Pub Date : 2025-01-20DOI: 10.1016/j.colsurfb.2025.114528
Longyu Wang , Yichen Li , Pingge Jiang , Hao Bai , Chengfan Wu , Qi Shuai , Yunfeng Yan
Lipid nanoparticles (LNPs) have shown promising performance in mRNA delivery. Nevertheless, a thorough understanding of the relationship between mRNA delivery efficacy and the structure of LNPs remains imperative. In this study, we systematically investigated the effects of additional hydrophobic amines on the physicochemical properties of mRNA LNPs and their delivery efficacy. The results indicated that this influence depended on the chemical structure of the additional amines and the structure of the lipid carriers. The appropriate addition of the hydrophobic amine 2C8 to lipid carriers with structural 2C8 or 2C6 tails significantly increased their mRNA delivery efficiency. In contrast, the addition of hydrophobic amine C18 to LNPs resulted in a decrease in mRNA delivery efficiency, while the addition of hydrophobic amines 2C6 and C8, as well as alkanes C12' and C16', had relatively little effect on mRNA delivery. Further investigations demonstrated that the appropriate addition of 2C8 could reduce LNP size, moderate internal hydrophobicity and LNP stability, facilitate mRNA release, enhance cellular uptake, and improve intracellular transportation of LNPs, thereby achieving superior mRNA delivery efficiency. These findings highlight the important role of additional hydrophobic amines in mRNA delivery with LNPs and provide valuable insights for the advancement of mRNA delivery carriers.
{"title":"Enhanced mRNA delivery via incorporating hydrophobic amines into lipid nanoparticles","authors":"Longyu Wang , Yichen Li , Pingge Jiang , Hao Bai , Chengfan Wu , Qi Shuai , Yunfeng Yan","doi":"10.1016/j.colsurfb.2025.114528","DOIUrl":"10.1016/j.colsurfb.2025.114528","url":null,"abstract":"<div><div>Lipid nanoparticles (LNPs) have shown promising performance in mRNA delivery. Nevertheless, a thorough understanding of the relationship between mRNA delivery efficacy and the structure of LNPs remains imperative. In this study, we systematically investigated the effects of additional hydrophobic amines on the physicochemical properties of mRNA LNPs and their delivery efficacy. The results indicated that this influence depended on the chemical structure of the additional amines and the structure of the lipid carriers. The appropriate addition of the hydrophobic amine 2C8 to lipid carriers with structural 2C8 or 2C6 tails significantly increased their mRNA delivery efficiency. In contrast, the addition of hydrophobic amine C18 to LNPs resulted in a decrease in mRNA delivery efficiency, while the addition of hydrophobic amines 2C6 and C8, as well as alkanes C12' and C16', had relatively little effect on mRNA delivery. Further investigations demonstrated that the appropriate addition of 2C8 could reduce LNP size, moderate internal hydrophobicity and LNP stability, facilitate mRNA release, enhance cellular uptake, and improve intracellular transportation of LNPs, thereby achieving superior mRNA delivery efficiency. These findings highlight the important role of additional hydrophobic amines in mRNA delivery with LNPs and provide valuable insights for the advancement of mRNA delivery carriers.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"249 ","pages":"Article 114528"},"PeriodicalIF":5.4,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143027488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-20DOI: 10.1016/j.colsurfb.2025.114530
Xiaomei Wu , Zhongyin Chen , Jinyu Wang , Linwei Li , Yuhao Guo , Ziqiang Xu , Ying Kuang , Tao Liao , Cao Li
The integration of photothermal therapy (PTT) and gas therapy (GT) on a nanoplatform shows great potential in cancer treatment. In this paper, a tumor-targeted near-infrared/ultraviolet (NIR/UV) triggered PTT/GT synergistic therapeutic nanoplatform, PB-CD-PLL(NF)-FA, was designed based on Prussian blue (PB) nanoparticles, 5-chloro-2-nitrobenzotrifluoro (NF)-grafted polylysine (PLL(NF)), and folic acid (FA). PB serves as a core to load PLL(NF) through host-guest interaction and can further modify FA. PB-CD-PLL(NF)-FA can be enriched in tumor tissues by passive targeting with enhanced permeability and retention (EPR) effect and active targeting with FA, and can promote the decomposition of NF under UV light irradiation to achieve the precise release of nitric oxide (NO). PB has a good photothermal conversion efficiency in the NIR region and can be used for PTT. The results of in vivo and in vitro studies showed that PB-CD-PLL(NF)-FA has high photothermal conversion efficiency under NIR laser irradiation, and can release NO on demand under UV light irradiation, which shows a good synergistic therapeutic effect of tumor PTT/GT.
