Colloids and Surfaces B: Biointerfaces最新文献_第7页

A host-guest mediated immunomodulatory hydrogel with sustained antibacterial activity for healing of infected diabetic wounds 一种主客体介导的免疫调节水凝胶，具有持续的抗菌活性，可用于糖尿病感染伤口的愈合

IF 5.6 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2025-11-21 DOI: 10.1016/j.colsurfb.2025.115305

Chunxian Wang , Pingting Liu , Yang Liu, Xinni Shen, Yujia Wu, Pengyu Wang

To solve the hinderance of infected diabetic wound regeneration and healing, an antibacterial and immunomodulatory hydrogel based on host-guest mediated was designed and prepared. Firstly, carboxymethyl chitosan-carboxymethyl cyclodextrin (CMCS-CMCD) was synthesized by chemical grafting, which was applied to load essential oils for sustained active functions. Based on the host-guest mediated effect, six out of fifteen essential oils, most of them containing phenyl groups, were screened with better antibacterial effect by molecular docking. Subsequently, clove essential oil (CEO) was proved experimentally with optimal antibacterial effect and gelation properties. Secondly, sodium alginate-dopamine hydrochloride (SA-DH) was chemically modified to improve adhesion. Then the multifunctional hydrogel (SD-SD/CEO) was further constructed by physical crosslinking with CMCS-CMCD/CEO and SA-DH, in which the grafted DH could enhance almost 6-fold of the hydrogel adhesion. The bacterial inhibition experiments of SD-SD/CEO hydrogel displayed the slow release of CEO due to the host-guest mediated effect between CMCS-CMCD and CEO, which could effectively prolong the bacterial inhibition time. The bacterial inhibition experiments of SD-SD/CEO hydrogel displayed the slow release of CEO due to the host-guest mediated effect between CMCS-CMCD and CEO, which could effectively prolong the bacterial inhibition time. Finally, SD-SD/CEO hydrogel treated infected diabetic wound in vivo. The result proved that the multifunctional hydrogel has sustained antibacterial effect during wound repair, by promoting collagen deposition and facilitating immunomodulation in wound repair.

为解决糖尿病感染创面再生愈合的障碍，设计并制备了一种基于主客体介导的抗菌免疫调节水凝胶。首先，通过化学接枝法制备了羧甲基壳聚糖-羧甲基环糊精（CMCS-CMCD），并将其应用于负载精油以获得持续的活性功能。基于主客体介导效应，通过分子对接筛选出15种精油中6种具有较好的抑菌效果，其中大部分含有苯基。随后，丁香精油（CEO）被实验证明具有最佳的抗菌效果和凝胶性。其次，对海藻酸钠-多巴胺盐酸盐（SA-DH）进行化学修饰，提高黏附性。然后将CMCS-CMCD/CEO和SA-DH进行物理交联构建多功能水凝胶（SD-SD/CEO），其中接枝的DH可以使水凝胶的粘附力提高近6倍。SD-SD/CEO水凝胶的抑菌实验表明，由于CMCS-CMCD与CEO之间的主客体介导作用，CEO的释放速度较慢，可以有效延长细菌的抑制时间。SD-SD/CEO水凝胶的抑菌实验表明，由于CMCS-CMCD与CEO之间的主客体介导作用，CEO的释放速度较慢，可以有效延长细菌的抑制时间。最后，SD-SD/CEO水凝胶在体内治疗糖尿病感染创面。结果证明，该多功能水凝胶在伤口修复过程中具有持续的抗菌作用，其机制是促进伤口修复过程中胶原沉积，促进免疫调节。

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引用次数: 0

Anisotropic growth method for constructing stimulus-responsive janus composite nanoparticles for dual-drug delivery in tumor therapy 构建刺激-反应janus复合纳米颗粒的各向异性生长方法用于肿瘤治疗双药递送

IF 5.6 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2025-11-21 DOI: 10.1016/j.colsurfb.2025.115301

Yifan Peng , Xiaoqin Cai , Shanshan Wang , Kaikun Pi , Bin Sun , Yan Li , Bing Liu , Fangxiang Song

