Pub Date : 1995-04-07DOI: 10.1109/SBEC.1995.514477
H. Ranu
A 3-D Foot Print Device has been used to measure the plantar foot pressures for normal subjects and diabetic patients with neurotrophic ulcers. The results confirm that patients with peripheral neuropathy develop over 40% higher forefoot pressures when compared with normal subjects.
{"title":"Gait analysis of diabetic foot","authors":"H. Ranu","doi":"10.1109/SBEC.1995.514477","DOIUrl":"https://doi.org/10.1109/SBEC.1995.514477","url":null,"abstract":"A 3-D Foot Print Device has been used to measure the plantar foot pressures for normal subjects and diabetic patients with neurotrophic ulcers. The results confirm that patients with peripheral neuropathy develop over 40% higher forefoot pressures when compared with normal subjects.","PeriodicalId":332563,"journal":{"name":"Proceedings of the 1995 Fourteenth Southern Biomedical Engineering Conference","volume":"9 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1995-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124388151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1995-04-07DOI: 10.1109/SBEC.1995.514488
G. Lewis, K. M. Shaw
The static electrochemical behavior of Ti-6Al-7Nb alloy specimens in three lactated Ringer's solution-based electrolytes was investigated using the d.c. polarization method. It was found that, relative to the case with Ringer's solution having pH = 6.25, decreasing the solution pH to 1 increases the corrosion rate of the alloy but adding bovine serum to the solution only marginally affects the rate. A critical appraisal of the polarization method is presented, and comments on the clinical usefulness of the results are included.
{"title":"Orthopaedic alloy electrochemical behavior: the case of Ti-6Al-7Nb","authors":"G. Lewis, K. M. Shaw","doi":"10.1109/SBEC.1995.514488","DOIUrl":"https://doi.org/10.1109/SBEC.1995.514488","url":null,"abstract":"The static electrochemical behavior of Ti-6Al-7Nb alloy specimens in three lactated Ringer's solution-based electrolytes was investigated using the d.c. polarization method. It was found that, relative to the case with Ringer's solution having pH = 6.25, decreasing the solution pH to 1 increases the corrosion rate of the alloy but adding bovine serum to the solution only marginally affects the rate. A critical appraisal of the polarization method is presented, and comments on the clinical usefulness of the results are included.","PeriodicalId":332563,"journal":{"name":"Proceedings of the 1995 Fourteenth Southern Biomedical Engineering Conference","volume":"21 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1995-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126668175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1995-04-07DOI: 10.1109/SBEC.1995.514505
H. Benghuzzi, M. Tucci
The objective of this study was to investigate the effect of various biomedical polymers on the adhesion rate of HeLa cells as a model. The HeLa cells used in this study seeded by following our standard laboratory procedure. A total of 1/spl times/10/sup 5/ cells were plated in each of the pretreated wells with various concentrations of (0.01, 0.1, and 1% wt/vol) polyvaline (P-Val), polyalanine (p-Ala), polytryptophan (P-Trp) and buffered control. At the end of 1,4, and 24 hours the assay was developed by following standard lab procedure. The final determination was achieved by means of ELIZA. The data obtained from this study suggest that (i) the rate of HeLa spreading was strongly influenced by both incubation time and the polymer concentration, (ii) it is apparent that polymer treated wells within the first hour facilitated attachment of HeLa cells to their surfaces for all concentrations tested with the exception of 1% alanine. This observation confirms our previous results using different cell lines and provides a valuable information for experiments that need to be performed in a short period of time with the maximum amount of cells attached, (iii) the surface attachment of HeLa to P-Ala, P-Val, and P-Trp were demonstrated to vary depending on their chemical structure and level of microporosity. Thus, overall observation led us to conclude that the surface reactivity of P-Ala, P-Val, and P-Trp be always taken into account in discussing their biocompatibility using transformed cells such as HeLa cells as a model.
