Pub Date : 2010-12-01DOI: 10.1109/ICSMB.2010.5735369
Krishna Dalal, D. Elanchezhiyan, A. Dey, V. Bharathi Maran, A. Srivastava, M. Tripathi
This study aims at estimating the efficacy of reflexology in managing pain under 4 diseased conditions viz., mastalgia, osteoarthritis, neuropathy with type-II diabetic mellitus and lower limb pain involving intractable epilepsy. The efficiency of reflexology was determined in terms of improvement of pain score, quality of life and the other associated symptoms. The sample size of the randomized clinical trials is 220. The control group patients (N=118) received conventional medicines and study group patients (N=102) received reflexology in addition. Reflexology was administered to the patients by their caregivers at their own places. An initial period of 2½ – 3 months was devoted for (a) training the caregivers and (b) monitoring the quality control of compliances. Follow up period started after this duration. In each category of the ailments, the patients were distributed into two groups blindly through random number generation. The data records were collected prior to the therapy, intermittent and at the end of follow up period. The functional statuses of the internal organs were diagnosed using reflexology method so that all the study group patients could be treated holistically. The data were analyzed statistically and compared. It was observed that there are at least two fold improvements in the pain management, quality of life and other symptoms with statistically significant values. The results of these trials lead to conclude that reflexology in addition to or alone may offer a holistic therapy in pain management with least cost and insignificant side effects.
{"title":"Efficacy of reflexology in managing chronic pain and difficulty in lower limb movement involving intractable epilepsy","authors":"Krishna Dalal, D. Elanchezhiyan, A. Dey, V. Bharathi Maran, A. Srivastava, M. Tripathi","doi":"10.1109/ICSMB.2010.5735369","DOIUrl":"https://doi.org/10.1109/ICSMB.2010.5735369","url":null,"abstract":"This study aims at estimating the efficacy of reflexology in managing pain under 4 diseased conditions viz., mastalgia, osteoarthritis, neuropathy with type-II diabetic mellitus and lower limb pain involving intractable epilepsy. The efficiency of reflexology was determined in terms of improvement of pain score, quality of life and the other associated symptoms. The sample size of the randomized clinical trials is 220. The control group patients (N=118) received conventional medicines and study group patients (N=102) received reflexology in addition. Reflexology was administered to the patients by their caregivers at their own places. An initial period of 2½ – 3 months was devoted for (a) training the caregivers and (b) monitoring the quality control of compliances. Follow up period started after this duration. In each category of the ailments, the patients were distributed into two groups blindly through random number generation. The data records were collected prior to the therapy, intermittent and at the end of follow up period. The functional statuses of the internal organs were diagnosed using reflexology method so that all the study group patients could be treated holistically. The data were analyzed statistically and compared. It was observed that there are at least two fold improvements in the pain management, quality of life and other symptoms with statistically significant values. The results of these trials lead to conclude that reflexology in addition to or alone may offer a holistic therapy in pain management with least cost and insignificant side effects.","PeriodicalId":297136,"journal":{"name":"2010 International Conference on Systems in Medicine and Biology","volume":"44 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117274016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2010-12-01DOI: 10.1109/ICSMB.2010.5735412
S. Kumar Jana, B. Chakravarty, K. Chaudhury
It is well established that oxidative stress (OS) plays a major role in male infertility. OS occurs when the production of potentially destructive reactive oxygen species (ROS) exceeds the body's own natural antioxidant defenses, resulting in cellular damage. Antioxidant and trace element supplementation to infertile men has shown considerable improvement in sperm parameters; however, the issue of oral administration of antioxidants as therapy for male sub-fertility still remains a debate. Also, very recently, there has been an alarming report on the adverse effect of antioxidant administration by a group of researchers from the Copenhagen University (2007). One of the main reasons for an increase in the risk of death may be excessive interference with the natural defense mechanism within the body by knocking out ‘free radicals’. Also, commercially available antioxidants are expensive for a developing country like India. Hence, study of the actual efficacy of oral antioxidant consisted of lycopene, carotene, wheat germ oil, calcium ascorbate, vitamin B12, selenium and zinc needs further attention.
