Bioresource Technology最新文献_第3页

An integrated ultrasonic and cellulase pretreatment strategy: simultaneously enhancing medium-chain fatty acid production and reducing antibiotic resistance genes in anaerobic digestion of animal manure 超声与纤维素酶一体化预处理策略：在动物粪便厌氧消化过程中，同时提高中链脂肪酸产量，减少抗生素耐药基因

IF 9 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology

Pub Date : 2025-12-09 DOI: 10.1016/j.biortech.2025.133780

Bingni Wei , Li Zhang , Haoxiang Zhang , Bojun Gui , Qiuqing Deng , Xuan Chang , Peng Yu , Xianwen Dong , Bing Zou , Xiaoli Zhang , Yongju Zhao , Zhongquan Zhao , Juncai Chen

Rapid expansion of animal husbandry poses significant environmental challenges, including waste management and pollution. While medium-chain fatty acid (MCFA) production from livestock manure via chain elongation (CE) process in anaerobic digestion (AD) offers a promising and economically valuable waste management strategy, the associated risks of antibiotic resistance genes (ARGs) propagation through AD products are often overlooked. Additionally, the impact of substrate pretreatments on MCFA production and ARGs abundances in livestock manure during CE process remains unclear. This study assessed synergistic effects of cellulase and ultrasonic pretreatments on MCFA yield, microbial community, and ARGs during CE process of co-digesting lignocellulosic goat manure and corn straw. The results demonstrated that cellulase pretreatment increased caproate yield by 46.72 % (from 6.93 to 10.17 g of chemical oxygen demand (COD)/L). Combined cellulase and ultrasonic pretreatments decreased ARGs relative abundance by 25.28 %. The underlying mechanism was further explored through metagenome, revealing the roles of microbial community shifts and gene regulation in the beneficial outcomes. Specifically, we found that cellulase pretreatment enriched Clostridia and modulated genes related to reverse β-oxidation and ethanol oxidation, contributing to enhanced MCFA production. The reduction in ARGs abundance, particularly with combined pretreatments, was linked to a decrease in Massilibacterium abundance. Additionally, co-occurrence analysis indicated Actinomycetota, Bacillota, Bacteroidota, and Pseudomonadota as primary ARG hosts. This study demonstrates the viability of lignocellulosic goat manure for MCFA production via CE. Pretreatments effectively boost MCFA yield and mitigate ARGs, providing a basis for comprehensive biorefinery systems integrating resource valorization and ARGs control in lignocellulosic animal waste management.

畜牧业的迅速扩张带来了重大的环境挑战，包括废物管理和污染。虽然通过厌氧消化（AD）中的链延伸（CE）过程从畜禽粪便中生产中链脂肪酸（MCFA）提供了一种有前景且具有经济价值的废物管理策略，但通过AD产品传播抗生素抗性基因（ARGs）的相关风险往往被忽视。此外，基质预处理对CE过程中畜禽粪便中MCFA产量和ARGs丰度的影响尚不清楚。本研究评估了纤维素酶和超声波预处理对木质纤维素羊粪和玉米秸秆共消化过程中MCFA产量、微生物群落和ARGs的协同效应。结果表明，纤维素酶预处理使己酸产率提高46.72%（化学需氧量(COD)/L由6.93 g提高到10.17 g）。纤维素酶和超声波联合预处理可使ARGs相对丰度降低25.28%。通过宏基因组进一步探索其潜在机制，揭示微生物群落转移和基因调控在有益结果中的作用。具体来说，我们发现纤维素酶预处理富集了梭状芽孢杆菌并调节了与反向β-氧化和乙醇氧化相关的基因，从而促进了MCFA的产生。ARGs丰度的降低，特别是联合预处理，与马氏杆菌丰度的降低有关。此外，共发生分析表明放线菌门、杆菌门、拟杆菌门和假单胞菌门是ARG的主要宿主。本研究证明了木质纤维素羊粪通过CE生产MCFA的可行性。预处理有效地提高了MCFA产量，减少了ARGs，为木质纤维素动物粪便管理中整合资源价值和ARGs控制的综合生物炼制系统提供了基础。

