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Template derivatization-mediated synthesis of highly ordered paper-based imprinted chip for enhanced sulfadiazine analysis 模板衍生化介导合成用于磺胺嘧啶分析的高阶纸基印迹芯片
IF 9.8 1区 农林科学 Q1 CHEMISTRY, APPLIED Pub Date : 2026-04-15 Epub Date: 2026-02-18 DOI: 10.1016/j.foodchem.2026.148493
Donghui Wang , Yuanyuan Liu , Ningning Li , Wei Liu , Yang Li , Yiming Zhang
Molecularly imprinted polymers (MIPs) have gained significant traction in food contaminant analysis owing to their tailorable binding sites and functionalities. Nevertheless, the analysis performance of MIPs fabricated via conventional imprinting methods has been hindered by heterogeneous binding cavities. Herein, we first present a template-derivatization-based oriented surface imprinting strategy to construct paper-based metal organic framework (MOF)-imprinted chips with highly ordered MIPs (FP@B-MOF@MIP) for enhanced sulfadiazine (SDZ) analysis. Cis-dihydroxy-derived SDZ (SDZ-CD) as the dummy template endowed imprinting cavities with high orientation via boronate-affinity oriented recognition. FP@B-MOF@MIP achieved good absorption capacity (76.30 mg g−1) and selectivity with the imprinting factor of 5.6 for SDZ. A colorimetric sensor based on FP@B-MOF@MIP was further successfully constructed for the visual determination of trace SDZ in food samples with a low detection limit of 0.0048 mg L−1. This work provides a flexible strategy to improve the performance of MIPs for monitoring trace contaminants in foodstuffs.
分子印迹聚合物(MIPs)由于其可定制的结合位点和功能,在食品污染物分析中获得了显著的吸引力。然而,通过传统的印迹方法制备的MIPs的分析性能受到非均匀结合腔的阻碍。在此,我们首先提出了一种基于模板衍生的定向表面印迹策略,以构建具有高度有序MIPs (FP@B-MOF@MIP)的纸基金属有机框架(MOF)印迹芯片,用于增强磺胺嘧啶(SDZ)分析。顺式二羟基衍生的SDZ (SDZ- cd)作为假模板通过硼离子亲和取向识别赋予印迹腔高取向。FP@B-MOF@MIP对SDZ具有良好的吸附量(76.30 mg g−1)和选择性,印迹因子为5.6。构建了基于FP@B-MOF@MIP的比色传感器,用于食品样品中痕量SDZ的视觉检测,检出限低至0.0048 mg L−1。这项工作提供了一种灵活的策略,以提高MIPs监测食品中痕量污染物的性能。
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引用次数: 0
Construction of lignin–metal polyphenol networks and their application in smart paper-based active packaging: Multifunctional materials with both antioxidant and UV-shielding functions 木质素-金属多酚网络的构建及其在智能纸质活性包装中的应用:兼具抗氧化和防紫外线功能的多功能材料。
IF 9.8 1区 农林科学 Q1 CHEMISTRY, APPLIED Pub Date : 2026-04-15 Epub Date: 2026-02-18 DOI: 10.1016/j.foodchem.2026.148498
Lirong Lu , Jiatian Zhu , Jingpeng Zhou , Xi Cheng , Baojie Liu , Chengrong Qin , Chen Liang , Caoxing Huang , Shuangquan Yao
Facing the urgent need for sustainable and functional food packaging, developing high-performance materials from natural polymers is crucial. Herein, we propose a green strategy inspired by crustacean exoskeletons to construct lignin–metal polyphenol networks (L-MPNs) via proton-based ionic liquid pretreatment and metal-ion coordination. This forms a stable 3D network utilizing lignin's phenolic hydroxyl groups, which significantly enhances antioxidant activity, achieves >99% UV shielding, and exhibits 92.5% DPPH radical scavenging capacity. Applied to paper packaging, Ferric-ion-coordinated lignin-metal polyphenol networks (FL-MPNs, 100 g/m2) impart excellent barrier and antioxidant properties alongside maintained mechanical strength, cutting water vapor transmission by ∼40%. In freshness tests, they reduced mass loss to 3.52% and pH change to 0.17, effectively inhibiting food deterioration. Thus, FL-MPNs advance lignin valorization for next-generation sustainable smart packaging to ensure safer, more stable food preservation.
