S. Indarti, A. Wibowo, S. Subandiyah, Miftahul Ajri
A survey to investigate the stem and bulb plant parasitic nematode at one of garlic area centre, in Temanggung, Central Java, Indonesia was conducted from Januaryto February 2018. Infected plant with specific symptoms, morphological and morphometric characters both of female and male of adult nematodes were used to describe a A1 quarantine plant parasitic nematode Ditylenchus dipsaci. Seven from nine observed locations were postively infected with population in average 2.67 nematodes per 100 g of soil and 2.67–189.33 per bulb. This is the first report of D. dipsaci from Indonesia and consequently further investigations were needed to know their distribution and also to confirm the origin of the nematode. IntisariSurvei keberadaan nematoda parasit batang dan umbi dilakukan pada bulan Januari–Februari 2018 pada satu sentra penangkaran bawang putih di Temanggung, Jawa Tengah, Indonesia. Tanaman terinfeksi dengan gejala serangan yang spesifik, karakter morfologi dan morfometri nematoda betina dan jantan dewasa dipergunakan dalam identifikasi Ditylenchus dipsaci yang merupakan nematoda parasit tanaman yang termasuk OPTK A1 (Organisme Pengganggu Tanaman Karantina A1) di Indonesia. Sebanyak tujuh dari sembilan lokasi yang diamati mengindikasikan positif sebagai daerah sebaran nematoda tersebut dengan populasi rata-rata 2,67 ekor per 100 gram tanah dan 2,67–189,33 nematoda per umbi. Laporan pertama tentang nematoda D. dipsaci ini membawa konsekuensi perlunya penelitian lebih lanjut untuk mengetahui daerah sebaran dan juga konfirmasi dari mana nematoda tersebut berasal.
{"title":"First Record: A Stem and Bulb Plant Parasitic Nematode at Garlic Area Centre Temanggung, Central Java, Indonesia with Species Reference to Ditylenchus dipsaci","authors":"S. Indarti, A. Wibowo, S. Subandiyah, Miftahul Ajri","doi":"10.22146/jpti.35321","DOIUrl":"https://doi.org/10.22146/jpti.35321","url":null,"abstract":"A survey to investigate the stem and bulb plant parasitic nematode at one of garlic area centre, in Temanggung, Central Java, Indonesia was conducted from Januaryto February 2018. Infected plant with specific symptoms, morphological and morphometric characters both of female and male of adult nematodes were used to describe a A1 quarantine plant parasitic nematode Ditylenchus dipsaci. Seven from nine observed locations were postively infected with population in average 2.67 nematodes per 100 g of soil and 2.67–189.33 per bulb. This is the first report of D. dipsaci from Indonesia and consequently further investigations were needed to know their distribution and also to confirm the origin of the nematode. IntisariSurvei keberadaan nematoda parasit batang dan umbi dilakukan pada bulan Januari–Februari 2018 pada satu sentra penangkaran bawang putih di Temanggung, Jawa Tengah, Indonesia. Tanaman terinfeksi dengan gejala serangan yang spesifik, karakter morfologi dan morfometri nematoda betina dan jantan dewasa dipergunakan dalam identifikasi Ditylenchus dipsaci yang merupakan nematoda parasit tanaman yang termasuk OPTK A1 (Organisme Pengganggu Tanaman Karantina A1) di Indonesia. Sebanyak tujuh dari sembilan lokasi yang diamati mengindikasikan positif sebagai daerah sebaran nematoda tersebut dengan populasi rata-rata 2,67 ekor per 100 gram tanah dan 2,67–189,33 nematoda per umbi. Laporan pertama tentang nematoda D. dipsaci ini membawa konsekuensi perlunya penelitian lebih lanjut untuk mengetahui daerah sebaran dan juga konfirmasi dari mana nematoda tersebut berasal.","PeriodicalId":31599,"journal":{"name":"Jurnal Perlindungan Tanaman Indonesia","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47073944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Susanto, W. D. Natawigena, L. T. Puspasari, N. I. N. Atami
Fruit fly (Bactrocera dorsalis Complex) is one of the major pests in horticultural commodities in Indonesia. In this present study, a control method of formulating methyl eugenol and the addition of fruit essences was tested to attract B. dorsalis Complex. The objective was to find out the effect of fruit essences addition in the performance of methyl eugenol in attracting fruit flies and to obtain the most effective fruit essences for attracting male and female fruit flies. The experiment was conducted on mango plantation in Pasirmuncang village, Majalengka, West Java from March 2016 until December 2016. Several synthetic fruit essences which were separately added to methyl eugenol in this experiment were mango, citrus, guava, and starfruit essences. The results showed that the addition of fruit essences on methyl eugenol traps had the same effectiveness or as good as any single methyl eugenol trap. Moreover, the additions of mango and orange essences were not effective enough in attracting female fruit flies, although the number of female fruit flies that were caught were more than the other treatments. IntisariLalat buah Bactrocera dorsalis Kompleks merupakan salah satu hama utama pada komoditas hortikultura. Pada penelitian ini, metode pengendalian dengan memformulasikan metil eugenol dan tambahan esens buah diuji untuk menarik B. dorsalis Kompleks. Tujuannya adalah untuk mengetahui efek penambahan esens buah pada kinerja perangkap metil eugenol dalam menarik lalat buah serta untuk mendapatkan esens buah yang efektif untuk menarik lalat buah betina. Penelitian ini dilakukan di perkebunan mangga di desa Pasirmuncang, Majalengka, Jawa Barat dari bulan Maret 2016 hingga bulan Desember 2016. Beberapa jenis esens buah sintetik yang secara terpisah ditambahkan pada metil eugenol pada percobaan ini adalah mangga, jeruk, jambu biji, dan belimbing. Hasil penelitian menunjukkan bahwa penambahan esens buah pada perangkap metil eugenol memiliki keefektifan yang sama baiknya dengan perangkap metil eugenol secara tunggal. Selain itu, penambahan esens mangga dan esens jeruk belum cukup efektif dalam menarik lalat buah betina meskipun jumlah lalat buah betina yang tertangkap lebih banyak dibandingkan dengan perlakuan lainnya.
果蝇(Bactrocera dorsalis Complex)是印度尼西亚园艺商品中的主要害虫之一。本研究采用配制甲基丁香酚和添加水果香精的控制方法来吸引B.dorsalis复合体。目的是了解添加水果香精对甲基丁香酚引诱果蝇性能的影响,并获得最有效的引诱雄性和雌性果蝇的水果香精。该实验于2016年3月至2016年12月在西爪哇省马贾伦卡市Pasirmmunang村的芒果种植园进行。本实验中,在甲基丁香酚中分别添加了芒果、柑橘、番石榴和海星果等几种合成水果香精。结果表明,在甲基丁香酚诱捕器上添加水果香精具有与任何单一甲基丁香醇诱捕器相同或相同的效果。此外,添加芒果和橙子精华[UNK]对吸引雌性果蝇的效果不够,尽管捕获的雌性果蝇数量比其他处理多。桔小实蝇是园艺商品中的主要火腿之一。本研究采用配制丁香酚和添加水果香精的防治方法,对桔梗复合物进行引诱试验。目的是了解添加水果香精对丁香酚甲基诱捕器引诱果蝇性能的影响,并获得有效的引诱雌性果蝇的水果香精。本研究于2016年3月至2016年12月在Jawa West Majalenka的Pasirmuncang村进行红树林养殖。在这个实验中,一些单独添加到丁香酚甲基中的合成水果精华是芒果、橙子、种子和膀胱。研究表明,在丁香酚甲基诱捕器中添加水果精华与单一丁香酚甲酯诱捕器具有相同的效果。此外,尽管雌性果蝇的数量比其他处理多,但添加锰精和橙精对吸引雌性果蝇的效果不够。
