Food and Chemical Toxicology最新文献

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Update to RIFM fragrance ingredient safety assessment, cyclohexanepropanol, α-ethyl-2,2,6-trimethyl-, CAS Registry Number 60241-52-3

IF 3.9 3区医学 Q2 FOOD SCIENCE & TECHNOLOGY

Food and Chemical Toxicology

Pub Date : 2025-02-14 DOI: 10.1016/j.fct.2025.115323

A.M. Api , A. Bartlett , D. Belsito , D. Botelho , M. Bruze , A. Bryant-Friedrich , G.A. Burton Jr. , M.A. Cancellieri , H. Chon , M. Cronin , S. Crotty , M.L. Dagli , W. Dekant , C. Deodhar , K. Farrell , A.D. Fryer , L. Jones , K. Joshi , A. Lapczynski , D.L. Laskin , Y. Thakkar

引用次数: 0

RIFM fragrance ingredient safety assessment, (1aα,4α,7aα)-1a,3,3,4,6,6-hexamethyl-1a,2,3,4,5,6,7,7a-octahydronapth[3,3-b]oxirene, CAS Registry Number 94400-98-3

IF 3.9 3区医学 Q2 FOOD SCIENCE & TECHNOLOGY

Food and Chemical Toxicology

Pub Date : 2025-02-13 DOI: 10.1016/j.fct.2025.115321

A.M. Api , A. Bartlett , D. Belsito , D. Botelho , M. Bruze , A. Bryant-Friedrich , G.A. Burton Jr. , M.A. Cancellieri , H. Chon , M.L. Dagli , W. Dekant , C. Deodhar , K. Farrell , A.D. Fryer , L. Jones , K. Joshi , A. Lapczynski , M. Lavelle , I. Lee , H. Moustakas , Y. Tokura

引用次数: 0

Melatonin protects against cadmium-induced endoplasmic reticulum stress and ferroptosis through activating Nrf2/HO-1 signaling pathway in mice lung

IF 3.9 3区医学 Q2 FOOD SCIENCE & TECHNOLOGY

Food and Chemical Toxicology

Pub Date : 2025-02-13 DOI: 10.1016/j.fct.2025.115324

Ziyang Huang , Ruijia Xu , Zhongjun Wan , Chao Liu , Jinquan Li , Jun He , Li Li

Cadmium (Cd) is a prevalent heavy metal pollutant known to cause lung damage. However, the mechanisms underlying Cd-induced lung injury and the associated therapeutic strategies remain unclear. By establishing Cd-induced lung damage models both in vivo and in vitro, we observed that Cd inhibited the Nrf2/HO-1 signaling pathway, disrupted the redox balance in lung tissue, accelerated endoplasmic reticulum (ER) stress, and promoted ferroptosis, ultimately leading to lung injury. Melatonin (Mel), a potent reactive oxygen species (ROS) inhibitor with high antioxidative efficacy, mitigated the increasing in ROS and the decreasing in superoxide dismutase levels induced by Cd, as well as the upregulation of PERK-eIF2α-ATF4 signaling associated with ER stress, through the activation of the Nrf2/HO-1 signaling pathway. Furthermore, Mel administration not only prevented Cd-induced iron overload but also reduced lipid peroxidation levels, thereby improving mitochondrial morphological alterations. Collectively, our results demonstrated that Mel treatment alleviated Cd-induced lung injury by inhibiting oxidative stress, which in turn ameliorated ER stress and ferroptosis through the activation of the Nrf2/HO-1 pathway.

镉（Cd）是一种普遍存在的重金属污染物，已知会造成肺损伤。然而，镉诱导肺损伤的机制及相关治疗策略仍不清楚。通过在体内和体外建立镉诱导的肺损伤模型，我们观察到镉抑制了Nrf2/HO-1信号通路，破坏了肺组织中的氧化还原平衡，加速了内质网（ER）应激，促进了铁变态反应，最终导致肺损伤。褪黑素（Melatonin，Mel）是一种强效活性氧（ROS）抑制剂，具有很高的抗氧化功效，它通过激活Nrf2/HO-1信号通路，缓解了镉诱导的ROS增加和超氧化物歧化酶水平下降，以及与ER应激相关的PERK-eIF2α-ATF4信号的上调。此外，服用 Mel 不仅能防止镉诱导的铁超载，还能降低脂质过氧化水平，从而改善线粒体形态学改变。总之，我们的研究结果表明，Mel治疗通过抑制氧化应激减轻了镉诱导的肺损伤，而氧化应激又通过激活Nrf2/HO-1通路改善了ER应激和铁变态反应。

