Pub Date : 2026-04-01Epub Date: 2025-12-30DOI: 10.1016/j.colsurfb.2025.115393
Yunna Xue, Xiangyang Zhang, Xinggui Zhou
Chiral drugs often exhibit stereoselective pharmacological activity, yet their separation remains challenging due to virtually identical physicochemical properties. Here, we propose a tunable additive strategy using agarose hydrogels (AG) as chiral enantioseparation scaffolds, where non-covalent interactions between drug and additives enable chiral recognition. Using racemic ibuprofen (Ibu) as a model compound, Fourier transform infrared spectroscopy, scanning electron microscopy, and X-ray diffraction analyses revealed the enhanced loading, modified morphology, and altered crystallinity of hydrophobic Ibu induced by additives. Non-covalent interactions between the additives and Ibu, revealed by molecular docking, quantum chemical calculation, and IRI analysis, promote the dispersion of Ibu within the matrix and preferentially release the pharmacologically active enantiomer. Notably, while additive free AG afforded an enantiomeric excess of 1.7 %, the cyclodextrin-modified hydrogel achieved up to 15.6 %, a more than 9 times improvement, highlighting the potential of this strategy for the adjustable enantioseparation at the biointerface.
{"title":"A tunable additive strategy for enantioseparation of hydrophobic racemic ibuprofen through agarose-based hydrogels","authors":"Yunna Xue, Xiangyang Zhang, Xinggui Zhou","doi":"10.1016/j.colsurfb.2025.115393","DOIUrl":"10.1016/j.colsurfb.2025.115393","url":null,"abstract":"<div><div>Chiral drugs often exhibit stereoselective pharmacological activity, yet their separation remains challenging due to virtually identical physicochemical properties. Here, we propose a tunable additive strategy using agarose hydrogels (AG) as chiral enantioseparation scaffolds, where non-covalent interactions between drug and additives enable chiral recognition. Using racemic ibuprofen (Ibu) as a model compound, Fourier transform infrared spectroscopy, scanning electron microscopy, and X-ray diffraction analyses revealed the enhanced loading, modified morphology, and altered crystallinity of hydrophobic Ibu induced by additives. Non-covalent interactions between the additives and Ibu, revealed by molecular docking, quantum chemical calculation, and IRI analysis, promote the dispersion of Ibu within the matrix and preferentially release the pharmacologically active enantiomer. Notably, while additive free AG afforded an enantiomeric excess of 1.7 %, the cyclodextrin-modified hydrogel achieved up to 15.6 %, a more than 9 times improvement, highlighting the potential of this strategy for the adjustable enantioseparation at the biointerface.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"260 ","pages":"Article 115393"},"PeriodicalIF":5.6,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145916340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-04-01Epub Date: 2025-12-17DOI: 10.1016/j.colsurfb.2025.115382
Hao Shen , Tong Li , Xueying Chen , Zhurun Fang , Yan Xu , Kai Zheng , Ming Zhang
As the primary physical and immunological barrier against external insults, skin integrity is frequently compromised by trauma, disease, or iatrogenic injury, leading to pathogenic microorganism invasion and infected wounds. The escalating challenges posed by bacterial antibiotic resistance and biofilm formation highlight the urgent need for therapeutic systems that synergistically combine potent antibacterial efficacy with effective tissue regeneration modulation. Therefore, this study developed a multifunctional hydrogel platform integrating rapid photothermal sterilization and pro-regenerative bioactivity. We engineered an aggregation-induced emission (AIE) nanoparticle-loaded type I recombinant humanized collagen hydrogel (AIE@RHCI), achieving rapid UV-triggered crosslinking from liquid to gel state. The hydrogel demonstrates appropriate swelling capacity, controllable degradation kinetics, and favorable mechanical properties, enabling stable adhesion to dynamically deforming skin during movement. The incorporated AIE component exhibits 39.7 % photothermal conversion efficiency under 808 nm near-infrared (NIR) irradiation. In vitro studies confirmed that the AIE@RHCI hydrogel under NIR irradiation reduced methicillin-resistant Staphylococcus aureus (MRSA) viability to 22.2 %, while concurrently achieving 79.8 % biofilm inhibition. Simultaneously, the recombinant collagen matrix promotes vascular endothelial cell migration and accelerates vascularization, with significant upregulation of key pro-angiogenic genes VEGF and CD31. This integrated platform synergistically combines photothermal bactericidal nanoparticles with bioactive recombinant collagen scaffolding, offering a novel and promising therapeutic strategy for the management of antibiotic-resistant wound infections, with potential for clinical translation in chronic and infected wound care.