{"title":"Pengaruh Penambahan Beberapa Esens Buah pada Perangkap Metil Eugenol terhadap Ketertarikan Lalat Buah Bactrocera dorsalis Kompleks pada Pertanaman Mangga di Desa Pasirmuncang, Majalengka","authors":"A. Susanto, W. D. Natawigena, L. T. Puspasari, N. I. N. Atami","doi":"10.22146/JPTI.27001","DOIUrl":"https://doi.org/10.22146/JPTI.27001","url":null,"abstract":"Fruit fly (Bactrocera dorsalis Complex) is one of the major pests in horticultural commodities in Indonesia. In this present study, a control method of formulating methyl eugenol and the addition of fruit essences was tested to attract B. dorsalis Complex. The objective was to find out the effect of fruit essences addition in the performance of methyl eugenol in attracting fruit flies and to obtain the most effective fruit essences for attracting male and female fruit flies. The experiment was conducted on mango plantation in Pasirmuncang village, Majalengka, West Java from March 2016 until December 2016. Several synthetic fruit essences which were separately added to methyl eugenol in this experiment were mango, citrus, guava, and starfruit essences. The results showed that the addition of fruit essences on methyl eugenol traps had the same effectiveness or as good as any single methyl eugenol trap. Moreover, the additions of mango and orange essences were not effective enough in attracting female fruit flies, although the number of female fruit flies that were caught were more than the other treatments. IntisariLalat buah Bactrocera dorsalis Kompleks merupakan salah satu hama utama pada komoditas hortikultura. Pada penelitian ini, metode pengendalian dengan memformulasikan metil eugenol dan tambahan esens buah diuji untuk menarik B. dorsalis Kompleks. Tujuannya adalah untuk mengetahui efek penambahan esens buah pada kinerja perangkap metil eugenol dalam menarik lalat buah serta untuk mendapatkan esens buah yang efektif untuk menarik lalat buah betina. Penelitian ini dilakukan di perkebunan mangga di desa Pasirmuncang, Majalengka, Jawa Barat dari bulan Maret 2016 hingga bulan Desember 2016. Beberapa jenis esens buah sintetik yang secara terpisah ditambahkan pada metil eugenol pada percobaan ini adalah mangga, jeruk, jambu biji, dan belimbing. Hasil penelitian menunjukkan bahwa penambahan esens buah pada perangkap metil eugenol memiliki keefektifan yang sama baiknya dengan perangkap metil eugenol secara tunggal. Selain itu, penambahan esens mangga dan esens jeruk belum cukup efektif dalam menarik lalat buah betina meskipun jumlah lalat buah betina yang tertangkap lebih banyak dibandingkan dengan perlakuan lainnya.","PeriodicalId":31599,"journal":{"name":"Jurnal Perlindungan Tanaman Indonesia","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46409316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Iqna Khayatina Rusli, Arif Wibowo, Christanti Sumardiyono
Wilt disease caused by Fusarium oxysporum f. sp. cubense (Foc) is still pose as constraint to banana production all over the world. The objective of this research was to examine the viability and virulence of Foc isolates which had been preserved with lyophilization for 18 years. The experiment used 19 isolates of Foc, which were Pekulo, Sukorejo, Kali Sepanjang, Kalibaru, Purwojoyo, Prembun, Mulyosari, Sidogede, Sidatan, Kemiri, Juwangen, A-17, A-11, PS-5, B4-3-1, Fo2.16.16., PS-10, A-2, and Irja. The results showed that 12 isolates could grow well on PDA medium, namely Pekulo, Sukorejo, Kali Sepanjang, Kalibaru, Purwojoyo, Prembun, Mulyosari, Sidatan, Juwangen, PS-5, B4-3-1, and Fo2.16.16. Meanwhile, seven isolates (Sidogede, Kemiri, A-17, A-11, PS-10, A-2, and Irja) could not grow or develop on PDA medium. The result of pathogenicity test showed that six tested Foc isolates had very high virulences, with disease severity index of about 2.14–2.71. Other three isolates revealed high virulences with disease severity index around 2.01–2.07. Meanwhile, another three less virulent isolates demonstrated disease severity index in the range of 1.39–1.67. Intisari Penyakit layu yang disebabkan oleh jamur Fusarium oxysporum f. sp.cubense (Foc) masih menjadi kendala dalam produksi pisang di seluruh dunia. Tujuan dari penelitian ini adalah untuk menguji viabilitas dan virulensi isolat Foc yang telah dipreservasi dengan liofilisasi selama 18 tahun. Penelitian menggunakan 19 isolat Foc yaitu Pekulo, Sukorejo, Kali Sepanjang, Kalibaru, Purwojoyo, Prembun, Mulyosari, Sidogede, Sidatan, Kemiri, Juwangen, A-17, A-11, PS-5, B4-3-1, Fo2.16.16., PS-10, A-2, dan Irja. Hasil penelitian menunjukkan bahwa 12 isolat Foc mampu tumbuh dengan baik pada medium PDA yaitu Pekulo, Sukorejo, Kali Sepanjang, Kalibaru, Purwojoyo, Prembun, Mulyosari, Sidatan, Juwangen, PS-5, B4-3-1, dan Fo2.16.16. 7 isolat tidak mampu tumbuh pada medium PDA yaitu Sidogede, Kemiri, A-17, A-11, PS-10, A-2, dan Irja. Enam isolat Foc yang diuji memiliki virulensi sangat tinggi dengan indeks keparahan penyakit berkisar antara 2,14–2,71. Tiga isolat yang lain memiliki virulensi tinggi dengan indeks keparahan penyakit berkisar antara 2,01–2,07. Sedangkan 3 isolat lainnya memiliki virulensi yang lebih rendah dengan indeks keparahan penyakit berkisar antara 1,39–1,67.
由尖孢镰刀菌(Fusarium oxysporum f. sp. cubense, Foc)引起的香蕉枯萎病(Wilt disease)至今仍是制约香蕉生产的一大病害。本研究的目的是检测冻干保存18年的Foc分离株的生存力和毒力。实验选用19株Foc分离株,分别为Pekulo、Sukorejo、Kali Sepanjang、Kalibaru、Purwojoyo、Prembun、Mulyosari、Sidogede、Sidatan、Kemiri、Juwangen、A-17、A-11、PS-5、B4-3-1、Fo2.16.16。PS-10, A-2和Irja。结果表明,12株菌株在PDA培养基上生长良好,分别为Pekulo、Sukorejo、Kali Sepanjang、Kalibaru、Purwojoyo、Prembun、Mulyosari、Sidatan、Juwangen、PS-5、B4-3-1和Fo2.16.16。同时,Sidogede、Kemiri、A-17、A-11、PS-10、A-2和Irja 7株分离株在PDA培养基上不能生长发育。致病性试验结果表明,6株Foc分离株具有很高的毒力,疾病严重程度指数约为2.14 ~ 2.71。其余3株毒株呈高毒力,疾病严重指数在2.01 ~ 2.07之间。另外3株毒力较弱的分离株的疾病严重程度指数在1.39 ~ 1.67之间。台湾尖孢镰刀菌(Foc),主要产自台湾,产自台湾,产自台湾。图juan dari penelitian, ini adalah, untuk, menguji, viabilitas and virulensis, Foc, yang, telah dipreservides, dengan, lifilisisselama, 18, tahun。Penelitian menggunakan 19 isolat Foc yaitu Pekulo, Sukorejo, Kali Sepanjang, Kalibaru, Purwojoyo, Prembun, Mulyosari, Sidogede, Sidatan, Kemiri, Juwangen, A-17, A-11, PS-5, B4-3-1, Fo2.16.16PS-10, A-2, dan Irja。yyitu Pekulo, Sukorejo, Kali Sepanjang, Kalibaru, Purwojoyo, Prembun, Mulyosari, Sidatan, Juwangen, PS-5, B4-3-1, dan Fo2.16.16。7分离株mampu tumbuh pada medium PDA yitu Sidogede, Kemiri, A-17, A-11, PS-10, A-2, dan Irja。中国农业科学学报(自然科学版)2,14-2,71。[j] .中国生物医学工程学报,2011,31(2):481 - 481。[j] .四川农业大学学报(自然科学版),1999,19(2):1 - 7。
{"title":"Viabilitas dan Virulensi Fusarium oxysporum f. sp. cubense yang Dipreservasi dengan Liofilisasi","authors":"Iqna Khayatina Rusli, Arif Wibowo, Christanti Sumardiyono","doi":"10.22146/JPTI.25715","DOIUrl":"https://doi.org/10.22146/JPTI.25715","url":null,"abstract":"Wilt disease caused by Fusarium oxysporum f. sp. cubense (Foc) is still pose as constraint to banana production all over the world. The objective of this research was to examine the viability and virulence of Foc isolates which had been preserved with lyophilization for 18 years. The experiment used 19 isolates of Foc, which were Pekulo, Sukorejo, Kali Sepanjang, Kalibaru, Purwojoyo, Prembun, Mulyosari, Sidogede, Sidatan, Kemiri, Juwangen, A-17, A-11, PS-5, B4-3-1, Fo2.16.16., PS-10, A-2, and Irja. The results showed that 12 isolates could grow well on PDA medium, namely Pekulo, Sukorejo, Kali Sepanjang, Kalibaru, Purwojoyo, Prembun, Mulyosari, Sidatan, Juwangen, PS-5, B4-3-1, and Fo2.16.16. Meanwhile, seven isolates (Sidogede, Kemiri, A-17, A-11, PS-10, A-2, and Irja) could not grow or develop on PDA medium. The result of pathogenicity test showed that six tested Foc isolates had very high virulences, with disease severity index of about 2.14–2.71. Other three isolates revealed high virulences with disease severity index around 2.01–2.07. Meanwhile, another three less virulent isolates demonstrated disease severity index in the range of 1.39–1.67. Intisari Penyakit layu yang disebabkan oleh jamur Fusarium oxysporum f. sp.cubense (Foc) masih menjadi kendala dalam produksi pisang di seluruh dunia. Tujuan dari penelitian ini adalah untuk menguji viabilitas dan virulensi isolat Foc yang telah dipreservasi dengan liofilisasi selama 18 tahun. Penelitian menggunakan 19 isolat Foc yaitu Pekulo, Sukorejo, Kali Sepanjang, Kalibaru, Purwojoyo, Prembun, Mulyosari, Sidogede, Sidatan, Kemiri, Juwangen, A-17, A-11, PS-5, B4-3-1, Fo2.16.16., PS-10, A-2, dan Irja. Hasil penelitian menunjukkan bahwa 12 isolat Foc mampu tumbuh dengan baik pada medium PDA yaitu Pekulo, Sukorejo, Kali Sepanjang, Kalibaru, Purwojoyo, Prembun, Mulyosari, Sidatan, Juwangen, PS-5, B4-3-1, dan Fo2.16.16. 7 isolat tidak mampu tumbuh pada medium PDA yaitu Sidogede, Kemiri, A-17, A-11, PS-10, A-2, dan Irja. Enam isolat Foc yang diuji memiliki virulensi sangat tinggi dengan indeks keparahan penyakit berkisar antara 2,14–2,71. Tiga isolat yang lain memiliki virulensi tinggi dengan indeks keparahan penyakit berkisar antara 2,01–2,07. Sedangkan 3 isolat lainnya memiliki virulensi yang lebih rendah dengan indeks keparahan penyakit berkisar antara 1,39–1,67. ","PeriodicalId":31599,"journal":{"name":"Jurnal Perlindungan Tanaman Indonesia","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42940207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nurviani Nurviani, S. Somowiyarjo, S. Sulandari, S. Subandiyah
