Pub Date : 2020-12-23DOI: 10.22435/hsji.v11i2.3072
S. Maemun, N. Mariana, S. Wijaya, Dina Oktavia, V. Lisdawati, Rita Rogayah
Latar belakang : Hipoalbuminemia pada pasien rawat inap berkaitan dengan prognosis buruk pasien. Penelitian ini, mengidentifikasi bahwa hipoalbuminemia berat pada awal pasien masuk rawat inap sebagai prediktor andalan penanda laboratorium dalam mortalitas. Metode : Sebuah studi cross sectional pada pasien dewasa dengan hipoalbuminemia (kadar albumin < 3,5 g / dL) pada pasien rawat inap (usia > 18 tahun) pada periode Januari 2013 - Maret 2018. Kami mengevaluasi penanda prediktor kematian. Multivariat dengan regresi logistik diterapkan dalam penelitian ini. Hasil : Dari 747 hipoalbuminemia pada pasien rawat inap dengan rata-rata kadar albumin pada awal adalah 2,0 ± 0,6 g / dL. Sebagian besar pasien (83,4%) memiliki kadar albumin ≤ 2,5 g / dL (hipoalbuminemia berat), 16,6 persen memiliki > 2,5 g / dL (hipoalbuminemia ringan-sedang). Kondisi yang mendasari pasien adalah infeksi HIV / AIDS (26,9%) dan sepsis (26,6%). Proporsi multiple komorbiditas pada kelompok hipoalbuminemia berat adalah 55,1 persen Pada kelompok hipoalbuminemia berat terutama untuk kadar albumin 2,01 - 2,5 g / dL, angka mortalitas adalah 28,3 persen. Berdasarkan model regresi logistik akhir, faktor risiko kematian meliputi kadar albumin pada awal dan lama rawat pasien. Mortalitas lebih tinggi pada pasien dengan hipoalbuminemia berat (rasio odds yang disesuaikan 2,91, 95% CI 1,88-4,50) dibandingkan pasien dengan hipoalbuminemia ringan-sedang. Kesimpulan: Hipoalbuminemia berat pada awal pasien rawat inap sebagai prediktor penanda kematian di rumah sakit. Kata kunci: hipoalbuminemia, pasien rawat inap, mortalitas Abstract Background: Hypoalbuminemia in hospitalized patients has been associated with poor prognosis. In this study, we attempted to identify that severe hypoalbuminemia at baseline in hospitalized patients is a reliable predictor of laboratory marker for mortality. Methods: A cross sectional study on adults of hypoalbuminemia (albumin level < 3.5 g/dL) in hospitalized patients (aged > 18 years old) in period January 2013 - March 2018. We evaluated the predictor marker of mortality. Multivariate with the logistic regression was applied in this study. Results: Of the 747 hypoalbuminemia in hospitalized patients with the mean albumin level at baseline was 2.0 ± 0.6 g/dL. Most patients (83.4 %) had less than or equal to 2.5 g/dL albumin level (severe hypoalbuminemia), 16.6 percent had over 2.5 g/dL (mild-moderate hypoalbuminemia). The underlying condition of patients was HIV/AIDS infection (26.9%) and sepsis (26.6 %). The proportion of multiple comorbidities in the severe hypoalbuminemia group was 55.1percent. In the severe hypoalbuminemia group especially for 2.01 – 2.5 g/dL albumin level, the mortality rate was 28.3 percent. Based on the final logistic regression model, known risk factors of mortality include albumin level at baseline and length of stay. Mortality was higher among patients with severe hypoalbuminemia (adjusted odds ratio 2.91, 95 % CI 1.88-4.50)
{"title":"Is hypoalbuminemia a predictor marker of mortality?","authors":"S. Maemun, N. Mariana, S. Wijaya, Dina Oktavia, V. Lisdawati, Rita Rogayah","doi":"10.22435/hsji.v11i2.3072","DOIUrl":"https://doi.org/10.22435/hsji.v11i2.3072","url":null,"abstract":"Latar belakang : Hipoalbuminemia pada pasien rawat inap berkaitan dengan prognosis buruk pasien. Penelitian ini, mengidentifikasi bahwa hipoalbuminemia berat pada awal pasien masuk rawat inap sebagai prediktor andalan penanda laboratorium dalam mortalitas. \u0000Metode : Sebuah studi cross sectional pada pasien dewasa dengan hipoalbuminemia (kadar albumin < 3,5 g / dL) pada pasien rawat inap (usia > 18 tahun) pada periode Januari 2013 - Maret 2018. Kami mengevaluasi penanda prediktor kematian. Multivariat dengan regresi logistik diterapkan dalam penelitian ini. \u0000Hasil : Dari 747 hipoalbuminemia pada pasien rawat inap dengan rata-rata kadar albumin pada awal adalah 2,0 ± 0,6 g / dL. Sebagian besar pasien (83,4%) memiliki kadar albumin ≤ 2,5 g / dL (hipoalbuminemia berat), 16,6 persen memiliki > 2,5 g / dL (hipoalbuminemia ringan-sedang). Kondisi yang mendasari pasien adalah infeksi HIV / AIDS (26,9%) dan sepsis (26,6%). Proporsi multiple komorbiditas pada kelompok hipoalbuminemia berat adalah 55,1 persen Pada kelompok hipoalbuminemia berat terutama untuk kadar albumin 2,01 - 2,5 g / dL, angka mortalitas adalah 28,3 persen. Berdasarkan model regresi logistik akhir, faktor risiko kematian meliputi kadar albumin pada awal dan lama rawat pasien. Mortalitas lebih tinggi pada pasien dengan hipoalbuminemia berat (rasio odds yang disesuaikan 2,91, 95% CI 1,88-4,50) dibandingkan pasien dengan hipoalbuminemia ringan-sedang. \u0000Kesimpulan: Hipoalbuminemia berat pada awal pasien rawat inap sebagai prediktor penanda kematian di rumah sakit. \u0000Kata kunci: hipoalbuminemia, pasien rawat inap, mortalitas \u0000 \u0000Abstract \u0000Background: Hypoalbuminemia in hospitalized patients has been associated with poor prognosis. In this study, we attempted to identify that severe hypoalbuminemia at baseline in hospitalized patients is a reliable predictor of laboratory marker for mortality. \u0000Methods: A cross sectional study on adults of hypoalbuminemia (albumin level < 3.5 g/dL) in hospitalized patients (aged > 18 years old) in period January 2013 - March 2018. We evaluated the predictor marker of mortality. Multivariate with the logistic regression was applied in this study. \u0000Results: Of the 747 hypoalbuminemia in hospitalized patients with the mean albumin level at baseline was 2.0 ± 0.6 g/dL. Most patients (83.4 %) had less than or equal to 2.5 g/dL albumin level (severe hypoalbuminemia), 16.6 percent had over 2.5 g/dL (mild-moderate hypoalbuminemia). The underlying condition of patients was HIV/AIDS infection (26.9%) and sepsis (26.6 %). The proportion of multiple comorbidities in the severe hypoalbuminemia group was 55.1percent. In the severe hypoalbuminemia group especially for 2.01 – 2.5 g/dL albumin level, the mortality rate was 28.3 percent. Based on the final logistic regression model, known risk factors of mortality include albumin level at baseline and length of stay. Mortality was higher among patients with severe hypoalbuminemia (adjusted odds ratio 2.91, 95 % CI 1.88-4.50) ","PeriodicalId":30666,"journal":{"name":"Health Science Journal of Indonesia","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42431068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-23DOI: 10.22435/hsji.v11i2.3080
K. B. Ginting, M. R. Yaznil, M. O. Prabudi, Lili Rahmawati