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引用次数: 0

Unlocking dual-pathway peroxydisulfate activation mechanism in single atom Fe-based catalytic membranes for wastewater purification 单原子铁基催化膜废水净化中过氧化二硫酸盐双途径活化机理的研究

IF 11.4 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology

Pub Date : 2025-12-08 DOI: 10.1016/j.biortech.2025.133785

Ting Zhou, Fupeng Wang, Xuechuan Li, Wenyi Dong, Mengke Qiang, Weigang Liu, Hongjie Wang

Carbon-based catalytic membrane processes (CMPs) have emerged as promising technologies for wastewater decontamination, but challenges such as limited catalytic activity remain. This study developed a high-efficiency peroxydisulfate-based CMP (SAFe@GF/PDS) for aniline (AN) degradation via nonradical pathways, incorporating single-atom Fe sites with pyrrolic-N2O2 coordination geometry. Under a single-pass mode at a membrane flux of 3906.3 LMH, the system achieved > 90 % AN removal and maintained > 80 % efficiency in natural waters, demonstrating robust environmental adaptability. Mechanistic investigations elucidated that the well-designed mesoporous structure enabled optimal exposure of active sites, where monoatomic Fe sites and CC moieties predominantly generated metastable complexes PDS* (50.3 % contribution) and 1O2 (47.8 % contribution), respectively. This dual-channel activation mechanism, synergistically enhanced by electron-transfer-promoting O–CO groups, facilitated efficient AN degradation through four complementary reaction pathways while simultaneously minimizing toxic byproduct formation. By adjusting Fe, N, and KOH doping ratios, a quantitative structure–activity relationship for high-performance BC-SACs was established. Precise control of Fe doping amount was essential to maximize FeNx density while avoiding clusters formation. Modulation of surface functional groups, namely increasing electron-donating COC groups and synergistic O-species (Fe–O, OC–O) while suppressing electron-withdrawing amide groups was benifical. Fabrication of hierarchical porous structures with high specific surface area and large pore volume was vital to optimize mass transport. This work presented a new class of high-efficiency catalytic membranes and offered theoretical insights for the rational design of functional materials for sustainable wastewater treatment.

碳基催化膜工艺（CMPs）已成为污水净化的一项有前景的技术，但其催化活性有限等挑战仍然存在。本研究开发了一种高效的过氧二硫酸盐基CMP (SAFe@GF/PDS)，通过非自由基途径降解苯胺（AN），将单原子铁位与吡啶- n2o2配位结合在一起。在膜通量为3906.3 LMH的单次通过模式下，系统在自然水体中实现了 >； 90 %的AN去除率，并保持了 >； 80 %的效率，表现出强大的环境适应性。机制研究表明，设计良好的介孔结构使活性位点的暴露达到最佳，其中单原子Fe位点和CC部分主要产生亚稳配合物PDS*（贡献50.3% %）和1O2（贡献47.8 %）。这种双通道激活机制与促进电子转移的O-CO基团协同增强，通过四种互补的反应途径促进了AN的有效降解，同时最大限度地减少了有毒副产物的形成。通过调整Fe、N和KOH的掺杂比例，建立了高性能BC-SACs的定量构效关系。精确控制Fe掺杂量对于最大限度地提高FeNx密度和避免团簇形成至关重要。调节表面官能团，即增加给电子的COC基团和协同o -物种（Fe-O, OC-O），同时抑制吸电子的酰胺基团是有益的。制备具有高比表面积和大孔体积的分层多孔结构对于优化质量传输至关重要。本研究提出了一类新型高效催化膜，为可持续废水处理功能材料的合理设计提供了理论依据。

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引用次数: 0

Advanced nitrogen removal in anaerobic/aerobic/anoxic system under seasonal cooling: enhancing anammox performance in switchable unit 季节性冷却条件下厌氧/好氧/缺氧系统的深度脱氮：提高开关装置厌氧氨氧化性能

