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Measurement of Plant Stomas under Several CO_2 Conditions using Microscopic Images 几种CO_2条件下植物气孔的显微图像测量
Pub Date : 2004-06-01 DOI: 10.2525/JSHITA.16.41
K. Namba, N. Kondo, M. Monta, A. Sasao
The objective of this study was to create a model of the plant stoma response process under CO2 conditions (100 to 3000ppm) after lighting. In this study, a real-time measurement system was developed to observe living plant stomas continuously for a long period in a growth chamber using a microscope and a color TV camera. Six treatments were conducted by changing the CO2 density, ranging from 100 to 3000ppm, under the conditions of a 2-hour on/off light cycle. From the experimental results, it was observed that the pore size was drastically changed in response to the CO2 conditions. Lower CO2 density caused larger-size pores under conditions of less than 1000ppm CO2 density, while the pore size was almost constant under conditions of greater density. With CO2 density of over 2000ppm, pores continued opening after 60 minutes had elapsed. The transient response with the 1 st-order lag element could well express the stoma response, and its desired value had a high correlation with CO2 density.
本研究的目的是建立光照后CO2 (100 ~ 3000ppm)条件下植物气孔响应过程的模型。本研究开发了一种实时测量系统,利用显微镜和彩色电视摄像机在生长室内长时间连续观察活植物气孔。在2小时的光照循环条件下,通过改变CO2浓度(从100到3000ppm)进行了6次处理。从实验结果中可以观察到,在CO2条件下,孔隙大小发生了巨大的变化。当CO2浓度低于1000ppm时,较低的CO2密度使孔隙尺寸变大,而在较高的CO2密度下,孔隙尺寸基本保持不变。当二氧化碳浓度超过2000ppm时,孔隙在60分钟后继续打开。1阶滞后元的瞬态响应能很好地表达气孔响应,其期望值与CO2浓度有较高的相关性。
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引用次数: 1
Micropropagation of Glaucidium palmatum Sieb. et Zucc. by Somatic Embryogenesis from Immature Seeds and Cytogenetic Stability of Culture 掌叶菖蒲的微繁研究。调查。通过未成熟种子的体细胞胚胎发生和培养的细胞遗传稳定性
Pub Date : 2003-12-01 DOI: 10.2525/JSHITA.15.191
K. Ishii, H. Sasaki, H. Ueno
Somatic embryos were initiated from immature seeds of Glaucidium palmatum Sieb. et Zucc. (which is indigenous only in Japan and bears beautiful flowers, however recently extinction by deer attacks is problematic) on the half strength Murashige and Skoog medium containing 2.22μM 6-benzvlaminoDurine. Somatic embrvos can be propagated on the same medium. Temperature of the incubation chamber was one of the important factors for better propagation and growth of the somatic embryos. Plant regeneration was successful on the plant growth regulator free medium. Regenerated plantlets were cytogenetically stable according to the flow cytometric study and DNA content was 1. 35 pg/cell. They were habituated and planted out in the field. Tissue culture can overcome the extinction of potentially endangered species such as Glaucidium palmatum.
以掌叶蓝(Glaucidium palmatum Sieb)的未成熟种子为材料,进行了体细胞胚的初始化。调查。(它只生长在日本,开着美丽的花,但最近因鹿的袭击而灭绝是有问题的)在含有2.22μM 6-苄基氨基二磺酸的半强度Murashige和Skoog培养基上。体细胞胚可以在同一培养基上繁殖。培养室温度是影响体胚繁殖和生长的重要因素之一。植株在无生长调节剂培养基上再生成功。流式细胞术显示再生植株细胞遗传学稳定,DNA含量为1。35 pg /单元。它们被种植在地里。组织培养可以克服潜在濒危物种的灭绝,如掌兰。
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引用次数: 0
Sweetpotato Propagule Production Rate and Electric Energy Consumption in a Closed Transplant Production System as Affected by Planting Density 种植密度对封闭式移栽系统甘薯繁殖率和电能消耗的影响
Pub Date : 2002-03-01 DOI: 10.2525/JSHITA.14.10
L. Hin, K. Ohyama, C. Kubota, T. Kozai
Multiplication ratio (number of average of harvestable propagules per stock plant, M) and electric energy consumption per propagule (Ep) at four levels of planting density (59, 118, 236 and 473 m-2) were investigated for sweetpotato (Ipomoea batatas (L.) Lam.) vegetative propagation in a closed transplant production system. After n multiplication cycles, total number of harvestable propagules (Nn) and electric energy consumption per propagule (Ep, n) in the system were estimated by using mathematical models. Single nodal cuttings were used as propagules and grown to 14 days under photosynthetic photon flux of 140, 200 and 320μ mol·m-2·s-1 during 1 to 5, 6 to 10 and 11 to 14 days after planting, respectively. Other environmental conditions included photoperiod of 16 h·d-1, air temperature of 29°C, and CO2 concentration of 920μmol·mo1-1. At the planting densities of 59, 118, 236 and 473 m-2, M were 5.9, 6.4, 3.8 and 2.3, respectively, and Ep, 1 were 0.41, 0.19, 0.16 and 0.13 kWh (electricity cost : 6.2, 2.9, 2.4 and 2.0 Yen), respectively. Results suggest that production can be optimized and electricity cost can be reduced by manipulating planting densities.
