Bu çalışmada, ıhlamur (Tilia rubra subsp. caucasica) çiçek ektraktı indirgeyici ve kaplayıcı ajan olarak kullanılarak mikrodalga ile gümüş nanopartiküller (AgNP’ler) sentezlenmiştir. AgNP’lerin optimum üretim miktarını elde etmeyi amaçlayan dört farklı sentez değişkeninin birleşik etkisini araştırmak için Tepki Yüzey Metodolojisinin Yüz Merkezli Merkezi Kompozit Tasarımı kullanıldı. Araştırılan aralık içinde, AgNO3 konsantrasyonu, bitki ekstrakt miktarı, mikrodalga gücü ve zaman sırasıyla 10 mM, 2.5 ml, 800 watt ve 90 saniye olduğunda optimum AgNP üretimi elde edilmiştir. UV–Vis, FT–IR, SEM–EDS ve TEM, sentezlenen AgNP’leri karakterize etmek için kullanıldı. Ayrıca in vitro deneyler, yedi Phytophthora türü için sentezlenen AgNP’lerin LC50, MIC ve MFC değerlerinin sırasıyla 46.38-119.36 µg ml-1, 225-450 µg ml-1 ve 225-900 µg ml-1 arasında değiştiğini ortaya koymuştur. Bu çalışmanın sonuçları, ıhlamur çiçek ekstraktı ile sentezlenen AgNP’lerin Phytophthora hastalıklarının kontrolünde kullanım için daha fazla araştırılması gerektiğini göstermektedir.
{"title":"Antifungal activity and optimization procedure of microwave-synthesized silver nanoparticles using linden (Tilia rubra subsp. caucasica) flower extract","authors":"U. Yi̇ği̇t, M. Türkkan","doi":"10.32571/ijct.1194356","DOIUrl":"https://doi.org/10.32571/ijct.1194356","url":null,"abstract":"Bu çalışmada, ıhlamur (Tilia rubra subsp. caucasica) çiçek ektraktı indirgeyici ve kaplayıcı ajan olarak kullanılarak mikrodalga ile gümüş nanopartiküller (AgNP’ler) sentezlenmiştir. AgNP’lerin optimum üretim miktarını elde etmeyi amaçlayan dört farklı sentez değişkeninin birleşik etkisini araştırmak için Tepki Yüzey Metodolojisinin Yüz Merkezli Merkezi Kompozit Tasarımı kullanıldı. Araştırılan aralık içinde, AgNO3 konsantrasyonu, bitki ekstrakt miktarı, mikrodalga gücü ve zaman sırasıyla 10 mM, 2.5 ml, 800 watt ve 90 saniye olduğunda optimum AgNP üretimi elde edilmiştir. UV–Vis, FT–IR, SEM–EDS ve TEM, sentezlenen AgNP’leri karakterize etmek için kullanıldı. Ayrıca in vitro deneyler, yedi Phytophthora türü için sentezlenen AgNP’lerin LC50, MIC ve MFC değerlerinin sırasıyla 46.38-119.36 µg ml-1, 225-450 µg ml-1 ve 225-900 µg ml-1 arasında değiştiğini ortaya koymuştur. Bu çalışmanın sonuçları, ıhlamur çiçek ekstraktı ile sentezlenen AgNP’lerin Phytophthora hastalıklarının kontrolünde kullanım için daha fazla araştırılması gerektiğini göstermektedir.","PeriodicalId":267255,"journal":{"name":"International Journal of Chemistry and Technology","volume":"97 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131400722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. Ulusal, F. Ulusal, Mehmet Akif Bozdayi, Bilgehan Güzel, S. Taysı, M. Tarakçıoğlu
Idarubicin is a chemotherapeutic drug frequently used to treat breast cancer and acute leukemia. This study aimed to immobilize idarubicin on glutaraldehyde (GA)-coated magnetic nanoparticles (MNP-GA) to prepare a drug with high stability and low toxicity. We prefreed MNPS because of their easy synthesis, low cost, and non-toxicity. In the study, magnetite (Fe3O4) nanoparticles were prepared, coated with glutaraldehyde, characterization processes were performed with Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction pattern (XRD), and Conventional transmission electron microscopy (C-TEM) methods, and idarubicin (IDA) was bound. The cytotoxic effects of idarubicin-bound MNP-GA and free idarubicin on HL-60 cell lines were determined by MTT and ATP tests, and IC50 values were calculated. Flow cytometry was used to evaluate apoptosis status, and the expression of MDR1, Puma, NOXA, BAX, Survivin, and BCL-2 genes were measured by the polymerase chain reaction (PCR). It was found that the IC50 decreased between 5 and 7 times with the use of MNP. In PCR tests, the expressions of apoptotic genes increased, while the expressions of MDR1 and anti-apoptotic genes were decreased in the use of MNP. Apoptosis was found to be increased in flow cytometry measurements. The use of MNP systems has reduced drug resistance since it provides controlled release of the drug and prevents its exit from the cell due to its structure.