{"title":"Multifunctional collagen hydrogel accelerates infected wound repair through photothermal disinfection and pro-angiogenic activity","authors":"Hao Shen , Tong Li , Xueying Chen , Zhurun Fang , Yan Xu , Kai Zheng , Ming Zhang","doi":"10.1016/j.colsurfb.2025.115382","DOIUrl":"10.1016/j.colsurfb.2025.115382","url":null,"abstract":"<div><div>As the primary physical and immunological barrier against external insults, skin integrity is frequently compromised by trauma, disease, or iatrogenic injury, leading to pathogenic microorganism invasion and infected wounds. The escalating challenges posed by bacterial antibiotic resistance and biofilm formation highlight the urgent need for therapeutic systems that synergistically combine potent antibacterial efficacy with effective tissue regeneration modulation. Therefore, this study developed a multifunctional hydrogel platform integrating rapid photothermal sterilization and pro-regenerative bioactivity. We engineered an aggregation-induced emission (AIE) nanoparticle-loaded type I recombinant humanized collagen hydrogel (AIE@RHCI), achieving rapid UV-triggered crosslinking from liquid to gel state. The hydrogel demonstrates appropriate swelling capacity, controllable degradation kinetics, and favorable mechanical properties, enabling stable adhesion to dynamically deforming skin during movement. The incorporated AIE component exhibits 39.7 % photothermal conversion efficiency under 808 nm near-infrared (NIR) irradiation. <em>In vitro</em> studies confirmed that the AIE@RHCI hydrogel under NIR irradiation reduced methicillin-resistant <em>Staphylococcus aureus</em> (MRSA) viability to 22.2 %, while concurrently achieving 79.8 % biofilm inhibition. Simultaneously, the recombinant collagen matrix promotes vascular endothelial cell migration and accelerates vascularization, with significant upregulation of key pro-angiogenic genes <em>VEGF</em> and <em>CD31</em>. This integrated platform synergistically combines photothermal bactericidal nanoparticles with bioactive recombinant collagen scaffolding, offering a novel and promising therapeutic strategy for the management of antibiotic-resistant wound infections, with potential for clinical translation in chronic and infected wound care.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"260 ","pages":"Article 115382"},"PeriodicalIF":5.6,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145792787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-04-01Epub Date: 2025-12-31DOI: 10.1016/j.colsurfb.2025.115407
Jan Kobierski , Sławomir Stachura , Anita Wnętrzak , Anna Chachaj-Brekiesz , Michał Świątek , Wojciech Jawień , Patrycja Dynarowicz-Latka
Water is a critical determinant of interfacial behavior, therefore the choice of water model in molecular dynamics (MD) simulations can profoundly influence the accuracy of predicted monolayer properties. In this work, we compare the impact of two water models—three-point TIP3P and four-point OPC—on the interfacial organization, hydration, and hydrogen bonding of epimeric 22-hydroxycholesterols [22(R)-OH and 22(S)-OH] in model membranes. All-atom MD simulations, complemented by Langmuir monolayer experiments, demonstrate that the water model selection strongly affects monolayer behavior: the OPC model produces sharper density gradients and reduced water penetration at the interface, whereas TIP3P permits deeper water ingress and yields a more diffuse interfacial region. As a result, stereochemical differences are more pronounced with the OPC model, particularly for the hydration-prone 22(R)-OH. Specifically, 22(S)-OH forms more condensed monolayers with the side-chain hydroxyl group buried within the interface, whereas 22(R)-OH generates more expanded monolayers characterized by enhanced hydration in close agreement with experimental observations. These stereochemistry-dependent effects arise from distinct hydrogen-bonding patterns: 22(S)-OH preferentially engages in sterol–sterol hydrogen bonds, while 22(R)-OH remains more hydrated through hydrogen bonding with water molecules. Collectively, these findings underscore the importance of accurate water model selection for reliable representation of biointerface properties and highlight that even subtle stereochemical modifications can exert disproportionate effects on interfacial organization and hydration.
