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Eucommia ulmoides leaf as an alternative substrate for kombucha fermentation: the role of microbial communities in dynamics and metabolic profile. 杜仲叶作为康普茶发酵的替代底物:微生物群落在动力学和代谢剖面中的作用。
IF 9.8 1区 农林科学 Q1 CHEMISTRY, APPLIED Pub Date : 2026-02-05 DOI: 10.1016/j.foodchem.2026.148266
Linfeng Wang, Erfeng Wang, Weipeng Wang, Wei Zhang, Yifan Ge, Xiaokang Cao, Hong Sabrina Tian, Xingke Li, Xianfeng Zhu

Eucommia ulmoides leaf (EUL) was introduced as a novel fermentation substrate to replace traditional tea in kombucha. High-performance liquid chromatography, untargeted metabolomics, and high-throughput sequencing were applied to understand the potential relationships during fermentation. EUL kombucha exhibited slower sugar consumption rate and superior in vitro antioxidant activity compared with traditional kombucha. Total polyphenols, geniposidic acid, pinoresinol diglucoside, and rutin demonstrated an increasing trend, whereas total flavonoids and chlorogenic acid contents considerably decreased. Metabolite profiling revealed 211 positive and 165 negative ion components in samples fermented for 0, 4, and 8 days. Komagataeibacter and Gluconobacter were the dominant bacterial genera while Hanseniaspora, Dekkera, and Kregervanrija dominated the yeast community. The dominant microbial genera could have regulated nucleotide metabolism, pentose and glucuronic acid-related conversions, ascorbic acid and aldehyde metabolism, and flavonoid biosynthesis, leading to changes in the expression of differential metabolites of EUL kombucha. The findings support innovation in kombucha development.

介绍了杜仲叶(EUL)作为一种新的发酵底物来替代传统的康普茶。应用高效液相色谱、非靶向代谢组学和高通量测序来了解发酵过程中的潜在关系。与传统康普茶相比,EUL康普茶具有较低的糖消耗速率和较强的体外抗氧化活性。总多酚、京尼平苷酸、松脂醇二糖苷和芦丁含量呈上升趋势,总黄酮和绿原酸含量明显下降。在发酵0、4和8天的样品中,代谢产物谱显示出211个阳性离子和165个负离子成分。Komagataeibacter和Gluconobacter是优势菌属,而Hanseniaspora、Dekkera和Kregervanrija是优势菌属。优势菌属可能调控核苷酸代谢、戊糖和葡萄糖醛酸相关转化、抗坏血酸和醛代谢以及类黄酮生物合成,导致EUL康普茶差异代谢物表达的变化。这些发现支持了康普茶开发的创新。
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引用次数: 0
Changes in composition and flavor of non-centrifugal sugar after traditional Pan and vacuum evaporation processes. 传统蒸煮和真空蒸煮工艺对非离心糖成分和风味的影响。
IF 9.8 1区 农林科学 Q1 CHEMISTRY, APPLIED Pub Date : 2026-02-05 DOI: 10.1016/j.foodchem.2026.148250
Qiuxiao Li, Zelong Wang, Haoyang Gu, Jian Ying, Shuna Zhao

This study elucidates the thermodynamic-driven flavor transformation mechanisms in non-centrifugal sugar processed via Traditional Pan (TP) and Vacuum Evaporation (VE) systems. Dynamic analysis revealed that 73 volatiles were detected in two processing systems: VE's sealed heating process concentrates pyrazine/aldehyde compounds (such as 2-ethyl-3-methylpyrazine), imparting caramel/nutty flavors; whereas TP's shorter heating duration preserves heat-sensitive furan compounds (such as furfural), yielding fresh/fruity aromas - a conclusion validated by sensory testing. Eight characteristic biomarkers were identified through orthogonal partial least squares discriminant analysis (Orthogonal PLS-DA, OPLS-DA), enabling process differentiation. From the nutritional perspective, more minerals (Ca, Zn) are retained in VE group, while higher vitamin B3 are remained in TP group. However, the content of acrylamide exhibited strong positive correlations with pyrazines/furanones, revealing the Maillard reaction's dual role in flavor enhancement and safety risks. These findings propose a synergistic "flavor-nutrition-safety" control strategy for process optimization, supporting brown sugar industry advancement.

