Carbohydrate Polymers最新文献_第7页

Engineering compartmentalized utilization of glycerol with glucose for high-level chondroitin production in Corynebacterium glutamicum 工程分区利用甘油和葡萄糖在谷氨酸棒状杆菌生产高水平的软骨素

IF 12.5 1区化学 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymers

Pub Date : 2026-01-16 DOI: 10.1016/j.carbpol.2026.124946

Zhuangzhuang Shi , Yue Ming , Jue Li , Yiting Lin , Weilong Wang , Wei Sha , Guoqiang Li , Mengmeng Wu , Ting Ma

Chondroitin sulfate (CS) is an important glycosaminoglycan widely used in biomedical and nutraceutical fields. However, its commercial production remains dependent on extraction from animal tissues. Microbial synthesis of its unsulfated precursor, chondroitin, followed by enzymatic sulfation, presents a sustainable alternative. Nevertheless, low chondroitin titer remains a major bottleneck for efficient biosynthesis. This study established an efficient Corynebacterium glutamicum cell factory for enhanced chondroitin production. The base chassis strain CgC25 was engineered by deleting lactic and acetic acid synthesis pathways and blocking glycolysis. To compensate for metabolic impairments, an optimized glycerol utilization pathway was implemented via an RBS library, establishing a parallel carbon metabolism system. Redox homeostasis was also reinforced to alleviate metabolic stress. The final strain CgC40 achieved a titer of 12.37 ± 0.51 g/L with a yield of 102.94 ± 4.90 mg/g carbon source, increased by 24.20% and 30.07%, respectively. This titer constitutes the highest level reported to date. Multi-omics analysis further confirmed the enhanced chondroitin synthesis and a corresponding reduction in TCA cycle activity. This work demonstrates an effective metabolic reprogramming strategy that coordinated carbon co-utilization to enhance bioproduction efficiency, providing a broadly applicable platform for synthesizing diverse high-value compounds.

硫酸软骨素（CS）是一种重要的糖胺聚糖，广泛应用于生物医学和营养保健领域。然而，它的商业生产仍然依赖于从动物组织中提取。微生物合成其无硫酸盐前体，软骨素，其次是酶的硫酸，提出了一个可持续的选择。然而，低软骨素滴度仍然是有效生物合成的主要瓶颈。本研究建立了一个高效的谷氨酸棒状杆菌细胞工厂，以提高软骨素的生产。通过删除乳酸和乙酸合成途径，阻断糖酵解，构建了基底盘应变CgC25。为了弥补代谢损伤，通过RBS库实现了优化的甘油利用途径，建立了一个平行的碳代谢系统。氧化还原稳态也被加强，以减轻代谢应激。最终菌株CgC40的滴度为12.37±0.51 g/L，碳源产率为102.94±4.90 mg/g，分别提高了24.20%和30.07%。该滴度是迄今报告的最高水平。多组学分析进一步证实了增强的软骨素合成和相应的TCA循环活性降低。这项工作展示了一种有效的代谢重编程策略，通过协调碳共利用来提高生物生产效率，为合成各种高价值化合物提供了广泛适用的平台。

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引用次数: 0

Wine processing induced structural modification of Polygonati Rhizoma polysaccharide and their enhancement of Tc1 cell immunomodulatory activity via microbiota-mediated butyrate metabolism 葡萄酒加工诱导黄精多糖结构改变，并通过微生物介导的丁酸盐代谢增强Tc1细胞免疫调节活性

IF 12.5 1区化学 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymers

Pub Date : 2026-01-16 DOI: 10.1016/j.carbpol.2026.124935

Fenghe Yang , Chun Wang , Zhiqian Song , Shitao Peng , Ziqi Tian , Wenjie Lu , Tao Lin , Qiao He , Aiping Lyu , Zhangchi Ning