研究了4种种植密度(59、118、236和473 M -2)下甘薯(Ipomoea batatas, L.)的增殖比(平均每株可收获繁殖体数,M)和每繁殖体电能消耗(Ep)。在封闭的移栽生产系统中无性繁殖。经过n个繁殖周期后,利用数学模型估算出系统中可收获的繁殖体总数(Nn)和每个繁殖体的电能消耗(Ep, n)。以单节扦插作为繁殖体,分别于植后1 ~ 5、6 ~ 10和11 ~ 14 d,在140、200和320μ mol·m-2·s-1的光合光子通量下生长至14 d。其他环境条件为光周期16 h·d-1,空气温度29℃,CO2浓度920μmol·mo1-1。在种植密度为59、118、236和473 M -2时,M分别为5.9、6.4、3.8和2.3,Ep、1分别为0.41、0.19、0.16和0.13 kWh(电费分别为6.2、2.9、2.4和2.0日元)。结果表明,通过控制种植密度可以优化生产,降低电力成本。
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引用次数: 11
Tomographic Imaging of Development of Mycelium Inside of Medium for Grifora Frondosa using MRI System 灰树花培养基内菌丝体发育的MRI层析成像
Pub Date : 2001-09-01 DOI: 10.2525/JSHITA.13.167
J. Hirama, T. Miyamoto, Ryou Ishida, Ryouichi Suzuki, Kouzou Nishibori, Y. Ohdaira
MRIの断層撮影によって, 舞茸の培地内部における菌糸の発達状態を非破壊的に観測できることがわかった, 今後は, 複数の別個体や植菌直後から原基形成までの期間の状態などでも断層撮影を活用し, 菌糸の状態を系統的に観測するとともに, 舞茸の地上部である子実体の成長状態と菌床内部の菌糸の発達状態との関係についても検討を進める予定である.なお, 本研究の成果の一部は, 文部省科学研究費 (基盤研究 (C) : 課題番号12650435) の助成による.
通过MRI断层扫描,可以非破坏性地观测舞菇培养基内部菌丝的发育状态。今后,还可以对多个不同个体以及植菌之后到原基形成期间的状态等进行断层扫描,在系统地观测菌丝状态的同时,还将对作为舞茸地上部分的子实体的生长状态与菌床内部菌丝的发育状态之间的关系进行探讨。根据文部省科学研究费(基础研究(C):课题号12650435)的资助。
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引用次数: 2
Development for measuring method for cuticle resistance of plant shoot tip. 植物茎尖角质层阻力测量方法的发展。
Pub Date : 2000-09-01 DOI: 10.2525/JSHITA.12.155
H. Shimizu, R. Heins
1. 茎頂部のエネルギーバランス式と実験的に容易に求めることができるdVPD/d (Tair-Tp) (飽差の変化率と, 乾球温度と茎頂部温度との差の変化率の比) を組み合わせることによって茎頂部のクチクラ抵抗を求める式を導出した.2. 本研究で提案した方法でポインセチア茎頂部のクチクラ抵抗を測定したところ, 約960 (s/m) となった.茎頂部のクチクラ抵抗を測定した報告が見当たらないので, この値の妥当性を定量的に評価することは難しいが, トマトやナスなどの葉のクチクラ抵抗は約1200 (s/m) との報告がある.3. 一般に茎頂部のクチクラ抵抗は種や生長ステージによって異なると考えられるので, 必要に応じて容易に測定することが可能な方法が求められるが, 本研究で提案している方法はこの条件を満たしており実用的であると考える.
