Pub Date : 2025-10-01DOI: 10.1016/j.cjac.2025.100620
DU Qing
The key active ingredients of Chinese medicinal materials are often used as indicators in quality standards. Corydalis conspersa is a characteristic plant from the Qinghai-Tibet plateau and contains various of isoquinoline alkaloids. Water content was determinated from diverse parts of Corydalis conspersa according to the Chinese Pharmacopoeia. The component of coptisine and berberine hydrochloride in Corydalis conspersa were extracted using the solvent of methanol-hydrochloric acid and detected by high-performance liquid chromatography. The results of method validation test was stable and reliable. The content of coptisine and berberine hydrochloride in root samples was higher than in herbs. In the sample solutions of stem and leaf, only coptisine was detectable. Thus, the levels of coptisine and berberine hydrochloride in root and herbs can serve as a qualified basis for quality standards of Corydalis conspersa.
{"title":"Quantitative determination of coptisine and berberine hydrochloride in Corydalis conspersa by high-performance liquid chromatography and quality evaluation","authors":"DU Qing","doi":"10.1016/j.cjac.2025.100620","DOIUrl":"10.1016/j.cjac.2025.100620","url":null,"abstract":"<div><div>The key active ingredients of Chinese medicinal materials are often used as indicators in quality standards. <em>Corydalis conspersa</em> is a characteristic plant from the Qinghai-Tibet plateau and contains various of isoquinoline alkaloids. Water content was determinated from diverse parts of <em>Corydalis conspersa</em> according to the Chinese Pharmacopoeia. The component of coptisine and berberine hydrochloride in <em>Corydalis conspersa</em> were extracted using the solvent of methanol-hydrochloric acid and detected by high-performance liquid chromatography. The results of method validation test was stable and reliable. The content of coptisine and berberine hydrochloride in root samples was higher than in herbs. In the sample solutions of stem and leaf, only coptisine was detectable. Thus, the levels of coptisine and berberine hydrochloride in root and herbs can serve as a qualified basis for quality standards of <em>Corydalis conspersa</em>.</div></div>","PeriodicalId":277,"journal":{"name":"Chinese Journal of Analytical Chemistry","volume":"53 10","pages":"Article 100620"},"PeriodicalIF":1.3,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145216296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-09-28DOI: 10.1016/j.cjac.2025.100640
Mingxi XIA , Litao GE , Alexandr ROGACHEV , Zijian WANG , Huilin WANG
Antioxidant enzymes serve as crucial reactive oxygen species (ROS) scavenging systems in living organisms, capable of efficiently catalyzing the decomposition of excess ROS and playing pivotal roles in anti-aging processes and tumor therapy. However, natural enzymes face significant limitations in practical applications, including limited sources, poor stability, and high production costs. The catalytic mechanisms and precisely organized three-dimensional structures of natural antioxidant enzymes provide important biomimetic insights for designing and developing artificial nanozymes. Among these, cerium-based nanozymes exhibit superior antioxidant-like activity and reaction kinetics due to their unique Ce3+/Ce4+ redox pairs and surface oxygen vacancy structures, demonstrating promising application in biomedical fields. This review systematically summarizes the catalytic mechanisms and biomedical applications of cerium-based antioxidant nanozymes. Building upon the biomimetic catalytic principles of natural catalase, we analyze the structure-activity relationships and performance enhancement strategies of cerium-based antioxidant nanozymes. Furthermore, we highlight recent advances in biomedical applications of cerium-based antioxidant nanozymes and discuss current challenges and future opportunities for their future use.