Latar belakang: Kanker ovarium memiliki angka mortalitas yang cukup tinggi dikarenakan gejalanya yang tidak spesifik, sering ditemukan pada stadium lanjut, dan belum adanya metode deteksi dini yang sudah terbukti. Untuk menilai keberhasilan terapi penyintas kanker ovarium, tidak hanya dinilai dari aspek klinis tetapi juga dinilai dari kualitas hidup penyintas kanker ovarium yang penilaiannya berdasarkan skala fungsional dan skala gejala dalam kuesioner EORTC QLQ C30 dan EORTC QLQ OV28. Metode: Penelitian ini menggunakan desain penelitian cross sectional, menggunakan data primer dari hasil wawancara dengan kuesioner EORTC QLQ C30 dan EORTC QLQ OV28 serta data sekunder yang berasal dari rekam medik di RSUP Haji Adam Malik Medan tahun 2017 - 2018. Sampel penelitian dipilih dengan metode total sampling dari seluruh data rekam medik yang memenuhi kriteria penelitian. Hasil: Hasil penelitian ini didapatkan kualitas hidup global penyintas kanker ovarium 89.36% adalah baik, dan 10.64% adalah sedang serta tidak ada yang memiliki kualitas hidup buruk. Namun, didapatkan adanya gangguan pada skala fungsional berupa: fungsi emosional, fungsi kognitif, fungsi seksual, dan sikap terhadap penyakit, serta adanya permasalahan pada skala gejala berupa: kelelahan, nyeri, neuropati perifer, dan gejala menopause. Didapatkan juga tidak ada hubungan karakteristik usia, jenis histopatologis, stadium, lama terapi dengan kualitas hidup penyintas kanker ovarium, namun terdapat hubungan antara jenis terapi dengan kualitas hidup penyintas kanker ovarium. Kesimpulan: Kualitas hidup penyintas kanker ovarium secara global adalah baik. Kata Kunci: Kualitas Hidup, Penyintas Kanker Ovarium, EORTC QLQ C-30, EORTC QLQ OV-28 Abstract Background: Ovarian cancer has a high mortality rate due to nonspecific symptoms, often found at an advanced stage, and also the absence of proven early detection methods. To assess the success of ovarian cancer survivors therapy, it is not only assessed from the clinical aspect but also from the quality of life of ovarian cancer survivors which is based on the functional and symptom scale in the EORTC QLQ C30 and EORTC QLQ OV28 questionnaires. Methods: This study used a cross-sectional study design, using primary data from interviews with the survivors based on the questionnaire EORTC QLQ C30 and EORTC QLQ OV28 as well as secondary data derived from medical records at Haji Adam Malik General Hospital Medan in 2017 - 2018. The research sample was used with a total sampling method from all medical record data that fulfill the research criteria. Result: The quality of life of ovarian cancer survivors is generally good (89.36%), meanwhile the rest is moderate (10.64%) without the poor quality of life. However, there are disorders on the functional scale in the form of emotional function, cognitive function, sexual function, and attitude toward disease. Likewise on the scale of symptoms, there are problems including: fatigue, pain, peripher
{"title":"Quality of life among ovarian cancer survivors in Haji Adam Malik General Hospital Medan, Indonesia","authors":"K. B. Ginting, M. R. Yaznil, M. O. Prabudi, Lili Rahmawati","doi":"10.22435/hsji.v11i2.3080","DOIUrl":"https://doi.org/10.22435/hsji.v11i2.3080","url":null,"abstract":"Latar belakang: Kanker ovarium memiliki angka mortalitas yang cukup tinggi dikarenakan gejalanya yang tidak spesifik, sering ditemukan pada stadium lanjut, dan belum adanya metode deteksi dini yang sudah terbukti. Untuk menilai keberhasilan terapi penyintas kanker ovarium, tidak hanya dinilai dari aspek klinis tetapi juga dinilai dari kualitas hidup penyintas kanker ovarium yang penilaiannya berdasarkan skala fungsional dan skala gejala dalam kuesioner EORTC QLQ C30 dan EORTC QLQ OV28. \u0000Metode: Penelitian ini menggunakan desain penelitian cross sectional, menggunakan data primer dari hasil wawancara dengan kuesioner EORTC QLQ C30 dan EORTC QLQ OV28 serta data sekunder yang berasal dari rekam medik di RSUP Haji Adam Malik Medan tahun 2017 - 2018. Sampel penelitian dipilih dengan metode total sampling dari seluruh data rekam medik yang memenuhi kriteria penelitian. \u0000Hasil: Hasil penelitian ini didapatkan kualitas hidup global penyintas kanker ovarium 89.36% adalah baik, dan 10.64% adalah sedang serta tidak ada yang memiliki kualitas hidup buruk. Namun, didapatkan adanya gangguan pada skala fungsional berupa: fungsi emosional, fungsi kognitif, fungsi seksual, dan sikap terhadap penyakit, serta adanya permasalahan pada skala gejala berupa: kelelahan, nyeri, neuropati perifer, dan gejala menopause. Didapatkan juga tidak ada hubungan karakteristik usia, jenis histopatologis, stadium, lama terapi dengan kualitas hidup penyintas kanker ovarium, namun terdapat hubungan antara jenis terapi dengan kualitas hidup penyintas kanker ovarium. \u0000Kesimpulan: Kualitas hidup penyintas kanker ovarium secara global adalah baik. \u0000Kata Kunci: Kualitas Hidup, Penyintas Kanker Ovarium, EORTC QLQ C-30, EORTC QLQ OV-28 \u0000 \u0000Abstract \u0000Background: Ovarian cancer has a high mortality rate due to nonspecific symptoms, often found at an advanced stage, and also the absence of proven early detection methods. To assess the success of ovarian cancer survivors therapy, it is not only assessed from the clinical aspect but also from the quality of life of ovarian cancer survivors which is based on the functional and symptom scale in the EORTC QLQ C30 and EORTC QLQ OV28 questionnaires. \u0000Methods: This study used a cross-sectional study design, using primary data from interviews with the survivors based on the questionnaire EORTC QLQ C30 and EORTC QLQ OV28 as well as secondary data derived from medical records at Haji Adam Malik General Hospital Medan in 2017 - 2018. The research sample was used with a total sampling method from all medical record data that fulfill the research criteria. \u0000Result: The quality of life of ovarian cancer survivors is generally good (89.36%), meanwhile the rest is moderate (10.64%) without the poor quality of life. However, there are disorders on the functional scale in the form of emotional function, cognitive function, sexual function, and attitude toward disease. Likewise on the scale of symptoms, there are problems including: fatigue, pain, peripher","PeriodicalId":30666,"journal":{"name":"Health Science Journal of Indonesia","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43672250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-23DOI: 10.22435/hsji.v11i2.2885
I. Sastrawan, Jordaniel Setiabudi, Ni Putu Gita Raditya Sanjiwani, N. Indriyani, D. A. Laksemi
Latar belakang: Infeksi kronis dari soil transmitted helminth (STH) dapat menyebabkan gangguan gizi, pertumbuhan dan kognitif pada anak. Untuk mengurangi dampak infeksi STH, diperlukan identifikasi faktor risiko. Penelitian ini bertujuan untuk mengidentifikasi faktor risiko yang berhubungan dengan infeksi STH pada siswa sekolah dasar di Desa Seraya Timur, Karangasem, Bali. Metode: Penelitian ini menggunakan desain potong lintang dengan mengambil total sampel. Penelitian dilaksanakan pada bulan Januari 2020. Data primer mengenai faktor-faktor risiko infeksi STH dikumpulkan dengan menggunakan kuesioner. Diagnosis infeksi STH dilakukan dengan pemeriksaan tinja dengan metode Kato-Katz. Analisis data menggunakan uji chi-square untuk menentukan faktor risiko yang berhubungan dengan infeksi STH. Hasil: Sebanyak 83 siswa yang berusia 6-12 tahun berpartisipasi dalam penelitian ini. Terdapat 9 siswa (10.84%) yang terinfeksi STH dengan intensitas infeksi ringan. Sebanyak 55.56% terinfeksi Trichuris trichiura, 33.33% terinfeksi Ascaris lumbricoides dan 11.11% terinfeksi cacing tambang. Terdapat beberapa faktor risiko yang memiliki hubungan bermakna dengan infeksi STH diantaranya adalah sering bermain tanah (OR=6.86; 95%CI 1.326-35.494), bermain tanpa alas kaki (OR=10.5; 95%CI 1.249-88.278) tidak mencuci tangan setelah bermain tanah (OR=9.450; 95%CI 1.809-49.358) dan tidak memotong kuku secara rutin (OR=6.462; 95%CI 1.250-33.388). Pemberian obat cacing setiap enam bulan mampu memberikan efek proteksi terhadap infeksi STH (OR=0.085; 95%CI 0.016-0.449). Kesimpulan: Kebersihan diri menjadi faktor risiko yang berhubungan dengan infeksi STH. Direkomendasikan untuk meningkatkan promosi kesehatan terkait kebersihan diri disamping pemberian obat cacing setiap enam bulan. Kata kunci: