Carbohydrate Polymers最新文献

英文中文

Nano Sim@ZIF8@PDA modified injectable temperature sensitive nanocomposite hydrogel for photothermal/drug therapy for peri-implantitis

IF 10.7 1区化学 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymers

Pub Date : 2025-01-28 DOI: 10.1016/j.carbpol.2025.123327

Dingkun Liu , Jinbing Chen , Linjuan Gao , Xing Chen , Liujun Lin , Yuan Liu , Xia Wei , Yu Pan , Yinghui Wang , Hui Cheng

Dental implant restoration has become one of the most important treatments for missing teeth. However, peri-implantitis is a common complication of implant restorations, and lack of timely and effective treatment will most likely lead to implant failure. Here, we developed a chitosan-based multifunctional temperature-sensitive hydrogel. Chitosan (CS)/β-glycerophosphate (β-GP) as the body of the hydrogel enabled a gelation transition at physiological temperatures. To enhance functionality, we designed Sim@ZIF8@PDA nanoparticles (SZP), where simvastatin (Sim) was loaded onto the zeolitic imidazolate framework-8 (ZIF8), leveraging its high drug-loading capacity and pH-sensitive release properties. These nanoparticles were further coated with polydopamine (PDA) to enhance photothermal efficiency and stability. The SZP nanoparticles were subsequently integrated with CS/β-GP to form the SZP/CS/β-GP hydrogel. The results showed that under near-infrared light irradiation, PDA exerted a photothermal effect to achieve anti-infection, while the release of simvastatin could promote the differentiation of CD4+ T cells to regulatory T cells (Tregs) to achieve immunoregulation. SZP/CS/β-GP hydrogel attenuated the infection and inflammation at the peri-implantitis lesion. In conclusion, this study provides a new approach for the non-surgical treatment of peri-implantitis.

{"title":"Nano Sim@ZIF8@PDA modified injectable temperature sensitive nanocomposite hydrogel for photothermal/drug therapy for peri-implantitis","authors":"Dingkun Liu , Jinbing Chen , Linjuan Gao , Xing Chen , Liujun Lin , Yuan Liu , Xia Wei , Yu Pan , Yinghui Wang , Hui Cheng","doi":"10.1016/j.carbpol.2025.123327","DOIUrl":"10.1016/j.carbpol.2025.123327","url":null,"abstract":"<div><div>Dental implant restoration has become one of the most important treatments for missing teeth. However, peri-implantitis is a common complication of implant restorations, and lack of timely and effective treatment will most likely lead to implant failure. Here, we developed a chitosan-based multifunctional temperature-sensitive hydrogel. Chitosan (CS)/β-glycerophosphate (β-GP) as the body of the hydrogel enabled a gelation transition at physiological temperatures. To enhance functionality, we designed Sim@ZIF8@PDA nanoparticles (SZP), where simvastatin (Sim) was loaded onto the zeolitic imidazolate framework-8 (ZIF8), leveraging its high drug-loading capacity and pH-sensitive release properties. These nanoparticles were further coated with polydopamine (PDA) to enhance photothermal efficiency and stability. The SZP nanoparticles were subsequently integrated with CS/β-GP to form the SZP/CS/β-GP hydrogel. The results showed that under near-infrared light irradiation, PDA exerted a photothermal effect to achieve anti-infection, while the release of simvastatin could promote the differentiation of CD4+ T cells to regulatory T cells (Tregs) to achieve immunoregulation. SZP/CS/β-GP hydrogel attenuated the infection and inflammation at the peri-implantitis lesion. In conclusion, this study provides a new approach for the non-surgical treatment of peri-implantitis.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"354 ","pages":"Article 123327"},"PeriodicalIF":10.7,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143357367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multifunctional dynamic chitosan-guar gum nanocomposite hydrogels in infection and diabetic wound healing

