Bioresource Technology最新文献_第3页

A biotechnological platform for the valorization of textile waste blend. 纺织废料混纺物增值的生物技术平台。

IF 9 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology

Pub Date : 2025-12-08 DOI: 10.1016/j.biortech.2025.133781

Erika Zangelmi, Nicolò Antonelli, Jacopo La Rocca, Gianluigi Broggini, Loredano Pollegioni, Elena Rosini

Textile waste is rapidly increasing worldwide due to rising clothing consumption and production. This study presents a novel, integrated green process for degrading and valorizing blended cotton-polyethylene terephthalate (PET) textile waste into value-added chemicals through enzymatic hydrolysis and microbial fermentation. To improve degradation efficiency, specific pretreatments were developed for both cotton and PET. The process begins with mechanical ball-milling, followed by enzymatic hydrolysis of cotton using a commercial cellulases/hemicellulases cocktail at 55 °C to yield glucose. Next, the material undergoes thermal treatment at 290 °C for 15 min to prepare PET for enzymatic hydrolysis, which is then carried out at 55 °C using the S101N/F243T-ΔLCC PET-hydrolyzing enzyme variant. This approach resulted in complete conversion of cotton to glucose and ≥95 % conversion of PET to terephthalic acid (TPA) and ethylene glycol. TPA was further biotransformed with ≥90 % yield into protocatechuic acid (PCA)-a valuable aromatic compound-using an engineered E. coli strain expressing three enzymes from Comamonas sp. strain E6, namely the terephthalate dioxygenase TPADO α/β, the reductase TPADO RED, and the dehydrogenase DCDDH. The strain also utilized glucose from cotton hydrolysis as a carbon source. In total, 217.3 mg of PCA were produced from 249.2 mg of TPA (94 % yield) in 50 mL of a 30 mM substrate solution. From 1 g of polycotton fabric (30 % cotton, 70 % PET), approximately 500 mg of PCA (92 % yield) were generated. This integrated biorefinery process highlights a promising strategy for sustainable textile waste valorization.

由于服装消费和生产的增加，纺织品废料在世界范围内迅速增加。本研究提出了一种新的、综合的绿色工艺，通过酶水解和微生物发酵将混纺棉-聚对苯二甲酸乙二醇酯（PET）纺织废料降解和增值为增值化学品。为提高降解效率，对棉花和PET进行了特异性预处理。该工艺从机械球磨开始，然后使用商用纤维素酶/半纤维素酶混合物在55 °C下对棉花进行酶水解以产生葡萄糖。接下来，材料在290 °C下热处理15 min，以制备用于酶解的PET，然后使用S101N/F243T-ΔLCC PET水解酶变体在55 °C下进行酶解。该方法使棉花完全转化为葡萄糖，PET转化为对苯二甲酸（TPA）和乙二醇的转化率≥95% %。TPA进一步生物转化为原儿茶酸(PCA)-一种有价值的芳香化合物-利用工程大肠杆菌菌株表达三种酶，分别是对苯二甲酸双加氧酶TPADO α/β，还原酶TPADO RED和脱氢酶DCDDH，产率≥90 %。该菌株还利用棉花水解产生的葡萄糖作为碳源。在50 mL的30 mM底物溶液中，249.2 mg TPA（收率为94% %）共生产217.3 mg PCA。从1 g的涤棉织物（30 %棉，70 % PET）中，产生约500 mg PCA（产率92 %）。这种综合的生物精炼工艺突出了可持续纺织废物增值的有前途的战略。

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引用次数: 0

Synergistic division of labor in a bacterial consortium for enhanced phenanthrene mineralization under cadmium stress: mechanisms of degradation-detoxification coordination 镉胁迫下细菌联合体中加强菲矿化的协同分工：降解-解毒协调机制

IF 11.4 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology

Pub Date : 2025-12-07 DOI: 10.1016/j.biortech.2025.133782

Tianzuo Cheng, Pengyu Zhou, Mingle Zhang, Tianyin Huang, Bingdang Wu, Jinlong Zhuang, Bin Wang, Xiaoyi Xu

