Pub Date : 2024-09-30DOI: 10.1016/j.biortech.2024.131559
Zhongqi He, Xu Zhou, Luyao Qu, Wenbiao Jin, Xuan Li, Huan Liu, Qilin Wang
This study integrates electrochemical pretreatment with microalgae (Scenedesmus obliquus) treatment to enhance nitrogen and phosphorus removal and resource recovery from swine wastewater. By optimizing electrochemical and microalgae treatment conditions, the dilution factor and the hydraulic retention time for microalgae treatment were reduced to 5 times and 7 days, respectively. Under the optimized operational conditions, removal efficiencies of total nitrogen and ammonia could reach over 89 %and 96 %,respectively, and the removal efficiency of total phosphorus was over 99 %. The study also found that aluminum was more suitable than iron for anode as it produced fewer residues. Additionally, the electrochemical pretreatment reduced Cu2+ and Zn2+ concentrations, mitigating negative impacts on microalgal growth. The microalgae biomass harvested from developed processes was rich in saturated fatty acids, which was desirable for biodiesel production. This approach addresses the challenges of nutrient removal for swine wastewater treatment with high quality biomass recovery.
{"title":"Integrating electrochemical pretreatment with microalgae treatment for nitrogen and phosphorus removal and resource recovery from swine wastewater.","authors":"Zhongqi He, Xu Zhou, Luyao Qu, Wenbiao Jin, Xuan Li, Huan Liu, Qilin Wang","doi":"10.1016/j.biortech.2024.131559","DOIUrl":"https://doi.org/10.1016/j.biortech.2024.131559","url":null,"abstract":"<p><p>This study integrates electrochemical pretreatment with microalgae (Scenedesmus obliquus) treatment to enhance nitrogen and phosphorus removal and resource recovery from swine wastewater. By optimizing electrochemical and microalgae treatment conditions, the dilution factor and the hydraulic retention time for microalgae treatment were reduced to 5 times and 7 days, respectively. Under the optimized operational conditions, removal efficiencies of total nitrogen and ammonia could reach over 89 %and 96 %,respectively, and the removal efficiency of total phosphorus was over 99 %. The study also found that aluminum was more suitable than iron for anode as it produced fewer residues. Additionally, the electrochemical pretreatment reduced Cu<sup>2+</sup> and Zn2<sup>+</sup> concentrations, mitigating negative impacts on microalgal growth. The microalgae biomass harvested from developed processes was rich in saturated fatty acids, which was desirable for biodiesel production. This approach addresses the challenges of nutrient removal for swine wastewater treatment with high quality biomass recovery.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":null,"pages":null},"PeriodicalIF":9.7,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142363681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-30DOI: 10.1016/j.biortech.2024.131560
Minsu Song, Jihye Park, Joonyeob Lee, Hyokwan Bae
This study examined integrating high-rate contact stabilization (HRCS) and chemically enhanced primary treatment (CEPT) for wastewater to improve the carbon recovery rate (CRR). Enhancing chemical oxygen demand (COD) removal efficiency was hypothesized to improve the CRR. The evaluation covered serial HRCS-CEPT, serial CEPT-HRCS, and single-stage carbon recovery (Single-CR). The COD removal efficiencies for individual HRCS and CEPT were 50.3 % and 56.2 %, respectively. The serial CEPT-HRCS system failed in the HRCS process due to poor settling, resulting in microbial washout. However, the serial HRCS-CEPT system achieved the highest COD removal efficiency (84.5 %). The Single-CR system exhibited the highest CRR of 0.780 ± 0.083 g-CODCH4/g-CODinf, identifying it as the most promising process for energy-positive wastewater treatment. The selective pressure in the high-rate system resulted in a simplified and specialized bacterial community, mainly comprising microorganisms with high polyhydroxyalkanoate storage capacity, such as Lactococcus sp., Enterobacter sp., and Acinetobacter sp.