{"title":"Attachment of Idarubicin to Glutaraldehyde-coated Magnetic Nanoparticle and Investigation of its Effect in HL-60 Cell Line","authors":"H. Ulusal, F. Ulusal, Mehmet Akif Bozdayi, Bilgehan Güzel, S. Taysı, M. Tarakçıoğlu","doi":"10.32571/ijct.1194667","DOIUrl":"https://doi.org/10.32571/ijct.1194667","url":null,"abstract":"Idarubicin is a chemotherapeutic drug frequently used to treat breast cancer and acute leukemia. This study aimed to immobilize idarubicin on glutaraldehyde (GA)-coated magnetic nanoparticles (MNP-GA) to prepare a drug with high stability and low toxicity. We prefreed MNPS because of their easy synthesis, low cost, and non-toxicity. In the study, magnetite (Fe3O4) nanoparticles were prepared, coated with glutaraldehyde, characterization processes were performed with Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction pattern (XRD), and Conventional transmission electron microscopy (C-TEM) methods, and idarubicin (IDA) was bound. The cytotoxic effects of idarubicin-bound MNP-GA and free idarubicin on HL-60 cell lines were determined by MTT and ATP tests, and IC50 values were calculated. Flow cytometry was used to evaluate apoptosis status, and the expression of MDR1, Puma, NOXA, BAX, Survivin, and BCL-2 genes were measured by the polymerase chain reaction (PCR). It was found that the IC50 decreased between 5 and 7 times with the use of MNP. In PCR tests, the expressions of apoptotic genes increased, while the expressions of MDR1 and anti-apoptotic genes were decreased in the use of MNP. Apoptosis was found to be increased in flow cytometry measurements. The use of MNP systems has reduced drug resistance since it provides controlled release of the drug and prevents its exit from the cell due to its structure.","PeriodicalId":267255,"journal":{"name":"International Journal of Chemistry and Technology","volume":"78 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121553977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
As a result of the rapid increase in the world population, the problem of balanced and adequate nutrition has emerged, and at this point, the importance of animal production has increased even more. In this respect, there is a need to increase the cultivation areas of forage crops, as well as the correct management and reclamation of pastures for adequate and balanced nutrition of our existing animals. For this reason, forage pea (Pisum sativum L.), which has high nutritional value and is preferred by animals, is an important forage crop to meet this need. Salinity significantly limits the growth of the plant and causes yield losses. This abiotic stress negatively affects forage peas. In order to reveal the differences in the germination of forage pea varieties at different salt concentrations and the differences in the physical properties of the roots and seedlings, the effect of different salt concentrations on nine different varieties of forage peas was carried out in Bilecik Şeyh Edebali University, Faculty of Agriculture and Natural Sciences, Agricultural Research and Biotechnology Center Laboratories in 2022. In the research, the effects of nine different field types (Ürünlü, Töre, Ateş, Guifredo, Taşkent, Özkaynak, Kurtbey, Uysal and Nany) at one control and eight different salt concentrations (25, 50, 75, 100, 125, 150, 175 and 200 mM) on germination and seedling growth were examined. The experiment was carried out according to Completely Randomized Factorial Designs with 3 replications. In the research; germination speed and power, root dry and fresh weight, root dry and fresh length, seedling dry and fresh weight, seedling dry and fresh length were examined. According to the results obtained, a significant decrease was observed in germination rate, germination strength, root-shoot length and wet-dry weights of forage pea varieties due to increasing salt concentration. It has been determined that Tore field pea variety has the highest germination rate and power. Tore field pea showed 100% germination speed and power up to 150 mM salt concentration. In addition, Töre variety is followed by Ateş variety in all parameters. The varieties with the lowest germination rate and vigour were Guifredo and Uysal. As a result, the growth of forage pea varieties slowed down due to increasing salt concentration.
