Pub Date : 2020-06-29DOI: 10.22435/hsji.v11i1.3068
Vita Pertiwi, Balgis Balgis, Y. Mashuri
Background: Body image is a perception of our physical appearance. Weight misperception in adolescent lead to body dissatisfaction and obesity in adolescent. Gender also plays a role in adolescent obesity and body image. Obese adolescents have greater risk of non-communicable diseases than adolescents with normal weight. therefore, this research aims to discover body image and gender influence on adolescent obesity. Method: This study is an observational design with cross sectional approach. The study was conducted in November 2019 at SMK Negeri 9 Surakarta. The subjects were 57 sophomore that were chosen randomly with simple random sampling. Body image and obesity were measured using MBSRQ-AS questionnaire, BMI for Age Charts and waist circumference. Data was processed by independent T-test, fisher exact test, logistic regression test with significance value p <0.05. Results : There is a significant difference in body image scores between obese and non obese group (p = 0.006) and male students scored higher in every aspect of body image than female students. A significant relationship was found between body image and obesity (p=0,045), and gender with obesity (p = 0.009). Conclusion: There is a significant relationship between body image and gender with obesity in adolescents and body image scores differ significantly between obese and non obese group and between male and female students. (Health Science Journal of Indonesia 2020;11(1):22-6)
背景：身体形象是对我们外表的感知。青少年对体重的错误认知导致青少年对身体的不满和肥胖。性别在青少年肥胖和身体形象方面也起着一定作用。肥胖青少年比体重正常的青少年患非传染性疾病的风险更大。因此，本研究旨在发现身体形象和性别对青少年肥胖的影响。方法：本研究采用横断面方法进行观察性设计。该研究于2019年11月在苏拉卡塔SMK Negeri 9进行。受试者为57名大二学生，采用简单随机抽样法随机抽取。使用MBSRQ-AS问卷、BMI年龄表和腰围测量身体形象和肥胖。采用独立T检验、fisher精确检验、逻辑回归检验对数据进行处理，显著性值p＜0.05。结果：肥胖组和非肥胖组的身体形象得分存在显著差异（p=0.006），男生在身体形象各方面的得分均高于女生。身体形象与肥胖（p=0.045）和性别与肥胖（p=0.009）之间存在显著相关性。结论：青少年身体形象与性别与肥胖之间存在显著关系，肥胖组和非肥胖组以及男女学生的身体形象得分差异显著。（《印度尼西亚健康科学杂志》2020；11（1）：22-6）
{"title":"The influence of body image and gender in adolescent obesity","authors":"Vita Pertiwi, Balgis Balgis, Y. Mashuri","doi":"10.22435/hsji.v11i1.3068","DOIUrl":"https://doi.org/10.22435/hsji.v11i1.3068","url":null,"abstract":"Background: Body image is a perception of our physical appearance. Weight misperception in adolescent lead to body dissatisfaction and obesity in adolescent. Gender also plays a role in adolescent obesity and body image. Obese adolescents have greater risk of non-communicable diseases than adolescents with normal weight. therefore, this research aims to discover body image and gender influence on adolescent obesity. Method: This study is an observational design with cross sectional approach. The study was conducted in November 2019 at SMK Negeri 9 Surakarta. The subjects were 57 sophomore that were chosen randomly with simple random sampling. Body image and obesity were measured using MBSRQ-AS questionnaire, BMI for Age Charts and waist circumference. Data was processed by independent T-test, fisher exact test, logistic regression test with significance value p <0.05. Results : There is a significant difference in body image scores between obese and non obese group (p = 0.006) and male students scored higher in every aspect of body image than female students. A significant relationship was found between body image and obesity (p=0,045), and gender with obesity (p = 0.009). Conclusion: There is a significant relationship between body image and gender with obesity in adolescents and body image scores differ significantly between obese and non obese group and between male and female students. (Health Science Journal of Indonesia 2020;11(1):22-6)","PeriodicalId":30666,"journal":{"name":"Health Science Journal of Indonesia","volume":"11 1","pages":"22-26"},"PeriodicalIF":0.0,"publicationDate":"2020-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44018000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-06-29DOI: 10.22435/hsji.v11i1.3150
H. Fuadzy, Mutiara Widawati, E. Astuti, Heni Prasetyowati, J. Hendri, R. W. Nurindra, Dewi Nur Hodijah