{"title":"Combined high-rate contact stabilization and chemically enhanced primary treatment for enhanced recovery of organic matter and biogas from sewage.","authors":"Minsu Song, Jihye Park, Joonyeob Lee, Hyokwan Bae","doi":"10.1016/j.biortech.2024.131560","DOIUrl":"https://doi.org/10.1016/j.biortech.2024.131560","url":null,"abstract":"<p><p>This study examined integrating high-rate contact stabilization (HRCS) and chemically enhanced primary treatment (CEPT) for wastewater to improve the carbon recovery rate (CRR). Enhancing chemical oxygen demand (COD) removal efficiency was hypothesized to improve the CRR. The evaluation covered serial HRCS-CEPT, serial CEPT-HRCS, and single-stage carbon recovery (Single-CR). The COD removal efficiencies for individual HRCS and CEPT were 50.3 % and 56.2 %, respectively. The serial CEPT-HRCS system failed in the HRCS process due to poor settling, resulting in microbial washout. However, the serial HRCS-CEPT system achieved the highest COD removal efficiency (84.5 %). The Single-CR system exhibited the highest CRR of 0.780 ± 0.083 g-COD<sub>CH4</sub>/g-COD<sub>inf</sub>, identifying it as the most promising process for energy-positive wastewater treatment. The selective pressure in the high-rate system resulted in a simplified and specialized bacterial community, mainly comprising microorganisms with high polyhydroxyalkanoate storage capacity, such as Lactococcus sp., Enterobacter sp., and Acinetobacter sp.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":null,"pages":null},"PeriodicalIF":9.7,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142363677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Polyethylene terephthalate (PET) biodegradation is hindered by the intermediates bis (2-hydroxyethyl) terephthalate (BHET) and mono (2-hydroxyethyl) terephthalate (MHET). BMHETase, a thermophilic hydrolase identified from the UniParc database, exhibits degradation activity towards both BHET and MHET. BMHETase showed higher activity on BHET than LCCICCG and FASTPETase at temperatures ranging from 50 to 70℃. To enhance its activity in degrading MHET, BMHETase was engineered to mimic Ideonella sakaiensis MHETase. The resulting 6-point mutant's activities on MHET and BHET were 8 and 2 times those of the WT, with both optimal temperatures increased by 5℃. This enhancement may be attributed to the BMHETase6M's intensified binding ability with MHET and enlarged binding pocket. When combined with LCCICCG, BMHETase6M achieved complete degradation of MHET in PET films to terephthalic acid, indicating broad application potential. These findings suggest that BMHETase6M holds promise as a candidate for enhancing PET biodegradation efficiency and plastic waste management.
{"title":"Engineering dual-functional and thermophilic BMHETase for efficient degradation of polyethylene terephthalate.","authors":"Ruiju Miao, Guoshun Xu, Yekun Ding, Zundan Ding, Jaie Woodard, Tao Tu, Huiying Luo, Ningfeng Wu, Bin Yao, Feifei Guan, Jian Tian","doi":"10.1016/j.biortech.2024.131556","DOIUrl":"https://doi.org/10.1016/j.biortech.2024.131556","url":null,"abstract":"<p><p>Polyethylene terephthalate (PET) biodegradation is hindered by the intermediates bis (2-hydroxyethyl) terephthalate (BHET) and mono (2-hydroxyethyl) terephthalate (MHET). BMHETase, a thermophilic hydrolase identified from the UniParc database, exhibits degradation activity towards both BHET and MHET. BMHETase showed higher activity on BHET than LCCICCG and FASTPETase at temperatures ranging from 50 to 70℃. To enhance its activity in degrading MHET, BMHETase was engineered to mimic Ideonella sakaiensis MHETase. The resulting 6-point mutant's activities on MHET and BHET were 8 and 2 times those of the WT, with both optimal temperatures increased by 5℃. This enhancement may be attributed to the BMHETase<sup>6M</sup>'s intensified binding ability with MHET and enlarged binding pocket. When combined with LCCICCG, BMHETase<sup>6M</sup> achieved complete degradation of MHET in PET films to terephthalic acid, indicating broad application potential. These findings suggest that BMHETase<sup>6M</sup> holds promise as a candidate for enhancing PET biodegradation efficiency and plastic waste management.