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引用次数: 0

RIFM fragrance ingredient safety assessment, cyclododecyl formate, CAS Registry Number 59052-82-3

IF 3.9 3区医学 Q2 FOOD SCIENCE & TECHNOLOGY

Food and Chemical Toxicology

Pub Date : 2025-02-12 DOI: 10.1016/j.fct.2025.115318

引用次数: 0

Coexposure to fluoride and sulfur dioxide aggravates enamel mineralization disorders in mice by disrupting calcium homeostasis-mediated endoplasmic reticulum stress

IF 3.9 3区医学 Q2 FOOD SCIENCE & TECHNOLOGY

Food and Chemical Toxicology

Pub Date : 2025-02-10 DOI: 10.1016/j.fct.2025.115317

Wentai Wang , Na Yang , Junlin Yang , Jiaojiao He , Guohui Bai , Chenglong Tu

Prevalence of fluoride and sulfur dioxide (SO₂) cocontamination in the environment poses a serious threat to various human organs, especially the teeth. However, direct evidence linking coexposure to fluoride and SO₂ with enamel mineralization disorders is lacking. Here, we investigated the mechanisms through which fluoride and SO₂ exposure, either alone or in combination, affects enamel mineralization in mouse and LS8 cell models. Coexposure to fluoride and SO₂ resulted in more severe enamel mineralization disorders compared with those in the control or individual exposure groups. The coexposure caused significant pathological changes and retention of enamel matrix. Furthermore, the coexposure upregulated the expression of membrane calcium channels (Cav1.2), calmodulin-dependent protein kinase II (CaMKII), endoplasmic reticulum calcium ion(Ca²⁺)-release channel (IP₃R), and endoplasmic reticulum stress (ERS) marker protein (GRP78), and significantly downregulated the expression of endoplasmic reticulum (ER) Ca²⁺-uptake pump protein (SERCA2) and calreticulin (CRT). Investigations using Amlodipine (Am), Tunicamycin (Tm) and CDN1163 revealed that the coexposure exacerbated enamel mineralization disorders by disrupting calcium homeostasis and subsequently triggering ERS. Overall, this study highlights that coexposure to fluoride and SO₂ affects ER Ca²⁺ content through cytoplasmic calcium overload, triggers ERS, and increases the risk of enamel mineralization disorders. Activation of ERS, induced by disruption of calcium homeostasis, may play a key role in fluoride and SO₂-induced enamel mineralization disorders. The insights obtained from this study should be valuable for devising strategies to mitigate the effects of fluoride and SO₂ coexposure on enamel mineralization disorders.

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引用次数: 0

Resolving complexity: Identification of altersetin and toxin mixtures responsible for the immunomodulatory, antiestrogenic and genotoxic potential of a complex Alternaria mycotoxin extract

IF 3.9 3区医学 Q2 FOOD SCIENCE & TECHNOLOGY

Food and Chemical Toxicology

Pub Date : 2025-02-09 DOI: 10.1016/j.fct.2025.115315

Vanessa Partsch , Francesco Crudo , Daniel Piller , Elisabeth Varga , Giorgia Del Favero , Doris Marko

Alternaria mycotoxins may pose significant risks to human health due to their diverse spectrum of adverse effects and frequent occurrences in food. A previous study demonstrated the immunosuppressive, antiestrogenic, and genotoxic potential of a complex Alternaria mycotoxin extract (CE). The present study aimed to elucidate specific Alternaria mycotoxins or combinations thereof responsible for toxicity.

Following toxicity-guided fractionation of the CE, a multiparametric panel of assays was applied to assess different endpoints. These included immunomodulatory effects (NF-κB reporter gene assay in THP1-Lucia™ monocytes), estrogenicity/antiestrogenicity (alkaline phosphatase assay in Ishikawa cells) and genotoxicity (γH2AX and alkaline comet assays in HepG2 cells).