The remediation of co-contamination by polycyclic aromatic hydrocarbons (PAHs) and heavy metals poses a significant challenge. Although microbial consortia present a promising approach, their synergistic mechanisms under stress conditions are not fully understood. To address this gap, we constructed a functionally specialized bacterial consortium (KZ) by assembling Klebsiella sp. CW-D3T and Arthrobacter sp. SZ-3, which synergistically enhanced phenanthrene (PHE) degradation and mineralization under cadmium stress (25 mg/L Cd2+), outperforming monocultures by 1.2–1.9-fold. Through biomass-normalized enzyme activity assays, we uncovered a structured division of labor: SZ-3 exhibited superior upstream catalytic activity (50 % higher 2H1N conversion), while CW-D3T dominated downstream mineralization (>80 % contribution). Mechanistic investigations via metagenomics revealed that CW-D3T utilized high-expression efflux pumps (ZntA/zinT) and antioxidant genes (yhcN) to mitigate cadmium toxicity, whereas SZ-3 employed the frnE-mediated oxidative stress response and limited Cd2+ uptake via mntH. This study elucidates a synergistic mechanism for concurrent PAH degradation and heavy metal detoxification, offering a novel bioresource for remediating co-contaminated environments.

多环芳烃（PAHs）与重金属共污染的修复是一个重大挑战。虽然微生物联合体提出了一种很有前途的方法，但它们在逆境条件下的协同机制尚不完全清楚。为了解决这一空白，我们通过组装克雷伯菌sp. CW-D3T和节杆菌sp. SZ-3构建了一个功能特化的细菌联合体（KZ），它们在镉胁迫（25 mg/L Cd2+）下协同促进了菲（PHE）的降解和矿化，比单一培养的效果高1.2 - 1.9倍。通过生物量归一化酶活性测定，我们发现了一个结构化的分工：SZ-3具有优越的上游催化活性（2H1N转化率提高50% %），而CW-D3T主导下游矿化（贡献>； 80% %）。宏基因组学的机制研究表明，CW-D3T通过高表达外排泵（ZntA/zinT）和抗氧化基因（yhcN）来减轻镉毒性，而SZ-3通过frne介导的氧化应激反应和通过mntH限制Cd2+的摄取。本研究阐明了多环芳烃同时降解和重金属解毒的协同机制，为修复共污染环境提供了新的生物资源。

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引用次数: 0

Nanocellulose hydrogel-based living materials: enhancing microbial biotransformation of lignocellulosic biomass 纳米纤维素水凝胶基生物材料：增强木质纤维素生物质的微生物转化

IF 11.4 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology

Pub Date : 2025-12-07 DOI: 10.1016/j.biortech.2025.133783

Xueyu Tang, Wei Hu, Zhiguo Wang, Yimin Fan, Yong Xu, Qiang Yong, Xin Li, Xia Hua

引用次数: 0

Treatment of ultra-high-strength compost leachate using an anaerobic biomass biofilm reactor 厌氧生物质生物膜反应器处理超高强度堆肥渗滤液

IF 11.4 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology

Pub Date : 2025-12-04 DOI: 10.1016/j.biortech.2025.133774

Anusree Nalladiyil, Himanshu Kumar Khuntia, H.N. Chanakya, G.L. Sivakumar Babu

Leachate produced during the composting of the organic fraction of municipal solid waste (OFMSW) is highly concentrated and acidic (chemical oxygen demand (COD) -125 g/L, pH 3-5). Its recalcitrant nature necessitates long hydraulic retention times for effective digestion, which, in turn, leads to high organic loads and, consequently, large reactor footprints. This study evaluated the treatment performance, bioenergy potential, and microbial ecology of the Anaerobic Biomass Biofilm Reactor (ABBR) for ultra-high strength leachate treatment. The reactor employed lignocellulosic wastes such as coir, ridge gourd, and dried acacia leaves as natural biofilm supports. Operated over 180 days with a gradually increasing organic loading rate from 1.1 to 11.2 kg COD/m3/d, the reactor achieved 92.9 % COD removal and a methane yield of 0.357 NL/g COD removed at the maximum loading rate. Moreover, the reactor also exhibited exceptionally high space utilization efficiency (3.5-4 L CH4/L/d), highlighting its enhanced volumetric productivity and effectiveness in treating high-strength leachate. Metagenomic analysis revealed a diverse microbial community, with Methanospirillum (3 %) and Methanosaeta (2.6 %) identified as dominant archaea contributing to methanogenesis. The high moisture content of OFMSW, coupled with tropical climatic conditions, leads to rapid fermentation and the generation of large volumes of leachate. Therefore, the ABBR represents a sustainable and high-rate alternative to conventional anaerobic systems, enabling efficient leachate treatment and enhanced bioenergy recovery in windrow composting facilities.