Background: Bandung have urban areas with adequate housebuilding quality, yet still has high Dengue endemic cases. This study aims to investigate the characteristics of dengue incidence at the household level. Methods: Data analyzed from 781 households consisted of 261 cases and 522 controls. We applied matched case-control samples with a ratio of 1:2 (case: controls). The stages of the research consisted of a house status observation using a form inspection checklist and interviews using a closed-ended questionnaire. The data analyzed by pairwise spearmen correlation and binary logistic regression for risk factor prediction. Results: Risk factors for productive age and low level of education of family heads, dirty toilets, and unhealthy house status have a significant effect on the increase of dengue cases in Bandung (p<0.05). The determinant factor is the productive age of the head of the family (31 60 years), it means that the family who has a family head in a productive age is 2.53 (95% CI 1.34-4.78; p <0.05) times more likely to have a household member suffering from DHF in Bandung. Conclusion: The age and the level of education of the household heads, toilet hygiene, and healthy home status have an essential role in influencing dengue fever. Therefore, health workers need to perform an intensive health promotion regarding DHF to the household heads. (Health Science Journal of Indonesia 2020;11(1):45-51)
背景:万隆的城市地区有足够的房屋建筑质量，但仍然有很高的登革热流行病例。本研究旨在调查登革热在家庭层面的发病特征。方法:对781户家庭的资料进行分析，其中病例261例，对照组522例。我们采用匹配的病例对照样本，比例为1:2(病例:对照)。研究的阶段包括使用表格检查清单进行房屋状态观察和使用封闭式问卷进行访谈。数据分析采用两两spearman相关和二元logistic回归进行危险因素预测。结果:生育年龄、户主受教育程度低、厕所脏乱、房屋状况不卫生等危险因素对万隆市登革热病例的增加有显著影响(p<0.05)。决定因素为户主的生产年龄(31 60岁)，即在生产年龄有户主的家庭为2.53 (95% CI 1.34-4.78;p <0.05)家庭成员感染登革出血热的可能性是万隆市的两倍。结论:户主年龄、文化程度、厕所卫生、家庭健康状况是影响登革热发病的重要因素。因此，卫生工作者需要就登革出血热向户主进行密集的健康宣传。(印度尼西亚卫生科学杂志2020;11(1):45-51)
{"title":"Risk factors associated with Dengue incidence in Bandung, Indonesia: a household based case-control study","authors":"H. Fuadzy, Mutiara Widawati, E. Astuti, Heni Prasetyowati, J. Hendri, R. W. Nurindra, Dewi Nur Hodijah","doi":"10.22435/hsji.v11i1.3150","DOIUrl":"https://doi.org/10.22435/hsji.v11i1.3150","url":null,"abstract":"Background: Bandung have urban areas with adequate housebuilding quality, yet still has high Dengue endemic cases. This study aims to investigate the characteristics of dengue incidence at the household level. Methods: Data analyzed from 781 households consisted of 261 cases and 522 controls. We applied matched case-control samples with a ratio of 1:2 (case: controls). The stages of the research consisted of a house status observation using a form inspection checklist and interviews using a closed-ended questionnaire. The data analyzed by pairwise spearmen correlation and binary logistic regression for risk factor prediction. Results: Risk factors for productive age and low level of education of family heads, dirty toilets, and unhealthy house status have a significant effect on the increase of dengue cases in Bandung (p<0.05). The determinant factor is the productive age of the head of the family (31 60 years), it means that the family who has a family head in a productive age is 2.53 (95% CI 1.34-4.78; p <0.05) times more likely to have a household member suffering from DHF in Bandung. Conclusion: The age and the level of education of the household heads, toilet hygiene, and healthy home status have an essential role in influencing dengue fever. Therefore, health workers need to perform an intensive health promotion regarding DHF to the household heads. (Health Science Journal of Indonesia 2020;11(1):45-51)","PeriodicalId":30666,"journal":{"name":"Health Science Journal of Indonesia","volume":"11 1","pages":"45-51"},"PeriodicalIF":0.0,"publicationDate":"2020-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43644178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-06-29DOI: 10.22435/hsji.v11i1.3143
D. Atmaka, Windi Indah Fajar Ningsih, Risnukathulistiwi Maghribi
Background: Iron deficiency may happen because of low consumption of foods rich in bioavailable iron and high consumption of foods rich in inhibitors of iron absorption. Improving dietary intake is the most effective way to break the chain of iron deficiency anemia problems. This study aimed to study the changes in dietary intake and health awareness among adolescent girl before and after iron deficiency anemia diagnosis. Method: Cohort study with 62 subjects from 2 junior high school in Wates after hemoglobin test (cyanmethemoglobin method) and were newly iron deficiency anemia diagnosed. Intake of energy, macronutrient (protein, fat, carbohydrate), micronutrient (iron, vitamin C, Copper, Zinc, vitamin B12), inhibitor factors (tannin, oxalate, phytate, fiber), also intake of fruit, vegetable, coffee, and tea, collected using semi quantitative food frequency questionnaire (SQFFQ), 3 months before and 3 months after iron deficiency anemia diagnosis. Data calculated with Nutrisurvey® and STATA 12® for paired T-test. Result: No significant changes (p>0,05) in energy, protein, fat, zinc, vitamin B12, copper, fiber, tannin, and oxalate intake. There were significant improvement in intake of carbohydrate (p=0,0161), iron (p=0,0057), phytate (p=0,000), and vitamin C (p=0,0017). No significant changes in mean intake of fruit, vegetable, and tea servings (p>0,05), but higher consumption of coffee (p=0,0018). Conclusion: Iron deficiency anemia diagnosis resulted in dietary intake changes. Subjects were more aware of their dietary intake after iron deficiency anemia diagnosis. Small fruit and vegetable intake and high tea and coffee consumption suggested that efforts were needed to encourage dietary changes in these foods. (Health Science Journal of Indonesia 2020;11(1):27-31)
