Pub Date : 2024-06-25DOI: 10.1016/j.foodcont.2024.110680
Jialin Wang , Yaqing Yang , Aaron Albert Aryee, Ningning Wang, Zhaohui Li
Excessive consumption of flour products containing excessive azodicarbonamide (ADA) has been associated with some adverse effects in humans. Thus, developing a facile method to monitor the amount of ADA in flour is extremely significant. Herein, a graphene quantum dot (GQD) sensitized upconversion nanoparticles (GQD-UCNPs) was designed to monitor the amount of ADA in flour with high specificity and sensitivity. Surface coating of UCNPs with GQD by coordination interaction significantly enhances the upconversion luminescence (UCL) intensity and the water solubility of UCNPs. Copper ions (Cu2+) can quench the UCL intensity of GQD-UCNPs through electron transfer processes. After introduction of glutathione (GSH), the UCL intensity of GQD-UCNPs gradually increases because of the interaction between GSH and Cu2+, accompanied by the generation of Cu+. The sulfydryl group of GSH can be oxidized to disulfide bond by ADA, which hinders the interaction between GSH and Cu2+, preventing the recovery of UCL intensity. Thus, the presence of ADA decreases the UCL intensity of GQD-UCNPs + Cu2+ + GSH system, allowing for the quantitative detection of ADA. The nanosensor realized a highly sensitive and specific ADA detection with a high detection limit down to 0.055 μM. Furthermore, excellent recovery rates in spiked real samples were obtained, indicating that the designed method possesses good prospects for practical application. Notably, this method broadens the application prospects of upconversion technology in the field of food safety.
{"title":"Graphene quantum dot-functionalized upconversion nanoparticles for highly sensitive detection of food contaminant azodicarbonamide","authors":"Jialin Wang , Yaqing Yang , Aaron Albert Aryee, Ningning Wang, Zhaohui Li","doi":"10.1016/j.foodcont.2024.110680","DOIUrl":"https://doi.org/10.1016/j.foodcont.2024.110680","url":null,"abstract":"<div><p>Excessive consumption of flour products containing excessive azodicarbonamide (ADA) has been associated with some adverse effects in humans. Thus, developing a facile method to monitor the amount of ADA in flour is extremely significant. Herein, a graphene quantum dot (GQD) sensitized upconversion nanoparticles (GQD-UCNPs) was designed to monitor the amount of ADA in flour with high specificity and sensitivity. Surface coating of UCNPs with GQD by coordination interaction significantly enhances the upconversion luminescence (UCL) intensity and the water solubility of UCNPs. Copper ions (Cu<sup>2+</sup>) can quench the UCL intensity of GQD-UCNPs through electron transfer processes. After introduction of glutathione (GSH), the UCL intensity of GQD-UCNPs gradually increases because of the interaction between GSH and Cu<sup>2+</sup>, accompanied by the generation of Cu<sup>+</sup>. The sulfydryl group of GSH can be oxidized to disulfide bond by ADA, which hinders the interaction between GSH and Cu<sup>2+</sup>, preventing the recovery of UCL intensity. Thus, the presence of ADA decreases the UCL intensity of GQD-UCNPs + Cu<sup>2+</sup> + GSH system, allowing for the quantitative detection of ADA. The nanosensor realized a highly sensitive and specific ADA detection with a high detection limit down to 0.055 μM. Furthermore, excellent recovery rates in spiked real samples were obtained, indicating that the designed method possesses good prospects for practical application. Notably, this method broadens the application prospects of upconversion technology in the field of food safety.</p></div>","PeriodicalId":319,"journal":{"name":"Food Control","volume":null,"pages":null},"PeriodicalIF":5.6,"publicationDate":"2024-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141484633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-25DOI: 10.1016/j.foodcont.2024.110675
Alice Giusti , Lara Tinacci , Enrica Ricci , Francesco Verdigi , Roberto Narducci , Lisa Vallone , Laura Gasperetti , Andrea Armani
Multi-species mushroom-based products (MPs) sold in Italy were authenticated by metabarcoding (MB). One degenerated primer pair was projected for the ITS-1 region amplification. The final data were interpreted using positive controls and extraction blanks. Sequences of macrofungi, microfungi and bacteria were detected in 100%, 90.9% and 77.3% of the MPs, respectively. Not edible and toxic macrofungi were also found, although in low sequence amount. The match between species declared on MPs label and species detected by MB was 59.1%. Cases of voluntary species substitution cannot be excluded. Methods to authenticate these products, still poorly investigated, should be further employed, and MB protocol should be standardized to be used in the context of both official control and companies’ self-control. Alternative approaches should be considered for canned and frozen MPs, in virtue of the observed DNA fragmentation.