{"title":"The effect of P-Trp, P-Ala and P-Val on the proliferation rate of HeLa cells in culture","authors":"H. Benghuzzi, M. Tucci","doi":"10.1109/SBEC.1995.514505","DOIUrl":"https://doi.org/10.1109/SBEC.1995.514505","url":null,"abstract":"The objective of this study was to investigate the effect of various biomedical polymers on the adhesion rate of HeLa cells as a model. The HeLa cells used in this study seeded by following our standard laboratory procedure. A total of 1/spl times/10/sup 5/ cells were plated in each of the pretreated wells with various concentrations of (0.01, 0.1, and 1% wt/vol) polyvaline (P-Val), polyalanine (p-Ala), polytryptophan (P-Trp) and buffered control. At the end of 1,4, and 24 hours the assay was developed by following standard lab procedure. The final determination was achieved by means of ELIZA. The data obtained from this study suggest that (i) the rate of HeLa spreading was strongly influenced by both incubation time and the polymer concentration, (ii) it is apparent that polymer treated wells within the first hour facilitated attachment of HeLa cells to their surfaces for all concentrations tested with the exception of 1% alanine. This observation confirms our previous results using different cell lines and provides a valuable information for experiments that need to be performed in a short period of time with the maximum amount of cells attached, (iii) the surface attachment of HeLa to P-Ala, P-Val, and P-Trp were demonstrated to vary depending on their chemical structure and level of microporosity. Thus, overall observation led us to conclude that the surface reactivity of P-Ala, P-Val, and P-Trp be always taken into account in discussing their biocompatibility using transformed cells such as HeLa cells as a model.","PeriodicalId":332563,"journal":{"name":"Proceedings of the 1995 Fourteenth Southern Biomedical Engineering Conference","volume":"44 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1995-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128449415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1995-04-07DOI: 10.1109/SBEC.1995.514503
M. Langford, J. Schulman, D. P. Mukherjee, J. Ganley
Hyaluronic acid (HA) and perfluorocarbon liquid (PFCL) are used during and after intraocular surgery to maintain normal ocular structure. We present the results of our studies on the effects of vertebrate HA (Healon), N,O carboxymethyl Chitosan (CMC), and PFCL (perfluorophenanthrene, Vitreon) on conjunctival epithelial cells and human peripheral blood leukocytes (PBL). No cytotoxic effects were observed with HA or CMC on conjunctival epithelial cells. PFCL flattened cultured conjunctival cells and was cytocidal. CMC was associated with the formation of monocyte/macrophage syncytia in mitogen stimulated PBL cultures. CMC and PFCL inhibited PBL proliferation and interferon-/spl gamma/ (IFN-/spl gamma/) production by phytohemagglutinin (PHA), concanavalin A (ConA) and Staphylococcal enterotoxin B (SEB) stimulated PBL. Our results indicate that HA and CMC are not toxic to conjunctival epithelial cells but suggest that CMC may affect monocyte/macrophage functions associated with T cell proliferation and IFN-/spl gamma/ production. PFCL is cytocidal to conjunctival epithelial cells in stationary cultures and suppresses PBL proliferation and IFN-/spl gamma/ production.