{"title":"Dietary intake changes in adolescent girl after iron deficiency anemia diagnosis","authors":"D. Atmaka, Windi Indah Fajar Ningsih, Risnukathulistiwi Maghribi","doi":"10.22435/hsji.v11i1.3143","DOIUrl":"https://doi.org/10.22435/hsji.v11i1.3143","url":null,"abstract":"Background: Iron deficiency may happen because of low consumption of foods rich in bioavailable iron and high consumption of foods rich in inhibitors of iron absorption. Improving dietary intake is the most effective way to break the chain of iron deficiency anemia problems. This study aimed to study the changes in dietary intake and health awareness among adolescent girl before and after iron deficiency anemia diagnosis. Method: Cohort study with 62 subjects from 2 junior high school in Wates after hemoglobin test (cyanmethemoglobin method) and were newly iron deficiency anemia diagnosed. Intake of energy, macronutrient (protein, fat, carbohydrate), micronutrient (iron, vitamin C, Copper, Zinc, vitamin B12), inhibitor factors (tannin, oxalate, phytate, fiber), also intake of fruit, vegetable, coffee, and tea, collected using semi quantitative food frequency questionnaire (SQFFQ), 3 months before and 3 months after iron deficiency anemia diagnosis. Data calculated with Nutrisurvey® and STATA 12® for paired T-test. Result: No significant changes (p>0,05) in energy, protein, fat, zinc, vitamin B12, copper, fiber, tannin, and oxalate intake. There were significant improvement in intake of carbohydrate (p=0,0161), iron (p=0,0057), phytate (p=0,000), and vitamin C (p=0,0017). No significant changes in mean intake of fruit, vegetable, and tea servings (p>0,05), but higher consumption of coffee (p=0,0018). Conclusion: Iron deficiency anemia diagnosis resulted in dietary intake changes. Subjects were more aware of their dietary intake after iron deficiency anemia diagnosis. Small fruit and vegetable intake and high tea and coffee consumption suggested that efforts were needed to encourage dietary changes in these foods. (Health Science Journal of Indonesia 2020;11(1):27-31)","PeriodicalId":30666,"journal":{"name":"Health Science Journal of Indonesia","volume":"11 1","pages":"27-31"},"PeriodicalIF":0.0,"publicationDate":"2020-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45193800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-06-29DOI: 10.22435/hsji.v11i1.3066
D. Rahayu, D. Indarto
Background: Iron deficiency anemia (IDA) is a nutritional problem that occurs in female adolescents around the world. Iron absorption in the small intestine is influenced by the presence of vitamin C, calcium, and zinc in ingested foods. This study aimed to investigate the relationship of vitamin A, vitamin C, calcium, and zinc intake with IDA in female adolescents. Methods: This case-control study was conducted in 60 anemic and 58 normal female adolescents. IDA diagnosis was determined using Hb levels and erythrocyte indexes and the semiquantitative food frequency questionnaire was used to determine vitamin and mineral intake. All collected data were analyzed using chisquare and multiple logistic regression tests with p<0.05. Results: All groups had an adequate intake of vitamin A and C but they had inadequate intake of calcium and zinc (in mg). However, they all had different frequencies in consuming those micronutrients. Rare intake of vitamin A (OR=2.67; CI95%=1.10-6.50; p=0.03) and frequent intake of calcium (OR=2.27; CI95%=0.856.03; p=0.10) increased IDA, compared with frequent intake of vitamin A and rare intake of calcium but only vitamin A intake had a significant effect. Conclusion: Our findings suggest that a higher risk of IDA in female adolescents is related to a rare intake of vitamin A. (Health Science Journal of Indonesia 2020;11(1):52-6)
{"title":"A case-control study related to vitamin and mineral intake in female adolescents with iron deficiency anemia","authors":"D. Rahayu, D. Indarto","doi":"10.22435/hsji.v11i1.3066","DOIUrl":"https://doi.org/10.22435/hsji.v11i1.3066","url":null,"abstract":"Background: Iron deficiency anemia (IDA) is a nutritional problem that occurs in female adolescents around the world. Iron absorption in the small intestine is influenced by the presence of vitamin C, calcium, and zinc in ingested foods. This study aimed to investigate the relationship of vitamin A, vitamin C, calcium, and zinc intake with IDA in female adolescents. Methods: This case-control study was conducted in 60 anemic and 58 normal female adolescents. IDA diagnosis was