Pub Date : 2026-01-13DOI: 10.1016/j.colsurfb.2026.115447
Seung Hyeon Kim , Sangmin Lee , Han-Jun Kim , Jae Seo Lee , Sang Jin Lee , Haram Nah , Sung Jun Min , Jae Beum Bang , Dae Hyeok Yang , Won Jong Kim , Il Keun Kwon , Dong Nyoung Heo
This study entailed the development of a nitric oxide (NO)-releasing, 3D-printable, heat-resistant polymer scaffold to support angiogenesis and osteogenesis for tissue engineering applications. A scaffold synthesized with poly(nitrocarbonate)-poly(ε-caprolactone) random copolymer (PNC-ran-PCL) enabled controlled NO release, addressing the rapid diffusion limitations found in conventional NO delivery systems. The thermoplastic properties of PNC-ran-PCL facilitate the creation of biomimetic structures tailored to patient-specific requirements. In vitro assessments showed that the scaffold was non-toxic and that it promoted the proliferation and activity of human umbilical vein endothelial cells, supporting angiogenic functions. In vivo studies using a rat calvarial defect model further demonstrated enhanced vascularization and initial bone formation around the scaffold, highlighting its potential to promote early bone regeneration. The proposed NO-releasing scaffold, which is capable of low-temperature extrusion, has promising applications in in-situ tissue engineering and provides a versatile solution for large-area tissue repair. Further studies on optimized NO-release kinetics are required to enhance the efficacy of the proposed scaffold in osteogenic applications.
{"title":"3D-printable, heat-resistant polycaprolactone-based polymer scaffold for sustained NO release in tissue engineering applications","authors":"Seung Hyeon Kim , Sangmin Lee , Han-Jun Kim , Jae Seo Lee , Sang Jin Lee , Haram Nah , Sung Jun Min , Jae Beum Bang , Dae Hyeok Yang , Won Jong Kim , Il Keun Kwon , Dong Nyoung Heo","doi":"10.1016/j.colsurfb.2026.115447","DOIUrl":"10.1016/j.colsurfb.2026.115447","url":null,"abstract":"<div><div>This study entailed the development of a nitric oxide (NO)-releasing, 3D-printable, heat-resistant polymer scaffold to support angiogenesis and osteogenesis for tissue engineering applications. A scaffold synthesized with poly(nitrocarbonate)-poly(ε-caprolactone) random copolymer (PNC-<em>ran</em>-PCL) enabled controlled NO release, addressing the rapid diffusion limitations found in conventional NO delivery systems. The thermoplastic properties of PNC-<em>ran</em>-PCL facilitate the creation of biomimetic structures tailored to patient-specific requirements. <em>In vitro</em> assessments showed that the scaffold was non-toxic and that it promoted the proliferation and activity of human umbilical vein endothelial cells, supporting angiogenic functions. <em>In vivo</em> studies using a rat calvarial defect model further demonstrated enhanced vascularization and initial bone formation around the scaffold, highlighting its potential to promote early bone regeneration. The proposed NO-releasing scaffold, which is capable of low-temperature extrusion, has promising applications in in-situ tissue engineering and provides a versatile solution for large-area tissue repair. Further studies on optimized NO-release kinetics are required to enhance the efficacy of the proposed scaffold in osteogenic applications.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"261 ","pages":"Article 115447"},"PeriodicalIF":5.6,"publicationDate":"2026-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145975465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-12DOI: 10.1016/j.colsurfb.2026.115438
Ziyang Bai , Yifan Zhao , Yajuan Gong , Meijun Du , Wenjun Zhang , Ke Zhang , Yongchao Zhi , Yanan Nie , Xia Li , Xiuping Wu , Bing Li
Infected wounds present major clinical challenges due to excessive bacterial colonization, sustained inflammation, and impaired tissue repair. To address these barriers, we developed a temperature-responsive hydrogel delivery system incorporating watermelon-derived extracellular vesicles (wEV), antimicrobial peptide-engineered for the topical treatment of infected wounds. wEVs contained terpenoids, flavonoids, alkaloids, and proteins with intrinsic anti-inflammatory and pro-regenerative activity. To enhance stability and antibacterial potency, antimicrobial peptides (AMP) were conjugated to wEVs via mussel derivatives, producing wEV-AMP. These were embedded in a temperature-responsive Pluronic F127/chitosan hydrogel that gels at 37 °C for wound coverage and controlled release. In vitro, PF127/CS+wEV-AMP inhibited > 95 % of Staphylococcus aureus and Escherichia coli, suppressed biofilms, reduced inflammatory cytokines, and enhanced fibroblast migration. In infected rat wounds, healing rate reached ∼60 % by day 5 and nearly complete closure by day 14, with greater collagen deposition and M2 macrophage polarization. This multifunctional hydrogel integrates antimicrobial, immunomodulatory, and regenerative effects, offering strong potential for infectious wound treatment.