{"title":"Tumor-targeted near-infrared/ultraviolet-triggered photothermal/gas therapy nanoplatform for effective cancer synergistic therapy","authors":"Xiaomei Wu , Zhongyin Chen , Jinyu Wang , Linwei Li , Yuhao Guo , Ziqiang Xu , Ying Kuang , Tao Liao , Cao Li","doi":"10.1016/j.colsurfb.2025.114530","DOIUrl":"10.1016/j.colsurfb.2025.114530","url":null,"abstract":"<div><div>The integration of photothermal therapy (PTT) and gas therapy (GT) on a nanoplatform shows great potential in cancer treatment. In this paper, a tumor-targeted near-infrared/ultraviolet (NIR/UV) triggered PTT/GT synergistic therapeutic nanoplatform, PB-CD-PLL(NF)-FA, was designed based on Prussian blue (PB) nanoparticles, 5-chloro-2-nitrobenzotrifluoro (NF)-grafted polylysine (PLL(NF)), and folic acid (FA). PB serves as a core to load PLL(NF) through host-guest interaction and can further modify FA. PB-CD-PLL(NF)-FA can be enriched in tumor tissues by passive targeting with enhanced permeability and retention (EPR) effect and active targeting with FA, and can promote the decomposition of NF under UV light irradiation to achieve the precise release of nitric oxide (NO). PB has a good photothermal conversion efficiency in the NIR region and can be used for PTT. The results of <em>in vivo</em> and <em>in vitro</em> studies showed that PB-CD-PLL(NF)-FA has high photothermal conversion efficiency under NIR laser irradiation, and can release NO on demand under UV light irradiation, which shows a good synergistic therapeutic effect of tumor PTT/GT.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"249 ","pages":"Article 114530"},"PeriodicalIF":5.4,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143035455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study introduces a novel approach to enhance the antibacterial properties of UIO-66 by incorporating both Thymol and ZnO nanoparticles within its framework which represents a significant advancement like exhibiting a synergistic antibacterial effect, providing a prolonged and controlled release, and mitigating cytotoxicity associated with the release of free ZnO nanoparticles by combining these two antimicrobial agents within a single, well-defined metal-organic framework. UIO-66 frameworks are investigated as carriers for the natural antimicrobial agent, Thymol, and ZnONPs offering a novel drug delivery system for antibacterial applications. Results demonstrated 132, 90, 184, and 223 nm sizes for UIO-66, ZnONPs, UIO-66 encapsulated Thymol, and UIO-66 encapsulated both Thymol and ZnONPs, respectively. Successful encapsulation of the antibacterial drug with a high entrapment efficiency of 64 % for Thymol was approved, and 49 % in-vitro release of Thymol was achieved for 72 hours. In-vitro antibacterial assays revealed promising results, with the drug-loaded nanoparticles exhibiting significantly lower MIC values and enhanced bactericidal activity against S. Aureus bacterial strains compared to the free drug, as demonstrated by agar disk diffusion and time-kill assays. MIC values reduced from a range of 31.25–250 µg/ml for free Thymol and 12.5–100 µg/ml for free ZnONPs to 3.9–62.5 µg/ml for Thymol@UIO-66 and 1.95–15.63 µg/ml for Thymol/ZnONPs@UIO-66. According to the results, the mixture of both Thymol and ZnONPs had 41 % and 16 % more antibiofilm activities in comparison with free Thymol and free ZnONPs, respectively. Furthermore, Thymol@UIO-66 had 25 % higher antibiofilm activities relative to not-encapsulated Thymol and ZnONPs, and this improvement was even 46 % more in Thymol/ZnONPs@UIO-66 in comparison with Thymol@UIO-66. Overall, this study demonstrates the potential of Thymol/ZnONPs@UIO-66 frameworks as a promising drug delivery platform for effective antibacterial therapy. This approach to overcome antibiotic resistance and improve treatment efficacy potentially.