由于世界人口的迅速增加，营养均衡和充足的问题出现了，在这一点上，动物生产的重要性更加增加了。在这方面，有必要增加饲料作物的种植面积，以及正确管理和开垦牧场，以使我们现有的动物获得充分和均衡的营养。因此，饲料豌豆(Pisum sativum L.)是满足这一需求的重要饲料作物，具有较高的营养价值，是动物的首选。盐度极大地限制了植物的生长并造成产量损失。这种非生物胁迫对饲料豌豆产生不利影响。为了揭示不同盐浓度下饲用豌豆品种发芽的差异以及根系和幼苗物理特性的差异，于2022年在Bilecik Şeyh Edebali大学农业与自然科学学院农业研究与生物技术中心实验室开展了不同盐浓度对9个不同品种饲用豌豆的影响。研究了9种不同田间类型(Ürünlü、Töre、atezu、Guifredo、takent、Özkaynak、Kurtbey、Uysal和Nany)在1种对照和8种不同盐浓度(25、50、75、100、125、150、175和200 mM)下对油菜发芽和幼苗生长的影响。试验采用完全随机因子设计，共3个重复。在研究中;测定萌发速度和萌发力、根干鲜重、根干鲜长、幼苗干鲜重、幼苗干鲜长。结果表明，随着盐浓度的升高，饲用豌豆品种的发芽率、发芽强度、根茎长和干湿重均显著降低。经测定，大田豌豆品种的发芽率和发芽力最高。油菜在150mm盐浓度下萌发率为100%。此外，在所有参数中，Töre品种之后是atekat品种。发芽率和活力最低的品种是吉弗雷多和尤萨。结果表明，由于盐浓度的增加，饲用豌豆品种的生长速度减慢。
{"title":"Effect of Different Salt Concentrations on Germination of Forage Pea","authors":"Nilay Kayın, Ferzat Turan, Emine Serap Aydemi̇r","doi":"10.32571/ijct.1171249","DOIUrl":"https://doi.org/10.32571/ijct.1171249","url":null,"abstract":"As a result of the rapid increase in the world population, the problem of balanced and adequate nutrition has emerged, and at this point, the importance of animal production has increased even more. In this respect, there is a need to increase the cultivation areas of forage crops, as well as the correct management and reclamation of pastures for adequate and balanced nutrition of our existing animals. For this reason, forage pea (Pisum sativum L.), which has high nutritional value and is preferred by animals, is an important forage crop to meet this need. Salinity significantly limits the growth of the plant and causes yield losses. This abiotic stress negatively affects forage peas. In order to reveal the differences in the germination of forage pea varieties at different salt concentrations and the differences in the physical properties of the roots and seedlings, the effect of different salt concentrations on nine different varieties of forage peas was carried out in Bilecik Şeyh Edebali University, Faculty of Agriculture and Natural Sciences, Agricultural Research and Biotechnology Center Laboratories in 2022. In the research, the effects of nine different field types (Ürünlü, Töre, Ateş, Guifredo, Taşkent, Özkaynak, Kurtbey, Uysal and Nany) at one control and eight different salt concentrations (25, 50, 75, 100, 125, 150, 175 and 200 mM) on germination and seedling growth were examined. The experiment was carried out according to Completely Randomized Factorial Designs with 3 replications. In the research; germination speed and power, root dry and fresh weight, root dry and fresh length, seedling dry and fresh weight, seedling dry and fresh length were examined. According to the results obtained, a significant decrease was observed in germination rate, germination strength, root-shoot length and wet-dry weights of forage pea varieties due to increasing salt concentration. It has been determined that Tore field pea variety has the highest germination rate and power. Tore field pea showed 100% germination speed and power up to 150 mM salt concentration. In addition, Töre variety is followed by Ateş variety in all parameters. The varieties with the lowest germination rate and vigour were Guifredo and Uysal. As a result, the growth of forage pea varieties slowed down due to increasing salt concentration.","PeriodicalId":267255,"journal":{"name":"International Journal of Chemistry and Technology","volume":"3 5","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"120927191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
LiBeH3 has been considered as a solid-state hydrogen storage material. This study investigated Pnma orthorhombic phase of LiBeH3 under pressure. Ab initio constant pressure molecular dynamic simulation under pressure was adopted. The results depicted a phase transition from Pnma orthorhombic phase to P21/m monoclinic phase at 270 GPa simulation pressure. The stability of each phase was examined using elastic constants. Based on the well-known Born stability criteria, both phases showed mechanical stability. Several moduli have been computed via elastic constants. The B/G ratios, Cauchy pressures and Poisson’s ratios investigation revealed that LiBeH3 is brittle at Pnma phase whereas it is ductile at P21/m phase. The electronic band structures and partial and total density of states of phases were also obtained. A 2.058 eV band gap was seen for Pnma phase, and 3 eV band gap was seen for P21/m phase.