城市生活垃圾（OFMSW）有机部分堆肥过程中产生的渗滤液高浓度且呈酸性（化学需氧量(COD) -125 g/L， pH值3-5）。它的顽固性需要很长的水力滞留时间才能有效消化，这反过来又导致高有机负荷，从而导致大的反应器占地面积。本研究对厌氧生物质生物膜反应器（ABBR）处理超高强度渗滤液的性能、生物能源潜力和微生物生态学进行了评价。该反应器采用木质纤维素废弃物，如椰子、冬瓜和干燥的金合欢叶作为天然生物膜载体。反应器运行180 天，有机负荷率从1.1 kg /m3/d逐渐提高到11.2 kg /m3/d，最大负荷率下COD去除率达到92.9 %，甲烷产率为0.357 NL/g。此外，该反应器还表现出极高的空间利用效率（3.5-4 L CH4/L/d），突出了其提高的体积生产率和处理高强度渗滤液的有效性。宏基因组分析显示微生物群落多样性，其中Methanospirillum（3 %）和Methanosaeta（2.6 %）被确定为主导产甲烷的古菌。OFMSW的高水分含量，加上热带气候条件，导致快速发酵和产生大量的渗滤液。因此，ABBR代表了传统厌氧系统的可持续和高速率替代方案，实现了高效的渗滤液处理，并增强了窗口堆肥设施的生物能源回收。

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引用次数: 0

Unveiling pyrolysis mechanism of furfural: a theoretical and kinetic study 揭示糠醛热解机理：理论与动力学研究。

IF 9 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology

Pub Date : 2025-12-04 DOI: 10.1016/j.biortech.2025.133747

Yuqiang Li , Jiaxin Sun , Shoulong Lin , Yong Chen

As a core platform molecule in biomass conversion, furfural requires accurate prediction of its pyrolysis behavior for the practical application of furan-based alternative fuels. However, existing pyrolysis models are inadequate for describing its thermal decomposition, as many kinetic parameters estimated via the analogical method introduce significant uncertainty. This study employs quantum chemical calculations based on Transition State Theory (TST) and Rice Ramsperger Kassel Marcus (RRKM) theory to systematically investigate hydrogen-abstraction, hydrogen-addition, and unimolecular reactions in furfural pyrolysis, with reaction rates calculated over 500–2500 K. Results show that the analogical method overestimates the rates of hydrogen-abstraction and hydrogen-addition reactions at low temperatures. Simulation validation was conducted using a Perfectly Stirred Reactor (PSR). Mole fraction comparison analysis confirms the improved model more accurately reproduces the variation trends and magnitudes of experimental data. Sensitivity analysis indicates that the modified model corrects the potential overestimation of the contribution of addition pathways in the original model.

糠醛作为生物质转化的核心平台分子，需要对其热解行为进行准确预测，才能实现基于糠醛的替代燃料的实际应用。然而，现有的热解模型不足以描述其热分解，因为通过类比方法估计的许多动力学参数引入了很大的不确定性。本研究采用基于过渡态理论（TST）和Rice Ramsperger Kassel Marcus （RRKM）理论的量子化学计算，系统研究了糠醛热解过程中的抽氢、加氢和单分子反应，反应速率在500-2500 K以上。结果表明，类比法过高估计了低温下吸氢和加氢反应的速率。采用完全搅拌反应器（PSR）进行了仿真验证。摩尔分数对比分析证实，改进模型更准确地再现了实验数据的变化趋势和幅度。灵敏度分析表明，修正后的模型纠正了原模型中对附加路径贡献的潜在高估。