面对可持续和功能性食品包装的迫切需求,从天然聚合物中开发高性能材料至关重要。在此,我们提出了一种受甲壳类动物外骨骼启发的绿色策略,通过质子基离子液体预处理和金属离子配位构建木质素-金属多酚网络(L-MPNs)。利用木质素的酚羟基形成稳定的三维网络,显著增强了抗氧化活性,达到bb0 99%的紫外线屏蔽,并具有92.5%的DPPH自由基清除能力。应用于纸包装,铁离子配位木质素-金属多酚网络(FL-MPNs, 100 g/m2)具有优异的阻隔和抗氧化性能,同时保持机械强度,将水蒸气透射率降低约40%。在新鲜度测试中,它们将质量损失降低到3.52%,pH变化降低到0.17,有效地抑制了食品的变质。因此,fl - mpn推进木质素增值下一代可持续智能包装,以确保更安全,更稳定的食品保存。
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引用次数: 0
Multi-target antidiabetic peptides from highland barley (Qingke): Identification, molecular docking, and inhibitory mechanisms against α-amylase, α-glucosidase, and DPP-IV 青稞多靶点抗糖尿病肽的鉴定、分子对接及其对α-淀粉酶、α-葡萄糖苷酶和DPP-IV的抑制机制
IF 9.8 1区 农林科学 Q1 CHEMISTRY, APPLIED Pub Date : 2026-04-15 Epub Date: 2026-02-13 DOI: 10.1016/j.foodchem.2026.148412
Juan Li , Muyan Ma , Feng Liang , Yan Du , Wenju Zhou , Pin Ma , Falin Zhang , Zhaoxin Tu , Li Wang , Xinxia Zhang , Ting Li
This study identified three novel multi-target antidiabetic peptides (GFPAGFVFG, YPQPLL, FGFVTF) from Qingke protein hydrolysates. Sequential enzymatic hydrolysis, purification, LC-MS/MS, and molecular docking revealed their strong binding to α-amylase, α-glucosidase, and DPP-IV via hydrophobic and hydrogen-bond interactions. In vitro assays showed potent concurrent inhibition of all three enzymes, with IC₅₀ values of 0.15–1.14, 0.16–0.69, and 0.02–0.75 mg/mL, respectively. Notably, the peptides exhibited a synergistic multi-enzyme inhibitory profile, collectively targeting carbohydrate digestion and incretin degradation pathways—a mechanistic advantage over single-target drugs such as acarbose (α-glucosidase inhibitor) or sitagliptin (DPP-IV inhibitor). Inhibition kinetics further revealed distinct modes of action (competitive, non-competitive, and mixed-type), supporting their potential as multi-target therapeutic candidates. These findings highlight highland barley as a sustainable source of bioactive peptides with synergistic potential for the management of postprandial hyperglycemia and type 2 diabetes.
本研究从清可蛋白水解物中鉴定出三种新的多靶点抗糖尿病肽(GFPAGFVFG, YPQPLL, FGFVTF)。酶解、纯化、LC-MS/MS和分子对接显示,它们通过疏水和氢键相互作用与α-淀粉酶、α-葡萄糖苷酶和DPP-IV强结合。体外实验显示,对所有三种酶都有有效的同时抑制作用,IC₅0值分别为0.15-1.14、0.16-0.69和0.02-0.75 mg/mL。值得注意的是,这些肽表现出协同的多酶抑制特征,共同靶向碳水化合物消化和肠促胰岛素降解途径,这比单靶点药物如阿卡波糖(α-葡萄糖苷酶抑制剂)或西格列汀(DPP-IV抑制剂)具有机制优势。抑制动力学进一步揭示了不同的作用模式(竞争性,非竞争性和混合型),支持它们作为多靶点治疗候选药物的潜力。这些发现强调了青稞作为生物活性肽的可持续来源,对餐后高血糖和2型糖尿病的管理具有协同潜力。
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引用次数: 0
Identification of furan fatty acids in red wines of merlot and cabernet sauvignon: Origin and impact on the formation of 3-methyl-2,4-nonanedione 梅洛和赤霞珠红葡萄酒中呋喃脂肪酸的鉴定:来源及其对3-甲基-2,4-壬二酮形成的影响。
IF 9.8 1区 农林科学 Q1 CHEMISTRY, APPLIED Pub Date : 2026-04-15 Epub Date: 2026-02-18 DOI: 10.1016/j.foodchem.2026.148484
Alexandre Pons , Anita Peterson , Philippe Darriet
3-Methyl-2,4-nonanedione (MND) is an impact oxidation marker of red wines, whose formation mechanisms in wines have received little attention until now. We first studied press wines (PW) containing large amounts of this β-diketone (∼120 ng/L) according to the vintage and the press type, thus suggesting a high level of precursors. The ethyl esters of two fatty acids belonging to the furan fatty acid (FFA) family were identified for the first time: DiMeF(9,5) and DiMeF(11,5). We developed a method for quantifying these compounds by GC-TOF-MS after ethylation. DiMeF(9,5) was detected at trace level whereas the highest DiMeF(11,5) levels reached 50 μg/L in PW. The oxidation of the latter at this concentration explains for the first time the higher MND levels detected in PW. In addition, the first identification of this FFA in Cabernet Sauvignon grapes by GCxGC TOF MS suggests that these new compounds in wines have a varietal origin.
