Food Control最新文献_第8页

Chalcone derivative enhance poultry meat preservation through quorum sensing inhibition against Salmonella (Salmonella enterica serovar Typhi) contamination

IF 5.6 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Control

Pub Date : 2025-01-13 DOI: 10.1016/j.foodcont.2025.111155

S.P. Ramya Ranjan Nayak , Pratik Pohokar , Anamika Das , L.S. Dhivya , Mukesh Pasupuleti , Ilavenil Soundharrajan , Bader O. Almutairi , Kathiravan Muthu Kumaradoss , Jesu Arockiaraj

Salmonellosis, a common foodborne illness caused by Salmonella spp., affects millions of people worldwide, and contamination of chicken meat and processing equipment poses a significant problem to the food industry. Quorum sensing inhibitors (QSIs) have been used to reduce biofilm formation by pathogens such as Salmonella enterica serovar Typhi (S. typhi), hence improving food safety. Given the concerns highlighted by regulatory organizations about the safety of traditional preservatives at high doses, there is an urgent need for alternatives. Chalcone derivatives have shown promise as safer preservatives, and this study looks into the anti-quorum sensing and preservative capabilities of DC05 (1-(5-chlorothiophen-2-yl)-5-[4-(dimethylamino)phenyl]prop-2,4-en-1-one) against S. typhi. DC05 showed antibacterial and antibiofilm activity at 80 μM and 20 μM respectively, significantly lowering exopolysaccharide (EPS) synthesis and downregulating important quorum-sensing genes such as luxS, lsrB, rpoS, and sdiA. It also reduced bacterial swimming and swarming motility (>0.35 cm). When mixed with sodium nitrate (NaNO₃), DC05 increased the shelf life of chicken meat significantly compared to the control group by suppressing bacterial growth (<8.50 log CFU/g), maintaining nutritional quality as seen in reduced moisture quantity (<58%), higher protein content (>20%) and carbohydrate content (>5%), lowering biogenic amine such as putrescine (<70 mg/kg) and cadaverine (<15 mg/kg) production, and improving overall acceptability score (>6 points). The results imply that DC05 has QSI action and can improve the performance of conventional preservatives at lower doses, hence extending the freshness and safety of poultry meat.

{"title":"Chalcone derivative enhance poultry meat preservation through quorum sensing inhibition against Salmonella (Salmonella enterica serovar Typhi) contamination","authors":"S.P. Ramya Ranjan Nayak , Pratik Pohokar , Anamika Das , L.S. Dhivya , Mukesh Pasupuleti , Ilavenil Soundharrajan , Bader O. Almutairi , Kathiravan Muthu Kumaradoss , Jesu Arockiaraj","doi":"10.1016/j.foodcont.2025.111155","DOIUrl":"10.1016/j.foodcont.2025.111155","url":null,"abstract":"<div><div>Salmonellosis, a common foodborne illness caused by <em>Salmonella</em> spp., affects millions of people worldwide, and contamination of chicken meat and processing equipment poses a significant problem to the food industry. Quorum sensing inhibitors (QSIs) have been used to reduce biofilm formation by pathogens such as <em>Salmonella enterica serovar Typhi</em> (<em>S. typhi</em>), hence improving food safety. Given the concerns highlighted by regulatory organizations about the safety of traditional preservatives at high doses, there is an urgent need for alternatives. Chalcone derivatives have shown promise as safer preservatives, and this study looks into the anti-quorum sensing and preservative capabilities of DC05 (1-(5-chlorothiophen-2-yl)-5-[4-(dimethylamino)phenyl]prop-2,4-en-1-one) against <em>S. typhi</em>. DC05 showed antibacterial and antibiofilm activity at 80 μM and 20 μM respectively, significantly lowering exopolysaccharide (EPS) synthesis and downregulating important quorum-sensing genes such as <em>luxS</em>, <em>lsrB</em>, <em>rpoS</em>, and <em>sdiA</em>. It also reduced bacterial swimming and swarming motility (>0.35 cm). When mixed with sodium nitrate (NaNO<sub>3</sub>), DC05 increased the shelf life of chicken meat significantly compared to the control group by suppressing bacterial growth (<8.50 log CFU/g), maintaining nutritional quality as seen in reduced moisture quantity (<58%), higher protein content (>20%) and carbohydrate content (>5%), lowering biogenic amine such as putrescine (<70 mg/kg) and cadaverine (<15 mg/kg) production, and improving overall acceptability score (>6 points). The results imply that DC05 has QSI action and can improve the performance of conventional preservatives at lower doses, hence extending the freshness and safety of poultry meat.</div></div>","PeriodicalId":319,"journal":{"name":"Food Control","volume":"171 ","pages":"Article 111155"},"PeriodicalIF":5.6,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143156767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel approach using UV-C light assisted by mild heat and natamycin as a hurdle strategy to preserve juice from non-commercial-grade apples