determined using Hb levels and erythrocyte indexes and the semiquantitative food frequency questionnaire was used to determine vitamin and mineral intake. All collected data were analyzed using chisquare and multiple logistic regression tests with p<0.05. Results: All groups had an adequate intake of vitamin A and C but they had inadequate intake of calcium and zinc (in mg). However, they all had different frequencies in consuming those micronutrients. Rare intake of vitamin A (OR=2.67; CI95%=1.10-6.50; p=0.03) and frequent intake of calcium (OR=2.27; CI95%=0.856.03; p=0.10) increased IDA, compared with frequent intake of vitamin A and rare intake of calcium but only vitamin A intake had a significant effect. Conclusion: Our findings suggest that a higher risk of IDA in female adolescents is related to a rare intake of vitamin A. (Health Science Journal of Indonesia 2020;11(1):52-6)","PeriodicalId":30666,"journal":{"name":"Health Science Journal of Indonesia","volume":"11 1","pages":"52-56"},"PeriodicalIF":0.0,"publicationDate":"2020-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43336319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-06-29DOI: 10.22435/hsji.v11i1.3070
F. Makkiyah, S. Nobel, Rahma Hida Nurrizka
Background: Intraventricular haemorrhage (IVH) worsen the prognosis of Intracerebral hematoma (ICH). External Ventricular Drain (EVD) is inserted to reduce intracranial pressure that resulted from the progression of IVH. However, EVD is still an optional procedure because it is not always proven effective. This study was aimed to demonstrate prognostic factors of IVH and whether EVD insertion might improve the outcome. Methods: This cross-sectional retrospective study included IVH patients and concomitant ICH-IVH that required or not EVD in Cileungsi Hospital from January to December 2018. We made comparisons between EVD insertion group and non-EVD group based on sex, age, pulse pressure, Glasgow Coma Scale (GCS), bleeding volume, score of bleeding volume in ventricle lateral, third and fourth based on CT scan, Charlson Comorbid Index (CCI) and modified Rankin Scale (mRS). To determine factors contributing to the good prognosis of EVD, Spearman Correlation test was used with STATA 15 software. Results: 100 patients were diagnosed with ICH, five patients IVH, 16 patients ICH and IVH. Blood in the fourth ventricle made a significant difference between EVD and non-EVD groups in the concomitant ICHIVH group (p=0.035). GCS score (p=0.034) correlated significantly with the prognosis of concomitant ICHIVH patients that had EVD insertion with correlation coefficient 0.671. EVD did not improve the outcome in IVH patients nor patients with ICH-IVH. Conclusion: EVD did not improve the prognosis of spontaneous concomitant ICH-IVH or spontaneous IVH patients, but it still needs to be inserted in case of developing obstructive hydrocephalus that might be lethal. (Health Science Journal of Indonesia 2020;11(1):1-8)
{"title":"Role of external ventricular drainage in spontaneous intraventricular haemorrhage patients in cileungsi district hospital","authors":"F. Makkiyah, S. Nobel, Rahma Hida Nurrizka","doi":"10.22435/hsji.v11i1.3070","DOIUrl":"https://doi.org/10.22435/hsji.v11i1.3070","url":null,"abstract":"Background: Intraventricular haemorrhage (IVH) worsen the prognosis of Intracerebral hematoma (ICH). External Ventricular Drain (EVD) is inserted to reduce intracranial pressure that resulted from the progression of IVH. However, EVD is still an optional procedure because it is not always proven effective. This study was aimed to demonstrate prognostic factors of IVH and whether EVD insertion might improve the outcome. Methods: This cross-sectional retrospective study included IVH patients and concomitant ICH-IVH that required or not EVD in Cileungsi Hospital from January to December 2018. We made comparisons between EVD insertion group and non-EVD group based on sex, age, pulse pressure, Glasgow Coma Scale (GCS), bleeding volume, score of bleeding volume in ventricle lateral, third and fourth based on CT scan, Charlson Comorbid Index (CCI) and modified Rankin Scale (mRS). To determine factors contributing to the good prognosis of EVD, Spearman Correlation test was used with STATA 15 software. Results: 100 patients were diagnosed with ICH, five patients IVH, 16 patients ICH and IVH. Blood in the fourth ventricle made a significant difference between EVD and non-EVD groups in the concomitant ICHIVH group (p=0.035). GCS score (p=0.034) correlated significantly with the prognosis of concomitant ICHIVH patients that had EVD insertion with correlation coefficient 0.671. EVD did not improve the outcome in IVH patients nor patients with ICH-IVH. Conclusion: EVD did not improve the prognosis of spontaneous concomitant ICH-IVH or spontaneous IVH patients, but it still needs to be inserted in case of developing obstructive hydrocephalus that might be lethal. (Health Science Journal of Indonesia 2020;11(1):1-8)","PeriodicalId":30666,"journal":{"name":"Health Science Journal of Indonesia","volume":"11 1","pages":"1-8"},"PeriodicalIF":0.0,"publicationDate":"2020-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47363618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-30DOI: 10.22435/hsji.v12i2.2239