In this study, we used an interfacial energy-mediated, anisotropic growth method to synthesize rod-shaped, periodic mesoporous organosilica (PMO) on the surface of the mesoporous silicon-titanium composite nanoparticles (MSTNs) with a core-shell structure, resulting in sphere-rod structured, Janus composite nanoparticles (MSTNs/PMO). Adjusting the amount of surfactant and the solvent ratio (water/ethanol) in the reaction system allowed for the fabrication of MSTNs/PMO nanoparticles with diverse morphologies, and their growth mechanism was analyzed systematically. The MSTNs/PMO surface was functionalized with amino and thiol groups to facilitate the loading of doxorubicin (DOX) and tetrandrine (TET). Then, folic acid (FA) was grafted onto the drug-loaded nanoparticles (MSTNs/PMO-DOX/TET-FA). This step successfully constructed a stimulus-responsive combinatorial dual-drug delivery system. MSTNs/PMO-DOX/TET-FA exhibited high loading efficiencies, reaching 26.54 % for DOX and 6.37 % for TET. Drug release studies demonstrated significant pH-responsive release behavior and sequential release characteristics of the system. Cytotoxicity and cellular uptake assays revealed that the system exhibited excellent biosafety and significant targeting ability toward tumor cells. Collectively, these findings suggested that the dual-drug delivery system has potent antitumor therapeutic efficacy and offered a viable strategy for synergistic tumor therapy.

在本研究中，我们采用界面能介导的各向异性生长方法，在具有核-壳结构的介孔硅-钛复合纳米颗粒（MSTNs）表面合成了棒状、周期性介孔有机二氧化硅（PMO），得到了球-棒结构的Janus复合纳米颗粒（MSTNs/PMO）。通过调节反应体系中表面活性剂的用量和溶剂（水/乙醇）的比例，制备出形貌各异的MSTNs/PMO纳米颗粒，并对其生长机理进行了系统分析。MSTNs/PMO表面被氨基和巯基功能化，以促进阿霉素（DOX）和粉防己碱（TET）的负载。然后，将叶酸（FA）接枝到载药纳米颗粒（MSTNs/PMO-DOX/TET-FA）上。这一步成功构建了刺激-反应组合双药给药系统。MSTNs/PMO-DOX/TET- fa表现出较高的负载效率，DOX和TET的负载效率分别达到26.54 %和6.37 %。药物释放研究表明，该系统具有显著的ph响应释放行为和顺序释放特性。细胞毒性和细胞摄取实验表明，该系统具有良好的生物安全性和对肿瘤细胞的靶向能力。综上所述，这些发现表明，双药传递系统具有强大的抗肿瘤治疗效果，为肿瘤协同治疗提供了一种可行的策略。

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引用次数: 0

Microneedles-photothermal therapy synergy in biomedicine: Innovations and applications 微针-光热疗法在生物医学中的协同作用：创新与应用

IF 5.6 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2025-11-21 DOI: 10.1016/j.colsurfb.2025.115303

Dan He , Tian Liang , Yongjin Zhong , Keng-Fu Lan , Siqi Ye , Yingying Wu , Anchun Mo

Microneedles (MNs) have emerged as a minimally invasive transdermal platform enabling precise, localized drug delivery with enhanced patient compliance. Coupling MNs with photothermal therapy (PTT) integrates spatially targeted delivery and near infrared-triggered thermal ablation, offering synergistic advantages in tumor treatment, wound healing, and the management of various diseases. This review systematically examines the design strategies of MNs-PTT systems and their applications in different diseases. In tumor therapy, advanced structures and multimodal approaches such as combining PTT with chemotherapy, chemodynamic therapy, immunotherapy and gas therapy, address the limitations of conventional treatments. In wound management, biomimetic architectures and microenvironment-responsive materials enable real-time monitoring and adaptive drug release. For other diseases, including inflammatory skin disorders, ocular diseases, oral diseases, autoimmune conditions, metabolic disorders, and other tissue injuries, MNs-PTT systems provide controlled, on-demand interventions tailored to the specific pathology, thereby improving therapeutic efficacy while reducing side effects. Unlike previous reviews that treat these domains separately, this work provides an integrated cross-domain analysis connecting material and structural innovations to therapeutic performance, thus establishing a unified framework for advancing MNs-PTT systems. Despite these advances, challenges regarding clinical translation still remain. Future progress will rely on interdisciplinary efforts to improve biocompatibility, optimize mechanical performance, and integrate multi-stimuli responsiveness. The combination of AI-assisted design, wearable biosensors, and 3D printing is expected to enhance system precision, safety, and adaptability, accelerating the development of personalized therapies.