{"title":"Antimicrobial and antibiofilm activity of prepared thymol@UIO-66 and thymol/ZnONPs@UIO-66 nanoparticles against Methicillin-resistant Staphylococcus aureus: A synergistic approach","authors":"Alireza Eskandari , Seyedeh Nooshin Safavi , Hamidreza Sahrayi , Dorsa Alizadegan , Mohammadmahdi Eskandarisani , Alireza Javanmard , Mohammadreza Tajik , Zohre Sadeghi , Arvin Toutounch , Faten Eshrati Yeganeh , Hassan Noorbazargan","doi":"10.1016/j.colsurfb.2025.114529","DOIUrl":"10.1016/j.colsurfb.2025.114529","url":null,"abstract":"<div><div>This study introduces a novel approach to enhance the antibacterial properties of UIO-66 by incorporating both Thymol and ZnO nanoparticles within its framework which represents a significant advancement like exhibiting a synergistic antibacterial effect, providing a prolonged and controlled release, and mitigating cytotoxicity associated with the release of free ZnO nanoparticles by combining these two antimicrobial agents within a single, well-defined metal-organic framework. UIO-66 frameworks are investigated as carriers for the natural antimicrobial agent, Thymol, and ZnONPs offering a novel drug delivery system for antibacterial applications. Results demonstrated 132, 90, 184, and 223 nm sizes for UIO-66, ZnONPs, UIO-66 encapsulated Thymol, and UIO-66 encapsulated both Thymol and ZnONPs, respectively. Successful encapsulation of the antibacterial drug with a high entrapment efficiency of 64 % for Thymol was approved, and 49 % in-vitro release of Thymol was achieved for 72 hours. <em>In-vitro</em> antibacterial assays revealed promising results, with the drug-loaded nanoparticles exhibiting significantly lower MIC values and enhanced bactericidal activity against <em>S. Aureus</em> bacterial strains compared to the free drug, as demonstrated by agar disk diffusion and time-kill assays. MIC values reduced from a range of 31.25–250 µg/ml for free Thymol and 12.5–100 µg/ml for free ZnONPs to 3.9–62.5 µg/ml for Thymol@UIO-66 and 1.95–15.63 µg/ml for Thymol/ZnONPs@UIO-66. According to the results, the mixture of both Thymol and ZnONPs had 41 % and 16 % more antibiofilm activities in comparison with free Thymol and free ZnONPs, respectively. Furthermore, Thymol@UIO-66 had 25 % higher antibiofilm activities relative to not-encapsulated Thymol and ZnONPs, and this improvement was even 46 % more in Thymol/ZnONPs@UIO-66 in comparison with Thymol@UIO-66. Overall, this study demonstrates the potential of Thymol/ZnONPs@UIO-66 frameworks as a promising drug delivery platform for effective antibacterial therapy. This approach to overcome antibiotic resistance and improve treatment efficacy potentially.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"249 ","pages":"Article 114529"},"PeriodicalIF":5.4,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143062641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-15DOI: 10.1016/j.colsurfb.2025.114506
Jiang Ni , Yanhua Chen , Lan Zhang , Rong Wang , Xiufeng Wu , Naveed Ullah Khan , Fen Xie
During surgical procedures, skin and soft tissue wounds are often infected by resistant strains of gram-positive bacteria and gram-negative bacteria, resulting in serious obstacles to the healing of these wounds. Commercially available dressings for such wounds are still insufficient to combat resistant infections. Here, we designed vancomycin and epigallocatechin gallate (EGCG) loaded poly(vinyl)-pyrrolidone-gelatine nanofiber’s membrane dressing for potential synergistic efficiency against infected post-surgical wounds. The nanofiber’s membrane was physiochemically characterized by surface morphology, chemical and physical compatibilities’, thermal stability, and drug release. Disk diffusion assays, Minimum inhibitor concentrations (MICs), and fractional inhibitory concentration indexes (FICI) were measured to analyze synergistic efficiency against Escherichia coli. Furthermore, Balb/c mice were used for in vivo healing studies, and to observe the healing mechanisms, histological assessments were performed. The designed system displayed excellent physical and chemical properties. The in vitro studies unveiled controlled-release patterns of vancomycin and EGCG and, at the same time, revealed 1.5-fold higher antimicrobial synergistic efficacy (FICI 0.485) than vancomycin against E. coli. The wound healing mechanisms reflected quick and mature healing processes with the promotion of collagen and angiogenesis at wound sites. The designed electrospun nanofiber technology might be personalized, rapid wound healing remedy for scientists and healthcare providers, and may enhance patients’ outcomes and quality of life.