IF 5.6 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Control

Pub Date : 2025-01-12 DOI: 10.1016/j.foodcont.2025.111147

María de los Angeles Casco, Laura Schelegueda, Antonella Andreone, Marcela Schenk, Carmen A. Campos, Sandra N. Guerrero

The goal of this study was to investigate the effectiveness of using single UV-C light (1271 mJ/cm²) or assisted by mild heat (50 °C, UV-C/H) and the addition of 30 or 45 ppm of natamycin (Na) as emerging alternatives to preserve non-commercial-grade apple juice. Challenge tests using cocktails of E.coli (ECC), Salmonella (SC), and yeasts (YC) were performed, and Weibull and biphasic models were used to characterise microbial inactivation. Furthermore, juice sensory and non-sensory attributes and microbial and physicochemical stability during storage (23 days, 7 °C) were assessed. UV-C/H induced more significant inactivation in the bacterial cocktails These models distinctively explained microbial inactivation. During storage, UV-C/H prevented ECC and SC recovery, while both concentrations of Na increased YC inactivation and effectively prevented its recovery in the apple juice during the whole storage. A combined treatment of UV-C/H+30Na was proposed for subsequent analyses. Several physicochemical parameters, including pH, turbidity, soluble solids, 5-HMF content, D-fructose, total antioxidant activity (TAA) determined by DPPH (2,2-diphenyl-1-picrylhydrazyl), and total polyphenols remained unchanged in UV-C/H+30Na-treated juice However, it increased the total antioxidant activity by ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)) Principal component analysis (PCA) showed that, during storage, the apple juice sample without any treatment (C) was positively associated with increases in native microbiota, turbidity and opacity In contrast, the UV-C/H+30Na-treated juice showed complete microbiota inactivation and increased values of TAA_ABTS and flavonoids. The addition of natamycin to the apple juice preserved by UV-C light assisted by mild heat resulted in no significant differences in sensory and non-sensory attributes as judged by a wide group of consumers, who appreciated the samples.

{"title":"A novel approach using UV-C light assisted by mild heat and natamycin as a hurdle strategy to preserve juice from non-commercial-grade apples","authors":"María de los Angeles Casco, Laura Schelegueda, Antonella Andreone, Marcela Schenk, Carmen A. Campos, Sandra N. Guerrero","doi":"10.1016/j.foodcont.2025.111147","DOIUrl":"10.1016/j.foodcont.2025.111147","url":null,"abstract":"<div><div>The goal of this study was to investigate the effectiveness of using single UV-C light (1271 mJ/cm<sup>2</sup>) or assisted by mild heat (50 °C, UV-C/H) and the addition of 30 or 45 ppm of natamycin (Na) as emerging alternatives to preserve non-commercial-grade apple juice. Challenge tests using cocktails of <em>E.coli</em> (ECC), <em>Salmonella</em> (SC), and yeasts (YC) were performed, and Weibull and biphasic models were used to characterise microbial inactivation. Furthermore, juice sensory and non-sensory attributes and microbial and physicochemical stability during storage (23 days, 7 °C) were assessed. UV-C/H induced more significant inactivation in the bacterial cocktails These models distinctively explained microbial inactivation. During storage, UV-C/H prevented ECC and SC recovery, while both concentrations of Na increased YC inactivation and effectively prevented its recovery in the apple juice during the whole storage. A combined treatment of UV-C/H+30Na was proposed for subsequent analyses. Several physicochemical parameters, including pH, turbidity, soluble solids, 5-HMF content, D-fructose, total antioxidant activity (TAA) determined by DPPH (2,2-diphenyl-1-picrylhydrazyl), and total polyphenols remained unchanged in UV-C/H+30Na-treated juice However, it increased the total antioxidant activity by ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)) Principal component analysis (PCA) showed that, during storage, the apple juice sample without any treatment (C) was positively associated with increases in native microbiota, turbidity and opacity In contrast, the UV-C/H+30Na-treated juice showed complete microbiota inactivation and increased values of TAA<sub>ABTS</sub> and flavonoids. The addition of natamycin to the apple juice preserved by UV-C light assisted by mild heat resulted in no significant differences in sensory and non-sensory attributes as judged by a wide group of consumers, who appreciated the samples.</div></div>","PeriodicalId":319,"journal":{"name":"Food Control","volume":"171 ","pages":"Article 111147"},"PeriodicalIF":5.6,"publicationDate":"2025-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143156764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Simultaneous broad-spectrum detection of over 50 mycotoxins in maize silage via liquid chromatography-tandem mass spectrometry: Method development and application

IF 5.6 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Control

Pub Date : 2025-01-11 DOI: 10.1016/j.foodcont.2025.111143

Dawei Zhang , Bo Sun , Yujing Luan , Xueting Zhou , Jin Huang , Yucui Sun , Shoushuai Feng , Hailin Yang