N. Hardiany, S. Sucitra, R. Paramita
Background: Malondialdehyde (MDA) is a marker of oxidative stress as an end product from the chain reaction of lipid peroxidation. In order to prevent oxidative stress, our body synthesizes catalase, an endogenous antioxidant enzyme that catalyzes hydrogen peroxide (H2O2) into water and oxygen. Until now, the level of MDA and catalase in aging population were still varied and those level at different age in elderly population has not been yet reported. Therefore, the purpose of this study was to analyse the profile of MDA level and catalase specific activity in plasma of elderly women based on increasing age. Methods: This research used 60 healthy elderly women as the subjects living in Jakarta. The subjects were divided into 2 groups based on age category, the younger group (60 – 70 years old) and the older group (>70 years old). MDA and specific activity of catalase were analyzed in plasma using spectrophotometer. Results: MDA level in the younger group (60-70 years old) was slightly higher than MDA levels in the older group (>70 years old) but it was not significant. Moreover, specific activity of catalase in the younger group was significantly lower than the older group. Conclusions: There was no difference in MDA level of elderly woman between younger and older group. However, catalase specific activity significantly increased with increasing age. (Health Science Journal of Indonesia 2019;10(2):132-6)
{"title":"Profile of malondialdehyde (MDA) and catalase specific activity in plasma of elderly woman","authors":"N. Hardiany, S. Sucitra, R. Paramita","doi":"10.22435/hsji.v12i2.2239","DOIUrl":"https://doi.org/10.22435/hsji.v12i2.2239","url":null,"abstract":"Background: Malondialdehyde (MDA) is a marker of oxidative stress as an end product from the chain reaction of lipid peroxidation. In order to prevent oxidative stress, our body synthesizes catalase, an endogenous antioxidant enzyme that catalyzes hydrogen peroxide (H2O2) into water and oxygen. Until now, the level of MDA and catalase in aging population were still varied and those level at different age in elderly population has not been yet reported. Therefore, the purpose of this study was to analyse the profile of MDA level and catalase specific activity in plasma of elderly women based on increasing age. Methods: This research used 60 healthy elderly women as the subjects living in Jakarta. The subjects were divided into 2 groups based on age category, the younger group (60 – 70 years old) and the older group (>70 years old). MDA and specific activity of catalase were analyzed in plasma using spectrophotometer. Results: MDA level in the younger group (60-70 years old) was slightly higher than MDA levels in the older group (>70 years old) but it was not significant. Moreover, specific activity of catalase in the younger group was significantly lower than the older group. Conclusions: There was no difference in MDA level of elderly woman between younger and older group. However, catalase specific activity significantly increased with increasing age. (Health Science Journal of Indonesia 2019;10(2):132-6)","PeriodicalId":30666,"journal":{"name":"Health Science Journal of Indonesia","volume":"10 1","pages":"132-136"},"PeriodicalIF":0.0,"publicationDate":"2020-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46147786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-12-31DOI: 10.22435/hsji.v12i2.2449
S. Dewi, M. Sadikin, M. Ramli, S. Wanandi
Background: Breast cancer stem cells (BCSCs) are subpopulation of cancer cells that has the ability to generate new tumor and similar properties to stem cell. Our previous study using breast cancer patients revealed that gene expression of histone deacetylase 2 (HDAC2) and proliferating cell nuclear antigen (PCNA) were significantly altered after neoadjuvant hormone and chemotherapy. This study aimed to analyze the correlation between HDAC2 and PCNA expressions with the viability of breast cancer stem cells aldehyde dehydrogenase + (BCSC ALDH+) treated by endoxifen. Method: Samples are human primary BCSCs ALDH+ that treated with 4 uM of endoxifen for 2, 4, 6, 8, 10, 12, 14 days, respectively. Cell viability was measured using trypan blue exclusion assay and the mRNA expressions of HDAC2 and PCNA were determined using