Some extract of the banana plants parts have been identified to produce a compound that has efficacy as traditional medicine and human virus inhibitor. There was no previous report on the use of the banana flower as the plants antiviral sources for plant pathogen. The objective of this study was to identify the potency of two types of the banana flower as the Tobamovirus inhibitor substance. The antiviral was prepared from the extract of Ambon banana (Musa acuminata Colla) flower and Klutuk banana (Musa balbisiana Colla) flower, as the comparison extract of Mirabilis jalapa L. leaf which is widely known to have antiviral on various plant viruses was used. This study applied the Complete Randomized Design with three replications. Collected data includes the virus incubation period and the inhibitor power upon the local necrotic symptom on indicator plant. Results of this study showed that the extract of Ambon and Klutuk banana flower was able to inhibit the Tobamovirus with inhibition levels of 86.34% and 91.22%.IntisariBeberapa ekstrak bagian tanaman pisang diketahui memiliki kandungan senyawa yang berkhasiat sebagai obat tradisional dan zat yang dapat menghambat virus manusia. Belum pernah dilaporkan penggunaan bunga pisang sebagai sumber antiviral terhadap virus tumbuhan. Penelitian ini bertujuan untuk mengetahui potensi ekstrak bunga dua jenis pisang sebagai zat penghambat Tobamovirus. Antiviral disiapkan dari ekstrak bunga pisang Ambon (Musa acuminata Colla) dan pisang Klutuk (Musa balbisiana Colla), sebagai pembanding digunakan ekstrak daun Mirabilis jalapa L. yang sudah banyak diketahui mengandung antiviral pada berbagai virus tumbuhan. Penelitian ini dirancang menggunakan Rancangan Acak Lengkap dengan 3 ulangan. Data yang dikumpulkan meliputi masa inkubasi virus dan daya hambat gejala nekrotik lokal pada tanaman indikator. Hasil penelitian menunjukkan bahwa ekstrak bunga pisang jenis Ambon dan Klutuk mampu menghambat Tobamovirus dengan tingkat penghambatan sebesar 86,34% dan 91,22%.
{"title":"The Inhibition of Tobamovirus by Using the Extract of Banana Flower","authors":"Nurviani Nurviani, S. Somowiyarjo, S. Sulandari, S. Subandiyah","doi":"10.22146/jpti.31595","DOIUrl":"https://doi.org/10.22146/jpti.31595","url":null,"abstract":"Some extract of the banana plants parts have been identified to produce a compound that has efficacy as traditional medicine and human virus inhibitor. There was no previous report on the use of the banana flower as the plants antiviral sources for plant pathogen. The objective of this study was to identify the potency of two types of the banana flower as the Tobamovirus inhibitor substance. The antiviral was prepared from the extract of Ambon banana (Musa acuminata Colla) flower and Klutuk banana (Musa balbisiana Colla) flower, as the comparison extract of Mirabilis jalapa L. leaf which is widely known to have antiviral on various plant viruses was used. This study applied the Complete Randomized Design with three replications. Collected data includes the virus incubation period and the inhibitor power upon the local necrotic symptom on indicator plant. Results of this study showed that the extract of Ambon and Klutuk banana flower was able to inhibit the Tobamovirus with inhibition levels of 86.34% and 91.22%.IntisariBeberapa ekstrak bagian tanaman pisang diketahui memiliki kandungan senyawa yang berkhasiat sebagai obat tradisional dan zat yang dapat menghambat virus manusia. Belum pernah dilaporkan penggunaan bunga pisang sebagai sumber antiviral terhadap virus tumbuhan. Penelitian ini bertujuan untuk mengetahui potensi ekstrak bunga dua jenis pisang sebagai zat penghambat Tobamovirus. Antiviral disiapkan dari ekstrak bunga pisang Ambon (Musa acuminata Colla) dan pisang Klutuk (Musa balbisiana Colla), sebagai pembanding digunakan ekstrak daun Mirabilis jalapa L. yang sudah banyak diketahui mengandung antiviral pada berbagai virus tumbuhan. Penelitian ini dirancang menggunakan Rancangan Acak Lengkap dengan 3 ulangan. Data yang dikumpulkan meliputi masa inkubasi virus dan daya hambat gejala nekrotik lokal pada tanaman indikator. Hasil penelitian menunjukkan bahwa ekstrak bunga pisang jenis Ambon dan Klutuk mampu menghambat Tobamovirus dengan tingkat penghambatan sebesar 86,34% dan 91,22%. ","PeriodicalId":31599,"journal":{"name":"Jurnal Perlindungan Tanaman Indonesia","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44396308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Riska Awalia Putri, S. Sulandari, Christanti Sumardiyono, Triwidodo Arwiyanto
Tobamovirus is one of the important pathogen that decrease the quantity and quality of cigar tobacco cultivation. One of the alternative control of this disease is by using rhizosphere biological agents such as Bacillus spp., Streptomyces spp., and mycorrhiza. This study aims to determine the effectiveness and mechanism of the biological agents singly and in combination to induce cigar tobacco plant resistance of the F1 (TV38xG) variety in the greenhouse. The biological agents were applied on the root of tobacco during nursery and transplanting. The virus inoculation was performed 10 days after transplanting. The results showed that the treatment with Bacillus spp., Streptomyces spp., and mycorrhizal were capable to decrease disease incidence by delaying mosaic symptoms for 6-day in a single treatment and 3 days in combination treatment. The treatment of Bacillus spp., Streptomyces spp., and mycorrhiza both in single and in combination were capable to decrease the disease intensity for 26%, 23%, 15%, and 10%, respectively. Induced resistance was performed by increased peroxidase activity in the treatment of mycorrhiza as much as 29.27%, Bacillus spp. 20.65%, and Streptomyces spp. 11.10%. Protein analysis with SDS-PAGE of tobacco plants showed the difference in profile and size of protein bands. Protein bands measuring 17 kDa and 30 kDa were found in tobacco infected Tobamovirus but was not found in healthy tobacco. The single application of Bacillus spp., Streptomyces spp., and mycorrhiza on tobacco infected Tobamovirus increase plant growth including plant height as much as 53.71%, number of leaves 57%, stem diameter 29.40%, root length 60.77%, fresh weight of leaves 196.90%, and dry weight of leaf of 265.31%. IntisariTobamovirus merupakan salah satu patogen penting yang menyebabkan penurunan kuantitas dan kualitas pada budidaya tembakau cerutu. Salah satu alternatif pengendalian penyakit ini menggunakan agens hayati pengkoloni akar yaitu Bacillus spp., Streptomyces spp., dan Mikoriza. Penelitian bertujuan untuk mengetahui efektivitas dan mekanisme agens hayati secara tunggal dan kombinasi dalam menginduksi ketahanan tanaman tembakau cerutu varietas F1 (TV38xG) di rumah kaca. Agens hayati diaplikasikan pada perakaran tembakau pada saat pembibitan dan pindah tanam. Inokulasi virus dilakukan 10 hari setelah pindah tanam. Hasil penelitian menunjukkan bahwa perlakuan Bacillus spp., Streptomyces spp., dan Mikoriza dapat menurunkan insidensi penyakit dengan menunda munculnya gejala mosaik 6 hari pada perlakuan tunggal dan 3 hari pada perlakuan kombinasi. Perlakuan Bacillus spp., Streptomyces spp., dan Mikoriza secara tunggal, dan kombinasi dapat menurunkan intensitas penyakit mosaik berturut-turut 26%, 23%, 15%, dan 10%. Induksi ketahanan terbentuk melalui peningkatan aktivitas peroksidase pada perlakuan Mikoriza 29,27%, Bacillus spp. 20,65%, Streptomyces spp. 11,10%. Analisis protein dengan SDS-PAGE diperoleh adanya perbedaan profi dan ukuran
