Penyakit busuk bulir bakteri merupakan penyakit penting pada tanaman padi yang disebabkan oleh bakteri Burkholderia glumae dan menyebabkan kehilangan hasil hingga 70%. Umumnya pengendalian penyakit ini dianjurkan melalui perlakuan benih menggunakan berbagai agens hayati, di antaranya aktinomiset penghasil senyawa bioaktif antibakteri. Penelitian ini bertujuan mendapatkan galur aktinomiset yang berpotensi sebagai pengendali penyakit busuk bulir bakteri yang disebabkan oleh B. glumae. Tahapan penelitian mencakup isolasi dan skrining aktinomiset yang berpotensi sebagai agens hayati terhadap B. glumae, uji fitotoksisitas aktinomiset terhadap benih padi, dan uji keefektifan aktinomiset dalam mengendalikan B. glumae pada padi fase pembibitan. Aktinomiset yang berhasil diisolasi dari tanaman padi dan rizosfer ialah sebanyak 40 isolat dan 17 di antaranya tidak berpotensi sebagai patogen terhadap tanaman maupun mamalia. Potensi penekanan galur aktinomiset berdasarkan uji antibiosis menghasilkan 7 galur aktinomiset yang mampu menekan perkembangan B. glumae dan sebanyak 5 galur memiliki zona hambatan lebih dari 2 mm, dan tidak bersifat fitotoksik terhadap tanaman padi dan memiliki kemampuan sebagai pemacu pertumbuhan. Galur aktinomiset ini berpengaruh terhadap pertumbuhan dan menekan derajat infeksi B. glumae. Aktinomiset galur ST1, ST27, dan BT23 efektif menekan penyakit busuk bulir bakteri padi pada fase pembibitan.
{"title":"Biological Control of Bacterial Grain Rot Disease Caused by Burkholderia glumae Using Actinomycetes","authors":"Nurmujahidin, G. Giyanto, Dadang","doi":"10.14692/jfi.19.2.63-73","DOIUrl":"https://doi.org/10.14692/jfi.19.2.63-73","url":null,"abstract":"Penyakit busuk bulir bakteri merupakan penyakit penting pada tanaman padi yang disebabkan oleh bakteri Burkholderia glumae dan menyebabkan kehilangan hasil hingga 70%. Umumnya pengendalian penyakit ini dianjurkan melalui perlakuan benih menggunakan berbagai agens hayati, di antaranya aktinomiset penghasil senyawa bioaktif antibakteri. Penelitian ini bertujuan mendapatkan galur aktinomiset yang berpotensi sebagai pengendali penyakit busuk bulir bakteri yang disebabkan oleh B. glumae. Tahapan penelitian mencakup isolasi dan skrining aktinomiset yang berpotensi sebagai agens hayati terhadap B. glumae, uji fitotoksisitas aktinomiset terhadap benih padi, dan uji keefektifan aktinomiset dalam mengendalikan B. glumae pada padi fase pembibitan. Aktinomiset yang berhasil diisolasi dari tanaman padi dan rizosfer ialah sebanyak 40 isolat dan 17 di antaranya tidak berpotensi sebagai patogen terhadap tanaman maupun mamalia. Potensi penekanan galur aktinomiset berdasarkan uji antibiosis menghasilkan 7 galur aktinomiset yang mampu menekan perkembangan B. glumae dan sebanyak 5 galur memiliki zona hambatan lebih dari 2 mm, dan tidak bersifat fitotoksik terhadap tanaman padi dan memiliki kemampuan sebagai pemacu pertumbuhan. Galur aktinomiset ini berpengaruh terhadap pertumbuhan dan menekan derajat infeksi B. glumae. Aktinomiset galur ST1, ST27, dan BT23 efektif menekan penyakit busuk bulir bakteri padi pada fase pembibitan.","PeriodicalId":31619,"journal":{"name":"Jurnal Fitopatologi Indonesia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48885798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yellow mosaic disease was reported for the first time in Indonesia in 2008. Its infection on yard long bean caused significant yield loss. Mungbean yellow mosaic India virus (MYMIV), member of genus Begomovirus was identified as the main causal agent. Specific and accurate detection is important for disease monitoring as part of disease management strategy. The aim of this study was to construct specific primer pairs for quick and robust detection of MYMIV using polymerase chain reaction method. A pair of primers MY1/MY2 was designed in this study to amplify part of MYMIV coat protein. In silico and in vitro test showed that MY1/MY2 primers specifically amplified MYMIV.