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":null,"pages":null},"PeriodicalIF":9.7,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142363678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The nitrogen removal of anaerobically digested swine wastewater (ADSW) through partial nitritation and anammox is hindered by the challenge of balancing aeration between ammonia oxidizing bacteria (AOB) and anammox bacteria (AnAOB). This study focused on optimizing aeration through a real-time control strategy in an integrated fixed-film activated sludge reactor for treating ADSW. The system implemented a dual aeration mode that included both low dissolved oxygen (DO) (< 0.4 mg/L) and short-term high DO (0.6-1.2 mg/L), with pH, oxidation-reduction potential, and NH4+-N electrode values as real-time control parameters. NH4+-N removal rate increased from 3.37 to 12.82 mgN/(gVSS·h), and total nitrogen (TN) removal rate enhanced from 0.14 to 0.25 kgN/(m3·d). Increasing DO stimulated AOB activity by 31 % and provided sufficient NO2--N for AnAOB. The r-strategist AnAOB (Candidatus Kuenenia) proliferated well in the biofilm (0.25 % in flocs vs. 1.86 % in biofilm). The enrichment of denitrifiers improved organic matter and TN removal.
{"title":"Enhancing nitrogen removal from digested swine wastewater by anammox with aeration optimization coupling real-time control strategy.","authors":"Chaolong Gao, Qianwen Sui, Fumin Zuo, Wenhui Yue, Yuansong Wei","doi":"10.1016/j.biortech.2024.131554","DOIUrl":"https://doi.org/10.1016/j.biortech.2024.131554","url":null,"abstract":"<p><p>The nitrogen removal of anaerobically digested swine wastewater (ADSW) through partial nitritation and anammox is hindered by the challenge of balancing aeration between ammonia oxidizing bacteria (AOB) and anammox bacteria (AnAOB). This study focused on optimizing aeration through a real-time control strategy in an integrated fixed-film activated sludge reactor for treating ADSW. The system implemented a dual aeration mode that included both low dissolved oxygen (DO) (< 0.4 mg/L) and short-term high DO (0.6-1.2 mg/L), with pH, oxidation-reduction potential, and NH<sub>4</sub><sup>+</sup>-N electrode values as real-time control parameters. NH<sub>4</sub><sup>+</sup>-N removal rate increased from 3.37 to 12.82 mgN/(gVSS·h), and total nitrogen (TN) removal rate enhanced from 0.14 to 0.25 kgN/(m<sup>3</sup>·d). Increasing DO stimulated AOB activity by 31 % and provided sufficient NO<sub>2</sub><sup>-</sup>-N for AnAOB. The r-strategist AnAOB (Candidatus Kuenenia) proliferated well in the biofilm (0.25 % in flocs vs. 1.86 % in biofilm). The enrichment of denitrifiers improved organic matter and TN removal.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":null,"pages":null},"PeriodicalIF":9.7,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142363679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This review explores the critical role of machine learning (ML) in enhancing microalgae bioprocesses for sustainable biofuel production. It addresses both technical and economic challenges in commercializing microalgal biofuels and examines how ML can optimize various stages, including identification, classification, cultivation, harvesting, drying, and conversion to biofuels. This review also highlights the integration of ML with technologies such as the Internet of Things (IoT) for real-time monitoring and management of bioprocesses. It discusses the adaptability and flexibility of ML in the context of microalgae biotechnology, focusing on diverse algorithms such as Artificial Neural Networks, Support Vector Machines, Decision Trees, and Random Forests, while emphasizing the importance of data collection and preparation. Additionally, current ML applications in microalgae biofuel production are reviewed, including strain selection, growth optimization, system monitoring, and lipid extraction.