烟叶病毒是影响雪茄烟叶产量和质量的重要病原菌之一。另一种防治方法是使用根际生物制剂,如芽孢杆菌、链霉菌和菌根。本研究旨在确定生物制剂在温室内单独和联合诱导F1 (TV38xG)品种雪茄烟草植株抗性的有效性和机制。在烟草苗期和移栽期对烟草根部施用生物制剂。移植后10天接种病毒。结果表明,芽孢杆菌、链霉菌和菌根菌单独处理可使花叶病症状延迟6 d,联合处理可使花叶病症状延迟3 d,从而降低发病率。芽孢杆菌、链霉菌和菌根单独和联合处理可使病害强度分别降低26%、23%、15%和10%。通过提高过氧化物酶活性诱导抗性,菌根、芽孢杆菌和链霉菌分别提高了29.27%、20.65%和11.10%。利用SDS-PAGE对烟草植株进行蛋白质分析,发现蛋白质条带的形态和大小存在差异。在感染烟叶中发现了17 kDa和30 kDa的蛋白条带,而在健康烟叶中没有发现。单施芽孢杆菌、链霉菌和菌根对感染了托巴莫病毒的烟草,株高提高53.71%,叶数增加57%,茎粗增加29.40%,根长增加60.77%,叶片鲜重增加196.90%,干重增加265.31%。流行性感冒病毒(IntisariTobamovirus, merupakansalah)是一种病原体,它的致病性为:杨门叶病毒(menyebabkan penurunan kuantitas)和麻疹病毒(kualitas padadbudidaya tembakau cerutu)。Salah satu alternatifpengendalian penyakit ini menggunakan agenens hayati penkoloni akar yitu芽孢杆菌属,链霉菌属,dan Mikoriza。Penelitian bertujuan untuk mengetahui ekktivitas dan mekanisme代理hayati secara tungal dan kombinasi dalam menginduksi ketahanan tanaman tembakau cerutu变种F1 (TV38xG) di rumah kaca。代理hayati diapplikasikan pada perakaran tembakau pada pbidbitan pindah tanam。猪瘟病毒是一种传染性病毒。Hasil penelitian menunjukkan bahwa perlakuan芽孢杆菌,链霉菌,dan Mikoriza dapat menurunkan insidensi penyakit dengan menunda munculnya gejala mosaik 6 hari pada perlakuan tungal dan 3 hari pada perlakuan kombinasi。Perlakuan Bacillus spp, Streptomyces spp, dan Mikoriza secara tunggal, dan kombinasi dapat menurunkan intensitas penyakit mosaik berturut-turut 26%, 23%, 15%, dan 10%。Induksi ketahanan terbentuk melalui peningkatan aktivitas peroksidase pada perlakuan Mikoriza 29,27%,芽孢杆菌20,65%,链霉菌11,10%。分析蛋白dengan SDS-PAGE, diperoleh adanya perbedah和profi dan ukuran pita蛋白。白斑病蛋白,白斑病蛋白,白斑病蛋白,白斑病蛋白,白斑病蛋白,白斑病蛋白。应用卡西芽孢杆菌属,链霉菌属,小叶状芽孢杆菌属,小叶状芽孢杆菌属。Tinggi tanaman meningkat 53,71%, jumlah dawn 57%, diameter batang 29,40%, panjang akar 60,77%, berat segar dawn 196,90%, dan berat kering dawn 265,31%。
{"title":"Respons Ketahanan Tembakau terhadap Tobamovirus dengan Agens Hayati sebagai Induser","authors":"Riska Awalia Putri, S. Sulandari, Christanti Sumardiyono, Triwidodo Arwiyanto","doi":"10.22146/JPTI.31241","DOIUrl":"https://doi.org/10.22146/JPTI.31241","url":null,"abstract":"Tobamovirus is one of the important pathogen that decrease the quantity and quality of cigar tobacco cultivation. One of the alternative control of this disease is by using rhizosphere biological agents such as Bacillus spp., Streptomyces spp., and mycorrhiza. This study aims to determine the effectiveness and mechanism of the biological agents singly and in combination to induce cigar tobacco plant resistance of the F1 (TV38xG) variety in the greenhouse. The biological agents were applied on the root of tobacco during nursery and transplanting. The virus inoculation was performed 10 days after transplanting. The results showed that the treatment with Bacillus spp., Streptomyces spp., and mycorrhizal were capable to decrease disease incidence by delaying mosaic symptoms for 6-day in a single treatment and 3 days in combination treatment. The treatment of Bacillus spp., Streptomyces spp., and mycorrhiza both in single and in combination were capable to decrease the disease intensity for 26%, 23%, 15%, and 10%, respectively. Induced resistance was performed by increased peroxidase activity in the treatment of mycorrhiza as much as 29.27%, Bacillus spp. 20.65%, and Streptomyces spp. 11.10%. Protein analysis with SDS-PAGE of tobacco plants showed the difference in profile and size of protein bands. Protein bands measuring 17 kDa and 30 kDa were found in tobacco infected Tobamovirus but was not found in healthy tobacco. The single application of Bacillus spp., Streptomyces spp., and mycorrhiza on tobacco infected Tobamovirus increase plant growth including plant height as much as 53.71%, number of leaves 57%, stem diameter 29.40%, root length 60.77%, fresh weight of leaves 196.90%, and dry weight of leaf of 265.31%. IntisariTobamovirus merupakan salah satu patogen penting yang menyebabkan penurunan kuantitas dan kualitas pada budidaya tembakau cerutu. Salah satu alternatif pengendalian penyakit ini menggunakan agens hayati pengkoloni akar yaitu Bacillus spp., Streptomyces spp., dan Mikoriza. Penelitian bertujuan untuk mengetahui efektivitas dan mekanisme agens hayati secara tunggal dan kombinasi dalam menginduksi ketahanan tanaman tembakau cerutu varietas F1 (TV38xG) di rumah kaca. Agens hayati diaplikasikan pada perakaran tembakau pada saat pembibitan dan pindah tanam. Inokulasi virus dilakukan 10 hari setelah pindah tanam. Hasil penelitian menunjukkan bahwa perlakuan Bacillus spp., Streptomyces spp., dan Mikoriza dapat menurunkan insidensi penyakit dengan menunda munculnya gejala mosaik 6 hari pada perlakuan tunggal dan 3 hari pada perlakuan kombinasi. Perlakuan Bacillus spp., Streptomyces spp., dan Mikoriza secara tunggal, dan kombinasi dapat menurunkan intensitas penyakit mosaik berturut-turut 26%, 23%, 15%, dan 10%. Induksi ketahanan terbentuk melalui peningkatan aktivitas peroksidase pada perlakuan Mikoriza 29,27%, Bacillus spp. 20,65%, Streptomyces spp. 11,10%. Analisis protein dengan SDS-PAGE diperoleh adanya perbedaan profi dan ukuran ","PeriodicalId":31599,"journal":{"name":"Jurnal Perlindungan Tanaman Indonesia","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44596166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ralstonia syzygii subsp. syzygii that belong to Ralstonia solanacearum species complex is the cause of Sumateran disease of clove. The disease was reported to cause widespread devastation on clove plantings in Indonesia. One of the control strategies is to reduce the spread of the disease through early detection on clove seedlings. The study aimed to design the specific primers based on endoglucanase (egl) gene of R. syzygii subsp. syzygii as a tool for early diagnosis. The analyses were conducted on development of specific primers design using egl sequences retrieved from GenBank, Polymerase Chain Reaction (PCR), primers sensitivity and specificity test. The pair of primers UGMRss-F (5’-GCTCACCATCGC CAAGGACAGCG-3’) and UGMRss-R (5’-TTCGATCGAACGCCTGGTTGAGC-3’) could amplify R. syzygii subsp. syzygii at ~378 base pairs with 0.8 ng/µl minimum concentration of DNA. The primers was specific to R. syzygii subsp. syzygii but not to other bacterial species even in the same phylotype. IntisariRalstonia syzygii subsp. syzygii merupakan bakteri yang termasuk dalam kelompok Ralstonia solanacearum species complex yang menyebabkan penyakit Sumatera pada tanaman cengkih. Penyakit ini menyebabkan kerugian yang sangat besar dan sampai saat ini belum ditemukan cara pengendalian yang efektif. Salah satu upaya pencegahan penyakit adalah melalui deteksi dini dan mencegah penyebaran penyakit melalui peredaran bibit dari areal yang endemis. Penelitian ini bertujuan untuk merancang primer spesifik berbasis gen endoglukanase (egl) sebagai upaya deteksi dini penyakit Sumatera. Analisis yang dilakukan meliputi desain primer spesifik dengan menggunakan data sekuens gen egl dari GenBank, Polymerase Chain Reaction (PCR), uji kepekaan primer dan uji kekhususan primer. Desain primer yang berhasil dirancang terdiri dari UGMRss-F (5’- GCTCACCATCGCCAAGGACAGCG-3’) dan UGMRss-R (5’-TTC GATCGAACGCCTGGTTGAGC-3’) dengan amplikon ~378 pasang basa. Pada konsentrasi DNA 0,8 ng/µl, secara peka R. syzygii subsp. syzygii masih dapat teramplifikasi dengan baik. Primer ini juga hanya dapat mendeteksi R. syzygii subsp. syzygii dan tidak untuk bakteri lain bahkan pada filotipe yang sama.