{"title":"Recent advances in antioxidant cerium-based nanozymes: Catalytic mechanisms and bioanalysis/biomedical applications","authors":"Mingxi XIA , Litao GE , Alexandr ROGACHEV , Zijian WANG , Huilin WANG","doi":"10.1016/j.cjac.2025.100640","DOIUrl":"10.1016/j.cjac.2025.100640","url":null,"abstract":"<div><div>Antioxidant enzymes serve as crucial reactive oxygen species (ROS) scavenging systems in living organisms, capable of efficiently catalyzing the decomposition of excess ROS and playing pivotal roles in anti-aging processes and tumor therapy. However, natural enzymes face significant limitations in practical applications, including limited sources, poor stability, and high production costs. The catalytic mechanisms and precisely organized three-dimensional structures of natural antioxidant enzymes provide important biomimetic insights for designing and developing artificial nanozymes. Among these, cerium-based nanozymes exhibit superior antioxidant-like activity and reaction kinetics due to their unique Ce<sup>3+</sup>/Ce<sup>4+</sup> redox pairs and surface oxygen vacancy structures, demonstrating promising application in biomedical fields. This review systematically summarizes the catalytic mechanisms and biomedical applications of cerium-based antioxidant nanozymes. Building upon the biomimetic catalytic principles of natural catalase, we analyze the structure-activity relationships and performance enhancement strategies of cerium-based antioxidant nanozymes. Furthermore, we highlight recent advances in biomedical applications of cerium-based antioxidant nanozymes and discuss current challenges and future opportunities for their future use.</div></div>","PeriodicalId":277,"journal":{"name":"Chinese Journal of Analytical Chemistry","volume":"53 12","pages":"Article 100640"},"PeriodicalIF":1.3,"publicationDate":"2025-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145527517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-09-13DOI: 10.1016/j.cjac.2025.100632
Muhammad NAVEED , Muhammad ASIM , Tariq AZIZ , Mehak AYYAZ , Shumaila IBRAHIM , Muhammad Nouman MAJEED , Ayaz Ali KHAN , Ammena Y. BINSALEH , Nawal AL-HOSHANI , Maher S. ALWETHAYNANI , Fakhria A. AL-JOUFI , Deema FALLATAH
Streptococcus pyogenes (S. pyogenes), a pathogen responsible for various severe infections, including streptococcal toxic shock syndrome (STSS), poses a significant therapeutic challenge worldwide. This study aims to explore the structural and functional properties of the DNA polymerase III subunit beta of S. pyogenes, identify potential phytochemical inhibitors, and evaluate their drug-like properties using computational methods. The DNA polymerase III subunit beta of S. pyogenes was successfully characterized, and its interactions with 60 phytochemicals from the Apiaceae family were predicted. ADMET analysis indicated that several compounds possessed desirable properties, including good solubility and high drug-likeness. Alpha-carotene emerged as a lead candidate exhibiting promising binding affinity and favorable interactions in docking studies. Additionally, to enhance the potency of the target compound, fragment optimization was performed, resulting in the development of a novel lead compound. Toxicity analysis indicated a low risk for adverse effects. Moreover, molecular dynamics simulations revealed that Alpha-carotene and DNA polymerase-III maintained stability with RMSD values below 4.3 Å. RMSF analysis showed significant flexibility in specific regions, reflecting effective interactions and increased stability over time. Overall, this study provides a comprehensive assessment of the DNA polymerase III subunit beta of S. pyogenes and its interactions with potential phytochemical inhibitors. Alpha-carotene, in particular, demonstrates significant potential as a therapeutic agent against S. pyogenes infections. These findings require further experimental validation for the potential development of novel antimicrobial agents against S. pyogenes.