{"title":"Development of Specific Detection for Mungbean Yellow Mosaic India Virus Infecting Yard Long Bean in Java, Indonesia","authors":"S. Nurulita, K. Mutaqin, S. Hidayat","doi":"10.14692/jfi.19.2.83-88","DOIUrl":"https://doi.org/10.14692/jfi.19.2.83-88","url":null,"abstract":"Yellow mosaic disease was reported for the first time in Indonesia in 2008. Its infection on yard long bean caused significant yield loss. Mungbean yellow mosaic India virus (MYMIV), member of genus Begomovirus was identified as the main causal agent. Specific and accurate detection is important for disease monitoring as part of disease management strategy. The aim of this study was to construct specific primer pairs for quick and robust detection of MYMIV using polymerase chain reaction method. A pair of primers MY1/MY2 was designed in this study to amplify part of MYMIV coat protein. In silico and in vitro test showed that MY1/MY2 primers specifically amplified MYMIV.","PeriodicalId":31619,"journal":{"name":"Jurnal Fitopatologi Indonesia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45360702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ni Made Susun Parwanayoni Made Susun, Nyoman Darsini
Potensi keanekaragaman hayati flora di Indonesia belum dimanfaatkan secara maksimal sebagai pestisida nabati yang ramah lingkungan, dan mengurangi penggunaan pestisida sintetis pada tanaman. Daun Mansoa alliacea dan Allamanda cathartica memiliki potensi sebagai pestisida nabati karena secara in vitro mampu menghambat Athelia rolfsii, penyebab penyakit busuk batang pada tanaman kedelai. Penelitian dilakukan pada skala lapangan untuk menguji formula aplikasi ekstrak daun M.allicea dan A. cathartica yang berpengaruh terhadap perkembangan penyakit busuk batang, pertumbuhan dan produktivitas tanaman kedelai. Tahapan penelitian meliputi: ekstraksi, pembuatan formula dengan mencampurkan ekstrak daun M.allicea dan A. cathartica, aplikasi formula pada tanaman kedelai, pengamatan, dan pemanenan. Formula ekstrak 1.5% daun M. alliacea dan A. cathartica direkomendasikan untuk diaplikasikan karena dapat menekan penyakit busuk batang, meningkatkan pertumbuhan tanaman dan hasil panen di lapangan.