本综述探讨了机器学习(ML)在提高微藻生物工艺以实现可持续生物燃料生产方面的关键作用。它探讨了微藻生物燃料商业化过程中的技术和经济挑战,并研究了机器学习如何优化各个阶段,包括识别、分类、栽培、收获、干燥和转化为生物燃料。本综述还重点介绍了如何将 ML 与物联网 (IoT) 等技术相结合,对生物过程进行实时监控和管理。它讨论了微藻生物技术背景下 ML 的适应性和灵活性,重点介绍了人工神经网络、支持向量机、决策树和随机森林等各种算法,同时强调了数据收集和准备的重要性。此外,还回顾了当前微藻生物燃料生产中的 ML 应用,包括菌株选择、生长优化、系统监控和脂质提取。
{"title":"Machine learning in microalgae biotechnology for sustainable biofuel production: Advancements, applications, and prospects.","authors":"Chao-Tung Yang, Endah Kristiani, Yoong Kit Leong, Jo-Shu Chang","doi":"10.1016/j.biortech.2024.131549","DOIUrl":"10.1016/j.biortech.2024.131549","url":null,"abstract":"<p><p>This review explores the critical role of machine learning (ML) in enhancing microalgae bioprocesses for sustainable biofuel production. It addresses both technical and economic challenges in commercializing microalgal biofuels and examines how ML can optimize various stages, including identification, classification, cultivation, harvesting, drying, and conversion to biofuels. This review also highlights the integration of ML with technologies such as the Internet of Things (IoT) for real-time monitoring and management of bioprocesses. It discusses the adaptability and flexibility of ML in the context of microalgae biotechnology, focusing on diverse algorithms such as Artificial Neural Networks, Support Vector Machines, Decision Trees, and Random Forests, while emphasizing the importance of data collection and preparation. Additionally, current ML applications in microalgae biofuel production are reviewed, including strain selection, growth optimization, system monitoring, and lipid extraction.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":null,"pages":null},"PeriodicalIF":9.7,"publicationDate":"2024-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142338132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-28DOI: 10.1016/j.biortech.2024.131523
Zheng-Kai An, Han-Chao Yu, Keug-Tae Kim, Yongtae Ahn, Qing Feng, Young-Chae Song
The performance and stability of a bioelectrochemical anaerobic digester (BeAD), continuously augmented with electroactive microorganisms (EAMs), were investigated. The BeAD showcased superior performance, sustaining the high COD removal efficiency and methane production rate of 76.5 % and 0.67 L/(L.d), respectively, in a stable state. Prominently, it exhibited remarkable resilience under hydraulic and organic shock loads, adeptly recuperating from disturbances up to 1000 % of its stable condition. This resilience of up to 300 % shock load was driven by increased levels of electron transport components such as quinones and riboflavins, which act as electron shuttles. However, after extreme shock exposures from 500 % to 1000 %, despite the spike in inhibitory by-products such as humic acids and ammonia, the upregulation of the mtr complex was pivotal in recovering and sustaining methane production. These insights emphasize the BeAD's capability to bolster both performance and stability, thereby providing a potent strategy for practical application of bioelectrochemical systems.
