抗逆性酵母的精确遗传靶点,并为高浓度甘蔗糖蜜的高效生物转化奠定了基础。
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引用次数: 0
Trichoderma-driven shifts in microbial communities improve spent mushroom substrate composting and disease-suppressive capacity 木霉驱动的微生物群落变化提高了废蘑菇基质堆肥和疾病抑制能力
IF 11.4 1区 环境科学与生态学 Q1 AGRICULTURAL ENGINEERING Pub Date : 2026-02-09 DOI: 10.1016/j.biortech.2026.134188
Hao Tan, Xia Kang, Qi Yin, Kexin Meng, Huizhu Yang, Linjing Ma, Weiwei Long, Xiang Wu, Zuopeng Lv
This study evaluated the role of an isolated strain, Trichoderma sp. BZ01A, in enhancing the composting of Flammulina filiformis spent mushroom substrate (SMS) for producing bio-organic fertilizer. The objective was to determine how inoculation influences microbial community dynamics, compost properties, and the resulting biocontrol efficacy against cucumber-wilt disease. Amendment with Trichoderma sp. BZ01A significantly altered the compost environment, reshaped microbial communities by reducing the relative abundance of fungal pathogens such as Fusarium (from 15% to < 1%) and Aspergillus, while increasing the inoculated Trichoderma to over 95% dominance. Community assembly analysis indicated a shift towards stochastic processes, and co-occurrence network analysis revealed a simplified fungal community with higher modularity and reduced connectivity for Fusarium. These microbial changes were associated with significant improvements in the final compost product. The germination index increased significantly (P < 0.05), and pot trials demonstrated that application of the Trichoderma-amended compost significantly reduced the incidence of cucumber-wilt disease by over 50% compared to the control, whereas the uninoculated compost exacerbated disease. Statistical analyses (PLS-PM) indicated that the fungal community composition was the primary factor influencing disease suppression. The results demonstrate that inoculating Trichoderma sp. BZ01A during SMS composting effectively steers the microbial community toward a suppressive state, leading to a high-quality compost with significant biocontrol potential against a major soil-borne disease.
本研究评价了分离菌株木霉sp. BZ01A在促进金针菇废菌基质(金针菇废菌基质)堆肥生产生物有机肥中的作用。目的是确定接种如何影响微生物群落动态,堆肥特性,以及由此产生的黄瓜枯萎病的生物防治效果。添加木霉sp. BZ01A显著改变了堆肥环境,重塑了微生物群落,降低了镰刀菌(从15%降至 <; 1%)和曲霉等真菌病原体的相对丰度,同时将接种的木霉的优势度提高到95%以上。群落组装分析表明,赤霉病菌群落向随机过程转变,共现网络分析表明,赤霉病菌群落结构简化,模块化程度提高,连通性降低。这些微生物的变化与最终堆肥产品的显著改善有关。苗期萌发指数显著提高(P < 0.05),盆栽试验表明,施用木霉添加的堆肥与对照相比,显著降低了黄瓜枯萎病的发病率,降幅超过50%,而未接种的堆肥则加重了病害。统计分析(PLS-PM)表明真菌群落组成是影响病害抑制的主要因素。结果表明,在SMS堆肥过程中接种木霉sp. BZ01A可有效引导微生物群落进入抑制状态,从而获得具有显著生物防治潜力的高质量堆肥。
{"title":"Trichoderma-driven shifts in microbial communities improve spent mushroom substrate composting and disease-suppressive capacity","authors":"Hao Tan, Xia Kang, Qi Yin, Kexin Meng, Huizhu Yang, Linjing Ma, Weiwei Long, Xiang Wu, Zuopeng Lv","doi":"10.1016/j.biortech.2026.134188","DOIUrl":"https://doi.org/10.1016/j.biortech.2026.134188","url":null,"abstract":"This study evaluated the role of an isolated strain, <ce:italic>Trichoderma</ce:italic> sp. BZ01A, in enhancing the composting of <ce:italic>Flammulina filiformis</ce:italic> spent mushroom substrate (SMS) for producing bio-organic fertilizer. The objective was to determine how inoculation influences microbial community dynamics, compost properties, and the resulting biocontrol efficacy against cucumber-wilt disease. Amendment with <ce:italic>Trichoderma</ce:italic> sp. BZ01A