{"title":"Mansoa alliacea and Allamanda cathartica Extract Formula for The Control of Stem Rot Disease on Soybean Plants","authors":"Ni Made Susun Parwanayoni Made Susun, Nyoman Darsini","doi":"10.14692/jfi.19.2.57-62","DOIUrl":"https://doi.org/10.14692/jfi.19.2.57-62","url":null,"abstract":"Potensi keanekaragaman hayati flora di Indonesia belum dimanfaatkan secara maksimal sebagai pestisida nabati yang ramah lingkungan, dan mengurangi penggunaan pestisida sintetis pada tanaman. Daun Mansoa alliacea dan Allamanda cathartica memiliki potensi sebagai pestisida nabati karena secara in vitro mampu menghambat Athelia rolfsii, penyebab penyakit busuk batang pada tanaman kedelai. Penelitian dilakukan pada skala lapangan untuk menguji formula aplikasi ekstrak daun M.allicea dan A. cathartica yang berpengaruh terhadap perkembangan penyakit busuk batang, pertumbuhan dan produktivitas tanaman kedelai. Tahapan penelitian meliputi: ekstraksi, pembuatan formula dengan mencampurkan ekstrak daun M.allicea dan A. cathartica, aplikasi formula pada tanaman kedelai, pengamatan, dan pemanenan. Formula ekstrak 1.5% daun M. alliacea dan A. cathartica direkomendasikan untuk diaplikasikan karena dapat menekan penyakit busuk batang, meningkatkan pertumbuhan tanaman dan hasil panen di lapangan.","PeriodicalId":31619,"journal":{"name":"Jurnal Fitopatologi Indonesia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45654485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Xanthomonas campestris pv. campestris merupakan bakteri penyebab penyakit busuk hitam (black rot) pada kubis. Petani masih mengandalkan pestitida sintetis azoksistrobin dan difenokonazol untuk mengendalikan penyakit pada tanaman kubis yang dikhawatirkan akan berdampak pada keanekaragaman X. campestris pv. campestris. Tujuan penelitian ialah mendapatkan data keanekaragaman secara genetik dan fenotipik isolat bakteri X. campestris pv. campestris, serta mendapatkan data respons resistensi isolat bakteri tersebut terhadap campuran bahan aktif azoksistrobin dan difenokonazol. Penelitian terdiri atas lima tahapan, yaitu (1) pengambilan sampel pada lahan yang terdapat gejala busuk hitam; (2) isolasi bakteri menggunakan teknik penanaman jaringan; (3) seleksi isolat dengan uji sifat Gram, hipersensitif, hidrolisis pati, dan patogenesitas; (4) identifikasi menggunakan primer spesifik dan universal 16S rRNA; dan (5) analisis keanekaragaman genotipe dengan metode RFLP in silico dan keragaman fenotipe dengan mengukur bobot eksopolisakarida. Hasil karakterisasi dan identifikasi secara molekuler diperoleh lima isolat bakteri X. campestris pv. campestris, yaitu CLT01, CDA08, SDA02, SDA22, dan SDA26. Hasil analisis keanekaragaman genotipe menunjukkan bahwa kelima isolat memiliki keanekaragaman berdasarkan pemotongan fragmen DNA gen 16S rRNA, sedangkan keragaman fenotipe ditunjukkan dengan nilai inhibition consentration (IC) yang berbeda-beda. Isolat SDA22 memiliki nilai IC50 paling tinggi dan keanekaragaman secara genetik yang berbeda dibandingkan dengan isolat X. campestris pv. campestris lainnya. Penggunaan pestitida sintetis azoksistrobin dan difenokonazol secara terus-menerus dalam jangka waktu lama dikhawatirkan berdampak pada patogen seperti X. campestris pv. campestris. Oleh karena itu, alternatif pengendalian yang lain diperlukan agar tidak terjadi resistensi pada X. campestris pv. campestris.