{"title":"Combining network pharmacology and computational approaches for screening of apiaceae-derived phytochemicals as inhibitors of DNA polymerase III in streptococcus pyogenes causing streptococcal toxic shock syndrome","authors":"Muhammad NAVEED , Muhammad ASIM , Tariq AZIZ , Mehak AYYAZ , Shumaila IBRAHIM , Muhammad Nouman MAJEED , Ayaz Ali KHAN , Ammena Y. BINSALEH , Nawal AL-HOSHANI , Maher S. ALWETHAYNANI , Fakhria A. AL-JOUFI , Deema FALLATAH","doi":"10.1016/j.cjac.2025.100632","DOIUrl":"10.1016/j.cjac.2025.100632","url":null,"abstract":"<div><div><em>Streptococcus pyogenes</em> (<em>S. pyogenes</em>), a pathogen responsible for various severe infections, including streptococcal toxic shock syndrome (STSS), poses a significant therapeutic challenge worldwide. This study aims to explore the structural and functional properties of the DNA polymerase III subunit beta of <em>S. pyogenes</em>, identify potential phytochemical inhibitors, and evaluate their drug-like properties using computational methods. The DNA polymerase III subunit beta of <em>S. pyogenes</em> was successfully characterized, and its interactions with 60 phytochemicals from the Apiaceae family were predicted. ADMET analysis indicated that several compounds possessed desirable properties, including good solubility and high drug-likeness. Alpha-carotene emerged as a lead candidate exhibiting promising binding affinity and favorable interactions in docking studies. Additionally, to enhance the potency of the target compound, fragment optimization was performed, resulting in the development of a novel lead compound. Toxicity analysis indicated a low risk for adverse effects. Moreover, molecular dynamics simulations revealed that Alpha-carotene and DNA polymerase-III maintained stability with RMSD values below 4.3 Å. RMSF analysis showed significant flexibility in specific regions, reflecting effective interactions and increased stability over time. Overall, this study provides a comprehensive assessment of the DNA polymerase III subunit beta of <em>S. pyogenes</em> and its interactions with potential phytochemical inhibitors. Alpha-carotene, in particular, demonstrates significant potential as a therapeutic agent against <em>S. pyogenes</em> infections. These findings require further experimental validation for the potential development of novel antimicrobial agents against <em>S. pyogenes</em>.</div></div>","PeriodicalId":277,"journal":{"name":"Chinese Journal of Analytical Chemistry","volume":"53 11","pages":"Article 100632"},"PeriodicalIF":1.3,"publicationDate":"2025-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145334158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-09-11DOI: 10.1016/j.cjac.2025.100630
Xiaodong WANG , Zonghui LIU , Yinuo YAN , Minglu LI , Hongle AN , Hui WANG , Bingchun XUE
Unraveling the intrinsic correlation between disease and mitochondrial viscosity presents a significant challenge. Traditional mitochondrial viscosity probes, which depend on mitochondrial membrane potential (MMP), within the context of disease-related physiology and pathology, the decline of MMP in response to varying biological conditions can diminish the electrostatic forces binding probes to mitochondria. As a result, these probes are susceptible to diffusing away from the mitochondrial matrix, leading to unreliable detection outcomes and spatiotemporal data. To date, the visualization of abnormal mitochondrial viscosity in clinical samples from cancer patients has not been achieved. To overcome this pressing challenge, we have meticulously synthesized a potent mitochondrial immobilization and viscosity-sensitive fluorescent probe bearing a lengthy alkyl chain (M-KZ-C8). This probe comprises a carbazole moiety serving as an electron donor (D) and an indole group functioning as an electron acceptor (A), thus adopting a classical d-A molecular structure. Utilizing the twisted intramolecular charge transfer, M-KZ-C8 exhibits exceptional sensitivity to viscosity fluctuations. Moreover, we have observed that M-KZ-C8 possesses an impressive capability for precisely monitoring mitochondrial viscosity changes within living cells, unaffected by alterations in MMP. Leveraging M-KZ-C8, we have successfully imaged heightened mitochondrial viscosity in fatty liver and cancerous tissues. Remarkably, the probe has achieved the visualization of mitochondrial viscosity not only at tissue and organ levels but also in surgical specimens from clinical cancer patients for the first time, heralding its vast potential for clinical diagnosis of human cancers. These attributes render M-KZ-C8 an efficacious diagnostic tool for the precise detection, unwavering fidelity, and prolonged imaging of diseases associated with mitochondrial viscosity.
