Advanced Nanobiomed Research最新文献

Chemo-Magneto-Photothermal Therapy

This work presents maghemite nanoparticles functionalized with doxorubicin as a trimodal nanoplatform that combines magnetic hyperthermia, photothermal therapy and localized chemotherapy under clinically safe field and laser conditions. By triggering pH-sensitive drug release and synergistic heating inside cancer cells, these nanoparticles achieve potent tumor cell killing while minimizing systemic toxicity. More details can be found in the Research Article by Ana Espinosa and co-workers (DOI: 10.1002/anbr.202500098).

Adv. NanoBiomed Res., 2025, 5(11), 2500118

https://doi.org/10.1002/anbr.202500118

Figures 4 and 5 have been updated.

The third paragraph under the section “2.3. Release of sequestered dye product from Pd-laden hydrogels” has been updated:

The proportion of sequestered dye released from pHEMA–GG discs significantly increased from 12.6% to 17.9% to 21.1% (averaged over both thicknesses and Pd nanoshapes) when exposure time to proresorufin increased from 0.5 to 1 to 2 hours (p < 0.0001). For the pHEMA–co–AM copolymers, the increase from 16.3% after 0.5 hours to 18.3% after 1 hours was smaller but still significant (p = 0.0001), whereas the increase to 18.6% at 2 hours was not (p = 0.8, Figure 5).

The section “5.10. Statistics” has been updated.

All values are reported as average ± standard deviation unless indicated otherwise. Reported p-values for head-to-head comparisons are obtained from two-tailed homoscedastic Student's T-test (MS Excel). ANOVA with Tukey's post-hoc test was performed between time-points, for pHEMA-GG and pHEMA-co-AM polymers separately (MS Excel). Groups that share a letter (and case) are not statistically significantly different. Decreasing trends were tested for statistical significance using a Mann-Kendall test (XLSTAT).

Mechanoadaptive Responses

In this cover image, the newly found mechanoadaptive response of neutrophils adhesion and the membrane tether coalescence MI-SIM super-resolution images under abrupt flow acceleration are emphasized and represented. More details can be found in the Research Article by Lining Arnold Ju and co-workers (DOI: 10.1002/anbr.202500113).

Adv. NanoBiomed Res. 2025, 5(9), 2500066

https://doi.org/10.1002/anbr.202500066

The supplementary material for this article has been updated.

Biomaterial-associated infections, particularly those involving methicillin-resistant Staphylococcus aureus, present a significant challenge in tissue engineering, often leading to implant failure. This review examines the strategic development of vancomycin (VAN)-loaded nanoplatforms as an advanced modality to augment antibacterial efficacy within tissue engineering scaffolds. Conventional antibiotic delivery methods are frequently limited by suboptimal drug release kinetics, systemic toxicity, and inadequate penetration of bacterial biofilms. Nanotechnology-based approaches, including polymeric nanoparticles, liposomes, and nanofibers, offer a sophisticated solution by enabling targeted, localized, and sustained release of VAN directly at the implantation site. These systems significantly enhance VAN's bioavailability, reduce requisite dosages, thereby mitigating cytotoxic effects on progenitor cells, and effectively disrupt biofilm matrices. The integration of these nanocarriers into biomaterial matrices, such as hydrogels and electrospun scaffolds, creates a multifunctional environment that concurrently supports tissue regeneration and provides robust prophylactic and therapeutic antimicrobial action.

Craniofacial Tissue Engineering

This research illustrates an innovative strategy for immediate tissue repair using a 3D-printed scaffold infused with primary stem cells-laden hydrogels. Without prior culture, the construct promotes rapid tissue integration and vascularization upon implantation in vivo. This approach represents a promising advancement for emergency craniofacial tissue regeneration, enabling volumetric tissue healing through direct and effective stem cell transplantation. More details can be found in the Research Article by Sangjin Lee and co-workers (DOI: 10.1002/anbr.202500006).