IF 10.7 1区化学 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymers

Pub Date : 2025-01-28 DOI: 10.1016/j.carbpol.2025.123316

Luning He , Shuyi Xing , Weikang Zhang , Ying Wang , Yian Li , Jinghao Chen , Jinghai Zhang , Siling Wang , Qinfu Zhao

Traditional wound care methods are less effective for infectious and diabetic wounds, highlighting an urgent need for effective strategies. The study aimed to design a self-healing hydrogel with antibacterial, antioxidant, and photothermal capabilities to treat infectious and diabetic wounds. Silver nanoparticles (AgNPs) were loaded into mesoporous polydopamine (MPDA) nanoparticles to form Ag@MPDA nanoparticles. Ag@MPDA was incorporated into the cationic guar gum-chitosan-boric acid (CCB) hydrogel to obtain the PA-CCB hydrogel. PA-CCB hydrogel exhibited excellent self-healing and adhesive properties, adapting well to the dynamic wound environment. PA-CCB hydrogel combined with photothermal therapy (PTT) could effectively eradicated E. coli (99.9 %) and S. aureus (99.7 %). The PA-CCB hydrogel reduced excessive reactive oxygen species and promoted the migration of fibroblasts in vitro. In the infected mouse wound models, the PA-CCB hydrogel effectively inhibited bacteria. After combining with PTT, the antibacterial ability of the PA-CCB hydrogel was further enhanced. In the diabetic mouse wound models, the PA-CCB hydrogel reduced the inflammatory level of wound tissue. In both models, after combining with PTT, the PA-CCB hydrogel exhibited further improvements in angiogenesis, collagen deposition, and re-epithelialization. By integrating multifunctional hydrogel with PTT, the PA-CCB hydrogel exhibited broad application potential for infectious and diabetic wounds.

{"title":"Multifunctional dynamic chitosan-guar gum nanocomposite hydrogels in infection and diabetic wound healing","authors":"Luning He , Shuyi Xing , Weikang Zhang , Ying Wang , Yian Li , Jinghao Chen , Jinghai Zhang , Siling Wang , Qinfu Zhao","doi":"10.1016/j.carbpol.2025.123316","DOIUrl":"10.1016/j.carbpol.2025.123316","url":null,"abstract":"<div><div>Traditional wound care methods are less effective for infectious and diabetic wounds, highlighting an urgent need for effective strategies. The study aimed to design a self-healing hydrogel with antibacterial, antioxidant, and photothermal capabilities to treat infectious and diabetic wounds. Silver nanoparticles (AgNPs) were loaded into mesoporous polydopamine (MPDA) nanoparticles to form Ag@MPDA nanoparticles. Ag@MPDA was incorporated into the cationic guar gum-chitosan-boric acid (CCB) hydrogel to obtain the PA-CCB hydrogel. PA-CCB hydrogel exhibited excellent self-healing and adhesive properties, adapting well to the dynamic wound environment. PA-CCB hydrogel combined with photothermal therapy (PTT) could effectively eradicated <em>E. coli</em> (99.9 %) and <em>S. aureus</em> (99.7 %). The PA-CCB hydrogel reduced excessive reactive oxygen species and promoted the migration of fibroblasts in vitro. In the infected mouse wound models, the PA-CCB hydrogel effectively inhibited bacteria. After combining with PTT, the antibacterial ability of the PA-CCB hydrogel was further enhanced. In the diabetic mouse wound models, the PA-CCB hydrogel reduced the inflammatory level of wound tissue. In both models, after combining with PTT, the PA-CCB hydrogel exhibited further improvements in angiogenesis, collagen deposition, and re-epithelialization. By integrating multifunctional hydrogel with PTT, the PA-CCB hydrogel exhibited broad application potential for infectious and diabetic wounds.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"354 ","pages":"Article 123316"},"PeriodicalIF":10.7,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143357405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chitosan surface interaction platform for protein binding quantification by fluorescence microscopy, application to the specificity of CBD proteins