{"title":"Rational design of MMP-independent fluorescent probe for in vivo visualization of mitochondrial viscosity in fatty liver and cancer models","authors":"Xiaodong WANG , Zonghui LIU , Yinuo YAN , Minglu LI , Hongle AN , Hui WANG , Bingchun XUE","doi":"10.1016/j.cjac.2025.100630","DOIUrl":"10.1016/j.cjac.2025.100630","url":null,"abstract":"<div><div>Unraveling the intrinsic correlation between disease and mitochondrial viscosity presents a significant challenge. Traditional mitochondrial viscosity probes, which depend on mitochondrial membrane potential (MMP), within the context of disease-related physiology and pathology, the decline of MMP in response to varying biological conditions can diminish the electrostatic forces binding probes to mitochondria. As a result, these probes are susceptible to diffusing away from the mitochondrial matrix, leading to unreliable detection outcomes and spatiotemporal data. To date, the visualization of abnormal mitochondrial viscosity in clinical samples from cancer patients has not been achieved. To overcome this pressing challenge, we have meticulously synthesized a potent mitochondrial immobilization and viscosity-sensitive fluorescent probe bearing a lengthy alkyl chain (M-KZ-C8). This probe comprises a carbazole moiety serving as an electron donor (D) and an indole group functioning as an electron acceptor (A), thus adopting a classical <em>d</em>-A molecular structure. Utilizing the twisted intramolecular charge transfer, M-KZ-C8 exhibits exceptional sensitivity to viscosity fluctuations. Moreover, we have observed that M-KZ-C8 possesses an impressive capability for precisely monitoring mitochondrial viscosity changes within living cells, unaffected by alterations in MMP. Leveraging M-KZ-C8, we have successfully imaged heightened mitochondrial viscosity in fatty liver and cancerous tissues. Remarkably, the probe has achieved the visualization of mitochondrial viscosity not only at tissue and organ levels but also in surgical specimens from clinical cancer patients for the first time, heralding its vast potential for clinical diagnosis of human cancers. These attributes render M-KZ-C8 an efficacious diagnostic tool for the precise detection, unwavering fidelity, and prolonged imaging of diseases associated with mitochondrial viscosity.</div></div>","PeriodicalId":277,"journal":{"name":"Chinese Journal of Analytical Chemistry","volume":"53 12","pages":"Article 100630"},"PeriodicalIF":1.3,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145465064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A novel electrochemical sensing platform was developed based on copper-based metal-organic framework (Cu-MOF) microcapsules synthesized via a Pickering emulsion templating approach, where carbon nanoparticles used as solid emulsifiers to enhance electrode interfacial interactions and material conductivity. This design synergistically combines the superior catalytic activity of Cu-MOF with the conductive carbon matrix. The Cu-MOF microcapsules were applied in the modification of a glassy carbon electrode to obtain an electrochemical sensor for simultaneous detection of dopamine and paracetamol. This sensor showed the good sensing performance with a broad linearity range (2–1600 μM for dopamine and 2–6500 μM for paracetamol) and low detection limits (1.69 μM for dopamine and 1.81 μM for paracetamol at S/N = 3). This platform also demonstrated excellent reproducibility (RSD < 3 %) and selectivity against common interferents, highlighting its potential for practical applications in real sample detection.
{"title":"A novel electrochemical sensor based on Pickering-droplet-derived Cu-MOF microcapsules for simultaneous determination of dopamine and paracetamol","authors":"Danping TIAN , Ruipeng HAO , Yanzi ZHANG , Wanyu XIE , Pei ZHANG , Wenbo LU","doi":"10.1016/j.cjac.2025.100623","DOIUrl":"10.1016/j.cjac.2025.100623","url":null,"abstract":"<div><div>A novel electrochemical sensing platform was developed based on copper-based metal-organic framework (Cu-MOF) microcapsules synthesized <em>via</em> a Pickering emulsion templating approach, where carbon nanoparticles used as solid emulsifiers to enhance electrode interfacial interactions and material conductivity. This design synergistically combines the superior catalytic activity of Cu-MOF with the conductive carbon matrix. The Cu-MOF microcapsules were applied in the modification of a glassy carbon electrode to obtain an electrochemical sensor for simultaneous detection of dopamine and paracetamol. This sensor showed the good sensing performance with a broad linearity range (2–1600 μM for dopamine and 2–6500 μM for paracetamol) and low detection limits (1.69 μM for dopamine and 1.81 μM for paracetamol at S/<em>N</em> = 3). This platform also demonstrated excellent reproducibility (RSD < 3 %) and selectivity against common interferents, highlighting its potential for practical applications in real sample detection.</div></div>","PeriodicalId":277,"journal":{"name":"Chinese Journal of Analytical Chemistry","volume":"53 11","pages":"Article 100623"},"PeriodicalIF":1.3,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145264944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-08-05DOI: 10.1016/j.cjac.2025.100609