{"title":"Structural transition of LiBeH3 under high pressure","authors":"Ç. Yamçıçıer, S. Al","doi":"10.32571/ijct.1190931","DOIUrl":"https://doi.org/10.32571/ijct.1190931","url":null,"abstract":"LiBeH3 has been considered as a solid-state hydrogen storage material. This study investigated Pnma orthorhombic phase of LiBeH3 under pressure. Ab initio constant pressure molecular dynamic simulation under pressure was adopted. The results depicted a phase transition from Pnma orthorhombic phase to P21/m monoclinic phase at 270 GPa simulation pressure. The stability of each phase was examined using elastic constants. Based on the well-known Born stability criteria, both phases showed mechanical stability. Several moduli have been computed via elastic constants. The B/G ratios, Cauchy pressures and Poisson’s ratios investigation revealed that LiBeH3 is brittle at Pnma phase whereas it is ductile at P21/m phase. The electronic band structures and partial and total density of states of phases were also obtained. A 2.058 eV band gap was seen for Pnma phase, and 3 eV band gap was seen for P21/m phase.","PeriodicalId":267255,"journal":{"name":"International Journal of Chemistry and Technology","volume":"29 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116354371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Numerical simulations are carried out to understand the heat energy transport characteristics of microchannel reactors for hydrogen production by steam-methanol reforming on copper-based catalysts. Enthalpy analysis is performed and the evolution of energy in the oxidation and reforming processes is discussed in terms of reaction heat flux. The effects of solid thermal conductivity, gas velocity, and flow arrangement on the thermal behavior of the reactor is evaluated in order to fully describe the thermal energy change in the reactor. The results indicate that the thermal behavior of the reactor depends upon the thermal properties of the walls. The change in enthalpy is of particular importance in exothermic and endothermic reactions. The net enthalpy change for oxidation and reforming is negative and positive, but the net sensible enthalpy change is always positive in the reactor. The wall heat conduction effect accompanying temperature changes is important to the autothermal design and self-sustaining operation of the reactor. The solid thermal conductivity is of great importance in determining the operation and efficiency of the reactor. The reaction proceeds rapidly and efficiently only at high solid thermal conductivity. The reaction heat flux for oxidation and reforming is positive and negative. The change in flow arrangement significantly affects the reaction heat flux in the reactor. The parallel flow design is advantageous for purposes of enhancing heat transfer and avoiding localized hot spots.
{"title":"Heat energy transport characteristics of microchannel reactors for hydrogen production by steam-methanol reforming on copper-based catalysts","authors":"Junjie Chen","doi":"10.32571/ijct.1060520","DOIUrl":"https://doi.org/10.32571/ijct.1060520","url":null,"abstract":"Numerical simulations are carried out to understand the heat energy transport characteristics of microchannel reactors for hydrogen production by steam-methanol reforming on copper-based catalysts. Enthalpy analysis is performed and the evolution of energy in the oxidation and reforming processes is discussed in terms of reaction heat flux. The effects of solid thermal conductivity, gas velocity, and flow arrangement on the thermal behavior of the reactor is evaluated in order to fully describe the thermal energy change in the reactor. The results indicate that the thermal behavior of the reactor depends upon the thermal properties of the walls. The change in enthalpy is of particular importance in exothermic and endothermic reactions. The net enthalpy change for oxidation and reforming is negative and positive, but the net sensible enthalpy change is always positive in the reactor. The wall\u0000heat conduction effect accompanying temperature changes is important to the autothermal design and self-sustaining operation of the reactor. The solid thermal conductivity is of great importance in determining the operation and efficiency of the reactor. The reaction proceeds rapidly and efficiently only at high solid thermal conductivity. The reaction heat flux for oxidation and reforming is positive and negative. The change in flow arrangement significantly affects the reaction heat flux in the reactor. The parallel flow design is advantageous for purposes of enhancing heat transfer and avoiding localized hot spots.","PeriodicalId":267255,"journal":{"name":"International Journal of Chemistry and Technology","volume":"65 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122620643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nanotechnology has gained great interest due to its widespread application. Anticancer activity of Origanum majorana extract and silver nanoparticles was investigated by MTT assay using human pancreatic adenocarcinoma cell lines (Capan-1), mouse normal fibroblast cell lines (L929), and human colon adenocarcinoma cell line (Caco-2). AgNPs@Om exhibited the excellent cytotoxic effect on Capan-1 cell lines with the cell viability of 29.17% and 25.70%, 22.49%, 21.16% at 1.0, 0.5, 0.25, 0.125 µg/mL respectively. However, the extract was determined to show moderate activity. A considerable fall in the viable cell number in Caco-2 cell lines was observed after the treatment of AgNPs@Om. The viable cells of Caco-2 cell lines were detected as 32.0% at 1.0 µg/mL with the treatment of nanoparticles. Yet, the viable Caco-2 cells were determined as 48.2% with the treatment of O. majorana extract. Both extract and nanoparticles had no impact on the viability of non-tumor cells (L929). Moreover, nanoparticles induced apoptosis. Consequently, AgNPs@Om may be a promising anticancer drug candidate.