qRT-PCR. Results: The viability of BCSCs ALDH+ was decreased after 2 days until 4 days-endoxifen treatment. It also demonstrated that mRNA expression of HDAC2 and PCNA were decreased in this period. But after 8 daysendoxifen treatment, the viability of BCSCs ALDH+ was increased. The increasing of viability was higher in 14 days-endoxifen treatment. The mRNA expression of HDAC2 and PCNA also showed increasing begin on 8 days and continued to increase until 14-days endoxifen treatment. We found a similar pattern between HDAC2 and PCNA expression and cell viability Conclusion: Prolonge endoxifen treatment decrease sensitivity of endoxifen effect in human BCSC and the expression of HDAC2 and PCNA are correlated to human BCSCs viability after endoxifen treatment. (Health Science Journal of Indonesia 2019;10(2):77-81)
{"title":"HDAC2 and PCNA expression is correlated to decreasing of endoxifen sensitivity in human breast cancer stem cells ALDH+","authors":"S. Dewi, M. Sadikin, M. Ramli, S. Wanandi","doi":"10.22435/hsji.v12i2.2449","DOIUrl":"https://doi.org/10.22435/hsji.v12i2.2449","url":null,"abstract":"Background: Breast cancer stem cells (BCSCs) are subpopulation of cancer cells that has the ability to generate new tumor and similar properties to stem cell. Our previous study using breast cancer patients revealed that gene expression of histone deacetylase 2 (HDAC2) and proliferating cell nuclear antigen (PCNA) were significantly altered after neoadjuvant hormone and chemotherapy. This study aimed to analyze the correlation between HDAC2 and PCNA expressions with the viability of breast cancer stem cells aldehyde dehydrogenase + (BCSC ALDH+) treated by endoxifen. Method: Samples are human primary BCSCs ALDH+ that treated with 4 uM of endoxifen for 2, 4, 6, 8, 10, 12, 14 days, respectively. Cell viability was measured using trypan blue exclusion assay and the mRNA expressions of HDAC2 and PCNA were determined using qRT-PCR. Results: The viability of BCSCs ALDH+ was decreased after 2 days until 4 days-endoxifen treatment. It also demonstrated that mRNA expression of HDAC2 and PCNA were decreased in this period. But after 8 daysendoxifen treatment, the viability of BCSCs ALDH+ was increased. The increasing of viability was higher in 14 days-endoxifen treatment. The mRNA expression of HDAC2 and PCNA also showed increasing begin on 8 days and continued to increase until 14-days endoxifen treatment. We found a similar pattern between HDAC2 and PCNA expression and cell viability Conclusion: Prolonge endoxifen treatment decrease sensitivity of endoxifen effect in human BCSC and the expression of HDAC2 and PCNA are correlated to human BCSCs viability after endoxifen treatment. (Health Science Journal of Indonesia 2019;10(2):77-81)","PeriodicalId":30666,"journal":{"name":"Health Science Journal of Indonesia","volume":"10 1","pages":"77-81"},"PeriodicalIF":0.0,"publicationDate":"2019-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41528098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background:The treatment of HIV infected children is a challenge to their caregiver due to many existing problems related to their health. Methods: A research to explore the experience and social support on the treatment of HIV infected children was conducted in 5 provinces in Indonesia with highest prevalence of HIV. Total children sample was 239 out of previous 267 planned. Data was collected through semi structured interviews with caregivers of the children. The analysis was conducted to 165 children aged 1-14 years old who were on antiretroviral therapy. Results: Among those 165 children, 63.6% took 1-2 items of medicines and 36.4% took 3-5 items. The most frequent adverse events were skin rash followed by nausea and vomiting. Boredom and questioning were the most frequent difficulties experienced by children aged 5-14 years old. The caregivers attempted to continue the treatment by reminding the children on schedule to take medicines, wheedling, explaining, forcing or even threatening them. The difficulties appeared more as the children grew older. The most frequent supports mainly came from parents, and extended family such as grandmother or uncle especially for orphaned children. Conclusion: Understanding obstacles in HIV infected children will help to do proper interventions to improve adherence that will lead to successful therapy. (Health Science Journal of Indonesia 2019;10(2):103-10)