significantly altered the compost environment, reshaped microbial communities by reducing the relative abundance of fungal pathogens such as <ce:italic>Fusarium</ce:italic> (from 15% to &lt; 1%) and <ce:italic>Aspergillus</ce:italic>, while increasing the inoculated <ce:italic>Trichoderma</ce:italic> to over 95% dominance. Community assembly analysis indicated a shift towards stochastic processes, and co-occurrence network analysis revealed a simplified fungal community with higher modularity and reduced connectivity for <ce:italic>Fusarium</ce:italic>. These microbial changes were associated with significant improvements in the final compost product. The germination index increased significantly (P &lt; 0.05), and pot trials demonstrated that application of the <ce:italic>Trichoderma</ce:italic>-amended compost significantly reduced the incidence of cucumber-wilt disease by over 50% compared to the control, whereas the uninoculated compost exacerbated disease. Statistical analyses (PLS-PM) indicated that the fungal community composition was the primary factor influencing disease suppression. The results demonstrate that inoculating <ce:italic>Trichoderma</ce:italic> sp. BZ01A during SMS composting effectively steers the microbial community toward a suppressive state, leading to a high-quality compost with significant biocontrol potential against a major soil-borne disease.","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"30 1","pages":""},"PeriodicalIF":11.4,"publicationDate":"2026-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146146359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Dual-Functional Eggshell-Derived system for comprehensive phosphorus recovery from agricultural wastewater: From laboratory validation to Pilot-Scale implementation 从农业废水中全面回收磷的双重功能蛋壳衍生系统:从实验室验证到中试规模实施
IF 11.4 1区 环境科学与生态学 Q1 AGRICULTURAL ENGINEERING Pub Date : 2026-02-09 DOI: 10.1016/j.biortech.2026.134192
Qiao Wu, Maoqing Fan, Xiaoyu Zheng, Ming Zeng, Saihong Ru, Chengyou Sun, Chao Huang
The development of sustainable and economically feasible phosphorus recovery technology is of great significance to alleviate water eutrophication. This study proposed an innovative “one-waste, dual-use” strategy, involving the construction of a three-stage fixed-bed system integrating adsorption and Fenton-like oxidation. This system uses eggshell calcium-rich biochar (ESBC) as the phosphorus adsorbent and copper-functionalized eggshell membrane biochar (Cu@ESM) as the phosphorisation catalyst. The results showed that the integrated system has excellent treatment performance under weak alkaline conditions (pH 8–9). In the laboratory-scale treatment of high-concentration phosphorus-containing synthetic wastewater (400 mg P/L), the system was stable for approximately 940 min before reaching a 50% breakthrough. The pilot-scale test (100 L/h) was further validated with real pig wastewater, and it was confirmed that the system could continuously treat about 582 L of wastewater before saturation, and the operation was stable and efficient. Furthermore, by optimizing the molar ratio of calcium to phosphorus, high-purity hydroxyapatite was recovered from the concentrated eluent. This study presents a scalable technology based on the integration of agricultural waste valorization and advanced wastewater treatment, providing a promising solution for sustainable phosphorus management and recovery.