For a comprehensive assessment of mycotoxin contamination in maize silage, a novel method using high-performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) that can simultaneously detect 53 mycotoxins in maize silage was developed. The method streamlines the extraction and purification processes with a simplified dilute-and-shoot sample pretreatment step. After extraction with acetonitrile/water/formic acid (70:30:0.1, v/v/v) followed by the LC-MS/MS detection. Furthermore, isotope internal standards were employed to eliminate matrix effects. The method was validated by linearity, matrix effects, selectivity, recovery, repeatability, limits of detection (LOD), limits of quantification (LOQ) and measurement uncertainties. The LODs ranged from 0.3 to 162.2 μg/kg and the LOQs from 0.9 to 535.4 μg/kg. The method developed enables the effective quantitative analysis of 51 out of 53 mycotoxins, with mean recoveries for 51 qualitatively determinable analytes applicable to this method ranging from 33.20% to 119.50% and relative standard deviations of less than 13.33%; all 53 mycotoxins could be qualitatively analyzed, and the method was successfully applied for analyzing 63 maize silage samples from China. Most of the mycotoxins detected were attributed to the Fusarium species. highlighting the prevalence of this genus in maize silage contamination. The developed LC-MS/MS method provides an excellent analytical performance for detecting multiple mycotoxins, marking it as the first report of such broad-spectrum detection in silage, and offers an effective tool for the accurate assessment of the true mycotoxin content in maize silage and the precise evaluation of feed quality.

{"title":"Simultaneous broad-spectrum detection of over 50 mycotoxins in maize silage via liquid chromatography-tandem mass spectrometry: Method development and application","authors":"Dawei Zhang , Bo Sun , Yujing Luan , Xueting Zhou , Jin Huang , Yucui Sun , Shoushuai Feng , Hailin Yang","doi":"10.1016/j.foodcont.2025.111143","DOIUrl":"10.1016/j.foodcont.2025.111143","url":null,"abstract":"<div><div>For a comprehensive assessment of mycotoxin contamination in maize silage, a novel method using high-performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) that can simultaneously detect 53 mycotoxins in maize silage was developed. The method streamlines the extraction and purification processes with a simplified dilute-and-shoot sample pretreatment step. After extraction with acetonitrile/water/formic acid (70:30:0.1, v/v/v) followed by the LC-MS/MS detection. Furthermore, isotope internal standards were employed to eliminate matrix effects. The method was validated by linearity, matrix effects, selectivity, recovery, repeatability, limits of detection (LOD), limits of quantification (LOQ) and measurement uncertainties. The LODs ranged from 0.3 to 162.2 μg/kg and the LOQs from 0.9 to 535.4 μg/kg. The method developed enables the effective quantitative analysis of 51 out of 53 mycotoxins, with mean recoveries for 51 qualitatively determinable analytes applicable to this method ranging from 33.20% to 119.50% and relative standard deviations of less than 13.33%; all 53 mycotoxins could be qualitatively analyzed, and the method was successfully applied for analyzing 63 maize silage samples from China. Most of the mycotoxins detected were attributed to the Fusarium species. highlighting the prevalence of this genus in maize silage contamination. The developed LC-MS/MS method provides an excellent analytical performance for detecting multiple mycotoxins, marking it as the first report of such broad-spectrum detection in silage, and offers an effective tool for the accurate assessment of the true mycotoxin content in maize silage and the precise evaluation of feed quality.</div></div>","PeriodicalId":319,"journal":{"name":"Food Control","volume":"172 ","pages":"Article 111143"},"PeriodicalIF":5.6,"publicationDate":"2025-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143139591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antibacterial mechanism of chitooligosaccharides against specific spoilage organisms in chilled processed fish paste products

IF 5.6 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Control

Pub Date : 2025-01-10 DOI: 10.1016/j.foodcont.2025.111148

Lin Zhang , Yue Wang , Siqi Ling , Mingying Yuan , Qinxiu Sun , Xiuping Dong

This study investigates the application and mechanism of chitooligosaccharides (COS) in inhibiting the spoilage caused by dominant spoilage bacteria in fish paste products (PFP). The minimum inhibitory concentration (MIC) of COS against dominant spoilage bacteria, including Brochothrix thermosphacta, Carnobacterium maltaromaticum, and Pseudomonas putida, was 25 mg/mL. The research demonstrates that COS disrupts bacterial cell membranes, leading to leakage of intracellular contents. Scanning electron microscopy revealed morphological changes in COS-treated bacteria, including deformation and fragmentation. Zeta-potential measurements showed that COS neutralizes the negative charge on bacterial surfaces, altering membrane permeability. Propidium iodide staining indicated that higher concentrations of COS resulted in increased membrane damage. Furthermore, COS treatment significantly enhanced protein release from the bacteria, indicating compromised membrane integrity. These findings underscore the effectiveness of COS in inhibiting spoilage bacteria through multiple mechanisms, thereby improving the preservation of PFP.