{"title":"Next Generation Sequencing Technologies for the analysis of a poorly investigated foodstuff: mushroom products authentication by metabarcoding","authors":"Alice Giusti , Lara Tinacci , Enrica Ricci , Francesco Verdigi , Roberto Narducci , Lisa Vallone , Laura Gasperetti , Andrea Armani","doi":"10.1016/j.foodcont.2024.110675","DOIUrl":"https://doi.org/10.1016/j.foodcont.2024.110675","url":null,"abstract":"<div><p>Multi-species mushroom-based products (MPs) sold in Italy were authenticated by metabarcoding (MB). One degenerated primer pair was projected for the ITS-1 region amplification. The final data were interpreted using positive controls and extraction blanks. Sequences of macrofungi, microfungi and bacteria were detected in 100%, 90.9% and 77.3% of the MPs, respectively. Not edible and toxic macrofungi were also found, although in low sequence amount. The match between species declared on MPs label and species detected by MB was 59.1%. Cases of voluntary species substitution cannot be excluded. Methods to authenticate these products, still poorly investigated, should be further employed, and MB protocol should be standardized to be used in the context of both official control and companies’ self-control. Alternative approaches should be considered for canned and frozen MPs, in virtue of the observed DNA fragmentation.</p></div>","PeriodicalId":319,"journal":{"name":"Food Control","volume":null,"pages":null},"PeriodicalIF":5.6,"publicationDate":"2024-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141479870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-25DOI: 10.1016/j.foodcont.2024.110676
Le Zhao , Youyan Liang , Nuohan Zhang , Yanbin Chang , Meijuan Fan , Cong Wang , Beibei Li , Hongbo Wang , Yu Liu , Hongxiao Yu , Song Yang , Fuwei Xie , Cong Nie , Junwei Guo
In this study, we investigated the additive patterns observed in near-infrared (NIR) diffuse reflectance spectra within the context of food and pharmaceutical product formulation. Employing the Kubelka–Munk theory, we examined the linear correlation between spectra and concentration in polymer materials and tobacco powder samples. Our findings confirm the principle of spectral additivity in diffuse reflectance spectra, demonstrating a linear relationship when the sample scattering coefficient remains constant. Moreover, our results validate the feasibility of substituting actual mixed spectra with NIR additive spectra in tobacco leaf systems. This approach can potentially enhance formulation design, thereby improving efficiency and accuracy while expanding the scope and combination of formulation materials. Furthermore, this study offers a rapid, information-rich, and environmentally friendly alternative to traditional methods, with significant implications for the future of product formulation.
{"title":"Additive patterns in near-infrared diffuse reflectance spectra: Implications for product formulation and analysis","authors":"Le Zhao , Youyan Liang , Nuohan Zhang , Yanbin Chang , Meijuan Fan , Cong Wang , Beibei Li , Hongbo Wang , Yu Liu , Hongxiao Yu , Song Yang , Fuwei Xie , Cong Nie , Junwei Guo","doi":"10.1016/j.foodcont.2024.110676","DOIUrl":"https://doi.org/10.1016/j.foodcont.2024.110676","url":null,"abstract":"<div><p>In this study, we investigated the additive patterns observed in near-infrared (NIR) diffuse reflectance spectra within the context of food and pharmaceutical product formulation. Employing the Kubelka–Munk theory, we examined the linear correlation between spectra and concentration in polymer materials and tobacco powder samples. Our findings confirm the principle of spectral additivity in diffuse reflectance spectra, demonstrating a linear relationship when the sample scattering coefficient remains constant. Moreover, our results validate the feasibility of substituting actual mixed spectra with NIR additive spectra in tobacco leaf systems. This approach can potentially enhance formulation design, thereby improving efficiency and accuracy while expanding the scope and combination of formulation materials. Furthermore, this study offers a rapid, information-rich, and environmentally friendly alternative to traditional methods, with significant implications for the future of product formulation.</p></div>","PeriodicalId":319,"journal":{"name":"Food Control","volume":null,"pages":null},"PeriodicalIF":5.6,"publicationDate":"2024-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141479855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-25DOI: 10.1016/j.foodcont.2024.110677
Anderson dos Santos Formiga, Vivaldo Silveira Júnior
During the cold chain, fruits are exposed to variations in temperature and relative humidity, which will contribute to reducing the quality and commercial value of these perishable products. Aiming to extend the shelf life, reduce losses and increase profits from the trade of climacteric fruits, this work estimated the commercial value of ‘Pedro Sato’ guavas treated or not with hydrogen peroxide during the cold chain and evaluated the feasibility of replacing the management system of stock F.I.F.O. (first In, first Out) by F.E.F.O. (first expired, first out) The commercial value of guavas was determined by combining the values of weight loss, tonality and firmness, which were estimated by semi-empirical models. The experimental data showed that peroxide positively influenced the quality of guavas, quality assessments demonstrated that weight loss was the attribute that limited the shelf life of guavas and flow simulations, along the cold chain, confirmed that the implementation of a F.E.F.O. management system can increase retailer's profits by up to 13 %.