Faktor risiko, infeksi STH, anak sekolah dasar Abstract Background: Chronic soil transmitted helminth (STH) infection might cause nutritional, growth and cognitive impairment in children. Identifying the risk factors of STH infection is crucially needed to minimize the infection effects. This study aimed to identify risk factors associated with STH infections among primary school students in Seraya Timur Village, Karangasem, Bali. Methods: This study used a cross-sectional design with a total sampling method. The study was conducted in January 2020. Risk factors data were collected using a questionnaire. The diagnosis of STH infection was done by stool examination with the Kato-Katz method. The chi-square test was used to determine the risk factors associated with STH infection. Results: 83 students with ages ranging from 6-12 years participated in this study. There were 9 students (10.84%) whose infected with mild infection of STH. 55.56% of students were infected by Trichuris trichiura, 33.33% were Ascaris lumbricoides infections and 11.11% were hookworm infections. There were several risk factors that significantly associated with STH infection including ground’s playing
{"title":"Risk factors of soil transmitted helminth infection among primary school students","authors":"I. Sastrawan, Jordaniel Setiabudi, Ni Putu Gita Raditya Sanjiwani, N. Indriyani, D. A. Laksemi","doi":"10.22435/hsji.v11i2.2885","DOIUrl":"https://doi.org/10.22435/hsji.v11i2.2885","url":null,"abstract":"Latar belakang: Infeksi kronis dari soil transmitted helminth (STH) dapat menyebabkan gangguan gizi, pertumbuhan dan kognitif pada anak. Untuk mengurangi dampak infeksi STH, diperlukan identifikasi faktor risiko. Penelitian ini bertujuan untuk mengidentifikasi faktor risiko yang berhubungan dengan infeksi STH pada siswa sekolah dasar di Desa Seraya Timur, Karangasem, Bali. \u0000Metode: Penelitian ini menggunakan desain potong lintang dengan mengambil total sampel. Penelitian dilaksanakan pada bulan Januari 2020. Data primer mengenai faktor-faktor risiko infeksi STH dikumpulkan dengan menggunakan kuesioner. Diagnosis infeksi STH dilakukan dengan pemeriksaan tinja dengan metode Kato-Katz. Analisis data menggunakan uji chi-square untuk menentukan faktor risiko yang berhubungan dengan infeksi STH. \u0000Hasil: Sebanyak 83 siswa yang berusia 6-12 tahun berpartisipasi dalam penelitian ini. Terdapat 9 siswa (10.84%) yang terinfeksi STH dengan intensitas infeksi ringan. Sebanyak 55.56% terinfeksi Trichuris trichiura, 33.33% terinfeksi Ascaris lumbricoides dan 11.11% terinfeksi cacing tambang. Terdapat beberapa faktor risiko yang memiliki hubungan bermakna dengan infeksi STH diantaranya adalah sering bermain tanah (OR=6.86; 95%CI 1.326-35.494), bermain tanpa alas kaki (OR=10.5; 95%CI 1.249-88.278) tidak mencuci tangan setelah bermain tanah (OR=9.450; 95%CI 1.809-49.358) dan tidak memotong kuku secara rutin (OR=6.462; 95%CI 1.250-33.388). Pemberian obat cacing setiap enam bulan mampu memberikan efek proteksi terhadap infeksi STH (OR=0.085; 95%CI 0.016-0.449). \u0000Kesimpulan: Kebersihan diri menjadi faktor risiko yang berhubungan dengan infeksi STH. Direkomendasikan untuk meningkatkan promosi kesehatan terkait kebersihan diri disamping pemberian obat cacing setiap enam bulan. \u0000Kata kunci: Faktor risiko, infeksi STH, anak sekolah dasar \u0000 \u0000Abstract \u0000 \u0000Background: Chronic soil transmitted helminth (STH) infection might cause nutritional, growth and cognitive impairment in children. Identifying the risk factors of STH infection is crucially needed to minimize the infection effects. This study aimed to identify risk factors associated with STH infections among primary school students in Seraya Timur Village, Karangasem, Bali. \u0000Methods: This study used a cross-sectional design with a total sampling method. The study was conducted in January 2020. Risk factors data were collected using a questionnaire. The diagnosis of STH infection was done by stool examination with the Kato-Katz method. The chi-square test was used to determine the risk factors associated with STH infection. \u0000Results: 83 students with ages ranging from 6-12 years participated in this study. There were 9 students (10.84%) whose infected with mild infection of STH. 55.56% of students were infected by Trichuris trichiura, 33.33% were Ascaris lumbricoides infections and 11.11% were hookworm infections. There were several risk factors that significantly associated with STH infection including ground’s playing","PeriodicalId":30666,"journal":{"name":"Health Science Journal of Indonesia","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48132622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-18DOI: 10.22435/hsji.v11i2.3744
Y. Maladan, H. Krismawati, H. Hutapea, A. Oktavian
Latar belakang: Lepra merupakan penyakit yang disebabkan oleh Mycobacterium leprae. Resistensi obat merupakan salah satu tantangan dalam pemberantasan kusta khususnya di Papua. Adanya mutasi pada gen folP1 penyandi dihydropteroate synthase (DHPS) merupakan dasar untuk deteksi molekuler resistensi dapson pada penyakit lepra. Tujuan penelitian ini adalah mendeteksi mutasi pada gen folP1 Mycobacterium leprae dari Papua, Indonesia dan menganalisis pengaruh mutasi tersebut terhadap dapson dengan metode in silico. Metode: Identifikasi mutasi pada gen folp1 M. leprae dilakukan melalui proses Basic Local Alignment Search Tool (BLAST) di gene bank. Analisis efek mutasi dengan menggunakan server Have (y) Our Protein Explained (HOPE). Prediksi binding pocket menggunakan Computed Atlas of Surface Topography of proteins (CASTp). Homologi modeling struktur 3D DHPS menggunakan server Iterative Threading ASSEmbly Refinement (I TASSER). Analisis docking dengan menggunakan AutoDock Vina yang terintegrasi dengan aplikasi Python Prescription (PyRx). Hasil: Hasil sekuensing menunjukkan adanya variasi dalam gen folP1 M. leprae yaitu perubahan dari Timin (T) menjadi Sitosin (C) pada nukleotida 143. Residu yang bermutasi (V48A) terletak pada domain yang penting untuk aktivitas protein dan kontak dengan residu di domain lain. Ada kemungkinan bahwa interaksi ini penting untuk fungsi protein secara benar. Mutan V48A tidak banyak mempengaruhi stabilitas dari dihydropteroate synthase M. leprae. Kesimpulan: Berdasarkan analisis molecular docking, mutasi V48A tidak mempengaruhi binding affinity dapson terhadap dihydropteroate synthase M. leprae. Hasil ini menunjukkan mutan V48A kemungkinan tetaprentan terhadap dapson. Dengan demikian perlu dilakukan uji in vivo untuk mengkofirmasi efek mutasi V48A. Kata kunci: Mycobacterium leprae, folP1 gene, dihydropteroate synthase, dapson Abstract Background: Leprosy is a disease caused by Mycobacterium leprae. Drug resistance is one of the challenges in leprosy elimination especially in Papua. The presence of mutations in folP1 gene that encode dihydropteroate synthase (DHPS) was considered as the exclusive basis for molecular detection of dapsone resistance in leprosy. The objective of this study was to detect mutations in the folP1 gene of Mycobacterium leprae from Papua, Indonesia and to analyze the effect of these mutations on dapsone using the in-silico method. Methods: Identification of mutations in the folp1 M. leprae gene is carried out through the Basic Local Alignment Search Tool (BLAST) process in the gene bank. The analysis of the effects of mutations using the Have (y)Our Protein Explained (HOPE) server. Bindings pocket prediction is done using the Computed Atlas of Surface Topography of proteins (CASTp). Homology modeling 3D structure of DHPS using the Iterative Threading ASSEmbly Refinement (I-TASSER) server. Docking analysis was performed using AutoDock Vina which is integrated with the Python Prescription (PyRx)