IF 9 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology

Pub Date : 2025-12-08 DOI: 10.1016/j.biortech.2025.133784

You Wu , Hanbin Wang , Xialian Shi , Qiong Zhang , Hongxun Hou , Yongzhen Peng

The robustness of anaerobic/aerobic/anoxic (AOA) process under low temperature remains insufficiently understood. This study investigated a continuous-flow AOA system while temperature decreased from 27.8 to 15.9 °C. Treating municipal wastewater (total nitrogen (TN) ≈ 75 mg/L; C/N ≈ 3.5), the system achieved a nitrogen removal efficiency (NRE) of 94 % and effluent TN of 4.2 mg/L at 24.7°C. When temperature fell below ∼22 °C, switching Unit 4 from anoxic to aerobic extended the aerobic retention time and sustained an NRE of 87 %, with Unit 4 contributing 13.7 mg/L of nitrogen removal. This adjustment increased biofilm biomass and improved transcription of key anammox genes despite reduced anammox abundance. Kinetic assays showed nitrification supplied nitrite faster than endogenous denitrification. Microbial analysis revealed persistence of oxygen-tolerant Ca. Brocadia (OTU2598 > 97 %). Improved nitrite availability and elevated gene transcription jointly supported stable performance, demonstrating the feasibility of dual-pathway anammox for mainstream treatment in temperate regions.

低温条件下厌氧/好氧/缺氧（AOA）工艺的稳健性尚不清楚。本文研究了温度从27.8℃降至15.9℃ ℃时的连续流动AOA系统。处理城市污水(总氮(TN)≈75 mg/L；C/N≈3.5)时，系统在24.7℃下的脱氮效率（NRE）为94 %，出水TN为4.2 mg/L。当温度降至~ 22 °C以下时，将4号机组从缺氧切换为好氧延长了好氧保留时间，并保持了87 %的NRE，其中4号机组的氮去除率为13.7 mg/L。这种调整增加了生物膜生物量，改善了关键厌氧氨氧化基因的转录，尽管减少了厌氧氨氧化丰度。动力学分析表明硝化作用比内源反硝化作用提供亚硝酸盐更快。微生物分析显示耐氧Ca. Brocadia （OTU2598 >； 97 %）持续存在。亚硝酸盐利用率的提高和基因转录水平的提高共同支持了稳定的性能，证明了双途径厌氧氨氧化作为温带地区主流处理的可行性。

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引用次数: 0

Electron pool effect: iron-quinone coordination polymer boosts anaerobic digestion by accelerating interfacial electron transfer of cathode 电子池效应：铁醌配位聚合物通过加速阴极界面电子转移促进厌氧消化

IF 9 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology

Pub Date : 2025-12-08 DOI: 10.1016/j.biortech.2025.133786

Zhihao Jiang, Qilin Yu, Zhiqiang Zhao, Ying Deng, Yaobin Zhang

Methane production in microbial electrolysis cell-anaerobic digestion (MEC-AD) systems is limited by inefficient interfacial electron transfer (IET), which depends on the slow formation of a conductive microbial biofilm. In this study, a novel material with Fe (III) ions as the metal centers and the redox-active 2,5-dihydroxy-1,4-benzoquinone (dhbq) as the organic ligand (Fe@dhbq) was designed to act as an efficient “electron pool” that mediated electron transfer between the cathode and electroactive methanogens, which helped establish an efficient IET network by accelerating the maturation of electroactive biofilms. The results demonstrated that the electron transfer capacity of the CC/Fe@dhbq biofilm increased by an order of magnitude, and the current was 4.2 times higher than the control. Correspondingly, direct electron transfer (DET) pathway was formed to increase methane production by 35 %. This work provides a new strategy for enhancing methanogenesis via DET in electro-enhanced anaerobic digestion.