{"title":"Character Diversity of Black Rot Bacterial Strains (Xanthomonas campestris pv. campestris) on Cabbage against Mixture of Active Ingredients Azoxystrobin and Diphenoconazole","authors":"Af’idzatuttama, A. A. Nawangsih, Giyanto","doi":"10.14692/jfi.19.2.45-56","DOIUrl":"https://doi.org/10.14692/jfi.19.2.45-56","url":null,"abstract":"Xanthomonas campestris pv. campestris merupakan bakteri penyebab penyakit busuk hitam (black rot) pada kubis. Petani masih mengandalkan pestitida sintetis azoksistrobin dan difenokonazol untuk mengendalikan penyakit pada tanaman kubis yang dikhawatirkan akan berdampak pada keanekaragaman X. campestris pv. campestris. Tujuan penelitian ialah mendapatkan data keanekaragaman secara genetik dan fenotipik isolat bakteri X. campestris pv. campestris, serta mendapatkan data respons resistensi isolat bakteri tersebut terhadap campuran bahan aktif azoksistrobin dan difenokonazol. Penelitian terdiri atas lima tahapan, yaitu (1) pengambilan sampel pada lahan yang terdapat gejala busuk hitam; (2) isolasi bakteri menggunakan teknik penanaman jaringan; (3) seleksi isolat dengan uji sifat Gram, hipersensitif, hidrolisis pati, dan patogenesitas; (4) identifikasi menggunakan primer spesifik dan universal 16S rRNA; dan (5) analisis keanekaragaman genotipe dengan metode RFLP in silico dan keragaman fenotipe dengan mengukur bobot eksopolisakarida. Hasil karakterisasi dan identifikasi secara molekuler diperoleh lima isolat bakteri X. campestris pv. campestris, yaitu CLT01, CDA08, SDA02, SDA22, dan SDA26. Hasil analisis keanekaragaman genotipe menunjukkan bahwa kelima isolat memiliki keanekaragaman berdasarkan pemotongan fragmen DNA gen 16S rRNA, sedangkan keragaman fenotipe ditunjukkan dengan nilai inhibition consentration (IC) yang berbeda-beda. Isolat SDA22 memiliki nilai IC50 paling tinggi dan keanekaragaman secara genetik yang berbeda dibandingkan dengan isolat X. campestris pv. campestris lainnya. Penggunaan pestitida sintetis azoksistrobin dan difenokonazol secara terus-menerus dalam jangka waktu lama dikhawatirkan berdampak pada patogen seperti X. campestris pv. campestris. Oleh karena itu, alternatif pengendalian yang lain diperlukan agar tidak terjadi resistensi pada X. campestris pv. campestris.","PeriodicalId":31619,"journal":{"name":"Jurnal Fitopatologi Indonesia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42046601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Population of Soil Nematodes in The Treatment of Brassicaceae Plant Waste Brassicaceae plants are known to contain glucosinolates which can be hydrolyzed into biofumigant compounds. This research was conducted to examine the effect of biofumigant derived from Brassicaceae plants waste on soil nematode population dynamics. Greenhouse experiment was carried out on 4 types of Brassicaceae plants waste, consisting of leaf waste of radish (Raphanus sativus), broccoli (Brassica oleracea var. italica), cabbage (Brassica oleracea var. capitata), and whole parts kamanilan weed (Roripa indica) in polybags containing 5 L of nematode infested soil. Plant waste was chopped ± 1 cm in size, then as much as 117 g per polybag was mixed in nematode infested soil, doused with water until wet and tightly closed for the biofumigation process for 14 days. At the end of biofumigation step, the polybags were opened for 3-5 days, then 7 day - old cucumber seedlings of var. Roberto 92 were planted and the plants were maintained in greenhouse for up to 8 weeks. Observation of the type and population of soil nematodes was carried out three times, i.e. before treatment, after treatment, and 8 weeks after planting. This experiment was designed in a completely randomized design with 6 treatments and 5 replications. The results of the study showed that the population of free-living nematodes (bacteriovorous nematodes and fungivorous nematodes) was increased in all treatment of Brassicaceae plants waste. On the other hand, there was a significant decrease in the phytonematodes population in the treatment of broccoli leaf waste. All Brassicaceae wastes was able to suppress population of Helicotylenchus sp., Rotylenchulus sp., and Xiphinema sp., significantly. Leaf waste of broccoli leaves showed the highest inhibition value against all phytonematodes genera with inhibition reached 100%.