{"title":"In silico analysis of V48A dihydropteroate synthase mutation to dapsone on Mycobacterium leprae from Papua","authors":"Y. Maladan, H. Krismawati, H. Hutapea, A. Oktavian","doi":"10.22435/hsji.v11i2.3744","DOIUrl":"https://doi.org/10.22435/hsji.v11i2.3744","url":null,"abstract":"Latar belakang: Lepra merupakan penyakit yang disebabkan oleh Mycobacterium leprae. Resistensi obat merupakan salah satu tantangan dalam pemberantasan kusta khususnya di Papua. Adanya mutasi pada gen folP1 penyandi dihydropteroate synthase (DHPS) merupakan dasar untuk deteksi molekuler resistensi dapson pada penyakit lepra. Tujuan penelitian ini adalah mendeteksi mutasi pada gen folP1 Mycobacterium leprae dari Papua, Indonesia dan menganalisis pengaruh mutasi tersebut terhadap dapson dengan metode in silico. \u0000Metode: Identifikasi mutasi pada gen folp1 M. leprae dilakukan melalui proses Basic Local Alignment Search Tool (BLAST) di gene bank. Analisis efek mutasi dengan menggunakan server Have (y) Our Protein Explained (HOPE). Prediksi binding pocket menggunakan Computed Atlas of Surface Topography of proteins (CASTp). Homologi modeling struktur 3D DHPS menggunakan server Iterative Threading ASSEmbly Refinement (I TASSER). Analisis docking dengan menggunakan AutoDock Vina yang terintegrasi dengan aplikasi Python Prescription (PyRx). \u0000Hasil: Hasil sekuensing menunjukkan adanya variasi dalam gen folP1 M. leprae yaitu perubahan dari Timin (T) menjadi Sitosin (C) pada nukleotida 143. Residu yang bermutasi (V48A) terletak pada domain yang penting untuk aktivitas protein dan kontak dengan residu di domain lain. Ada kemungkinan bahwa interaksi ini penting untuk fungsi protein secara benar. Mutan V48A tidak banyak mempengaruhi stabilitas dari dihydropteroate synthase M. leprae. \u0000Kesimpulan: Berdasarkan analisis molecular docking, mutasi V48A tidak mempengaruhi binding affinity dapson terhadap dihydropteroate synthase M. leprae. Hasil ini menunjukkan mutan V48A kemungkinan tetaprentan terhadap dapson. Dengan demikian perlu dilakukan uji in vivo untuk mengkofirmasi efek mutasi V48A. \u0000Kata kunci: Mycobacterium leprae, folP1 gene, dihydropteroate synthase, dapson \u0000 \u0000Abstract \u0000Background: Leprosy is a disease caused by Mycobacterium leprae. Drug resistance is one of the challenges in leprosy elimination especially in Papua. The presence of mutations in folP1 gene that encode dihydropteroate synthase (DHPS) was considered as the exclusive basis for molecular detection of dapsone resistance in leprosy. The objective of this study was to detect mutations in the folP1 gene of Mycobacterium leprae from Papua, Indonesia and to analyze the effect of these mutations on dapsone using the in-silico method. \u0000Methods: Identification of mutations in the folp1 M. leprae gene is carried out through the Basic Local Alignment Search Tool (BLAST) process in the gene bank. The analysis of the effects of mutations using the Have (y)Our Protein Explained (HOPE) server. Bindings pocket prediction is done using the Computed Atlas of Surface Topography of proteins (CASTp). Homology modeling 3D structure of DHPS using the Iterative Threading ASSEmbly Refinement (I-TASSER) server. Docking analysis was performed using AutoDock Vina which is integrated with the Python Prescription (PyRx)","PeriodicalId":30666,"journal":{"name":"Health Science Journal of Indonesia","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44102650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-18DOI: 10.22435/hsji.v11i2.2349
Suyatmi Suyatmi, Endang Listyaningsih Suparyanti, R. Pesik
Latar Belakang: Tingginya insidensi kanker di Indonesia, termasuk kanker prostat menimbulkan beban ekonomi kesehatan yang tinggi bagi Indonesia. Pengembangan terapi kanker berbasis sumber daya alam lokal dapat membantu meringankan beban negara. Penelitian ini bertujuan untuk mengetahui potensi aktivitas anti-kanker fraksi ethyl acetat Caesalpinia sappan L. terhadap sel line kanker PC3 yang merupakan model in vitro kanker prostat. Metode: Fraksi ethyl acetat kayu secang (Caesalpinia sappan L.) diperoleh melalui proses liquid chromatography. Efek Fraksi 9 dari Fraksi ethyl acetat kayu secang terhadap aktivitas anti-proliferasi dan migrasi sel diuji menggunakan desain uji in vitro. Hambatan proliferasi sel diukur dengan metode MTT assay, sedangkan aktivitas migrasi sel diukur dengan metode migration assay Hasil: Fraksi 9 dari Fraksi Ethyl acetat kayu secang memperlihatkan hambatan proliferasi sel line kanker PC3 dengan IC50:14.99μg/ml. Hasil migration assay menunjukkan pada dosis 10μg/ml Fraksi 9 menghambat migrasi sel line kanker PC3, sedangkan pada dosis 100μg/ml sel line kanker PC3 mati. Kesimpulan: Fraksi 9 dari fraksi Ethyl acetat kayu secang menunjukkan aktifitas anti-proliferasi dan anti-migrasi yang kuat terhadap pertumbuhan sel line kanker PC3 secara in vitro. Kata kunci : Ethyl Acetat fraction, Caesalpinia sappan, prostate cancer, PC3, migrasi sel Abstract Background: The high incidence of cancer, including prostate cancer, in Indonesia create a high burden on health economic cost. Development of cancer therapy based on local natural resources may help the country to alleviate the burden. This research aimed to find out the potency of selected compound of Ethyl Acetate fractions of Caesalpinia sappan as anti-cancer by using PC3 cancer cell line as an in vitro model of prostate cancer. Methods: Ethyl Acetate fraction of Caesalpinia sappan L.heartwood was prepared using a liquid chromatography method. The effect of Ethyl acetate fraction 9 on anti-proliferative and cell migration activities was assessed using MTT assay and migration assay. Results: Fraction-9 of Ethyl Acetate fraction of Caesalpinia sappan L. wood showed inhibition of PC3 cancer cell line proliferation. The IC50 of the fraction was 14.99μg/ml. The migration assay showed inhibition of cell migration on dose 10μg/ml compared to the 0 doses, while most of the cells cultured was dead when treated with 100μg/ml Fraction 9. Conclusion: Ethyl Acetate fraction 9 of Caesalpinia sappan L heartwood possibly has anti-cancer properties based on its anti-proliferative and anti-migration activities against PC3 cancer cell line. Keywords: Ethyl Acetate fraction, Caesalpinia sappan, prostate cancer, PC3, cell migration