The herb Polygonati Rhizoma is highly esteemed for its immune regulatory therapeutic and nutritive properties, with polysaccharide identified as the principal components that play a pivotal role in immune response modifying. In China, the immunomodulatory effects of Polygonati Rhizoma polysaccharide (PRP) are believed to enhanced after wine processing. However, the structural changes and underlying mechanisms remain insufficiently elucidated. We isolated and purified PRP and wine processing PRP (WPRP) for structural and functional analysis. Structural analysis showed a processing-driven shift from a low-molecular-weight fructo-glucosyl fructan in PRP (1.85 kDa) to a higher-molecular-weight, pectin-type polysaccharide in WPRP (7.31 kDa) enriched in arabinose, galactose and galacturonic acid. The precise structural transition was from α-D-Glcp → [2)-β-D-Fruf-(1]₃ → 2)-β-D-Fruf-(1 → 6)-α-D-Glcp-(1 → 2)-β-D-Fruf → in PRP to → 4)-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 4)-α-D-GalAp-(1 → 5)-α-L-Araf-(1 → [5)-α-L-Araf-(1]₃ → 4)-α-D-GalAp-(1 → 4)-α-D-GalAp-(1 → in WPRP. WPRP exhibited stronger immunomodulatory activity than PRP in immunosuppressed mice. Mechanistically, galacturonic acid-rich pectins and arabinan driven by wine processing are preferential fermentable substrates for butyrate-producing gut bacteria, which promoted Tc1 cell activation/differentiation, and preserved intestinal epithelial integrity. These findings reveal that PRP drives structural remodeling after wine processing, modulating microbial butyrate metabolism and promoting Tc1 activation, which provide novel insights into the modern medical value of traditional dietary resources.

中药黄精因其免疫调节治疗和营养特性而备受推崇，多糖被确定为在免疫反应调节中起关键作用的主要成分。在中国，黄精多糖（PRP）在葡萄酒加工后的免疫调节作用被认为是增强的。然而，结构变化和潜在的机制仍然没有充分阐明。我们分离纯化了PRP和葡萄酒加工PRP （WPRP）进行了结构和功能分析。结构分析表明，在加工过程中，PRP从低分子量的果糖-葡萄糖基果聚糖（1.85 kDa）转变为高分子量的果胶型多糖（7.31 kDa），富含阿拉伯糖、半乳糖和半乳糖醛酸。精确的结构转变是α-D-Glcp→(2),β-D-Fruf——(1)₃→2)-β-D-Fruf -(1→6)α-D-Glcp -(1→2)β-D-Fruf→PRP→4)-β-D-Galp -(1→4)-β-D-Galp -(1→4)-α-D-GalAp -(1→5)α-L-Araf -(1→[5)α-L-Araf——(1)₃→4)-α-D-GalAp -(1→4)-α-D-GalAp -(1→WPRP。WPRP在免疫抑制小鼠中表现出比PRP更强的免疫调节活性。从机制上讲，富含半乳糖醛酸的果胶和由葡萄酒加工驱动的阿拉伯糖是产生丁酸的肠道细菌的首选发酵底物，可以促进Tc1细胞的活化/分化，并保持肠道上皮的完整性。这些发现揭示了PRP驱动葡萄酒加工后的结构重塑，调节微生物丁酸盐代谢，促进Tc1活化，为传统膳食资源的现代医学价值提供了新的见解。

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引用次数: 0

Micro-nano structured bioplastics from natural biomass enabled by chitin nanofiber binders 由天然生物质制成的微纳结构生物塑料由几丁质纳米纤维粘结剂制成

IF 12.5 1区化学 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymers

Pub Date : 2026-01-16 DOI: 10.1016/j.carbpol.2026.124947

Yiran Zheng , Yuhui Liang , Yuxin Feng , Ke Jiang , Bo Duan

Renewable materials derived from biomass are promising alternatives to non-biodegradable petrochemical plastics. Herein, we present a method for producing high-performance bioplastics by using the regenerated chitin nanofibers (ChNFs) as the binder material for micro-scale biomass materials (bamboo microfibers or chitin microsheets). The multi-scale biomass fibers were processed into the slurry and subsequently dried to induce self-assembly. ChNFs adhere to micro-sized materials and form uniform nanoscale entanglements within their pores, enhancing the interfacial interactions among microscale biomass materials, which enable the bamboo-based bioplastic to achieve a tensile strength of 73.74 ± 4.04 MPa. The ChNFs also improve the water stability, maintaining stability in water of 8 h, and enhance hydroplasticity for shaping. Additionally, ChNFs act as an adhesive in a weakly acidic environment, tightly bonding films together through the dissolution and regeneration. The bioplastics are made from natural polysaccharide-based polymers, which can be easily recycled and biodegradable. By combining micro- and nano-biomass materials, this strategy offers a promising route for producing strong, biodegradable, and sustainable biomass plastics as viable alternatives to petrochemical plastics.