Neutrophils navigating the vasculature encounter regions of abrupt flow acceleration that challenge their adhesive capacity. Here, a previously uncharacterized mechanoadaptive response that enables neutrophils to maintain adhesion under these challenging conditions is revealed. Using microfluidic systems to precisely control flow dynamics, it is demonstrated that neutrophils respond differently to steady versus accelerating flow (delta shear) conditions. While steady-increasing flow induces formation of multiple discrete tethers, abrupt acceleration triggers their coalescence into thicker, mechanically robust structures that significantly enhance adhesion stability. Through Machine Intelligent Structured Illumination Microscopy with exceptional spatiotemporal resolution, the nanoscale dynamics of this coalescence process is characterized, revealing that despite extensive membrane remodeling, the original anchor points of adhesion molecules remain spatially fixed. Dual-color spinning total internal reflection fluorescence imaging shows targeted accumulation of F-actin at the cell tongue, providing critical mechanical support. Differential effects of actin-disrupting agents confirm that tether coalescence depends on intact cytoskeletal structures rather than active polymerization. This membrane adaptation represents a sophisticated strategy enabling neutrophils to withstand high detachment forces in disturbed flow environments characteristic of vascular bifurcations, stenoses, and device-associated thromboinflammation. These findings advance understanding of neutrophil mechanobiology and may inform therapeutic strategies targeting pathological neutrophil adhesion without compromising essential immune functions.

The rapid advancement in nanotechnology over the past three decades has accelerated the development of novel and more potent cancer treatments. In particular, this has led to the development of an increasing number of novel carrier systems for drug delivery, among which Pickering emulsions are a strong contender. This article reviews the development, characterization, and therapeutic efficacy of Pickering emulsion nanocarriers designed specifically for cancer treatment. This approach offers significant benefits for overcoming many of the challenges experienced in conventional drug delivery systems for cancer therapy. The mechanisms of drug release, targeting strategies, and the stability of Pickering emulsions under physiological conditions are examined, along with an evaluation of their therapeutic potential and biocompatibility of these nanocarriers in various cancer models with in vitro and in vivo studies. The ability of Pickering emulsions to improve therapeutic efficacy through encapsulation and protection of hydrophobic drugs is also highlighted, resulting in targeted drug release at the tumor site and minimal side effects. Future development of the nanocarrier systems must address the challenges of achieving large-scale production, regulatory approval, and translational application. If successfully addressed, it will pave the way for making personalized delivery of therapeutics for cancer treatment a reality.

Pathological scarring imposes a substantial global healthcare burden, affecting over 100 million individuals annually with costs exceeding $20 billion. Current therapies yield suboptimal outcomes due to limited efficacy and recurrence. Hydrogel-based wound dressings have emerged as transformative platforms due to their tunable physicochemical properties, bioactivity, and ability to modulate the wound microenvironment. This review uniquely integrates scar biology with hydrogel-based therapeutic strategies. A phase-specific framework that correlates hydrogel functions is provided with key scar-influencing events, including inflammation regulation, fibroblast reprograming, extracellular matrix remodeling, and skin appendage regeneration. Moreover, cutting-edge innovations are highlighted such as stimuli-responsive hydrogels (pH/temperature/light), nanocomposite systems, and 3D-printed scaffolds that enable spatiotemporal control of drug release and dynamic microenvironment modulation. Furthermore, unresolved clinical translation barriers are critically addressed, including scalability, standardization, biocompatibility, and immune response variability, proposing interdisciplinary solutions. By synthesizing recent advances and persistent limitations, this work provides a translational roadmap for developing next-generation hydrogels to bridge the gap between benchtop innovation and clinical scar-free tissue regeneration.

A magnetically actuated DNA release platform employing sustainable walnut shell–derived electrodes enables precise ON/OFF switching of DNA release through magnetic–enzymatic filter beads, offering a controllable and reusable system for bioelectronic and sensing applications. More details can be found in the Research Article by Paolo Bollella, Luisa Torsi, and co-workers (DOI: 10.1002/anbr.202500131).