裂谷菌亚种。丁香褐枯病是丁香褐枯病的病原菌。据报道,这种疾病对印度尼西亚的丁香种植造成了广泛的破坏。控制策略之一是通过对丁香幼苗的早期发现来减少疾病的传播。本研究旨在设计基于内切葡聚糖酶(egl)基因的特异性引物。Syzygii作为早期诊断的工具。利用GenBank检索到的egl序列进行特异性引物设计、聚合酶链反应(PCR)、引物敏感性和特异性测试。引物UGMRss-F(5′-GCTCACCATCGC CAAGGACAGCG-3′)和UGMRss-R(5′-TTCGATCGAACGCCTGGTTGAGC-3′)可扩增syzygii亚种。约378个碱基对,最低DNA浓度为0.8 ng/µl。引物对合胞菌亚种具有特异性。即使在同一种型中,对其他细菌也不具有Syzygii作用。印度石竹亚种。杨梅耶巴肯潘亚基苏门答腊帕达塔纳曼。Penyakit ini menyebabkan kerugian yang sangat besar dan sampai saat ini belum diemukan cara pengendalian yang efektif。Salah satu upaya penegahan penyakit adalah melalui deteksi dini dan menegah penyebaran penyakit melalui peredaran bibit dari areal yang endemis。Penelitian ini bertujuan untuk merancang引物特异的基原内糖化酶(egl) sebagai upaya deteksi dini penyakit sumat腊。分析杨迪拉坎梅利普蒂设计引物特异性邓安孟古纳坎数据sekuens gen egl dari GenBank,聚合酶链反应(PCR), uji kepekan引物和uji kekhususan引物。设计引物UGMRss-F(5′- GCTCACCATCGCCAAGGACAGCG-3′)和UGMRss-R(5′- ttc gatcgaacgcctggttggagc -3′)的扩增子~378 pasang bason。Pada konsentrasi DNA 0,8 ng/µl, secara peka R. syzygii subsp。登干银行的Syzygii masih基因扩增。引物ini juga handa dapat mendeteksi . syzygii subsp。Syzygii Dan tidak untuk bakteri lain bakhan pada filotipe Yang sama。
{"title":"Perancangan Primer Spesifik Subspesies Berbasis Gen Endoglukanase untuk Deteksi Ralstonia syzygii subsp. syzygii","authors":"Bambang Trianom, Triwidodo Arwiyanto, Tri Joko","doi":"10.22146/JPTI.32217","DOIUrl":"https://doi.org/10.22146/JPTI.32217","url":null,"abstract":"Ralstonia syzygii subsp. syzygii that belong to Ralstonia solanacearum species complex is the cause of Sumateran disease of clove. The disease was reported to cause widespread devastation on clove plantings in Indonesia. One of the control strategies is to reduce the spread of the disease through early detection on clove seedlings. The study aimed to design the specific primers based on endoglucanase (egl) gene of R. syzygii subsp. syzygii as a tool for early diagnosis. The analyses were conducted on development of specific primers design using egl sequences retrieved from GenBank, Polymerase Chain Reaction (PCR), primers sensitivity and specificity test. The pair of primers UGMRss-F (5’-GCTCACCATCGC CAAGGACAGCG-3’) and UGMRss-R (5’-TTCGATCGAACGCCTGGTTGAGC-3’) could amplify R. syzygii subsp. syzygii at ~378 base pairs with 0.8 ng/µl minimum concentration of DNA. The primers was specific to R. syzygii subsp. syzygii but not to other bacterial species even in the same phylotype. IntisariRalstonia syzygii subsp. syzygii merupakan bakteri yang termasuk dalam kelompok Ralstonia solanacearum species complex yang menyebabkan penyakit Sumatera pada tanaman cengkih. Penyakit ini menyebabkan kerugian yang sangat besar dan sampai saat ini belum ditemukan cara pengendalian yang efektif. Salah satu upaya pencegahan penyakit adalah melalui deteksi dini dan mencegah penyebaran penyakit melalui peredaran bibit dari areal yang endemis. Penelitian ini bertujuan untuk merancang primer spesifik berbasis gen endoglukanase (egl) sebagai upaya deteksi dini penyakit Sumatera. Analisis yang dilakukan meliputi desain primer spesifik dengan menggunakan data sekuens gen egl dari GenBank, Polymerase Chain Reaction (PCR), uji kepekaan primer dan uji kekhususan primer. Desain primer yang berhasil dirancang terdiri dari UGMRss-F (5’- GCTCACCATCGCCAAGGACAGCG-3’) dan UGMRss-R (5’-TTC GATCGAACGCCTGGTTGAGC-3’) dengan amplikon ~378 pasang basa. Pada konsentrasi DNA 0,8 ng/µl, secara peka R. syzygii subsp. syzygii masih dapat teramplifikasi dengan baik. Primer ini juga hanya dapat mendeteksi R. syzygii subsp. syzygii dan tidak untuk bakteri lain bahkan pada filotipe yang sama.","PeriodicalId":31599,"journal":{"name":"Jurnal Perlindungan Tanaman Indonesia","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43960524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Black pepper (Piper nigrum L.) is an important estate crops in Indonesia. Some pathogens that have been known to infect black pepper plants include fungi, nematodes and viruses. The stunting disease on black pepper plants was caused by Cucumber mosaic virus (CMV). Molecular detection using RT-PCR method showed that the samples were positively infected by CMV which were amplified by specific primers CMV 111 with bands of 111 bp in size. This virus can be carried by vegetative propagation material of plants. Many control strategies against this virus have been investigated, especially inducing plant resistance with chitosan. Chitosan is a natural biopolymer that play an important role in reducing disease incidence and severity and stimulate plant growth. The aim of this study was to figure out the inhibiting ability of chitosan solution against infection of stunting virus on black pepper seedlings through spraying applications. Chitosan treatments were prepared in concentrations of 0.5%, 0.75%, and 1%. The result showed that application of chitosan at all concentrations affected the decrease of disease incidence and intensity and improved plant growth with insignificant different amongst all treatments but significantly different with control. The highest decrease in incidence was found at 0.75% of chitosan concentration (26.37), while the highest decrease of intensity was expressed at 1% of chitosan (37.62). Application of chitosan also significantly affected to all parameters of plant growth either plant height or leaf diameter. Application of 1% of chitosan increased the percentage of plant growth rather than other treatments, with the increase of plant height 58.12 % and leaf diameter 54.74 %. IntisariLada (Piper nigrum L.) merupakan salah satu tanaman perkebunan penting di Indonesia. Beberapa patogen telah diketahui menginfeksi tanaman lada di antaranya jamur, nematoda, dan virus. Penyakit kerdil pada tanaman lada disebabkan oleh Cucumber mosaic virus (CMV). Deteksi molekuler menggunakan metode RT-PCR menunjukkan bahwa sampel positif terinfeksi CMV yang diamplifikasi menggunakan primer spesifik CMV 111 dengan ukuran pita band target 111 bp. Virus ini dapat terbawa bahan perbanyakan tanaman secara vegetatif. Banyak strategi pengendalian virus yang telah diuji, diantaranya induksi ketahanan tanaman dengan kitosan. Kitosan adalah biopolimer alami yang berperan dalam menurunkan insidensi dan intensitas penyakit dan menstimulasi pertumbuhan tanaman. Tujuan dari penelitian ini adalah untuk mengetahui kemampuan penghambatan dari larutan kitosan terhadap infeksi dari virus kerdil pada bibit lada dengan aplikasi penyemprotan. Konsentrasi kitosan yang digunakan adalah 0,5%; 0,75%; dan 1%. Hasil penelitian menunjukan bahwa apliksi kitosan pada semua konsentrasi berpengaruh dalam menurunkan insidensi dan intensitas penyakit dan meningkatkan pertumbuhan tanaman dengan tidak berbeda nyata di antara perlakuan tetapi berbeda nyata dengan kontrol. Penurunan