3-甲基-2,4-壬胺二酮(MND)是一种红酒冲击氧化标志物,其在葡萄酒中的形成机制目前还很少有人关注。我们首先根据年份和压榨类型研究了含有大量β-二酮(~ 120 ng/L)的压榨葡萄酒(PW),从而表明高水平的前体。首次鉴定了呋喃脂肪酸(FFA)家族的两种脂肪酸的乙酯:DiMeF(9,5)和DiMeF(11,5)。我们建立了乙基化后的GC-TOF-MS定量方法。DiMeF(9,5)在微量水平检测到,而PW中DiMeF(11,5)的最高水平达到50 μg/L。后者在这个浓度下的氧化首次解释了PW中检测到的较高的MND水平。此外,通过GCxGC TOF MS首次在赤霞珠葡萄中鉴定出该FFA,表明葡萄酒中的这些新化合物具有品种来源。
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引用次数: 0
Global diversity of arabinoxylans, alkylresorcinols and phenolic acids in 180 rye (Secale cereale L.) accessions: implications for functional food innovation 180种黑麦中阿拉伯木聚糖、烷基间苯二酚和酚酸的全球多样性:对功能食品创新的启示
IF 9.8 1区 农林科学 Q1 CHEMISTRY, APPLIED Pub Date : 2026-04-15 Epub Date: 2026-02-17 DOI: 10.1016/j.foodchem.2026.148528
Yuanyuan Tian , Hongyu Zhang , Tiantian Chen , Pengpeng Liu , Jindong Liu , Zhonghu He , Wenfei Tian
Unlike wheat and rice, which are predominantly consumed as refined grains, rye (Secale cereale L.) is mostly consumed as whole grain, making it an ideal vehicle of dietary fiber and phytochemicals. This study profiled arabinoxylans (AX), alkylresorcinols (AR) and phenolic acids in 180 genetically diverse rye accessions from 32 countries. Total AX ranged 70.1–133.0 mg/g, water-extractable AX 6.2–12.7 mg/g, total AR 718.7–2322.2 μg/g and ferulic acid 476.5–1778.1 μg/g on a dry matter basis. Developed cultivars matched or exceeded landraces for total AX (+8%) and ferulic acid (+19%), indicating that modern breeding has not compromised nutritional quality. Correlations were positive among bioactive classes but negative with thousand kernel weight. Elite accessions such as Caribou combined high levels of all three components, offering immediate options for high-fiber, antioxidant-rich product formulation. Overall, these findings broaden the biochemical landscape of rye and identify specific germplasm for functional food innovation.