微针（MNs）已经成为一种微创透皮平台，能够精确、局部地给药，提高患者的依从性。MNs与光热疗法（PTT）的耦合整合了空间靶向递送和近红外触发的热消融，在肿瘤治疗、伤口愈合和各种疾病的治疗方面具有协同优势。本文综述了MNs-PTT系统的设计策略及其在不同疾病中的应用。在肿瘤治疗中，先进的结构和多模式的方法，如PTT联合化疗、化疗动力学治疗、免疫治疗和气体治疗，解决了传统治疗的局限性。在伤口管理中，仿生结构和微环境响应材料能够实时监测和自适应药物释放。对于其他疾病，包括炎症性皮肤病、眼部疾病、口腔疾病、自身免疫性疾病、代谢紊乱和其他组织损伤，MNs-PTT系统提供针对特定病理的受控、按需干预措施，从而提高治疗效果，同时减少副作用。与之前单独处理这些领域的综述不同，这项工作提供了一个集成的跨领域分析，将材料和结构创新与治疗性能联系起来，从而为推进MNs-PTT系统建立了统一的框架。尽管取得了这些进展，但临床翻译方面的挑战仍然存在。未来的进展将依赖于跨学科的努力，以提高生物相容性，优化机械性能，并整合多刺激反应性。人工智能辅助设计、可穿戴生物传感器和3D打印的结合有望提高系统的精度、安全性和适应性，加速个性化治疗的发展。

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引用次数: 0

Sulfonated chitosan combined with exosomes synergistically promotes vascularization and macrophage M2 polarization to promote wound healing 磺化壳聚糖联合外泌体协同促进血管化和巨噬细胞M2极化，促进创面愈合

IF 5.6 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2025-11-21 DOI: 10.1016/j.colsurfb.2025.115306

Yujia Zhai , Bin Liu , Zhenling Zhao , Jie Zou , Yan Mai , Zumin Xu , Xiang Li

Effective wound management remains a critical clinical challenge, with its inherent complexity posing a substantial burden on patient quality of life. Inadequate treatment can result in severe complications, including tissue infection, necrosis, and the loss of both localized and systemic physiological functions. Consequently, over the past decade, researchers have actively explored new approaches to accelerate wound healing. Here, we report the design of a multifunctional composite hydrogel dressing that simultaneously suppresses inflammation and promotes tissue regeneration by enhancing angiogenesis and modulating macrophage polarization. The hydrogel is crosslinked from amino-functionalized hyaluronic acid, aldehyde-functionalized dextran, and sulfonated chitosan, exhibiting excellent mechanical properties and biocompatibility, rendering it a highly suitable platform for controlled and sustained therapeutic delivery. Specifically, the hydrogel is engineered to incorporate exosomes derived from human umbilical cord mesenchymal stem cells (HUCMSC-Exo), which encapsulate hexaminolevulinate hydrochloride (HAL). HAL@Exo regulates cell communication, enhances cellular migration and proliferation, facilitates collagen matrix deposition and angiogenesis, and produces anti-inflammatory bioactive substances through biometabolic processes, thereby alleviating the extent of local inflammatory response. In a rat full-thickness skin wound model, the hydrogel demonstrated a pronounced therapeutic effect, significantly accelerating the wound healing process.