{"title":"Continuous augmentation of anaerobic digestion with electroactive microorganisms: Performance and stability.","authors":"Zheng-Kai An, Han-Chao Yu, Keug-Tae Kim, Yongtae Ahn, Qing Feng, Young-Chae Song","doi":"10.1016/j.biortech.2024.131523","DOIUrl":"10.1016/j.biortech.2024.131523","url":null,"abstract":"<p><p>The performance and stability of a bioelectrochemical anaerobic digester (BeAD), continuously augmented with electroactive microorganisms (EAMs), were investigated. The BeAD showcased superior performance, sustaining the high COD removal efficiency and methane production rate of 76.5 % and 0.67 L/(L.d), respectively, in a stable state. Prominently, it exhibited remarkable resilience under hydraulic and organic shock loads, adeptly recuperating from disturbances up to 1000 % of its stable condition. This resilience of up to 300 % shock load was driven by increased levels of electron transport components such as quinones and riboflavins, which act as electron shuttles. However, after extreme shock exposures from 500 % to 1000 %, despite the spike in inhibitory by-products such as humic acids and ammonia, the upregulation of the mtr complex was pivotal in recovering and sustaining methane production. These insights emphasize the BeAD's capability to bolster both performance and stability, thereby providing a potent strategy for practical application of bioelectrochemical systems.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":null,"pages":null},"PeriodicalIF":9.7,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142338118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-27DOI: 10.1016/j.biortech.2024.131548
Mengyao Liu, Shenbao Qu, Hongdi Mou, Man Wei, Xia Hu, Aijiang Yang
A novel molecularly imprinted biomass carbon (MIP@BC) catalyst functionalized with the virtual template of phthalates was designed as the cathode material which possesses excellent 2-electron oxygen reduction ability and H2O2 production capacity, which is suitable for targeted degradation of phthalates in the electro-Fenton system. Following molecularly imprinted modification, the adsorption capacity of MIP@BC for Dimethyl phthalate (DMP) increased by 40 %, reached 9.26 mg/g. Compared with non-imprinted biomass carbon (NIP@BC), the MIP@BC-mediated electro-Fenton process enhanced the degradation rate of DMP by 72 %. Additionally, the degradation rate of DMP rises by 51 % and 104 % respectively on the basis of river water and domestic sewage. The reactive oxygen species that induced DMP degradation were OH and O2- and targeted adsorption and catalysis exert a synergistic effect. This study provides a new insight into targeted degradation for high-toxicity of emerging contaminants from complex aqueous environment.
{"title":"Selective phthalate removal by molecularly imprinted biomass carbon modified electro-Fenton cathode.","authors":"Mengyao Liu, Shenbao Qu, Hongdi Mou, Man Wei, Xia Hu, Aijiang Yang","doi":"10.1016/j.biortech.2024.131548","DOIUrl":"https://doi.org/10.1016/j.biortech.2024.131548","url":null,"abstract":"<p><p>A novel molecularly imprinted biomass carbon (MIP@BC) catalyst functionalized with the virtual template of phthalates was designed as the cathode material which possesses excellent 2-electron oxygen reduction ability and H<sub>2</sub>O<sub>2</sub> production capacity, which is suitable for targeted degradation of phthalates in the electro-Fenton system. Following molecularly imprinted modification, the adsorption capacity of MIP@BC for Dimethyl phthalate (DMP) increased by 40 %, reached 9.26 mg/g. Compared with non-imprinted biomass carbon (NIP@BC), the MIP@BC-mediated electro-Fenton process enhanced the degradation rate of DMP by 72 %. Additionally, the degradation rate of DMP rises by 51 % and 104 % respectively on the basis of river water and domestic sewage. The reactive oxygen species that induced DMP degradation were OH and O<sub>2</sub><sup>-</sup> and targeted adsorption and catalysis exert a synergistic effect. This study provides a new insight into targeted degradation for high-toxicity of emerging contaminants from complex aqueous environment.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":null,"pages":null},"PeriodicalIF":9.7,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142338134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-27DOI: 10.1016/j.biortech.2024.131546
This work developed a method using bamboo shoot shells as raw material to produce Fe-modified ACs combining self-activation and chemical modification. Adding small amounts (0.5–5 %) of K2FeO4 accelerated the pyrolysis process and CO2 release, and reduced the activation energy and temperature of self-activation reaction. This increased the reaction rate and activation efficiency, ultimately significantly improving the pore structure of ACs. The addition of 3 % K2FeO4 resulted in a substantial increase in specific surface area and pore volume of AC, from 1340 m2/g and 0.72 cm3/g to 2184 m2/g and 1.34 cm3/g, respectively. Additionally, the introduction of K2FeO4 also enabled iron doping on the surface of the ACs. The improvement of pore structure and iron doping further enhanced the adsorption performance of ACs. The adsorption capacities of ACs for arsenate, ibuprofen, and tetracycline were up to 1.64, 1.50, and 2.38 times higher, respectively, than those of ACs prepared through conventional self-activation.