从生物质中提取的可再生材料是不可生物降解的石化塑料的有希望的替代品。本文提出了一种利用再生几丁质纳米纤维（ChNFs）作为微尺度生物质材料（竹微纤维或几丁质微片）的粘结材料来制备高性能生物塑料的方法。将多尺度生物质纤维加工成浆料并随后干燥以诱导自组装。chnf粘附在微尺度材料上，并在其孔隙内形成均匀的纳米级缠结，增强了微尺度生物质材料之间的界面相互作用，使竹基生物塑料的抗拉强度达到73.74±4.04 MPa。chnf还提高了水稳定性，在水中保持8 h的稳定性，并增强了成型的水塑性。此外，ChNFs在弱酸性环境中充当粘合剂，通过溶解和再生将膜紧密地粘合在一起。生物塑料是由天然的多糖类聚合物制成的，可以很容易地回收和生物降解。通过结合微纳米生物质材料，这一策略为生产强大的、可生物降解的、可持续的生物质塑料提供了一条有前途的途径，作为石化塑料的可行替代品。

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引用次数: 0

Tough and stretchable cellulose hydrogels engineered via the synergy of entanglements and cross-links 坚韧和可拉伸的纤维素水凝胶工程通过缠绕和交联的协同作用

IF 12.5 1区化学 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymers

Pub Date : 2026-01-16 DOI: 10.1016/j.carbpol.2026.124915

Pingdong Wei , Lei Wang , Hao Zhang , Xinyu Chen , Caizhen Zhu , Jie Cai

Hydrogels are attractive for various applications, including engineering artificial tissue, flexible electronic devices, and structural biomaterials, due to their advantageous characteristics such as flexibility, hydrophilicity, and biocompatibility. However, the strengthening and toughening of sustainable crystalline polysaccharide hydrogels remain challenging due to their high water content and limited energy dissipation mechanisms. Here we present a strategy to produce a dual cross-linked cellulose hydrogel with remarkable toughness and stretchability via the synergy of entanglements and cross-links in a hierarchical structure. The cellulose hydrogels are consisted of cellulose chains that strongly interact with each other through physical interactions, while both cellulose chains and long-chain chemical cross-linkers are densely entangled in molecular-scale, which lead to an intertwined nanofibrillar architecture with high content of cellulose II crystalline hydrates in nano- and micro-scale. The resultant macroscale cellulose hydrogels have a water content ranging from 72% to 82%. The maximum values for tensile strength, tensile strain, and work of fracture were 9.5 ± 2 MPa, 267 ± 18%, and 11.7 ± 0.3 MJ/m³, respectively. The strategy suggested in this study has the potential to be extended to other biomacromolecules, thereby enhancing the applicability of structural hydrogels in scenarios that demand superior mechanical properties.

由于其柔韧性、亲水性和生物相容性等优点，水凝胶在工程人工组织、柔性电子器件和结构生物材料等领域具有广泛的应用前景。然而，由于其高含水量和有限的能量耗散机制，可持续结晶多糖水凝胶的强化和增韧仍然具有挑战性。在这里，我们提出了一种策略，以生产双交联纤维素水凝胶具有显著的韧性和拉伸性，通过在一个层次结构的纠缠和交联的协同作用。纤维素水凝胶由纤维素链组成，它们之间通过物理相互作用产生强烈的相互作用，而纤维素链和长链化学交联剂在分子尺度上紧密纠缠，在纳米和微观尺度上形成了纤维素II型结晶水合物含量高的缠绕纳米纤维结构。所得大尺度纤维素水凝胶的含水量为72% ~ 82%。拉伸强度、拉伸应变和断裂功的最大值分别为9.5±2 MPa、267±18%和11.7±0.3 MJ/m3。本研究提出的策略有可能扩展到其他生物大分子，从而增强结构水凝胶在需要优越机械性能的场景中的适用性。