{"title":"Epigallocatechin gallate and vancomycin loaded poly(vinyl)-pyrrolidone-gelatine nanofibers, conceivable curative approach for wound healing","authors":"Jiang Ni , Yanhua Chen , Lan Zhang , Rong Wang , Xiufeng Wu , Naveed Ullah Khan , Fen Xie","doi":"10.1016/j.colsurfb.2025.114506","DOIUrl":"10.1016/j.colsurfb.2025.114506","url":null,"abstract":"<div><div>During surgical procedures, skin and soft tissue wounds are often infected by resistant strains of gram-positive bacteria and gram-negative bacteria, resulting in serious obstacles to the healing of these wounds. Commercially available dressings for such wounds are still insufficient to combat resistant infections. Here, we designed vancomycin and epigallocatechin gallate (EGCG) loaded poly(vinyl)-pyrrolidone-gelatine nanofiber’s membrane dressing for potential synergistic efficiency against infected post-surgical wounds. The nanofiber’s membrane was physiochemically characterized by surface morphology, chemical and physical compatibilities’, thermal stability, and drug release. Disk diffusion assays, Minimum inhibitor concentrations (MICs), and fractional inhibitory concentration indexes (FICI) were measured to analyze synergistic efficiency against <em>Escherichia coli</em>. Furthermore, Balb/c mice were used for <em>in vivo</em> healing studies, and to observe the healing mechanisms, histological assessments were performed. The designed system displayed excellent physical and chemical properties. The <em>in vitro</em> studies unveiled controlled-release patterns of vancomycin and EGCG and, at the same time, revealed 1.5-fold higher antimicrobial synergistic efficacy (FICI 0.485) than vancomycin against <em>E. coli</em>. The wound healing mechanisms reflected quick and mature healing processes with the promotion of collagen and angiogenesis at wound sites. The designed electrospun nanofiber technology might be personalized, rapid wound healing remedy for scientists and healthcare providers, and may enhance patients’ outcomes and quality of life.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"249 ","pages":"Article 114506"},"PeriodicalIF":5.4,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142997475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-14DOI: 10.1016/j.colsurfb.2025.114520
Xinyun Ye , Huanglian Zhong , Lin Liu , Jingtao Huang , Zhuquan Xia , Zhiji Tang , Wenjin Wei , Weilong Huang , Yuwei Ye , Qiuhua Jiang
To address the medical challenges posed by glioblastoma, a novel and high-performance tumor inhibitor (La@FA-CDs) composed of folic acid and lanthanum nitrate hexahydrate, was successfully synthesized and demonstrated effectiveness in inhibiting the growth of U251 and LN299 cells. The microstructure of La@FA-CDs was extensively analyzed by FTIR, UV-Vis, XPS, TEM, AFM NMR, and nanoparticle size analyzer. The optical and electrical properties of La@FA-CDs were characterized using a fluorescence spectrometer and a zeta potential analyzer. Biological assays, including the CCK8 proliferation assay, scratch assay, flow cytometry, cytoskeleton staining, and live/dead staining were conducted to assess antitumor properties and cytotoxicity. The result revealed that the La50 %@FA-CDs demonstrated significantly enhanced antitumor activity relative to the undoped sample. Furthermore, the La50 %@FA-CDs demonstrated a dose-dependent cytotoxic effect on two glioblastoma cell lines U251 and LN299. The findings of this study suggested that treatment with La50 %@FA-CDs effectively inhibited migration and proliferation while promoting apoptosis in glioblastoma cells. Meanwhile, the La50 %@FA-CDs showed minimal cytotoxic effects on HEK 293 and HUVEC cells under standard conditions, with only slight toxicity observed in HUVEC cells at high (500 µM) concentrations. These results suggest that La50 %@FA-CDs could be a promising therapeutic agent for glioblastoma treatment, demonstrating both effective inhibition and favorable safety profiles.