{"title":"The Effect of Chitosan Application against Plant Growth and Intensity of Stunting Disease on Black Pepper (Piper nigrum L.) Seedlings","authors":"E. Uge, S. Sulandari, S. Hartono, S. Somowiyarjo","doi":"10.22146/jpti.25453","DOIUrl":"https://doi.org/10.22146/jpti.25453","url":null,"abstract":"Black pepper (Piper nigrum L.) is an important estate crops in Indonesia. Some pathogens that have been known to infect black pepper plants include fungi, nematodes and viruses. The stunting disease on black pepper plants was caused by Cucumber mosaic virus (CMV). Molecular detection using RT-PCR method showed that the samples were positively infected by CMV which were amplified by specific primers CMV 111 with bands of 111 bp in size. This virus can be carried by vegetative propagation material of plants. Many control strategies against this virus have been investigated, especially inducing plant resistance with chitosan. Chitosan is a natural biopolymer that play an important role in reducing disease incidence and severity and stimulate plant growth. The aim of this study was to figure out the inhibiting ability of chitosan solution against infection of stunting virus on black pepper seedlings through spraying applications. Chitosan treatments were prepared in concentrations of 0.5%, 0.75%, and 1%. The result showed that application of chitosan at all concentrations affected the decrease of disease incidence and intensity and improved plant growth with insignificant different amongst all treatments but significantly different with control. The highest decrease in incidence was found at 0.75% of chitosan concentration (26.37), while the highest decrease of intensity was expressed at 1% of chitosan (37.62). Application of chitosan also significantly affected to all parameters of plant growth either plant height or leaf diameter. Application of 1% of chitosan increased the percentage of plant growth rather than other treatments, with the increase of plant height 58.12 % and leaf diameter 54.74 %. IntisariLada (Piper nigrum L.) merupakan salah satu tanaman perkebunan penting di Indonesia. Beberapa patogen telah diketahui menginfeksi tanaman lada di antaranya jamur, nematoda, dan virus. Penyakit kerdil pada tanaman lada disebabkan oleh Cucumber mosaic virus (CMV). Deteksi molekuler menggunakan metode RT-PCR menunjukkan bahwa sampel positif terinfeksi CMV yang diamplifikasi menggunakan primer spesifik CMV 111 dengan ukuran pita band target 111 bp. Virus ini dapat terbawa bahan perbanyakan tanaman secara vegetatif. Banyak strategi pengendalian virus yang telah diuji, diantaranya induksi ketahanan tanaman dengan kitosan. Kitosan adalah biopolimer alami yang berperan dalam menurunkan insidensi dan intensitas penyakit dan menstimulasi pertumbuhan tanaman. Tujuan dari penelitian ini adalah untuk mengetahui kemampuan penghambatan dari larutan kitosan terhadap infeksi dari virus kerdil pada bibit lada dengan aplikasi penyemprotan. Konsentrasi kitosan yang digunakan adalah 0,5%; 0,75%; dan 1%. Hasil penelitian menunjukan bahwa apliksi kitosan pada semua konsentrasi berpengaruh dalam menurunkan insidensi dan intensitas penyakit dan meningkatkan pertumbuhan tanaman dengan tidak berbeda nyata di antara perlakuan tetapi berbeda nyata dengan kontrol. Penurunan ","PeriodicalId":31599,"journal":{"name":"Jurnal Perlindungan Tanaman Indonesia","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48439511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Fatmawati Kalau, F. Wagiman, Witjaksono Witjaksono
Determination of tolerance threshold of golden snail (Pomacea canaliculata) infestation in irrigated rice had been studied at Sumbersari Village, Moyudan District, Sleman Regency, Yogyakarta Special Teritory, in the growing season of June to October 2016. The water depth during the experiment was maintained at 1 cm. The snails of 2−3 cm in length were infested on rice plots at various ages. The snails were infested on rice plots with density rates of (1) 0, 1, 2, 3, 5, 15, 30, (2) 0, 2, 3, 5, 10, 15, 30, and (3) 0, 3, 5, 10, 15, 20, 30 individuals/m2 at (1) 0 day after planting (DAP), (2) 7 and 14 DAP, and (3) 28 DAP, respectively. These treatments were replicated three times and arranged in the randomized complete block design (RCBD). In this study the tolerance threshold is defined as the highest snail density which causes no significant effect on rice damage and yield loss as compared to control or to the lowest snail density. Therefore, determination of the tolerance threshold was based on the significant difference of damage severity, panicle number per hill, and harvesting dry-weight of rice grain. Results showed that significant rice damage occurred on age of 0, 7, and 14 DAP olds, while on age of 21 and 28 DAP the rice showed no damage. More severe damage occurred to the younger rice. The tolerance threshold values of the snail on rice plots with 1 cm water depth at 0 DAP old was approximately 2 individuals/m2 while at age of 7 and 14 DAP were approximately 3 individuals/m2, respectively. IntisariPenentuan ambang toleransi serangan keong emas (Pomacea canaliculata) pada padi sawah telah dikaji di Desa Sumbersari, Kecamatan Moyudan, Kabupaten Sleman, Daerah Istimewa Yogyakarta dalam musim tanam Juni–Oktober 2016. Kedalaman air selama percobaan berlangsung dikondisikan sedalam 1 cm. Keong berukuran 2−3 cm diinfestasikan pada berbagai umur padi. Keong dengan kepadatan (1) 0, 1, 2, 3, 5, 15, 30; (2) 0, 2, 3, 5, 10, 15, 30; dan (3) 0, 3, 5, 10, 15, 20, 30 ekor/m2 diinfestasikan berturut-turut pada plot padi (1) saat tanam (0 hari setelah tanaman (HST)), (2) umur 7 dan 14 HST, dan (3) serta umur 21, dan 28 HST. Perlakuan diulang tiga kali dan diatur dalam rancangan randomized complete block design (RCBD). Dalam penelitian ini ambang toleransi didefinisikan sebagai kepadatan keong tertinggi yang menyebabkan kerusakan tanaman padi dan kehilangan hasil tidak berbeda signifikan dengan kontrol atau dengan kepadatan populasi terendah. Oleh karena itu nilai ambang toleranasi ditentukan berdasarkan signifikansi perbedaan kerusakan tanaman, jumlah bulir per rumpun, dan berat kering panen gabah. Hasil kajian menunjukkan bahwa kerusakan signifikan terjadi pada padi umur 0, 7, dan 14 hari setelah tanam (HST), sedangkan pada umur 21 dan 28 HST padi tidak menunjukkan kerusakan. Kerusakan semakin parah pada padi semakin muda. Pada kondisi kedalaman air 1 cm, nilai ambang toleransi keong mas pada padi umur 0 HST sekitar 2 ekor/m2 sedangkan pada umur 7 dan 1
