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Comparative assessment of drying techniques on the quality of millet (Sorghum bicolor) microgreens 谷子(高粱双色)微蔬菜干燥工艺品质的比较评价
IF 8.8 1区 农林科学 Q1 CHEMISTRY, APPLIED Pub Date : 2026-02-09 DOI: 10.1016/j.foodchem.2026.148375
B. Praveena, Mamtha Shafika, Anuja Divate, J.A. Moses
Microgreens are increasingly popular due to their unique nutritional profile, diverse phytochemical composition, and intense flavor. Considering their rapid senescence, this study examined oven drying (OD), conductive hydro drying (CHD), and freeze-drying (FD) to retain the quality of sorghum microgreens. All dried samples exhibited quality changes, with fresh (F) samples serving as the control. FD sample resulted in low moisture (4.34 ± 0.08) and water activity (0.42 ± 0.01), as well as the highest phenolic (40.92 ± 0.18 mg/g) and flavonoid (10.10 ± 0.12 mg/g) contents. Both FD and CHD yielded excellent flow properties; CHD provided higher ascorbic acid retention (12.42 ± 0.38 mg/g). F had 58.42 mg/100 g of total chlorophyll and 5.97 mg/100 g of carotenoids, and the FD sample had the highest pigment content (54.62% more than OD, 42.22% more than CHD). The Midilli-Kucuk model demonstrated the best fit (R2 > 0.99). In summary, while FD offers a higher quality, CHD is a time- and cost-effective technique.
由于其独特的营养成分、多样的植物化学成分和浓郁的风味,微型蔬菜越来越受欢迎。考虑到高粱微绿叶菜的快速衰老,本研究考察了烘箱干燥(OD)、导电水力干燥(CHD)和冷冻干燥(FD)对其品质的影响。所有干燥的样品都表现出质量变化,新鲜(F)样品作为对照。FD样本导致低水分(4.34 ±0.08 )和水活动(0.42 ±0.01 ),以及酚醛最高(40.92 ±0.18  毫克/克)和类黄酮(10.10 ±0.12  毫克/克)的内容。FD和CHD均具有优异的流动性能;冠心病患者抗坏血酸潴留较高(12.42 ± 0.38 mg/g)。F样品的总叶绿素含量为58.42 mg/100 g,类胡萝卜素含量为5.97 mg/100 g, FD样品的色素含量最高(比OD高54.62%,比CHD高42.22%)。midli - kuucuk模型拟合最佳(R2 >; 0.99)。综上所述,虽然FD提供更高的质量,但CHD是一种时间和成本效益高的技术。
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引用次数: 0
Respiratory-responsive active elimination of microbe and pesticide residue on fruits 呼吸反应性主动消除水果上的微生物和农药残留
IF 8.8 1区 农林科学 Q1 CHEMISTRY, APPLIED Pub Date : 2026-02-09 DOI: 10.1016/j.foodchem.2026.148374
Liying Yang, Jia Kong, Douxin Xiao, Ming Du, Alideertu Dong, Peng Yang
Microbial spoilage and excessive pesticide residues pose significant health risks in fresh fruits. This study introduces a concept of active elimination of pesticide residues and microbes on fruit by cellular respiration-stimulated ROS release. For this aim, the biocompatible Zn@metal-organic frameworks (Zn@MOFs) are constructed to autonomously produce reactive oxygen species without light activation, driven by ambient oxygen and moisture activated through fruit cellular respiration. The in situ produced ROS concurrently enhances broad-spectrum antimicrobial activity towards fungi and bacteria. With the help of pectin, the Zn@MOFs could form a uniform and robust protective layer on fruit, adsorb pesticides on the fruit surface, and significantly extend the freshness of non-climacteric fruits and climacteric fruits. Metabolomics analysis reveals that such active coating suppresses key ripening-associated metabolic pathways, including amino acid metabolism and ABC transporter activity. The coating offers a promising, innovative approach for developing next-generation active food packaging.