{"title":"A novel and high-performance tumor inhibitor of La, N co-doped carbon dots for U251 and LN229 cells","authors":"Xinyun Ye , Huanglian Zhong , Lin Liu , Jingtao Huang , Zhuquan Xia , Zhiji Tang , Wenjin Wei , Weilong Huang , Yuwei Ye , Qiuhua Jiang","doi":"10.1016/j.colsurfb.2025.114520","DOIUrl":"10.1016/j.colsurfb.2025.114520","url":null,"abstract":"<div><div>To address the medical challenges posed by glioblastoma, a novel and high-performance tumor inhibitor (La@FA-CDs) composed of folic acid and lanthanum nitrate hexahydrate, was successfully synthesized and demonstrated effectiveness in inhibiting the growth of U251 and LN299 cells. The microstructure of La@FA-CDs was extensively analyzed by FTIR, UV-Vis, XPS, TEM, AFM NMR, and nanoparticle size analyzer. The optical and electrical properties of La@FA-CDs were characterized using a fluorescence spectrometer and a zeta potential analyzer. Biological assays, including the CCK8 proliferation assay, scratch assay, flow cytometry, cytoskeleton staining, and live/dead staining were conducted to assess antitumor properties and cytotoxicity. The result revealed that the La<sub>50 %</sub>@FA-CDs demonstrated significantly enhanced antitumor activity relative to the undoped sample. Furthermore, the La<sub>50 %</sub>@FA-CDs demonstrated a dose-dependent cytotoxic effect on two glioblastoma cell lines U251 and LN299. The findings of this study suggested that treatment with La<sub>50 %</sub>@FA-CDs effectively inhibited migration and proliferation while promoting apoptosis in glioblastoma cells. Meanwhile, the La<sub>50 %</sub>@FA-CDs showed minimal cytotoxic effects on HEK 293 and HUVEC cells under standard conditions, with only slight toxicity observed in HUVEC cells at high (500 µM) concentrations. These results suggest that La<sub>50 %</sub>@FA-CDs could be a promising therapeutic agent for glioblastoma treatment, demonstrating both effective inhibition and favorable safety profiles.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"249 ","pages":"Article 114520"},"PeriodicalIF":5.4,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142997422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-14DOI: 10.1016/j.colsurfb.2025.114509
Zhaozhao Zhang , Fei Gao , Jinlong Mao , Jinjing Liu , Ziyi Zeng , Yukun Zhou , Wenjie Tao , Wenyuan Wang , Gen Lyu , Lei Xu , Guojiang Wan
Neurovascular flow-diverting stents (FDSs) are revolutionizing the paradigm for treatment of intracranial aneurysms, but they still face great challenges like post- implantation acute thrombosis and delayed reendothelialization. Surface modification is of crucial relevance in addressing such key issues. In this study, we fabricated an ultrathin nanocoating out of copper (II) together with protocatechuic acid (PCA) and nattokinase (NK) bioactive molecules on NiTi FDSs via a coordination chemistry approach, with favorable biophysiochemical interactions, to fulfill this goal. This coating was identified as covalently-anchored and compactly covering the FDSs substrate, with unique nano-structured morphology as well as superhydrophilicity. The in vitro coagulation and whole blood assays demonstrated that the modified FDS's surfaces showed improved antithrombogenicity, with reduced platelet and fibrinogen adhesion, as well as their aggregation and activation, and consequently prolonged clotting time leading to decreased thrombosis occurrence. Human umbilical vein endothelial cell cultures confirmed the modified capability of FDSs to promote endothelial cell proliferation and migration. The ex vivo experiments verified that modified FDSs had clearly in-stent patency without thrombi formation, as compared to the bare FDSs bearing thromboembolic blockage. It was postulated that these enhanced biocompatibilities can be attributable to the copper-catalyzed nitric oxide (NO) released as a functional mediator, the nature of the PCA and NK molecules, as well as the synergic biophysiochemical surface/interface interactions. Our strategy may not only open a new avenue for surface-functionalizing neurovascular FDSs for medical purpose but also help better-understand interfacial phenomena on the advanced biomaterials.