Xiaoshuang ZHOU , Xiaofeng MA , Kai HUANG , Tianyu SHI , Qin TIAN , Tianli ZHANG , Shude YANG , Xianhao CHENG , Rui ZHANG , Yongfei MING
Inonotus hispidus (I. hispidus) is rich in bioactive polysaccharides, making it a valuable dietary supplement and medicinal ingredient. This study employed the three-phase separation method (TPP) to extract and purify polysaccharides from I. hispidus, designated as SH-T. The resulting polysaccharides were then compared with those obtained through the traditional hot water extraction and ethanol precipitation method, designated as SHC, in terms of their physicochemical properties and in vitro antioxidant activity. SH-T extraction achieved maximum efficiency 20 % ammonium sulfate, 1:2 (sample:tert‑butanol) phase ratio, and 25 °C. Under these circumstances, SH-T exhibited a higher protein removal rate, with a yield of 11.10 % and a polysaccharide content of 60.84 %, nearly twice that of SHC obtained through the WEAP method. SH-T and SHC exhibited similar structural characteristics. SH-T displayed superior performance in scavenging DPPH–, ABTS+, and •OH while exhibiting higher reducing power than SHC. In conclusion, TPP demonstrated efficacy in extracting polysaccharides from I. hispidus, yielding products of high purity and enhanced antioxidant properties, thus presenting a promising methodology for the isolation of polysaccharides from I. hispidus.
{"title":"Optimized extraction and antioxidant activity of polysaccharides from Inonotus hispidus using three-phase partitioning","authors":"Xiaoshuang ZHOU , Xiaofeng MA , Kai HUANG , Tianyu SHI , Qin TIAN , Tianli ZHANG , Shude YANG , Xianhao CHENG , Rui ZHANG , Yongfei MING","doi":"10.1016/j.cjac.2025.100609","DOIUrl":"10.1016/j.cjac.2025.100609","url":null,"abstract":"<div><div><em>Inonotus hispidus</em> (<em>I. hispidus</em>) is rich in bioactive polysaccharides, making it a valuable dietary supplement and medicinal ingredient. This study employed the three-phase separation method (TPP) to extract and purify polysaccharides from <em>I. hispidus</em>, designated as SH-T. The resulting polysaccharides were then compared with those obtained through the traditional hot water extraction and ethanol precipitation method, designated as SH<img>C, in terms of their physicochemical properties and <em>in vitro</em> antioxidant activity. SH-T extraction achieved maximum efficiency 20 % ammonium sulfate, 1:2 (sample:tert‑butanol) phase ratio, and 25 °C. Under these circumstances, SH-T exhibited a higher protein removal rate, with a yield of 11.10 % and a polysaccharide content of 60.84 %, nearly twice that of SH<img>C obtained through the WEAP method. SH-T and SH<img>C exhibited similar structural characteristics. SH-T displayed superior performance in scavenging DPPH<sup>–</sup>, ABTS<sup>+</sup>, and •OH while exhibiting higher reducing power than SH<img>C. In conclusion, TPP demonstrated efficacy in extracting polysaccharides from <em>I. hispidus</em>, yielding products of high purity and enhanced antioxidant properties, thus presenting a promising methodology for the isolation of polysaccharides from <em>I. hispidus</em>.</div></div>","PeriodicalId":277,"journal":{"name":"Chinese Journal of Analytical Chemistry","volume":"53 12","pages":"Article 100609"},"PeriodicalIF":1.3,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145527515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-07-22DOI: 10.1016/j.cjac.2025.100600
Zhuo-Fan XIE, Hao-liang ZHANG, Zao-liang MA, Su-Fang CHEN, Jian-Chun MA
Non-alcoholic fatty liver disease (NAFLD), the most prevalent chronic liver disorder worldwide, is characterized by excessive triglyceride accumulation in the liver. It is strongly associated with metabolic syndrome, type 2 diabetes mellitus, obesity, and cardiovascular diseases. Fueled by the global increase in obesity rates, NAFLD has emerged as the most common liver disease globally. In this study, we utilized liquid chromatography-tandem mass spectrometry (LC-MS/MS) to comprehensively characterize the chemical constituents of Millettia speciosa Champ. By combining network pharmacology and molecular docking techniques, we constructed a “component-target-pathway” network to elucidate the therapeutic targets and mechanisms underlying the anti-NAFLD effects of Millettia speciosa Champ. This integrated approach offers novel insights into its pharmacological activity and identifies potential clinical applications for NAFLD management.