有效的伤口管理仍然是一个关键的临床挑战，其固有的复杂性对患者的生活质量造成了巨大的负担。治疗不当可导致严重的并发症，包括组织感染、坏死以及局部和全身生理功能的丧失。因此，在过去的十年中，研究人员积极探索加速伤口愈合的新方法。在这里，我们报道了一种多功能复合水凝胶敷料的设计，该敷料通过增强血管生成和调节巨噬细胞极化来同时抑制炎症和促进组织再生。该水凝胶由氨基功能化透明质酸、醛功能化葡聚糖和磺化壳聚糖交联而成，具有优异的机械性能和生物相容性，是一种非常合适的可控和持续治疗递送平台。具体来说，水凝胶被设计成包含来自人脐带间充质干细胞（HUCMSC-Exo）的外泌体，该外泌体包封盐酸六聚乙酰丙酸盐（HAL）。HAL@Exo调节细胞通讯，促进细胞迁移和增殖，促进胶原基质沉积和血管生成，并通过生物代谢过程产生抗炎生物活性物质，从而减轻局部炎症反应的程度。在大鼠全层皮肤创面模型中，水凝胶显示出明显的治疗效果，显著加速创面愈合过程。

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引用次数: 0

Dynamic interplay between lateral diffusion and conformational states in a secondary transporter revealed by high-speed AFM 高速AFM揭示的二级转运体横向扩散与构象态之间的动态相互作用

IF 5.6 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2025-11-21 DOI: 10.1016/j.colsurfb.2025.115282

Òscar Domènech , Xuan Kien Ngo , Adrià Botet-Carreras , Jordi H. Borrell

Understanding how membrane proteins coordinate their structural dynamics with their local lipid environment is a crucial process for their function and potential nanotechnological applications. Here, we apply high-speed atomic force microscopy (HS-AFM) to directly visualize, in real time, the lateral motion and substrate-induced conformational changes of the lactose permease (LacY) reconstituted into proteolipid sheets. We observe that LacY dimers diffuse laterally with an average velocity of ∼3 nm s⁻¹ and a diffusion coefficient of ∼18 nm² s⁻¹, suggesting that proteins can relocate tens of nanometers before each transport cycle. The addition of lactose and ionophores (valinomycin, nigericin) triggers distinct nanomechanical and morphological responses, revealing a tight coupling between electrochemical gradients, membrane mechanics, and the conformational state of LacY. These findings support the view that transport efficiency is not solely governed by static structure but is dynamically modulated by the protein’s lateral mobility and mechanical feedback from the membrane. Our results offer a mechanistic framework for studying secondary transporters in native-like environments and highlight the potential of HS-AFM to resolve nanoscale dynamics in complex membrane systems.

了解膜蛋白如何与其局部脂质环境协调其结构动力学是其功能和潜在纳米技术应用的关键过程。在这里，我们使用高速原子力显微镜（HS-AFM）直接实时观察乳糖渗透酶（LacY）重组成蛋白脂片的横向运动和底物诱导的构象变化。我们观察到LacY二聚体横向扩散的平均速度为~ 3 nm s - 1，扩散系数为~ 18 nm²s - 1，这表明蛋白质在每次运输循环之前可以重新定位几十纳米。乳糖和离子载体（valinomycin, nigericin）的加入引发了不同的纳米力学和形态反应，揭示了电化学梯度、膜力学和LacY构象状态之间的紧密耦合。这些发现支持了这样一种观点，即转运效率不仅受静态结构的控制，而且受蛋白质的横向迁移和来自膜的机械反馈的动态调节。我们的研究结果为研究原生环境中的二次转运蛋白提供了一个机制框架，并强调了HS-AFM在复杂膜系统中解决纳米级动力学问题的潜力。

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引用次数: 0

Corrigendum to “A CuS-loaded copper-ferrocene framework with synergistic chemodynamic and photothermal antimicrobial therapy for accelerated infected wound healing” [Colloids Surf. B Biointerfaces 258 (2026) 115239] “负载cu的铜-二茂铁框架与协同化学动力学和光热抗菌治疗加速感染伤口愈合”的更正[Colloids Surf]。[2]中国生物医学工程学报[j]。

IF 5.6 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2025-11-20 DOI: 10.1016/j.colsurfb.2025.115291

Yi-Fei Hui , Jilin Jiang , Yi-Han Lin , Zijie Zhou , Duan-Ping Hu , Lin-Yi Li , Fa-Qiang Tang , Zu-Jin Lin , Lai-Peng Yan