{"title":"Biophysiochemically favorable, antithrombotic and pro-endothelial coordination compound nanocoating of copper (II) with protocatechuic acid & nattokinase on flow-diverting stents","authors":"Zhaozhao Zhang , Fei Gao , Jinlong Mao , Jinjing Liu , Ziyi Zeng , Yukun Zhou , Wenjie Tao , Wenyuan Wang , Gen Lyu , Lei Xu , Guojiang Wan","doi":"10.1016/j.colsurfb.2025.114509","DOIUrl":"10.1016/j.colsurfb.2025.114509","url":null,"abstract":"<div><div>Neurovascular flow-diverting stents (FDSs) are revolutionizing the paradigm for treatment of intracranial aneurysms, but they still face great challenges like post- implantation acute thrombosis and delayed reendothelialization. Surface modification is of crucial relevance in addressing such key issues. In this study, we fabricated an ultrathin nanocoating out of copper (II) together with protocatechuic acid (PCA) and nattokinase (NK) bioactive molecules on NiTi FDSs via a coordination chemistry approach, with favorable biophysiochemical interactions, to fulfill this goal. This coating was identified as covalently-anchored and compactly covering the FDSs substrate, with unique nano-structured morphology as well as superhydrophilicity. The <em>in vitro</em> coagulation and whole blood assays demonstrated that the modified FDS's surfaces showed improved antithrombogenicity, with reduced platelet and fibrinogen adhesion, as well as their aggregation and activation, and consequently prolonged clotting time leading to decreased thrombosis occurrence. Human umbilical vein endothelial cell cultures confirmed the modified capability of FDSs to promote endothelial cell proliferation and migration. The <em>ex vivo</em> experiments verified that modified FDSs had clearly in-stent patency without thrombi formation, as compared to the bare FDSs bearing thromboembolic blockage. It was postulated that these enhanced biocompatibilities can be attributable to the copper-catalyzed nitric oxide (NO) released as a functional mediator, the nature of the PCA and NK molecules, as well as the synergic biophysiochemical surface/interface interactions. Our strategy may not only open a new avenue for surface-functionalizing neurovascular FDSs for medical purpose but also help better-understand interfacial phenomena on the advanced biomaterials.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"249 ","pages":"Article 114509"},"PeriodicalIF":5.4,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142997436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abdominal adhesions are a frequent complication after abdominal surgery, which can cause significant pain and burden to patients. Despite various treatment options, including surgical intervention and pharmacotherapy, these often fail to consistently and effectively prevent postoperative abdominal adhesions. Janus hydrogel is famous for its asymmetric characteristics, which shows great prospects in the prevention and treatment of abdominal adhesion. This review outlines the preparation methods, mechanisms of action, and key applications of Janus hydrogel in the prevention of postoperative abdominal adhesions. Furthermore, we examine the current limitations of the Janus hydrogel anti-adhesion barrier and explore potential future directions for its development.
{"title":"A novel strategy for addressing post-surgical abdominal adhesions: Janus hydrogel","authors":"Xinhui Zhu , Lipeng Zhang , Yingcheng Qi , Jingyu Zhang , Fuxin Tang , Zhen Zong","doi":"10.1016/j.colsurfb.2025.114511","DOIUrl":"10.1016/j.colsurfb.2025.114511","url":null,"abstract":"<div><div>Abdominal adhesions are a frequent complication after abdominal surgery, which can cause significant pain and burden to patients. Despite various treatment options, including surgical intervention and pharmacotherapy, these often fail to consistently and effectively prevent postoperative abdominal adhesions. Janus hydrogel is famous for its asymmetric characteristics, which shows great prospects in the prevention and treatment of abdominal adhesion. This review outlines the preparation methods, mechanisms of action, and key applications of Janus hydrogel in the prevention of postoperative abdominal adhesions. Furthermore, we examine the current limitations of the Janus hydrogel anti-adhesion barrier and explore potential future directions for its development.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"249 ","pages":"Article 114511"},"PeriodicalIF":5.4,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142997429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Electrical stimulation displayed tremendous potential in promoting nerve regeneration. However, the current electrical stimulation therapy required complex traversing wires and external power sources, which significantly limited its practical application. Herein, a self-powered nerve scaffold based on primary battery principle was gradient printed by laser additive manufacturing technique. Specifically, poly-L-lactide (PLLA) containing Ag2O and Zn nanoparticles was prepared as the positive and negative electrode of the scaffold respectively, and PLLA/PPy was prepared as the middle conductive segment. In simulated body fluid, the negative electrode underwent oxidation to lose electrons and become positively charged. The lost electrons were transferred to the positive segment in a directed and orderly manner via the middle conductive segment, causing the positive electrode to be enriched electrons and become negatively charged. Subsequently, two segments can generate a potential difference to form an electric field, further generating current. Not merely, the redox process can release Ag+ and Zn2+ to endow the scaffold with antibacterial properties. Results showed that the scaffold could generate a current of up to 17.2 μA, which promoted a 14-fold increase in calcium ion influx and increased the mRNA expression of neuronal markers MAP2 by 24-fold. Moreover, the antibacterial rates of the scaffold against E. coli and S. aureus could reach 92.6 % and 91.9 %, respectively.