新鲜水果的微生物腐败和过量农药残留对健康构成重大威胁。本研究介绍了通过细胞呼吸刺激活性氧释放来主动清除水果上的农药残留和微生物的概念。为此,构建了生物相容性Zn@metal-organic框架(Zn@MOFs),在没有光激活的情况下自主产生活性氧,由周围的氧气和水分驱动,通过水果细胞呼吸激活。原位产生的活性氧同时增强了对真菌和细菌的广谱抗菌活性。Zn@MOFs在果胶的帮助下,可以在果实表面形成均匀而坚固的保护层,吸附果实表面的农药,显著延长非更年期水果和更年期水果的新鲜度。代谢组学分析表明,这种活性包衣抑制了关键的成熟相关代谢途径,包括氨基酸代谢和ABC转运蛋白活性。该涂层为开发下一代活性食品包装提供了一种有前途的创新方法。
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引用次数: 0
Softening mechanism of vinasse grass carp during fermentation: The synergistic interaction among water distribution, skeletal proteins, and muscle tissues 酒渣草鱼发酵软化机理:水分分布、骨骼蛋白和肌肉组织的协同作用
IF 8.8 1区 农林科学 Q1 CHEMISTRY, APPLIED Pub Date : 2026-02-09 DOI: 10.1016/j.foodchem.2026.148370
Jinggui Nie, Yong Wang, Xiao Zhang, Dan Wu, Qilin Huang
This study aimed to elucidate the softening mechanism during the fermentation of vinasse grass carp (VGC) based on the synergistic interaction among water, skeletal proteins, and muscle tissues. The transverse and longitudinal shear forces of fish muscle initially decreased and subsequently increased during fermentation, with overall reductions of 64.57% and 58.24%, respectively. In the early stage, the proportions of immobilized and free water increased. Moreover, myofibrillar protein (MP) and collagen gradually degraded with the reduction of their ordered secondary structures (α-helices and β-sheets), leading to the expansion of intermyofibrillar gaps and subsequent muscle softening. In the later stage, MP underwent further degradation, and its surface hydrophobicity increased sharply. Additionally, the epimysium and perimysium were severely damaged and lost their wrapping function, which facilitated the aggregation of muscle fibers into a compact structure, consequently increasing the shear force. This study provides novel insights into the texture-regulating mechanisms of fermented fish products.
本研究旨在从水、骨骼蛋白和肌肉组织三者协同作用的角度探讨酒渣草鱼(VGC)发酵过程中的软化机理。在发酵过程中,鱼肌肉的横向和纵向剪切力先降低后升高,总体降低幅度分别为64.57%和58.24%。在早期,固定水和自由水的比例增加。此外,肌原纤维蛋白(MP)和胶原蛋白随着有序二级结构(α-螺旋和β-片)的减少而逐渐降解,导致肌原纤维间隙扩大,肌肉软化。在后期,MP进一步降解,其表面疏水性急剧增加。此外,肌外膜和肌周膜严重受损,失去包裹功能,使肌纤维聚集成致密结构,从而增加了剪切力。该研究为发酵鱼制品的结构调节机制提供了新的见解。
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引用次数: 0
Corrigendum to “Lipid-protein synergistic micronized salt composite: High-pressure homogenization for enhanced sodium dispersion and thermal stability” [Food Chem. 487 (2025) 144825] “脂质-蛋白质协同微粉盐复合材料:用于增强钠分散和热稳定性的高压均质”的勘误表[食品化学]. 487 (2025)144825]
IF 8.8 1区 农林科学 Q1 CHEMISTRY, APPLIED Pub Date : 2026-02-09 DOI: 10.1016/j.foodchem.2026.148267
Xin Fan, Chengcheng Xu, Yao Qin, Dayong Zhou, Deyang Li
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引用次数: 0
Effects of moisture content on the physicochemical properties and swallowing function of surimi gel 水分含量对鱼糜凝胶理化性质及吞咽功能的影响
IF 8.8 1区 农林科学 Q1 CHEMISTRY, APPLIED Pub Date : 2026-02-09 DOI: 10.1016/j.foodchem.2026.148372
Lixiang Zhu, Lijun Zha, Haozhe Yi, Bowen Li, Xiaowen Pi, Dongling Qiao, Binjia Zhang
Dysphagia presents a critical challenge for dietary management. This study investigated the effects of moisture content (50%–110%) on the physicochemical properties and swallowing safety of surimi gels. Methodologies included the International Dysphagia Diet Standardization Initiative (IDDSI) assessment, in vitro Cambridge pharyngeal simulation, rheology, and microstructural analysis. Results demonstrated that all samples met IDDSI Level 6 criteria. Elevated moisture content significantly shortened oral transit time and reduced oropharyngeal residue. This improvement was attributed to decreased hardness, adhesiveness, zero-shear viscosity, and yield stress, resulting from a looser porous network. Principal component analysis (PCA) identified hardness and adhesiveness as critical determinants of swallowing. This work provides a scientific basis for optimizing surimi gels for dysphagia management via moisture regulation.