{"title":"Multi-omics integration of LC-MS/MS, network pharmacology, and molecular docking reveals pleiotropic mechanisms of Millettia speciosa Champ. in NAFLD therapy","authors":"Zhuo-Fan XIE, Hao-liang ZHANG, Zao-liang MA, Su-Fang CHEN, Jian-Chun MA","doi":"10.1016/j.cjac.2025.100600","DOIUrl":"10.1016/j.cjac.2025.100600","url":null,"abstract":"<div><div>Non-alcoholic fatty liver disease (NAFLD), the most prevalent chronic liver disorder worldwide, is characterized by excessive triglyceride accumulation in the liver. It is strongly associated with metabolic syndrome, type 2 diabetes mellitus, obesity, and cardiovascular diseases. Fueled by the global increase in obesity rates, NAFLD has emerged as the most common liver disease globally. In this study, we utilized liquid chromatography-tandem mass spectrometry (LC-MS/MS) to comprehensively characterize the chemical constituents of <em>Millettia speciosa Champ.</em> By combining network pharmacology and molecular docking techniques, we constructed a “component-target-pathway” network to elucidate the therapeutic targets and mechanisms underlying the anti-NAFLD effects of <em>Millettia speciosa Champ.</em> This integrated approach offers novel insights into its pharmacological activity and identifies potential clinical applications for NAFLD management.</div></div>","PeriodicalId":277,"journal":{"name":"Chinese Journal of Analytical Chemistry","volume":"53 12","pages":"Article 100600"},"PeriodicalIF":1.3,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145527516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-07-14DOI: 10.1016/j.cjac.2025.100589
Pompy Sarkar , Jayanta K. Ray , Atiur Ahmed
An efficient approach has been discovered towards the preparation of 6H-benzo[c]chromenes and analogues via the in situ developed palladium nanoparticle-catalyzed cascade of Suzuki-Miyaura cross-coupling and intramolecular SN2 reaction under aerobic conditions. To make the reactions eco-friendly, water was chosen as the solvent along with the use of phosphine ligand was avoided. The reactions were found to be highly efficient with the productions of the chromenes upto the excellent yield of 96 % at 80 °C. Therefore, the several advantages like eco-friendly, mild, easy to handle, tolerance of various functional groups and efficient production of diverse benzo[c]chromenes have made this method as novel.
{"title":"In situ produced palladium nanoparticle-catalyzed efficient synthesis of benzo[c]chromenes and analogues under aerobic and ligand-free conditions in water","authors":"Pompy Sarkar , Jayanta K. Ray , Atiur Ahmed","doi":"10.1016/j.cjac.2025.100589","DOIUrl":"10.1016/j.cjac.2025.100589","url":null,"abstract":"<div><div>An efficient approach has been discovered towards the preparation of 6<em>H</em>-benzo[<em>c</em>]chromenes and analogues <em>via</em> the <em>in situ</em> developed palladium nanoparticle-catalyzed cascade of Suzuki-Miyaura cross-coupling and intramolecular S<sub>N</sub>2 reaction under aerobic conditions. To make the reactions eco-friendly, water was chosen as the solvent along with the use of phosphine ligand was avoided. The reactions were found to be highly efficient with the productions of the chromenes upto the excellent yield of 96 % at 80 °C. Therefore, the several advantages like eco-friendly, mild, easy to handle, tolerance of various functional groups and efficient production of diverse benzo[<em>c</em>]chromenes have made this method as novel.</div></div>","PeriodicalId":277,"journal":{"name":"Chinese Journal of Analytical Chemistry","volume":"53 10","pages":"Article 100589"},"PeriodicalIF":1.3,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145060098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-07-11DOI: 10.1016/j.cjac.2025.100594
May N. Bin JUMAH , Ahmed M. El-SHERBEENY , Amna M. FARHAN , Ahmed A. ALLAM , Wail Al ZOUBI , Mostafa R. ABUKHADRA