{"title":"Populasi Nematoda Tanah pada Perlakuan Limbah Tanaman Brassicaceae","authors":"A. Ibrahim, Supramana, Giyanto","doi":"10.14692/jfi.19.1.19-29","DOIUrl":"https://doi.org/10.14692/jfi.19.1.19-29","url":null,"abstract":"Population of Soil Nematodes in The Treatment of Brassicaceae Plant Waste Brassicaceae plants are known to contain glucosinolates which can be hydrolyzed into biofumigant compounds. This research was conducted to examine the effect of biofumigant derived from Brassicaceae plants waste on soil nematode population dynamics. Greenhouse experiment was carried out on 4 types of Brassicaceae plants waste, consisting of leaf waste of radish (Raphanus sativus), broccoli (Brassica oleracea var. italica), cabbage (Brassica oleracea var. capitata), and whole parts kamanilan weed (Roripa indica) in polybags containing 5 L of nematode infested soil. Plant waste was chopped ± 1 cm in size, then as much as 117 g per polybag was mixed in nematode infested soil, doused with water until wet and tightly closed for the biofumigation process for 14 days. At the end of biofumigation step, the polybags were opened for 3-5 days, then 7 day - old cucumber seedlings of var. Roberto 92 were planted and the plants were maintained in greenhouse for up to 8 weeks. Observation of the type and population of soil nematodes was carried out three times, i.e. before treatment, after treatment, and 8 weeks after planting. This experiment was designed in a completely randomized design with 6 treatments and 5 replications. The results of the study showed that the population of free-living nematodes (bacteriovorous nematodes and fungivorous nematodes) was increased in all treatment of Brassicaceae plants waste. On the other hand, there was a significant decrease in the phytonematodes population in the treatment of broccoli leaf waste. All Brassicaceae wastes was able to suppress population of Helicotylenchus sp., Rotylenchulus sp., and Xiphinema sp., significantly. Leaf waste of broccoli leaves showed the highest inhibition value against all phytonematodes genera with inhibition reached 100%.","PeriodicalId":31619,"journal":{"name":"Jurnal Fitopatologi Indonesia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46437333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Suryo Wiyono, A. S. Suryaningsih, Astika Widhi Pratiwi
Effectiveness of Various PGPR Lineage in Controlling White Rust Diseases and Growth Promotion of Chrysanthemum White rust disease caused by Puccinia horiana is the most destructive disease of Chrysanthemum in Indonesia. There was no available effective control measure against the disease in Indonesia. This research aimed to examine the potency of five strains of plant growth-promoting rhizobacteria (PGPR) for controlling white rust disease on Chrysanthemum under field conditions. Five PGPR strains (Pseudomonas diminuta P14, Bacillus firmus J8, Lysinibacillus fusiformis C71, AKBR, AKS) and one commercially formulated PGPR were used in this study. The research was carried out in farmer fields with a randomized complete block experimental design. PGPR was applied by plant watering using bacterial suspension. All PGPR strains were effective in suppressing the white rust disease incidence and its severity. The most effective PGPR strain to suppress white rust disease was Bacillus firmus J8 and the most effective strain to increase the growth of Chrysanthemum plants was Lysinibacillus fusiformis C71. The five PGPR strains tested and commercial formulation increased the diameter of the flower.