吞咽困难是饮食管理的一个关键挑战。本研究考察了水分含量(50% ~ 110%)对鱼糜凝胶理化性质和吞咽安全性的影响。方法包括国际吞咽困难饮食标准化倡议(IDDSI)评估,体外剑桥咽模拟,流变学和微观结构分析。结果表明,所有样品均符合IDDSI 6级标准。提高水分含量可显著缩短口腔运输时间,减少口咽残留。这种改善是由于疏松的多孔网络降低了硬度、粘附性、零剪切粘度和屈服应力。主成分分析(PCA)确定了硬度和粘附性是吞咽的关键决定因素。本研究为优化鱼糜凝胶通过调节水分来治疗吞咽困难提供了科学依据。
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引用次数: 0
Protein fingerprinting using gold nanoparticle-assisted SPE and HPLC-UV: A promising tool for chemometric authentication of monofloral honeys 金纳米颗粒辅助固相萃取和高效液相色谱-紫外光谱的蛋白质指纹图谱:一种有前景的单花蜂蜜化学计量鉴定工具
IF 8.8 1区 农林科学 Q1 CHEMISTRY, APPLIED Pub Date : 2026-02-08 DOI: 10.1016/j.foodchem.2026.148333
Paola Teresa Ogando-Rivas, Isabel Escriche, Ernesto Francisco Simó-Alfonso, Enrique Javier Carrasco-Correa
Authentication of honey according to its botanical origin is essential for quality control. Traditional melissopalynological methods are time-consuming and subjective, motivating the search for faster alternatives. In this work, HPLC-UV protein fingerprinting approach was developed to classify honeys of different floral origins. Previously, proteins were isolated and preconcentrated using a gold nanoparticle-assisted solid-phase extraction. 61 Spanish honey samples from chestnut, heather, and thyme origins were studied where 27 different bee-derived proteins were identified. Distinct protein fingerprint patterns were observed where heather showed the most diverse SPE protein fingerprint, chestnut honey a more balanced distribution and thyme honey displayed higher levels of stress-related proteins. A linear discriminant analysis model was developed using sum-normalized protein abundances. The model was trained with 40 samples and externally validated with 21 independent samples, achieving 100% correct classification. The results demonstrate that bee-origin proteins reflect floral influences and can serve as stable biomarkers for honey authentication.
根据蜂蜜的植物来源进行鉴定对质量控制至关重要。传统的同源学方法耗时且主观,促使人们寻找更快的替代方法。本研究采用HPLC-UV蛋白指纹图谱方法对不同花源的蜂蜜进行分类。以前,蛋白质是用金纳米颗粒辅助固相萃取分离和预浓缩的。研究人员对61份来自栗子、石南花和百里香的西班牙蜂蜜样本进行了研究,发现了27种不同的蜜蜂衍生蛋白质。在不同的蛋白质指纹图谱中,石楠花显示出最多样化的SPE蛋白质指纹图谱,栗子蜜分布更平衡,百里香蜜显示出更高水平的压力相关蛋白质。利用蛋白质丰度和归一化建立了线性判别分析模型。模型训练了40个样本,外部验证了21个独立样本,分类准确率达到100%。结果表明,蜂源蛋白反映了花的影响,可以作为蜂蜜鉴定的稳定生物标志物。
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引用次数: 0
Quantitative Western blot assay for the detection of chicken serum albumin (Gal d 5) 鸡血清白蛋白(gald5)的定量Western blot检测
IF 8.8 1区 农林科学 Q1 CHEMISTRY, APPLIED Pub Date : 2026-02-08 DOI: 10.1016/j.foodchem.2026.148325
Yaqi Zhao, Aravind Kumar Bingi, Qinchun Rao
Egg is one of the Big-Nine allergenic foods in the U.S., and accurate detection of its allergenic proteins is critical for food safety. Chicken serum albumin (CSA, Gal d 5), an allergen found in egg yolk, chicken meat, and avian-derived materials, remains under-detected in food products. This study aimed to characterize CSA and develop a quantitative Western blot (qWB) assay for the detection of CSA. After characterization, (1) high temperature (>69.61 °C) decreased CSA extractability, (2) in vitro allergenicity was detected by human plasma, and (3) CSA was verified in egg white. A monoclonal antibody 3H4E9 was characterized as specific to poultry serum albumin. The optimized qWB assay exhibited good selectivity (specific to poultry species), working range (0.3–20 ppm), and sensitivity (ppm level). This assay enables sensitive and reliable CSA detection on food contact surfaces, facilitating risk assessment of undeclared egg yolk residues and improving food safety management practices.