{"title":"Cytotoxic, apoptotic, and necrotic effects of silver nanoparticles biosynthesized using Origanum majorana extract","authors":"R. Erenler, E. Gecer, Büşra MORAN BOZER","doi":"10.32571/ijct.1187754","DOIUrl":"https://doi.org/10.32571/ijct.1187754","url":null,"abstract":"Nanotechnology has gained great interest due to its widespread application. Anticancer activity of Origanum majorana extract and silver nanoparticles was investigated by MTT assay using human pancreatic adenocarcinoma cell lines (Capan-1), mouse normal fibroblast cell lines (L929), and human colon adenocarcinoma cell line (Caco-2). AgNPs@Om exhibited the excellent cytotoxic effect on Capan-1 cell lines with the cell viability of 29.17% and 25.70%, 22.49%, 21.16% at 1.0, 0.5, 0.25, 0.125 µg/mL respectively. However, the extract was determined to show moderate activity. A considerable fall in the viable cell number in Caco-2 cell lines was observed after the treatment of AgNPs@Om. The viable cells of Caco-2 cell lines were detected as 32.0% at 1.0 µg/mL with the treatment of nanoparticles. Yet, the viable Caco-2 cells were determined as 48.2% with the treatment of O. majorana extract. Both extract and nanoparticles had no impact on the viability of non-tumor cells (L929). Moreover, nanoparticles induced apoptosis. Consequently, AgNPs@Om may be a promising anticancer drug candidate.","PeriodicalId":267255,"journal":{"name":"International Journal of Chemistry and Technology","volume":"22 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128707828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Fenantren-9-karbaldehit ve 4-aminofenolün kondenzasyon reaksiyonu ile fenantren bazlı yeni bir Schiff baz bileşiği (PBS) sentezlenmiştir. Bileşiğin yapısı FTIR, 1H(13C) NMR ve elementel analizi ile karakterize edildi. Bileşiğin kristal yapısı, tek kristal X-ışını kırınım deneyi ile belirlendi. X-ışını kristalografik verileri, fenantren ve fenol halkalarının birbirine göre yaklaşık olarak dik olduğunu ortaya çıkardı. Yapıda moleküller, moleküller arası fenol-imin hidrojen bağları [O1-H·····N1] ile bağlanır ve supramoleküler hidrojen bağ zincirleri oluşturur. Ayrıca fenantren halka sistemi, komşu moleküllerin aynı bölümleri ile π-π istifleme etkil (baş-kuruk) etkileşimlerinde yer alır. Bileşiğin çift sarmallı balık sperm DNA'sına (dsFS-DNA) bağlanma özellikleri spektrofotometrik, florimetrik ve viskosimetrik yöntemlerle araştırılmıştır. Spektral veriler, bileşiğin, önemli bağlanma sabiti (Kb: 4.6 x 104 M-1) ile oluk bağlanma modunda DNA ile etkileşime girdiğini göstermiştir. Bileşik PBS'nin varlığında, dsFS-DNA'nın viskozitesi önemli bir değişiklik gözlenmemesi, bileşiğin dsFS-DNA ile interkalatif olmayan bağlanma modunda etkileşime girdiğini göstermektedir.