This study explores the potential of spent black tea (S.BT) as a heterogeneous acidic catalyst for biodiesel production from waste cooking oil (WCO) via transesterification. S.BT, a waste material, was sulfonated without carbonization, resulting in a catalyst with enhanced catalytic properties. The catalyst was characterized for key physicochemical properties, including an acid density of 34.6 mmol/g, surface area of 58.3 m2/g, and sulfur content of 3.14 wt%. Under optimal reaction conditions—50 °C, 70 min, 3 wt% S.BT catalyst loading, and a methanol/oil ratio of 10:1—transesterification of WCO with methanol yielded biodiesel with a maximum conversion of 97.3 %. A statistical optimization approach using response surface methodology (RSM) demonstrated that biodiesel yield can be increased to over 99 % by adjusting factors such as temperature, catalyst loading, and methanol/oil ratio. Kinetic analysis revealed that the transesterification followed pseudo-first-order kinetics with an activation energy (Ea) of 34.3 kJ/mol, indicating the reaction proceeds efficiently at moderate temperatures. The thermodynamic analysis, using the Eyring-Polanyi equation, revealed a positive activation enthalpy (ΔH* = 31.76 kJ/mol), indicating the endothermic nature of the process, and a positive entropy (ΔS* = 18.36 J/K·mol), reflecting increased disorder at the transition state. Gibbs free energy (ΔG*) values ranged from –5.35 kJ/mol at 30 °C to –5.71 kJ/mol at 50 °C, confirming the thermodynamic feasibility of the reaction at elevated temperatures. The biodiesel produced met international fuel standards (ASTM D6751 and EN 14214), with a cetane number of 65.3, flash point of 152.3 °C, viscosity of 3.62 mm2/s, and density of 0.837 g/cm3, confirming its suitability for diesel engines. These results underscore the efficiency, sustainability, and cost-effectiveness of S.BT as a catalyst for biodiesel production.
{"title":"Recycling spent black tea as a novel heterogeneous acidic catalyst for efficient biodiesel production from waste cooking oil: Optimization, kinetics, and thermodynamic insights","authors":"May N. Bin JUMAH , Ahmed M. El-SHERBEENY , Amna M. FARHAN , Ahmed A. ALLAM , Wail Al ZOUBI , Mostafa R. ABUKHADRA","doi":"10.1016/j.cjac.2025.100594","DOIUrl":"10.1016/j.cjac.2025.100594","url":null,"abstract":"<div><div>This study explores the potential of spent black tea (S.BT) as a heterogeneous acidic catalyst for biodiesel production from waste cooking oil (WCO) via transesterification. S.BT, a waste material, was sulfonated without carbonization, resulting in a catalyst with enhanced catalytic properties. The catalyst was characterized for key physicochemical properties, including an acid density of 34.6 mmol/g, surface area of 58.3 m<sup>2</sup>/g, and sulfur content of 3.14 wt%. Under optimal reaction conditions—50 °C, 70 min, 3 wt% S.BT catalyst loading, and a methanol/oil ratio of 10:1—transesterification of WCO with methanol yielded biodiesel with a maximum conversion of 97.3 %. A statistical optimization approach using response surface methodology (RSM) demonstrated that biodiesel yield can be increased to over 99 % by adjusting factors such as temperature, catalyst loading, and methanol/oil ratio. Kinetic analysis revealed that the transesterification followed pseudo-first-order kinetics with an activation energy (<em>E</em><sub>a</sub>) of 34.3 kJ/mol, indicating the reaction proceeds efficiently at moderate temperatures. The thermodynamic analysis, using the Eyring-Polanyi equation, revealed a positive activation enthalpy (Δ<em>H</em>* = 31.76 kJ/mol), indicating the endothermic nature of the process, and a positive entropy (Δ<em>S</em>* = 18.36 J/K·mol), reflecting increased disorder at the transition state. Gibbs free energy (Δ<em>G</em>*) values ranged from –5.35 kJ/mol at 30 °C to –5.71 kJ/mol at 50 °C, confirming the thermodynamic feasibility of the reaction at elevated temperatures. The biodiesel produced met international fuel standards (ASTM <span><span>D6751</span><svg><path></path></svg></span> and EN 14214), with a cetane number of 65.3, flash point of 152.3 °C, viscosity of 3.62 mm<sup>2</sup>/s, and density of 0.837 g/cm<sup>3</sup>, confirming its suitability for diesel engines. These results underscore the efficiency, sustainability, and cost-effectiveness of S.BT as a catalyst for biodiesel production.