{"title":"Temperature-responsive hydrogel delivery of antimicrobial peptide engineered watermelon-derived extracellular vesicles enables sequential infection control and wound healing","authors":"Ziyang Bai , Yifan Zhao , Yajuan Gong , Meijun Du , Wenjun Zhang , Ke Zhang , Yongchao Zhi , Yanan Nie , Xia Li , Xiuping Wu , Bing Li","doi":"10.1016/j.colsurfb.2026.115438","DOIUrl":"10.1016/j.colsurfb.2026.115438","url":null,"abstract":"<div><div>Infected wounds present major clinical challenges due to excessive bacterial colonization, sustained inflammation, and impaired tissue repair. To address these barriers, we developed a temperature-responsive hydrogel delivery system incorporating watermelon-derived extracellular vesicles (wEV), antimicrobial peptide-engineered for the topical treatment of infected wounds. wEVs contained terpenoids, flavonoids, alkaloids, and proteins with intrinsic anti-inflammatory and pro-regenerative activity. To enhance stability and antibacterial potency, antimicrobial peptides (AMP) were conjugated to wEVs via mussel derivatives, producing wEV-AMP. These were embedded in a temperature-responsive Pluronic F127/chitosan hydrogel that gels at 37 °C for wound coverage and controlled release. <em>In vitro</em>, PF127/CS+wEV-AMP inhibited > 95 % of <em>Staphylococcus aureus</em> and <em>Escherichia coli</em>, suppressed biofilms, reduced inflammatory cytokines, and enhanced fibroblast migration. In infected rat wounds, healing rate reached ∼60 % by day 5 and nearly complete closure by day 14, with greater collagen deposition and M2 macrophage polarization. This multifunctional hydrogel integrates antimicrobial, immunomodulatory, and regenerative effects, offering strong potential for infectious wound treatment.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"261 ","pages":"Article 115438"},"PeriodicalIF":5.6,"publicationDate":"2026-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145975464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-12DOI: 10.1016/j.colsurfb.2026.115437
Pravin D. Patil , Niharika Gargate , Manishkumar S. Tiwari , Ajay N. Phirke , Shamraja S. Nadar
An amino acid-assisted fabrication of enzyme-MOF composites represents a novel approach for enhancing biocatalyst performance through molecular-level interactions. The flexible roles of amino acids as modulators, linkers, defect inducers, and microenvironmental regulators inside MOF systems for enzyme immobilization are extensively investigated in this review. This review examines explicitly amino acids in four key aspects: (i) modulation of MOF attributes including physico-chemical stability, surface functionalization and microenvironment, morphology, and defect engineering; (ii) enhancement of immobilized enzyme properties such as catalytic activity, stability, kinetic parameters, and reusability; (iii) their role as organic cross-linkers in univariate and multivariate amino acid-based MOFs; and (iv) mechanistic insights into nucleation, self-assembly, and enzyme-MOF interactions. Mechanistic knowledge helps define several roles that natural amino acids play in guiding MOF nucleation, fostering self-assembly, and enhancing catalytic activity. Their capacity to modify surface chemistry, maintain enzyme conformation, and increase active-site exposure is especially underlined to improve the biocatalyst's thermal, chemical, and reusability profiles. Furthermore, amino acid-mediated defect engineering of MOF can enhance pore structure and loading efficiency. Despite scattered experimental demonstrations, this review views amino acids as next-generation molecular tools for tailoring MOF-enzyme systems, providing a template for the rational construction of composites. The work concludes by identifying current limitations and outlining future opportunities in amino acid-guided bio-hybrid catalyst development for industrial biotransformation.