本研究阐明了传统蒸锅(TP)和真空蒸发(VE)两种系统加工非离心糖的热力学驱动风味转化机理。动态分析显示,在两种加工系统中检测到73种挥发物:VE的密封加热过程浓缩了吡嗪/醛化合物(如2-乙基-3-甲基吡嗪),赋予焦糖/坚果味;而TP较短的加热时间保留了热敏感的呋喃化合物(如糠醛),产生新鲜/水果的香味——这一结论得到了感官测试的验证。通过正交偏最小二乘判别分析(orthogonal PLS-DA, OPLS-DA)鉴定出8个特征生物标志物,实现了工艺区分。从营养角度看,VE组保留了较多的矿物质(Ca、Zn), TP组保留了较多的维生素B3。丙烯酰胺含量与吡嗪类/呋喃酮类呈显著正相关,说明美拉德反应具有增强风味和安全风险的双重作用。这些发现为工艺优化提出了一种“风味-营养-安全”协同控制策略,支持红糖行业的发展。
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引用次数: 0
Modifying pea protein via dynamic high-pressure microfluidization and polyphenol/polysaccharide incorporation to stabilize high internal phase Pickering emulsions for 3D printing 通过动态高压微流化和多酚/多糖掺入对豌豆蛋白进行改性,以稳定用于3D打印的高内相Pickering乳剂
IF 8.8 1区 农林科学 Q1 CHEMISTRY, APPLIED Pub Date : 2026-02-05 DOI: 10.1016/j.foodchem.2026.148234
Leichao Dong, Guihua Sheng, Yajie Li, Tingting Guo, Yidan Ni, Haimei Bai, Qinshuo Han, Quancheng Zhou
In this study, pea protein was modified by dynamic high-pressure microfluidization combined with chlorogenic acid and cellulose nanofiber incorporation to synthesize three ternary complexes for stabilizing 3D-printed high-internal-phase Pickering emulsions. DCC12 (pea protein:cellulose nanofiber:chlorogenic acid mass ratio = 1000:20:3) showed the smallest D50 size (4.7 ± 0.2 μm). Cellulose nanofibers maintain the zeta potential of DCC12 at −13.1 ± 0.2 mV; in their absence the zeta potential is reduced to −6.4 ± 0.2 mV. Besides, DCC12 showed good wettability (51.8 ± 1.6°), the highest free sulfhydryl content (1.9 ± 0.1 μmol/g), and the highest antioxidant capacity increase compared with the raw protein (DPPH/ABTS radical scavenging activity: 202.24%/66.16%). The DCC12-based gels exhibited outstanding thermal and centrifugal stabilities owing to interface layer enhancement by polyphenols. Therefore, DCC12 could be used to build gels with dense and stable networks and rheological and textural properties suggesting printability. These findings provide a strategy for the application of pea proteins in novel 3D-printed foods.
本研究采用动态高压微流化技术,结合绿原酸和纤维素纳米纤维对豌豆蛋白进行改性,合成了三种三元配合物,用于稳定3d打印的高内相皮克林乳液。DCC12(豌豆蛋白:纤维素纳米纤维:绿原酸质量比 = 1000:20:3)的D50粒径最小(4.7 ± 0.2 μm)。纤维素纳米纤维将DCC12的zeta电位维持在−13.1 ± 0.2 mV;在没有它们的情况下,zeta电位降至−6.4 ± 0.2 mV。DCC12具有较好的润湿性(51.8 ± 1.6°)、最高的游离巯基含量(1.9 ± 0.1 μmol/g)和最高的抗氧化能力(DPPH/ABTS自由基清除能力:202.24%/66.16%)。由于多酚增强了界面层,dcc12基凝胶表现出优异的热稳定性和离心稳定性。因此,DCC12可用于构建具有致密和稳定网络的凝胶,并且具有流变学和纹理性能,表明可打印性。这些发现为豌豆蛋白在新型3d打印食品中的应用提供了一种策略。
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引用次数: 0
Unraveling the synergy of wheat-based probiotic- fermented modified dietary Fibers, resistant starch, and gluten in constructing high-quality, low Glycemic index noodles 以小麦为基础的益生菌发酵改性膳食纤维,抗性淀粉和面筋在构建高品质,低血糖指数面条中的协同作用
IF 8.8 1区 农林科学 Q1 CHEMISTRY, APPLIED Pub Date : 2026-02-05 DOI: 10.1016/j.foodchem.2026.148294
Ai-Mei Liao, Xiao-ge Liu, Qiao-Qiao Lai, Jia-qi Wang, Kiran Thakur, Long Pan, Yin-Chen Hou, Na Liu, Xian-ming Liu, Zhao-Jun Wei
This study aimed to develop low glycemic index (GI) noodles by incorporating probiotic-fermented modified wheat bran dietary fiber (DF), resistant starch (RS), and gluten (G), further investigate their collaborative effects on construction, molecular network, physicochemical, functional properties, and digestion of noodles in vitro and in vivo. Results indicated that optimized formula (10% DF/20% RS/10%G) significantly reduced GI by 44.2% (p < 0.05), from 88.14 (only wheat flour, WF control) to 49.19 through ternary cooperation mechanism: DF enhanced glucose capture, RS formed crystalline domains and repaired gluten network, while G reinforced the matrix via disulfide crosslinking. Compared to WF control, the novel formulation increased maximum tensile strength by 63.64% and reduced glucose AUC in vivo by 6.1%.Threshold analysis established compositional limits (DF ≤ 10%, G ≤ 10%) to prevent structural disruption. This work provides combined action of DF, RS, and G, suggesting the strategy for functional low - GI staple development.