IF 10.7 1区化学 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymers

Pub Date : 2025-01-28 DOI: 10.1016/j.carbpol.2025.123321

Lisa Basso , Guillaume Sudre , David Albertini , Yvan Rahbé , Laurent David

Surface-proteins interactions play key roles in many domains such as biomedicine, nanotechnology and the biology of plant-insect interactions. This article proposes a platform that allows to quantify surface interaction of proteins with chitin and chitosans to further discriminate and study specific interactions of proteins with chitin-binding domains (CBD). The platform consists in covalently grafted chitosan thin films of various degrees of acetylation (DA) through surface silanisation, spin-coating and water-temperature treatment. The obtained films were thoroughly characterized by infrared spectroscopy, contact angle measurements, atomic force microscopy and wide and small-angle X-ray scattering. Protein affinity to coated surfaces of reacetylated chitosans with degrees of acetylation ranging from 0.5 % to 76 % was evaluated by fluorescence microscopy. The specific affinity of lectins with a CBD was evidenced in comparison to proteins without CBD. As expected, the affinity was stronger at higher DAs, suggesting that the acetylation pattern play a part in specific lectin binding. In conclusion, chitosan films were fully characterized, and the elaborated platform shows promising results in screening protein interactions to chitin. This protein interaction platform is reportedly the first method able to differentiate the interactions of proteins containing a CBD and proteins which do not contain one, with whole chain “chitin-like” chitosans, by means of a simple and direct fluorescent microscopy quantification. This platform could further be used for other types of chitin-binding proteins or applied to other polysaccharide-protein interactions.

{"title":"Chitosan surface interaction platform for protein binding quantification by fluorescence microscopy, application to the specificity of CBD proteins","authors":"Lisa Basso , Guillaume Sudre , David Albertini , Yvan Rahbé , Laurent David","doi":"10.1016/j.carbpol.2025.123321","DOIUrl":"10.1016/j.carbpol.2025.123321","url":null,"abstract":"<div><div>Surface-proteins interactions play key roles in many domains such as biomedicine, nanotechnology and the biology of plant-insect interactions. This article proposes a platform that allows to quantify surface interaction of proteins with chitin and chitosans to further discriminate and study specific interactions of proteins with chitin-binding domains (CBD). The platform consists in covalently grafted chitosan thin films of various degrees of acetylation (DA) through surface silanisation, spin-coating and water-temperature treatment. The obtained films were thoroughly characterized by infrared spectroscopy, contact angle measurements, atomic force microscopy and wide and small-angle X-ray scattering. Protein affinity to coated surfaces of reacetylated chitosans with degrees of acetylation ranging from 0.5 % to 76 % was evaluated by fluorescence microscopy. The specific affinity of lectins with a CBD was evidenced in comparison to proteins without CBD. As expected, the affinity was stronger at higher DAs, suggesting that the acetylation pattern play a part in specific lectin binding. In conclusion, chitosan films were fully characterized, and the elaborated platform shows promising results in screening protein interactions to chitin. This protein interaction platform is reportedly the first method able to differentiate the interactions of proteins containing a CBD and proteins which do not contain one, with whole chain “chitin-like” chitosans, by means of a simple and direct fluorescent microscopy quantification. This platform could further be used for other types of chitin-binding proteins or applied to other polysaccharide-protein interactions.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"354 ","pages":"Article 123321"},"PeriodicalIF":10.7,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143323870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High internal phase Pickering emulsions stabilized by surface-modified dialdehyde xylan nanoparticles