{"title":"Does oral antioxidant administration improve semen parameters in infertile men?","authors":"S. Kumar Jana, B. Chakravarty, K. Chaudhury","doi":"10.1109/ICSMB.2010.5735412","DOIUrl":"https://doi.org/10.1109/ICSMB.2010.5735412","url":null,"abstract":"It is well established that oxidative stress (OS) plays a major role in male infertility. OS occurs when the production of potentially destructive reactive oxygen species (ROS) exceeds the body's own natural antioxidant defenses, resulting in cellular damage. Antioxidant and trace element supplementation to infertile men has shown considerable improvement in sperm parameters; however, the issue of oral administration of antioxidants as therapy for male sub-fertility still remains a debate. Also, very recently, there has been an alarming report on the adverse effect of antioxidant administration by a group of researchers from the Copenhagen University (2007). One of the main reasons for an increase in the risk of death may be excessive interference with the natural defense mechanism within the body by knocking out ‘free radicals’. Also, commercially available antioxidants are expensive for a developing country like India. Hence, study of the actual efficacy of oral antioxidant consisted of lycopene, carotene, wheat germ oil, calcium ascorbate, vitamin B12, selenium and zinc needs further attention.","PeriodicalId":297136,"journal":{"name":"2010 International Conference on Systems in Medicine and Biology","volume":"6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116907199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2010-12-01DOI: 10.1109/ICSMB.2010.5735350
J. Satija, Gauri M. Shukla, S. Mukherji
The aim of this paper is to study the effect of dendrimeric and non-dendrimeric support in surface plasmon resonance (SPR) sensors for proteineous antigen analysis. 11-mercapto undecanoic acid (MUA) linker was chosen to fabricate the non-dendrimeric sensor surface while fourth generation poly(amidoamine) (PAMAM) dendrimer was used to design the dendrimeric sensor support on gold disks. Both the surfaces were conjugated with human immunoglobulin G (HIgG) antibodies and the binding was monitored in real time on Autolab Twingle SPR system. Antibodies were covalently attached to the MUA surface using EDC-NHS chemistry while glutaraldehyde was used as linker to conjugate antibodies on MUA-PAMAM surface. Further, both the sensor supports were used to study the antigen-antibody interaction using goat anti-human immunoglobulin (GaHIgG) antigen as sample analyte. Through SPR measurements, we found that dendrimeric sensor support exhibited two fold enhanced signal for HIgG binding than that of MUA surface only. During GaHIgG interaction, dendrimer modified sensor surface displayed enhanced signal with respect to the non-dendrimeric surface. This enhanced sensor response of dendrimeric SPR sensor support is attributed to high functional group density, globular shape and greater accessibility of immobilized probe towards analyte interaction.
{"title":"Potential of dendrimeric architecture in surface plasmon resonance biosensor","authors":"J. Satija, Gauri M. Shukla, S. Mukherji","doi":"10.1109/ICSMB.2010.5735350","DOIUrl":"https://doi.org/10.1109/ICSMB.2010.5735350","url":null,"abstract":"The aim of this paper is to study the effect of dendrimeric and non-dendrimeric support in surface plasmon resonance (SPR) sensors for proteineous antigen analysis. 11-mercapto undecanoic acid (MUA) linker was chosen to fabricate the non-dendrimeric sensor surface while fourth generation poly(amidoamine) (PAMAM) dendrimer was used to design the dendrimeric sensor support on gold disks. Both the surfaces were conjugated with human immunoglobulin G (HIgG) antibodies and the binding was monitored in real time on Autolab Twingle SPR system. Antibodies were covalently attached to the MUA surface using EDC-NHS chemistry while glutaraldehyde was used as linker to conjugate antibodies on MUA-PAMAM surface. Further, both the sensor supports were used to study the antigen-antibody interaction using goat anti-human immunoglobulin (GaHIgG) antigen as sample analyte. Through SPR measurements, we found that dendrimeric sensor support exhibited two fold enhanced signal for HIgG binding than that of MUA surface only. During GaHIgG interaction, dendrimer modified sensor surface displayed enhanced signal with respect to the non-dendrimeric surface. This enhanced sensor response of dendrimeric SPR sensor support is attributed to high functional group density, globular shape and greater accessibility of immobilized probe towards analyte interaction.","PeriodicalId":297136,"journal":{"name":"2010 International Conference on Systems in Medicine and Biology","volume":"55 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126630755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2010-12-01DOI: 10.1109/ICSMB.2010.5735390
Rohit Nayak, V. Shenoy, R. R. Galigekere
This paper describes a new algorithm for automatic assessment of the degree of TB-infection, by counting the number of Mycobacteria i.e., acid fast bacilli (AFB) in the color images of ZN-stained sputum smear. This algorithm consists of two stages. The first (“pre-processing”) stage involves exploiting color information to segment the candidate AFB in the image from the background, based on classification of pixels in the HSI color-space using Mahalanobis distance. In this context, we introduce a novel “divide and conquer” strategy to improve the robustness of color-classification. The pre-processing stage is followed by connected component labeling, size-thresholding to remove noisy objects, proximity-grouping by using a novel proximity-test algorithm, and area-based classification. Our algorithm identifies and counts the number of AFB irrespective of their shapes, can handle bacilli with beaded structure (which are important and are specific to TB) and has shown reasonable success in isolating clumps. A total of 205 images of ZN-stained sputum smears taken from more than 12 patients were considered in our experiments. Results on 169 images, based on HSI clusters built from 36 other images, are encouraging. Some of the images used in building the data-base and also in validation, include those sent by the RNTCP (Govt. of India) for training-purposes.