小麦和大米主要作为精制谷物食用,而黑麦主要作为全谷物食用,使其成为膳食纤维和植物化学物质的理想载体。本研究分析了来自32个国家的180份遗传多样性黑麦材料中的阿拉伯木聚糖(AX)、烷基间苯二酚(AR)和酚酸。干物质基础上,总AX为70.1 ~ 133.0 mg/g,水提AX为6.2 ~ 12.7 mg/g,总AR为718.7 ~ 2322.2 μg,阿魏酸为476.5 ~ 1778.1 μg/g。发达品种的总AX值(+8%)和阿魏酸值(+19%)与地方品种相当或超过地方品种,表明现代育种并未影响其营养品质。各生物活性类间呈正相关,与千粒重呈负相关。精英产品,如Caribou,结合了高水平的这三种成分,提供了高纤维,富含抗氧化剂的产品配方的直接选择。总的来说,这些发现拓宽了黑麦的生化领域,并为功能性食品创新确定了特定的种质资源。
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引用次数: 0
Valorization of beetroot waste through betalains extraction using edible and ready-to-use NADES: A circular economy approach for next-generation health-promoting food colorants 通过食用和即食NADES提取甜菜根废料的价值增值:下一代促进健康的食用色素的循环经济方法
IF 9.8 1区 农林科学 Q1 CHEMISTRY, APPLIED Pub Date : 2026-04-15 Epub Date: 2026-02-05 DOI: 10.1016/j.foodchem.2026.148314
Nevena Kićović , Đurđa Ivković , Maja Krstić Ristivojević , Jasmina Vidic , Jelena Trifković , Filip Andrić , Petar Ristivojević
Concerns about synthetic food dyes being associated with adverse health effects have increased interest in safer, natural alternatives, which provide vivid color and beneficial bioactive properties. This study explores the beetroot waste valorization by extracting bioactive pigments using 15 edible, ready-to-use Natural Deep Eutectic Solvents (NADES). The five best-performing extracts–showing highest total phenolic content, betalain yield and antioxidant capacity–were obtained with betaine/xylitol/1,3-propanediol, betaine/fructose, fructose/glucose/glycerol, fructose/xylitol/glycerol, and choline chloride/citric acid NADES systems, and applied as natural colorants in cookies. Choline chloride/citric acid NADES extract exhibited the strongest anti-diabetic (98 ± 1%), anti-obesity (53 ± 3%), and antimicrobial activity against four foodborne pathogens, enzymatic browning inhibition (98 ± 2%), and produced the most intense coloration in the cookie model system. It showed dose-dependent cytotoxicity, whereas remaining extracts were non-toxic. Тhese findings demonstrate the feasibility of valorizing beetroot by-products into sustainable, health-promoting food colorants aligned with circular-economy and clean-label strategies.
由于担心合成食用色素会对健康造成不良影响,人们对更安全、天然的替代品的兴趣日益浓厚,因为它们能提供鲜艳的颜色和有益的生物活性特性。本研究通过使用15种可食用的天然深共熔溶剂(NADES)提取甜菜根废物的生物活性色素,探索了甜菜根废物的增值。用甜菜碱/木糖醇/1,3-丙二醇、甜菜碱/果糖、果糖/葡萄糖/甘油、果糖/木糖醇/甘油和氯化胆碱/柠檬酸NADES体系得到了5种性能最好的提取物,它们均表现出最高的总酚含量、甜菜碱产率和抗氧化能力。氯化胆碱/柠檬酸NADES提取物具有最强的抗糖尿病(98±1%)、抗肥胖(53±3%)、抗4种食源性致病菌、酶促褐变抑制(98±2%)和饼干模型系统中最强的显色性。其表现出剂量依赖的细胞毒性,而其余提取物则无毒。Тhese研究结果表明,将甜菜根副产品转化为可持续的、促进健康的食用色素,与循环经济和清洁标签战略相一致,是可行的。
{"title":"Valorization of beetroot waste through betalains extraction using edible and ready-to-use NADES: A circular economy approach for next-generation health-promoting food colorants","authors":"Nevena Kićović ,&nbsp;Đurđa Ivković ,&nbsp;Maja Krstić Ristivojević ,&nbsp;Jasmina Vidic ,&nbsp;Jelena Trifković ,&nbsp;Filip Andrić ,&nbsp;Petar Ristivojević","doi":"10.1016/j.foodchem.2026.148314","DOIUrl":"10.1016/j.foodchem.2026.148314","url":null,"abstract":"<div><div>Concerns about synthetic food dyes being associated with adverse health effects have increased interest in safer, natural alternatives, which provide vivid color and beneficial bioactive properties. This study explores the beetroot waste valorization by extracting bioactive pigments using 15 edible, ready-to-use Natural Deep Eutectic Solvents (NADES). The five best-performing extracts–showing highest total phenolic content, betalain yield and antioxidant capacity–were obtained with betaine/xylitol/1,3-propanediol, betaine/fructose, fructose/glucose/glycerol, fructose/xylitol/glycerol, and choline chloride/citric acid NADES systems, and applied as natural colorants in cookies. Choline chloride/citric acid NADES extract exhibited the strongest anti-diabetic (98 ± 1%), anti-obesity (53 ± 3%), and antimicrobial activity against four foodborne pathogens, enzymatic browning inhibition (98 ± 2%), and produced the most intense coloration in the cookie model system. It showed dose-dependent cytotoxicity, whereas remaining extracts were non-toxic. Тhese findings demonstrate the feasibility of valorizing beetroot by-products into sustainable, health-promoting food colorants aligned with circular-economy and clean-label strategies.