LC-MS/MS analysis revealed prominent mycotoxins in the active fractions, with altersetin (AST) identified as a novel key compound exhibiting immunoinhibitory (≥2 μM) and antiestrogenic (≥5 μM) properties in vitro. Additionally, while specific mycotoxin combinations explained the toxicity of active fractions, some effects remained unexplained, suggesting the presence of unidentified bioactive substances.

This study underscores the significance of AST and specific toxin mixtures as major contributors to CE toxicity. Further, it highlights the importance of considering combinatory effects in risk assessment of Alternaria mycotoxins as well as further investigation of unknown Alternaria metabolites, which may pose additional health risks.

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引用次数: 0

Developmental and cardiotoxic effects of cyhalofop-butyl in zebrafish embryos

IF 3.9 3区医学 Q2 FOOD SCIENCE & TECHNOLOGY

Food and Chemical Toxicology

Pub Date : 2025-02-08 DOI: 10.1016/j.fct.2025.115316

Bo Peng, Xinyi Zhu, Li Geng, Wenping Xu, Jiagao Cheng, Liming Tao, Yang Zhang

This study evaluates the developmental and cardiotoxic effects of cyhalofop-butyl, a commonly used herbicide in rice agriculture, on zebrafish (Danio rerio) embryos. Despite its widespread application, the risk assessment of cyhalofop-butyl for aquatic organisms, especially fish, is still lacking. Focusing on the cardiac system, we used a zebrafish model to evaluate developmental abnormalities, changes in cardiac morphology and function, markers of oxidative stress, and altered gene expression. The results suggest that cyhalofop-butyl induces oxidative stress, lipid accumulation, and apoptosis in zebrafish embryos. In addition, it can lead to abnormal embryonic development and cardiac morphological dysfunction (such as pericardial edema, decreased heart rate, and red blood cell (RBC) flow rate, and cardiac linearization). Cyhalofop-butyl also significantly alters the expression of cardiac-related genes, including myl7, vmhc, myh6, nkx2.5, tbx5, nppa, has2, and myh7. In summary, cyhalofop-butyl elicits both dysplasia and cardiotoxicity in zebrafish embryos, highlighting the need for further safety risk evaluation of this herbicide in aquatic ecosystems.

本研究评估了氰氟草酯这种常用于水稻种植的除草剂对斑马鱼（Danio rerio）胚胎发育和心脏毒性的影响。尽管氰氟草酯被广泛使用，但对水生生物（尤其是鱼类）的风险评估仍然缺乏。以心脏系统为重点，我们使用斑马鱼模型来评估发育异常、心脏形态和功能变化、氧化应激标记以及基因表达的改变。结果表明，氰氟草酯会诱发斑马鱼胚胎氧化应激、脂质积累和细胞凋亡。此外，氰氟草酯还会导致胚胎发育异常和心脏形态功能障碍（如心包水肿、心率和红细胞（RBC）流速下降以及心脏线性化）。氰氟草酯还会显著改变心脏相关基因的表达，包括 myl7、vmhc、myh6、nkx2.5、tbx5、nppa、has2 和 myh7。总之，氰氟草酯会引起斑马鱼胚胎发育不良和心脏毒性，因此有必要进一步评估这种除草剂在水生生态系统中的安全风险。

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引用次数: 0

Environmental contamination by bisphenols: From plastic production to modulation of the intestinal morphophysiology in experimental models

IF 3.9 3区医学 Q2 FOOD SCIENCE & TECHNOLOGY

Food and Chemical Toxicology

Pub Date : 2025-02-07 DOI: 10.1016/j.fct.2025.115280

Beatriz Gouvêa de Luca , Patricia Pereira Almeida , Reinaldo Röpke Junior , Débora Júlia Silva Soares , Eliete Dalla Corte Frantz , Leandro Miranda-Alves , Milena Barcza Stockler-Pinto , Clarice Machado dos Santos , D'Angelo Carlo Magliano