{"title":"A new algorithm for automatic assessment of the degree of TB-infection using images of ZN-stained sputum smear","authors":"Rohit Nayak, V. Shenoy, R. R. Galigekere","doi":"10.1109/ICSMB.2010.5735390","DOIUrl":"https://doi.org/10.1109/ICSMB.2010.5735390","url":null,"abstract":"This paper describes a new algorithm for automatic assessment of the degree of TB-infection, by counting the number of Mycobacteria i.e., acid fast bacilli (AFB) in the color images of ZN-stained sputum smear. This algorithm consists of two stages. The first (“pre-processing”) stage involves exploiting color information to segment the candidate AFB in the image from the background, based on classification of pixels in the HSI color-space using Mahalanobis distance. In this context, we introduce a novel “divide and conquer” strategy to improve the robustness of color-classification. The pre-processing stage is followed by connected component labeling, size-thresholding to remove noisy objects, proximity-grouping by using a novel proximity-test algorithm, and area-based classification. Our algorithm identifies and counts the number of AFB irrespective of their shapes, can handle bacilli with beaded structure (which are important and are specific to TB) and has shown reasonable success in isolating clumps. A total of 205 images of ZN-stained sputum smears taken from more than 12 patients were considered in our experiments. Results on 169 images, based on HSI clusters built from 36 other images, are encouraging. Some of the images used in building the data-base and also in validation, include those sent by the RNTCP (Govt. of India) for training-purposes.","PeriodicalId":297136,"journal":{"name":"2010 International Conference on Systems in Medicine and Biology","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125856419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2010-12-01DOI: 10.1109/ICSMB.2010.5735386
S. P. Chaudhury, Debjani Chakraborty, Arindam Mukhopadhyay, Debashree Guha, J. Chatterjee
The problem of arsenic pollution in the ground water of West Bengal (WB), India may be a geochemical happening, but the matter is yet unsolved. To identify correlation amongst the ground water parameters (GWP), fuzzy regression analytical technique has been utilized and it is established that Na, K, Fe, pH and hardness have distinct correlations in arsenic prone areas and non-arsenic prone areas.
{"title":"Analyzing important ground water parameters in West Bengal with a fuzzy approach in the context of arsenic pollution","authors":"S. P. Chaudhury, Debjani Chakraborty, Arindam Mukhopadhyay, Debashree Guha, J. Chatterjee","doi":"10.1109/ICSMB.2010.5735386","DOIUrl":"https://doi.org/10.1109/ICSMB.2010.5735386","url":null,"abstract":"The problem of arsenic pollution in the ground water of West Bengal (WB), India may be a geochemical happening, but the matter is yet unsolved. To identify correlation amongst the ground water parameters (GWP), fuzzy regression analytical technique has been utilized and it is established that Na, K, Fe, pH and hardness have distinct correlations in arsenic prone areas and non-arsenic prone areas.","PeriodicalId":297136,"journal":{"name":"2010 International Conference on Systems in Medicine and Biology","volume":"22 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133419922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2010-12-01DOI: 10.1109/ICSMB.2010.5735398
P. Siddalingaswamy, K. G. Prabhu
Diabetic maculopathy is the major cause of irreversible vision loss due to retinopathy and is found in 10% of the world diabetic population. Compulsory mass screening will help to identify the maculopathy at early stage and reduce the risk of severe vision loss. In this paper, we present a computer based system for automatic detection and grading of diabetic maculopathy severity level without manual intervention. The optic disc is detected automatically and its location and diameter is used to detect fovea and to mark the macular region respectively. Next, hard exudates are detected using clustering and mathematical morphological techniques. Based on the location of exudates in marked macular region the severity level of maculopathy is classified into mild, moderate and severe. The method achieves a sensitivity of 95.6% and specificity of 96.15% with 148 retinal images for detecting maculopathy stages in fundus images as comparable to that of human expert.