{"title":"The effect of ethyl acetat fraction of Caesalpinia sappan L. wood on PC3 cancer cell line : cell viability and migration study","authors":"Suyatmi Suyatmi, Endang Listyaningsih Suparyanti, R. Pesik","doi":"10.22435/hsji.v11i2.2349","DOIUrl":"https://doi.org/10.22435/hsji.v11i2.2349","url":null,"abstract":"Latar Belakang: Tingginya insidensi kanker di Indonesia, termasuk kanker prostat menimbulkan beban ekonomi kesehatan yang tinggi bagi Indonesia. Pengembangan terapi kanker berbasis sumber daya alam lokal dapat membantu meringankan beban negara. Penelitian ini bertujuan untuk mengetahui potensi aktivitas anti-kanker fraksi ethyl acetat Caesalpinia sappan L. terhadap sel line kanker PC3 yang merupakan model in vitro kanker prostat. \u0000Metode: Fraksi ethyl acetat kayu secang (Caesalpinia sappan L.) diperoleh melalui proses liquid chromatography. Efek Fraksi 9 dari Fraksi ethyl acetat kayu secang terhadap aktivitas anti-proliferasi dan migrasi sel diuji menggunakan desain uji in vitro. Hambatan proliferasi sel diukur dengan metode MTT assay, sedangkan aktivitas migrasi sel diukur dengan metode migration assay \u0000Hasil: Fraksi 9 dari Fraksi Ethyl acetat kayu secang memperlihatkan hambatan proliferasi sel line kanker PC3 dengan IC50:14.99μg/ml. Hasil migration assay menunjukkan pada dosis 10μg/ml Fraksi 9 menghambat migrasi sel line kanker PC3, sedangkan pada dosis 100μg/ml sel line kanker PC3 mati. \u0000Kesimpulan: Fraksi 9 dari fraksi Ethyl acetat kayu secang menunjukkan aktifitas anti-proliferasi dan anti-migrasi yang kuat terhadap pertumbuhan sel line kanker PC3 secara in vitro. \u0000Kata kunci : Ethyl Acetat fraction, Caesalpinia sappan, prostate cancer, PC3, migrasi sel \u0000 \u0000Abstract \u0000Background: The high incidence of cancer, including prostate cancer, in Indonesia create a high burden on health economic cost. Development of cancer therapy based on local natural resources may help the country to alleviate the burden. This research aimed to find out the potency of selected compound of Ethyl Acetate fractions of Caesalpinia sappan as anti-cancer by using PC3 cancer cell line as an in vitro model of prostate cancer. \u0000Methods: Ethyl Acetate fraction of Caesalpinia sappan L.heartwood was prepared using a liquid chromatography method. The effect of Ethyl acetate fraction 9 on anti-proliferative and cell migration activities was assessed using MTT assay and migration assay. \u0000Results: Fraction-9 of Ethyl Acetate fraction of Caesalpinia sappan L. wood showed inhibition of PC3 cancer cell line proliferation. The IC50 of the fraction was 14.99μg/ml. The migration assay showed inhibition of cell migration on dose 10μg/ml compared to the 0 doses, while most of the cells cultured was dead when treated with 100μg/ml Fraction 9. \u0000Conclusion: Ethyl Acetate fraction 9 of Caesalpinia sappan L heartwood possibly has anti-cancer properties based on its anti-proliferative and anti-migration activities against PC3 cancer cell line. \u0000Keywords: Ethyl Acetate fraction, Caesalpinia sappan, prostate cancer, PC3, cell migration","PeriodicalId":30666,"journal":{"name":"Health Science Journal of Indonesia","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47973145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-18DOI: 10.22435/hsji.v11i2.3749
Farida Dwi Handayani, Rahmi Ayu Wijayaningsih, M. H. Gasem, T. Wibawa
Latar belakang: Leptospirosis merupakan zoonosis penting di dunia, yang masih sering terjadi salah diagnosis. Deteksi laboratorium Leptospira menjadi tantangan karena bakterimea cukup singkat untuk dideteksi molekuler, namun antibodi juga muncul sangat lambat. Urine dapat menjadi sampel alternatif untuk deteksi PCR pada leptospirosis. Pengerjaan PCR membutuhkan DNA berkualitas dan andal, dan diperoleh dari metode ekstraksi DNA yang baik. Penelitian bertujuan untuk mengetahui metode ekstraksi DNA Leptospira terbaik untuk sampel urin, serta mengevaluasi pengaruh waktu penyimpanan dan suhu terhadap kestabilan DNA. Metode: Penelitian ini menggunakan tiga metode isolasi DNA yang berbeda; berbasis silika dengan spin kolom, kromatografi spin column menggunakan resin sebagai matriks pemisah, dan metode larutan dengan guanidine isothiocyanate. Hasil ekstraksi diperiksa konsentrasi dan kemurniannya. Gen SecY pada Leptospira dideteksi dengan PCR real-time. Pengaruh suhu dan lama penyimpanan DNA juga dilihat. Hasil: Hasil isolasi DNA menggunakan resin menunjukkan konsentrasi tertinggi (7,94 + 2,11 μg / mL) dan jumlah salinan amplifikasi DNA Leptospira tertinggi (50167,92 + 1,19). Suhu penyimpanan pada suhu 4°C, -20°C, dan -80°C dan umur simpan 91 hari tidak berpengaruh terhadap kualitas dan kuantitas DNA Leptospira hasil isolasi spike urin. Kesimpulan: Isolasi DNA menggunakan spin column chromatography dengan resin sebagai matriks separasi memiliki kualitas dan kuantitas terbaik berdasarkan kemurnian dan konsentrasi DNA serta jumlah gen SecY yang teramplifikasi. Kata kunci: Leptospira, Leptospirosis, ekstraksi DNA, sampel urin, penyimpanan sampel. Abstract Background: Leptospirosis is a worldwide zoonotic disease, which is still often misdiagnosed. Laboratory detection of Leptospira is challenging since the bacteraemia is quite short for molecular detection, however, the rise of the antibody is late to post the infection. Urine can be a potential alternative sample for PCR detection in leptospirosis. The PCR method requires a reliable DNA template, which is obtained from good DNA extracting methods. The study aimed to determine the best method of extraction Leptospira DNA from the urine sample, as well as evaluating the effect of time storage and temperature for its DNA stability. Methods: This study was utilizing three different DNA isolation methods; silica based with spin column, spin column chromatography using resin as separation matrix, and solution method with guanidine isothiocyanate. The yields were examined for its concentration and purity. Leptospira’s SecY gene was detected with realtime PCR. The influences of storage temperature and the life time of the DNA were also studied. Results: The yield of DNA isolation using resin showed the highest concentration (7.94+2.11 μg/mL) and highest Leptospira DNA amplification copy number (50167.92+1.19). Storage temperature at 4°C, -20°C, and -80°C and life time of 91 days did not have any effect
{"title":"Comparison of DNA extraction methods for molecular identification of pathogenic Leptospira in the urine samples","authors":"Farida Dwi Handayani, Rahmi Ayu Wijayaningsih, M. H. Gasem, T. Wibawa","doi":"10.22435/hsji.v11i2.3749","DOIUrl":"https://doi.org/10.22435/hsji.v11i2.3749","url":null,"abstract":"Latar belakang: Leptospirosis merupakan zoonosis penting di dunia, yang masih sering terjadi salah diagnosis. Deteksi laboratorium Leptospira menjadi tantangan karena bakterimea cukup singkat untuk dideteksi molekuler, namun antibodi juga muncul sangat lambat. Urine dapat menjadi sampel alternatif untuk deteksi PCR pada leptospirosis. Pengerjaan PCR membutuhkan DNA berkualitas dan andal, dan diperoleh dari metode ekstraksi DNA yang baik. Penelitian bertujuan untuk mengetahui metode ekstraksi DNA Leptospira terbaik untuk sampel urin, serta mengevaluasi pengaruh waktu penyimpanan dan suhu terhadap kestabilan DNA. \u0000Metode: Penelitian ini menggunakan tiga metode isolasi DNA yang berbeda; berbasis silika dengan spin kolom, kromatografi spin column menggunakan resin sebagai matriks pemisah, dan metode larutan dengan guanidine isothiocyanate. Hasil ekstraksi diperiksa konsentrasi dan kemurniannya. Gen SecY pada Leptospira dideteksi dengan PCR real-time. Pengaruh suhu dan lama penyimpanan DNA juga dilihat. \u0000Hasil: Hasil isolasi DNA menggunakan resin menunjukkan konsentrasi tertinggi (7,94 + 2,11 μg / mL) dan jumlah salinan amplifikasi DNA Leptospira tertinggi (50167,92 + 1,19). Suhu penyimpanan pada suhu 4°C, -20°C, dan -80°C dan umur simpan 91 hari tidak berpengaruh terhadap kualitas dan kuantitas DNA Leptospira hasil isolasi spike urin. \u0000Kesimpulan: Isolasi DNA menggunakan spin column chromatography dengan resin sebagai matriks separasi memiliki kualitas dan kuantitas terbaik berdasarkan kemurnian dan konsentrasi DNA serta jumlah gen SecY yang teramplifikasi. \u0000Kata kunci: Leptospira, Leptospirosis, ekstraksi DNA, sampel urin, penyimpanan sampel. \u0000 \u0000Abstract \u0000Background: Leptospirosis is a worldwide zoonotic disease, which is still often misdiagnosed. Laboratory detection