开发可持续、经济可行的磷回收技术对缓解水体富营养化具有重要意义。本研究提出了一种创新的“一废两用”策略,包括构建一个集吸附和Fenton-like氧化为一体的三级固定床系统。该系统采用蛋壳富钙生物炭(ESBC)作为磷吸附剂,铜功能化蛋壳膜生物炭(Cu@ESM)作为磷酸化催化剂。结果表明,该综合体系在弱碱性条件下(pH 8 ~ 9)具有良好的处理性能。在实验室规模的高浓度含磷合成废水(400 mg P/L)处理中,该系统在达到50%突破之前稳定了约940 min。中试(100 L/h)进一步用真实的猪废水进行验证,确认该系统在饱和前可连续处理废水约582 L,运行稳定高效。此外,通过优化钙磷摩尔比,可从浓缩洗脱液中回收高纯度的羟基磷灰石。本研究提出了一种可扩展的基于农业废弃物增值和高级废水处理一体化的技术,为磷的可持续管理和回收提供了一种有前途的解决方案。
{"title":"Dual-Functional Eggshell-Derived system for comprehensive phosphorus recovery from agricultural wastewater: From laboratory validation to Pilot-Scale implementation","authors":"Qiao Wu, Maoqing Fan, Xiaoyu Zheng, Ming Zeng, Saihong Ru, Chengyou Sun, Chao Huang","doi":"10.1016/j.biortech.2026.134192","DOIUrl":"https://doi.org/10.1016/j.biortech.2026.134192","url":null,"abstract":"The development of sustainable and economically feasible phosphorus recovery technology is of great significance to alleviate water eutrophication. This study proposed an innovative “one-waste, dual-use” strategy, involving the construction of a three-stage fixed-bed system integrating adsorption and Fenton-like oxidation. This system uses eggshell calcium-rich biochar (ESBC) as the phosphorus adsorbent and copper-functionalized eggshell membrane biochar (Cu@ESM) as the phosphorisation catalyst. The results showed that the integrated system has excellent treatment performance under weak alkaline conditions (pH 8–9). In the laboratory-scale treatment of high-concentration phosphorus-containing synthetic wastewater (400 mg P/L), the system was stable for approximately 940 min before reaching a 50% breakthrough. The pilot-scale test (100 L/h) was further validated with real pig wastewater, and it was confirmed that the system could continuously treat about 582 L of wastewater before saturation, and the operation was stable and efficient. Furthermore, by optimizing the molar ratio of calcium to phosphorus, high-purity hydroxyapatite was recovered from the concentrated eluent. This study presents a scalable technology based on the integration of agricultural waste valorization and advanced wastewater treatment, providing a promising solution for sustainable phosphorus management and recovery.","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"93 1","pages":""},"PeriodicalIF":11.4,"publicationDate":"2026-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146146356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Anodic potential and conductive carrier synergistically drive nitrite-free extracellular electron transfer-dependent anaerobic ammonium oxidation (Anammox) in mixed microbial communities 在混合微生物群落中,阳极电位和导电载体协同驱动无亚硝酸盐的细胞外电子转移依赖厌氧氨氧化(Anammox)
IF 11.4 1区 环境科学与生态学 Q1 AGRICULTURAL ENGINEERING Pub Date : 2026-02-09 DOI: 10.1016/j.biortech.2026.134180
Yang-Guang Xia, Jun-Hong Zhou, Xiao-Li Yang, Fei-Fan Shi, Ru Fan, Jia-Ying Xu
Anaerobic ammonium oxidation (Anammox) provides a low-carbon pathway for nitrogen removal, yet its reliance on nitrite (NO2-N) constrains large-scale application. Emerging evidence indicates that Anammox bacteria (AnAOB) can oxidize ammonium (NH4+-N) through extracellular electron transfer (EET) without NO2-N. However, the long-term stability of this process in mixed communities remains unresolved. Here, microbial electrolysis cells were operated for 260 days to investigate how anodic potential and conductive carrier regulate EET-dependent Anammox. A threshold potential of 0.4–0.6 V (vs. SHE) enabled NO2-N-free NH4+-N removal of 103.61 ± 9.22 mg N·L−1·d−1 (approximately 2.5-fold higher than highly enriched communities) via a hydroxylamine oxidoreductase-mediated pathway. The conductive carrier increased electron flux 4.9-fold, enhanced protein secretion, and stabilized biofilms. High potential combined with conductive carrier enriched electroactive AnAOB (Candidatus Kuenenia, Candidatus Brocadia) and induced a shift from NO2-N-dependent to EET-dependent metabolism. These findings demonstrate sustained long-term EET-dependent Anammox and inform scalable, carbon-free nitrogen removal.