{"title":"Automatic grading of diabetic maculopathy severity levels","authors":"P. Siddalingaswamy, K. G. Prabhu","doi":"10.1109/ICSMB.2010.5735398","DOIUrl":"https://doi.org/10.1109/ICSMB.2010.5735398","url":null,"abstract":"Diabetic maculopathy is the major cause of irreversible vision loss due to retinopathy and is found in 10% of the world diabetic population. Compulsory mass screening will help to identify the maculopathy at early stage and reduce the risk of severe vision loss. In this paper, we present a computer based system for automatic detection and grading of diabetic maculopathy severity level without manual intervention. The optic disc is detected automatically and its location and diameter is used to detect fovea and to mark the macular region respectively. Next, hard exudates are detected using clustering and mathematical morphological techniques. Based on the location of exudates in marked macular region the severity level of maculopathy is classified into mild, moderate and severe. The method achieves a sensitivity of 95.6% and specificity of 96.15% with 148 retinal images for detecting maculopathy stages in fundus images as comparable to that of human expert.","PeriodicalId":297136,"journal":{"name":"2010 International Conference on Systems in Medicine and Biology","volume":"42 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132173325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2010-12-01DOI: 10.1109/ICSMB.2010.5735348
Sayan Dey, Goutam Saha
The Bayesian belief network is a powerful knowledge representation and reasoning tool under conditions of uncertainty to analyze gene expression patterns. Nowadays, this is an important tool to construct mathematical models based on probability to identify any particular dominant Genetic Network of any organism under observation. The present study deals with analysis of a set of micro array data collected at a regular interval of time throughout the growth phase of a fungus Burkholderia pseudomalli. In the first phase of the study, emphasis was given to recover a set of most dominant genes among the set of all possible expressed genes found in the microarray experiment. These dominant genes are then used to find out a dominant Genetic Network by applying the Bayesian Algorithm. Thus, the most dominant genetic network for the growth and development of the fungus under consideration was obtained. The genetic network represents the set of responsible genes in the growth process and their inter relationships. The Microarray data set represents the external manifestation of internal genetic activity resulting into genetic network. Here, from the set of 5289 genes in 47 consecutive time instances, were taken for analysis. Out of them, 400 most pertinent genes for the growth process were determined using a new technique namely ‘Fidelity Matrix Process’. Genetic Network for these 400 genes has been constructed and studied using Bayesian Belief Network Technique. The present reduction method was found to be more efficient in terms of computation when compared contemporary studies done many scientists. The results of the present study may be extensively applied in reducing a huge number of genetic expression rate data without any complex computation, studying unknown biological processes and systems, treating complicated diseases and even designing drugs for some incorrigible syndromes.