微生物电解-厌氧消化（MEC-AD）系统中的甲烷产量受到低效的界面电子转移（IET）的限制，这取决于导电微生物生物膜的缓慢形成。在本研究中，设计了一种以Fe （III）离子为金属中心，以具有氧化还原活性的2,5-二羟基-1,4-苯醌（dhbq）为有机配体的新型材料（Fe@dhbq），作为高效的“电子池”，介导阴极和电活性产甲烷菌之间的电子转移，通过加速电活性生物膜的成熟，帮助建立高效的IET网络。结果表明，CC/Fe@dhbq生物膜的电子传递能力提高了一个数量级，电流比对照提高了4.2倍。形成直接电子转移（DET）途径，甲烷产量提高35% %。本研究为电强化厌氧消化中DET促进产甲烷提供了新的策略。

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引用次数: 0

Characterization and employment of novel α-ketoglutarate dependent oxygenases for efficient synthesis of antifungal alkyl citrates 新型α-酮戊二酸依赖加氧酶的表征及其在抗真菌柠檬酸烷基合成中的应用

IF 9 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology

Pub Date : 2025-12-08 DOI: 10.1016/j.biortech.2025.133787

Chunxia Xiao , Mengfei Long , Xiaoxue Sun , Dashun Yi , Md Sharuf Miah , Siyi Yang , Hang Ma , Dongsong Tian , Russell J. Cox , Xiao Zhang , Baoshun Zhang

Fungal alkyl citrates (ACs) exhibit potent antifungal activity, offering significant potential for drug development. Biosynthetic studies of sporothriolide and squalestatin S1 have shown that α-ketoglutarate dependent oxygenases (αKG OX), SpoG and MfR1/2, transform early-stage alkyl citrate precursors into diverse scaffolds through regio- and stereospecific oxidations. Here, we report two previously uncharacterized αKG OX, OryG and PaR5, which specifically hydroxylate the alkyl side chain and epoxidate the terminal alkene of alkyl itaconic acid 3, respectively. Furthermore, the catalytic mechanism and regioselectivity of PaR5 and OryG were investigated through molecular docking, site-directed mutagenesis, molecular dynamics simulation, and quantum mechanics calculation. In PaR5, the distance from the Fe(_IV)=O species to C-6 of alkyl itaconic acid 3 was greater than to C-13, which well explained the oxidation preference at the olefin position. On the contrary, the Fe(_IV)=O species are closer to C-6 than to C-13 in the OryG complex, which matches the consequence of alkyl side chain hydroxylation. By integrating Aspergillus oryzae biosynthesis, whole-cell biotransformation, and chemical workup, we developed an efficient synthetic strategy for the large-scale synthesis of antifungal sporothriolide 2 and novel epoxide 9 and lactone 10. Bioactivity assays demonstrated that sporothriolide 2 exhibits antifungal activity comparable to nystatin, while epoxide 9 and lactone 10 showed modest inhibition. Our study sheds light on the development and large-scale synthesis of novel alkyl citrates with promising antifungal activity.

真菌柠檬酸烷基酯（ACs）具有强大的抗真菌活性，为药物开发提供了巨大的潜力。孢子虫内酯和角鲨素S1的生物合成研究表明，α-酮戊二酸依赖的加氧酶（αKG OX）、SpoG和MfR1/2可以通过区域特异性和立体特异性氧化将早期的柠檬酸烷基前体转化为不同的支架。在这里，我们报道了两个先前未被表征的αKG OX， OryG和PaR5，它们分别特异性地羟基化烷基衣康酸3的烷基侧链和环氧化烷基衣康酸3的末端烯烃。此外，通过分子对接、定点诱变、分子动力学模拟和量子力学计算，研究了PaR5和OryG的催化机理和区域选择性。在PaR5中，Fe(IV)=O基团到烷基衣康酸3的C-6的距离大于到C-13的距离，这很好地解释了烯烃位置的氧化偏好。相反，在OryG配合物中，Fe(IV)=O更接近于C-6而不是C-13，这与烷基侧链羟基化的结果相匹配。通过整合米曲霉的生物合成、全细胞生物转化和化学处理，我们开发了一种高效的大规模合成抗真菌孢子内酯2和新型环氧化物9和内酯10的合成策略。生物活性分析表明，孢子虫内酯2具有与制霉菌素相当的抗真菌活性，而环氧化物9和内酯10具有适度的抑制作用。本研究为新型具有抗真菌活性的柠檬酸烷基酯的开发和大规模合成提供了思路。

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引用次数: 0

A biotechnological platform for the valorization of textile waste blend 纺织废料混纺物增值的生物技术平台。