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引用次数: 0

Corrigendum to “Starch nanoparticle platform for oral delivery of sinigrin in colitis therapy” [Carbohydrate Polymers 367 (2025) 124032] “在结肠炎治疗中口服紫sinigin的淀粉纳米颗粒平台”的更正[碳水化合物聚合物367 (2025)124032]

IF 12.5 1区化学 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymers

Pub Date : 2026-01-15 DOI: 10.1016/j.carbpol.2026.124912

Hazzel Joy Adra , Hanvit Cha , Moon Han Chang , Dong-Gook Kang , Su-min Kwon , Sang-Mook You , Yu Ri Jeong , Chae Hwan Lee , Ki Sung Park , Seung Pil Pack , Jin Hyup Lee , Young-Rok Kim

引用次数: 0

Orange peel-derived pectin/cellulose nanofibril hydrogel reinforced by Eu-MOF for simultaneous adsorption and detection of levofloxacin Eu-MOF增强橙皮果胶/纤维素纳米纤维水凝胶同时吸附和检测左氧氟沙星

IF 12.5 1区化学 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymers

Pub Date : 2026-01-15 DOI: 10.1016/j.carbpol.2026.124943

Yuting Zhang , Xinmiao Qi , Binzhen Liu , Liangkun Li , Xin Guo

Orange peel is an abundant polysaccharide-rich agro-industrial residue that is often discarded, causing resource loss and environmental burden. Here, we valorize orange peel-derived pectin into a mechanically robust and photostable fluorescent hydrogel (EuCPH) by embedding uniformly dispersed Europium-based metal-organic framework decorated cellulose nanofibril (Eu-MOF@CNF). The resulting red-emissive EuCPH enables simultaneous levofloxacin (LEV) capture and fluorescence sensing. EuCPH-4 exhibits an ultrahigh Langmuir capacity of 1388.6 mg g⁻¹ and a low limit of detection (LOD) of 0.12 μg L⁻¹. Adsorption follows the Langmuir isotherm and pseudo-second-order kinetics, consistent with chemisorption-dominated uptake. EuCPH-4 retains 83.8% of its capacity after five cycles with negligible Eu leaching (<8 ppb) and sustains stable performance over ten fixed-bed regeneration cycles. With low-cost orange peel-derived pectin and wood-derived CNF, EuCPH can be produced at ~0.02 CNY g⁻¹, providing a scalable and economically viable hydrogel platform for antibiotic monitoring and remediation.

桔皮是一种丰富的富含多糖的农工废渣，经常被丢弃，造成资源损失和环境负担。在这里，我们通过嵌入均匀分散的铕基金属有机框架修饰的纤维素纳米纤维（Eu-MOF@CNF），将橙子皮衍生的果胶转化为机械坚固且光稳定的荧光水凝胶（EuCPH）。由此产生的红发射EuCPH能够同时捕获左氧氟沙星（LEV）和荧光传感。EuCPH-4的Langmuir容量为1388.6 mg g−1，低检出限为0.12 μg L−1。吸附遵循Langmuir等温线和准二级动力学，与化学吸附为主的吸收一致。euph -4在5个循环后仍能保持83.8%的容量，且在10个固定床再生循环中保持稳定的性能。利用低成本的橘子皮衍生果胶和木材衍生CNF， EuCPH的生产成本为~0.02 CNY g−1，为抗生素监测和修复提供了一个可扩展且经济可行的水凝胶平台。

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引用次数: 0

Multifunctional conductive hydrogel based on carboxymethyl cellulose/oxidized sodium alginate for machine learning-guided sports training 基于羧甲基纤维素/氧化海藻酸钠的多功能导电水凝胶用于机器学习引导的运动训练

IF 12.5 1区化学 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymers

Pub Date : 2026-01-14 DOI: 10.1016/j.carbpol.2026.124942

Zhenchun Li , Rongfeng Ge , Ning Li , Jiaqi Hou , Zhiyuan Zhao , Jieli Hua , Chuang Du , Lei Wang