{"title":"Antibacterial mechanism of chitooligosaccharides against specific spoilage organisms in chilled processed fish paste products","authors":"Lin Zhang , Yue Wang , Siqi Ling , Mingying Yuan , Qinxiu Sun , Xiuping Dong","doi":"10.1016/j.foodcont.2025.111148","DOIUrl":"10.1016/j.foodcont.2025.111148","url":null,"abstract":"<div><div>This study investigates the application and mechanism of chitooligosaccharides (COS) in inhibiting the spoilage caused by dominant spoilage bacteria in fish paste products (PFP). The minimum inhibitory concentration (MIC) of COS against dominant spoilage bacteria, including <em>Brochothrix thermosphacta</em>, <em>Carnobacterium maltaromaticum</em>, and <em>Pseudomonas putida</em>, was 25 mg/mL. The research demonstrates that COS disrupts bacterial cell membranes, leading to leakage of intracellular contents. Scanning electron microscopy revealed morphological changes in COS-treated bacteria, including deformation and fragmentation. Zeta-potential measurements showed that COS neutralizes the negative charge on bacterial surfaces, altering membrane permeability. Propidium iodide staining indicated that higher concentrations of COS resulted in increased membrane damage. Furthermore, COS treatment significantly enhanced protein release from the bacteria, indicating compromised membrane integrity. These findings underscore the effectiveness of COS in inhibiting spoilage bacteria through multiple mechanisms, thereby improving the preservation of PFP.</div></div>","PeriodicalId":319,"journal":{"name":"Food Control","volume":"172 ","pages":"Article 111148"},"PeriodicalIF":5.6,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143139646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Construction of linear causal-regulated and autocatalysis-driven DNA circuits for highly sensitive and specific detection of Salmonella Typhimurium

IF 5.6 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Control

Pub Date : 2025-01-10 DOI: 10.1016/j.foodcont.2025.111153

Xinru Ren , Weiqing Sun , Bowen Li , Yijing Xiao , Xudong Yue , Meili Yang , Qing-ao Pang , Rui Zhu , Zhiqiang Guo , Haiping Zhang , Yu Wang , Su Liu , Jiadong Huang

The detection of pathogenic bacteria plays a crucial role in ensuring food safety and public health. The integration of isothermal amplification technology with the clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated proteins (Cas) system offers a versatile approach for detecting foodborne pathogens. However, this method is often constrained by the incompatibility of the reaction systems and the lack of control over CRISPR/Cas activity. Here, we demonstrated a fluorescence biosensing strategy based on linear causal-regulated and autocatalysis-driven DNA circuits (termed LC@ADC) for highly sensitive and specific detection of Salmonella Typhimurium (S. Typhimurium). The approach of combining hybridization chain reaction (HCR) with DNAzyme-driven CRISPR/Cas12a activation was proposed for the first time, enabling not only one-step cascade signal amplification but also controllable DNA circuits. The LC@ADC strategy is capable of quantifying concentrations of S. Typhimurium ranging from 10 to 1 × 10⁵ CFU/mL, with a detection limit as low as 6.53 CFU/mL. Furthermore, the LC@ADC strategy has been successfully employed for the detection of S. Typhimurium in samples of orange juice, chicken, and lake water. Therefore, this strategy provides a novel approach for the high-performance detection of pathogenic bacteria.

{"title":"Construction of linear causal-regulated and autocatalysis-driven DNA circuits for highly sensitive and specific detection of Salmonella Typhimurium","authors":"Xinru Ren , Weiqing Sun , Bowen Li , Yijing Xiao , Xudong Yue , Meili Yang , Qing-ao Pang , Rui Zhu , Zhiqiang Guo , Haiping Zhang , Yu Wang , Su Liu , Jiadong Huang","doi":"10.1016/j.foodcont.2025.111153","DOIUrl":"10.1016/j.foodcont.2025.111153","url":null,"abstract":"<div><div>The detection of pathogenic bacteria plays a crucial role in ensuring food safety and public health. The integration of isothermal amplification technology with the clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated proteins (Cas) system offers a versatile approach for detecting foodborne pathogens. However, this method is often constrained by the incompatibility of the reaction systems and the lack of control over CRISPR/Cas activity. Here, we demonstrated a fluorescence biosensing strategy based on linear causal-regulated and autocatalysis-driven DNA circuits (termed LC@ADC) for highly sensitive and specific detection of <em>Salmonella T</em><em>yphimurium</em> (<em>S. Typhimurium</em>). The approach of combining hybridization chain reaction (HCR) with DNAzyme-driven CRISPR/Cas12a activation was proposed for the first time, enabling not only one-step cascade signal amplification but also controllable DNA circuits. The LC@ADC strategy is capable of quantifying concentrations of <em>S. Typhimurium</em> ranging from 10 to 1 × 10<sup>5</sup> CFU/mL, with a detection limit as low as 6.53 CFU/mL. Furthermore, the LC@ADC strategy has been successfully employed for the detection of <em>S. Typhimurium</em> in samples of orange juice, chicken, and lake water. Therefore, this strategy provides a novel approach for the high-performance detection of pathogenic bacteria.</div></div>","PeriodicalId":319,"journal":{"name":"Food Control","volume":"172 ","pages":"Article 111153"},"PeriodicalIF":5.6,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143139645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel insights into the physiological and molecular responses of Pseudomonas fragi to CO2 for high-oxygen packaged meat preservation