茎顶部能量平衡式和实验上容易求出的dVPD/d (Tair-Tp)(饱差变化率和,结合干球温度与茎顶温度之差变化率之比),得出了求得茎顶口阻力的公式。用本研究中提出的方法测量了一品红茎顶部的口腹阻力,结果约为960 (s/m)。由于没有找到测定茎顶部的唇阻力的报告,因此很难定量评价该值的有效性,但有报告称西红柿、茄子等叶子的唇阻力约为1200 (s/m)。一般认为茎顶部的喙抵抗根据种子和生长阶段的不同而不同,所以要求根据需要容易测定的方法,我们认为本研究中提出的方法满足这个条件并且是实用的。
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引用次数: 3
Effect of laser-diode light on growth of Lactuca sativa L. 激光二极管光对芥蓝生长的影响。
Pub Date : 2000-06-01 DOI: 10.2525/JSHITA.12.93
A. Yamazaki, H. Tsuchiya, H. Miyajima, T. Honma, H. Kan
Development of an effective, high-power, low-cost, artificial light source for use in plantgrowing facilities would be very beneficial for plant production. Recently, the laser-diode lamp has been proposed as a new type of light source for plant production. The advantages of the laser-diode lamp over conventional light sources are its high electrical-to-optical power conversion efficiency, low thermal radiation, easy set-up for high power and pulse irradiation, small weight and small volume for mounting, and selectivity for proper wavelength. Because laser light itself differs from the light sources presently used in plant growing, we confirmed the possibility of growing plants under the laser-diode light by growing lettuce (Lactuca sativa L.) plants. Lettuce seedlings with 5-6 leaves were grown under a laser-diode lamp panel with 30 pieces of high-power and high-efficiency AlGaInP laser-diodes. The power of each laser-diode lamp was 500 mW, and the wavelength was 680 nm, which was efficient for photosynthesis. The lettuce plants were able to grow under the laser-diode light. However, the weight of the lettuce plants grown under the laser-diode lamps was light, and their leaves were long and thin compared to those of lettuce plants grown under high-pressure sodium lamps.
开发一种高效、高功率、低成本的人造光源用于植物生长设施,对植物生产是非常有益的。近年来,激光二极管灯已被提出作为一种新型的植物生产光源。与传统光源相比,激光二极管灯的优点是电光功率转换效率高,热辐射低,易于设置高功率和脉冲辐照,安装重量小,体积小,波长选择性好。由于激光本身不同于目前植物生长中使用的光源,我们通过种植莴苣(Lactuca sativa L.)植物,证实了在激光二极管光下种植植物的可能性。在30片高功率、高效率的AlGaInP激光二极管的激光二极管灯面板下,种植5-6片叶片的莴苣幼苗。每个激光二极管灯的功率为500 mW,波长为680 nm,可以进行有效的光合作用。生菜能够在激光二极管光下生长。然而,与高压钠灯下的生菜相比,激光二极管灯下种植的生菜重量轻,叶子又长又薄。
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引用次数: 4
Regeneration Potential of Horseradish Cells Stored in Artificial Seeds with Limited Oxygen Supply 限氧人工种子对辣根细胞再生潜力的影响
Pub Date : 1999-09-01 DOI: 10.2525/JSHITA.11.173
H. Nagatome, K. Baba, M. Taya, S. Tone