鸡蛋是美国九大致敏食品之一,准确检测其致敏蛋白对食品安全至关重要。鸡血清白蛋白(CSA, Gal d 5)是一种在蛋黄、鸡肉和禽类衍生材料中发现的过敏原,在食品中仍未被检测到。本研究旨在对CSA进行表征,并建立CSA的定量Western blot (qWB)检测方法。经鉴定,(1)高温(>69.61 °C)降低了CSA的可提取性,(2)人血浆检测体外致敏性,(3)在蛋清中验证了CSA。一种单克隆抗体3H4E9对家禽血清白蛋白具有特异性。优化后的qWB法具有良好的选择性(对禽类品种有特异性)、工作范围(0.3-20 ppm)和灵敏度(ppm水平)。该方法能够对食品接触面进行敏感可靠的CSA检测,促进未申报蛋黄残留物的风险评估,提高食品安全管理水平。
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引用次数: 0
Identification of roasting degree and interpretability analysis of Yunnan arabica coffee beans based on multi-dimensional visual features and CNNs-SHAP 基于多维视觉特征和cnn - shap的云南阿拉比卡咖啡豆烘焙度鉴定及可解释性分析
IF 9.8 1区 农林科学 Q1 CHEMISTRY, APPLIED Pub Date : 2026-02-08 DOI: 10.1016/j.foodchem.2026.148367
Siheng Lu , Jing Zhao , Qian Qin , Wenxuan Deng , Yue Yu , Yue Huang , Ming Li , Hao Dong , Zhanming Li
This study extracted Yunnan Arabica coffee bean features through weighted fusion of CIE L*a*b* color histograms, Gray-level Co-occurrence Matrix-Local Binary Pattern composite textures, and morphological parameters, aiming to achieve accurate roasting degree identification and transparent decision-making. Convolutional neural networks outperformed all other models with the highest accuracy. Subsequent SHapley Additive exPlanations analysis demonstrated that key features exhibited a significant strong negative linear correlation with prediction outputs; negative contributions dominated light roasting samples, whereas robust positive contributions dominated dark roasting samples. External verification results clarified that the accuracy of dark-roasted samples reached 100.0%, that of light-roasted samples was 91.5%, and that of medium-roasted samples was 93.8%. The proposed intelligent system enabled automatic image acquisition, feature extraction, and inference, and supported connection to the Enterprise Resource Planning system for data traceability. This study broke deep learning's black-box limitation, providing a precise interpretable technique for coffee roasting standardization.
本研究通过CIE L*a*b*颜色直方图、灰度共生矩阵-局部二值模式复合纹理、形态参数加权融合提取云南阿拉比卡咖啡豆特征,实现烘焙度的准确识别和透明决策。卷积神经网络以最高的准确率优于所有其他模型。随后的SHapley加性解释分析表明,关键特征与预测结果呈显著的强负线性相关;负贡献以轻度焙烧样品为主,而强正贡献以深色焙烧样品为主。外部验证结果表明,深焙样品的准确度为100.0%,浅焙样品的准确度为91.5%,中焙样品的准确度为93.8%。提出的智能系统支持自动图像采集、特征提取和推理,并支持与企业资源规划系统的连接,以实现数据可追溯性。这项研究打破了深度学习的黑盒限制,为咖啡烘焙标准化提供了一种精确的可解释技术。
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引用次数: 0
Determination of vitamin K1 (phylloquinone) and K2 (menaquinones 4–10) in a broad range of matrices by HPLC with electrochemical reduction and fluorescence detection 电化学还原-荧光高效液相色谱法测定多种基质中维生素K1(叶绿醌)和K2(萘醌4-10)的含量
IF 8.8 1区 农林科学 Q1 CHEMISTRY, APPLIED Pub Date : 2026-02-08 DOI: 10.1016/j.foodchem.2026.148327
Kari Elin Rød karielin, Annbjørg Bøkevoll, Amalie Moxness Reksten, Lisbeth Dahl, Eystein Oveland
An analytical method for simultaneous determination of cis−/trans-forms of vitamin K1 (phylloquinone), β, γ-dihydro K1 and K2 (menaquinones 4–10) was developed and thoroughly validated for applicability to a broad range of food matrices, tissue fluids, and supplements. The extraction procedures and post-liquid-liquid extraction solvents were optimized to maximize recovery that ranged from 80 to 118%. A longer HPLC column and a gradient were applied for improved separation of vitamers as well as cis/trans-forms. External standard curves for the vitamin K forms were efficiently injected by an autosampler (R2 ≥ 0.999). Vitamin K was efficiently reduced to hydroquinone by an online post-column electrochemical reduction cell before fluorescence detection. By analyzing reference materials and spiked samples the average LOQs were approximately 0.1 μg/kg for all vitamers except for 0.2 μg/kg for MK-6 and MK-10. The intra- and inter-day precision, expressed as 2RSD, were typically around 8% for K1 and 10% for the K2 forms.