IF 5.6 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Control

Pub Date : 2025-01-10 DOI: 10.1016/j.foodcont.2025.111149

Jun Yang , Yanwei Mao , Pengcheng Dong , Rongrong Liang , Lixian Zhu , Xin Luo , Yimin Zhang , Xiaoyin Yang

The CO₂ in high-oxygen modified atmosphere packaging (HiOx-MAP) could efficiently inhibit the growth of dominant Pseudomonas fragi in meat. However, little detailed information is available about the changes in P. fragi intracellular physiological properties and corresponding proteome and gene regulation in response to CO₂. This study aimed to elucidate the molecular antibacterial mechanism of CO₂ against Pseudomonas fragi held under both HiOx-MAP systems with various CO₂ concentrations (50% O₂/40% CO₂/10% N₂; 50% O₂/50% N₂). P. fragi induced meat spoilage (bacterial counts, pH, and total volatile basic nitrogen), and its physiological characteristics (intracellular pH (pH_i), adenosine triphosphate (ATP) and reactive oxygen species (ROS) levels, and motility), combined with bacterial proteome and targeted gene expression were investigated during chilled storage. Results showed that CO₂ suppressed P. fragi growth and spoilage potential by decreasing ATP contents and motility ability, associated with down-regulated protein and gene expression of glycolysis, flagellar assembly, and chemotaxis (e.g., aldehyde dehydrogenase (aldB), flagellar protein (fliG and motA)). CO₂ also induced oxidative stress via elevated ROS levels at the initial stage, and damaged cell membrane stability and genetic information processing by down-regulating outer membrane porin (oprD) and ribonuclease HI (rnhB) expression. However, CO₂ activated P. fragi citrate cycle to supply energy, and enhanced intracellular redox and pH homeostasis by up-regulating protein and gene expression of glutathione peroxidase, ATPase, and arginine deiminase. These findings underpin a significant advancement in understanding CO₂-bacteriostatic theory for meat packaging preservation.

{"title":"Novel insights into the physiological and molecular responses of Pseudomonas fragi to CO2 for high-oxygen packaged meat preservation","authors":"Jun Yang , Yanwei Mao , Pengcheng Dong , Rongrong Liang , Lixian Zhu , Xin Luo , Yimin Zhang , Xiaoyin Yang","doi":"10.1016/j.foodcont.2025.111149","DOIUrl":"10.1016/j.foodcont.2025.111149","url":null,"abstract":"<div><div>The CO<sub>2</sub> in high-oxygen modified atmosphere packaging (HiOx-MAP) could efficiently inhibit the growth of dominant <em>Pseudomonas fragi</em> in meat. However, little detailed information is available about the changes in <em>P. fragi</em> intracellular physiological properties and corresponding proteome and gene regulation in response to CO<sub>2</sub>. This study aimed to elucidate the molecular antibacterial mechanism of CO<sub>2</sub> against <em>Pseudomonas fragi</em> held under both HiOx-MAP systems with various CO<sub>2</sub> concentrations (50% O<sub>2</sub>/40% CO<sub>2</sub>/10% N<sub>2</sub>; 50% O<sub>2</sub>/50% N<sub>2</sub>). <em>P. fragi</em> induced meat spoilage (bacterial counts, pH, and total volatile basic nitrogen), and its physiological characteristics (intracellular pH (pH<sub>i</sub>), adenosine triphosphate (ATP) and reactive oxygen species (ROS) levels, and motility), combined with bacterial proteome and targeted gene expression were investigated during chilled storage. Results showed that CO<sub>2</sub> suppressed <em>P. fragi</em> growth and spoilage potential by decreasing ATP contents and motility ability, associated with down-regulated protein and gene expression of glycolysis, flagellar assembly, and chemotaxis (e.g., aldehyde dehydrogenase (<em>aldB</em>), flagellar protein (<em>fliG</em> and <em>motA</em>)). CO<sub>2</sub> also induced oxidative stress via elevated ROS levels at the initial stage, and damaged cell membrane stability and genetic information processing by down-regulating outer membrane porin (<em>oprD</em>) and ribonuclease HI (<em>rnhB</em>) expression. However, CO<sub>2</sub> activated <em>P. fragi</em> citrate cycle to supply energy, and enhanced intracellular redox and pH homeostasis by up-regulating protein and gene expression of glutathione peroxidase, ATPase, and arginine deiminase. These findings underpin a significant advancement in understanding CO<sub>2</sub>-bacteriostatic theory for meat packaging preservation.</div></div>","PeriodicalId":319,"journal":{"name":"Food Control","volume":"171 ","pages":"Article 111149"},"PeriodicalIF":5.6,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143100511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Occurrence, processing factors, and dietary risk assessment of multi-pesticides in cowpea