IF 10.7 1区化学 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymers

Pub Date : 2025-01-27 DOI: 10.1016/j.carbpol.2025.123324

Huaiyu Zhang, Zemeng Wu, Jie Wu, Qi Hua, Yalan Liang, Scott Renneckar

Polysaccharide-based particles have attracted considerable attention for stabilizing Pickering emulsions due to their sustainability and biocompatibility. In this study, we developed a novel approach utilizing hemicellulose-based nanoparticles for the stabilization of high internal phase Pickering emulsions (HIPPEs). Polyethylenimine-modified dialdehyde xylan nanoparticles (PEI-DAXNPs) were prepared through periodate oxidation of xylan nanoparticles obtained from esparto pulp, followed by a Schiff base reaction with polyethylenimine (PEI). Oil-in-water HIPPEs were fabricated using PEI-DAXNPs as the sole stabilizer through a one-time homogenization method and exhibited long-term stability after 180 days of storage. Furthermore, gel-like HIPPEs were obtained with a minimum concentration of 0.1 wt% PEI-DAXNPs in the continuous phase and exhibited shear-thinning behavior and promising viscoelastic properties, indicating good processability in the fabrication of soft materials and porous scaffolds. Therefore, the produced PEI-DAXNPs demonstrated significant potential as HIPPE stabilizers, providing inspiration for the valorization of hemicellulose-based nanoparticles.

{"title":"High internal phase Pickering emulsions stabilized by surface-modified dialdehyde xylan nanoparticles","authors":"Huaiyu Zhang, Zemeng Wu, Jie Wu, Qi Hua, Yalan Liang, Scott Renneckar","doi":"10.1016/j.carbpol.2025.123324","DOIUrl":"10.1016/j.carbpol.2025.123324","url":null,"abstract":"<div><div>Polysaccharide-based particles have attracted considerable attention for stabilizing Pickering emulsions due to their sustainability and biocompatibility. In this study, we developed a novel approach utilizing hemicellulose-based nanoparticles for the stabilization of high internal phase Pickering emulsions (HIPPEs). Polyethylenimine-modified dialdehyde xylan nanoparticles (PEI-DAXNPs) were prepared through periodate oxidation of xylan nanoparticles obtained from esparto pulp, followed by a Schiff base reaction with polyethylenimine (PEI). Oil-in-water HIPPEs were fabricated using PEI-DAXNPs as the sole stabilizer through a one-time homogenization method and exhibited long-term stability after 180 days of storage. Furthermore, gel-like HIPPEs were obtained with a minimum concentration of 0.1 wt% PEI-DAXNPs in the continuous phase and exhibited shear-thinning behavior and promising viscoelastic properties, indicating good processability in the fabrication of soft materials and porous scaffolds. Therefore, the produced PEI-DAXNPs demonstrated significant potential as HIPPE stabilizers, providing inspiration for the valorization of hemicellulose-based nanoparticles.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"354 ","pages":"Article 123324"},"PeriodicalIF":10.7,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143357366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gamma rays irradiated polysaccharides: A review of the structure, physicochemical properties, biological activities alteration, and future food applications

IF 10.7 1区化学 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymers

Pub Date : 2025-01-27 DOI: 10.1016/j.carbpol.2025.123326

Yahya S. Hamed , Khloud R. Hassan , Mohamed E. Salem , Mingjie Shen , Jian Wang , Tingting Bu , Yan Cao , Qile Xia , Khaled M. Youssef , Kai Yang

Recently, there has been a growing interest in using radiation to change various properties of polysaccharides. This review gives a more detailed examination of the effects of gamma radiation on polysaccharides and its association with their techno-functional and biological properties following irradiation. Gamma irradiation is a potent tool for modifying the structure and properties of polysaccharides, enhancing their functionality in food applications. This review explores the effects of gamma irradiation on polysaccharides, focusing on changes in their molecular structure, physicochemical properties, and biological activities. Gamma irradiation induces chain scission and cross-linking in polysaccharides, leading to alterations in molecular weight, solubility, and viscosity. These structural modifications often enhance antioxidants, antimicrobial, and anti-inflammatory activities, expanding their potential use in food products. Gamma-irradiated polysaccharides exhibit improved gelation, emulsification, and film-forming abilities, making them suitable for various food applications such as thickeners, stabilizers, and edible coatings. The review also discusses the safety and regulatory aspects of using gamma-irradiated polysaccharides in food products. Future research directions are proposed to optimize irradiation conditions and further explore the multifunctional benefits of these modified polysaccharides, ultimately contributing to the development of innovative, functional food products.