{"title":"How water models influence the interfacial organization of oxysterol epimers: A comparative simulation study using TIP3P and OPC","authors":"Jan Kobierski , Sławomir Stachura , Anita Wnętrzak , Anna Chachaj-Brekiesz , Michał Świątek , Wojciech Jawień , Patrycja Dynarowicz-Latka","doi":"10.1016/j.colsurfb.2025.115407","DOIUrl":"10.1016/j.colsurfb.2025.115407","url":null,"abstract":"<div><div>Water is a critical determinant of interfacial behavior, therefore the choice of water model in molecular dynamics (MD) simulations can profoundly influence the accuracy of predicted monolayer properties. In this work, we compare the impact of two water models—three-point TIP3P and four-point OPC—on the interfacial organization, hydration, and hydrogen bonding of epimeric 22-hydroxycholesterols [22(<em>R</em>)-OH and 22(<em>S</em>)-OH] in model membranes. All-atom MD simulations, complemented by Langmuir monolayer experiments, demonstrate that the water model selection strongly affects monolayer behavior: the OPC model produces sharper density gradients and reduced water penetration at the interface, whereas TIP3P permits deeper water ingress and yields a more diffuse interfacial region. As a result, stereochemical differences are more pronounced with the OPC model, particularly for the hydration-prone 22(<em>R</em>)-OH. Specifically, 22(<em>S</em>)-OH forms more condensed monolayers with the side-chain hydroxyl group buried within the interface, whereas 22(<em>R</em>)-OH generates more expanded monolayers characterized by enhanced hydration in close agreement with experimental observations. These stereochemistry-dependent effects arise from distinct hydrogen-bonding patterns: 22(<em>S</em>)-OH preferentially engages in sterol–sterol hydrogen bonds, while 22(<em>R</em>)-OH remains more hydrated through hydrogen bonding with water molecules. Collectively, these findings underscore the importance of accurate water model selection for reliable representation of biointerface properties and highlight that even subtle stereochemical modifications can exert disproportionate effects on interfacial organization and hydration.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"260 ","pages":"Article 115407"},"PeriodicalIF":5.6,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145881523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-04-01Epub Date: 2025-12-18DOI: 10.1016/j.colsurfb.2025.115385
Isabella Walker, Huiyan Li
Early detection of diseases significantly improves patient outcomes, yet many biomarkers remain at ultra-low concentrations in body fluids during the initial stages, posing challenges for conventional diagnostic techniques. Extracellular vesicles (EVs) have emerged as promising non-invasive biomarkers due to their presence in body fluids such as blood, saliva and urine, and because their molecular cargo reflects their cells of origin. However, sensitive and accurate detection of disease related EVs is challenging because of their low concentrations and nanoscale size. Metal enhanced fluorescence (MEF) has recently been applied to overcome these limitations by amplifying the fluorescent signal of labeled EVs through localized surface plasmon resonances of metallic nanostructures. This amplification enables the detection and quantification of EV-associated biomarkers with improved sensitivity, offering potential for earlier disease diagnosis. However, to date, few reviews have focused specifically on MEF-based applications for the detection and quantification of EVs and their molecular cargo. This review examines recent advances in MEF-based platforms for EV analysis, including nanohole arrays, metal nano islands and nanoarrays, gold nanoparticles in three-dimensional matrices, metal-organic frameworks and other plasmonic approaches. While current reviews have discussed the use of MEF biosensing application, this article specifically focuses on MEF- based EV detection highlighting the design principles, optical properties, advantages, and limitations of these platforms. Collectively, MEF offers a powerful strategy to enhance EV detection, bridging the gap between experimental analysis and clinical application.