Multifunctional hydrogels have shown great promise in advanced wearable sensing technologies; however, their performance under real-world sweaty and dynamic conditions remains a significant challenge. In this study, a highly conductive and adhesive hydrogel was developed using poly(AM-co-AA), carboxymethyl cellulose (CMC), oxidized sodium alginate (OSA), and polydopamine-modified carbon nanotubes (PDA-CNT). Featuring a dual dynamic cross-linking system based on reversible Schiff base bonds and strong hydrogen bonding, the hydrogel demonstrates high mechanical toughness (2416 kJ/m³), excellent stretchability (1829%), and stable electrical conductivity (1.5 S/m). It exhibits outstanding sweat resistance, maintaining strong adhesion and reliable sensing performance even during intense physical activities, along with high fatigue resistance over 10,000 deformation cycles, enabling long-term reliable monitoring. As a highly sensitive strain sensor, it offers multi-range gauge factors (up to 2.53) and accurately captures human motion and physiological signals without interference from sweat or motion artifacts. When integrated with machine learning algorithms, the sensor achieves a validation accuracy of 98.75%, providing real-time feedback on movement techniques and supporting personalized training optimization. Furthermore, the hydrogel demonstrates significant antibacterial properties and good biocompatibility, making it an ideal candidate for next-generation wearable electronic devices suited for long-term athletic training and high-sweat environments.

多功能水凝胶在先进的可穿戴传感技术中显示出巨大的前景；然而，他们的性能在真实的汗水和动态条件下仍然是一个重大的挑战。本研究以聚（AM-co-AA）、羧甲基纤维素（CMC）、氧化海藻酸钠（OSA）和聚多巴胺修饰的碳纳米管（PDA-CNT）为原料，制备了一种高导电性和粘性的水凝胶。该水凝胶具有基于可逆希夫碱键和强氢键的双动态交联体系，具有高机械韧性（2416 kJ/m3）、优异的拉伸性（1829%）和稳定的电导率（1.5 S/m）。它具有出色的抗汗性，即使在激烈的身体活动中也能保持强大的附着力和可靠的传感性能，同时具有超过10,000次变形周期的高抗疲劳性，可实现长期可靠的监测。作为一种高灵敏度的应变传感器，它提供多量程测量因子（高达2.53），并准确捕获人体运动和生理信号，而不会受到汗水或运动伪影的干扰。当与机器学习算法集成时，传感器的验证精度达到98.75%，提供运动技术的实时反馈，并支持个性化训练优化。此外，水凝胶具有显著的抗菌性能和良好的生物相容性，使其成为适合长期运动训练和高汗环境的下一代可穿戴电子设备的理想候选者。

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引用次数: 0

Light-driven switchable polyacrylamide/chitosan-based hydrogel dressings for outdoor wound temperature regulation and enhanced skin regeneration 用于室外伤口温度调节和增强皮肤再生的光驱动可切换聚丙烯酰胺/壳聚糖基水凝胶敷料

IF 12.5 1区化学 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymers

Pub Date : 2026-01-14 DOI: 10.1016/j.carbpol.2026.124920

Tianyi Lu , Qianqian Wang , Meng Sun , Shuangjiang Feng , Renhao Ding , Haoyu Xiao , Shanhu Qiu , Zhonghua Ni , Xiao Li , Tao Hu

Proper temperature management has been acknowledged for wound repair, but skin wounds exposed to the outdoors are inevitably susceptible to intense solar radiation and extreme temperatures. However, the wound thermal environment is easily disrupted even treated by the functional dressings with limited thermal adaptability, gradually hindering the process of wound healing. Herein, we propose an outdoor light-driven dual thermal management system sequentially employing cooling and heating dressings to optimize the temperature of wounds and accelerate wound healing. The cooling dressing integrates a light porous poly(vinyl alcohol)/SiO₂ aerogel exhibiting high solar reflectance (≈91.5%) and strong mid-infrared emissivity (≈95.6%) with a self-adhesive, antibacterial polyacrylamide/chitosan hydrogel. It achieves substantial coupled radiative and evaporative cooling in the outdoors, lowering the rat wound temperature by approximately 6 °C under direct sunlight. Conversely, the heating dressing of polyacrylamide/chitosan/CNT-COOH hydrogel can rapidly reach ≈45 °C under near-infrared irradiation (808 nm, 0.5 W cm⁻²), providing mild photothermal antibacterial effects. Critically, the sequential application of cooling and heating dressings in a wound model of SD rats notably enhanced skin regeneration. This work develops a low-cost, energy-efficient approach for outdoor wound thermal environment regulation, inspiring the design of personalized healthcare devices under detrimental environmental conditions.