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引用次数: 0

Perfluorooctane sulfonate (PFOS) inhibits methane production during sludge anaerobic digestion by breaking the carbon-transfer bridge between methanogenesis and acidogenesis 全氟辛烷磺酸（PFOS）通过破坏甲烷生成和酸生成之间的碳传递桥梁，抑制污泥厌氧消化过程中的甲烷生成

IF 11.4 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology

Pub Date : 2025-12-04 DOI: 10.1016/j.biortech.2025.133775

Zhaobin Liu, Yijing Gao, Jiayi Wang, Xinyu Jing, Xue Chen, Weijie Hu, Xueqin Lu, Meng Liu, Xiaosong He, Gopalakrishnan Kumar, Guangyin Zhen

Per- or polyfluoroalkyl compounds (PFASs) are recognized as emerging contaminant, with perfluorooctane sulfonate (PFOS) being one of the most extensively utilized PFASs due to its great chemical stability. However, knowledge of the bio-chemical behavior, the toxicity of PFOS and its mechanisms of interfacial binding to microorganisms remain inadequately validated. In this study, the biotoxicity of PFOS and its molecular interfacial adsorption mechanism in anaerobic digestion were investigated. The results showed that the tightly bound EPS (TB-EPS) of anaerobic microorganisms could defend against the biotoxicity of PFOS to some extent by physical adsorption and chemical binding, the exposure to PFOS might produce a greater disturbance to methanogenic archaea. With the increase of PFOS, acid-producing bacteria (APB) and methanogenic archaea showed different resistance to PFOS, suppressing cumulative methane production by up to 91.64 %. On the contrary, APBs were more tolerant, and fatty acids accumulated up to 2194.27 mg/L. Metagenomics analysis further confirmed that functional genes associated with fatty acid biosynthesis (fas, FAS2, fabK, etc.) were significantly enriched (approximately 85.29 %) whereas the relative abundance of genes associated with methanogenesis (acs, comA, mcrB, etc.) were decreased (up to 65.96 %). Molecular docking results suggested a potential route for PFOS cellular entry, as it was observed to bind to the substrate-binding protein of the ATP-binding cassette (ABC) transporter and interact with key functional enzymes, which led to the inhibition of methanogens. This study provides novel insights into the molecular blocking mechanism by which PFOS disrupts carbon metabolic flux through the selective inhibition of methanogenic archaea, rather than through a general suppression of acidogenic bacteria.

全氟烷基或多氟烷基化合物（PFASs）是公认的新兴污染物，全氟辛烷磺酸（PFOS）因其具有很强的化学稳定性而成为使用最广泛的全氟烷基化合物之一。然而，对全氟辛烷磺酸的生化行为、毒性及其与微生物的界面结合机制的了解仍然不够充分。研究了全氟辛烷磺酸的生物毒性及其在厌氧消化中的分子界面吸附机理。结果表明，厌氧微生物紧密结合的EPS （TB-EPS）能够通过物理吸附和化学结合在一定程度上抵御全氟辛烷磺酸的生物毒性，暴露于全氟辛烷磺酸可能对产甲烷古菌产生较大的干扰。随着PFOS浓度的升高，产酸菌（APB）和产甲烷古菌对PFOS表现出不同的抗性，对累积产甲烷的抑制幅度高达91.64 %。相反，APBs的耐受性更强，脂肪酸累积量高达2194.27 mg/L。宏基因组学分析进一步证实，与脂肪酸生物合成相关的功能基因（fas, FAS2， fabK等）显著富集（约85.29 %），而与甲烷生成相关的基因（acs, comA， mcrB等）的相对丰度降低（高达65.96 %）。分子对接结果提示了PFOS进入细胞的潜在途径，因为观察到PFOS与atp结合盒（ABC）转运体的底物结合蛋白结合，并与关键功能酶相互作用，从而抑制产甲烷菌。这项研究为全氟辛烷磺酸通过选择性抑制产甲烷古菌而不是通过普遍抑制产酸细菌来破坏碳代谢通量的分子阻断机制提供了新的见解。

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引用次数: 0

The role of artificial intelligence in biomass pyrolysis: Advancing predictive modelling and mechanistic understanding through machine learning − A comprehensive review 人工智能在生物质热解中的作用：通过机器学习推进预测建模和机制理解-综合综述