引用次数: 0

A pH-sensitive smart hydrogel based on oxidized hyaluronic acid with synergistic antibacterial properties 一种基于氧化透明质酸的ph敏感智能水凝胶，具有协同抗菌性能

IF 5.6 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2025-11-20 DOI: 10.1016/j.colsurfb.2025.115295

Zimin Wan , Yifan Zhao , Xueqiong Zhang , Dan Li , Hua Zheng

Wound infections represent a prevalent clinical challenge. Bacterial infections can impede wound healing, potentially resulting in the development of chronic wounds that pose life-threatening risks in severe cases. In this study, a pH-responsive smart hydrogel for the co-delivery of tobramycin (TOB) and the antibiotic adjuvant berberine (BBR) was designed, aiming to reduce side effects and enhance the antibacterial activity of antibiotics. In the simulated inflammatory environment with a pH of 5.0, the release percentages for TOB and BBR after 48 h were 72.12 % and 93.11 %, respectively. Meanwhile, the hydrogel shows marvelous swelling performance, injectability, good biocompatibility, and excellent self-healing characteristics. The antibacterial assays show that BBR potentiated the antibacterial activity of antibiotics, and the synergistic effect of the dual-drug greatly eradicates the majority of bacteria. These findings collectively suggest that pH-responsive smart hydrogels represent a promising strategy for antibiotic delivery.

伤口感染是一个普遍的临床挑战。细菌感染会阻碍伤口愈合，可能导致慢性伤口的发展，严重时可能危及生命。本研究设计了一种ph响应型智能水凝胶，用于妥布霉素（tobramycin， TOB）和抗生素佐剂小檗碱（berberine， BBR）的共递送，旨在减少抗生素的副作用，增强抗生素的抗菌活性。在pH为5.0的模拟炎症环境中，48 h后TOB和BBR的释放率分别为72.12 %和93.11 %。同时，该水凝胶具有良好的溶胀性、注射性、生物相容性和良好的自愈性。抑菌试验表明，BBR可增强抗生素的抑菌活性，双药协同作用可杀灭大部分细菌。这些发现共同表明，ph响应智能水凝胶代表了一种有前途的抗生素递送策略。

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引用次数: 0

Madecassoside-functionalized platinum-based liposomes for sensitive skin: Enhancing rapid soothing and barrier homeostasis 为敏感皮肤制备的茄苷功能化铂基脂质体：增强快速舒缓和屏障稳态

IF 5.6 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2025-11-20 DOI: 10.1016/j.colsurfb.2025.115299

Jing Sun , Dongying Zhang , Dan Wang , Shining Li , Yongjie Lu , Lu Ren , Shuangyan Wang , Yanhong Liu , Zhiting Zhang , Sumei Han , Haixiao Wan , Ying Wu , Qipeng Lu , Yan Huang , Na Li , Tomoko Oto , Li Ye , Dongcui Li , Naisheng Jiang

Platinum-based materials exhibit diverse biological activities, but their application in skincare is limited by poor targeting and low efficacy in treating inflammation and sensory irritation. Herein, we developed madecassoside-functionalized platinum (Pt-MAD) particles by integrating the bioactive plant-derived compound madecassoside (MAD) with platinum to enhance anti-inflammatory and neurogenic-soothing effects. These Pt-MAD complexes were encapsulated within liposomes to improve dispersion stability and transdermal delivery for dermatological applications. In vitro studies demonstrated that Pt-MAD liposomes outperformed conventional Pt-PVP liposomes in reducing inflammation, itch, and redness. MAD surface functionalization enabled targeted modulation of multiple inflammatory mediators, including TNF-α, IL-1β, and IL-17, thereby promoting rapid soothing while supporting epidermal barrier homeostasis through sustained regulation of neurogenic inflammatory pathways. To explore the underlying mechanism, AI-driven docking simulations were performed, revealing that Pt-MAD exhibited strong binding affinities toward key inflammatory receptors, including TRPV1, TRPA1, NK1R, H1R, ETAR. These findings highlight the therapeutic potential of Pt-MAD liposomes as a targeted and effective strategy for managing sensitive skin, offering rapid relief while supporting barrier homeostasis through IL-17-associated inflammatory modulation.