适当的温度管理已被公认为伤口修复，但皮肤伤口暴露在户外不可避免地容易受到强烈的太阳辐射和极端温度。然而，即使使用热适应性有限的功能性敷料处理，创面热环境也容易被破坏，逐渐阻碍创面愈合的进程。在此，我们提出了一种室外光驱动双热管理系统，采用冷却和加热敷料来优化伤口温度，加速伤口愈合。该冷却包扎将具有高太阳反射率（≈91.5%）和强中红外发射率（≈95.6%）的轻质多孔聚乙烯醇/SiO2气凝胶与自粘、抗菌的聚丙烯酰胺/壳聚糖水凝胶相结合。它在室外实现了大量的耦合辐射和蒸发冷却，在阳光直射下将大鼠伤口温度降低了约6°C。相反，聚丙烯酰胺/壳聚糖/CNT-COOH水凝胶在近红外辐射（808 nm, 0.5 W cm−2）下的加热包扎可迅速达到≈45℃，具有温和的光热抗菌效果。重要的是，在SD大鼠伤口模型中连续应用冷却和加热敷料可显著促进皮肤再生。这项工作为室外伤口热环境调节开发了一种低成本、节能的方法，启发了在有害环境条件下个性化医疗设备的设计。

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引用次数: 0

pH/pectinase dual-responsive pectin-chitosan nanoparticles for pathogen-activated delivery of α-Terthienyl against Botrytis cinerea. pH/果胶酶双响应的果胶-壳聚糖纳米颗粒用于病原体激活的α-三硫乙烯基对葡萄孢菌的递送。

IF 12.5 1区化学 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymers

Pub Date : 2026-01-14 DOI: 10.1016/j.carbpol.2026.124938

Junhao Ye , Ruiquan Hou , Kang Xie , Guangmin Ou , Zhixiang Zhang , Dongmei Cheng

The plant-derived photoactivated fungicide α-Terthienyl (α-T) exerts antimicrobial effects through light-induced reactive oxygen species generation. However, its agricultural application has been constrained by poor targeting capability and low photostability. To address these challenges, we developed a dual-responsive nanoparticle delivery system (α-T NPs) based on a pectin-chitosan polymer network that specifically recognizes the acidic and pectinase-rich microenvironment created by Botrytis cinerea hyphae secretions. Controlled release studies demonstrated that both acidic conditions (pH 5.0) and pectinase exposure substantially promoted α-T release, achieving cumulative release rates of 71.73% and 74.49% respectively over 60 h. The nanoparticle system showed no adverse effects on healthy tomato leaf growth while forming a protective barrier that effectively suppressed fungal infection. Targeted delivery significantly enhanced fungicidal efficacy, with α-T NPs exhibiting an EC₅₀ of 0.419 mg/L compared to 1.943 mg/L for α-T (technical material, TC). Confocal microscopy and quantitative analysis confirmed the enhanced targeting mechanism, revealing substantially stronger fluorescence intensity and higher α-T accumulation specifically on treated fungal hyphae. Additionally, α-T NPs significantly improved photostability, extending the half-life by approximately 1.93-fold under continuous illumination compared to α-T (TC). This work establishes an innovative “pathogen-activated” delivery platform with promising applications in sustainable crop protection.

植物源性光活性杀菌剂α-Terthienyl （α-T）通过光诱导活性氧生成发挥抗菌作用。但其瞄准能力差、光稳定性低，制约了其在农业上的应用。为了解决这些挑战，我们开发了一种基于果胶-壳聚糖聚合物网络的双响应纳米颗粒递送系统（α-T NPs），该系统能够特异性识别葡萄孢菌丝分泌产生的酸性和富含果胶酶的微环境。控释研究表明，酸性条件（pH 5.0）和果胶酶暴露均能显著促进α-T的释放，60 h内α-T的累积释放率分别为71.73%和74.49%。纳米颗粒体系对健康番茄叶片生长无不良影响，同时形成了有效抑制真菌感染的保护屏障。靶向递送显著提高了杀真菌效果，α-T NPs的EC₅0为0.419 mg/L，而α-T（技术材料，TC）的EC₅0为1.943 mg/L。共聚焦显微镜和定量分析证实了靶向机制的增强，发现处理真菌菌丝的荧光强度明显增强，α-T积累明显增加。此外，α-T NPs显著提高了光稳定性，在连续光照下的半衰期比α-T （TC）延长了约1.93倍。这项工作建立了一个创新的“病原体激活”传递平台，在可持续作物保护中具有广阔的应用前景。