{"title":"Structural characterization and DNA binding properties of a phenanthrene based Schiff base compound","authors":"A. Köse","doi":"10.32571/ijct.1170911","DOIUrl":"https://doi.org/10.32571/ijct.1170911","url":null,"abstract":"Fenantren-9-karbaldehit ve 4-aminofenolün kondenzasyon reaksiyonu ile fenantren bazlı yeni bir Schiff baz bileşiği (PBS) sentezlenmiştir. Bileşiğin yapısı FTIR, 1H(13C) NMR ve elementel analizi ile karakterize edildi. Bileşiğin kristal yapısı, tek kristal X-ışını kırınım deneyi ile belirlendi. X-ışını kristalografik verileri, fenantren ve fenol halkalarının birbirine göre yaklaşık olarak dik olduğunu ortaya çıkardı. Yapıda moleküller, moleküller arası fenol-imin hidrojen bağları [O1-H·····N1] ile bağlanır ve supramoleküler hidrojen bağ zincirleri oluşturur. Ayrıca fenantren halka sistemi, komşu moleküllerin aynı bölümleri ile π-π istifleme etkil (baş-kuruk) etkileşimlerinde yer alır. Bileşiğin çift sarmallı balık sperm DNA'sına (dsFS-DNA) bağlanma özellikleri spektrofotometrik, florimetrik ve viskosimetrik yöntemlerle araştırılmıştır. Spektral veriler, bileşiğin, önemli bağlanma sabiti (Kb: 4.6 x 104 M-1) ile oluk bağlanma modunda DNA ile etkileşime girdiğini göstermiştir. Bileşik PBS'nin varlığında, dsFS-DNA'nın viskozitesi önemli bir değişiklik gözlenmemesi, bileşiğin dsFS-DNA ile interkalatif olmayan bağlanma modunda etkileşime girdiğini göstermektedir.","PeriodicalId":267255,"journal":{"name":"International Journal of Chemistry and Technology","volume":"14 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114313586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tchihatchewia isatidea Boiss (Brassicaceae) is popularly known as “Paint and Bridal Flower”. Flower pieces and extracts can be used for painting, wound healing, and cough treatments. In this study, the nutritional and medicinal properties of T. isidea were determined. For this purpose, carbohydrate, crude oil, crude protein, crude cellulose, energy, crude ash, dry matter, moisture, element, vitamin, antioxidant capacity, DNA protective, and antimicrobial effects of T. isatidea were investigated. Crude ash, crude protein, crude fat, crude cellulose, carbohydrate, dry matter, energy, organic matter, K, Ca, Na, Fe, Cu, Zn, Pb, Mn, vitamin E and vitamin A paremeters of T. Isatidea were 14.37%, 20.60%, 1.82%, 29.02%, 63.21%, 91.57%, 351.62 kcal 72.20%, 42.5 mg/kg, 102.4 mg/kg, 260 mg/kg, 1.59 mg/kg, 0.121 mg/kg, 1.160 mg/kg, 2.16 mg/kg, 7.49 mg/kg, 22.95 mg/g and 2.82 mg/g, respectively. The DPPH radical scavenging activity of the plant extract was ’82.34% at 100 µL, 68.20% at 50 µL, 30.80% at 25 µL and 11.68% at 10 µL’. The total oxidant level was 69.96 μmol/L, the total antioxidant level of T. isatidea was 3.91 μmol/L and 133.56 nmol/g MDA. In the existence of UV and H2O2, it was found that the T. isatidea plant extract protects scDNA. It has also been established that the flower extract of the plant hinders the growth of pathogenic microorganisms at varying rates. Because of its thorny structure, the plant which can not be used as a vegetable can be transformed into a dried powdered form of powder and can be consumed with water in the form of a decoction or infusion. The plant can be mixed with powdered petroleum jelly and used as an external wound healing agent.