</div></div>","PeriodicalId":277,"journal":{"name":"Chinese Journal of Analytical Chemistry","volume":"53 11","pages":"Article 100594"},"PeriodicalIF":1.3,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145334703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-07-09DOI: 10.1016/j.cjac.2025.100595
Mengxia YANG , Shengbao HE , Fei YANG , Huimin DENG , Yanbo ZENG , Chunqiong WANG , Fengqin CHANG , Guangzhi HU
Lead (Pb) in the environment are a source of environmental pollution and also presents serious risks to public health. Accordingly, developing rapid and effective Pb(II) detection techniques is essential for environmental protection and biosafety. For Pb(II) detection, tubular Fe2O3-graphitic carbon nitride (GCN) composites were successfully prepared herein, and the morphology, structure, and chemical composition were characterized by utilizing various characterization techniques. Fe2O3-GCN was prepared by modifying GCN with Fe2O3, and an Fe2O3-GCNNafion/GCE electrochemical sensor was successfully developed. Under the optimal conditions, the electrochemical signals showed linear correlation with the Pb(II) concentration across two ranges, 3‒15 μg/L and 15‒80 μg/L, and achieving a low detection limit (0.084 μg/L). The Fe2O3-GCNNafion/GCE electrochemical sensor exhibited excellent stability, resistance to interference, and good applicability in detecting Pb(II) in both surface water and tobacco digestion solution. This study provides a new strategy for heavy-metal sensing in environmental and tobacco samples, while simultaneously expanding the application of GCN composites in sensing platforms.
{"title":"Fe2O3-functionalized graphite carbon nitride microtube-based electrochemical sensor for sensitive quantification of lead in tobacco and environmental samples","authors":"Mengxia YANG , Shengbao HE , Fei YANG , Huimin DENG , Yanbo ZENG , Chunqiong WANG , Fengqin CHANG , Guangzhi HU","doi":"10.1016/j.cjac.2025.100595","DOIUrl":"10.1016/j.cjac.2025.100595","url":null,"abstract":"<div><div>Lead (Pb) in the environment are a source of environmental pollution and also presents serious risks to public health. Accordingly, developing rapid and effective Pb(II) detection techniques is essential for environmental protection and biosafety. For Pb(II) detection, tubular Fe<sub>2</sub>O<sub>3</sub>-graphitic carbon nitride (GCN) composites were successfully prepared herein, and the morphology, structure, and chemical composition were characterized by utilizing various characterization techniques. Fe<sub>2</sub>O<sub>3</sub>-GCN was prepared by modifying GCN with Fe<sub>2</sub>O<sub>3,</sub> and an Fe<sub>2</sub>O<sub>3</sub>-GCN<img>Nafion/GCE electrochemical sensor was successfully developed. Under the optimal conditions, the electrochemical signals showed linear correlation with the Pb(II) concentration across two ranges, 3‒15 μg/L and 15‒80 μg/L, and achieving a low detection limit (0.084 μg/L). The Fe<sub>2</sub>O<sub>3</sub>-GCN<img>Nafion/GCE electrochemical sensor exhibited excellent stability, resistance to interference, and good applicability in detecting Pb(II) in both surface water and tobacco digestion solution. This study provides a new strategy for heavy-metal sensing in environmental and tobacco samples, while simultaneously expanding the application of GCN composites in sensing platforms.</div></div>","PeriodicalId":277,"journal":{"name":"Chinese Journal of Analytical Chemistry","volume":"53 11","pages":"Article 100595"},"PeriodicalIF":1.3,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145334700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
扫码关注我们
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号