{"title":"Amino acid-based modulation of enzyme-metal organic frameworks (MOFs) composites: A conceptual perspective","authors":"Pravin D. Patil , Niharika Gargate , Manishkumar S. Tiwari , Ajay N. Phirke , Shamraja S. Nadar","doi":"10.1016/j.colsurfb.2026.115437","DOIUrl":"10.1016/j.colsurfb.2026.115437","url":null,"abstract":"<div><div>An amino acid-assisted fabrication of enzyme-MOF composites represents a novel approach for enhancing biocatalyst performance through molecular-level interactions. The flexible roles of amino acids as modulators, linkers, defect inducers, and microenvironmental regulators inside MOF systems for enzyme immobilization are extensively investigated in this review. This review examines explicitly amino acids in four key aspects: (i) modulation of MOF attributes including physico-chemical stability, surface functionalization and microenvironment, morphology, and defect engineering; (ii) enhancement of immobilized enzyme properties such as catalytic activity, stability, kinetic parameters, and reusability; (iii) their role as organic cross-linkers in univariate and multivariate amino acid-based MOFs; and (iv) mechanistic insights into nucleation, self-assembly, and enzyme-MOF interactions. Mechanistic knowledge helps define several roles that natural amino acids play in guiding MOF nucleation, fostering self-assembly, and enhancing catalytic activity. Their capacity to modify surface chemistry, maintain enzyme conformation, and increase active-site exposure is especially underlined to improve the biocatalyst's thermal, chemical, and reusability profiles. Furthermore, amino acid-mediated defect engineering of MOF can enhance pore structure and loading efficiency. Despite scattered experimental demonstrations, this review views amino acids as next-generation molecular tools for tailoring MOF-enzyme systems, providing a template for the rational construction of composites. The work concludes by identifying current limitations and outlining future opportunities in amino acid-guided bio-hybrid catalyst development for industrial biotransformation.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"261 ","pages":"Article 115437"},"PeriodicalIF":5.6,"publicationDate":"2026-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145975462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-12DOI: 10.1016/j.colsurfb.2026.115435
Fábio M. Carvalho , Marta Lima , Iffat Shahzad , Kathryn A. Whitehead , Luciana C. Gomes , Filipe J. Mergulhão
Pathogen contamination of food contact surfaces poses serious public health and economic risks. Bacterial binding to these surfaces enhances microbial resistance to cleaning and disinfection, thus requiring novel antifouling strategies. This study explored a biomimetic approach to surface engineering by replicating the microtopographies of four plant leaves – Tenderheart (TH), Cauliflower (CF), White Cabbage (WC) and Leek (L) – onto wax and silicone substrates, aiming to reduce Escherichia coli binding. The biomimetic surfaces were fabricated using a moulding technique and characterized for topography (Optical Profilometry and Scanning Electron Microscopy), wettability (Water Contact Angle), and surface chemistry (Raman Spectroscopy), before and after conditioning with a casein film. Antifouling performance was evaluated through spray-and-wash (Attachment) and 1-hour static immersion (Retention) assays. All biomimetic surfaces exhibited significantly higher roughness and lower wettability than the flat controls. Silicone biomimetic surfaces, being less wettable, generally outperformed the wax surfaces, reducing bacterial attachment by up to 88 % (CF) and retention by up to 82 % (WC). The L topography consistently demonstrated strong anti-binding activity against E. coli attachment, whereas the WC surface proved particularly effective in reducing bacterial numbers in retention assays. Although conditioning the surfaces with casein partially masked the surface features and increased their wettability, silicone biomimetic surfaces (WC and L) maintained a significant antifouling efficacy (up to 90 % reduction). Overall, higher roughness and low wettability synergistically hindered bacterial colonization. These findings support the potential of nature-inspired surfaces as a promising strategy to minimize bacterial contamination in food processing equipment.
{"title":"Vegetable-inspired biomimetic surfaces for preventing Escherichia coli binding in the food industry","authors":"Fábio M. Carvalho , Marta Lima , Iffat Shahzad , Kathryn A. Whitehead , Luciana C. Gomes , Filipe J. Mergulhão","doi":"10.1016/j.colsurfb.2026.115435","DOIUrl":"10.1016/j.colsurfb.2026.115435","url":null,"abstract":"<div><div>Pathogen contamination of food contact surfaces poses serious public health and economic risks. Bacterial binding to these surfaces enhances microbial resistance to cleaning and disinfection, thus requiring novel antifouling strategies. This study explored a biomimetic approach to surface engineering by replicating the microtopographies of four plant leaves – Tenderheart (TH), Cauliflower (CF), White Cabbage (WC) and Leek (L) – onto wax and silicone substrates, aiming to reduce <em>Escherichia coli</em> binding. The biomimetic surfaces were fabricated using a moulding technique and characterized for topography (Optical Profilometry and Scanning Electron Microscopy), wettability (Water Contact Angle), and surface chemistry (Raman Spectroscopy), before and after conditioning with a casein film. Antifouling performance was evaluated through spray-and-wash (Attachment) and 1-hour static immersion (Retention) assays. All biomimetic surfaces exhibited significantly higher roughness and lower wettability than the flat controls. Silicone biomimetic surfaces, being less wettable, generally outperformed the wax surfaces, reducing bacterial attachment by up to 88 % (CF) and retention by up to 82 % (WC). The L topography consistently demonstrated strong anti-binding activity against <em>E. coli</em> attachment, whereas the WC surface proved particularly effective in reducing bacterial numbers in retention assays. Although conditioning the surfaces with casein partially masked the surface features and increased their wettability, silicone biomimetic surfaces (WC and L) maintained a significant antifouling efficacy (up to 90 % reduction). Overall, higher roughness and low wettability synergistically hindered bacterial colonization. These findings support the potential of nature-inspired surfaces as a promising strategy to minimize bacterial contamination in food processing equipment.