{"title":"Nutritive Value, Antimicrobial and Antioxidant Activities of Tchihatchewia isatidea Boiss","authors":"Mürşide Gizem Bi̇rcan, S. Kırbağ, B. Bircan","doi":"10.32571/ijct.1146979","DOIUrl":"https://doi.org/10.32571/ijct.1146979","url":null,"abstract":"Tchihatchewia isatidea Boiss (Brassicaceae) is popularly known as “Paint and Bridal Flower”. Flower pieces and extracts can be used for painting, wound healing, and cough treatments. In this study, the nutritional and medicinal properties of T. isidea were determined. For this purpose, carbohydrate, crude oil, crude protein, crude cellulose, energy, crude ash, dry matter, moisture, element, vitamin, antioxidant capacity, DNA protective, and antimicrobial effects of T. isatidea were investigated. Crude ash, crude protein, crude fat, crude cellulose, carbohydrate, dry matter, energy, organic matter, K, Ca, Na, Fe, Cu, Zn, Pb, Mn, vitamin E and vitamin A paremeters of T. Isatidea were 14.37%, 20.60%, 1.82%, 29.02%, 63.21%, 91.57%, 351.62 kcal 72.20%, 42.5 mg/kg, 102.4 mg/kg, 260 mg/kg, 1.59 mg/kg, 0.121 mg/kg, 1.160 mg/kg, 2.16 mg/kg, 7.49 mg/kg, 22.95 mg/g and 2.82 mg/g, respectively. The DPPH radical scavenging activity of the plant extract was ’82.34% at 100 µL, 68.20% at 50 µL, 30.80% at 25 µL and 11.68% at 10 µL’. The total oxidant level was 69.96 μmol/L, the total antioxidant level of T. isatidea was 3.91 μmol/L and 133.56 nmol/g MDA. In the existence of UV and H2O2, it was found that the T. isatidea plant extract protects scDNA. It has also been established that the flower extract of the plant hinders the growth of pathogenic microorganisms at varying rates. Because of its thorny structure, the plant which can not be used as a vegetable can be transformed into a dried powdered form of powder and can be consumed with water in the form of a decoction or infusion. The plant can be mixed with powdered petroleum jelly and used as an external wound healing agent.","PeriodicalId":267255,"journal":{"name":"International Journal of Chemistry and Technology","volume":"49 9 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126123254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Collagen, the main protein of the body, is extracted in different forms and used as reservoir for drug delivery. The aim of this work was to obtain adrug delivery systems based on collagen-dextran matrices cross-linked with glutaraldehyde as support and flufenamic acid and/or microcapsules with flufenamic acid as drug. The flufenamic acid was encapsulated in polymeric microcapsules consisting in gelatin, alginate and sodium carboxymethyl cellulose. The morphology of matrices was determined by water absorption and contact angle. The biodegradation was performed in collagenase solution. In vitro flufenamic acid release profiles were built and the kinetic mechanism was set according to different mathematical models. The pharmacological studies followed the effect of collagen formulations treatment on the healing process of Wistar rats which were induced experimental wounds. The studied matrices proved that flufenamic acid delivery can be controlled, and the healing can be completed using the designed spongious matrices.
{"title":"Polymer – flufenamic acid delivery systems for injured skin","authors":"M. A. Albu Kaya","doi":"10.32571/ijct.1135548","DOIUrl":"https://doi.org/10.32571/ijct.1135548","url":null,"abstract":"Collagen, the main protein of the body, is extracted in different forms and used as reservoir for drug delivery. The aim of this work was to obtain adrug delivery systems based on collagen-dextran matrices cross-linked with glutaraldehyde as support and flufenamic acid and/or microcapsules with flufenamic acid as drug. The flufenamic acid was encapsulated in polymeric microcapsules consisting in gelatin, alginate and sodium carboxymethyl cellulose. The morphology of matrices was determined by water absorption and contact angle. The biodegradation was performed in collagenase solution. In vitro flufenamic acid release profiles were built and the kinetic mechanism was set according to different mathematical models. The pharmacological studies followed the effect of collagen formulations treatment on the healing process of Wistar rats which were induced experimental wounds. The studied matrices proved that flufenamic acid delivery can be controlled, and the healing can be completed using the designed spongious matrices.","PeriodicalId":267255,"journal":{"name":"International Journal of Chemistry and Technology","volume":"55 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124639249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Of late, the automobiles of all categories are massively increasing which aggravates the dependency on fossil fuel thus, the accessibility of petro-fuel decreases relentlessly. Under these circumstances, the investigations are taking new dimensions to find alternative, renewable with cost-effective fuel exclusively biofuels. However, a newer approach has been attempted for the production of biodiesel from wild stuff of Spirulina algae which is explored directly in its aquatic systems of the natural environment. The chemical extraction process was attempted with wild biomass of algae via pre-treatment for obtaining promising lipid content