{"title":"Gamma rays irradiated polysaccharides: A review of the structure, physicochemical properties, biological activities alteration, and future food applications","authors":"Yahya S. Hamed , Khloud R. Hassan , Mohamed E. Salem , Mingjie Shen , Jian Wang , Tingting Bu , Yan Cao , Qile Xia , Khaled M. Youssef , Kai Yang","doi":"10.1016/j.carbpol.2025.123326","DOIUrl":"10.1016/j.carbpol.2025.123326","url":null,"abstract":"<div><div>Recently, there has been a growing interest in using radiation to change various properties of polysaccharides. This review gives a more detailed examination of the effects of gamma radiation on polysaccharides and its association with their techno-functional and biological properties following irradiation. Gamma irradiation is a potent tool for modifying the structure and properties of polysaccharides, enhancing their functionality in food applications. This review explores the effects of gamma irradiation on polysaccharides, focusing on changes in their molecular structure, physicochemical properties, and biological activities. Gamma irradiation induces chain scission and cross-linking in polysaccharides, leading to alterations in molecular weight, solubility, and viscosity. These structural modifications often enhance antioxidants, antimicrobial, and anti-inflammatory activities, expanding their potential use in food products. Gamma-irradiated polysaccharides exhibit improved gelation, emulsification, and film-forming abilities, making them suitable for various food applications such as thickeners, stabilizers, and edible coatings. The review also discusses the safety and regulatory aspects of using gamma-irradiated polysaccharides in food products. Future research directions are proposed to optimize irradiation conditions and further explore the multifunctional benefits of these modified polysaccharides, ultimately contributing to the development of innovative, functional food products.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"354 ","pages":"Article 123326"},"PeriodicalIF":10.7,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143324033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Direct visualization of the side-by-side self-assembly of high and low acyl gellan gum by AFM

IF 10.7 1区化学 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymers

Pub Date : 2025-01-27 DOI: 10.1016/j.carbpol.2025.123325

Chun Peng, Fengxian Wang, Katsuyoshi Nishinari, Fatang Jiang, Man Xiao

This study explores the gelation mechanisms of high and low acyl gellan gum (HAGG and LAGG) by analyzing their chain conformations and self-assembly behaviour during cooling. Using statistical analysis of atomic force microscopy (AFM) images, differential scanning calorimetry (DSC), and rheological measurements, we examined the cooling process from 90 °C to 25 °C for HAGG and from 90 °C to 10 °C for LAGG. Both types of gellan gum transitioned from single-stranded chains to double-helical structures. HAGG exhibited semi-flexible single and double-helical chains with shorter persistence lengths, prominent kinks, and larger kink angles, forming double helices through intra- and interchain interactions. These helices then underwent side-by-side self-assembly into multi-branched, elastic networks. Conversely, LAGG displayed more rigid chains with longer persistence lengths, fewer kinks, and smaller kink angles, forming double helices via interchain interactions, followed by side-by-side self-assembly into less-branched, rigid networks. AFM directly visualized these structural transitions, notably the side-by-side self-assembly for the first time, supported by DSC and rheological data. The results provide new evidence on gellan gum's gelation mechanisms and self-assembly behaviour in pure water.