这项研究开发了一种以竹笋壳为原料,结合自活化和化学改性生产铁改性 AC 的方法。添加少量(0.5-5 %)K2FeO4可加速热解过程和二氧化碳的释放,降低自活化反应的活化能和温度。这提高了反应速率和活化效率,最终显著改善了 AC 的孔结构。加入 3 % K2FeO4 后,AC 的比表面积和孔隙体积大幅增加,分别从 1340 m2/g 和 0.72 cm3/g 增加到 2184 m2/g 和 1.34 cm3/g。此外,K2FeO4 的引入还使 AC 表面掺杂了铁。孔隙结构的改善和铁的掺杂进一步提高了 AC 的吸附性能。ACs 对砷酸盐、布洛芬和四环素的吸附容量分别是传统自激活制备的 ACs 的 1.64 倍、1.50 倍和 2.38 倍。
{"title":"Enhancement effect of potassium ferrate on self-activation: Significant improvement in pore structure and adsorption performance of activated carbon","authors":"","doi":"10.1016/j.biortech.2024.131546","DOIUrl":"10.1016/j.biortech.2024.131546","url":null,"abstract":"<div><div>This work developed a method using bamboo shoot shells as raw material to produce Fe-modified ACs combining self-activation and chemical modification. Adding small amounts (0.5–5 %) of K<sub>2</sub>FeO<sub>4</sub> accelerated the pyrolysis process and CO<sub>2</sub> release, and reduced the activation energy and temperature of self-activation reaction. This increased the reaction rate and activation efficiency, ultimately significantly improving the pore structure of ACs. The addition of 3 % K<sub>2</sub>FeO<sub>4</sub> resulted in a substantial increase in specific surface area and pore volume of AC, from 1340 m<sup>2</sup>/g and 0.72 cm<sup>3</sup>/g to 2184 m<sup>2</sup>/g and 1.34 cm<sup>3</sup>/g, respectively. Additionally, the introduction of K<sub>2</sub>FeO<sub>4</sub> also enabled iron doping on the surface of the ACs. The improvement of pore structure and iron doping further enhanced the adsorption performance of ACs. The adsorption capacities of ACs for arsenate, ibuprofen, and tetracycline were up to 1.64, 1.50, and 2.38 times higher, respectively, than those of ACs prepared through conventional self-activation.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":null,"pages":null},"PeriodicalIF":9.7,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142327562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-27DOI: 10.1016/j.biortech.2024.131547
Yifan Niu, Siyao Wang, Peng Gao, Xin Ren, Fangfang Li, Zhanpeng Liu, Lin Wang, Hongbo Peng, Shaohua Ju
This study explores the physicochemical attributes of dissolved organic matter from rice straw biochar (BDOM) at varying pyrolysis temperatures and photo-irradiation conditions, focusing on the binding mechanisms of phenanthrene (PHE) and 9-phenanthrol (PTR) using multiple spectroscopic techniques and fluorescence quenching. Following 20 h of photo-irradiation, only 11.3 % of BDOM underwent mineralization, forming new CH3/CH2/CH aliphatics structures. BDOM from biochar produced by pyrolysis at 400°C exhibited a stronger binding affinity with PHE and PTR, achieving 44 % and 52 % maximum binding, respectively. Static and dynamic quenching governed PHE and PTR binding, which was influenced by temperature. Photo-irradiated BDOM showed enhanced binding with PHE, attributed to increased aliphatic content. Hydrogen bond and π-π electron-donor–acceptor (EDA) interactions dominated PTR binding, while π-π interactions and hydrophobic interactions controlled PHE. This study provides valuable insights into BDOM photochemical behaviors and their impact on the environmental fate of polycyclic aromatic hydrocarbons (PAHs) after BDOM photo-irradiation.