2016年6 - 10月,在日惹特别行政区Sleman Regency Moyudan区Sumbersari村研究了灌溉水稻对金螺(Pomacea canaliculata)侵染的耐受阈值测定。实验过程中水深保持在1cm。钉螺在不同年龄的稻田中孳生,长度为2 ~ 3 cm。分别在种植后(1)0天、(2)7天、14天、(3)28天、(1)0、1、2、3、5、15、30、(2)0、2、3、5、10、15、30、(3)0、3、5、10、15、20、30只/m2的稻田田块上进行钉螺侵染。这些治疗重复了三次,并按随机完全区设计(RCBD)进行安排。在本研究中,耐受阈值定义为与对照相比,对水稻危害和产量损失没有显著影响的最高蜗牛密度或最低蜗牛密度。因此,确定耐受阈值的依据是水稻的危害程度、每山穗数和收获干重的显著差异。结果表明,水稻在0、7、14 DAP龄发生了显著的病害,而在21、28 DAP龄未发生病害。幼龄水稻受到的损害更为严重。0 DAP龄时,1 cm水深稻田田块上螺的耐受阈值约为2只/m2, 7 DAP龄和14 DAP龄时,螺的耐受阈值约为3只/m2。IntisariPenentuan ambang toleransi serangan keong emas (Pomacea canaliculata) padi padi sawah telah dikaji di Desa Sumbersari, Kecamatan Moyudan, Kabupaten Sleman, Daerah Istimewa日惹dalam穆斯林tanam 2016年7月- 10月。Kedalaman air selama percobaan berlangsung dikondisikan sedalam 1厘米。Keong berukuran 2 - 3 cm diinfestasikan pada berbagai umur padi。姜登根的研究进展(1)0,1,2,3,5,15,30;(2) 0,2,3,5,10,15,30;丹(3)0,3,5,10,15,20,30 ekor/m2 diinfestasikan berturut-turut padi (1) saat tanam (0 hari setelah tanaman (HST)), (2) umur 7丹14 HST,丹(3)serta umur 21,丹28 HST。Perlakuan diulang tiga kali dan diatur dalam ranangan随机完全区设计(RCBD)。Dalam penelitian ini ambang容忍,didefinisikan sebagai kepadatan kepadatan kepadatan kepadatan kepadatan kepadatan kepadatan kepadatan kepadatan kepadatan kepadatan berbeda显著,dengan控制,dengan kepadatan populasi terendah。Oleh karena itu nilai ambang toleranasi ditentukan berdasarkan signfikansi perbedaan kerusakan tanaman, jumlah buliper rumpun, dan beringpanen gabah。Hasil kajian menunjukkan bahwa kerusakan signfikan terjadi padpadi umur 0,7, dan 14 hari setelah tanam (HST), sedangkan padumur 21 dan 28 HST padi tidak menunjukkan kerusakan。Kerusakan semakin parah padi padi semakin muda。Pada kondisi kedalaman空气1厘米,nilai ambang容忍keong mas Pada padi umur 0 HST sekitar 2 ekor/m2 sedangkan Pada umur 7丹14 HST sekitar 3 ekor/m2。
{"title":"Determination of Tolerance Threshold Level of Golden Snail (Pomacea canaliculata) in Irrigated Rice","authors":"Fatmawati Kalau, F. Wagiman, Witjaksono Witjaksono","doi":"10.22146/jpti.24820","DOIUrl":"https://doi.org/10.22146/jpti.24820","url":null,"abstract":"Determination of tolerance threshold of golden snail (Pomacea canaliculata) infestation in irrigated rice had been studied at Sumbersari Village, Moyudan District, Sleman Regency, Yogyakarta Special Teritory, in the growing season of June to October 2016. The water depth during the experiment was maintained at 1 cm. The snails of 2−3 cm in length were infested on rice plots at various ages. The snails were infested on rice plots with density rates of (1) 0, 1, 2, 3, 5, 15, 30, (2) 0, 2, 3, 5, 10, 15, 30, and (3) 0, 3, 5, 10, 15, 20, 30 individuals/m2 at (1) 0 day after planting (DAP), (2) 7 and 14 DAP, and (3) 28 DAP, respectively. These treatments were replicated three times and arranged in the randomized complete block design (RCBD). In this study the tolerance threshold is defined as the highest snail density which causes no significant effect on rice damage and yield loss as compared to control or to the lowest snail density. Therefore, determination of the tolerance threshold was based on the significant difference of damage severity, panicle number per hill, and harvesting dry-weight of rice grain. Results showed that significant rice damage occurred on age of 0, 7, and 14 DAP olds, while on age of 21 and 28 DAP the rice showed no damage. More severe damage occurred to the younger rice. The tolerance threshold values of the snail on rice plots with 1 cm water depth at 0 DAP old was approximately 2 individuals/m2 while at age of 7 and 14 DAP were approximately 3 individuals/m2, respectively. IntisariPenentuan ambang toleransi serangan keong emas (Pomacea canaliculata) pada padi sawah telah dikaji di Desa Sumbersari, Kecamatan Moyudan, Kabupaten Sleman, Daerah Istimewa Yogyakarta dalam musim tanam Juni–Oktober 2016. Kedalaman air selama percobaan berlangsung dikondisikan sedalam 1 cm. Keong berukuran 2−3 cm diinfestasikan pada berbagai umur padi. Keong dengan kepadatan (1) 0, 1, 2, 3, 5, 15, 30; (2) 0, 2, 3, 5, 10, 15, 30; dan (3) 0, 3, 5, 10, 15, 20, 30 ekor/m2 diinfestasikan berturut-turut pada plot padi (1) saat tanam (0 hari setelah tanaman (HST)), (2) umur 7 dan 14 HST, dan (3) serta umur 21, dan 28 HST. Perlakuan diulang tiga kali dan diatur dalam rancangan randomized complete block design (RCBD). Dalam penelitian ini ambang toleransi didefinisikan sebagai kepadatan keong tertinggi yang menyebabkan kerusakan tanaman padi dan kehilangan hasil tidak berbeda signifikan dengan kontrol atau dengan kepadatan populasi terendah. Oleh karena itu nilai ambang toleranasi ditentukan berdasarkan signifikansi perbedaan kerusakan tanaman, jumlah bulir per rumpun, dan berat kering panen gabah. Hasil kajian menunjukkan bahwa kerusakan signifikan terjadi pada padi umur 0, 7, dan 14 hari setelah tanam (HST), sedangkan pada umur 21 dan 28 HST padi tidak menunjukkan kerusakan. Kerusakan semakin parah pada padi semakin muda. Pada kondisi kedalaman air 1 cm, nilai ambang toleransi keong mas pada padi umur 0 HST sekitar 2 ekor/m2 sedangkan pada umur 7 dan 1","PeriodicalId":31599,"journal":{"name":"Jurnal Perlindungan Tanaman Indonesia","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43982721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study aims to determine the type and potency of egg parasitoid in controlling brown planthopper (Nilaparvata lugens Stal.) pests in Banyumas regency after the pest’s explosion. This research has been conducted in five districts of the endemic area of brown planthopper in Banyumas Regency, i.e. in Jatilawang, Cilongok, Kebasen, Sumpiuh, and Kembaran. Each of the districts was taken 5 sample villages. The testing and calculation of the level of parasitization were done in the laboratory of Plant Protection, Faculty of Agriculture, University of Jenderal Soedirman, Purwokerto. The research used nest plot design, where the first factor was the district and the second factor was the village. Village nested in district. The data were analyzed using F 5% test, followed by 5% DMRT, if there were any differences found . The results of the study were as follows: (1) The parasitoids found in Banyumas Regency were Gonatocerus sp. and Oligosita sp. with the ability to parasite 26.8−64.73%, and 1.82−31.40%; (2) the presence parasitoid has the potency to suppress the intensity of brown planthopper attack on the vegetative phase, the intensity of attacks ranged between 6.96−23.58%, with brown planthopper population ranged from 0.84 to 27.36 individuals per hill. IntisariPenelitian ini bertujuan untuk mengetahui jenis dan potensi parasitoid telur dalam mengendalikan hama wereng batang coklat (Nilaparvata lugens Stal.) di Kabupaten Banyumas pasca terjadinya ledakan. Penelitian ini dilaksanakan di lima kecamatan daerah endemik wereng batang cokelat di wilayah Kabupaten Banyumas yaitu Kecamatan Jatilawang, Cilongok, Kebasen, Sumpiuh, Kembaran. Masing-masing kecamatan diambil 5 desa sampel. Pengujian dan penghitungan tingkat pemarasitan dilakukan di laboratorium Perlindungan Tanaman Fakultas Pertanian, Universitas Jenderal Soedirman, Purwokerto. Penelitian menggunakan rancangan petak tersarang, dengan kecamatan sebagai faktor pertama dan desa sebagai faktor kedua. Desa tersarang pada kecamatan. Data dianalisis menggunakan uji F 5%, apabila ada perbedaan dilanjutkan dengan uji banding ganda DMRT 5 %. Adapun hasil penelitian adalah sebagai berikut: (1) Parasitoid yang ditemukan di Kabupaten Banyumas adalah Gonatocerus sp. dan Oligosita sp. dengan kemampuan memarasit 26,8−64,73%, dan sebesar 1,82−31,40 %, (2) keberadaan parasitoid berpotensi menekan intensitas serangan hama wereng batang cokelat pada fase vegetatif, intensitas serangan berkisar antara 6,96−23,58%, dengan populasi wereng batang cokelat berkisar 0,84−27,36 individu per rumpun.