厌氧氨氧化(Anammox)提供了一种低碳脱氮途径,但其对亚硝酸盐(NO2−-N)的依赖限制了其大规模应用。越来越多的证据表明,厌氧氨氧化菌(AnAOB)可以在没有NO2−-N的情况下通过胞外电子转移(EET)氧化铵(NH4+-N)。然而,这一进程在混合社区的长期稳定性仍未得到解决。在这里,微生物电解细胞运行260 天,以研究阳极电位和导电载体如何调节eet依赖性厌氧氨氧化。阈值电位为0.4-0.6 V(相对于SHE)使无NO2−-N的NH4+-N通过羟胺氧化还原酶介导的途径脱除103.61 ± 9.22 mg N·L−1·d−1(约为高富集群落的2.5倍)。导电载体使电子通量增加4.9倍,促进蛋白质分泌,稳定生物膜。高电位结合导电载体富集电活性AnAOB (Candidatus Kuenenia, Candidatus Brocadia),诱导代谢从NO2−- n依赖转变为eet依赖。这些发现证明了长期持续依赖eet的厌氧氨氧化,并为可扩展的无碳脱氮提供了信息。
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引用次数: 0
Mechanistic insights into phenolic compounds removal in constructed wetlands: efficiency comparison, synergistic interactions, and future optimization 人工湿地去除酚类化合物的机理:效率比较、协同作用和未来优化
IF 11.4 1区 环境科学与生态学 Q1 AGRICULTURAL ENGINEERING Pub Date : 2026-02-09 DOI: 10.1016/j.biortech.2026.134185
Zigeng Yu, Zhiyong Zhang, Zhikun Zou, Tianyu Shi, Yang Huo, Huachun Lan, Baoling Yuan, Ming-Lai Fu
Phenolic compounds (PCs), as persistent endocrine-disrupting chemicals, present a global environmental challenge. This review synthesizes the removal mechanisms of PCs in constructed wetlands (CWs), comparing the performance of different configurations. Results indicate that vertical flow CWs (VF-CWs) achieve superior removal of phenol (Ph) and bisphenol A (BPA) under aerobic conditions, primarily through the synergy of substrate adsorption and aerobic biodegradation, outperforming horizontal flow CWs (HF-CWs). Plant-microbe synergy is crucial, with vegetated systems enhancing the removal of BPA and nonylphenol (NP) via rhizosphere microbial enrichment and increased enzymatic activity. Hybrid CWs (Hy-CWs) with multi-stage units demonstrate higher removal efficiencies than single-stage systems, underscoring the advantages of integrated processes. Furthermore, the regulatory roles of key operational parameters, including substrate properties, temperature, and dissolved oxygen, are discussed. These insights provide a theoretical basis for optimizing CWs and outline future research priorities, including functional material development, multi-process technology coupling, and ecological safety assessment frameworks.