{"title":"Effects of gaseous hydrogen peroxide on quality, shelf life and distribution management strategy F.E.F.O. (first expired, first out) of guavas cv. Pedro Sato","authors":"Anderson dos Santos Formiga, Vivaldo Silveira Júnior","doi":"10.1016/j.foodcont.2024.110677","DOIUrl":"https://doi.org/10.1016/j.foodcont.2024.110677","url":null,"abstract":"<div><p>During the cold chain, fruits are exposed to variations in temperature and relative humidity, which will contribute to reducing the quality and commercial value of these perishable products. Aiming to extend the shelf life, reduce losses and increase profits from the trade of climacteric fruits, this work estimated the commercial value of ‘Pedro Sato’ guavas treated or not with hydrogen peroxide during the cold chain and evaluated the feasibility of replacing the management system of stock F.I.F.O. (first In, first Out) by F.E.F.O. (first expired, first out) The commercial value of guavas was determined by combining the values of weight loss, tonality and firmness, which were estimated by semi-empirical models. The experimental data showed that peroxide positively influenced the quality of guavas, quality assessments demonstrated that weight loss was the attribute that limited the shelf life of guavas and flow simulations, along the cold chain, confirmed that the implementation of a F.E.F.O. management system can increase retailer's profits by up to 13 %.</p></div>","PeriodicalId":319,"journal":{"name":"Food Control","volume":null,"pages":null},"PeriodicalIF":5.6,"publicationDate":"2024-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141484624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-24DOI: 10.1016/j.foodcont.2024.110662
Determining the geographical origin of seafood is crucial for regulators and fishing industries who seek to prevent commercial fraud, enforce food safety regulations, and encourage high standards in sustainable fisheries management. The cockle, Cerastoderma edule (Linnaeus 1768), is a key species in estuarine ecosystems and is harvested all over Europe. Therefore, traceability tools using quick and inexpensive techniques to identify the origin of this bivalve are of paramount importance to support law enforcement. In this work, we explore the potential of using Geometric Morphometric (GM) methods to identify the geographical origin of cockle specimens. This method is based on landmarks identified in the shell to trace the origin of specimens obtained in nearby aquatic systems (from <35 km to <250 km distance). Specimens were collected in five aquatic systems (Ria de Aveiro, the Tagus and Sado estuaries, and the Albufeira and Óbidos coastal lagoons) in Portugal. Shells were digitalized and 16 landmarks were identified in each right valve and analyzed using Generalized Procrustes Superimposition. The discriminating power for traceability of 12 statistical and machine learning methods was assessed based on the corresponding shape variables, using R and Python (Linear Discriminant Analysis (LDA), Canonical Variable Analysis (CVA), Principal Component Analysis (PCA), Between-Group PCA (bgPCA), Partial Least Squares Discriminant (PLSD), Classification Regression Tree (CRT), Logistic Regression (LR), Random Forest (RF), Gradient Boosting (GB), K Nearest Neighbors (KNN), Support Vector Machines (SVM), Extending Gradient Boosting (XGBoost) and Neural Networks (NNET). LDA, CVA, SVM, and NNET demonstrated greater accuracy and a F1-score >80%, even with a small and unbalanced sample size. The highest percentage of correctly assigned individuals was obtained in the Tagus estuary (mean 89%) and in the Albufeira lagoon (mean 93%), which were also the systems with more specimens measured (174 and 59 respectively), whereas the worst results were obtained in the Sado estuary (50%, 56 specimens). In the Albufeira coastal lagoon, the best classification methods reached 100% correct classifications. It further highlights the importance of establishing statistical standards, such as the ones developed in the current work, to evaluate different methods, as small changes in the procedure may cause substantial differences in the results and conclusions. The revision of previous works (presented as a table) showed often >90% of correct classification in both bivalves and gastropods, highlighting the potential of the techniques for other mollusks. Our results support the use of GM based on landmarks as a reliable tool for bivalve's traceability, since it is a quick, simple and inexpensive approach. Further research should extend these findings to other species and other shape analysis techniques.