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"261 ","pages":"Article 115435"},"PeriodicalIF":5.6,"publicationDate":"2026-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145974861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-10DOI: 10.1016/j.colsurfb.2026.115434
Fengzhu Yang , Jintao Deng , Xinyu Yu , Wenhao Fu , Wenlong Sun , Zhengbao Xu , Xinhua Song , Chao Wang , Qingqing Du , Meng Wang
The lysosomes of drug-resistant tumor cells transport doxorubicin (DOX) and its nanocarriers into lysosomes through a sequestration mechanism, making it difficult for DOX to reach the therapeutic concentration. In this study, a nanodrug delivery system (Dp/DGPP) capable of overcoming tumor lysosomal resistance was developed. The system consists of three parts: graphene oxide (GO)-Se-Se-DOX, polyethyleneimine-pluronic F127 (PEI-PF127) for improved biocompatibility, and Dp44mT for amplifying reactive oxygen species (ROS) and disrupting lysosomes. After entering MCF-7/ADR tumor cells, loading Dp44mT can reduce the permeability of lysosomal membranes by increasing the level of ROS. DOX subsequently escapes from lysosomes and breaks the diselenide bond to complete its release. The experiment proved that Dp/DGPP exhibited a significant ROS-dependent response to the release of DOX. Compared with the other groups, it had greater cytotoxicity, and the IC50 value of DOX against MCF-7/ADR cells was as low as 6.71 μM. Compared with the DOX group, the Dp/DGPP group exhibited greater DOX accumulation in MCF-7/ADR cells, with 1.5-fold greater fluorescence at 4 h. Meanwhile, Dp/DGPP can cause lysosomal dysfunction by reducing lysosomal membrane permeability. Western blot results revealed that the expression of Pgp protein decreased in the Dp/DGPP group, whereas the expression of autophagy related LC3-II and P62 protein increased, which confirmed that autophagic flux was blocked. This reduces potential drug resistance and promotes cell death. In addition, the Dp/DGPP group achieved a 70 % inhibition rate in MCF-7/ADR tumors in vivo. This nanodrug delivery system provides a potential strategy to overcome tumor DOX resistance via lysosomes.
{"title":"Lysosome-targeted ROS-responsive graphene oxide-based drug delivery system to overcome tumor DOX resistance","authors":"Fengzhu Yang , Jintao Deng , Xinyu Yu , Wenhao Fu , Wenlong Sun , Zhengbao Xu , Xinhua Song , Chao Wang , Qingqing Du , Meng Wang","doi":"10.1016/j.colsurfb.2026.115434","DOIUrl":"10.1016/j.colsurfb.2026.115434","url":null,"abstract":"<div><div>The lysosomes of drug-resistant tumor cells transport doxorubicin (DOX) and its nanocarriers into lysosomes through a sequestration mechanism, making it difficult for DOX to reach the therapeutic concentration. In this study, a nanodrug delivery system (Dp/DGPP) capable of overcoming tumor lysosomal resistance was developed. The system consists of three parts: graphene oxide (GO)-Se-Se-DOX, polyethyleneimine-pluronic F127 (PEI-PF127) for improved biocompatibility, and Dp44mT for amplifying reactive oxygen species (ROS) and disrupting lysosomes. After entering MCF-7/ADR tumor cells, loading Dp44mT can reduce the permeability of lysosomal membranes by increasing the level of ROS. DOX subsequently escapes from lysosomes and breaks the diselenide bond to complete its release. The experiment proved that Dp/DGPP exhibited a significant ROS-dependent response to the release of DOX. Compared with the other groups, it had greater cytotoxicity, and the IC50 value of DOX against MCF-7/ADR cells was as low as 6.71 μM. Compared with the DOX group, the Dp/DGPP group exhibited greater DOX accumulation in MCF-7/ADR cells, with 1.5-fold greater fluorescence at 4 h. Meanwhile, Dp/DGPP can cause lysosomal dysfunction by reducing lysosomal membrane permeability. Western blot results revealed that the expression of Pgp protein decreased in the Dp/DGPP group, whereas the expression of autophagy related LC3-II and P62 protein increased, which confirmed that autophagic flux was blocked. This reduces potential drug resistance and promotes cell death. In addition, the Dp/DGPP group achieved a 70 % inhibition rate in MCF-7/ADR tumors in vivo. This nanodrug delivery system provides a potential strategy to overcome tumor DOX resistance via lysosomes.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"261 ","pages":"Article 115434"},"PeriodicalIF":5.6,"publicationDate":"2026-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145975458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-10DOI: 10.1016/j.colsurfb.2026.115436
C. Arias-Mainer , F. Romero-Gavilán , L. Abenia-Artigas , I. García-Arnáez , O. Amorrotu , M. Azkargorta , F. Elortza , M. Gurruchaga , I. Goñi , J. Suay