酚类化合物作为一种持久性内分泌干扰物质,对全球环境构成了挑战。本文综述了人工湿地中pc的去除机理,并对不同配置的性能进行了比较。结果表明,在好氧条件下,垂直流化化水(VF-CWs)主要通过底物吸附和好氧生物降解的协同作用,对苯酚(Ph)和双酚A (BPA)的去除效果优于水平流化化水(HF-CWs)。植物与微生物的协同作用是至关重要的,植被系统通过根际微生物的富集和酶活性的增加,增强了双酚a和壬基酚(NP)的去除。具有多级装置的混合化粪池(Hy-CWs)比单级系统具有更高的去除效率,强调了集成工艺的优势。此外,还讨论了关键操作参数的调节作用,包括衬底性质、温度和溶解氧。这些见解为优化化学武器提供了理论基础,并概述了未来的研究重点,包括功能材料开发、多工艺技术耦合和生态安全评估框架。
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引用次数: 0
Integration of real-time NH4+-N control and spatial microbial engineering achieves high removals of nitrogen and carbon in a sequence anoxic-oxic-anoxic (SAOA) system 实时NH4+-N控制与空间微生物工程相结合,实现了序列厌氧-厌氧-厌氧(SAOA)系统的高氮、高碳去除率
IF 11.4 1区 环境科学与生态学 Q1 AGRICULTURAL ENGINEERING Pub Date : 2026-02-09 DOI: 10.1016/j.biortech.2026.134189
Chaolong Gao, Qianwen Sui, Qihe Tang, Junya Zhang, Bing Yan, Dawei Yu, Fumin Zuo, Shuanglin Gui, Zhibo Liu, Xiaoshan Hu, Yuansong Wei
The imbalance among nitrite supply, nitrate accumulation and aeration demand poses significant challenges in single-stage partial nitrification-anammox (PN/A) systems for stably treating high-strength anaerobically digested swine wastewater (ADSW) at low C/N ratio. Here, we propose a novel process that integrates real-time NH4+-N control with a floc-granule partitioned biomass architecture in a sequence anoxic–oxic-anoxic (SAOA) system to dynamically modulate free ammonia (FA) concentration while preventing free nitrous acid (FNA) inhibition. By maintaining an NH4+-N endpoint of 50 mg/L, FA was stabilized at 5.2 mg N/L, and FNA was effectively suppressed. Thus, the SAOA system achieved 94.14% TN removal at a loading rate of 0.24 kg N/(m3·d) and 92.71% COD at low influent COD/TN ratio of 1.71, respectively. Metagenomic and enzymatic profiling revealed a distinct ecological stratification: floccular biomass was enriched with Candidatus Kuenenia, whereas granular microenvironments favored ammonia-oxidizing bacteria (AOB), accompanied by the upregulation of key nitrification and anammox genes. Kinetic analysis of COD and NH4+-N removals revealed a stage-specific metabolic transition from carbon-driven to autotrophic nitrogen-dominated removal. This study provides mechanistically robust and scalable control paradigm for advancing simultaneous, high-efficiency nitrogen and carbon removal from nitrogen-rich and carbon-limited wastewater.
单级部分硝化-厌氧氨氧化(PN/A)系统在低碳氮比条件下稳定处理高强度厌氧消化猪废水(ADSW)时,亚硝酸盐供应、硝酸盐积累和曝气需求之间的不平衡是一个重大挑战。在这里,我们提出了一种新的工艺,将实时NH4+-N控制与絮凝颗粒分割生物质结构结合在一个序列缺氧-缺氧-缺氧(SAOA)系统中,动态调节游离氨(FA)浓度,同时防止游离亚硝酸盐(FNA)抑制。通过维持NH4+-N终点50 mg/L, FA稳定在5.2 mg N/L, FNA得到有效抑制。结果表明,SAOA系统在进水COD/TN比为1.71时,进水COD/TN比为0.24 kg N/(m3·d), TN去除率为94.14%;进水COD/TN比为1.71时,COD去除率为92.71%。宏基因组和酶分析显示了明显的生态分层:絮状生物量富含Kuenenia,而颗粒微环境则有利于氨氧化细菌(AOB),并伴有关键硝化和厌氧基因的上调。COD和NH4+-N去除的动力学分析揭示了从碳驱动到自养氮主导去除的特定阶段代谢转变。该研究为推进富氮和限碳废水的同时高效脱氮和脱碳提供了机制稳健和可扩展的控制范式。
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Bioresource Technology
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