{"title":"Using shell shape analysis based on landmarks to trace the geographical origin of the common cockle (Cerastoderma edule)","authors":"","doi":"10.1016/j.foodcont.2024.110662","DOIUrl":"10.1016/j.foodcont.2024.110662","url":null,"abstract":"<div><p>Determining the geographical origin of seafood is crucial for regulators and fishing industries who seek to prevent commercial fraud, enforce food safety regulations, and encourage high standards in sustainable fisheries management. The cockle, <em>Cerastoderma edule</em> (Linnaeus 1768), is a key species in estuarine ecosystems and is harvested all over Europe. Therefore, traceability tools using quick and inexpensive techniques to identify the origin of this bivalve are of paramount importance to support law enforcement. In this work, we explore the potential of using Geometric Morphometric (GM) methods to identify the geographical origin of cockle specimens. This method is based on landmarks identified in the shell to trace the origin of specimens obtained in nearby aquatic systems (from <35 km to <250 km distance). Specimens were collected in five aquatic systems (Ria de Aveiro, the Tagus and Sado estuaries, and the Albufeira and Óbidos coastal lagoons) in Portugal. Shells were digitalized and 16 landmarks were identified in each right valve and analyzed using Generalized Procrustes Superimposition. The discriminating power for traceability of 12 statistical and machine learning methods was assessed based on the corresponding shape variables, using R and Python (Linear Discriminant Analysis (LDA), Canonical Variable Analysis (CVA), Principal Component Analysis (PCA), Between-Group PCA (bgPCA), Partial Least Squares Discriminant (PLSD), Classification Regression Tree (CRT), Logistic Regression (LR), Random Forest (RF), Gradient Boosting (GB), K Nearest Neighbors (KNN), Support Vector Machines (SVM), Extending Gradient Boosting (XGBoost) and Neural Networks (NNET). LDA, CVA, SVM, and NNET demonstrated greater accuracy and a F1-score >80%, even with a small and unbalanced sample size. The highest percentage of correctly assigned individuals was obtained in the Tagus estuary (mean 89%) and in the Albufeira lagoon (mean 93%), which were also the systems with more specimens measured (174 and 59 respectively), whereas the worst results were obtained in the Sado estuary (50%, 56 specimens). In the Albufeira coastal lagoon, the best classification methods reached 100% correct classifications. It further highlights the importance of establishing statistical standards, such as the ones developed in the current work, to evaluate different methods, as small changes in the procedure may cause substantial differences in the results and conclusions. The revision of previous works (presented as a table) showed often >90% of correct classification in both bivalves and gastropods, highlighting the potential of the techniques for other mollusks. Our results support the use of GM based on landmarks as a reliable tool for bivalve's traceability, since it is a quick, simple and inexpensive approach. Further research should extend these findings to other species and other shape analysis techniques.</p></div>","PeriodicalId":319,"journal":{"name":"Food Control","volume":null,"pages":null},"PeriodicalIF":5.6,"publicationDate":"2024-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0956713524003797/pdfft?md5=95187035c728ff5f8c676a41bb7c89a1&pid=1-s2.0-S0956713524003797-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141630193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The effect of UV-C light-activated gallic acid (AGA) alone or combined with ultrasound (US) on the inactivation of E. coli and S. Typhimurium inoculated in aqueous solution and lettuce was studied. The contact time (0–30 min) and AGA concentration (0.05–0.25 M) were evaluated against microorganisms inoculated in aqueous solutions. At selected conditions, the combined effect of AGA and US process was evaluated against microorganisms inoculated in lettuce leaves; samples were taken from washing peptone water and the surface of lettuce using a cotton swab. The microbial inactivation of AGA in lettuce was described using the Monod model, which adequately reproduces the inactivation kinetics (R > 0.96). In aqueous solutions, 10 min of contact time and 0.15 M and 0.25 M of AGA were required to reduce more than 5 log reduction of S. Typhimurium and E. coli, respectively. In lettuce, the E. coli showed higher resistance than S. Typhimurium to the AGA treatment, obtaining a maximum microbial reduction (4.33 ± 0.17 log reduction) using washing peptone water as a sampling method and AGA plus US process. Moreover, the AGA treatment combined with the US process presented a higher microbial inactivation compared to a common disinfectant (colloidal silver). Therefore, it is possible to indicate that AGA combined with the US may be an adequate alternative to reduce enteropathogens in lettuce.