IF 5.6 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Control

Pub Date : 2025-01-09 DOI: 10.1016/j.foodcont.2025.111145

Kunyu Wang , Mingyu Liu , Tongtong Wang , Han Xu , Xiaoli Liu , Peng Jiang , Xiaoming Li , Hui Li , Jun Yang , Jiyun Nie

Cowpea is an important legume and vegetable crop in China. However, there have been few reports on the monitoring of pesticide residues in cowpea and the effect of household washing on pesticide residues in cowpea samples. In this study, 33 pesticide residues were analyzed in cowpea. A total of 21 pesticide residues were detected in all samples, of which 12 were not approved for use in cowpea. Among these pesticides, the residue of chlorpyrifos in one sample and thiamethoxam in five samples exceeded the China national maximum residue limits. Multiple residues were recorded in 79.3% of the positive cowpea samples. The results indicated misuse of unregistered pesticides, irregular use of pesticides, and multiple pesticide residues in cowpea samples studied. We also compared the effectiveness of water soaking and detergent solution soaking to remove the pesticide residues. The results showed that detergent solution soaking (average processing factor PF of 0.22) showed better performance than water soaking (average PF of 0.47). Moreover, the removal of dinotefuran, dimethoate, and indoxacarb pesticides was lower than 50% by water soaking and detergent soaking, which may be due to the fact that some of the pesticides are systemic and permeable. Finally, a risk assessment of pesticide residues was carried out on cowpea samples. The results showed that the indices (%ADI and %ARfD) of the chronic and acute dietary intake risks of pesticide residues in cowpea samples were all lower than 100% and do not pose a health risk to cowpea consumers. We also found that children and females have higher health risks than adults and males, which is related to the body weight of different populations and cowpea intake.

{"title":"Occurrence, processing factors, and dietary risk assessment of multi-pesticides in cowpea","authors":"Kunyu Wang , Mingyu Liu , Tongtong Wang , Han Xu , Xiaoli Liu , Peng Jiang , Xiaoming Li , Hui Li , Jun Yang , Jiyun Nie","doi":"10.1016/j.foodcont.2025.111145","DOIUrl":"10.1016/j.foodcont.2025.111145","url":null,"abstract":"<div><div>Cowpea is an important legume and vegetable crop in China. However, there have been few reports on the monitoring of pesticide residues in cowpea and the effect of household washing on pesticide residues in cowpea samples. In this study, 33 pesticide residues were analyzed in cowpea. A total of 21 pesticide residues were detected in all samples, of which 12 were not approved for use in cowpea. Among these pesticides, the residue of chlorpyrifos in one sample and thiamethoxam in five samples exceeded the China national maximum residue limits. Multiple residues were recorded in 79.3% of the positive cowpea samples. The results indicated misuse of unregistered pesticides, irregular use of pesticides, and multiple pesticide residues in cowpea samples studied. We also compared the effectiveness of water soaking and detergent solution soaking to remove the pesticide residues. The results showed that detergent solution soaking (average processing factor <em>PF</em> of 0.22) showed better performance than water soaking (average <em>PF</em> of 0.47). Moreover, the removal of dinotefuran, dimethoate, and indoxacarb pesticides was lower than 50% by water soaking and detergent soaking, which may be due to the fact that some of the pesticides are systemic and permeable. Finally, a risk assessment of pesticide residues was carried out on cowpea samples. The results showed that the indices (<em>%ADI</em> and <em>%ARfD</em>) of the chronic and acute dietary intake risks of pesticide residues in cowpea samples were all lower than 100% and do not pose a health risk to cowpea consumers. We also found that children and females have higher health risks than adults and males, which is related to the body weight of different populations and cowpea intake.</div></div>","PeriodicalId":319,"journal":{"name":"Food Control","volume":"171 ","pages":"Article 111145"},"PeriodicalIF":5.6,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143157380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrating magnetic-plasmonic and membrane-like nanotags for the sensitive and reliable detection of aflatoxin B1 in foodstuffs