{"title":"Applications of metal enhanced fluorescence for the detection and quantification of extracellular vesicles","authors":"Isabella Walker, Huiyan Li","doi":"10.1016/j.colsurfb.2025.115385","DOIUrl":"10.1016/j.colsurfb.2025.115385","url":null,"abstract":"<div><div>Early detection of diseases significantly improves patient outcomes, yet many biomarkers remain at ultra-low concentrations in body fluids during the initial stages, posing challenges for conventional diagnostic techniques. Extracellular vesicles (EVs) have emerged as promising non-invasive biomarkers due to their presence in body fluids such as blood, saliva and urine, and because their molecular cargo reflects their cells of origin. However, sensitive and accurate detection of disease related EVs is challenging because of their low concentrations and nanoscale size. Metal enhanced fluorescence (MEF) has recently been applied to overcome these limitations by amplifying the fluorescent signal of labeled EVs through localized surface plasmon resonances of metallic nanostructures. This amplification enables the detection and quantification of EV-associated biomarkers with improved sensitivity, offering potential for earlier disease diagnosis. However, to date, few reviews have focused specifically on MEF-based applications for the detection and quantification of EVs and their molecular cargo. This review examines recent advances in MEF-based platforms for EV analysis, including nanohole arrays, metal nano islands and nanoarrays, gold nanoparticles in three-dimensional matrices, metal-organic frameworks and other plasmonic approaches. While current reviews have discussed the use of MEF biosensing application, this article specifically focuses on MEF- based EV detection highlighting the design principles, optical properties, advantages, and limitations of these platforms. Collectively, MEF offers a powerful strategy to enhance EV detection, bridging the gap between experimental analysis and clinical application.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"260 ","pages":"Article 115385"},"PeriodicalIF":5.6,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145799421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-04-01Epub Date: 2025-12-08DOI: 10.1016/j.colsurfb.2025.115356
Li Li , Junxiong Wen , Hao Xu , Hongyuan Yu , Xue Bai , Yue Zhang
Pancreatic ductal adenocarcinoma (PDAC) remains one of the most treatment-resistant malignancies, primarily attributed to its dense and fibrotic extracellular matrix (ECM), which impedes both immune cell infiltration and drug delivery. Despite the remarkable success of immunotherapy in other cancer types, its efficacy in PDAC has been significantly compromised by the immune-exclusion barrier. Recent advancements in nanomedicine offer a compelling strategy to overcome this hurdle through the development of ECM-responsive delivery systems. These platforms are engineered to respond to ECM-specific triggers such as hyaluronidase or matrix metalloproteinases, enabling localized degradation of the tumor stroma while simultaneously releasing immunostimulatory agents. This review summarizes the unique ECM biology of PDAC, current limitations of immunotherapy, and recent breakthroughs in stimuli-responsive nanotechnology. This study also comprehensively discusses design principles, preclinical validation outcomes, translational challenges, and emerging strategies integrating ECM modulation with next-generation immune activation. ECM-responsive nanomedicine represents a transformative paradigm in stroma-immune co-engineering and offers a promising avenue for unlocking immunotherapy in PDAC.
{"title":"ECM-responsive nanomedicine to enhance immunotherapy in pancreatic cancer","authors":"Li Li , Junxiong Wen , Hao Xu , Hongyuan Yu , Xue Bai , Yue Zhang","doi":"10.1016/j.colsurfb.2025.115356","DOIUrl":"10.1016/j.colsurfb.2025.115356","url":null,"abstract":"<div><div>Pancreatic ductal adenocarcinoma (PDAC) remains one of the most treatment-resistant malignancies, primarily attributed to its dense and fibrotic extracellular matrix (ECM), which impedes both immune cell infiltration and drug delivery. Despite the remarkable success of immunotherapy in other cancer types, its efficacy in PDAC has been significantly compromised by the immune-exclusion barrier. Recent advancements in nanomedicine offer a compelling strategy to overcome this hurdle through the development of ECM-responsive delivery systems. These platforms are engineered to respond to ECM-specific triggers such as hyaluronidase or matrix metalloproteinases, enabling localized degradation of the tumor stroma while simultaneously releasing immunostimulatory agents. This review summarizes the unique ECM biology of PDAC, current limitations of immunotherapy, and recent breakthroughs in stimuli-responsive nanotechnology. This study also comprehensively discusses design principles, preclinical validation outcomes, translational challenges, and emerging strategies integrating ECM modulation with next-generation immune activation. ECM-responsive nanomedicine represents a transformative paradigm in stroma-immune co-engineering and offers a promising avenue for unlocking immunotherapy in PDAC.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"260 ","pages":"Article 115356"},"PeriodicalIF":5.6,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145799422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hydrogels are promising materials for minimally invasive surgical interventions and tissue regeneration. However, hydrogels derived from the extracellular matrix (ECM) frequently suffer from rapid degradation, poor injectability, limited bioactivity, and weak stability. The purpose of this work is to combine decellularized placental extracellular matrix (PECM) hydrogels with tannic acid (TA) to enhance the stability and biological characteristics. PECM hydrogels were subjected to proteomic analysis to detect and describe bioactive proteins. PECM hydrogels crosslinked with TA demonstrated antibacterial efficiency, antioxidant activity, hemocompatibility, and biocompatibility. Notable improvements in stability were observed following enzymatic degradation and rheological characterization. Mice treated with PECM hydrogels crosslinked with TA in a diabetic wound healing model show sustained vascularization, reduced inflammation over time, faster wound closure, and cell infiltration. These findings highlight the synergistic effect of TA crosslinking in modulating both the structural integrity and the biological function of PECM hydrogels. Furthermore, the integration of TA contributes to the controlled degradation profile, enabling prolonged residence time at the wound site for effective tissue regeneration. The enhanced stability, presence of bioactive molecules, and microenvironment for host remodelling, as well as the demonstrated therapeutic efficacy of these hydrogels, suggest significant promise for their clinical application in tissue repair.