of Leptospira is challenging since the bacteraemia is quite short for molecular detection, however, the rise of the antibody is late to post the infection. Urine can be a potential alternative sample for PCR detection in leptospirosis. The PCR method requires a reliable DNA template, which is obtained from good DNA extracting methods. The study aimed to determine the best method of extraction Leptospira DNA from the urine sample, as well as evaluating the effect of time storage and temperature for its DNA stability. \u0000Methods: This study was utilizing three different DNA isolation methods; silica based with spin column, spin column chromatography using resin as separation matrix, and solution method with guanidine isothiocyanate. The yields were examined for its concentration and purity. Leptospira’s SecY gene was detected with realtime PCR. The influences of storage temperature and the life time of the DNA were also studied. \u0000Results: The yield of DNA isolation using resin showed the highest concentration (7.94+2.11 μg/mL) and highest Leptospira DNA amplification copy number (50167.92+1.19). Storage temperature at 4°C, -20°C, and -80°C and life time of 91 days did not have any effect ","PeriodicalId":30666,"journal":{"name":"Health Science Journal of Indonesia","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42449376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-18DOI: 10.22435/hsji.v11i2.3952
Rachmasari Putri, Sari Eka Pratiwi, D. S. Heriyanto, Danarto Danarto, I. Astuti, N. Arfian, S. Haryana
Latar Belakang: Gangguan regulasi mikroRNA(miR) dan inflamasi kronik dapat mengubah tumor menjadi karsinoma dan kanker dengan metastasis melalui perubahan seluler dan genomik. Lesi prekanker memiliki peluang 33,3 persen menjadi kanker. Penelitian ini bertujuan untuk mengkaji peran miR-155-5p terhadap mRNA SOCS1 dan populasi makrofag terhadap progresivitas penyakit yang berhubungan dengan Benign Prostate Hyperplasia (BPH), High Grade Prostatic Intraepithelial Neoplasia (HGPIN), dan Prostate Adenocarcinoma (PRAD). Metode: Penelitian ini merupakan penelitian potong lintang dengan 3 kelompok, yaitu BPH,HGPIN, dan PRAD. Sampel jaringan didapatkan dari Tindakan TURP. Ekspresi miR-155 dianalisis menggunakan qPCR dan dikalkulasi menggunakan metode Livak. Ekspresi mRNA SOCS-1 dianalisis menggunakan reverse transcriptase PCR. Penanda pan makrofag, anti CD-68 monoclonal antibody(MoAb) digunakan untuk mendeteksi populasi makrofag pada jaringan dengan imunohistokimia. Hasil: Ekspresi miR-155 lebih tinggi pada HGPIN dibandingkan BPH dan PRAD (p=0,14). Ekspresi mRNA SOCS1 pada HGPIN paling rendah diantara ketiga sampel (p=0,96). Terdapat korelasi negative antara miR-155 dan mRNA SOCS1 (p=0,02). Terdapat peningkatan persentase populasi makrofag yang signifikan pada HGPIN (6,03 persen) dibandingkan BPH (0.89 persen) dengan p=0,00. Kesimpulan: Pada penelitian ini, terdapat perubahan persentase makrofag dan miR-155 pada HGPIN. Variasi ekspresi miR-155 dan persentase populasi makrofag dapat disebabkan karena perubahan epigenetik. Oleh sebab itu, perlu penelitian lebih lanjut untuk memvalidasi hasil tersebut dan memahami kemungkinan menjadi biomarker pada penyakit prekanker pada prostat. Kata Kunci: Prostatic Intaepithelial Neoplasia, miR-155, Makrofag Abstract Background: Impaired microRNA(miR) regulation and chronic inflammation could transform tumors into carcinoma and cancer by metastasis through cellular and genomic changes. Precancerous lesions have a 33.3 percent chance of becoming cancerous. This study investigated the role of miR-155 related to SOCS1 mRNA and macrophage population in disease progression associated with Benign Prostate Hyperplasia (BPH), High-Grade Prostatic Intraepithelial Neoplasia (HGPIN), and Prostate Adenocarcinoma (PRAD). Methods: This was a cross-sectional study using three groups of samples, namely BPH, HGPIN, and PRAD. Tissue samples were obtained from TURP Action. The expression of miR-155 was analyzed using real-time qPCR and calculated using the Livak method. The expression of SOCS1 mRNA was analyzed using reverse transcriptase PCR. The macrophage pan-marker, anti-CD68 monoclonal antibody (MoAb), was used to detect macrophage population in tissues by immunohistochemistry. Results: The expression of miR-155 was higher in HGPIN than BPH and PRAD (p=0.14). The expression of SOCS1 mRNA in HGPIN was the lowest among the three samples (p=0.96). There was a negative correlation between miR-155 and SOCS1 mRNA (p=0.02). The
{"title":"Overexpression of MiR-155-5p and increased number of macrophage population in precancerous prostatic disease","authors":"Rachmasari Putri, Sari Eka Pratiwi, D. S. Heriyanto, Danarto Danarto, I. Astuti, N. Arfian, S. Haryana","doi":"10.22435/hsji.v11i2.3952","DOIUrl":"https://doi.org/10.22435/hsji.v11i2.3952","url":null,"abstract":"Latar Belakang: Gangguan regulasi mikroRNA(miR) dan inflamasi kronik dapat mengubah tumor menjadi karsinoma dan kanker dengan metastasis melalui perubahan seluler dan genomik. Lesi prekanker memiliki peluang 33,3 persen menjadi kanker. Penelitian ini bertujuan untuk mengkaji peran miR-155-5p terhadap mRNA SOCS1 dan populasi makrofag terhadap progresivitas penyakit yang berhubungan dengan Benign Prostate Hyperplasia (BPH), High Grade Prostatic Intraepithelial Neoplasia (HGPIN), dan Prostate Adenocarcinoma (PRAD). \u0000Metode: Penelitian ini merupakan penelitian potong lintang dengan 3 kelompok, yaitu BPH,HGPIN, dan PRAD. Sampel jaringan didapatkan dari Tindakan TURP. Ekspresi miR-155 dianalisis menggunakan qPCR dan dikalkulasi menggunakan metode Livak. Ekspresi mRNA SOCS-1 dianalisis menggunakan reverse transcriptase PCR. Penanda pan makrofag, anti CD-68 monoclonal antibody(MoAb) digunakan untuk mendeteksi populasi makrofag pada jaringan dengan imunohistokimia. \u0000Hasil: Ekspresi miR-155 lebih tinggi pada HGPIN dibandingkan BPH dan PRAD (p=0,14). Ekspresi mRNA SOCS1 pada HGPIN paling rendah diantara ketiga sampel (p=0,96). Terdapat korelasi negative antara miR-155 dan mRNA SOCS1 (p=0,02). Terdapat peningkatan persentase populasi makrofag yang signifikan pada HGPIN (6,03 persen) dibandingkan BPH (0.89 persen) dengan p=0,00. \u0000Kesimpulan: Pada penelitian ini, terdapat perubahan persentase makrofag dan miR-155 pada HGPIN. Variasi ekspresi miR-155 dan persentase populasi makrofag dapat disebabkan karena perubahan epigenetik. Oleh sebab itu, perlu penelitian lebih lanjut untuk memvalidasi hasil tersebut dan memahami kemungkinan menjadi biomarker pada penyakit prekanker pada prostat. \u0000Kata Kunci: Prostatic Intaepithelial Neoplasia, miR-155, Makrofag \u0000 \u0000Abstract \u0000 \u0000Background: Impaired microRNA(miR) regulation and chronic inflammation could transform tumors into carcinoma and cancer by metastasis through cellular and genomic changes. Precancerous lesions have a 33.3 percent chance of becoming cancerous. This study investigated the role of miR-155 related to SOCS1 mRNA and macrophage population in disease progression associated with Benign Prostate Hyperplasia (BPH), High-Grade Prostatic Intraepithelial Neoplasia (HGPIN), and Prostate Adenocarcinoma (PRAD). \u0000Methods: This was a cross-sectional study using three groups of samples, namely BPH, HGPIN, and PRAD. Tissue samples were obtained from TURP Action. The expression of miR-155 was analyzed using real-time qPCR and calculated using the Livak method. The expression of SOCS1 mRNA was analyzed using reverse transcriptase PCR. The macrophage pan-marker, anti-CD68 monoclonal antibody (MoAb), was used to detect macrophage population in tissues by immunohistochemistry. \u0000Results: The expression of miR-155 was higher in HGPIN than BPH and PRAD (p=0.14). The expression of SOCS1 mRNA in HGPIN was the lowest among the three samples (p=0.96). There was a negative correlation between miR-155 and SOCS1 mRNA (p=0.02). The","PeriodicalId":30666,"journal":{"name":"Health Science Journal of Indonesia","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41630836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-18DOI: 10.22435/hsji.v11i2.3149