Manganese (Mn), an essential trace element involved in bone metabolism, plays a crucial role in key biological functions, including the regulation of cell adhesion, modulation of immune responses, and promotion of osteogenesis. In this study, sol-gel coatings with increasing concentrations of MnCl₂ (0.5, 1 and 1.5 % wt) were synthesised and applied onto titanium (Ti) surfaces. The materials were characterised physicochemically, and in vitro responses were assessed using human osteoblasts (HOb) and THP-1-derived macrophages. Protein adsorption from human serum was analysed by nLC-MS/MS. The incorporation of MnCl₂ did not disrupt the sol-gel silica network and enabled a sustained release of Mn²⁺ ions, and all coatings showed good biocompatibility with no cytotoxicity. Immune response analysis revealed that 0.5Mn promoted anti-inflammatory markers (IL-10, TGF-β), while 1Mn and 1.5Mn induced strong proinflammatory profiles, reflected in increased TNF-α and IL-1β. Proteomics identified preferential adsorption of immune-related proteins such as complement components, ficolins, acute-phase proteins and apolipoproteins to 1Mn and 1.5Mn. Conversely, 0.5Mn enhanced the adsorption of proteins linked to anti-inflammatory effects and oxidative stress regulation. Mn-doped surfaces also enhanced gene expression related to cell adhesion (CTNNB1, ITG1B, PTK2) and osteogenic markers (RUNX2, BMP2, BGLAP), particularly on 1.5Mn, correlating with increased calcium deposition and adsorption of mineralisation-related proteins (FETUA, ECM1, IGF2). All Mn sol-gel coatings promoted the coagulation cascade through increased adsorption of FA9, FA12 and ZPI. These results demonstrate the capacity of Mn-doped sol-gel coatings to modulate immune and osteogenic responses, underscoring the relevance of optimising Mn concentration to improve bone–implant integration.
{"title":"Incorporating Mn²⁺ ions in bioactive sol-gel coatings: Impact on cell adhesion, inflammation and bone regeneration","authors":"C. Arias-Mainer , F. Romero-Gavilán , L. Abenia-Artigas , I. García-Arnáez , O. Amorrotu , M. Azkargorta , F. Elortza , M. Gurruchaga , I. Goñi , J. Suay","doi":"10.1016/j.colsurfb.2026.115436","DOIUrl":"10.1016/j.colsurfb.2026.115436","url":null,"abstract":"<div><div>Manganese (Mn), an essential trace element involved in bone metabolism, plays a crucial role in key biological functions, including the regulation of cell adhesion, modulation of immune responses, and promotion of osteogenesis. In this study, sol-gel coatings with increasing concentrations of MnCl₂ (0.5, 1 and 1.5 % wt) were synthesised and applied onto titanium (Ti) surfaces. The materials were characterised physicochemically, and <em>in vitro</em> responses were assessed using human osteoblasts (HOb) and THP-1-derived macrophages. Protein adsorption from human serum was analysed by nLC-MS/MS. The incorporation of MnCl₂ did not disrupt the sol-gel silica network and enabled a sustained release of Mn²⁺ ions, and all coatings showed good biocompatibility with no cytotoxicity. Immune response analysis revealed that 0.5Mn promoted anti-inflammatory markers (IL-10, TGF-β), while 1Mn and 1.5Mn induced strong proinflammatory profiles, reflected in increased TNF-α and IL-1β. Proteomics identified preferential adsorption of immune-related proteins such as complement components, ficolins, acute-phase proteins and apolipoproteins to 1Mn and 1.5Mn. Conversely, 0.5Mn enhanced the adsorption of proteins linked to anti-inflammatory effects and oxidative stress regulation. Mn-doped surfaces also enhanced gene expression related to cell adhesion (CTNNB1, ITG1B, PTK2) and osteogenic markers (RUNX2, BMP2, BGLAP), particularly on 1.5Mn, correlating with increased calcium deposition and adsorption of mineralisation-related proteins (FETUA, ECM1, IGF2). All Mn sol-gel coatings promoted the coagulation cascade through increased adsorption of FA9, FA12 and ZPI. These results demonstrate the capacity of Mn-doped sol-gel coatings to modulate immune and osteogenic responses, underscoring the relevance of optimising Mn concentration to improve bone–implant integration.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"261 ","pages":"Article 115436"},"PeriodicalIF":5.6,"publicationDate":"2026-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145964831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-09DOI: 10.1016/j.colsurfb.2026.115426
Zhengguang Pu , Wang Gong , Haoming Wu , Hao Zhou , YiXuan Lan , Yingying Chen , Xiuxing Liu , Zhineng Lu , Xiaoyu Du , Xinlei Yang , Shuhao Yang , Wanyue Feng , Chao Peng , Hai Lan , Xulin Hu
Musculoskeletal disorders (MSDs), such as osteoporosis, cartilage degeneration, and tendon injuries, are common worldwide and have a significant impact on patients' quality of life. These conditions are frequently associated with aging, trauma, or chronic diseases. They are typically characterized by slow and limited self-repair, particularly in tissues like cartilage and tendons, which exhibit low regenerative potential. Traditional treatment methods, such as implantable scaffolds, often face challenges related to incomplete tissue integration and inadequate adaptation to dynamic biomechanical conditions. Shape memory scaffolds (SMSs) have emerged as promising candidates for repairing musculoskeletal tissues, due to their unique ability to respond to external stimuli, such as temperature, light, and pH. These materials can adapt to irregular tissue defects and dynamically adjust to biomechanical requirements during the healing process, thereby potentially supporting tissue regeneration. This review discusses the advantages of SMSs in musculoskeletal system reconstruction, emphasizing their mechanical responsiveness, adaptability, and bioactive potential. Furthermore, recent advancements in SMS-based scaffolds and the role of 4D printing in enhancing their functionality are systematically reviewed. Finally, we propose future research directions aimed at enabling more effective and personalized treatments for MSDs.