{"title":"Determination and study of a dominant Genetic Network responsible for the growth of a fungus using the concepts of Bayesian Algorithm","authors":"Sayan Dey, Goutam Saha","doi":"10.1109/ICSMB.2010.5735348","DOIUrl":"https://doi.org/10.1109/ICSMB.2010.5735348","url":null,"abstract":"The Bayesian belief network is a powerful knowledge representation and reasoning tool under conditions of uncertainty to analyze gene expression patterns. Nowadays, this is an important tool to construct mathematical models based on probability to identify any particular dominant Genetic Network of any organism under observation. The present study deals with analysis of a set of micro array data collected at a regular interval of time throughout the growth phase of a fungus Burkholderia pseudomalli. In the first phase of the study, emphasis was given to recover a set of most dominant genes among the set of all possible expressed genes found in the microarray experiment. These dominant genes are then used to find out a dominant Genetic Network by applying the Bayesian Algorithm. Thus, the most dominant genetic network for the growth and development of the fungus under consideration was obtained. The genetic network represents the set of responsible genes in the growth process and their inter relationships. The Microarray data set represents the external manifestation of internal genetic activity resulting into genetic network. Here, from the set of 5289 genes in 47 consecutive time instances, were taken for analysis. Out of them, 400 most pertinent genes for the growth process were determined using a new technique namely ‘Fidelity Matrix Process’. Genetic Network for these 400 genes has been constructed and studied using Bayesian Belief Network Technique. The present reduction method was found to be more efficient in terms of computation when compared contemporary studies done many scientists. The results of the present study may be extensively applied in reducing a huge number of genetic expression rate data without any complex computation, studying unknown biological processes and systems, treating complicated diseases and even designing drugs for some incorrigible syndromes.","PeriodicalId":297136,"journal":{"name":"2010 International Conference on Systems in Medicine and Biology","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134338828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2010-12-01DOI: 10.1109/ICSMB.2010.5735349
Harshit Popat, Tanuj Poshwal, Megha Singh, N. Nawraj, J. Indumathi
The interactions of laser radiation with pathological slide reveal their diverse optical characteristic depending on their compositional variation. Their point to point compositional variation is determined by reconstruction of the reflectance images which carry valuable information. However conventional microscope only deals with giving the image as an enlarged form, and is also light dependent. The system of confocal imaging is very complex and involves large number of lenses. Surface reflected laser radiations from slide are measured by a reflectance imager. Laser radiations (680 nm) are guided by an optical fiber inclined at 45 degree angle and reflected radiations are collected by photo detectors placed at an angle of 45 degree to the source which is also at 45 degree. An array of source and detectors (i.e. 12 source and 12 detectors) are placed in a probe which is stationary. The slide is placed on a platform which moves in steps of 1mm.The platform moves and data representing two-dimensional variation are collected, after amplification, these are digitized, and post processing of the images is done. An advanced algorithm of image processing is used and color coding is done. The probe design is highly refined and first of its kind. The accuracy is high since the source is stationary. The reflected image can be inferred by the processing technique which gives useful parameters. This system is capable of producing output equivalent to C.T-scan or M.R.I in one-fifth of their cost.
{"title":"Laser reflectance microscopy 2-D tissue imaging system","authors":"Harshit Popat, Tanuj Poshwal, Megha Singh, N. Nawraj, J. Indumathi","doi":"10.1109/ICSMB.2010.5735349","DOIUrl":"https://doi.org/10.1109/ICSMB.2010.5735349","url":null,"abstract":"The interactions of laser radiation with pathological slide reveal their diverse optical characteristic depending on their compositional variation. Their point to point compositional variation is determined by reconstruction of the reflectance images which carry valuable information. However conventional microscope only deals with giving the image as an enlarged form, and is also light dependent. The system of confocal imaging is very complex and involves large number of lenses. Surface reflected laser radiations from slide are measured by a reflectance imager. Laser radiations (680 nm) are guided by an optical fiber inclined at 45 degree angle and reflected radiations are collected by photo detectors placed at an angle of 45 degree to the source which is also at 45 degree. An array of source and detectors (i.e. 12 source and 12 detectors) are placed in a probe which is stationary. The slide is placed on a platform which moves in steps of 1mm.The platform moves and data representing two-dimensional variation are collected, after amplification, these are digitized, and post processing of the images is done. An advanced algorithm of image processing is used and color coding is done. The probe design is highly refined and first of its kind. The accuracy is high since the source is stationary. The reflected image can be inferred by the processing technique which gives useful parameters. This system is capable of producing output equivalent to C.T-scan or M.R.I in one-fifth of their cost.","PeriodicalId":297136,"journal":{"name":"2010 International Conference on Systems in Medicine and Biology","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134080827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2010-12-01DOI: 10.1109/ICSMB.2010.5735371
E. Subramani, K. Chaudhury
Oxidative stress (OS) indicates an imbalance between the generation of reactive oxygen species (ROS) and total antioxidant capacity (TAC). ROS and TAC levels, lipid peroxidation (LPO), acrosome reaction status and OS induced DNA damage were assessed in normozoospermic infertile men (Group I) and compared with proven fertile men (Group II; controls). Forty men with normal semen parameters were grouped into normozoospermic infertile men (n=30) and proven fertile men (n=10). ROS and TAC levels were measured by chemiluminescence assay. LPO, acrosome reaction and DNA fragmentation were assessed and compared with ROS generation. Differences between these groups was calculated using two tailed Students t-test and statistical significance assessed at P value<0.05. Significant increase in ROS and decrease in TAC levels were observed in Group I patients as compared to Group II. These results correlated well with LPO results. Further, TAC was found to be reduced in cases with high ROS levels. DNA fragmentation was found to be positively correlated with ROS. A sharp decline in the acrosomal integrity was observed in Group II. Decreased number of acrosome reacted spermatozoa and increased LPO play a significant role in the etiology of normozoospermic infertile men. In conclusion, ROS and TAC are expected to play an important role in male infertility and may have an impact on the failure of conception in women having male partners with normal semen parameters.