研究了紫外线-C 光活化没食子酸(AGA)单独或与超声波(US)结合对接种在水溶液和莴苣中的大肠杆菌和伤寒杆菌的灭活效果。针对接种在水溶液中的微生物,对接触时间(0-30 分钟)和 AGA 浓度(0.05-0.25 M)进行了评估。在选定的条件下,评估了 AGA 和 US 工艺对接种在莴苣叶片中的微生物的综合影响;样品是用棉签从清洗蛋白胨水和莴苣表面采集的。使用莫诺模型描述了莴苣中 AGA 的微生物灭活过程,该模型充分再现了灭活动力学(R > 0.96)。在水溶液中,需要 10 分钟的接触时间以及 0.15 M 和 0.25 M 的 AGA 才能分别使伤寒杆菌和大肠杆菌减少 5 个对数值以上。在生菜中,大肠杆菌对 AGA 处理的耐受性高于鼠伤寒杆菌,使用洗涤蛋白胨水作为取样方法和 AGA 加 US 处理可获得最大的微生物减少量(4.33 ± 0.17 log 减少量)。此外,与普通消毒剂(胶体银)相比,AGA 处理加 US 处理的微生物灭活率更高。因此,可以说 AGA 与 US 工艺的结合是减少莴苣中肠道病原体的适当替代方法。
{"title":"UV-C light-activated gallic acid and ultrasound: A novel approach for reducing enteropathogens inoculated in lettuce (Lactuca sativa L.)","authors":"C.J. Alvarez-Rodríguez , N.X. Morales-Martínez , S.C. Beristain-Bauza , P. Hernández-Carranza , I.I. Ruiz-López , C.E. Ochoa-Velasco","doi":"10.1016/j.foodcont.2024.110673","DOIUrl":"https://doi.org/10.1016/j.foodcont.2024.110673","url":null,"abstract":"<div><p>The effect of UV-C light-activated gallic acid (AGA) alone or combined with ultrasound (US) on the inactivation of <em>E. coli</em> and <em>S</em>. Typhimurium inoculated in aqueous solution and lettuce was studied. The contact time (0–30 min) and AGA concentration (0.05–0.25 M) were evaluated against microorganisms inoculated in aqueous solutions. At selected conditions, the combined effect of AGA and US process was evaluated against microorganisms inoculated in lettuce leaves; samples were taken from washing peptone water and the surface of lettuce using a cotton swab. The microbial inactivation of AGA in lettuce was described using the Monod model, which adequately reproduces the inactivation kinetics (<em>R</em> > 0.96). In aqueous solutions, 10 min of contact time and 0.15 M and 0.25 M of AGA were required to reduce more than 5 log reduction of <em>S</em>. Typhimurium and <em>E. coli</em>, respectively. In lettuce, the <em>E. coli</em> showed higher resistance than <em>S</em>. Typhimurium to the AGA treatment, obtaining a maximum microbial reduction (4.33 ± 0.17 log reduction) using washing peptone water as a sampling method and AGA plus US process. Moreover, the AGA treatment combined with the US process presented a higher microbial inactivation compared to a common disinfectant (colloidal silver). Therefore, it is possible to indicate that AGA combined with the US may be an adequate alternative to reduce enteropathogens in lettuce.</p></div>","PeriodicalId":319,"journal":{"name":"Food Control","volume":null,"pages":null},"PeriodicalIF":5.6,"publicationDate":"2024-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141479869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-22DOI: 10.1016/j.foodcont.2024.110671
Meryeme El Maouardi , Kris De Braekeleer , Abdelaziz Bouklouze , Yvan Vander Heyden
Argan oil, a rare and luxury oil, is often adulterated with cheaper vegetable oils to make profits. Therefore, in this study, the potential of Mid-Infrared (MIR) and Near-Infrared (NIR) spectroscopy, along with chemometrics, for the rapid identification and quantification of argan oil adulteration, was investigated. First, the authentication of pure and adulterated samples was visually explored by Principal Component Analysis. MIR and NIR spectra allowed an excellent distinction between pure oil samples. Next, Partial Least Squares - Discriminant Analysis (PLS-DA) modelling was applied to discriminate between pure and adulterated argan oils. PLS-DA classification figures of merit, in terms of sensitivity, specificity, and accuracy, were very good for both NIR and MIR datasets. Finally, Partial Least Squares regression was used to model and predict the level of adulterant. The developed models showed a good performance, with RMSE values below 1.7% and coefficients of determination higher than 98% for both techniques.