{"title":"Innovative applications and future challenges of shape memory scaffolds for functional reconstruction in diseases of the musculoskeletal system","authors":"Zhengguang Pu , Wang Gong , Haoming Wu , Hao Zhou , YiXuan Lan , Yingying Chen , Xiuxing Liu , Zhineng Lu , Xiaoyu Du , Xinlei Yang , Shuhao Yang , Wanyue Feng , Chao Peng , Hai Lan , Xulin Hu","doi":"10.1016/j.colsurfb.2026.115426","DOIUrl":"10.1016/j.colsurfb.2026.115426","url":null,"abstract":"<div><div>Musculoskeletal disorders (MSDs), such as osteoporosis, cartilage degeneration, and tendon injuries, are common worldwide and have a significant impact on patients' quality of life. These conditions are frequently associated with aging, trauma, or chronic diseases. They are typically characterized by slow and limited self-repair, particularly in tissues like cartilage and tendons, which exhibit low regenerative potential. Traditional treatment methods, such as implantable scaffolds, often face challenges related to incomplete tissue integration and inadequate adaptation to dynamic biomechanical conditions. Shape memory scaffolds (SMSs) have emerged as promising candidates for repairing musculoskeletal tissues, due to their unique ability to respond to external stimuli, such as temperature, light, and pH. These materials can adapt to irregular tissue defects and dynamically adjust to biomechanical requirements during the healing process, thereby potentially supporting tissue regeneration. This review discusses the advantages of SMSs in musculoskeletal system reconstruction, emphasizing their mechanical responsiveness, adaptability, and bioactive potential. Furthermore, recent advancements in SMS-based scaffolds and the role of 4D printing in enhancing their functionality are systematically reviewed. Finally, we propose future research directions aimed at enabling more effective and personalized treatments for MSDs.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"261 ","pages":"Article 115426"},"PeriodicalIF":5.6,"publicationDate":"2026-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145964880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Chronic wounds require dressings that manage exudate, conform to soft tissue, provide mechanical support, and deliver intrinsic bioactivity. Here, we report conductive, hydrogel-like porous polyhydroxyalkanoate (PHA) monoliths dressings coated with polypyrrole (PPy) using oxidative chemical vapor deposition (oCVD). Porous PHA substrates were prepared from polyhydroxybutyrate (PHB) and a P4HB-containing copolymer by thermally induced phase separation and were uniformly functionalized throughout their three-dimensional architecture by this solvent-free process. The resulting PHA/PPy porous monoliths combine high water uptake with electrical conductivity and biological activity. They exhibit a swelling ratio of ∼250 %, maintaining a moist environment while preserving viscoelastic integrity. Sheet resistance ranges from 26 to 86 kΩ/sq, enabling platforms for electrical sensing in tissue repair. The composites do not induce cytotoxicity and exhibit intrinsic radical-scavenging capacity and antibacterial activity against both Gram-positive and Gram-negative bacteria. These properties are achieved without chemical derivatization of the PHA matrix. The hydrophobic PHA core provides mechanical robustness, while the conformal PPy layer imparts conductivity and bioactivity. Overall, this oCVD route provides a scalable, solvent-free strategy to engineer multifunctional, hydrogel-like porous monolith dressings that integrate moisture management, mechanical resilience, electrical conduction, and inherent antioxidant and antimicrobial activity. These features position the developed materials as promising bioactive and bioelectronic wound dressings and soft tissue interfaces.