then; the algal oil (lipid) was subjected to the production of biodiesel through sequential transesterification reactions. The obtained biodiesel was measured as 94.65% produced from 0.920kg of algal oil extracted out of 1.6kg of algal biomass in a stipulated duration of time (2.50hr) taken for attaining maximum temperature. Further, the produced biodiesel was evaluated for its distinctive properties and the outcomes were compared with ASTM standard specifications. All the results appeared to be within the standards limit thereby, the methodical analysis was done for the performance and emission parameters via interacting algal biodiesel in the experimental (DI) diesel engine test-rig configured with a variable injection pressure of 210, 220 & 230 respectively. The blending ratio of algal diesel and gasoline (AB10 & AB 20v/v) were prepared based on volume and the results were correlated with petro-diesel. The physico-chemical parameters like color, odour, viscosity, density, total acid number, initial boiling point, flash point, calorific value, cetane number etc. were found to be significant when compared with petro-fuel. Correspondingly, the interactive parameters like flash point, pour point, cloud point, carbon residue, sulfur, ash, and water contents were correlated with the standard specifications. The substantial approach was observed in emission characteristics such as hydrocarbon (HC), carbon monoxide (CO), oxides of nitrogen (NOX) respectively in engine-algal fuel interactions. Whereas, a slight increase than petro-diesel with respect to sulfur oxide (SOX) emission and smoke opacity were also recorded. However, the marginal increase in Brake specific fuel consumption (BSFC) and Brake thermal efficiency (BTE) was noticed and the brake power (BP) was found slightly lower than petro-diesel. In conclusion, the protocol of producing biodiesel from wild stuff biomass of Spirulina maxima was found to be techno-economically feasible and this potential algal biodiesel can be recommended for the current engine applications without any alterations.
{"title":"Chemical extraction of Biodiesel and Parametric analysis of DI-Diesel Engine using Algae esterified Oil-Gasoline Blends","authors":"R. Kumar, P. MURTHY G","doi":"10.32571/ijct.1105415","DOIUrl":"https://doi.org/10.32571/ijct.1105415","url":null,"abstract":"Of late, the automobiles of all categories are massively increasing which aggravates the dependency on fossil fuel thus, the accessibility of petro-fuel decreases relentlessly. Under these circumstances, the investigations are taking new dimensions to find alternative, renewable with cost-effective fuel exclusively biofuels. However, a newer approach has been attempted for the production of biodiesel from wild stuff of Spirulina algae which is explored directly in its aquatic systems of the natural environment. The chemical extraction process was attempted with wild biomass of algae via pre-treatment for obtaining promising lipid content then; the algal oil (lipid) was subjected to the production of biodiesel through sequential transesterification reactions. The obtained biodiesel was measured as 94.65% produced from 0.920kg of algal oil extracted out of 1.6kg of algal biomass in a stipulated duration of time (2.50hr) taken for attaining maximum temperature. Further, the produced biodiesel was evaluated for its distinctive properties and the outcomes were compared with ASTM standard specifications. All the results appeared to be within the standards limit thereby, the methodical analysis was done for the performance and emission parameters via interacting algal biodiesel in the experimental (DI) diesel engine test-rig configured with a variable injection pressure of 210, 220 & 230 respectively. The blending ratio of algal diesel and gasoline (AB10 & AB 20v/v) were prepared based on volume and the results were correlated with petro-diesel. The physico-chemical parameters like color, odour, viscosity, density, total acid number, initial boiling point, flash point, calorific value, cetane number etc. were found to be significant when compared with petro-fuel. Correspondingly, the interactive parameters like flash point, pour point, cloud point, carbon residue, sulfur, ash, and water contents were correlated with the standard specifications. The substantial approach was observed in emission characteristics such as hydrocarbon (HC), carbon monoxide (CO), oxides of nitrogen (NOX) respectively in engine-algal fuel interactions. Whereas, a slight increase than petro-diesel with respect to sulfur oxide (SOX) emission and smoke opacity were also recorded. However, the marginal increase in Brake specific fuel consumption (BSFC) and Brake thermal efficiency (BTE) was noticed and the brake power (BP) was found slightly lower than petro-diesel. In conclusion, the protocol of producing biodiesel from wild stuff biomass of Spirulina maxima was found to be techno-economically feasible and this potential algal biodiesel can be recommended for the current engine applications without any alterations.","PeriodicalId":267255,"journal":{"name":"International Journal of Chemistry and Technology","volume":"50 8","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"113974086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：