{"title":"Comparison of Near-Infrared and Mid-Infrared spectroscopy for the identification and quantification of argan oil adulteration through PCA, PLS-DA and PLS","authors":"Meryeme El Maouardi , Kris De Braekeleer , Abdelaziz Bouklouze , Yvan Vander Heyden","doi":"10.1016/j.foodcont.2024.110671","DOIUrl":"https://doi.org/10.1016/j.foodcont.2024.110671","url":null,"abstract":"<div><p>Argan oil, a rare and luxury oil, is often adulterated with cheaper vegetable oils to make profits. Therefore, in this study, the potential of Mid-Infrared (MIR) and Near-Infrared (NIR) spectroscopy, along with chemometrics, for the rapid identification and quantification of argan oil adulteration, was investigated. First, the authentication of pure and adulterated samples was visually explored by Principal Component Analysis. MIR and NIR spectra allowed an excellent distinction between pure oil samples. Next, Partial Least Squares - Discriminant Analysis (PLS-DA) modelling was applied to discriminate between pure and adulterated argan oils. PLS-DA classification figures of merit, in terms of sensitivity, specificity, and accuracy, were very good for both NIR and MIR datasets. Finally, Partial Least Squares regression was used to model and predict the level of adulterant. The developed models showed a good performance, with RMSE values below 1.7% and coefficients of determination higher than 98% for both techniques.</p></div>","PeriodicalId":319,"journal":{"name":"Food Control","volume":null,"pages":null},"PeriodicalIF":5.6,"publicationDate":"2024-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141479871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-22DOI: 10.1016/j.foodcont.2024.110670
C. Sarai Reyes-Avila , Dominique Waldvogel , Nicolas Pradervand , Sylvain Aubry , Daniel Croll
The circulation of products based on genetically modified (GM) organisms is highly regulated by some governments with strict implementation rules for the breeding, planting, marketing, labelling, and trading of such products. To ensure compliance, accurate detection methods for GM events are necessary, along with assurance that GM material falls within relevant threshold levels. The increasing complexity and potential of undocumented GM are a growing challenge for genetic screening. Here, we developed and assessed a highly multiplexed amplicon sequencing assay for the detection of GM events based on a microfluidics platform and next-generation sequencing (NGS). To probe GM events comprehensively, we designed a total of 230 new amplicons to cover flanking, promoter, junction, and coding sequences of GM sequences. In addition, we designed and implemented parallel amplification of ribosomal and chloroplast markers to define crop species identity from potentially mixed samples. Using reference GM material of 11 crop species and multiple amplicons, we successfully detected the presence of 10 known modifications per GM event. We also find that reported flanking sequences of GM events may not be all useful for diagnostic. We assessed the assay's potential to detect GM events in mixed samples as well as in highly diluted DNA. Finally, we performed a prospective search of potentially undocumented GM events in plant material. Our microfluidics-based amplicon GM detection approach fills important gaps in detecting potentially undocumented and complex GM events by recovering a wide range of specific amplicon sequences for evaluation. Integrating highly parallel amplicon assays in GM screening efforts should be an effective complement to aid post-market monitoring and regulatory compliance efforts.