{"title":"Detrimental effects of oxidative stress on sperm membrane and DNA integrity in normozoospermic infertile men","authors":"E. Subramani, K. Chaudhury","doi":"10.1109/ICSMB.2010.5735371","DOIUrl":"https://doi.org/10.1109/ICSMB.2010.5735371","url":null,"abstract":"Oxidative stress (OS) indicates an imbalance between the generation of reactive oxygen species (ROS) and total antioxidant capacity (TAC). ROS and TAC levels, lipid peroxidation (LPO), acrosome reaction status and OS induced DNA damage were assessed in normozoospermic infertile men (Group I) and compared with proven fertile men (Group II; controls). Forty men with normal semen parameters were grouped into normozoospermic infertile men (n=30) and proven fertile men (n=10). ROS and TAC levels were measured by chemiluminescence assay. LPO, acrosome reaction and DNA fragmentation were assessed and compared with ROS generation. Differences between these groups was calculated using two tailed Students t-test and statistical significance assessed at P value<0.05. Significant increase in ROS and decrease in TAC levels were observed in Group I patients as compared to Group II. These results correlated well with LPO results. Further, TAC was found to be reduced in cases with high ROS levels. DNA fragmentation was found to be positively correlated with ROS. A sharp decline in the acrosomal integrity was observed in Group II. Decreased number of acrosome reacted spermatozoa and increased LPO play a significant role in the etiology of normozoospermic infertile men. In conclusion, ROS and TAC are expected to play an important role in male infertility and may have an impact on the failure of conception in women having male partners with normal semen parameters.","PeriodicalId":297136,"journal":{"name":"2010 International Conference on Systems in Medicine and Biology","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131364528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2010-12-01DOI: 10.1109/ICSMB.2010.5735360
M. S. Mallikarjunaswamy, M. Holi
In the human body knees are most complex and delicate joints. Knee joints are frequently injured and damaged due to articulations. The knees are among the joints most commonly affected by osteoarthritis (OA). Articular Cartilage is a hard but slippery coating on the end of each bone which forms the joint. Cartilage breaks down and wears away in OA. In this work, image processing techniques are applied on magnetic resonance images (MRI) of knee. Histogram equalization, thresholding, region of interest (ROI) processing and Canny edge detection techniques are adopted. This method segments cartilage from femur, tibia and menisci. It is semi-automatic method for visualization of knee cartilage. Quantification of cartilage thickness is carried out for normal and OA cases. The results are useful in the study of progression of OA and for therapeutic decisions.
{"title":"Knee joint cartilage visualization and quantification in normal and osteoarthritis","authors":"M. S. Mallikarjunaswamy, M. Holi","doi":"10.1109/ICSMB.2010.5735360","DOIUrl":"https://doi.org/10.1109/ICSMB.2010.5735360","url":null,"abstract":"In the human body knees are most complex and delicate joints. Knee joints are frequently injured and damaged due to articulations. The knees are among the joints most commonly affected by osteoarthritis (OA). Articular Cartilage is a hard but slippery coating on the end of each bone which forms the joint. Cartilage breaks down and wears away in OA. In this work, image processing techniques are applied on magnetic resonance images (MRI) of knee. Histogram equalization, thresholding, region of interest (ROI) processing and Canny edge detection techniques are adopted. This method segments cartilage from femur, tibia and menisci. It is semi-automatic method for visualization of knee cartilage. Quantification of cartilage thickness is carried out for normal and OA cases. The results are useful in the study of progression of OA and for therapeutic decisions.","PeriodicalId":297136,"journal":{"name":"2010 International Conference on Systems in Medicine and Biology","volume":"74 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114842104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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