IF 11.4 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology

Pub Date : 2025-12-04 DOI: 10.1016/j.biortech.2025.133777

Luchen Yang, Brahim El Allaoui, Xinyun Wu, Tao Wu, Wai Siong Chai, Cheng Heng Pang

Biomass pyrolysis is a promising thermochemical pathway for producing renewable fuels and chemicals, yet its optimization remains challenging due to the complex interactions among feedstock properties, operating conditions, and reactor configurations. Artificial intelligence (AI), particularly machine learning (ML), is emerging as a powerful tool to improve predictive modelling and mechanistic understanding of pyrolysis. This review critically examines four pyrolysis regimes (slow, intermediate, fast, and flash) and advanced strategies such as co-pyrolysis, catalytic enhancement, and microwave heating, before discussing how ML algorithms are reshaping data-driven modelling. Supervised models, including random forests (RF), extreme gradient boosting (XGBoost), and artificial neural networks (ANNs), have achieved high accuracy in predicting product yields, while unsupervised and reinforcement learning approaches reveal hidden trends and enable adaptive process control. Furthermore, explainable AI frameworks (XAI), such as Shapley Additive Explanations (SHAP) and partial dependence plots (PDP), provide mechanistic insights that bridge data with reaction pathways. By combining AI with reactor optimization and mechanistic explanation, this field offers a pathway to efficient and scalable pyrolysis systems. Finally, we outline research gaps and future directions, emphasising the need for interpretable and generalisable models that can guide large-scale deployment.

生物质热解是一种很有前途的生产可再生燃料和化学品的热化学途径，但由于原料性质、操作条件和反应器配置之间复杂的相互作用，其优化仍然具有挑战性。人工智能（AI），特别是机器学习（ML），正在成为改进预测建模和对热解机理理解的有力工具。在讨论机器学习算法如何重塑数据驱动建模之前，本文对四种热解方式（慢速、中间、快速和快速）和高级策略（如共热解、催化增强和微波加热）进行了严格的研究。有监督模型，包括随机森林（RF）、极端梯度增强（XGBoost）和人工神经网络（ann），在预测产品产量方面取得了很高的准确性，而无监督和强化学习方法揭示了隐藏的趋势，并实现了自适应过程控制。此外，可解释的人工智能框架（XAI），如Shapley加性解释（SHAP）和部分依赖图（PDP），提供了将数据与反应途径联系起来的机制见解。通过将人工智能与反应器优化和机理解释相结合，该领域为高效、可扩展的热解系统提供了一条途径。最后，我们概述了研究差距和未来的方向，强调需要可解释和可推广的模型，以指导大规模部署。

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引用次数: 0

Untreated molasses efficiently Enhances 2,3-Butanediol synthesis using engineered Aureobasidium melanogenum P8AC-4 未经处理的糖蜜有效地提高了2,3-丁二醇的合成，利用工程的黑素短毛霉P8AC-4

IF 9 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology

Pub Date : 2025-12-04 DOI: 10.1016/j.biortech.2025.133776

Kai Wang , Xiancheng Wang , Enpeng Zhang , Haixia Yang , Qinggele Caiyin , Mingzhang Wen , Jianjun Qiao