IF 9 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology

Pub Date : 2025-12-08 DOI: 10.1016/j.biortech.2025.133781

Erika Zangelmi , Nicolò Antonelli , Jacopo La Rocca , Gianluigi Broggini , Loredano Pollegioni , Elena Rosini

Textile waste is rapidly increasing worldwide due to rising clothing consumption and production. This study presents a novel, integrated green process for degrading and valorizing blended cotton-polyethylene terephthalate (PET) textile waste into value-added chemicals through enzymatic hydrolysis and microbial fermentation. To improve degradation efficiency, specific pretreatments were developed for both cotton and PET. The process begins with mechanical ball-milling, followed by enzymatic hydrolysis of cotton using a commercial cellulases/hemicellulases cocktail at 55 °C to yield glucose. Next, the material undergoes thermal treatment at 290 °C for 15 min to prepare PET for enzymatic hydrolysis, which is then carried out at 55 °C using the S101N/F243T-ΔLCC PET-hydrolyzing enzyme variant. This approach resulted in complete conversion of cotton to glucose and ≥95 % conversion of PET to terephthalic acid (TPA) and ethylene glycol. TPA was further biotransformed with ≥90 % yield into protocatechuic acid (PCA)—a valuable aromatic compound—using an engineered E. coli strain expressing three enzymes from Comamonas sp. strain E6, namely the terephthalate dioxygenase TPADO α/β, the reductase TPADO RED, and the dehydrogenase DCDDH. The strain also utilized glucose from cotton hydrolysis as a carbon source. In total, 217.3 mg of PCA were produced from 249.2 mg of TPA (94 % yield) in 50 mL of a 30 mM substrate solution. From 1 g of polycotton fabric (30 % cotton, 70 % PET), approximately 500 mg of PCA (92 % yield) were generated. This integrated biorefinery process highlights a promising strategy for sustainable textile waste valorization.

由于服装消费和生产的增加，纺织品废料在世界范围内迅速增加。本研究提出了一种新的、综合的绿色工艺，通过酶水解和微生物发酵将混纺棉-聚对苯二甲酸乙二醇酯（PET）纺织废料降解和增值为增值化学品。为提高降解效率，对棉花和PET进行了特异性预处理。该工艺从机械球磨开始，然后使用商用纤维素酶/半纤维素酶混合物在55 °C下对棉花进行酶水解以产生葡萄糖。接下来，材料在290 °C下热处理15 min，以制备用于酶解的PET，然后使用S101N/F243T-ΔLCC PET水解酶变体在55 °C下进行酶解。该方法使棉花完全转化为葡萄糖，PET转化为对苯二甲酸（TPA）和乙二醇的转化率≥95% %。TPA进一步生物转化为原儿茶酸(PCA)-一种有价值的芳香化合物-利用工程大肠杆菌菌株表达三种酶，分别是对苯二甲酸双加氧酶TPADO α/β，还原酶TPADO RED和脱氢酶DCDDH，产率≥90 %。该菌株还利用棉花水解产生的葡萄糖作为碳源。在50 mL的30 mM底物溶液中，249.2 mg TPA（收率为94% %）共生产217.3 mg PCA。从1 g的涤棉织物（30 %棉，70 % PET）中，产生约500 mg PCA（产率92 %）。这种综合的生物精炼工艺突出了可持续纺织废物增值的有前途的战略。

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引用次数: 0

Synergistic division of labor in a bacterial consortium for enhanced phenanthrene mineralization under cadmium stress: mechanisms of degradation-detoxification coordination 镉胁迫下细菌联合体中加强菲矿化的协同分工：降解-解毒协调机制

IF 11.4 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology

Pub Date : 2025-12-07 DOI: 10.1016/j.biortech.2025.133782

Tianzuo Cheng, Pengyu Zhou, Mingle Zhang, Tianyin Huang, Bingdang Wu, Jinlong Zhuang, Bin Wang, Xiaoyi Xu