S. N. Wahyuningrum, C. Prihharsanti, S. Haryana, A. Ghozali, Firly Putri Fardhila
Latar belakang: Angka kekambuhan dan resistensi pasien kanker rektum mencapai 40 persen. Kondisi tersebut bisa disebabkan karena peningkatan ekspresi GLUT-1 dan VEGF-A, serta mempengaruhi prognosis pasien. Tujuan penelitian ini adalah untuk mengetahui korelasi ekspresi mRNA GLUT-1 dan VEGF-A, serta hubungannya dengan prognosis pasien kanker rektum yang menjalani kemoradiasi. Metode: Penelitian Kohor ini melibatkan 16 orang pasien kanker rektum lokal stadium II atau III yang menjalani kemoradiasi di RSUP Kariadi Semarang. Sampel darah intravena diambil 5 mL pada saat sebelum dan sesudah kemoradiasi. Total RNA diisolasi dari 200 μl serum, kemudian dilakukan sintesis cDNA. Ekspresi mRNA GLUT-1 dan VEGF-A dikuantifikasi dengan metode Livak menggunakan reference gene β-actin. Hasil: Rata-rata ekspresi mRNA GLUT-1 menurun signifikan 2,14 kali (P=0,044) dan mRNA VEGF-A menurun 1,9 kali (P = 0,03). Ekspresi mRNA GLUT-1 dan VEGF-A berkorelasi positif kuat dan signifikan pada saat sebelum (r = 0,6; R2 = 0,455; P = 0,013) dan sesudah kemoradiasi (r = 0,8; R2 = 0,598; P < 0,001). Peningkatan ekspresi mRNA GLUT-1 berhubungan dengan prognosis buruk pasien, dengan resiko 18 kali lebih tinggi (P=0,036; OR=18, 95% CI=1,2 – 261). Peningkatan ekspresi mRNA VEGF-A tidak berhubungan signifikan dengan prognosis pasien (P=0,12; OR=9; 95%CI=0,6-123). Kesimpulan: Ekspresi mRNA GLUT-1 dan VEGF-A berkorelasi positif dan saling mempengaruhi satu dengan lainnya. Peningkatan ekspresi mRNA GLUT-1 berhubungan dengan prognosis buruk pasien. Hasil penelitian ini mengindikasikan bahwa ekspresi mRNA GLUT-1 dari sampel darah berpotensi sebagai biomarker prognosis pada pasien kanker rektum yang menjalani kemoradiasi. Kata kunci: kanker rektum, kemoradiasi, GLUT-1, VEGF-A, prognosis Abstract Background: Rectal cancer patients have 40 percent risk of recurrence and resistance, which is triggered by increasing in GLUT-1 and VEGF-A mRNA expression. This condition associate with the patients prognosis. This study aimed to determine the correlation between GLUT-1 and VEGF-A mRNA expression, and analyze its association with the rectal cancer patients prognosis who received chemoradiation. Methods: This was a Cohort study involving 16 rectal cancer patients with stage II or III undergoing chemoradiation at Kariadi Hospital Semarang. Five milliliters of intravenous blood samples were taken before and after chemoradiation. Total RNA was isolated from 200 μl of blood serum, followed by cDNA synthesis. GLUT-1 and VEGF-A mRNA expression was quantified by the Livak method using β-actin as a reference gene. Results: GLUT-1 and VEGF-A mRNA expression decreased significantly 2.14 times (P=0,044) and 1,9 times (P=0,03), respectively. Expression of GLUT-1 and VEGF-A mRNA have a significant and strong positive correlation at before (r=0,6; R2=0,455; P=0,013) and after chemoradiation (r=0,8; R2=0,598; P<0,001). GLUT-1 mRNA expression enhancement significantly associate with poor prognosis and ris
{"title":"The expression of GLUT-1 and VEGF-A mRNA in the rectal cancer patients with neoadjuvant chemoradiation","authors":"S. N. Wahyuningrum, C. Prihharsanti, S. Haryana, A. Ghozali, Firly Putri Fardhila","doi":"10.22435/hsji.v11i2.3149","DOIUrl":"https://doi.org/10.22435/hsji.v11i2.3149","url":null,"abstract":"Latar belakang: Angka kekambuhan dan resistensi pasien kanker rektum mencapai 40 persen. Kondisi tersebut bisa disebabkan karena peningkatan ekspresi GLUT-1 dan VEGF-A, serta mempengaruhi prognosis pasien. Tujuan penelitian ini adalah untuk mengetahui korelasi ekspresi mRNA GLUT-1 dan VEGF-A, serta hubungannya dengan prognosis pasien kanker rektum yang menjalani kemoradiasi. \u0000Metode: Penelitian Kohor ini melibatkan 16 orang pasien kanker rektum lokal stadium II atau III yang menjalani kemoradiasi di RSUP Kariadi Semarang. Sampel darah intravena diambil 5 mL pada saat sebelum dan sesudah kemoradiasi. Total RNA diisolasi dari 200 μl serum, kemudian dilakukan sintesis cDNA. Ekspresi mRNA GLUT-1 dan VEGF-A dikuantifikasi dengan metode Livak menggunakan reference gene β-actin. \u0000Hasil: Rata-rata ekspresi mRNA GLUT-1 menurun signifikan 2,14 kali (P=0,044) dan mRNA VEGF-A menurun 1,9 kali (P = 0,03). Ekspresi mRNA GLUT-1 dan VEGF-A berkorelasi positif kuat dan signifikan pada saat sebelum (r = 0,6; R2 = 0,455; P = 0,013) dan sesudah kemoradiasi (r = 0,8; R2 = 0,598; P < 0,001). Peningkatan ekspresi mRNA GLUT-1 berhubungan dengan prognosis buruk pasien, dengan resiko 18 kali lebih tinggi (P=0,036; OR=18, 95% CI=1,2 – 261). Peningkatan ekspresi mRNA VEGF-A tidak berhubungan signifikan dengan prognosis pasien (P=0,12; OR=9; 95%CI=0,6-123). \u0000Kesimpulan: Ekspresi mRNA GLUT-1 dan VEGF-A berkorelasi positif dan saling mempengaruhi satu dengan lainnya. Peningkatan ekspresi mRNA GLUT-1 berhubungan dengan prognosis buruk pasien. Hasil penelitian ini mengindikasikan bahwa ekspresi mRNA GLUT-1 dari sampel darah berpotensi sebagai biomarker prognosis pada pasien kanker rektum yang menjalani kemoradiasi. \u0000Kata kunci: kanker rektum, kemoradiasi, GLUT-1, VEGF-A, prognosis \u0000Abstract \u0000Background: Rectal cancer patients have 40 percent risk of recurrence and resistance, which is triggered by increasing in GLUT-1 and VEGF-A mRNA expression. This condition associate with the patients prognosis. This study aimed to determine the correlation between GLUT-1 and VEGF-A mRNA expression, and analyze its association with the rectal cancer patients prognosis who received chemoradiation. \u0000Methods: This was a Cohort study involving 16 rectal cancer patients with stage II or III undergoing chemoradiation at Kariadi Hospital Semarang. Five milliliters of intravenous blood samples were taken before and after chemoradiation. Total RNA was isolated from 200 μl of blood serum, followed by cDNA synthesis. GLUT-1 and VEGF-A mRNA expression was quantified by the Livak method using β-actin as a reference gene. \u0000Results: GLUT-1 and VEGF-A mRNA expression decreased significantly 2.14 times (P=0,044) and 1,9 times (P=0,03), respectively. Expression of GLUT-1 and VEGF-A mRNA have a significant and strong positive correlation at before (r=0,6; R2=0,455; P=0,013) and after chemoradiation (r=0,8; R2=0,598; P<0,001). GLUT-1 mRNA expression enhancement significantly associate with poor prognosis and ris","PeriodicalId":30666,"journal":{"name":"Health Science Journal of Indonesia","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49117739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-06-30DOI: 10.22435/hsji.v11i1.3127
L. Moehario, E. Tjoa, M. J. Taslim, Yohanna Angelina