2,3-Butanediol, an important platform chemical, possesses extensive applications in chemical engineering, pharmaceuticals, and materials science. To address the challenges of resource depletion and environmental impact associated with conventional petroleum-based synthesis, this study focuses on the sustainable production of 2,3-BDO via microbial fermentation. Aureobasidium melanogenum TSYW-58 was employed as the chassis for metabolic engineering. In previous work, genes responsible for the biosynthesis of pullulan, polymalic acid, melanin, and liamocin were deleted, yielding strain PPLM-8, characterized by high gluconic acid titer and reduced by-product formation. Building upon this platform, genes encoding α-acetolactate synthase and α-acetolactate decarboxylase from Bacillus subtilis were heterologously expressed, along with the endogenous butanediol dehydrogenase, leading to the successful construction of strain P8AC-4. This strain is capable of efficient 2,3-BDO production with minimal by-products. Shake-flask fermentation yielded 43.21 ± 1.23 g/L of 2,3-BDO, with a stereoisomer composition of 77.07 % meso- and 22.93 % (R,R)-isomer. Fermentation kinetic analysis in a 5 L bioreactor revealed that 2,3-BDO biosynthesis is tightly coupled with the logarithmic growth phase of the microorganism. By leveraging this metabolic trait, growth-inhibiting components present in untreated straw hydrolysate and molasses were employed to modulate fungal growth rates. Notably, cultivation with untreated molasses as the carbon source effectively extended the product synthesis phase, achieving a 2,3-BDO concentration of 110.37 ± 4.43 g/L in batch culture, representing a 155.42 % increase compared to that obtained with the glucose-based medium. These findings offer an efficient and cost-effective biomanufacturing strategy for the industrial-scale production of 2,3-BDO, contributing to the advancement of sustainable chemical synthesis.

2,3-丁二醇是一种重要的平台化学品，在化学工程、制药和材料科学等领域有着广泛的应用。为了解决与传统石油基合成相关的资源枯竭和环境影响的挑战，本研究侧重于通过微生物发酵可持续生产2,3- bdo。以黑素小孢子菌TSYW-58为基础进行代谢工程。在之前的研究中，我们删除了负责普鲁兰、聚苹果酸、黑色素和利亚霉素生物合成的基因，产生了菌株PPLM-8，其特点是葡萄糖酸滴度高，副产物形成减少。在此平台上，将枯草芽孢杆菌α-乙酰乳酸合成酶和α-乙酰乳酸脱羧酶基因与内源性丁二醇脱氢酶进行异源表达，成功构建菌株P8AC-4。该菌株能够以最少的副产物高效生产2,3- bdo。摇瓶发酵产2,3- bdo 43.21±1.23 g/L，立体异构体组成为77.07 %中位异构体和22.93 % （R，R）异构体。在5l生物反应器中发酵动力学分析表明，2,3- bdo的生物合成与微生物的对数生长阶段紧密耦合。利用这一代谢特性，未经处理的秸秆水解物和糖蜜中存在的生长抑制成分被用来调节真菌的生长速率。值得注意的是，以未经处理的糖蜜为碳源的培养有效地延长了产物合成阶段，在批量培养中达到2,3- bdo浓度为110.37±4.43 g/L，与葡萄糖基培养基相比，增加了155.42 %。这些发现为2,3- bdo的工业规模生产提供了一种高效且具有成本效益的生物制造策略，有助于推进可持续化学合成。

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引用次数: 0

Enhanced azithromycin removal in microalgae-microbial fuel cells: comparative analysis of anode and cathode configurations 微藻-微生物燃料电池对阿奇霉素的去除：阳极和阴极配置的比较分析。

IF 9 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology

Pub Date : 2025-12-03 DOI: 10.1016/j.biortech.2025.133771

Jesna Fathima , P.R. Sreekutty , Pritha Chatterjee

Azithromycin (AZ), a broad-spectrum antibiotic, is commonly found in aquatic habitats. This study investigated two microalgae-microbial fuel cell (m-MFC) configurations, A-AZ-MFC (AZ in the anode) and C-AZ-MFC (AZ in the cathode), which were run in fed-batch mode under open- and closed-circuit conditions (10–200 mg/L AZ). AZ removal increased from 43 % in an open circuit to 83 % when the circuit was closed in A-AZ-MFC and from 68 % to 84 % in C-AZ-MFC. While both A-AZ-MFC and C-AZ-MFC achieved comparable AZ degradation (83–84 %), A-AZ-MFC demonstrated superior electrochemical output (Power density: 275 mW/m³; Net energy recovery: 0.11 kWh/kg COD; Coulombic efficiency: 26 %) and higher microbial tolerance (IC₅₀ = 77.02 mg/L), indicating effective electron transfer and steady biofilm activity. Both designs achieved successful detoxification, as evidenced by comparable transformation product profiles and lower effluent toxicity. These findings demonstrate m-MFCs, especially anode-optimized systems, as sustainable platforms for the removal of antibiotics and the production of bioenergy.