IF 5.6 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Control

Pub Date : 2025-01-09 DOI: 10.1016/j.foodcont.2025.111144

Di Zhang , Wenhao Yang , Xin Li , Xiaobo Zou , Lidan Niu , Shipeng Gao

Ultrasensitive and reliable detection of aflatoxin B₁ (AFB₁), one of the most toxic mycotoxins and potent carcinogens in contaminated foodstuffs, is highly desired to prevent its health threat. Herein, a nanoassembly-type surface-enhanced Raman scattering (SERS) aptasensor is proposed for sensitive and reliable AFB₁ detection in foodstuffs. The nanoassembly comprises a cDNA-labeled graphene oxide (GO)-based membrane-like SERS nanotag, formed by assembling a dense layer of AuNPs onto the GO nanosheet with interparticle nanogaps of less than 3 nm for featuring signal amplification. Additionally, it includes an aptamer-conjugated core (Fe₃O₄ nanoparticles)-shell (Ag) magnetic-plasmonic nanostructure (Fe₃O₄@Ag) designed to mitigate matrix interference and offer an additional signal improvement through tuning the morphology of AgNPs. The recognition binding of aptamers with analytes causes the detachment of magnetic probes to promote a reduction of Raman intensity as AFB₁ concentration increases. This aptasensor exhibits favorable sensitivity (detection limit of 2.31 pg/mL), detection adaptability (linear range of 10⁻² ng/mL to 10³ ng/mL), real sample reliability (vinegar, milk, and bran barley), reproducibility (relative standard deviations of 1.19%–2.52%), and storage stability (>30 days), benefiting from signal amplification endowed by membrane-like nanotags and magnetic separation in minimizing matrix effects. Our method demonstrates enormous potential for fulfilling the requirements of determining AFB₁ in real samples with satisfactory sensitivity and reliability.

{"title":"Integrating magnetic-plasmonic and membrane-like nanotags for the sensitive and reliable detection of aflatoxin B1 in foodstuffs","authors":"Di Zhang , Wenhao Yang , Xin Li , Xiaobo Zou , Lidan Niu , Shipeng Gao","doi":"10.1016/j.foodcont.2025.111144","DOIUrl":"10.1016/j.foodcont.2025.111144","url":null,"abstract":"<div><div>Ultrasensitive and reliable detection of aflatoxin B<sub>1</sub> (AFB<sub>1</sub>), one of the most toxic mycotoxins and potent carcinogens in contaminated foodstuffs, is highly desired to prevent its health threat. Herein, a nanoassembly-type surface-enhanced Raman scattering (SERS) aptasensor is proposed for sensitive and reliable AFB<sub>1</sub> detection in foodstuffs. The nanoassembly comprises a cDNA-labeled graphene oxide (GO)-based membrane-like SERS nanotag, formed by assembling a dense layer of AuNPs onto the GO nanosheet with interparticle nanogaps of less than 3 nm for featuring signal amplification. Additionally, it includes an aptamer-conjugated core (Fe<sub>3</sub>O<sub>4</sub> nanoparticles)-shell (Ag) magnetic-plasmonic nanostructure (Fe<sub>3</sub>O<sub>4</sub>@Ag) designed to mitigate matrix interference and offer an additional signal improvement through tuning the morphology of AgNPs. The recognition binding of aptamers with analytes causes the detachment of magnetic probes to promote a reduction of Raman intensity as AFB<sub>1</sub> concentration increases. This aptasensor exhibits favorable sensitivity (detection limit of 2.31 pg/mL), detection adaptability (linear range of 10<sup>−2</sup> ng/mL to 10<sup>3</sup> ng/mL), real sample reliability (vinegar, milk, and bran barley), reproducibility (relative standard deviations of 1.19%–2.52%), and storage stability (>30 days), benefiting from signal amplification endowed by membrane-like nanotags and magnetic separation in minimizing matrix effects. Our method demonstrates enormous potential for fulfilling the requirements of determining AFB<sub>1</sub> in real samples with satisfactory sensitivity and reliability.</div></div>","PeriodicalId":319,"journal":{"name":"Food Control","volume":"171 ","pages":"Article 111144"},"PeriodicalIF":5.6,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143156765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Heavy metals in marketed hake and tuna highlight pollution hotspots in old continent waters

IF 5.6 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Control

Pub Date : 2025-01-09 DOI: 10.1016/j.foodcont.2025.111140

Carlos Valiente-Diaz , Juan L. López , Alba Ardura , Carmen Blanco-Fernandez , Marlene Bartolomé , Celia Delgado , Verónica Soto-López , Deva Menéndez-Teleña , Gonzalo Machado-Schiaffino , Eva Garcia-Vazquez

Heavy metals are legacy pollutants that can accumulate in marine fish. For their toxicity they represent a potential risk for seafood consumers, especially in global seafood markets that import fish from polluted regions. Here we analyzed heavy metal contents in local and imported hake and tuna species marketed in Spain (N = 123). One individual exceeding the safety level according to European regulations for Cd and two for Hg were found, being 4.1% of the analyzed fish. Hakes were more polluted by As and tunas by Hg. For higher risk indices Estimated Weekly Intake (EWI) and Allowable Limit of Consumption Rate, FAO 27 (Northeast Atlantic waters, Europe) and FAO 34 (central East Atlantic, West Africa) contained the most polluted fish, being FAO 27 a hotspot for As and FAO 34 for Hg. Possible sources of As, Cd and Hg in each region are discussed. For food safety, controlling seafood heavy metal content, preventing, and mitigating heavy metal pollution should be priority in these pollution hotspots.