{"title":"Engineering a wirelessly self-powered neural scaffold based on primary battery principle to accelerate nerve cell differentiation","authors":"Huixing Li , Xiong Shuai , Yanyan Chen , Jiaxing Xiong , Zhongxing Zou , Shuping Peng , Fangwei Qi , Cijun Shuai","doi":"10.1016/j.colsurfb.2025.114521","DOIUrl":"10.1016/j.colsurfb.2025.114521","url":null,"abstract":"<div><div>Electrical stimulation displayed tremendous potential in promoting nerve regeneration. However, the current electrical stimulation therapy required complex traversing wires and external power sources, which significantly limited its practical application. Herein, a self-powered nerve scaffold based on primary battery principle was gradient printed by laser additive manufacturing technique. Specifically, poly-L-lactide (PLLA) containing Ag<sub>2</sub>O and Zn nanoparticles was prepared as the positive and negative electrode of the scaffold respectively, and PLLA/PPy was prepared as the middle conductive segment. In simulated body fluid, the negative electrode underwent oxidation to lose electrons and become positively charged. The lost electrons were transferred to the positive segment in a directed and orderly manner via the middle conductive segment, causing the positive electrode to be enriched electrons and become negatively charged. Subsequently, two segments can generate a potential difference to form an electric field, further generating current. Not merely, the redox process can release Ag<sup>+</sup> and Zn<sup>2+</sup> to endow the scaffold with antibacterial properties. Results showed that the scaffold could generate a current of up to 17.2 μA, which promoted a 14-fold increase in calcium ion influx and increased the mRNA expression of neuronal markers MAP2 by 24-fold. Moreover, the antibacterial rates of the scaffold against <em>E. coli</em> and <em>S. aureus</em> could reach 92.6 % and 91.9 %, respectively.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"249 ","pages":"Article 114521"},"PeriodicalIF":5.4,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142997390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-13DOI: 10.1016/j.colsurfb.2025.114512
Siyu Jia , Yaohui Chen , Can Zhuo , Ming Hu , Chengwei Zhang , Huili Cai , Xinzhi Li , Haidan Chen , Xiang Yu
Osteosarcoma (OS) is the most common primary bone malignancy characterized by deposition of an immature osteoid matrix. OS treatment has proven challenging because of the high risk of metastatic progression and recurrence after chemotherapy. Melittin (MLT) is recognized as a potential antitumor candidate to overcome chemotherapy resistance and provoke superior immunostimulatory effects. However, the application of MLT to OS is hampered by severe toxic side effects and a lack of tumor-targeting ability. Herein, a self-assembled nanopolymer named LC09-MLT@F127 was developed by binding MLT with F127 micelles and then modifying an aptamer (LC09) for targeted drug delivery during OS treatment. LC09-MLT@F127 exhibited significant OS-targeting ability in vitro and in vivo owing to the aptamer LC09 decoration. Moreover, LC09-MLT@F127 significantly reduced the hemolytic toxicity of MLT while maintaining its tumor-killing ability. In an orthotopic transplantation model of OS, LC09-MLT@F127 induced immunogenic cell death and facilitated the maturation of dendritic cells (DCs), thereby resulting in the activation of tumor-specific immune responses and the inhibition of OS deterioration. Taken together, these finding suggest that LC09-MLT@F127 may be an encouraging MLT-based immunotherapy option for OS.