阿奇霉素（AZ）是一种广谱抗生素，常见于水生生境。本研究研究了两种微藻-微生物燃料电池（m-MFC）配置，A-AZ-MFC（阳极AZ）和C-AZ-MFC（阴极AZ），在开路和闭路条件下（10-200 mg/L AZ）以进料间歇模式运行。在A-AZ-MFC中，AZ去除率从开路时的43 %增加到83 %，在C-AZ-MFC中从68 %增加到84 %。虽然A-AZ-MFC和C-AZ-MFC对AZ的降解效果相当（83-84 %），但A-AZ-MFC表现出更好的电化学输出（功率密度：275 mW/m3；净能量回收率：0.11 kWh/kg COD；库仑效率：26 %）和更高的微生物耐受性（IC50 = 77.02 mg/L），表明有效的电子转移和稳定的生物膜活性。两种设计都实现了成功的解毒，如可比的转化产品概况和较低的出水毒性所证明的那样。这些发现表明，m- mfc，特别是阳极优化系统，是去除抗生素和生产生物能源的可持续平台。

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引用次数: 0

Using the hydrolysate of Chinese medicine residues as nutrient source improves the performance of oil-producing algae: Phenomena and mechanistic elucidation. 以中药残渣水解液为营养源可提高产油藻类的生产性能：现象及机理解析。

IF 9 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology

Pub Date : 2025-12-03 DOI: 10.1016/j.biortech.2025.133737

Shiyi Chen, Ruixin Guo, Zixuan Zheng, Wenling Chen, Cheng Ma, Yanhua Liu, Jianqiu Chen, Jian Ke

The development of microalgae-based biofuels has long been a research focus for achieving green and sustainable development. Chinese medicine residues, an abundant organic waste rich in cellulose, hemicellulose, lignin, proteins, polysaccharides, and microminerals, offer scalable feedstock. Here, their hydrolysate (HCMR) was used as an additional nutrient source for cultivating oil-producing algae. The hydrolysate promotes the growth and productivity of oil-producing algae such as Chlorococcum sp., Tribonema aequale, and Scenedesmus sp., while also indicating its feasibility for scaling up Scenedesmus sp. cultivation. The HCMR addition increased biomass by 1.58-fold and fatty‑acid content by 1.27‑fold relative to BG11 medium. Analysis of the fatty acid composition in Scenedesmus sp. confirmed that the addition of hydrolysate from Chinese medicine residues significantly increased the proportions of high-quality fatty acids, including palmitoleic acid (C16:1) and oleic acid (C18:1), while reducing the proportions of linoleic acid (C18:2) and α-linolenic acid (C18:3), thereby improving the cetane number and oxidative stability of the biodiesel. Metabolomics analysis reveals potential regulatory pathways by which HCMR enhances lipid accumulation in Scenedesmus sp., implicating transamination in glutamate metabolism and offering a sustainable route for utilizing similar organic waste as an additional source of nutrients in oil-producing microalgae cultivation.

微藻生物燃料的开发一直是实现绿色可持续发展的研究热点。中药残渣是一种富含纤维素、半纤维素、木质素、蛋白质、多糖和微量矿物质的有机废物，是可扩展的原料。在这里，它们的水解物（HCMR）被用作培养产油藻类的额外营养来源。该水解液对产油藻类如chlorcoccum sp.、Tribonema aequale和Scenedesmus sp.的生长和产量有促进作用，同时也表明了扩大Scenedesmus sp.养殖的可行性。与BG11培养基相比，添加HCMR可使生物量提高1.58倍，脂肪酸含量提高1.27倍。通过对Scenedesmus sp.脂肪酸组成的分析，证实中药残液水解液的加入显著提高了棕榈油酸（C16:1）和油酸（C18:1）等优质脂肪酸的比例，同时降低了亚油酸（C18:2）和α-亚麻酸（C18:3）的比例，从而提高了十六烷值和氧化稳定性。代谢组学分析揭示了HCMR促进Scenedesmus sp.脂质积累的潜在调控途径，暗示谷氨酸代谢中的转氨酶作用，并为利用类似的有机废物作为产油微藻培养的额外营养来源提供了可持续的途径。

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