{"title":"Keefektifan Galur PGPR dalam Pengendalian Penyakit Karat Putih dan Peningkatan Pertumbuhan Tanaman Krisan","authors":"Suryo Wiyono, A. S. Suryaningsih, Astika Widhi Pratiwi","doi":"10.14692/jfi.19.1.11-18","DOIUrl":"https://doi.org/10.14692/jfi.19.1.11-18","url":null,"abstract":"Effectiveness of Various PGPR Lineage in Controlling White Rust Diseases and Growth Promotion of Chrysanthemum White rust disease caused by Puccinia horiana is the most destructive disease of Chrysanthemum in Indonesia. There was no available effective control measure against the disease in Indonesia. This research aimed to examine the potency of five strains of plant growth-promoting rhizobacteria (PGPR) for controlling white rust disease on Chrysanthemum under field conditions. Five PGPR strains (Pseudomonas diminuta P14, Bacillus firmus J8, Lysinibacillus fusiformis C71, AKBR, AKS) and one commercially formulated PGPR were used in this study. The research was carried out in farmer fields with a randomized complete block experimental design. PGPR was applied by plant watering using bacterial suspension. All PGPR strains were effective in suppressing the white rust disease incidence and its severity. The most effective PGPR strain to suppress white rust disease was Bacillus firmus J8 and the most effective strain to increase the growth of Chrysanthemum plants was Lysinibacillus fusiformis C71. The five PGPR strains tested and commercial formulation increased the diameter of the flower.","PeriodicalId":31619,"journal":{"name":"Jurnal Fitopatologi Indonesia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43373603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This editorial contains the front cover, editorial page, and back cover of the Jurnal Fitopatologi Indonesia Vol. 19 No. 1, January 2023
本社论包含2023年1月印度尼西亚《fitopatologii》第19卷第1期的封面、社论版和封底
{"title":"Cover Jurnal Fitopatologi Vol. 19 No. 1, January 2023","authors":"Editor Jurnal Fitopatologi Indonesia","doi":"10.14692/jfi.19.1.i","DOIUrl":"https://doi.org/10.14692/jfi.19.1.i","url":null,"abstract":"This editorial contains the front cover, editorial page, and back cover of the Jurnal Fitopatologi Indonesia Vol. 19 No. 1, January 2023","PeriodicalId":31619,"journal":{"name":"Jurnal Fitopatologi Indonesia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46414975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Infrared Thermography for Early Detection of Pepper yellow leaf curl virus on Chili Plants Observations of plant pests and diseases are generally carried out by looking for visual symptoms for each disease target. Agricultural technology 4.0 began to be used for the development of plant disease detection methods. It was reported that there were differences in color and temperature between diseased and healthy plants which could be recorded by a thermal camera. This study aimed to determine the potential of the FLIR One Pro-IOS thermal camera to record differences in color and temperature between viral-infected and healthy chili plants. Chili plants in the greenhouse that were inoculated with Pepper yellow leaf curl virus (PYLCV) experienced an increase in temperature 3 days after inoculation (28.62 ℃) compared to plants that were not inoculated with PYLCV (27.32 ℃). Digital image recording of chili leaf samples from the field showed that plants infected by multiple viruses (Chilli veinal mottle virus, Pepper mottle virus and PYLCV) has higher temperature than those infected with a single virus. The lowest and highest mean temperatures were recorded in plant samples infected with PepMV (17.74 ℃) and mixed infected by PYLCV and ChiVMV (25.68 ℃). Digital images of virus-infected plants tend to show a predominance of bright yellow, while virus-free plants showed a predominance of dark purple. Further analysis confirmed higher digital numbers for diseased plant images than healthy plants. The thermography method has the potential to be an early detection method because it can detect viral infection before visual symptoms appear.