</div></div>","PeriodicalId":318,"journal":{"name":"Food Chemistry","volume":"508 ","pages":"Article 148314"},"PeriodicalIF":9.8,"publicationDate":"2026-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146135091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Near- and Mid-Infrared Spectroscopy for the Rapid and Non-Destructive Analysis of Wheat Flour and Wheat-Based Products: A Review 近红外和中红外光谱快速无损分析小麦面粉和小麦制品的研究进展
IF 9.8 1区 农林科学 Q1 CHEMISTRY, APPLIED Pub Date : 2026-04-15 Epub Date: 2026-02-11 DOI: 10.1016/j.foodchem.2026.148379
M. Pilar España-Fariñas , Patricia Cazón , María Ángeles Romero-Rodríguez
Wheat flour and its derivatives are staple foods worldwide, making their quality and safety essential for the food industry and consumers. Conventional analytical methods are often slow, costly, and destructive. In recent years, infrared spectroscopy, particularly near-infrared (NIR) and mid-infrared (MIR), has emerged as a rapid, cost-effective, and non-destructive alternative for wheat flour assessment. This review summarizes recent advances in applying these techniques to determine quality parameters, monitor functional properties, detect adulterants, verify authenticity, and evaluate contamination risks. The integration of spectroscopy with chemometric methods and machine learning has improved predictive accuracy and robustness, supporting real-time and in situ analysis. In addition, the use of calibration models, wavelength selection strategies, and deep learning approaches has further enhanced analytical performance. Overall, NIR and MIR spectroscopy stand out as key tools to optimize quality control and safety in wheat flour, contributing to fast, reliable, and sustainable analytical systems for the food industry.
小麦粉及其衍生物是世界各地的主食,其质量和安全对食品工业和消费者至关重要。传统的分析方法往往是缓慢的、昂贵的和破坏性的。近年来,红外光谱,特别是近红外(NIR)和中红外(MIR),已经成为一种快速、经济、无损的小麦面粉评估方法。本文综述了这些技术在确定质量参数、监测功能特性、检测掺假、验证真实性和评估污染风险方面的最新进展。光谱学与化学计量学方法和机器学习的集成提高了预测的准确性和鲁棒性,支持实时和原位分析。此外,校准模型、波长选择策略和深度学习方法的使用进一步提高了分析性能。总的来说,近红外光谱和MIR光谱是优化小麦粉质量控制和安全的关键工具,有助于为食品工业提供快速、可靠和可持续的分析系统。
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引用次数: 0
Synergistically engineered porous wheat starch via sodium trimetaphosphate–sodium hypochlorite dual modification: Microstructural revelations and enhanced functional performance 通过三偏磷酸钠-次氯酸钠双重改性协同工程多孔小麦淀粉:微观结构揭示和增强功能性能
IF 9.8 1区 农林科学 Q1 CHEMISTRY, APPLIED Pub Date : 2026-04-15 Epub Date: 2026-02-11 DOI: 10.1016/j.foodchem.2026.148353
Jun Chen , Huiliang Wen , Weiqi Fei , Zhen Cao , Jia Kong , Jianhua Xie
Porous wheat starch (PWS) holds promise as a functional ingredient, but its application is often limited by its poor physicochemical stability. This study employed sodium hypochlorite oxidation and sodium trimetaphosphate cross-linking to enhance the structural and functional properties of PWS. Results revealed that the A-type crystal structure of modified PWS was preserved at the long-range level, and the formation of carbonyl (CO, 1640 cm−1) and phosphate diester bonds (C-O-P-O-C, 887 cm−1) confirmed the occurrence of oxidation and crosslinking reactions. The dual-modified PWS exhibited substantial improvements in thermal stability, shear resistance, and viscoelastic properties and remarkably enhanced adsorption capacities for water (109.83% ± 3.11%), oil (120.34% ± 3.40%), and proanthocyanidin (6.43 ± 0.19 mg/g)—substantially outperforming single-modified starch. These findings reveal a strong synergistic mechanism for engineering high-performance starch derivatives and establish dual-modified PWS as a promising bioactive compound carrier and versatile food industry functional ingredient.