{"title":"Conformal polypyrrole biointerfaces on porous PHA monoliths via oxidative chemical vapor deposition","authors":"Adriana Kovalcik , Nicole Cernekova , Fika Fauzi , Ranjita K. Bose , Zdenko Spitalsky , Zuzana Kadlecova , Lucy Vojtova , Zdenka Víchová , Petr Humpolíček , Patrycja Bober","doi":"10.1016/j.colsurfb.2026.115417","DOIUrl":"10.1016/j.colsurfb.2026.115417","url":null,"abstract":"<div><div>Chronic wounds require dressings that manage exudate, conform to soft tissue, provide mechanical support, and deliver intrinsic bioactivity. Here, we report conductive, hydrogel-like porous polyhydroxyalkanoate (PHA) monoliths dressings coated with polypyrrole (PPy) using oxidative chemical vapor deposition (oCVD). Porous PHA substrates were prepared from polyhydroxybutyrate (PHB) and a P4HB-containing copolymer by thermally induced phase separation and were uniformly functionalized throughout their three-dimensional architecture by this solvent-free process. The resulting PHA/PPy porous monoliths combine high water uptake with electrical conductivity and biological activity. They exhibit a swelling ratio of ∼250 %, maintaining a moist environment while preserving viscoelastic integrity. Sheet resistance ranges from 26 to 86 kΩ/sq, enabling platforms for electrical sensing in tissue repair. The composites do not induce cytotoxicity and exhibit intrinsic radical-scavenging capacity and antibacterial activity against both <em>Gram-positive</em> and <em>Gram-negative</em> bacteria. These properties are achieved without chemical derivatization of the PHA matrix. The hydrophobic PHA core provides mechanical robustness, while the conformal PPy layer imparts conductivity and bioactivity. Overall, this oCVD route provides a scalable, solvent-free strategy to engineer multifunctional, hydrogel-like porous monolith dressings that integrate moisture management, mechanical resilience, electrical conduction, and inherent antioxidant and antimicrobial activity. These features position the developed materials as promising bioactive and bioelectronic wound dressings and soft tissue interfaces.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"261 ","pages":"Article 115417"},"PeriodicalIF":5.6,"publicationDate":"2026-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145964868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-09DOI: 10.1016/j.colsurfb.2026.115430
Kyeongjung Kim , Se-woon Choe , Jae-Young Je , Min-Jin Hwang , Yoonhang Lee , Do-Hyung Kim , Kwangcheol Casey Jeong , Soon-Do Yoon
Transdermal drug delivery systems (TDDS) using microneedles (MNs) patches have shown promise for improved therapeutic outcomes. In this study, acyclovir (ACV)-loaded MNs patches for herpes simplex virus (HSV) therapy were prepared using mungbean starch (MBS), polyvinyl alcohol (PVA), and plasticizers (arginine and mannitol), and their physicochemical properties, ACV release behavior, antimicrobial activity, and biodegradation, cell viability, and antiviral efficacy were investigated. The MNs exhibited compression forces of 1.30 – 4.80 N/needle and a pyramidal square shape with a length of 620–640 μm, ensuring efficient skin penetration. The ACV release (%) from the ACV-loaded MNs patches during an artificial skin test was found to be 2.50 – 4.32 times higher than that from ACV-loaded biomaterial as the film-type formulation. Additionally, over 98.0 % of ACV was released from the prepared MNs patches within 80 min. The ACV release mechanism was analyzed using zero-order, first-order, Higuchi, Fickian diffusion, and Korsmeyer-Peppas models, which revealed a Fickian diffusion mechanism. Visualization of intradermal drug release were conducted using ACV- and riboflavin-loaded MNs patches on agar blocks and pig ears/agar block models. Biodegradability, cell viability, and antiviral studies further demonstrated the potential of MNs patches as a TDDS. These results suggest that the prepared MNs patches are promising candidates for transdermal HSV therapy.