辣椒黄曲叶病毒早期检测的红外热像仪对植物病虫害的观察通常是通过寻找每个疾病目标的视觉症状来进行的。农业技术4.0开始被用于植物病害检测方法的开发。据报道，患病植物和健康植物在颜色和温度上存在差异，这可以通过热像仪记录下来。这项研究旨在确定FLIR One Pro IOS热像仪记录病毒感染和健康辣椒植物之间颜色和温度差异的潜力。与未接种PYLCV的辣椒（27.32℃）相比，接种PYLC病毒的辣椒在接种后第3天（28.62℃）的温度升高。对田间辣椒叶样本的数字图像记录显示，被多种病毒（辣椒脉斑驳病毒、辣椒斑驳病毒和PYLCV）感染的植物比被单一病毒感染的植物温度更高。感染PepMV（17.74℃）和混合感染PYLCV和ChiVMV（25.68℃）的植物样品的平均温度最低和最高。感染病毒的植物的数字图像往往以亮黄色为主，而无病毒的植物则以深紫色为主。进一步的分析证实，患病植物图像的数字数字高于健康植物。热成像方法有可能成为一种早期检测方法，因为它可以在视觉症状出现之前检测病毒感染。
{"title":"Metode Termografi Inframerah untuk Deteksi Dini Pepper yellow leaf curl virus pada Tanaman Cabai","authors":"Triyani Dumaria, S. H. Hidayat, P. Hidayat","doi":"10.14692/jfi.19.1.1-10","DOIUrl":"https://doi.org/10.14692/jfi.19.1.1-10","url":null,"abstract":"Infrared Thermography for Early Detection of Pepper yellow leaf curl virus on Chili Plants Observations of plant pests and diseases are generally carried out by looking for visual symptoms for each disease target. Agricultural technology 4.0 began to be used for the development of plant disease detection methods. It was reported that there were differences in color and temperature between diseased and healthy plants which could be recorded by a thermal camera. This study aimed to determine the potential of the FLIR One Pro-IOS thermal camera to record differences in color and temperature between viral-infected and healthy chili plants. Chili plants in the greenhouse that were inoculated with Pepper yellow leaf curl virus (PYLCV) experienced an increase in temperature 3 days after inoculation (28.62 ℃) compared to plants that were not inoculated with PYLCV (27.32 ℃). Digital image recording of chili leaf samples from the field showed that plants infected by multiple viruses (Chilli veinal mottle virus, Pepper mottle virus and PYLCV) has higher temperature than those infected with a single virus. The lowest and highest mean temperatures were recorded in plant samples infected with PepMV (17.74 ℃) and mixed infected by PYLCV and ChiVMV (25.68 ℃). Digital images of virus-infected plants tend to show a predominance of bright yellow, while virus-free plants showed a predominance of dark purple. Further analysis confirmed higher digital numbers for diseased plant images than healthy plants. The thermography method has the potential to be an early detection method because it can detect viral infection before visual symptoms appear.","PeriodicalId":31619,"journal":{"name":"Jurnal Fitopatologi Indonesia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42921593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Plant Parasitic Nematode of Strawberry in Ciwidey-West Java Strawberry is a horticultural crop that is widely cultivated in the highlands. One of the obstacles in strawberry production is plant parasitic nematode infection. Ciwidey District is one of the strawberry production centers in Bandung Regency, West Java Province. Several strawberry farms in Ciwidey showed symptoms of nematode infection, which affect the roots and crowns of the plants. There is limited reports of plant parasitic nematodes on strawberry plants in Indonesia , therefore this study was initiated to identify the genera of nematodes associated with strawberries and measure their abundance. Plant samples were taken by a purposive sampling method on symptomatic plants. Soil samples were extracted by centrifugation floatation method, root samples by fogging method; while leaf samples were cut into pieces, soaked in a cup, then incubated in the refrigerator for 24 hours. Nematodes were identified and their abundance was counted. Symptoms of plant parasitic nematode on the strawberry plant canopy involved stunted growth, reddened leaves, curled or wrinkled small leaves, and chlorosis. Symptoms on the roots are in the form of root lesions, shortened roots, reduced number of roots, swelling at the tip of the root, and root galls. Six species of nematodes were identified from all samples, i.e. Aphelenchoides besseyi, A. bicaudatus, Meloidogyne spp., Pratylenchus sp., Scutellonema sp., and Tylenchus sp. The abundance of nematodes found varied between 1–42 nematodes per 100 mL-1 soil and 2–29 nematodes per 5 g root-1. Among the nematodes found, Scutellonema was the first nematode reported to be associated with strawberry plants in Indonesia.