{"title":"Bioactive human placental ECM hydrogels crosslinked with tannic acid enhance stability and antioxidant properties for diabetic wound healing","authors":"Sunil Gujjar , Pratibha Jaipal , Prakash Jayabal , Bhisma Narayan Panda , Priyanka Sharma , Jagadish Chandra Sharma , Anil Kumar Pandey , Santosh Mathapati","doi":"10.1016/j.colsurfb.2025.115406","DOIUrl":"10.1016/j.colsurfb.2025.115406","url":null,"abstract":"<div><div>Hydrogels are promising materials for minimally invasive surgical interventions and tissue regeneration. However, hydrogels derived from the extracellular matrix (ECM) frequently suffer from rapid degradation, poor injectability, limited bioactivity, and weak stability. The purpose of this work is to combine decellularized placental extracellular matrix (PECM) hydrogels with tannic acid (TA) to enhance the stability and biological characteristics. PECM hydrogels were subjected to proteomic analysis to detect and describe bioactive proteins. PECM hydrogels crosslinked with TA demonstrated antibacterial efficiency, antioxidant activity, hemocompatibility, and biocompatibility. Notable improvements in stability were observed following enzymatic degradation and rheological characterization. Mice treated with PECM hydrogels crosslinked with TA in a diabetic wound healing model show sustained vascularization, reduced inflammation over time, faster wound closure, and cell infiltration. These findings highlight the synergistic effect of TA crosslinking in modulating both the structural integrity and the biological function of PECM hydrogels. Furthermore, the integration of TA contributes to the controlled degradation profile, enabling prolonged residence time at the wound site for effective tissue regeneration. The enhanced stability, presence of bioactive molecules, and microenvironment for host remodelling, as well as the demonstrated therapeutic efficacy of these hydrogels, suggest significant promise for their clinical application in tissue repair.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"260 ","pages":"Article 115406"},"PeriodicalIF":5.6,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145881607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-04-01Epub Date: 2025-12-22DOI: 10.1016/j.colsurfb.2025.115389
Feng Lin , Hong Huang , Jie Long , Renchuan Liang , Yunxi Huang , Xiaoling Luo
Cancer remains a major global health burden due to its high rates of recurrence and resistance to conventional therapies. Although PD-L1/PD-1 immune checkpoint inhibitors (ICIs) have emerged as promising treatments by restoring T cell function and enhancing anti-tumor immunity, their efficacy is often limited by immunosuppressive tumor microenvironment (ITM) and metabolic adaptations that impair T cell activity. To address these challenges, we developed a novel intelligent drug delivery platform that integrates PANoptosis induction, metabolic modulation and targeted nanotherapy. Specifically, hollow mesoporous manganese dioxide (H-MnO2) nanoshells were synthesized and co-loaded with SOAT1 inhibitor avasimibe (Ava). Trop2-specific targeting ligands were further conjugated to enable precise tumor localization, resulting in the multifunctional nanoplatform Ava@HM/Trop2. Upon tumor accumulation via Trop2-mediated targeting, acidic pH conditions and tumor microenvironment (TME) stimuli trigger rapid degradation of the H-MnO2 nanoshells, releasing Ava and inducing PANoptosis. This process activates the cGAS-STING pathway, remodels immunosuppressive TME, mitigates lipid-induced T cell senescence, and synergizes with PD-1 blockade to potentiate anti-tumor immunity. Both in vitro and in vivo experiments demonstrate that Ava@HM/Trop2 achieves efficient tumor targeting, robust tumor cell apoptosis, and improved therapeutic outcomes. This innovative multi-modal strategy highlights a promising avenue for overcoming immunotherapy resistance and advancing the clinical management of solid tumors.