{"title":"Direct visualization of the side-by-side self-assembly of high and low acyl gellan gum by AFM","authors":"Chun Peng, Fengxian Wang, Katsuyoshi Nishinari, Fatang Jiang, Man Xiao","doi":"10.1016/j.carbpol.2025.123325","DOIUrl":"10.1016/j.carbpol.2025.123325","url":null,"abstract":"<div><div>This study explores the gelation mechanisms of high and low acyl gellan gum (HAGG and LAGG) by analyzing their chain conformations and self-assembly behaviour during cooling. Using statistical analysis of atomic force microscopy (AFM) images, differential scanning calorimetry (DSC), and rheological measurements, we examined the cooling process from 90 °C to 25 °C for HAGG and from 90 °C to 10 °C for LAGG. Both types of gellan gum transitioned from single-stranded chains to double-helical structures. HAGG exhibited semi-flexible single and double-helical chains with shorter persistence lengths, prominent kinks, and larger kink angles, forming double helices through intra- and interchain interactions. These helices then underwent side-by-side self-assembly into multi-branched, elastic networks. Conversely, LAGG displayed more rigid chains with longer persistence lengths, fewer kinks, and smaller kink angles, forming double helices via interchain interactions, followed by side-by-side self-assembly into less-branched, rigid networks. AFM directly visualized these structural transitions, notably the side-by-side self-assembly for the first time, supported by DSC and rheological data. The results provide new evidence on gellan gum's gelation mechanisms and self-assembly behaviour in pure water.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"354 ","pages":"Article 123325"},"PeriodicalIF":10.7,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143357369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

pH-responsive and self-adaptive injectable sodium alginate/carboxymethyl chitosan hydrogel accelerates infected wound healing by bacteriostasis and immunomodulation

IF 10.7 1区化学 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymers

Pub Date : 2025-01-27 DOI: 10.1016/j.carbpol.2025.123322

Xi Zhang, Yongzhe Liu, Ziwei Wang, Hongxia Zhao, Longxuan Zhan, Houda Gui, Xin Xu, Xiaoni Ma, Baojin Ma

Infected wound healing is a global medical challenge due to persistent bacterial infection and excess inflammation. Designing microenvironment-responsive and self-adaptive hydrogels with antibacterial and anti-inflammatory properties is expected to be an effective strategy to promote infected wound healing. In this study, a self-adaptive injectable sodium alginate/carboxymethyl chitosan hydrogel (SCSC) for pH-responsive release of curcumin has been prepared at room temperature by simple stirring. The incorporation of the crosslinker, Sr²⁺, enhances mechanical properties of SCSC hydrogel by tensing network structures, and provides synergistic biological activities. Meanwhile, SCSC hydrogel self-adapts in irregular wound shapes with outstanding ECM-like performance and releases effectively in the acidic microenvironment simulating the initial stage of infected wounds. SCSC hydrogel with good biocompatibility can promote cell migration, while relieving oxidative stress and mitochondria damage. Notably, SCSC hydrogel inhibits inflammatory factor expression and promotes M2 macrophage polarization by suppressing the NF-κB signaling pathway. Further, SCSC hydrogel significantly downregulates the over-expression of matrix metalloproteinase-9 (MMP-9), thereby helping promote ECM reconstruction. In vivo experiment results further demonstrate that SCSC hydrogel accelerates the re-epithelialization of infected wounds on the back of rats. Thus, the pH-responsive and self-adaptive SCSC hydrogel possesses great potential in the management and control of infected wounds.