The effect of oxygen concentration on the regeneration potential of horseradish cell aggregates was investigated to develop artificial seeds capable of preserving the viability after long-terms torage at room temperature. When the cell aggregates were incubated for 20days at an oxygen concentration of 1.0―2.Omol/m3 in the gas phase, their viability was maintained without the regeneration of adventitious roots. The artificial seeds were prepared by encap-sulating the cell aggregates in alginate gel with various thicknesses of paraffin coat ranging from 0.35×10-3―1.3×10-3m. It was found that the cell aggregates in the artificial seeds maintained their ability to regenerate adventitious roots after storage for 30 to 60 days , although the efnciency of root regeneration decreased with increasing paraffin coat thickness. The dependence of root regeneration on paraffin coat thickness could be explained based on a model considering oxygen permeation through the alginate
研究了氧浓度对辣根细胞聚集体再生潜能的影响,制备了能在室温下长期保存活力的人工种子。将细胞聚集体在1.0-2氧浓度下培养20d。在无不定根再生的情况下,保持其活力。将细胞聚集体包覆在海藻酸盐凝胶中制备人工种子,并用不同厚度的石蜡包覆0.35×10-3-1.3×10-3m。结果表明,人工种子中的细胞聚集体在贮存30 ~ 60天后仍能保持其再生不定根的能力,但随着石蜡皮厚度的增加,其再生能力有所下降。根再生对石蜡包层厚度的依赖性可以用考虑氧通过海藻酸盐渗透的模型来解释
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引用次数: 1
The Recognition of Visible Injury of Plant Disease Using Bandpass Filtered Images 利用带通滤波图像识别植物病害的可见伤害
Pub Date : 1998-09-01 DOI: 10.2525/JSHITA.10.170
Y. Sasaki, T. Okamoto, K. Imou, T. Torii
以上, キュウリに炭そ病菌を接種して人為的に可視害を作り, 自動診断システムを構築するために必要となる可視害の認識について, フィルター画像を用いて以下のことを実験し考察した.1.可視害部の識別に関してその識別しやすい波長を同定するために, (1) ?ƒk, (2) 画像のクラス分離度ηmax, (3) D.I.で評価した反射率で行い, この三つの評価量に共通して650nmのフィルター画像が認識に適していることが判明した.2.外界での診断に向けて環境条件を除去する基本的なフィルター情報融合法を検討した.これにより環境条件を除去して病気による反射率の変化を得る二つの波長情報はλ650とλ400かλ800であることが判明した.またλ800は反射率が高く葉脈の影響がλ400より強いため, 葉を認識しながら接近する際や比較的遠隔から葉脈に対する可視害部の位置を知りたい場合はλ800を選択し, 近接して可視害部を詳細に調べる時は葉脈の影響が小さいλ400が適すると考察した.問題点と今後の課題は,実際に光源変化や対象の傾きをつくり,環境条件が変化しても同じ診断が下せるよう今回の結果を用いて検証することである.対象である葉は風により静止していない上に外界では光源が太陽光となるために,可視害に至った葉の診断部を構築するためには葉の動きや測定距離にも対応した画像処理に改良していくことも必要である.立毛中の植物を外界で診断するためには上記のような天候・時刻に左右されない測定が可能となることがまず必要である.そして実際には環境ストレス,他の病気を原因とするストレスに植物が侵されると予想される.環境ストレスは主に水不足,土壌環境,日照不足に分けられる.水不足については,今回は800nmまでのフィルター画像しか得られなかったが,Fig.10より植物の水吸収帯が1,400nm付近にあることがわかるため,このフィルター画像を用いた含水診断を行えば良いのではないかと考えている.また他のものはそれぞれ土壌診断,天候のデータの使用により診断できると推測する.病害によるストレスに関しては,対象とした植物に対して発生しやすい病害を特定して基礎データを得て各病害の特徴を統計的に求め,Fig.1に示したように識別のしやすいものから診断することで原因の特定が可能になると考える.また室内で得られた病害のデータが自然界で発生する病害とは多少異なることが予想されるが,その点に関しては新たに得られたデータを入力していけば診断規準が変更できるよう自動診断を学習型とすることで対応できるのではないかと考えている.