铂基材料具有多种生物活性，但在治疗炎症和感觉刺激方面靶向性差、疗效低，限制了其在护肤领域的应用。在此，我们通过整合生物活性植物源化合物madasas皂苷（MAD）与铂，开发了madasas皂苷功能化铂（Pt-MAD）颗粒，以增强抗炎和神经源性舒缓作用。这些Pt-MAD复合物被封装在脂质体中，以提高分散稳定性和皮肤应用的透皮递送。体外研究表明，Pt-MAD脂质体在减轻炎症、瘙痒和发红方面优于传统的Pt-PVP脂质体。MAD表面功能化能够靶向调节多种炎症介质，包括TNF-α、IL-1β和IL-17，从而促进快速舒缓，同时通过持续调节神经源性炎症途径支持表皮屏障稳态。为了探索其潜在机制，进行了人工智能驱动的对接模拟，结果显示Pt-MAD对关键炎症受体（包括TRPV1， TRPA1, NK1R, H1R， ETAR）具有很强的结合亲和力。这些发现强调了Pt-MAD脂质体作为治疗敏感皮肤的一种有针对性和有效的策略的治疗潜力，通过il -17相关的炎症调节提供快速缓解，同时支持屏障稳态。

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引用次数: 0

A multifunctional nanosystem based on sodium nitroprusside/copper co-doped dopamine-coated Prussian blue nanoparticles with enhanced photothermal capability for combined cancer therapy 一种基于硝普钠/铜共掺杂多巴胺涂层普鲁士蓝纳米粒子的多功能纳米系统，具有增强的光热能力，用于联合癌症治疗

IF 5.6 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2025-11-20 DOI: 10.1016/j.colsurfb.2025.115298

Tao Liao , Jiahao Chen , Linwei Li , Jinyu Wang , Siyu Zhang , Jia Liu , Ying Kuang , Cao Li

Cancer remains a significant global health challenge requiring advanced therapeutic strategies to minimize off-target effects. In this work, we report a multifunctional nanoplatform (DOX@PB-NO@PDA-Cu), which combines photothermal therapy (PTT), chemotherapy, gas therapy, and chemodynamic therapy (CDT) to achieve synergistic tumor ablation. The system is engineered by doping sodium nitroprusside (SNP) into Prussian blue nanoparticles (PB NPs), enabling the release of nitric oxide (NO). Then, the NPs are coated with polydopamine (PDA) and undergo Cu^2 + -coordination. SNP doping and subsequent Cu/PDA modification significantly red-shift the near-infrared (NIR) absorption, substantially enhancing photothermal conversion at 808 nm. The PDA shell acts as a “gatekeeper” for pH- and thermal-responsive doxorubicin (DOX) release, enabling NO liberation under photothermal activation. Cu²⁺ depletes intracellular glutathione and catalyzes Fenton-like reactions to generate hydroxyl radicals (•OH), thereby amplifying oxidative stress. In vitro studies confirm efficient cellular uptake, on-demand NO and reactive oxygen species (ROS) generation, and synergistic cytotoxicity. In vivo evaluations demonstrate significant tumor suppression via combined PTT/chemotherapy/gas therapy/CDT, with minimal systemic toxicity, as validated by histopathology and blood biochemistry. This design exemplifies a precision nanomedicine strategy that uses endogenous stimuli and exogenous activation to effectively treat cancer combinatorially.

癌症仍然是一个重大的全球健康挑战，需要先进的治疗策略来尽量减少脱靶效应。在这项工作中，我们报道了一个多功能纳米平台（DOX@PB-NO@PDA-Cu），它结合了光热治疗（PTT）、化疗、气体治疗和化学动力治疗（CDT）来实现协同肿瘤消融。该系统是通过将硝普钠（SNP）掺杂到普鲁士蓝纳米颗粒（PB NPs）中，从而释放一氧化氮（NO）而设计的。然后，NPs被聚多巴胺（PDA）包裹，并进行Cu2 +配位。SNP掺杂和随后的Cu/PDA修饰显著红移近红外（NIR）吸收，显著增强808 nm的光热转换。PDA外壳充当pH和热响应性阿霉素（DOX）释放的“看门人”，使NO在光热激活下释放。Cu2+消耗细胞内谷胱甘肽，催化芬顿样反应生成羟基自由基（•OH），从而放大氧化应激。体外研究证实了有效的细胞摄取，按需NO和活性氧（ROS）的产生，以及协同细胞毒性。经组织病理学和血液生化验证，体内评估显示PTT/化疗/气体治疗/CDT联合治疗具有显著的肿瘤抑制作用，且系统毒性最小。该设计体现了一种精确的纳米医学策略，该策略使用内源性刺激和外源性激活来有效地组合治疗癌症。