Bisphenols are frequently found in a range of plastic products and have been associated with the development of diseases such as diabetes mellitus type 2 and obesity. These compounds are known as endocrine disruptors and have led to restrictions on their use due to their presence in the environment and their association with non-communicable chronic diseases. The gastrointestinal tract, being the primary site of food and water absorption, is particularly vulnerable to the effects of bisphenols. For this reason, a review of studies showing associations between bisphenols exposure and adverse effects in the gut microbiota, morphology tissue, gut permeability, and on the enteric nervous system was carried out. We have included perinatal studies and in different adult experimental models. The effects of bisphenol exposure on the gut microbiota are complex and varied. Bisphenol exposure generally leads to a decrease in microbial diversity and may impact the integrity of the intestinal barrier, resulting in elevated levels of inflammation, changes in morphological and metabolic characteristics of the gut, modifications in tight junction expression, and changes in goblet cell expression. In addition, bisphenol exposure in the perinatal phase can lead to important intestinal changes, including increased colonic inflammation and decreased colonic paracellular permeability.

{"title":"Environmental contamination by bisphenols: From plastic production to modulation of the intestinal morphophysiology in experimental models","authors":"Beatriz Gouvêa de Luca , Patricia Pereira Almeida , Reinaldo Röpke Junior , Débora Júlia Silva Soares , Eliete Dalla Corte Frantz , Leandro Miranda-Alves , Milena Barcza Stockler-Pinto , Clarice Machado dos Santos , D'Angelo Carlo Magliano","doi":"10.1016/j.fct.2025.115280","DOIUrl":"10.1016/j.fct.2025.115280","url":null,"abstract":"<div><div>Bisphenols are frequently found in a range of plastic products and have been associated with the development of diseases such as diabetes mellitus type 2 and obesity. These compounds are known as endocrine disruptors and have led to restrictions on their use due to their presence in the environment and their association with non-communicable chronic diseases. The gastrointestinal tract, being the primary site of food and water absorption, is particularly vulnerable to the effects of bisphenols. For this reason, a review of studies showing associations between bisphenols exposure and adverse effects in the gut microbiota, morphology tissue, gut permeability, and on the enteric nervous system was carried out. We have included perinatal studies and in different adult experimental models. The effects of bisphenol exposure on the gut microbiota are complex and varied. Bisphenol exposure generally leads to a decrease in microbial diversity and may impact the integrity of the intestinal barrier, resulting in elevated levels of inflammation, changes in morphological and metabolic characteristics of the gut, modifications in tight junction expression, and changes in goblet cell expression. In addition, bisphenol exposure in the perinatal phase can lead to important intestinal changes, including increased colonic inflammation and decreased colonic paracellular permeability.</div></div>","PeriodicalId":317,"journal":{"name":"Food and Chemical Toxicology","volume":"197 ","pages":"Article 115280"},"PeriodicalIF":3.9,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143381268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Safety evaluation of Limosilactobacillus fermentum PS150 for use as a commercial psychobiotic

IF 3.9 3区医学 Q2 FOOD SCIENCE & TECHNOLOGY

Food and Chemical Toxicology

Pub Date : 2025-02-07 DOI: 10.1016/j.fct.2025.115312

Li-Hao Cheng , Chien-Chen Wu , Chin-Lin Huang , Yu-Hsuan Wei , Pei-Jun Wen , Shih-Hau Chiu , Chien-Chi Chen , Ching-Ting Lin , Po-Lin Liao

The psychobiotic Limosilactobacillus fermentum PS150 (PS150), isolated from fermented meat sausage, has antidepressant, anxiolytic, and sleep-improving properties. This study investigated the safety of PS150 using a genome-based safety evaluation, antibiotic resistance profiles, mutagenicity, clastogenicity, 28-day subacute toxicity, and gastrointestinal tolerance. Bioinformatics analysis indicated that PS150 did not carry genes encoding antimicrobial resistance, virulence factors, or enzymes related to biogenic amine production. Additionally, PS150 was sensitive to the eight antibiotics tested. Ames test results showed no signs of increased reverse mutations following PS150 treatment. Further, PS150 treatment did not increase the frequency of chromosomal aberrations or number of micronuclei, and administration of PS150 (1.3 × 10¹¹ CFU/kg, 2.6 × 10¹¹ CFU/kg and 4.3 × 10¹¹ CFU/kg) for 28 days did not cause any toxicity or mortality in mice. PS150 exhibited superior gastrointestinal tolerance both in vitro and in vivo, enabling it to endure and survive the digestive processes. In conclusion, our results suggest that L. fermentum PS150 is safe in mice, supporting its potential as a psychobiotic candidate for human use. The 28-day “No Observed Adverse Effect Level (NOAEL)” is defined at the highest dose of 4.3 × 10¹¹ CFU/kg body weight/day for the PS150 powder under the test conditions employed.