综上所述,为了给黄瓜接种炭疽菌,人为地制造可见危害,并构建自动诊断系统,对必要的可见危害的认识,利用过滤图像进行了以下实验。关于可视危害的识别,为了识别其容易识别的波长,(1)?ƒk,(2)以图像的类分离度ηmax, (3) d.i.评估的反射率进行,发现这三个评估量共同适合于650nm的滤色器图像的识别。研究了消除环境条件的基本滤光器信息融合方法,从而发现消除环境条件以获得疾病引起的反射率变化的两种波长信息是λ650和λ400或λ800。另外,λ800的反射率高,对叶脉的影响比λ400强,因此在接近树叶时,或者想从较远距离知道可见危害部位对叶脉的位置时,可以选择λ800。我们认为,在近距离详细调查可见危害区域时,适用于叶脉影响较小的λ400。问题点和今后的课题是,实际制造光源变化和对象斜率,利用此次的结果验证即使环境条件变化也能得出相同的诊断。由于作为对象的叶子因风而不能静止,再加上外界的光源是太阳光,因此,为了构建造成可视危害的叶子的诊断部,需要对对应叶子的运动和测定距离的图像处理进行改良。为了在外界对立毛中的植物进行诊断,首先需要能够进行不受上述天气、时间影响的测定。而且实际上预计植物会受到环境压力、其他疾病等压力的侵袭。环境压力主要分为缺水、土壤环境和日照不足。缺水方面,这次只获得了800nm的滤光片图像,从Fig.10可以看出植物的水吸收带在1,400nm附近。因此,笔者认为使用该过滤器图像进行含水诊断就可以了。另外,推测其他的可以分别通过土壤诊断和使用天气数据进行诊断。关于病害引起的压力,确定对象植物容易发生的病害,得到基础数据,统计求得各病害的特征,如Fig.1所示,从容易识别的部位开始诊断,就可以确定原因。另外,室内得到的病害数据可能与自然界发生的病害多少有些不同,关于这一点,可以将自动诊断作为学习型的,只要输入新得到的数据就可以改变诊断标准。我在想是不是可以用蚂蚁来应对。
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引用次数: 1
Effects of Various Kinds of Water on Plant Growth (Part 4) : Effects of Water Treated with Calcium-Mixed Granite on Plant Growth 各种水对植物生长的影响(四):钙混合花岗岩水处理对植物生长的影响
Pub Date : 1998-06-01 DOI: 10.2525/JSHITA.10.119
T. Umeki, K. Ishikawa, K. Tanabe
The purpose of the study is to investigate the effect of water treated with calcium mixed Granite (Tetsuta village in Okayama), which is an igneous rock composed mainly of silica and aluminum oxide, on plant growth. Plants were grown using desalinated water, tap water (Kumamoto city) and spring water (Tetsuta village), all of which were treated with 100 g stone/ 1000 ml. The pH values and electric conductivity (EC) values were significantly increased by treatment of water. The mean number of germination days and germination coefficient for radish seeds increased slightly with the treated water. Seedling growth increased markedly with desalinated water and the tap water treated with rocks for 5 days.
本研究的目的是研究混合钙花岗岩(冈山哲太村)的水处理对植物生长的影响。混合钙花岗岩是一种主要由二氧化硅和氧化铝组成的火成岩。采用淡化水、自来水(熊本市)和泉水(哲太村)种植,均以100 g stone/ 1000 ml处理。水的处理显著提高了pH值和电导率(EC)值。处理过的水使萝卜种子的平均发芽天数和发芽系数略有增加。淡化水和加石自来水处理5 d后,幼苗生长明显增加。
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引用次数: 3
Effect of Carbon Dioxide in an Optimized Design of the Plant Factory. 二氧化碳在植物工厂优化设计中的作用。
Pub Date : 1998-03-01 DOI: 10.2525/JSHITA.10.5
Y. Mizushima
Based on the theory of plant factory, effect of carbon dioxide concentration on the profit is discussed. A proposed functional form of the growth rate has a multivariable dependence on the carbon dioxide concentration and the irradiance. By optimization of the proposed profit function, an improved operation condition can be selected for the maximum gain. The simulation results quantitatively indicates that the power consumption can be economized by increment of the carbon dioxide concentration.
以植物工厂理论为基础,讨论了二氧化碳浓度对利润的影响。提出的生长速率的函数形式有一个多变量依赖于二氧化碳浓度和辐照度。通过对所提出的利润函数进行优化,可以选择一个改进的操作条件以获得最大的收益。仿真结果定量地表明,增加二氧化碳浓度可以节约电力消耗。
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引用次数: 0
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Shokubutsu Kankyo Kogaku
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