The remediation of co-contamination by polycyclic aromatic hydrocarbons (PAHs) and heavy metals poses a significant challenge. Although microbial consortia present a promising approach, their synergistic mechanisms under stress conditions are not fully understood. To address this gap, we constructed a functionally specialized bacterial consortium (KZ) by assembling Klebsiella sp. CW-D3T and Arthrobacter sp. SZ-3, which synergistically enhanced phenanthrene (PHE) degradation and mineralization under cadmium stress (25 mg/L Cd2+), outperforming monocultures by 1.2–1.9-fold. Through biomass-normalized enzyme activity assays, we uncovered a structured division of labor: SZ-3 exhibited superior upstream catalytic activity (50 % higher 2H1N conversion), while CW-D3T dominated downstream mineralization (>80 % contribution). Mechanistic investigations via metagenomics revealed that CW-D3T utilized high-expression efflux pumps (ZntA/zinT) and antioxidant genes (yhcN) to mitigate cadmium toxicity, whereas SZ-3 employed the frnE-mediated oxidative stress response and limited Cd2+ uptake via mntH. This study elucidates a synergistic mechanism for concurrent PAH degradation and heavy metal detoxification, offering a novel bioresource for remediating co-contaminated environments.

多环芳烃（PAHs）与重金属共污染的修复是一个重大挑战。虽然微生物联合体提出了一种很有前途的方法，但它们在逆境条件下的协同机制尚不完全清楚。为了解决这一空白，我们通过组装克雷伯菌sp. CW-D3T和节杆菌sp. SZ-3构建了一个功能特化的细菌联合体（KZ），它们在镉胁迫（25 mg/L Cd2+）下协同促进了菲（PHE）的降解和矿化，比单一培养的效果高1.2 - 1.9倍。通过生物量归一化酶活性测定，我们发现了一个结构化的分工：SZ-3具有优越的上游催化活性（2H1N转化率提高50% %），而CW-D3T主导下游矿化（贡献>； 80% %）。宏基因组学的机制研究表明，CW-D3T通过高表达外排泵（ZntA/zinT）和抗氧化基因（yhcN）来减轻镉毒性，而SZ-3通过frne介导的氧化应激反应和通过mntH限制Cd2+的摄取。本研究阐明了多环芳烃同时降解和重金属解毒的协同机制，为修复共污染环境提供了新的生物资源。

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引用次数: 0

Nanocellulose hydrogel-based living materials: enhancing microbial biotransformation of lignocellulosic biomass 纳米纤维素水凝胶基生物材料：增强木质纤维素生物质的微生物转化

IF 11.4 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology

Pub Date : 2025-12-07 DOI: 10.1016/j.biortech.2025.133783

Xueyu Tang, Wei Hu, Zhiguo Wang, Yimin Fan, Yong Xu, Qiang Yong, Xin Li, Xia Hua

引用次数: 0

Treatment of ultra-high-strength compost leachate using an anaerobic biomass biofilm reactor 厌氧生物质生物膜反应器处理超高强度堆肥渗滤液

IF 11.4 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology

Pub Date : 2025-12-04 DOI: 10.1016/j.biortech.2025.133774

Anusree Nalladiyil, Himanshu Kumar Khuntia, H.N. Chanakya, G.L. Sivakumar Babu

Leachate produced during the composting of the organic fraction of municipal solid waste (OFMSW) is highly concentrated and acidic (chemical oxygen demand (COD) -125 g/L, pH 3-5). Its recalcitrant nature necessitates long hydraulic retention times for effective digestion, which, in turn, leads to high organic loads and, consequently, large reactor footprints. This study evaluated the treatment performance, bioenergy potential, and microbial ecology of the Anaerobic Biomass Biofilm Reactor (ABBR) for ultra-high strength leachate treatment. The reactor employed lignocellulosic wastes such as coir, ridge gourd, and dried acacia leaves as natural biofilm supports. Operated over 180 days with a gradually increasing organic loading rate from 1.1 to 11.2 kg COD/m3/d, the reactor achieved 92.9 % COD removal and a methane yield of 0.357 NL/g COD removed at the maximum loading rate. Moreover, the reactor also exhibited exceptionally high space utilization efficiency (3.5-4 L CH4/L/d), highlighting its enhanced volumetric productivity and effectiveness in treating high-strength leachate. Metagenomic analysis revealed a diverse microbial community, with Methanospirillum (3 %) and Methanosaeta (2.6 %) identified as dominant archaea contributing to methanogenesis. The high moisture content of OFMSW, coupled with tropical climatic conditions, leads to rapid fermentation and the generation of large volumes of leachate. Therefore, the ABBR represents a sustainable and high-rate alternative to conventional anaerobic systems, enabling efficient leachate treatment and enhanced bioenergy recovery in windrow composting facilities.