{"title":"Safety evaluation of Limosilactobacillus fermentum PS150 for use as a commercial psychobiotic","authors":"Li-Hao Cheng , Chien-Chen Wu , Chin-Lin Huang , Yu-Hsuan Wei , Pei-Jun Wen , Shih-Hau Chiu , Chien-Chi Chen , Ching-Ting Lin , Po-Lin Liao","doi":"10.1016/j.fct.2025.115312","DOIUrl":"10.1016/j.fct.2025.115312","url":null,"abstract":"<div><div>The psychobiotic <em>Limosilactobacillus fermentum</em> PS150 (PS150), isolated from fermented meat sausage, has antidepressant, anxiolytic, and sleep-improving properties. This study investigated the safety of PS150 using a genome-based safety evaluation, antibiotic resistance profiles, mutagenicity, clastogenicity, 28-day subacute toxicity, and gastrointestinal tolerance. Bioinformatics analysis indicated that PS150 did not carry genes encoding antimicrobial resistance, virulence factors, or enzymes related to biogenic amine production. Additionally, PS150 was sensitive to the eight antibiotics tested. Ames test results showed no signs of increased reverse mutations following PS150 treatment. Further, PS150 treatment did not increase the frequency of chromosomal aberrations or number of micronuclei, and administration of PS150 (1.3 × 10<sup>11</sup> CFU/kg, 2.6 × 10<sup>11</sup> CFU/kg and 4.3 × 10<sup>11</sup> CFU/kg) for 28 days did not cause any toxicity or mortality in mice. PS150 exhibited superior gastrointestinal tolerance both <em>in vitro</em> and <em>in vivo</em>, enabling it to endure and survive the digestive processes. In conclusion, our results suggest that <em>L. fermentum</em> PS150 is safe in mice, supporting its potential as a psychobiotic candidate for human use. The 28-day “No Observed Adverse Effect Level (NOAEL)” is defined at the highest dose of 4.3 × 10<sup>11</sup> CFU/kg body weight/day for the PS150 powder under the test conditions employed.</div></div>","PeriodicalId":317,"journal":{"name":"Food and Chemical Toxicology","volume":"197 ","pages":"Article 115312"},"PeriodicalIF":3.9,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143373632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fraglide-1 from traditional Chinese aromatic vinegar: A natural AhR antagonist for atopic dermatitis

IF 3.9 3区医学 Q2 FOOD SCIENCE & TECHNOLOGY

Food and Chemical Toxicology

Pub Date : 2025-02-07 DOI: 10.1016/j.fct.2025.115301

Kosuke Kato , Miki Akamatsu , Saya Kakimaru , Mayuko Koreishi , Masahiro Takagi , Masahiro Miyashita , Yoshiyuki Murata , Yoshimasa Nakamura , Ayano Satoh , Yoshio Tsujino

Traditional Chinese Zhenjiang aromatic vinegar (Kozu) contains Fraglide-1 (FG1), a bioactive lactone with demonstrated peroxisome proliferator-activated receptor gamma (PPARγ) agonist and antioxidant activities. This study explored FG1's novel ability to antagonize the aryl hydrocarbon receptor (AhR) signaling pathway, which regulates artemin expression and contributes to itching and inflammation in atopic dermatitis. Through molecular docking simulations and cell-based assays in human keratinocytes, we demonstrated FG1's potent antagonistic activity against AhR signaling. FG1 effectively suppressed FICZ-induced inflammatory responses, including artemin expression, with potency (half maximal inhibitory concentration, IC₅₀ = 5.1 μM) comparable to the synthetic antagonist StemRegenin 1 (SR1) while demonstrating a superior safety profile (median lethal concentration, LC₅₀ > 100 μM vs. 27.5 μM for SR1). These findings expand our understanding of bioactive compounds from traditional fermented foods and their regulatory effects on AhR signaling, providing a foundation for future studies on FG1's role in modulating skin inflammation.

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