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引用次数: 0

Harnessing lipid based nanocochleates for targeted therapeutic delivery: Addressing formulation bottlenecks and advancements 利用脂基纳米酸盐靶向治疗递送：解决配方瓶颈和进步。

IF 5.6 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces

Pub Date : 2025-11-19 DOI: 10.1016/j.colsurfb.2025.115287

Vishal B. Mahanur , Nikhil Bhimsing Khandale , Mayur Sanjay Tekade , Sachin Kumar Singh

Novel lipid-based drug delivery systems have gained considerable attention in recent years, with nanocochleates are emerging as a particularly promising lipid-based delivery system, recognized for their ability to enhance the stability, bioavailability, and site-directed delivery of a broad spectrum of therapeutic and nutraceutical agents. Nanocochleates are supramolecular, cylindrical lipid-based carriers formed through the interaction of negatively charged phospholipid such as phosphatidylserine with divalent cations like calcium. Their unique multilamellar structure imparts high physicochemical stability, enabling efficient encapsulation and protection of both hydrophilic and hydrophobic bioactives from environmental and physiological degradation. Compared to conventional lipid-based systems such as liposomes or solid lipid nanoparticles, nanocochleates provide sustained release, improved gastrointestinal stability, and reduced systemic toxicity, making them suitable for oral, mucosal, and systemic administration. Applications include both pharmaceutical and nutraceutical sectors, including functional foods, where nanocochleates enhance the solubility, sensory properties, and shelf-life of bioactives such as curcumin, vitamin D₃, and omega-3 fatty acids. Various formulation techniques, including the liposome-to-cochleate transition, trapping methods, and freeze-drying, have been developed to optimize particle size, entrapment efficiency, and release behavior. Despite their potential, key challenges remain in the form of large-scale production, batch consistency, regulatory validation, and long-term safety assessment. This review highlights the formulation strategies, functional attributes, and biomedical relevance of nanocochleates while addressing their current limitations and translational prospects. Their distinct advantages underscore the importance of continued interdisciplinary efforts toward realizing their full potential in drug delivery and nutrition-based interventions.

近年来，新型的脂质给药系统获得了相当多的关注，纳米酸盐作为一种特别有前途的脂质给药系统，因其提高稳定性、生物利用度和广谱治疗和营养制剂的定点递送能力而得到认可。纳米酸盐是由带负电荷的磷脂（如磷脂酰丝氨酸）与二价阳离子（如钙）相互作用形成的超分子圆柱形脂基载体。其独特的多层结构赋予了高的物理化学稳定性，能够有效地封装和保护亲水性和疏水性生物活性物质免受环境和生理降解。与传统的脂质系统（如脂质体或固体脂质纳米颗粒）相比，纳米蜗酸盐提供持续释放，改善胃肠道稳定性，降低全身毒性，使其适合口服，粘膜和全身给药。纳米酸盐的应用包括制药和营养保健部门，包括功能食品，其中纳米酸盐提高了姜黄素、维生素D₃和omega-3脂肪酸等生物活性物质的溶解度、感官特性和保质期。各种配方技术，包括脂质体到耳蜗酸盐的转变、捕获方法和冷冻干燥，已经开发出优化粒径、捕获效率和释放行为的方法。尽管它们具有潜力，但主要挑战仍然存在于大规模生产、批次一致性、监管验证和长期安全性评估方面。这篇综述强调了纳米螯合物的配方策略、功能属性和生物医学相关性，同时指出了它们目前的局限性和转化前景。它们的独特优势强调了持续跨学科努力的重要性，以实现它们在药物输送和营养干预方面的全部潜力。

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