多孔小麦淀粉(PWS)是一种很有前景的功能性原料,但其物理化学稳定性较差,限制了其应用。本研究采用次氯酸钠氧化和三偏磷酸钠交联来增强PWS的结构和功能特性。结果表明,改性后的PWS在长时间水平上保持了a型晶体结构,羰基键(CO, 1640 cm−1)和磷酸二酯键(C-O-P-O-C, 887 cm−1)的形成证实了氧化和交联反应的发生。双改性后的PWS在热稳定性、抗剪切性和粘弹性方面均有显著改善,对水(109.83%±3.11%)、油(120.34%±3.40%)和原花青素(6.43±0.19 mg/g)的吸附能力显著提高,大大优于单改性淀粉。这些研究结果揭示了工程淀粉衍生物的强大协同机制,并确立了双改性PWS作为一种有前景的生物活性化合物载体和多功能食品工业功能成分。
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引用次数: 0
Dual real-time fluorescent and colorimetric LAMP assay for rapid and on-site identification of sturgeon (Acipenser and Huso) 双实时荧光比色LAMP法快速现场鉴定鲟鱼(Acipenser和Huso)
IF 9.8 1区 农林科学 Q1 CHEMISTRY, APPLIED Pub Date : 2026-04-15 Epub Date: 2026-02-10 DOI: 10.1016/j.foodchem.2026.148391
Chao Ji , Jiaxiu Yao , Yuxiao Lu , Xiangan Li , Sentao Liu , Marti Z. Hua , Henry K. Rotich , Lara Tinacci , Wenjie Zheng , Liangjuan Zhao , Xiaonan Lu
Fraudulent substitution of sturgeon caviar with lower-value roe or non-sturgeon eggs poses risks to consumer protection and sturgeon conservation. In this study, a dual-mode loop-mediated isothermal amplification (LAMP) assay was developed for rapid and on-site authentication of sturgeon (Acipenseridae)-derived components in caviar. This assay integrates SYTO-9 real-time fluorescence and hydroxynaphthol blue colorimetry within a closed-tube system. Primers targeting a conserved region of the Acipenser and Huso cytochrome b gene generated a 190-bp amplicon, enabling detection of sturgeon genomic DNA down to 0.1 pg at 64 °C within 10 min, accompanied by a visible sky-blue color change and fluorescence signal. High analytical specificity was demonstrated with no amplification observed from 14 non-Acipenseridae fish samples. Validation using 17 commercial caviar products showed complete agreement with sequencing-based species identification. The simplicity, speed, and closed-tube format of this assay reduce equipment needs and contamination risks, supporting its suitability for regulatory, commercial, and conservation applications.