Background: Legionella pneumophila (L. pneumophila) has been known as the etiology of legionellosis; they live in aquatic environment, warm and moist. Culture method using specific medium remains as the gold standard in the identification of L. pneumophila. This study aimed to compare the recovery rate of L. pneumophila ATCC® 33823 on the specific medium BCYE for the cultivation of Legionella, and BMPA, the selective medium. Methods: Suspension of L. pneumophila ATCC® 33823 of 0.5 McFarland was diluted to 10 fold serial dilution; 100 ul of each dilution was inoculated on Buffered Charcoal Yeast Extract (BCYE) medium, and BMPA (BCYE supplemented with BMPA-α) in duplicate manner. The concentration was calculated using Total Plate Count standard as of Indonesian Nasional Standard number 01-2332.3-2006. The percentage of recovery rate was calculated, and the statistical analysis was performed using SPSS version 23.0. Results: Numbers of colonies of L. pneumophila grew on BMPA was much higher than on BCYE medium; the highest concentration was yielded on BMPA medium i.e. 1.45x107 CFU/ml. The recovery rates were 96.67% and 60.67% on BMPA medium and BCYE subsequently. Conclusion: The recovery rate of the BMPA medium on the colony growth of L. pneumophila ATCC®33823 was markedly higher than the BCYE, therefore BMPA medium can be suggested to be used in the cultivation of L. pneumophila especially in the routine surveillance program for water sources with less cost. (Health Science Journal of Indonesia 2020;11(1):32-7)
{"title":"Comparison of BCYE and BMPA media on recovery rate of Legionella pneumophila","authors":"L. Moehario, E. Tjoa, M. J. Taslim, Yohanna Angelina","doi":"10.22435/hsji.v11i1.3127","DOIUrl":"https://doi.org/10.22435/hsji.v11i1.3127","url":null,"abstract":"Background: Legionella pneumophila (L. pneumophila) has been known as the etiology of legionellosis; they live in aquatic environment, warm and moist. Culture method using specific medium remains as the gold standard in the identification of L. pneumophila. This study aimed to compare the recovery rate of L. pneumophila ATCC® 33823 on the specific medium BCYE for the cultivation of Legionella, and BMPA, the selective medium. Methods: Suspension of L. pneumophila ATCC® 33823 of 0.5 McFarland was diluted to 10 fold serial dilution; 100 ul of each dilution was inoculated on Buffered Charcoal Yeast Extract (BCYE) medium, and BMPA (BCYE supplemented with BMPA-α) in duplicate manner. The concentration was calculated using Total Plate Count standard as of Indonesian Nasional Standard number 01-2332.3-2006. The percentage of recovery rate was calculated, and the statistical analysis was performed using SPSS version 23.0. Results: Numbers of colonies of L. pneumophila grew on BMPA was much higher than on BCYE medium; the highest concentration was yielded on BMPA medium i.e. 1.45x107 CFU/ml. The recovery rates were 96.67% and 60.67% on BMPA medium and BCYE subsequently. Conclusion: The recovery rate of the BMPA medium on the colony growth of L. pneumophila ATCC®33823 was markedly higher than the BCYE, therefore BMPA medium can be suggested to be used in the cultivation of L. pneumophila especially in the routine surveillance program for water sources with less cost. (Health Science Journal of Indonesia 2020;11(1):32-7)","PeriodicalId":30666,"journal":{"name":"Health Science Journal of Indonesia","volume":"11 1","pages":"32-37"},"PeriodicalIF":0.0,"publicationDate":"2020-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45199470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-06-30DOI: 10.22435/hsji.v11i1.3129
A. Elisanti, Diany Yoke Safira, E. T. Ardianto
Background: Tuberculosis has become a global health problem, included in Indonesia, new cases of pulmonary TB increase continuously. This study examined the dominant factors that influenced the transmission of household contacts in pulmonary TB patients. Methods: This observational study was a cross-sectional analytic design which used a population of all patients and family members of pulmonary TB patients at the Kedundung Health Center in 2015 until 2016. A sample of 52 people was counted using the infinith sample size formula and taken consecutively. Research variables include prevention behavior, AFB smear gradation, and household contact transmission. Collecting data used interview sheets, data collection sheets, sterile sputum pots. Data was analyzed by chi-square test and binary logistic regression test with a significance level of 0.05. Results: Prevention behavior (p=0.093), AFB smear-positive 2+ (p=0.377) did not affect on household contact transmission in pulmonary TB patients, whereas AFB smear-positive 1+ affected household contacts transmission of pulmonary TB patients (p=0.007). The value of Exp (B), AFB smear-positive (1+) have a risk of 12.144 times transmitting to household contact when compared to AFB smear-positive (3+). Whereas patients with AFB smear-positive (2+) have a risk of 3,328 times transmitting to household contact when compared with AFB smear-positive 3+. Conclusion: Pulmonary TB patients with AFB smear-positive (1+) was the dominant factor affecting household contact transmission. Communication and personal hygiene education efforts, the level of adherence in the treatment of pulmonary TB patients needs to be increased to suppress the new incidence of pulmonary TB. (Health Science Journal of Indonesia 2020;11(1):15-21)
{"title":"AFB smear positive 1+: a dominant factor in Pulmonary TB household transmission","authors":"A. Elisanti, Diany Yoke Safira, E. T. Ardianto","doi":"10.22435/hsji.v11i1.3129","DOIUrl":"https://doi.org/10.22435/hsji.v11i1.3129","url":null,"abstract":"Background: Tuberculosis has become a global health problem, included in Indonesia, new cases of pulmonary TB increase continuously. This study examined the dominant factors that influenced the transmission of household contacts in pulmonary TB patients. Methods: This observational study was a cross-sectional analytic design which used a population of all patients and family members of pulmonary TB patients at the Kedundung Health Center in 2015 until 2016. A sample of 52 people was counted using the infinith sample size formula and taken consecutively. Research variables include prevention behavior, AFB smear gradation, and household contact transmission. Collecting data used interview sheets, data collection sheets, sterile sputum pots. Data was analyzed by chi-square test and binary logistic regression test with a significance level of 0.05. Results: Prevention behavior (p=0.093), AFB smear-positive 2+ (p=0.377) did not affect on household contact transmission in pulmonary TB patients, whereas AFB smear-positive 1+ affected household contacts transmission of pulmonary TB patients (p=0.007). The value of Exp (B), AFB smear-positive (1+) have a risk of 12.144 times transmitting to household contact when compared to AFB smear-positive (3+). Whereas patients with AFB smear-positive (2+) have a risk of 3,328 times transmitting to household contact when compared with AFB smear-positive 3+. Conclusion: Pulmonary TB patients with AFB smear-positive (1+) was the dominant factor affecting household contact transmission. Communication and personal hygiene education efforts, the level of adherence in the treatment of pulmonary TB patients needs to be increased to suppress the new incidence of pulmonary TB. (Health Science Journal of Indonesia 2020;11(1):15-21)","PeriodicalId":30666,"journal":{"name":"Health Science Journal of Indonesia","volume":"11 1","pages":"15-21"},"PeriodicalIF":0.0,"publicationDate":"2020-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45312292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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