{"title":"A multifunctional nanozyme integrating panoptosis induction and T-cell metabolic reprogramming to augment the efficacy of PD-1 inhibitors","authors":"Feng Lin , Hong Huang , Jie Long , Renchuan Liang , Yunxi Huang , Xiaoling Luo","doi":"10.1016/j.colsurfb.2025.115389","DOIUrl":"10.1016/j.colsurfb.2025.115389","url":null,"abstract":"<div><div>Cancer remains a major global health burden due to its high rates of recurrence and resistance to conventional therapies. Although PD-L1/PD-1 immune checkpoint inhibitors (ICIs) have emerged as promising treatments by restoring T cell function and enhancing anti-tumor immunity, their efficacy is often limited by immunosuppressive tumor microenvironment (ITM) and metabolic adaptations that impair T cell activity. To address these challenges, we developed a novel intelligent drug delivery platform that integrates PANoptosis induction, metabolic modulation and targeted nanotherapy. Specifically, hollow mesoporous manganese dioxide (H-MnO<sub>2</sub>) nanoshells were synthesized and co-loaded with SOAT1 inhibitor avasimibe (Ava). Trop2-specific targeting ligands were further conjugated to enable precise tumor localization, resulting in the multifunctional nanoplatform Ava@HM/Trop2. Upon tumor accumulation via Trop2-mediated targeting, acidic pH conditions and tumor microenvironment (TME) stimuli trigger rapid degradation of the H-MnO<sub>2</sub> nanoshells, releasing Ava and inducing PANoptosis. This process activates the cGAS-STING pathway, remodels immunosuppressive TME, mitigates lipid-induced T cell senescence, and synergizes with PD-1 blockade to potentiate anti-tumor immunity. Both <em>in vitro</em> and <em>in vivo</em> experiments demonstrate that Ava@HM/Trop2 achieves efficient tumor targeting, robust tumor cell apoptosis, and improved therapeutic outcomes. This innovative multi-modal strategy highlights a promising avenue for overcoming immunotherapy resistance and advancing the clinical management of solid tumors.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"260 ","pages":"Article 115389"},"PeriodicalIF":5.6,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145881522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-04-01Epub Date: 2025-12-30DOI: 10.1016/j.colsurfb.2025.115411
Su Jeong Lee , Won Hur , Chang Hyeon Ha , Da Yeong Choi , Jayachandran Venkatesan , Gi Hun Seong
Nanosheets of molybdenum diselenide (MoSe2), a representative two-dimensional (2D) transition metal dichalcogenide, have attracted considerable attention for biological and medical applications due to their excellent biocompatibility and near-infrared (NIR) absorption capabilities. In this work, we present a simple method for preparing 2D therapeutic nanosheets that incorporate glycyrrhizic acid (GA), a naturally derived amphiphilic surfactant with anticancer properties, for the liquid exfoliation of bulk MoSe2 to produce 2D GA-MoSe2 nanosheets. GA is an excellent exfoliation agent because it can achieve a high yield of around 22 %, and it allows for liquid exfoliation of nanosheets in aqueous solutions. To improve their biocompatibility and cellular uptake, we coated them with polyethylene glycol (PEG), resulting in PEG-GA-MoSe2 (PGM). PGM exhibited robust photothermal performance under 808 nm NIR laser irradiation (0.8 W/cm2, 5 min) and achieved a temperature rise of approximately 55 ℃, even at concentrations as low as 30 μg/mL. As a result, the PEG coating did not affect the photothermal performance of PGM and showed cellular uptake results of PGM that increased by around 3.5 times compared to those of the GA-MoSe2 nanosheets. Furthermore, the nanosheets displayed remarkable colloidal stability without indications of structural degradation after multiple laser irradiation cycles. Both in vitro and in vivo experiments demonstrated minimal cytotoxicity, excellent cellular uptake, and prominent photothermal therapeutic effects, resulting in significant tumor reduction or complete elimination in laser-treated tumor regions. These findings establish PGM as a promising biocompatible platform for photothermal cancer therapy that offers efficient NIR-induced hyperthermia for targeted tumor treatment.