用价值较低的鱼子或非鲟鱼卵欺诈性替代鲟鱼鱼子酱对消费者保护和鲟鱼保护构成风险。本研究建立了一种双模环介导等温扩增(LAMP)方法,用于鱼子酱中鲟源成分的快速现场鉴定。该检测将SYTO-9实时荧光和羟基酚蓝比色法集成在一个封闭的管系统中。引物靶向Acipenser和Huso细胞色素b基因的保守区域,产生一个190 bp的扩增子,在64 °C下,在10 min内检测到低至0.1 pg的鲟鱼基因组DNA,并伴有可见的天蓝色变化和荧光信号。结果表明,该方法具有较高的分析特异性,在14份非鲟科鱼类样品中无扩增现象。使用17种商业鱼子酱产品进行验证,结果与基于测序的物种鉴定完全一致。该检测的简单性、速度和闭管格式减少了设备需求和污染风险,支持其适用于监管、商业和保护应用。
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引用次数: 0
A colorimetric-photothermal dual-response immune-sensor constructed with positively charged hollow Bi2S3 submicron star-shaped particles for the detection of dibutyl phthalate 用带正电的空心Bi2S3亚微米星形粒子构建了一种检测邻苯二甲酸二丁酯的比色-光热双响应免疫传感器
IF 9.8 1区 农林科学 Q1 CHEMISTRY, APPLIED Pub Date : 2026-04-15 Epub Date: 2026-02-10 DOI: 10.1016/j.foodchem.2026.148385
Qiong Huang
Dibutyl phthalate (DBP) contamination severely endangers human health and ecosystems. For sensitive and convenient detection of DBP, a colorimetric-photothermal dual-response lateral flow immunoassay (LFIA) based on hollow Bi2S3 submicron star-shaped particles (Bi2S3 HSSPs) was developed. The positively charged Bi2S3 HSSPs synthesized via a one-pot hydrothermal method enable efficient labeling with antibodies. After assembling the test strips, featuring excellent stability, dispersibility, and appropriate particle size, Bi2S3 HSSPs are suitable for chromatographic process and exhibit strong colorimetric signals. Notably, the unique hollow structure and star-like surface endow superior photothermal conversion, providing sensitive temperature responses. The limit of detection (LOD) for DBP is 1.52 ng/mL, with a detection range of 1.56–300 ng/mL and recoveries ranging from 94.2 % to 114.2 %. The Bi2S3 HSSPs as signal labels performed excellently in labeling, chromatography, colorimetry, and photothermal response, and providing a inspirational reference for the selection of labeling materials and improvement of DBP rapid immunoassay.
邻苯二甲酸二丁酯(DBP)污染严重危害人类健康和生态系统。为了灵敏、便捷地检测DBP,建立了一种基于空心Bi2S3亚微米星形颗粒(Bi2S3 HSSPs)的比色-光热双响应横向流动免疫分析法(LFIA)。通过一锅水热法合成的带正电荷的Bi2S3 HSSPs能够有效地标记抗体。组装后的测试条,具有良好的稳定性,分散性和合适的粒度,Bi2S3 hsps适用于色谱过程,并表现出强烈的比色信号。值得注意的是,独特的中空结构和星形表面赋予了优越的光热转换,提供了敏感的温度响应。DBP的检出限为1.52 ng/mL,检测范围为1.56 ~ 300 ng/mL,加样回收率为94.2% ~ 114.2%。作为信号标记物的Bi2S3 HSSPs在标记、色谱、比色和光热响应等方面表现优异,为标记材料的选择和DBP快速免疫分析的改进提供了有益的参考。
{"title":"A colorimetric-photothermal dual-response immune-sensor constructed with positively charged hollow Bi2S3 submicron star-shaped particles for the detection of dibutyl phthalate","authors":"Qiong Huang","doi":"10.1016/j.foodchem.2026.148385","DOIUrl":"10.1016/j.foodchem.2026.148385","url":null,"abstract":"<div><div>Dibutyl phthalate (DBP) contamination severely endangers human health and ecosystems. For sensitive and convenient detection of DBP, a colorimetric-photothermal dual-response lateral flow immunoassay (LFIA) based on hollow Bi<sub>2</sub>S<sub>3</sub> submicron star-shaped particles (Bi<sub>2</sub>S<sub>3</sub> HSSPs) was developed. The positively charged Bi<sub>2</sub>S<sub>3</sub> HSSPs synthesized via a one-pot hydrothermal method enable efficient labeling with antibodies. After assembling the test strips, featuring excellent stability, dispersibility, and appropriate particle size, Bi<sub>2</sub>S<sub>3</sub> HSSPs are suitable for chromatographic process and exhibit strong colorimetric signals. Notably, the unique hollow structure and star-like surface endow superior photothermal conversion, providing sensitive temperature responses. The limit of detection (LOD) for DBP is 1.52 ng/mL, with a detection range of 1.56–300 ng/mL and recoveries ranging from 94.2 % to 114.2 %. The Bi<sub>2</sub>S<sub>3</sub> HSSPs as signal labels performed excellently in labeling, chromatography, colorimetry, and photothermal response, and providing a inspirational reference for the selection of labeling materials and improvement of DBP rapid immunoassay.</div></div>","PeriodicalId":318,"journal":{"name":"Food Chemistry","volume":"508 ","pages":"Article 148385"},"PeriodicalIF":9.8,"publicationDate":"2026-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146153071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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Food Chemistry
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