建立了一种同时测定维生素K1(叶绿醌)、β、γ-二氢K1和K2(甲基萘醌4-10)顺式/反式的分析方法,并对其适用于广泛的食品基质、组织液和补充剂进行了全面验证。优化提取工艺和液-液后萃取溶剂,回收率为80% ~ 118%。采用更长的高效液相色谱柱和梯度,以提高维生素和顺式/反式的分离。自进样器有效地注入了维生素K形式的外部标准曲线(R2 ≥ 0.999)。在荧光检测前,利用在线柱后电化学还原池将维生素K有效还原为对苯二酚。除MK-6和MK-10的loq为0.2 μg/kg外,其余维生素的loq均为0.1 μg/kg。以2RSD表示的日内和日间精度,K1形式通常约为8%,K2形式约为10%。
{"title":"Determination of vitamin K1 (phylloquinone) and K2 (menaquinones 4–10) in a broad range of matrices by HPLC with electrochemical reduction and fluorescence detection","authors":"Kari Elin Rød karielin, Annbjørg Bøkevoll, Amalie Moxness Reksten, Lisbeth Dahl, Eystein Oveland","doi":"10.1016/j.foodchem.2026.148327","DOIUrl":"https://doi.org/10.1016/j.foodchem.2026.148327","url":null,"abstract":"An analytical method for simultaneous determination of <em>cis</em>−/<em>trans</em>-forms of vitamin K1 (phylloquinone), β, γ-dihydro K1 and K2 (menaquinones 4–10) was developed and thoroughly validated for applicability to a broad range of food matrices, tissue fluids, and supplements. The extraction procedures and post-liquid-liquid extraction solvents were optimized to maximize recovery that ranged from 80 to 118%. A longer HPLC column and a gradient were applied for improved separation of vitamers as well as <em>cis</em>/<em>trans</em>-forms. External standard curves for the vitamin K forms were efficiently injected by an autosampler (R<sup>2</sup> ≥ 0.999). Vitamin K was efficiently reduced to hydroquinone by an online post-column electrochemical reduction cell before fluorescence detection. By analyzing reference materials and spiked samples the average LOQs were approximately 0.1 μg/kg for all vitamers except for 0.2 μg/kg for MK-6 and MK-10. The intra- and inter-day precision, expressed as 2RSD, were typically around 8% for K1 and 10% for the K2 forms.","PeriodicalId":318,"journal":{"name":"Food Chemistry","volume":"43 1","pages":""},"PeriodicalIF":8.8,"publicationDate":"2026-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146138845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
MOF-based nanozyme incorporated composite film with prominent antibacterial activity and multiple functionalities for fruit preservation 基于mof的纳米酶具有优异的抗菌活性和多种水果保鲜功能
IF 8.8 1区 农林科学 Q1 CHEMISTRY, APPLIED Pub Date : 2026-02-08 DOI: 10.1016/j.foodchem.2026.148360
Yiyi Shi, Mengmeng Pan, Yan Wei, Wanchun Luo, Ying Ma, Liyun Ma, Ming Jiang, Feng Liu, Xu Yu, Li Xu
{"title":"MOF-based nanozyme incorporated composite film with prominent antibacterial activity and multiple functionalities for fruit preservation","authors":"Yiyi Shi, Mengmeng Pan, Yan Wei, Wanchun Luo, Ying Ma, Liyun Ma, Ming Jiang, Feng Liu, Xu Yu, Li Xu","doi":"10.1016/j.foodchem.2026.148360","DOIUrl":"https://doi.org/10.1016/j.foodchem.2026.148360","url":null,"abstract":"","PeriodicalId":318,"journal":{"name":"Food Chemistry","volume":"182 1","pages":""},"PeriodicalIF":8.8,"publicationDate":"2026-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146138768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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Food Chemistry
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