{"title":"Photo-transformation of biochar-derived dissolved organic matter and its binding with phenanthrene/9-phenanthrol: The role of functional group and pyrolysis temperature","authors":"Yifan Niu, Siyao Wang, Peng Gao, Xin Ren, Fangfang Li, Zhanpeng Liu, Lin Wang, Hongbo Peng, Shaohua Ju","doi":"10.1016/j.biortech.2024.131547","DOIUrl":"https://doi.org/10.1016/j.biortech.2024.131547","url":null,"abstract":"This study explores the physicochemical attributes of dissolved organic matter from rice straw biochar (BDOM) at varying pyrolysis temperatures and photo-irradiation conditions, focusing on the binding mechanisms of phenanthrene (PHE) and 9-phenanthrol (PTR) using multiple spectroscopic techniques and fluorescence quenching. Following 20 h of photo-irradiation, only 11.3 % of BDOM underwent mineralization, forming new CH<ce:inf loc=\"post\">3</ce:inf>/CH<ce:inf loc=\"post\">2</ce:inf>/CH aliphatics structures. BDOM from biochar produced by pyrolysis at 400°C exhibited a stronger binding affinity with PHE and PTR, achieving 44 % and 52 % maximum binding, respectively. Static and dynamic quenching governed PHE and PTR binding, which was influenced by temperature. Photo-irradiated BDOM showed enhanced binding with PHE, attributed to increased aliphatic content. Hydrogen bond and π-π electron-donor–acceptor (EDA) interactions dominated PTR binding, while π-π interactions and hydrophobic interactions controlled PHE. This study provides valuable insights into BDOM photochemical behaviors and their impact on the environmental fate of polycyclic aromatic hydrocarbons (PAHs) after BDOM photo-irradiation.","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":null,"pages":null},"PeriodicalIF":11.4,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142329786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-27DOI: 10.1016/j.biortech.2024.131492
Peng Zhang, Xin-An Mao, Jin-Song Gong, Xiao-Li Kong, Chang Su, Xiao-Mei Zhang, Zhi-Ming Rao, Zheng-Hong Xu, Jin-Song Shi
Although Bacillus subtilis shows promise as a potential microbial cell factory for phospholipase D (PLD) expression, its production capacity remains insufficient. In this study, a secretory expression system, by co-optimization the promoter and signal peptides and employing a fed-batch fermentation strategy, was constructed to enhance expression of PLD from separate sources. The highest PLD production of 4056.9 U/mL was observed using this system, with a PLD production efficiency of 52.0 U/mL/h. Finally, a phosphatidic acid (PA) biosynthesis system was established using the constructed PLD as a catalyst, which achieved a PA yield of 219.1 g/L. This is the highest PLD production and PA yield reported globally to date. The protocol has significant potential for application for industrial PLD production as well as enzymatic phospholipids modification and also provides a valuable reference for overexpressing proteins in B. subtilis.
{"title":"High-level extracellular expression of phospholipase D by combinatorial fine-tuning strategy in Bacillus subtilis for efficient biosynthesis of phosphatidic acid.","authors":"Peng Zhang, Xin-An Mao, Jin-Song Gong, Xiao-Li Kong, Chang Su, Xiao-Mei Zhang, Zhi-Ming Rao, Zheng-Hong Xu, Jin-Song Shi","doi":"10.1016/j.biortech.2024.131492","DOIUrl":"https://doi.org/10.1016/j.biortech.2024.131492","url":null,"abstract":"<p><p>Although Bacillus subtilis shows promise as a potential microbial cell factory for phospholipase D (PLD) expression, its production capacity remains insufficient. In this study, a secretory expression system, by co-optimization the promoter and signal peptides and employing a fed-batch fermentation strategy, was constructed to enhance expression of PLD from separate sources. The highest PLD production of 4056.9 U/mL was observed using this system, with a PLD production efficiency of 52.0 U/mL/h. Finally, a phosphatidic acid (PA) biosynthesis system was established using the constructed PLD as a catalyst, which achieved a PA yield of 219.1 g/L. This is the highest PLD production and PA yield reported globally to date. The protocol has significant potential for application for industrial PLD production as well as enzymatic phospholipids modification and also provides a valuable reference for overexpressing proteins in B. subtilis.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":null,"pages":null},"PeriodicalIF":9.7,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142338130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}