转基因产品的流通受到一些国家政府的严格监管,这些国家政府对转基因产品的育种、种植、销售、标签和交易制定了严格的实施细则。为确保合规,必须采用准确的转基因事件检测方法,同时确保转基因材料不超出相关阈值水平。无证转基因的复杂性和潜力不断增加,这对基因筛选是一个日益严峻的挑战。在此,我们开发并评估了一种基于微流控平台和下一代测序(NGS)的高复用扩增子测序检测方法,用于检测转基因事件。为了全面检测转基因事件,我们共设计了 230 个新的扩增子,以覆盖转基因序列的侧翼、启动子、连接序列和编码序列。此外,我们还设计并实施了核糖体和叶绿体标记的平行扩增,以便从潜在的混合样本中确定作物物种身份。利用 11 个作物物种的转基因参考材料和多个扩增子,我们成功检测出每个转基因事件中存在 10 个已知修饰。我们还发现,报告的转基因事件侧翼序列可能并不完全有助于诊断。我们评估了该检测方法在混合样本和高度稀释 DNA 中检测转基因事件的潜力。最后,我们对植物材料中可能未记录的转基因事件进行了前瞻性搜索。我们基于微流控技术的扩增片段转基因检测方法可回收各种特定的扩增片段序列进行评估,从而填补了在检测潜在未记录的复杂转基因事件方面的重要空白。在转基因筛查工作中整合高度平行的扩增子检测方法,应能有效辅助市场后监测和监管合规工作。
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Pub Date : 2024-06-22DOI: 10.1016/j.foodcont.2024.110672
Celine Verreydt , Emmanuel Cobbinah-Sam , Ana Mikler Celis , Martin Meckesheimer , Nikos Prountzos , Raphael Sacher , Elisabeth Tobler , Thijs Defraeye
To prevent postharvest losses, optimal fruit temperatures should be maintained. However, non-uniform hygrothermal conditions and hotspots can develop due to the dense stacking of the fruit inside refrigerated trailers. The question remains when and where they arise. In commercial shipments, only the air temperature at the back of the trailer is typically monitored. However, it is still unknown how this temperature relates to fruit temperatures within the cargo. This study experimentally measured air and fruit temperatures for citrus fruit transported from Greece to Switzerland within a commercial supply chain. 108 sensors were placed inside one shipment to map air and fruit temperature gradients and identify hotspots during precooling and transport. Besides, air and fruit temperatures were measured at the back of the trailer within 30 additional shipments to analyze the variations between different transports.
The results showed insufficient precooling (room precooling), as not all fruit reached the required setpoint temperature (4–6 °C) after 24 h of precooling. Although most of the field heat was removed during precooling, re-heating (>10 °C) of some fruit in the middle of the trailer was observed due to insufficient ventilation of the pallets. Finally, different shipments showed varying air temperatures (average interquartile range: 1.5 °C), where significant variations were observed between different shipments (up to 9 °C difference between the average temperatures) and where the measured air temperatures deviated from the setpoint. The results of this full-scale experiment indicate the importance of monitoring commercial supply chains to better understand transport processes within refrigerated trailers and prevent fruit losses.
{"title":"Identifying cooling heterogeneity during precooling and refrigerated trailer transport for the citrus supply chain by extensive temperature monitoring within full-scale experiments","authors":"Celine Verreydt , Emmanuel Cobbinah-Sam , Ana Mikler Celis , Martin Meckesheimer , Nikos Prountzos , Raphael Sacher , Elisabeth Tobler , Thijs Defraeye","doi":"10.1016/j.foodcont.2024.110672","DOIUrl":"https://doi.org/10.1016/j.foodcont.2024.110672","url":null,"abstract":"<div><p>To prevent postharvest losses, optimal fruit temperatures should be maintained. However, non-uniform hygrothermal conditions and hotspots can develop due to the dense stacking of the fruit inside refrigerated trailers. The question remains when and where they arise. In commercial shipments, only the air temperature at the back of the trailer is typically monitored. However, it is still unknown how this temperature relates to fruit temperatures within the cargo. This study experimentally measured air and fruit temperatures for citrus fruit transported from Greece to Switzerland within a commercial supply chain. 108 sensors were placed inside one shipment to map air and fruit temperature gradients and identify hotspots during precooling and transport. Besides, air and fruit temperatures were measured at the back of the trailer within 30 additional shipments to analyze the variations between different transports.</p><p>The results showed insufficient precooling (room precooling), as not all fruit reached the required setpoint temperature (4–6 °C) after 24 h of precooling. Although most of the field heat was removed during precooling, re-heating (>10 °C) of some fruit in the middle of the trailer was observed due to insufficient ventilation of the pallets. Finally, different shipments showed varying air temperatures (average interquartile range: 1.5 °C), where significant variations were observed between different shipments (up to 9 °C difference between the average temperatures) and where the measured air temperatures deviated from the setpoint. The results of this full-scale experiment indicate the importance of monitoring commercial supply chains to better understand transport processes within refrigerated trailers and prevent fruit losses.</p></div>","PeriodicalId":319,"journal":{"name":"Food Control","volume":null,"pages":null},"PeriodicalIF":5.6,"publicationDate":"2024-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S095671352400389X/pdfft?md5=f005e6db48a7ee082ca2d5fb029d672c&pid=1-s2.0-S095671352400389X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141541013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-21DOI: 10.1016/j.foodcont.2024.110659