{"title":"pH-responsive and self-adaptive injectable sodium alginate/carboxymethyl chitosan hydrogel accelerates infected wound healing by bacteriostasis and immunomodulation","authors":"Xi Zhang, Yongzhe Liu, Ziwei Wang, Hongxia Zhao, Longxuan Zhan, Houda Gui, Xin Xu, Xiaoni Ma, Baojin Ma","doi":"10.1016/j.carbpol.2025.123322","DOIUrl":"10.1016/j.carbpol.2025.123322","url":null,"abstract":"<div><div>Infected wound healing is a global medical challenge due to persistent bacterial infection and excess inflammation. Designing microenvironment-responsive and self-adaptive hydrogels with antibacterial and anti-inflammatory properties is expected to be an effective strategy to promote infected wound healing. In this study, a self-adaptive injectable sodium alginate/carboxymethyl chitosan hydrogel (SCSC) for pH-responsive release of curcumin has been prepared at room temperature by simple stirring. The incorporation of the crosslinker, Sr<sup>2+</sup>, enhances mechanical properties of SCSC hydrogel by tensing network structures, and provides synergistic biological activities. Meanwhile, SCSC hydrogel self-adapts in irregular wound shapes with outstanding ECM-like performance and releases effectively in the acidic microenvironment simulating the initial stage of infected wounds. SCSC hydrogel with good biocompatibility can promote cell migration, while relieving oxidative stress and mitochondria damage. Notably, SCSC hydrogel inhibits inflammatory factor expression and promotes M2 macrophage polarization by suppressing the NF-κB signaling pathway. Further, SCSC hydrogel significantly downregulates the over-expression of matrix metalloproteinase-9 (MMP-9), thereby helping promote ECM reconstruction. <em>In vivo</em> experiment results further demonstrate that SCSC hydrogel accelerates the re-epithelialization of infected wounds on the back of rats. Thus, the pH-responsive and self-adaptive SCSC hydrogel possesses great potential in the management and control of infected wounds.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"354 ","pages":"Article 123322"},"PeriodicalIF":10.7,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143324036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel injectable sodium alginate/chitosan/sulfated bacterial cellulose hydrogel as biohybrid artificial pancreas for real-time glycaemic regulation

IF 10.7 1区化学 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymers

Pub Date : 2025-01-27 DOI: 10.1016/j.carbpol.2025.123323

Xiang Zhao , Wei Xue , Weixiao Ding , Yalei Qiao , Xuehui Chu , Yudong Qiu , Min Tang , Dongping Sun , Xiao Fu

Type 1 diabetes mellitus (T1DM) are characterized by blood glucose elevation with pancreatic β cells deficiency. As a safe alternative to frequent subcutaneous insulin injection, pancreatic β cell transplantation provides a promising therapeutic option for blood glucose control in T1DM. However, pancreatic β cell transplantation faces intractable challenges of the poor viability and severe host immune rejection. Therefore, a novel approach capable of improving the poor oxygen/nutrients supply and severe host immune rejection is highly desired. Herein, a novel biohybrid artificial pancreas, presenting glucose-dependent insulin release behavior, is constructed via pancreatic β cells encapsulating in a hydrogel scaffold. The hydrogel scaffold is made of the commixture of sodium alginate (SA), chitosan (CS) and sulfated bacterial cellulose (SBC). The biocompatible three-dimensional (3D) hydrogels protected pancreatic β cells from immune response but also allowed the exchange of nutrients and insulin. As a result of the synergistic effect, the biohybrid artificial pancreas can reverse the hyperglycemia and achieve sustained glycemic control for at least 30 days in diabetic mice. Collectively, we consider that this biohybrid artificial pancreas with an elaborate structure could provide an effective option for the treatment of type 1 diabetes.

{"title":"A novel injectable sodium alginate/chitosan/sulfated bacterial cellulose hydrogel as biohybrid artificial pancreas for real-time glycaemic regulation","authors":"Xiang Zhao , Wei Xue , Weixiao Ding , Yalei Qiao , Xuehui Chu , Yudong Qiu , Min Tang , Dongping Sun , Xiao Fu","doi":"10.1016/j.carbpol.2025.123323","DOIUrl":"10.1016/j.carbpol.2025.123323","url":null,"abstract":"<div><div>Type 1 diabetes mellitus (T1DM) are characterized by blood glucose elevation with pancreatic β cells deficiency. As a safe alternative to frequent subcutaneous insulin injection, pancreatic β cell transplantation provides a promising therapeutic option for blood glucose control in T1DM. However, pancreatic β cell transplantation faces intractable challenges of the poor viability and severe host immune rejection. Therefore, a novel approach capable of improving the poor oxygen/nutrients supply and severe host immune rejection is highly desired. Herein, a novel biohybrid artificial pancreas, presenting glucose-dependent insulin release behavior, is constructed via pancreatic β cells encapsulating in a hydrogel scaffold. The hydrogel scaffold is made of the commixture of sodium alginate (SA), chitosan (CS) and sulfated bacterial cellulose (SBC). The biocompatible three-dimensional (3D) hydrogels protected pancreatic β cells from immune response but also allowed the exchange of nutrients and insulin. As a result of the synergistic effect, the biohybrid artificial pancreas can reverse the hyperglycemia and achieve sustained glycemic control for at least 30 days in diabetic mice. Collectively, we consider that this biohybrid artificial pancreas with an elaborate structure could provide an effective option for the treatment of type 1 diabetes.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"354 ","pages":"Article 123323"},"PeriodicalIF":10.7,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143357368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Different molecular weight fucogalactans from Macrocybe titans mushroom promote distinct effect on breast cancer cell death