{"title":"Challenges and social support provisions in the treatment of HIV infected children in Indonesia","authors":"Y. Yuniar, R. Handayani","doi":"10.22435/hsji.v10i2.684","DOIUrl":"https://doi.org/10.22435/hsji.v10i2.684","url":null,"abstract":"Background:The treatment of HIV infected children is a challenge to their caregiver due to many existing problems related to their health. Methods: A research to explore the experience and social support on the treatment of HIV infected children was conducted in 5 provinces in Indonesia with highest prevalence of HIV. Total children sample was 239 out of previous 267 planned. Data was collected through semi structured interviews with caregivers of the children. The analysis was conducted to 165 children aged 1-14 years old who were on antiretroviral therapy. Results: Among those 165 children, 63.6% took 1-2 items of medicines and 36.4% took 3-5 items. The most frequent adverse events were skin rash followed by nausea and vomiting. Boredom and questioning were the most frequent difficulties experienced by children aged 5-14 years old. The caregivers attempted to continue the treatment by reminding the children on schedule to take medicines, wheedling, explaining, forcing or even threatening them. The difficulties appeared more as the children grew older. The most frequent supports mainly came from parents, and extended family such as grandmother or uncle especially for orphaned children. Conclusion: Understanding obstacles in HIV infected children will help to do proper interventions to improve adherence that will lead to successful therapy. (Health Science Journal of Indonesia 2019;10(2):103-10)","PeriodicalId":30666,"journal":{"name":"Health Science Journal of Indonesia","volume":"10 1","pages":"103-110"},"PeriodicalIF":0.0,"publicationDate":"2019-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45375711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-12-31DOI: 10.22435/hsji.v12i2.2441
M. Abdullah, Debby Desmarini, Sofy Meilaini, P. Sari, L. Yunaini, Fadilah Fadilah
Introduction: Colorectal cancer is a pathological transformation of normal colon and rectum epithelial that becomes an abnormal tissue mass, due to the overexpression of cyclin D1 protein that inducing excessive proliferation of colorectal cell. The treatment and prevention of colorectal cancer could be done naturally by consuming leaves extract of Annona muricata L. (soursop). Soursop is known for many phytochemical components that serve as an anti-cancer. Methods: This study was used HT-29 colorectal cancer cell that treated with ethanolic leaves extract of soursop and 5-Fluorourasil (5-FU) to find the cytotoxicity concentration that can inhibit 50% of HT-29 cell population (CC50) and the next concentrations of them were treated for next treatment with MTT assay. Molecular docking analysis of the compounds of ethanolic leaves extract of soursop to cyclin D1 protein used molecular operating environment (MOE) 2013.08 software. Results: CC50 of ethanolic leaves extracts of soursop was 278 μg/mL dan 5-FU was 88 μg/mL. The lowest percentage of viable HT-29 cell was 2 x CC50 after ethanolic leaves extract of soursop treatment (40,4±1,3%) was compared to 5-FU (52,8±4,3%), solvent control (97,2±1,4%), and cells control (100%). Analysis of molecular docking to cyclin D1 protein was obtained N-hexadecanoic acid and phytol molecules as good candidates to inhibit cyclin D1 protein. Conclusions: The ethanolic leaves extract of soursop could be a good alternative treatment for colorectal cancer and its compounds had ability to inhibit cyclin D1 protein (the highest gibbs free energy (ΔG) and affinity (pKi)). (Health Science Journal of Indonesia 2019;10(2):96-102)
{"title":"The effect of ethanolic leaves extract of soursop (Annona muricata L.) on human colorectal cancer cell line: cell viability and in silico study to cyclin D1 protein","authors":"M. Abdullah, Debby Desmarini, Sofy Meilaini, P. Sari, L. Yunaini, Fadilah Fadilah","doi":"10.22435/hsji.v12i2.2441","DOIUrl":"https://doi.org/10.22435/hsji.v12i2.2441","url":null,"abstract":"Introduction: Colorectal cancer is a pathological transformation of normal colon and rectum epithelial that becomes an abnormal tissue mass, due to the overexpression of cyclin D1 protein that inducing excessive proliferation of colorectal cell. The treatment and prevention of colorectal cancer could be done naturally by consuming leaves extract of Annona muricata L. (soursop). Soursop is known for many phytochemical components that serve as an anti-cancer. Methods: This study was used HT-29 colorectal cancer cell that treated with ethanolic leaves extract of soursop and 5-Fluorourasil (5-FU) to find the cytotoxicity concentration that can inhibit 50% of HT-29 cell population (CC50) and the next concentrations of them were treated for next treatment with MTT assay. Molecular docking analysis of the compounds of ethanolic leaves extract of soursop to cyclin D1 protein used molecular operating environment (MOE) 2013.08 software. Results: CC50 of ethanolic leaves extracts of soursop was 278 μg/mL dan 5-FU was 88 μg/mL. The lowest percentage of viable