{"title":"Heavy metals in marketed hake and tuna highlight pollution hotspots in old continent waters","authors":"Carlos Valiente-Diaz , Juan L. López , Alba Ardura , Carmen Blanco-Fernandez , Marlene Bartolomé , Celia Delgado , Verónica Soto-López , Deva Menéndez-Teleña , Gonzalo Machado-Schiaffino , Eva Garcia-Vazquez","doi":"10.1016/j.foodcont.2025.111140","DOIUrl":"10.1016/j.foodcont.2025.111140","url":null,"abstract":"<div><div>Heavy metals are legacy pollutants that can accumulate in marine fish. For their toxicity they represent a potential risk for seafood consumers, especially in global seafood markets that import fish from polluted regions. Here we analyzed heavy metal contents in local and imported hake and tuna species marketed in Spain (N = 123). One individual exceeding the safety level according to European regulations for Cd and two for Hg were found, being 4.1% of the analyzed fish. Hakes were more polluted by As and tunas by Hg. For higher risk indices Estimated Weekly Intake (EWI) and Allowable Limit of Consumption Rate, FAO 27 (Northeast Atlantic waters, Europe) and FAO 34 (central East Atlantic, West Africa) contained the most polluted fish, being FAO 27 a hotspot for As and FAO 34 for Hg. Possible sources of As, Cd and Hg in each region are discussed. For food safety, controlling seafood heavy metal content, preventing, and mitigating heavy metal pollution should be priority in these pollution hotspots.</div></div>","PeriodicalId":319,"journal":{"name":"Food Control","volume":"171 ","pages":"Article 111140"},"PeriodicalIF":5.6,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143100512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quantification of three furfurals in food using a triazine-based hydrazine derivatization reagent by MALDI mass spectrometry

IF 5.6 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Control

Pub Date : 2025-01-08 DOI: 10.1016/j.foodcont.2025.111146

Zhang-Na Qin , Zhen-Peng Zhen , Yu-Feng Gao

In this study, a high-throughput matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) method was developed for detecting three furfurals (furfural, 5-methylfurfural, and 5-hydroxymethylfurfural) in food. The derivatization of furfurals with 4-dimethylamino-6-(4-methoxy-1-naphthyl)-1,3,5-triazine-2-hydrazine (DMNTH) resulted in a substantial increase in the m/z values of furfurals and an improvement in detection sensitivity. The compound 5-methoxymethylfurfural, a structural analogue of furfurals, was utilized as an internal standard to correct for fluctuations in mass spectrometry signals and matrix effects present in the real samples. The calibration curves exhibited good linearity in the furfural concentration range of 0.5–50 μg/mL, with correlation coefficients (R) exceeding 0.9996. The limits of quantification were in the range of 0.20–0.39 μg/mL. The furfural recoveries ranged from 89.2% to 116.2%, and the intra- and inter-day precisions were less than 10.8%, indicating high detection accuracy and precision. The developed method was successfully applied to the detection of furfurals in a variety of real samples, including roasted coffee beans, honey, and vinegar. The results demonstrated that the developed method was rapid and high-throughput, meeting the requirements for accurately detecting furfurals in various foods.

{"title":"Quantification of three furfurals in food using a triazine-based hydrazine derivatization reagent by MALDI mass spectrometry","authors":"Zhang-Na Qin , Zhen-Peng Zhen , Yu-Feng Gao","doi":"10.1016/j.foodcont.2025.111146","DOIUrl":"10.1016/j.foodcont.2025.111146","url":null,"abstract":"<div><div>In this study, a high-throughput matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) method was developed for detecting three furfurals (furfural, 5-methylfurfural, and 5-hydroxymethylfurfural) in food. The derivatization of furfurals with 4-dimethylamino-6-(4-methoxy-1-naphthyl)-1,3,5-triazine-2-hydrazine (DMNTH) resulted in a substantial increase in the <em>m/z</em> values of furfurals and an improvement in detection sensitivity. The compound 5-methoxymethylfurfural, a structural analogue of furfurals, was utilized as an internal standard to correct for fluctuations in mass spectrometry signals and matrix effects present in the real samples. The calibration curves exhibited good linearity in the furfural concentration range of 0.5–50 μg/mL, with correlation coefficients (R) exceeding 0.9996. The limits of quantification were in the range of 0.20–0.39 μg/mL. The furfural recoveries ranged from 89.2% to 116.2%, and the intra- and inter-day precisions were less than 10.8%, indicating high detection accuracy and precision. The developed method was successfully applied to the detection of furfurals in a variety of real samples, including roasted coffee beans, honey, and vinegar. The results demonstrated that the developed method was rapid and high-throughput, meeting the requirements for accurately detecting furfurals in various foods.</div></div>","PeriodicalId":319,"journal":{"name":"Food Control","volume":"171 ","pages":"Article 111146"},"PeriodicalIF":5.6,"publicationDate":"2025-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143157382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0