IF 10.7 1区化学 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymers

Pub Date : 2025-01-25 DOI: 10.1016/j.carbpol.2025.123318

Shayane da Silva Milhorini , Renata Rutckeviski , Ariana Centa , Fhernanda Ribeiro Smiderle , Matheus Zavadinack , Fábio Rogério Rosado , Marcello Iacomini

There is an incessant search for new therapies against cancer, able to unite effectiveness with specificity, leading to higher survival rates and lower deleterious effects. Herein, two fucogalactans (F-1 and F-2), isolated from Macrocybe titans, showed a (1 → 6)-linked α-D-Galp main chain partially substituted at O-2 by non-reducing end units of α-L-Fucp, with different M_w, being F-2 > 20 times higher than F-1. Both fucogalactans induced cell cycle arrest of MDA-MB-231 cells in G1 phase after 120 h of treatment. However, only F2 resulted in increased apoptosis and necrosis. For the MCF-7 cell line, no changes in the cell cycle and cell death were observed at 120 h. The non-tumoral cell line (VERO) was not affected. The results confirmed that polysaccharides with different M_w may have distinct effects and therefore this is an important feature to be considered on investigating anti-cancer treatments.

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引用次数: 0

Structural characterization and chondroprotective activity evaluation of four novel polysaccharides purified from Anoectochilus zhejiangensis on transgenic fluorescent zebrafish

IF 10.7 1区化学 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymers

Pub Date : 2025-01-25 DOI: 10.1016/j.carbpol.2025.123319

Yi Tao , Lisha Shen , Wei Luo , Ping Wang

Anoectochilus zhejiangensis (AZJ) exhibits notable anti-inflammatory and anti-swelling properties, making it a potential therapeutic agent for osteoarthritis. However, the specific component responsible for its anti-osteoarthritis effects remains unidentified. In this study, four novel polysaccharides were purified from Anoectochilus zhejiangensis (i.e., AZJP-1a, AZJP-2a, AZJP-2b, and AZJP-2c) through DEAE-cellulose 52 and Sephadex G-200 column chromatographic separation. Their structural and conformational characteristics were comprehensively analyzed. AZJP-1a and AZJP-2a owned high molecular weights of 387 kDa and 947 kDa, while AZJP-2b and AZJP-2c were comparatively lower at 3.989 kDa and 3.045 kDa. The polysaccharides contained predominantly β-glycosidic linkages over α-glycosidic linkages. AZJP-1a primarily consists of mannose, while AZJP-2a and AZJP-2b are rich in glucose, galactose, and arabinose, and AZJP-2c is mainly composed of glucose. Chondroprotective effects of these polysaccharides were evaluated using fluorescence imaging in transgenic fluorescent zebrafish (Tg Col2a1a: eGFP), with all four polysaccharides demonstrating significant cartilage repair activity, surpassing that of the positive control drug alendronate. Among them, AZJP-2c exhibited the most potent effect. The observed variations in their biological activities are likely attributed to differences in their structural compositions.

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引用次数: 0

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