{"title":"Aptamer-modified melittin micelles efficiently inhibit osteosarcoma deterioration by inducing immunogenic cell death","authors":"Siyu Jia , Yaohui Chen , Can Zhuo , Ming Hu , Chengwei Zhang , Huili Cai , Xinzhi Li , Haidan Chen , Xiang Yu","doi":"10.1016/j.colsurfb.2025.114512","DOIUrl":"10.1016/j.colsurfb.2025.114512","url":null,"abstract":"<div><div>Osteosarcoma (OS) is the most common primary bone malignancy characterized by deposition of an immature osteoid matrix. OS treatment has proven challenging because of the high risk of metastatic progression and recurrence after chemotherapy. Melittin (MLT) is recognized as a potential antitumor candidate to overcome chemotherapy resistance and provoke superior immunostimulatory effects. However, the application of MLT to OS is hampered by severe toxic side effects and a lack of tumor-targeting ability. Herein, a self-assembled nanopolymer named LC09-MLT@F127 was developed by binding MLT with F127 micelles and then modifying an aptamer (LC09) for targeted drug delivery during OS treatment. LC09-MLT@F127 exhibited significant OS-targeting ability <em>in vitro</em> and <em>in vivo</em> owing to the aptamer LC09 decoration. Moreover, LC09-MLT@F127 significantly reduced the hemolytic toxicity of MLT while maintaining its tumor-killing ability. In an orthotopic transplantation model of OS, LC09-MLT@F127 induced immunogenic cell death and facilitated the maturation of dendritic cells (DCs), thereby resulting in the activation of tumor-specific immune responses and the inhibition of OS deterioration. Taken together, these finding suggest that LC09-MLT@F127 may be an encouraging MLT-based immunotherapy option for OS.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"249 ","pages":"Article 114512"},"PeriodicalIF":5.4,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143021430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-12DOI: 10.1016/j.colsurfb.2025.114510
Lu Han , Xiaoyun Zhang , Fei Wu , Tianhua Wang , Honglin Zhai
Since defects in nanomaterials are inevitable during experimental manipulation, investigating the interactions between defective materials and active biological proteins is crucial for evaluating the biocompatibility and biosafety of nanomaterials. This study employs molecular dynamics simulation techniques to investigate the interaction mechanisms between two types of graphene (ideal graphene and defective graphene) and two model proteins (BBA protein and λ-repressor protein). The simulation results indicate that both types of graphene exhibit superior biocompatibility with the λ-repressor protein compared to the BBA protein. The difference in binding modes of the BBA protein with the two graphenes arises mainly from its initial orientation. Notably, the positively charged Arg residue forces the BBA protein to "anchor" to the surface of defective graphene, significantly restricting its lateral migration. The λ-repressor protein is "anchored" onto the surface of defective graphene through hydrogen bonding interactions involving its Ser residue. Such hydrogen bonding was never reported in similar systems. The distinctive binding modes of these two model proteins with defective graphene are beneficial for the future development of safer and more efficient nanomedicine technologies.
{"title":"Exploring the binding mode of BBA protein anchored on defective graphene and evaluating the biocompatibility of two types of graphene with λ-repressor protein","authors":"Lu Han , Xiaoyun Zhang , Fei Wu , Tianhua Wang , Honglin Zhai","doi":"10.1016/j.colsurfb.2025.114510","DOIUrl":"10.1016/j.colsurfb.2025.114510","url":null,"abstract":"<div><div>Since defects in nanomaterials are inevitable during experimental manipulation, investigating the interactions between defective materials and active biological proteins is crucial for evaluating the biocompatibility and biosafety of nanomaterials. This study employs molecular dynamics simulation techniques to investigate the interaction mechanisms between two types of graphene (ideal graphene and defective graphene) and two model proteins (BBA protein and λ-repressor protein). The simulation results indicate that both types of graphene exhibit superior biocompatibility with the λ-repressor protein compared to the BBA protein. The difference in binding modes of the BBA protein with the two graphenes arises mainly from its initial orientation. Notably, the positively charged Arg residue forces the BBA protein to \"anchor\" to the surface of defective graphene, significantly restricting its lateral migration. The λ-repressor protein is \"anchored\" onto the surface of defective graphene through hydrogen bonding interactions involving its Ser residue. Such hydrogen bonding was never reported in similar systems. The distinctive binding modes of these two model proteins with defective graphene are beneficial for the future development of safer and more efficient nanomedicine technologies.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"249 ","pages":"Article 114510"},"PeriodicalIF":5.4,"publicationDate":"2025-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142997489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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