{"title":"Characterizations and controlled drug release behavior of acyclovir-loaded starch-based microneedles patches for transdermal herpes simplex virus therapy","authors":"Kyeongjung Kim , Se-woon Choe , Jae-Young Je , Min-Jin Hwang , Yoonhang Lee , Do-Hyung Kim , Kwangcheol Casey Jeong , Soon-Do Yoon","doi":"10.1016/j.colsurfb.2026.115430","DOIUrl":"10.1016/j.colsurfb.2026.115430","url":null,"abstract":"<div><div>Transdermal drug delivery systems (TDDS) using microneedles (MNs) patches have shown promise for improved therapeutic outcomes. In this study, acyclovir (ACV)-loaded MNs patches for herpes simplex virus (HSV) therapy were prepared using mungbean starch (MBS), polyvinyl alcohol (PVA), and plasticizers (arginine and mannitol), and their physicochemical properties, ACV release behavior, antimicrobial activity, and biodegradation, cell viability, and antiviral efficacy were investigated. The MNs exhibited compression forces of 1.30 – 4.80 N/needle and a pyramidal square shape with a length of 620–640 μm, ensuring efficient skin penetration. The ACV release (%) from the ACV-loaded MNs patches during an artificial skin test was found to be 2.50 – 4.32 times higher than that from ACV-loaded biomaterial as the film-type formulation. Additionally, over 98.0 % of ACV was released from the prepared MNs patches within 80 min. The ACV release mechanism was analyzed using zero-order, first-order, Higuchi, Fickian diffusion, and Korsmeyer-Peppas models, which revealed a Fickian diffusion mechanism. Visualization of intradermal drug release were conducted using ACV- and riboflavin-loaded MNs patches on agar blocks and pig ears/agar block models. Biodegradability, cell viability, and antiviral studies further demonstrated the potential of MNs patches as a TDDS. These results suggest that the prepared MNs patches are promising candidates for transdermal HSV therapy.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"261 ","pages":"Article 115430"},"PeriodicalIF":5.6,"publicationDate":"2026-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145964875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-09DOI: 10.1016/j.colsurfb.2026.115432
Bo Zhao , Xinjian Cheng , Junyu Chen , Chenghua Zhang , Jun Liu
The integration of bioimaging and photodynamic therapy (PDT) joins real-time visualization with spatially controlled therapeutic activation, thus presenting a promising theranostic platform for precision oncology. However, the clinical translation of conventional photosensitizers suffers from limited tissue penetration and potential photodamage. To overcome these shortcomings, we proposed a NIR responsive system in this work. A naphthalimide-based small molecule (NQL) was synthesized, followed by oligo-chitosan conjugation through Schiff base formation, yielding the water-soluble probe CS-NQL. CS-NQL exhibits a twisted intramolecular charge transfer (TICT) state. It also has the capability to target lysosomes. This enables CS-NQL to respond to environmental viscosity, with target lysosomes. The CS-NQL was then electrostatically self-assembled with DNA to form nanoparticles (CS-NQL@DNA NPs). These nanoparticles (NPs) can generate reactive oxygen species (ROS) under 660 nm laser irradiation, exhibiting photosensitivity. Further detection using commercial reagents revealed this photosensitivity stems via Type I process. Critically, this oxygen-independent mechanism retained the ability to generate ROS under hypoxia, overcoming the limitations of the tumor microenvironment. Under 660 nm laser irradiation (0.4 W/cm2) for 20 min, NPs generate ROS, leading to apoptosis in over 90 % of HeLa cells and demonstrating antitumor effects. In vivo experiments, it is demonstrated that the NPs exhibited PDT after 30 min of 660 nm laser irradiation, achieving significant tumor suppression.
{"title":"Chitosan-based bimodal photosensitive system: Synergistic realization of visible light viscosity imaging and near-infrared type I photodynamic therapy","authors":"Bo Zhao , Xinjian Cheng , Junyu Chen , Chenghua Zhang , Jun Liu","doi":"10.1016/j.colsurfb.2026.115432","DOIUrl":"10.1016/j.colsurfb.2026.115432","url":null,"abstract":"<div><div>The integration of bioimaging and photodynamic therapy (PDT) joins real-time visualization with spatially controlled therapeutic activation, thus presenting a promising theranostic platform for precision oncology. However, the clinical translation of conventional photosensitizers suffers from limited tissue penetration and potential photodamage. To overcome these shortcomings, we proposed a NIR responsive system in this work. A naphthalimide-based small molecule (NQL) was synthesized, followed by oligo-chitosan conjugation through Schiff base formation, yielding the water-soluble probe CS-NQL. CS-NQL exhibits a twisted intramolecular charge transfer (TICT) state. It also has the capability to target lysosomes. This enables CS-NQL to respond to environmental viscosity, with target lysosomes. The CS-NQL was then electrostatically self-assembled with DNA to form nanoparticles (CS-NQL@DNA NPs). These nanoparticles (NPs) can generate reactive oxygen species (ROS) under 660 nm laser irradiation, exhibiting photosensitivity. Further detection using commercial reagents revealed this photosensitivity stems via Type I process. Critically, this oxygen-independent mechanism retained the ability to generate ROS under hypoxia, overcoming the limitations of the tumor microenvironment. Under 660 nm laser irradiation (0.4 W/cm<sup>2</sup>) for 20 min, NPs generate ROS, leading to apoptosis in over 90 % of HeLa cells and demonstrating antitumor effects. In vivo experiments, it is demonstrated that the NPs exhibited PDT after 30 min of 660 nm laser irradiation, achieving significant tumor suppression.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"261 ","pages":"Article 115432"},"PeriodicalIF":5.6,"publicationDate":"2026-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145924118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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