{"title":"Biocompatible semiconducting molybdenum diselenide nanosheets with glycyrrhizic acid for enhanced photothermal cancer therapy","authors":"Su Jeong Lee , Won Hur , Chang Hyeon Ha , Da Yeong Choi , Jayachandran Venkatesan , Gi Hun Seong","doi":"10.1016/j.colsurfb.2025.115411","DOIUrl":"10.1016/j.colsurfb.2025.115411","url":null,"abstract":"<div><div>Nanosheets of molybdenum diselenide (MoSe<sub>2</sub>), a representative two-dimensional (2D) transition metal dichalcogenide, have attracted considerable attention for biological and medical applications due to their excellent biocompatibility and near-infrared (NIR) absorption capabilities. In this work, we present a simple method for preparing 2D therapeutic nanosheets that incorporate glycyrrhizic acid (GA), a naturally derived amphiphilic surfactant with anticancer properties, for the liquid exfoliation of bulk MoSe<sub>2</sub> to produce 2D GA-MoSe<sub>2</sub> nanosheets. GA is an excellent exfoliation agent because it can achieve a high yield of around 22 %, and it allows for liquid exfoliation of nanosheets in aqueous solutions. To improve their biocompatibility and cellular uptake, we coated them with polyethylene glycol (PEG), resulting in PEG-GA-MoSe<sub>2</sub> (PGM). PGM exhibited robust photothermal performance under 808 nm NIR laser irradiation (0.8 W/cm<sup>2</sup>, 5 min) and achieved a temperature rise of approximately 55 ℃, even at concentrations as low as 30 μg/mL. As a result, the PEG coating did not affect the photothermal performance of PGM and showed cellular uptake results of PGM that increased by around 3.5 times compared to those of the GA-MoSe<sub>2</sub> nanosheets. Furthermore, the nanosheets displayed remarkable colloidal stability without indications of structural degradation after multiple laser irradiation cycles. Both <em>in vitro</em> and <em>in vivo</em> experiments demonstrated minimal cytotoxicity, excellent cellular uptake, and prominent photothermal therapeutic effects, resulting in significant tumor reduction or complete elimination in laser-treated tumor regions. These findings establish PGM as a promising biocompatible platform for photothermal cancer therapy that offers efficient NIR-induced hyperthermia for targeted tumor treatment.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"260 ","pages":"Article 115411"},"PeriodicalIF":5.6,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145881525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-04-01Epub Date: 2026-01-06DOI: 10.1016/j.colsurfb.2026.115425
Siyuan Liu , Xialing Tu , Jiaxuan Huang , Nianjie Wu , Bin Xia , Guobao Chen
Native tissue interfaces exhibit continuous gradients in mechanical stiffness and extracellular matrix (ECM) composition, which are essential for effective load transmission and functional integration between adjacent tissues. However, accurately replicating these intricate biomechanical gradients in engineered biomaterials remains a significant challenge in interface tissue engineering. In this study, we present a stiffness-gradient methacrylated silk fibroin (SilMA) hydrogel designed to promote tissue interface regeneration, with a focus on mechanical cues as a critical design parameter. Silk fibroin was chemically modified into SilMA, and its concentration systematically varied to produce multilayer hydrogels exhibiting a continuous stiffness gradient ranging from 7.07 ± 4.02 kPa to 71.30 ± 0.97 kPa, effectively mimicking the native mechanical heterogeneity found at tissue interfaces. Functional assays revealed that the low-stiffness layer significantly enhanced angiogenesis, the intermediate-stiffness layer provided an optimal mechanical environment for stem cell osteogenic differentiation, and the high-stiffness layer recapitulated the biomechanical properties of native cartilage tissue. This biomimetic stiffness-gradient SilMA hydrogel scaffold offers a promising strategy for regenerating complex tissue interfaces by harnessing the instructive role of mechanical microenvironments. Our findings underscore the importance of spatially graded mechanical properties in guiding cellular responses and tissue repair, and advance the design of next-generation materials for interface tissue engineering.