N. Dadios , P.H. Imazaki , C. Millins , L.F. Thomas
Meatborne parasites pose significant public health concerns and lead to substantial economic losses. Decisions regarding the introduction of risk-based control strategies to manage these parasites depend on the availability of robust and comparable economic data. To understand the current economic evidence available on meatborne parasite control, a scoping study was conducted to provide recommendations on optimal investment. This study is a part of the COST Action CA18105 (Risk-based Meat Inspection and Integrated Meat Safety Assurance [RIBMINS]), which supports the development and implementation of risk-based meat inspection programs across the EU and partner countries. This study synthesized available evidence published between January 1, 1980, and April 1, 2022, on interventions for controlling four meatborne parasites (Taenia saginata, Taenia solium, T. gondii, and Trichinella spiralis) where economic analyses had been conducted in COST Action CA18105-affiliated countries. We considered interventions conducted at any node within meat value chains, including those undertaken by health systems targeting consumers. Twelve relevant articles related to eleven distinct studies were identified. The outcomes of economic analyses were recorded, and a critical appraisal of these studies was carried out. The economic data identified have been summarized narratively, and this scoping review has revealed a scarcity of economic data on which to base decisions regarding meatborne parasite control and a lack of standardization in the approaches used for economic analysis. We recommend consensus building among researchers working in the food safety assurance space to standardize the methods and reporting of economic analysis of interventions, similar to efforts that have been made in the health economics space.
{"title":"Economic evidence for the control of meatborne parasites in Europe: A scoping review","authors":"N. Dadios , P.H. Imazaki , C. Millins , L.F. Thomas","doi":"10.1016/j.foodcont.2024.110659","DOIUrl":"https://doi.org/10.1016/j.foodcont.2024.110659","url":null,"abstract":"<div><p>Meatborne parasites pose significant public health concerns and lead to substantial economic losses. Decisions regarding the introduction of risk-based control strategies to manage these parasites depend on the availability of robust and comparable economic data. To understand the current economic evidence available on meatborne parasite control, a scoping study was conducted to provide recommendations on optimal investment. This study is a part of the COST Action CA18105 (Risk-based Meat Inspection and Integrated Meat Safety Assurance [RIBMINS]), which supports the development and implementation of risk-based meat inspection programs across the EU and partner countries. This study synthesized available evidence published between January 1, 1980, and April 1, 2022, on interventions for controlling four meatborne parasites (<em>Taenia saginata</em>, <em>Taenia solium, T. gondii,</em> and <em>Trichinella spiralis</em>) where economic analyses had been conducted in COST Action CA18105-affiliated countries. We considered interventions conducted at any node within meat value chains, including those undertaken by health systems targeting consumers. Twelve relevant articles related to eleven distinct studies were identified. The outcomes of economic analyses were recorded, and a critical appraisal of these studies was carried out. The economic data identified have been summarized narratively, and this scoping review has revealed a scarcity of economic data on which to base decisions regarding meatborne parasite control and a lack of standardization in the approaches used for economic analysis. We recommend consensus building among researchers working in the food safety assurance space to standardize the methods and reporting of economic analysis of interventions, similar to efforts that have been made in the health economics space.</p></div>","PeriodicalId":319,"journal":{"name":"Food Control","volume":null,"pages":null},"PeriodicalIF":5.6,"publicationDate":"2024-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0956713524003761/pdfft?md5=5504873c1238f769b15518133e6e1990&pid=1-s2.0-S0956713524003761-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141484581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}