{"title":"Potensi Parasitoid Telur dalam Mengendalikan Wereng Batang Cokelat (Nilaparvata lugens Stal.) Pasca Ledakan Populasi di Kabupaten Banyumas","authors":"E. Minarni, Agus Suyanto, Kartini Kartini","doi":"10.22146/JPTI.28886","DOIUrl":"https://doi.org/10.22146/JPTI.28886","url":null,"abstract":"This study aims to determine the type and potency of egg parasitoid in controlling brown planthopper (Nilaparvata lugens Stal.) pests in Banyumas regency after the pest’s explosion. This research has been conducted in five districts of the endemic area of brown planthopper in Banyumas Regency, i.e. in Jatilawang, Cilongok, Kebasen, Sumpiuh, and Kembaran. Each of the districts was taken 5 sample villages. The testing and calculation of the level of parasitization were done in the laboratory of Plant Protection, Faculty of Agriculture, University of Jenderal Soedirman, Purwokerto. The research used nest plot design, where the first factor was the district and the second factor was the village. Village nested in district. The data were analyzed using F 5% test, followed by 5% DMRT, if there were any differences found . The results of the study were as follows: (1) The parasitoids found in Banyumas Regency were Gonatocerus sp. and Oligosita sp. with the ability to parasite 26.8−64.73%, and 1.82−31.40%; (2) the presence parasitoid has the potency to suppress the intensity of brown planthopper attack on the vegetative phase, the intensity of attacks ranged between 6.96−23.58%, with brown planthopper population ranged from 0.84 to 27.36 individuals per hill. IntisariPenelitian ini bertujuan untuk mengetahui jenis dan potensi parasitoid telur dalam mengendalikan hama wereng batang coklat (Nilaparvata lugens Stal.) di Kabupaten Banyumas pasca terjadinya ledakan. Penelitian ini dilaksanakan di lima kecamatan daerah endemik wereng batang cokelat di wilayah Kabupaten Banyumas yaitu Kecamatan Jatilawang, Cilongok, Kebasen, Sumpiuh, Kembaran. Masing-masing kecamatan diambil 5 desa sampel. Pengujian dan penghitungan tingkat pemarasitan dilakukan di laboratorium Perlindungan Tanaman Fakultas Pertanian, Universitas Jenderal Soedirman, Purwokerto. Penelitian menggunakan rancangan petak tersarang, dengan kecamatan sebagai faktor pertama dan desa sebagai faktor kedua. Desa tersarang pada kecamatan. Data dianalisis menggunakan uji F 5%, apabila ada perbedaan dilanjutkan dengan uji banding ganda DMRT 5 %. Adapun hasil penelitian adalah sebagai berikut: (1) Parasitoid yang ditemukan di Kabupaten Banyumas adalah Gonatocerus sp. dan Oligosita sp. dengan kemampuan memarasit 26,8−64,73%, dan sebesar 1,82−31,40 %, (2) keberadaan parasitoid berpotensi menekan intensitas serangan hama wereng batang cokelat pada fase vegetatif, intensitas serangan berkisar antara 6,96−23,58%, dengan populasi wereng batang cokelat berkisar 0,84−27,36 individu per rumpun.","PeriodicalId":31599,"journal":{"name":"Jurnal Perlindungan Tanaman Indonesia","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44175589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The diamondback moth, Plutella xylostella L. (Lepidoptera: Plutellidae) is one of the major pests of the Brassicaceae family. Presently, farmers mostly use insecticide to control this pest. The improper use of insecticide, however, may lead to target pest resistance. Resistance to pyrethroid (cypermethrin) insecticide cases have been widely reported. This research aimed to know whether the farmers use cypermethrin based on the recommended practices and to determine the resistance level of P. xylostella collected from Tarus, Noelbaki, Oesao and Pukdale Villages collected from the Kupang District, East Nusa Tenggara, which were then compared to the population of Cangkringan (Sleman, Yogyakarta) as a susceptible population. The survey showed that these four locations in Kupang, used insecticides intensively and did the mixing of two or three insecticides. Insecticide application in these four locations was mostly not scheduled as recommended on the label of insecticides. The susceptibility test showed that the RR (resistance ratio) value from Kupang (Tarus = 9.2, Noelbaki = 7.2, Oesao 7.3, and Pukdale = 3.8, respecively) was higher than susceptible population (Cangkringan = 1.0). Therefore, P. xylostella larvae collected from Kupang has been resistant to cypermethrin. IntisariPlutella xylostella L. merupakan salah satu hama utama pada tanaman Famili Brassicaceae (Cruciferae). Pengendalian dengan insektisida masih menjadi andalan petani di lapangan. Dampak negatif dari insektisida seperti resistensi, resurgensi dan munculnya hama sekunder tidak membuat efek jera karena minimnya pengetahuan tentang dampak negatif tersebut. Resistensi terhadap golongan piretroid sudah banyak dilaporkan. Salah satunya sipermetrin. Tujuan dari penelitian ini untuk melihat cara penggunaan insektisida oleh petani dan tingkat kepekaan dari populasi lapang asal Tarus, Noelbaki, Oesao, dan Pukdale (Kupang, NTT) dibandingkan dengan populasi Cangkringan (Sleman, DIY) terhadap insektisida sipermetrin. Data survei tentang cara penggunaan insektisida oleh petani diketahui bahwa penggunaan insektisida di keempat lokasi tersebut cukup intensif dan terjadi pencampuran insektisida yang tidak kompatibel. Penyemprotan dilakukan secara rutin di Desa Tarus; di Desa Noelbaki dan Oesao ada yang secara rutin dan ada yang kondisional; sedangkan penyemprotan secara kondisional di Desa Pukdale. Data uji kepekaan menunjukkan bahwa P. xylostella asal Kupang telah resisten terhadap sipermetrin dengan nilai RR (rasio resistensisi) tertinggi dari populasi Tarus (9,2), Noelbaki (7,2), Oesao (7,3), Pukdale (3,8) dibandingkan dengan populasi peka asal Cangkringan.
{"title":"Resistance Level of Plutella xylostella L. (Lepidoptera: Plutellidae) on Cypermethrin in the Regency of Kupang","authors":"N. Kotta, Y. Trisyono, A. Wijonarko","doi":"10.22146/jpti.24750","DOIUrl":"https://doi.org/10.22146/jpti.24750","url":null,"abstract":"The diamondback moth, Plutella xylostella L. (Lepidoptera: Plutellidae) is one of the major pests of the Brassicaceae family. Presently, farmers mostly use insecticide to control this pest. The improper use of insecticide, however, may lead to target pest resistance. Resistance to pyrethroid (cypermethrin) insecticide cases have been widely reported. This research aimed to know whether the farmers use cypermethrin based on the recommended practices and to determine the resistance level of P. xylostella collected from Tarus, Noelbaki, Oesao and Pukdale Villages collected from the Kupang District, East Nusa Tenggara, which were then compared to the population of Cangkringan (Sleman, Yogyakarta) as a susceptible population. The survey showed that these four locations in Kupang, used insecticides intensively and did the mixing of two or three insecticides. Insecticide application in these four locations was mostly not scheduled as recommended on the label of insecticides. The susceptibility test showed that the RR (resistance ratio) value from Kupang (Tarus = 9.2, Noelbaki = 7.2, Oesao 7.3, and Pukdale = 3.8, respecively) was higher than susceptible population (Cangkringan = 1.0). Therefore, P. xylostella larvae collected from Kupang has been resistant to cypermethrin. IntisariPlutella xylostella L. merupakan salah satu hama utama pada tanaman Famili Brassicaceae (Cruciferae). Pengendalian dengan insektisida masih menjadi andalan petani di lapangan. Dampak negatif dari insektisida seperti resistensi, resurgensi dan munculnya hama sekunder tidak membuat efek jera karena minimnya pengetahuan tentang dampak negatif tersebut. Resistensi terhadap golongan piretroid sudah banyak dilaporkan. Salah satunya sipermetrin. Tujuan dari penelitian ini untuk melihat cara penggunaan insektisida oleh petani dan tingkat kepekaan dari populasi lapang asal Tarus, Noelbaki, Oesao, dan Pukdale (Kupang, NTT) dibandingkan dengan populasi Cangkringan (Sleman, DIY) terhadap insektisida sipermetrin. Data survei tentang cara penggunaan insektisida oleh petani diketahui bahwa penggunaan insektisida di keempat lokasi tersebut cukup intensif dan terjadi pencampuran insektisida yang tidak kompatibel. Penyemprotan dilakukan secara rutin di Desa Tarus; di Desa Noelbaki dan Oesao ada yang secara rutin dan ada yang kondisional; sedangkan penyemprotan secara kondisional di Desa Pukdale. Data uji kepekaan menunjukkan bahwa P. xylostella asal Kupang telah resisten terhadap sipermetrin dengan nilai RR (rasio resistensisi) tertinggi dari populasi Tarus (9,2), Noelbaki (7,2), Oesao (7,3), Pukdale (3,8) dibandingkan dengan populasi peka asal Cangkringan. ","PeriodicalId":31599,"journal":{"name":"Jurnal Perlindungan Tanaman Indonesia","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48583245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}