{"title":"Fabrication of SilMA hydrogels with stiffness gradients for soft-to-hard interface tissue engineering","authors":"Siyuan Liu , Xialing Tu , Jiaxuan Huang , Nianjie Wu , Bin Xia , Guobao Chen","doi":"10.1016/j.colsurfb.2026.115425","DOIUrl":"10.1016/j.colsurfb.2026.115425","url":null,"abstract":"<div><div>Native tissue interfaces exhibit continuous gradients in mechanical stiffness and extracellular matrix (ECM) composition, which are essential for effective load transmission and functional integration between adjacent tissues. However, accurately replicating these intricate biomechanical gradients in engineered biomaterials remains a significant challenge in interface tissue engineering. In this study, we present a stiffness-gradient methacrylated silk fibroin (SilMA) hydrogel designed to promote tissue interface regeneration, with a focus on mechanical cues as a critical design parameter. Silk fibroin was chemically modified into SilMA, and its concentration systematically varied to produce multilayer hydrogels exhibiting a continuous stiffness gradient ranging from 7.07 ± 4.02 kPa to 71.30 ± 0.97 kPa, effectively mimicking the native mechanical heterogeneity found at tissue interfaces. Functional assays revealed that the low-stiffness layer significantly enhanced angiogenesis, the intermediate-stiffness layer provided an optimal mechanical environment for stem cell osteogenic differentiation, and the high-stiffness layer recapitulated the biomechanical properties of native cartilage tissue. This biomimetic stiffness-gradient SilMA hydrogel scaffold offers a promising strategy for regenerating complex tissue interfaces by harnessing the instructive role of mechanical microenvironments. Our findings underscore the importance of spatially graded mechanical properties in guiding cellular responses and tissue repair, and advance the design of next-generation materials for interface tissue engineering.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"260 ","pages":"Article 115425"},"PeriodicalIF":5.6,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145931700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-04-01Epub Date: 2025-12-23DOI: 10.1016/j.colsurfb.2025.115349
Haiyue Ren , Zhicheng Pan , Jinfeng Yuan , Jing Liu
Glutaraldehyde cross-linked bovine/porcine pericardium has been used as prosthetic heart valves (BHVs), while residual aldehyde groups induce cytotoxicity, thrombus formation, and progressive calcification deposition, which ultimately limits instrument life span to 10–15 years. We innovatively designed a multifunctional zwitterionic hydrogel coating copolymer poly (SBMA-co-DMEMAm) on swim bladder-derived material, which is composed of hydrophilic monomer SBMA and aldehyde-containing monomer DMEMAm; besides the aldehyde group can crosslink fish bladder tissue and improve the mechanical properties. This multifunctional hydrophilic polymer coatings provide effective anti-fouling properties, better anti-platelet adsorption and anti-thrombotic properties in in vitro blood compatibility evaluation. In addition, it showed better anti-inflammatory and anti-calcification properties in rat subcutaneous implantation. Thus, this study presents a versatile zwitterionic hydrogel coating strategy that simultaneously endows decellularized swim bladder with superior anti-fouling, hemocompatibility, cytocompatibility, and anti-thrombogenicity, offering a comprehensive solution for durable bioprosthetic heart valves.
{"title":"Zwitterionic hydrogel coatings enhance anti-fouling and anti-thrombogenicity of decellularized swim bladder for bioprosthetic heart valves","authors":"Haiyue Ren , Zhicheng Pan , Jinfeng Yuan , Jing Liu","doi":"10.1016/j.colsurfb.2025.115349","DOIUrl":"10.1016/j.colsurfb.2025.115349","url":null,"abstract":"<div><div>Glutaraldehyde cross-linked bovine/porcine pericardium has been used as prosthetic heart valves (BHVs), while residual aldehyde groups induce cytotoxicity, thrombus formation, and progressive calcification deposition, which ultimately limits instrument life span to 10–15 years. We innovatively designed a multifunctional zwitterionic hydrogel coating copolymer poly (SBMA-co-DMEMAm) on swim bladder-derived material, which is composed of hydrophilic monomer SBMA and aldehyde-containing monomer DMEMAm; besides the aldehyde group can crosslink fish bladder tissue and improve the mechanical properties. This multifunctional hydrophilic polymer coatings provide effective anti-fouling properties, better anti-platelet adsorption and anti-thrombotic properties in <em>in vitro</em> blood compatibility evaluation. In addition, it showed better anti-inflammatory and anti-calcification properties in rat subcutaneous implantation. Thus, this study presents a versatile zwitterionic hydrogel coating strategy that simultaneously endows decellularized swim bladder with superior anti-fouling, hemocompatibility, cytocompatibility, and anti-thrombogenicity, offering a comprehensive solution for durable bioprosthetic heart valves.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"260 ","pages":"Article 115349"},"PeriodicalIF":5.6,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145831802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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