HT-29 cell was 2 x CC50 after ethanolic leaves extract of soursop treatment (40,4±1,3%) was compared to 5-FU (52,8±4,3%), solvent control (97,2±1,4%), and cells control (100%). Analysis of molecular docking to cyclin D1 protein was obtained N-hexadecanoic acid and phytol molecules as good candidates to inhibit cyclin D1 protein. Conclusions: The ethanolic leaves extract of soursop could be a good alternative treatment for colorectal cancer and its compounds had ability to inhibit cyclin D1 protein (the highest gibbs free energy (ΔG) and affinity (pKi)). (Health Science Journal of Indonesia 2019;10(2):96-102)","PeriodicalId":30666,"journal":{"name":"Health Science Journal of Indonesia","volume":"10 1","pages":"96-102"},"PeriodicalIF":0.0,"publicationDate":"2019-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45201314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-12-30DOI: 10.22435/hsji.v10i2.2444
Serawati Dewi, Yuni Romalita, Yusriani Yusriani, M. Alwi
Background: The Maternal Mortality Rate (MMR) is one indicator of health development in Indonesia. Efforts to accelerate the reduction of MMR can be done by ensuring that every mother can access quality maternal health services. Antenatal care is utilized properly, maternal health can be monitored continuously from pregnancy to delivery. Methods: The study design was observational with a cross sectional study design. The population in this study were all pregnant women who had a gestational age of 7-9 months in Gowa Regency as many as 122 people. A sample of 93 people was taken using accidental sampling technique. Results: Pregnant women who have a perception of small monetary sacrifice and say the role of the health workers is lacking in antenatal care 90.0%. Pregnant women who have a perception of monetary sacrifice are very small and say the role of health workers is less as much as 83.1%. Pregnant women who have the perception of the sacrifice of big time and say the role of health workers lacking in antenatal care as much as 100%. Pregnant women who have a perception great monetary sacrifice and say the role of health workers is lacking in antenatal care 90.2%. Conclusion: Satisfaction of pregnant women towards the role health workers in antenatal care based on perception monetary sacrifice does not show correlation while based on time sacrifice shows there is a correlation. Need to increase the speed of the process of pregnancy examination services for pregnant women by health workers. (Health Science Journal of Indonesia 2019;10(2):111-8)
{"title":"Perceptions of pregnant woman on monetary and time sacrifice for satisfaction based on health workers roles in antenatal services to reduce the risk of maternal death at Gowa district","authors":"Serawati Dewi, Yuni Romalita, Yusriani Yusriani, M. Alwi","doi":"10.22435/hsji.v10i2.2444","DOIUrl":"https://doi.org/10.22435/hsji.v10i2.2444","url":null,"abstract":"Background: The Maternal Mortality Rate (MMR) is one indicator of health development in Indonesia. Efforts to accelerate the reduction of MMR can be done by ensuring that every mother can access quality maternal health services. Antenatal care is utilized properly, maternal health can be monitored continuously from pregnancy to delivery. Methods: The study design was observational with a cross sectional study design. The population in this study were all pregnant women who had a gestational age of 7-9 months in Gowa Regency as many as 122 people. A sample of 93 people was taken using accidental sampling technique. Results: Pregnant women who have a perception of small monetary sacrifice and say the role of the health workers is lacking in antenatal care 90.0%. Pregnant women who have a perception of monetary sacrifice are very small and say the role of health workers is less as much as 83.1%. Pregnant women who have the perception of the sacrifice of big time and say the role of health workers lacking in antenatal care as much as 100%. Pregnant women who have a perception great monetary sacrifice and say the role of health workers is lacking in antenatal care 90.2%. Conclusion: Satisfaction of pregnant women towards the role health workers in antenatal care based on perception monetary sacrifice does not show correlation while based on time sacrifice shows there is a correlation. Need to increase the speed of the process of pregnancy examination services for pregnant women by health workers. (Health Science Journal of Indonesia 2019;10(2):111-8)","PeriodicalId":30666,"journal":{"name":"Health Science Journal of Indonesia","volume":"10 1","pages":"111-118"},"PeriodicalIF":0.0,"publicationDate":"2019-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43105221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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