本研究旨在通过添加益生菌发酵改性麦麸膳食纤维(DF)、抗性淀粉(RS)和谷蛋白(G)来开发低血糖指数(GI)面条,并进一步研究它们在面条的体外和体内结构、分子网络、理化、功能特性和消化方面的协同作用。结果表明,优化后的配方(10% DF/20% RS/10%G)通过三元协同机制显著降低GI值44.2% (p <; 0.05),GI值从88.14(仅小麦粉,WF对照)降至49.19,DF增强葡萄糖捕获,RS形成晶体结构域并修复面筋网络,G通过二硫交联增强基质。与WF对照相比,新配方的最大抗拉强度提高了63.64%,体内葡萄糖AUC降低了6.1%。阈值分析建立了成分限值(DF ≤ 10%,G ≤ 10%)以防止结构破坏。这项工作提供了DF, RS和G的联合作用,提出了功能性低GI短纤维的发展策略。
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引用次数: 0
Transforming food authenticity testing by the exploitation of a machine learning – Data fusion approach: a tea case study 利用机器学习-数据融合方法转变食品真实性测试:茶叶案例研究
IF 8.8 1区 农林科学 Q1 CHEMISTRY, APPLIED Pub Date : 2026-02-05 DOI: 10.1016/j.foodchem.2026.148302
Yicong Li, Natasha Logan, Awanwee Petchkongkaew, Yunhe Hong, Xiaotong Liu, Nicholas Birse, Simon Haughey, Terry F. McGrath, Di Wu, Christopher T. Elliott
The growing vulnerability of the global food supply chain highlights the need for rapid, accurate, and non-destructive authenticity testing. In this study, we developed and validated a workflow integrating Fourier-transform infrared, near-infrared, and X-ray fluorescence spectroscopy with machine learning–based data fusion for black tea authentication. A total of 532 authentic Assam, Darjeeling, Ceylon, and Keemun samples were analysed using five supervised models. A series of information-level, feature-level, and decision-level fusion strategies were developed and compared, with decision-level fusion achieving 100% F1 scores across calibration, validation, and test sets, outperforming individual and other fused methods. The workflow was further applied to 89 commercial teas, identifying a 6.74% non-compliance rate, all from online platforms. This approach eliminates the need for expensive mass spectrometry or stable isotope–based instrumentation and is well suited for accurate, cost-effective food authenticity testing in non-specialist laboratories, particularly in developing countries.