{"title":"Nematoda Parasit Tumbuhan pada Tanaman Stroberi di Ciwidey-Jawa Barat","authors":"F. Kurniawati, D. Anindita, Supramana","doi":"10.14692/jfi.19.1.30-38","DOIUrl":"https://doi.org/10.14692/jfi.19.1.30-38","url":null,"abstract":"Plant Parasitic Nematode of Strawberry in Ciwidey-West Java Strawberry is a horticultural crop that is widely cultivated in the highlands. One of the obstacles in strawberry production is plant parasitic nematode infection. Ciwidey District is one of the strawberry production centers in Bandung Regency, West Java Province. Several strawberry farms in Ciwidey showed symptoms of nematode infection, which affect the roots and crowns of the plants. There is limited reports of plant parasitic nematodes on strawberry plants in Indonesia , therefore this study was initiated to identify the genera of nematodes associated with strawberries and measure their abundance. Plant samples were taken by a purposive sampling method on symptomatic plants. Soil samples were extracted by centrifugation floatation method, root samples by fogging method; while leaf samples were cut into pieces, soaked in a cup, then incubated in the refrigerator for 24 hours. Nematodes were identified and their abundance was counted. Symptoms of plant parasitic nematode on the strawberry plant canopy involved stunted growth, reddened leaves, curled or wrinkled small leaves, and chlorosis. Symptoms on the roots are in the form of root lesions, shortened roots, reduced number of roots, swelling at the tip of the root, and root galls. Six species of nematodes were identified from all samples, i.e. Aphelenchoides besseyi, A. bicaudatus, Meloidogyne spp., Pratylenchus sp., Scutellonema sp., and Tylenchus sp. The abundance of nematodes found varied between 1–42 nematodes per 100 mL-1 soil and 2–29 nematodes per 5 g root-1. Among the nematodes found, Scutellonema was the first nematode reported to be associated with strawberry plants in Indonesia.","PeriodicalId":31619,"journal":{"name":"Jurnal Fitopatologi Indonesia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49286587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Infection of Ageratum yellow vein virus on Weed Crassocephalum crepidioides in Bengkulu Weeds with symptoms of virus infection were found on three cultivation areas of papaya (Carica papaya) in Bengkulu province, Indonesia. Symptoms on weeds involved yellowing of lamina and vein, yellow mosaic, and leaf curling. This study aimed to identify and detect Begomovirus on weed species, Crassocephalum crepidioides. Virus detection was conducted by polymerase chain reaction method using a pair of universal primers for Begomovirus (SPG1/SPG2). A DNA fragment of 912 bp in size was successfully amplified from C. crepidioides with leaf curl symptom. Analysis using Blastn showed that Begomovirus sample from C. crepidioides has the closest relationship with Ageratum yellow vein virus (AYVV) isolate from Taiwan (DQ866134.1) with nucleotide homology of 99%. This is the first report of AYVV infection in C. crepidioides in Indonesia.
{"title":"Infeksi Ageratum yellow vein virus pada Gulma Crassocephalum crepidioides di Bengkulu","authors":"Nia Kurniati Br. Marpaung, Mimi Sutrawati, Dwi Wahyuni Ganefianti, Ridha Rizki Novanda, Tunjung Pamekas","doi":"10.14692/jfi.19.1.39-44","DOIUrl":"https://doi.org/10.14692/jfi.19.1.39-44","url":null,"abstract":"Infection of Ageratum yellow vein virus on Weed Crassocephalum crepidioides in Bengkulu Weeds with symptoms of virus infection were found on three cultivation areas of papaya (Carica papaya) in Bengkulu province, Indonesia. Symptoms on weeds involved yellowing of lamina and vein, yellow mosaic, and leaf curling. This study aimed to identify and detect Begomovirus on weed species, Crassocephalum crepidioides. Virus detection was conducted by polymerase chain reaction method using a pair of universal primers for Begomovirus (SPG1/SPG2). A DNA fragment of 912 bp in size was successfully amplified from C. crepidioides with leaf curl symptom. Analysis using Blastn showed that Begomovirus sample from C. crepidioides has the closest relationship with Ageratum yellow vein virus (AYVV) isolate from Taiwan (DQ866134.1) with nucleotide homology of 99%. This is the first report of AYVV infection in C. crepidioides in Indonesia.","PeriodicalId":31619,"journal":{"name":"Jurnal Fitopatologi Indonesia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47898799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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