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引用次数: 0

Structure-function relationship of polysaccharides derived from Pericarpium Citri Reticulatae 'Chachiensis': highlighting the effects on metabolic syndrome by regulating gut microbiota 柑桔皮多糖的结构-功能关系：通过调节肠道菌群对代谢综合征的影响

IF 12.5 1区化学 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymers

Pub Date : 2026-01-14 DOI: 10.1016/j.carbpol.2026.124936

Gang Huang , Bing Li , Chengguo Li , Baizhong Chen , Yapeng Li , Shuying Li , Lishe Gan , Dongli Li , Lei Chen , Chengwei He , Xueling Zhang , Ren-You Gan , Rihui Wu

The precise interaction between gut microbes and dietary polysaccharides is not fully understood. This study elucidated the structure-function relationship and the underlying mechanisms of polysaccharides derived from Pericarpium Citri Reticulatae ‘Chachiensis’ (PCRCP) against a high-fat diet (HFD)-induced metabolic syndrome (MetS). Three subfractions (PCRCPI-III) were isolated, with GalA contents of 79.7%, 56.7%, and 33.5% and average molecular weights of 48.85, 32.28, and 51.12 kDa, respectively. Notably, PCRCPI exhibits a linear backbone composed of →4)-GalA-(1→ residues, complemented by side chains of →5)-Ara-(1→ and →4)-Gal-(1→, interconnected via →2,4)-Rha-(1→ linkages. Their efficacy in mitigating MetS was structure-dependent, with PCRCPI exerting the most significant therapeutic effects. Oral administration of PCRCPI in mice alleviated metabolic phenotypes in a gut microbiota-dependent manner, characterized by the selective enrichment of an elongation taxonomic chain Lactobacillales-Lactobacillaceae-Lactobacillus-Lactobacillus spp. Colonization with live Lactobacillus strains enhanced the efficacy of PCRCPI in improving metabolic phenotypes, especially when co-administered with Lactobacillus murinus, which synergistically augmented insulin sensitivity and activated hepatic PPAR signaling. Additionally, PCRCPI increased microbial-derived deoxycholic acid, which activated PPAR-mediated fatty acid oxidation in hepatocytes. These findings suggest that PCRCPI may serve as a promising therapeutic agent for MetS management, potentially through the targeted stimulation of beneficia Lactobacillus proliferation.

肠道微生物与膳食多糖之间的确切相互作用尚不完全清楚。本研究阐明了柑橘皮多糖（PCRCP）抗高脂饮食（HFD）诱导的代谢综合征（MetS）的结构-功能关系及其潜在机制。分离得到3个亚分（PCRCPI-III）， GalA含量分别为79.7%、56.7%和33.5%，平均分子量分别为48.85、32.28和51.12 kDa。值得注意的是，PCRCPI呈现出由→4)- gala -(1)→残基组成的线性主链，辅之以→5)- ara -(1→和→4)- gal -(1→侧链，通过→2,4)- rha -(1→键相互连接。它们缓解MetS的疗效是结构依赖的，其中PCRCPI发挥最显著的治疗效果。小鼠口服PCRCPI以肠道菌群依赖的方式缓解代谢表型，其特征是选择性富集延伸分类链乳酸杆菌-乳酸杆菌科-乳酸杆菌-乳杆菌属。与活乳杆菌菌株定殖可增强PCRCPI改善代谢表型的功效，特别是与乳酸菌共同给药时。协同增强胰岛素敏感性和激活肝脏PPAR信号。此外，PCRCPI增加微生物来源的脱氧胆酸，激活肝细胞中ppar介导的脂肪酸氧化。这些发现表明，PCRCPI可能作为一种有前途的治疗药物，用于MetS的管理，可能通过靶向刺激有益乳杆菌的增殖。

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