全球食品供应链日益增长的脆弱性凸显了对快速、准确和非破坏性真实性检测的需求。在这项研究中,我们开发并验证了一种将傅里叶变换红外、近红外和x射线荧光光谱与基于机器学习的数据融合相结合的工作流程,用于红茶认证。使用五种监督模型分析了532个阿萨姆邦、大吉岭、锡兰和基蒙的正宗样本。开发并比较了一系列信息级、特征级和决策级融合策略,决策级融合在校准、验证和测试集上获得100%的F1分数,优于个体和其他融合方法。该工作流程进一步应用于89种商业茶叶,确定了6.74%的不合规率,全部来自在线平台。这种方法消除了昂贵的质谱法或稳定同位素仪器的需要,非常适合在非专业实验室,特别是在发展中国家进行准确、具有成本效益的食品真实性检测。
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引用次数: 0
Seven neonicotinoids and twenty-two metabolites in seven food matrices by optimized QuEChERS-HPLC-MS/MS and dietary risk assessment 7种食物基质中7种新烟碱类和22种代谢物的优化QuEChERS-HPLC-MS/MS及膳食风险评估
IF 8.8 1区 农林科学 Q1 CHEMISTRY, APPLIED Pub Date : 2026-02-04 DOI: 10.1016/j.foodchem.2026.148256
Zhenzhen Zhou, Xueyan Zhao, Junfeng Lu, Chengkui Qiao, Caixia Wang, Tao Pang, Linlin Guo, Jun Li, Rongli Pang, Hanzhong Xie, Fajun Tian
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引用次数: 0
Influence of plant age and sunlight exposure on the volatile profile and metabolomic pathways of Ilex guayusa leaves used in the traditional Amazonian beverage 植物年龄和阳光照射对传统亚马逊饮料中使用的愈青叶挥发性特征和代谢组学途径的影响
IF 8.8 1区 农林科学 Q1 CHEMISTRY, APPLIED Pub Date : 2026-02-04 DOI: 10.1016/j.foodchem.2026.148238
Jefferson V. Pastuña-Fasso, Melanie Ochoa-Ocampo, Thomas Garzón, Edison Gonzales, Nina Espinosa de los Monteros-Silva, Zulay Niño-Ruíz, Karel Dieguez-Santana, Noroska G.S. Mogollón
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引用次数: 0
Evaluating amino acid fortification in low-dairy ready-to-use therapeutic food formulations: Insights from a meta-analysis re-evaluation 评估低乳制品即食治疗性食品配方中的氨基酸强化:来自荟萃分析重新评估的见解
IF 8.8 1区 农林科学 Q1 CHEMISTRY, APPLIED Pub Date : 2026-02-04 DOI: 10.1016/j.foodchem.2026.148316
Melkamu Berhane, Alemayehu Teklu Toni, Gerard Bryan Gonzales
Severe wasting is treated with ready-to-use therapeutic food (RUTF), wherein the protein content primarily comes from milk and peanuts. As these ingredients are the main drivers of RUTF's cost, global initiatives seek alternatives using locally available, sustainable ingredients to reduce cost and increase coverage. To compensate for reduced/removed dairy protein, current practice is to add amino acids to meet Codex protein quality requirements. In this meta-analysis re-evaluation, we compared RUTF formulations with <50% dairy protein, with or without amino acid fortification, against standard milk–peanut RUTF for the outcome of weight gain. Growth outcomes were similar between fortified and unfortified RUTFs when compared to standard RUTF. These findings suggest that elevating protein quality scores by adding amino acids may be insufficient to ensure clinical efficacy in novel RUTF formulations. Additionally, the findings suggest that Protein Digestibility Corrected Amino Acid Score (PDCAAS) compliance alone may not guarantee clinical efficacy. Future formulation efforts should address digestibility, antinutritional factors, and increasing total protein content to achieve effective, sustainable novel RUTF formulations.
严重的消瘦是用即食治疗食品(RUTF)治疗的,其中的蛋白质含量主要来自牛奶和花生。由于这些成分是RUTF成本的主要驱动因素,全球倡议寻求使用当地可用的可持续成分的替代品,以降低成本并增加覆盖范围。为了弥补减少/去除的乳蛋白,目前的做法是添加氨基酸以满足食典委蛋白质质量要求。在这项荟萃分析重新评估中,我们比较了含有50%乳蛋白的RUTF配方,有或没有氨基酸强化,与标准牛奶花生RUTF的体重增加结果。与标准RUTF相比,强化和未强化RUTF之间的生长结果相似。这些发现表明,通过添加氨基酸来提高蛋白质质量评分可能不足以确保新型RUTF配方的临床疗效。此外,研究结果表明,单独的蛋白质消化率校正氨基酸评分(PDCAAS)依从性可能不能保证临床疗效。未来的配方工作应解决消化率、抗营养因子和增加总蛋白质含量,以实现有效、可持续的新型RUTF配方。
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引用次数: 0
Linkage-resolved profiling of isomalto-oligosaccharides using porous graphitic carbon liquid chromatography–orbitrap tandem mass spectrometry 用多孔石墨碳液相色谱-轨道阱串联质谱分析异麦芽糖低聚糖
IF 8.8 1区 农林科学 Q1 CHEMISTRY, APPLIED Pub Date : 2026-02-04 DOI: 10.1016/j.foodchem.2026.148278
Chih-Chieh Lin, Meng-Jui Lin, Ju-Hua Chou, Pei-Yin Lin, Ting-Jang Lu
The digestibility and glycemic response of isomalto-oligosaccharides are determined by their glycosidic linkage and chain length. However, linkage-resolved profiling beyond tetrasaccharides remains limited. Herein, forty structures from disaccharides to hexasaccharides in eight commercial materials were characterized using porous graphitic carbon liquid chromatography–Orbitrap tandem mass spectrometry. Linkage profiles were dominated by α-1 → 6 and α-1 → 4, displaying a chain-length-dependent shift. Generally, α-1 → 4 linkages accounted for ~10% of total linkages in disaccharides, rising to ~80% in hexasaccharides. In one sample, α-1 → 4 linkages were 1.6-fold more abundant than α-1 → 6. With contents of 62.5–81.5%, no product met the European Food Safety Authority description for isomaltose plus trisaccharide–nonasaccharide content (≥90% dry basis). Even in products labeled “total isomalto-oligosaccharides ≥90% (dry basis),” α-1 → 6 linkages accounted for only ~50% of glycosidic linkages. This suggests the need for linkage-resolved isomalto-oligosaccharide labeling frameworks that extend beyond total content claims to more precisely reflect the digestibility and glycemic impact.