城市生活垃圾（OFMSW）有机部分堆肥过程中产生的渗滤液高浓度且呈酸性（化学需氧量(COD) -125 g/L， pH值3-5）。它的顽固性需要很长的水力滞留时间才能有效消化，这反过来又导致高有机负荷，从而导致大的反应器占地面积。本研究对厌氧生物质生物膜反应器（ABBR）处理超高强度渗滤液的性能、生物能源潜力和微生物生态学进行了评价。该反应器采用木质纤维素废弃物，如椰子、冬瓜和干燥的金合欢叶作为天然生物膜载体。反应器运行180 天，有机负荷率从1.1 kg /m3/d逐渐提高到11.2 kg /m3/d，最大负荷率下COD去除率达到92.9 %，甲烷产率为0.357 NL/g。此外，该反应器还表现出极高的空间利用效率（3.5-4 L CH4/L/d），突出了其提高的体积生产率和处理高强度渗滤液的有效性。宏基因组分析显示微生物群落多样性，其中Methanospirillum（3 %）和Methanosaeta（2.6 %）被确定为主导产甲烷的古菌。OFMSW的高水分含量，加上热带气候条件，导致快速发酵和产生大量的渗滤液。因此，ABBR代表了传统厌氧系统的可持续和高速率替代方案，实现了高效的渗滤液处理，并增强了窗口堆肥设施的生物能源回收。

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引用次数: 0

Unveiling pyrolysis mechanism of furfural: a theoretical and kinetic study 揭示糠醛热解机理：理论与动力学研究。

IF 9 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology

Pub Date : 2025-12-04 DOI: 10.1016/j.biortech.2025.133747

Yuqiang Li , Jiaxin Sun , Shoulong Lin , Yong Chen

As a core platform molecule in biomass conversion, furfural requires accurate prediction of its pyrolysis behavior for the practical application of furan-based alternative fuels. However, existing pyrolysis models are inadequate for describing its thermal decomposition, as many kinetic parameters estimated via the analogical method introduce significant uncertainty. This study employs quantum chemical calculations based on Transition State Theory (TST) and Rice Ramsperger Kassel Marcus (RRKM) theory to systematically investigate hydrogen-abstraction, hydrogen-addition, and unimolecular reactions in furfural pyrolysis, with reaction rates calculated over 500–2500 K. Results show that the analogical method overestimates the rates of hydrogen-abstraction and hydrogen-addition reactions at low temperatures. Simulation validation was conducted using a Perfectly Stirred Reactor (PSR). Mole fraction comparison analysis confirms the improved model more accurately reproduces the variation trends and magnitudes of experimental data. Sensitivity analysis indicates that the modified model corrects the potential overestimation of the contribution of addition pathways in the original model.

糠醛作为生物质转化的核心平台分子，需要对其热解行为进行准确预测，才能实现基于糠醛的替代燃料的实际应用。然而，现有的热解模型不足以描述其热分解，因为通过类比方法估计的许多动力学参数引入了很大的不确定性。本研究采用基于过渡态理论（TST）和Rice Ramsperger Kassel Marcus （RRKM）理论的量子化学计算，系统研究了糠醛热解过程中的抽氢、加氢和单分子反应，反应速率在500-2500 K以上。结果表明，类比法过高估计了低温下吸氢和加氢反应的速率。采用完全搅拌反应器（PSR）进行了仿真验证。摩尔分数对比分析证实，改进模型更准确地再现了实验数据的变化趋势和幅度。灵敏度分析表明，修正后的模型纠正了原模型中对附加路径贡献的潜在高估。

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