异麦芽糖低聚糖的消化率和升糖反应是由其糖苷键和链长决定的。然而,超越四糖的键解析分析仍然有限。本文采用多孔石墨碳液相色谱-轨道阱串联质谱法对8种商品材料中从双糖到六糖的40种结构进行了表征。连锁谱以α-1 → 6和α-1 → 4为主,呈现链长依赖的移位。一般α-1 → 4键在双糖中占总键数的~10%,在六糖中占~80%。在一个样本中,α-1 → 4的连锁度是α-1 → 6的1.6倍。含量为62.5-81.5%,没有产品符合欧洲食品安全局对异麦芽糖加三糖-非糖含量(≥90%干基)的描述。即使在标有“总异麦芽糖-寡糖≥90%(干基)”的产品中,α-1 → 6键也仅占糖苷键的约50%。这表明有必要建立连接分解的异麦芽糖-低聚糖标签框架,以更准确地反映消化率和血糖影响,而不仅仅是总含量声明。
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引用次数: 0
Fermentation of blueberry slurry by lactobacilli strains: Investigation on soluble and insoluble fractions 乳酸菌发酵蓝莓浆液:可溶性和不溶性组分的研究
IF 8.8 1区 农林科学 Q1 CHEMISTRY, APPLIED Pub Date : 2026-02-04 DOI: 10.1016/j.foodchem.2026.148309
Yi Zhong, Jian Zhang, Qiyue Zhao, Sivakumar Manickam, Yue Wu, Yongbin Han, Shichang Shao, Yang Tao
This study investigated the fermentation of blueberry slurry by Lactiplantibacillus plantarum and Limosilactobacillus fermentum, focusing on three fractions: soluble components in the juice, soluble components adhered to the pomace, and insoluble components within the pomace. Soluble components including sugars, organic acids and phenolics were consistently retained on the blueberry pomace throughout fermentation. Meanwhile, lactic acid fermentation induced irregular changes in the pectin composition of the extracted blueberry cell wall materials (B-CWM) and weakened B-CWM's ability to adsorb free phenolics. After 30-min adsorption in fresh blueberry juice, the adsorption capacity of B-CWM extracted from blueberry pomace fermented by both strains for 48 h were declined by over 50%, compared to unfermented B-CWM. Moreover, significant correlations between microbial activities and pomace physicochemical properties suggest the certain influence of blueberry pomace on the lactic acid fermentation process. These findings provide new insight into how fruit pomace modulates microbial fermentation and phenolic retention.
本研究利用植物乳杆菌和发酵乳酸杆菌对蓝莓浆料进行了发酵研究,重点研究了果汁中的可溶性成分、果渣中粘附的可溶性成分和果渣中不溶性成分三个部分。可溶性成分包括糖、有机酸和酚类物质在整个发酵过程中始终保留在蓝莓果渣上。同时,乳酸发酵导致提取的蓝莓细胞壁材料(B-CWM)的果胶组成发生不规则变化,削弱了B-CWM对游离酚类物质的吸附能力。在新鲜蓝莓汁中吸附30 min后,两菌株发酵48 h提取的蓝莓果渣B-CWM的吸附量比未发酵的B-CWM下降了50%以上。此外,微生物活性与果渣理化性质之间存在显著的相关性,表明蓝莓果渣对乳酸发酵过程有一定的影响。这些发现为果渣如何调节微生物发酵和酚保留提供了新的见解。
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引用次数: 0
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Food Chemistry
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