{"title":"Effects of Healon, N,O carboxymethyl Chitosan and Vitreon on conjunctival cells and human peripheral blood lymphocytes","authors":"M. Langford, J. Schulman, D. P. Mukherjee, J. Ganley","doi":"10.1109/SBEC.1995.514503","DOIUrl":"https://doi.org/10.1109/SBEC.1995.514503","url":null,"abstract":"Hyaluronic acid (HA) and perfluorocarbon liquid (PFCL) are used during and after intraocular surgery to maintain normal ocular structure. We present the results of our studies on the effects of vertebrate HA (Healon), N,O carboxymethyl Chitosan (CMC), and PFCL (perfluorophenanthrene, Vitreon) on conjunctival epithelial cells and human peripheral blood leukocytes (PBL). No cytotoxic effects were observed with HA or CMC on conjunctival epithelial cells. PFCL flattened cultured conjunctival cells and was cytocidal. CMC was associated with the formation of monocyte/macrophage syncytia in mitogen stimulated PBL cultures. CMC and PFCL inhibited PBL proliferation and interferon-/spl gamma/ (IFN-/spl gamma/) production by phytohemagglutinin (PHA), concanavalin A (ConA) and Staphylococcal enterotoxin B (SEB) stimulated PBL. Our results indicate that HA and CMC are not toxic to conjunctival epithelial cells but suggest that CMC may affect monocyte/macrophage functions associated with T cell proliferation and IFN-/spl gamma/ production. PFCL is cytocidal to conjunctival epithelial cells in stationary cultures and suppresses PBL proliferation and IFN-/spl gamma/ production.","PeriodicalId":332563,"journal":{"name":"Proceedings of the 1995 Fourteenth Southern Biomedical Engineering Conference","volume":"25 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1995-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134351970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1995-04-07DOI: 10.1109/SBEC.1995.514423
N. Mapara, N. F. MacLean, D. Steinman, D. Holdsworth, M. R. Roach
The formation of separation zones in arteries may have important implications in the development of atherosclerotic lesions. It is known that arteries flare proximally to bifurcations where atherosclerotic lesions develop. Flow through an axisymmetric flared cylinder, which simulated the human infrarenal aorta, was studied using FIDAP, a finite element analysis program. Blood was assumed to have Newtonian properties while the flow was assumed to be steady and laminar. The wall of the artery was considered to be rigid. For this study, the angle of flare was varied from 1/spl deg/ to 5/spl deg/ with an inlet to outlet diameter ratio of 0.33. Reynolds numbers for the simulation ranged from 100 to 1000. A critical Reynolds number existed below which no separation zone was formed. At Reynolds numbers greater than this critical value, recirculation zones formed downstream of the flare. A linear relationship was observed between the length of the recirculation zone and the Reynolds number. There was an inverse, non-linear relationship between the critical Reynolds number and the flaring angle. The length of the recirculation zone was more sensitive to changes in Reynolds numbers at higher flaring angles.
{"title":"Numerical simulations of flow in flared sections of the human infrarenal aorta","authors":"N. Mapara, N. F. MacLean, D. Steinman, D. Holdsworth, M. R. Roach","doi":"10.1109/SBEC.1995.514423","DOIUrl":"https://doi.org/10.1109/SBEC.1995.514423","url":null,"abstract":"The formation of separation zones in arteries may have important implications in the development of atherosclerotic lesions. It is known that arteries flare proximally to bifurcations where atherosclerotic lesions develop. Flow through an axisymmetric flared cylinder, which simulated the human infrarenal aorta, was studied using FIDAP, a finite element analysis program. Blood was assumed to have Newtonian properties while the flow was assumed to be steady and laminar. The wall of the artery was considered to be rigid. For this study, the angle of flare was varied from 1/spl deg/ to 5/spl deg/ with an inlet to outlet diameter ratio of 0.33. Reynolds numbers for the simulation ranged from 100 to 1000. A critical Reynolds number existed below which no separation zone was formed. At Reynolds numbers greater than this critical value, recirculation zones formed downstream of the flare. A linear relationship was observed between the length of the recirculation zone and the Reynolds number. There was an inverse, non-linear relationship between the critical Reynolds number and the flaring angle. The length of the recirculation zone was more sensitive to changes in Reynolds numbers at higher flaring angles.","PeriodicalId":332563,"journal":{"name":"Proceedings of the 1995 Fourteenth Southern Biomedical Engineering Conference","volume":"139 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1995-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134399292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1995-04-07DOI: 10.1109/SBEC.1995.514492
T. Maruyama, Subrata Saha, D. Mongiano, K. Mudge
The mechanical strength of human metacarpal fractures fixed with sintered self-reinforced polyglycolide (SR-PGA) rods was evaluated. The surgical constructs were immersed in buffered saline solution and maintained at body temperature (37/spl deg/C). The initial bending stiffness of the specimens fixed with SR-PGA rods was 61% of that of Kirschner wire fixation. The bending stiffness of the specimens fixed with absorbable rods decreased with time. Most of the specimens lost their mechanical stability at four weeks.
{"title":"A biomechanical evaluation of metacarpal fracture fixation with absorbable polyglycolide rods","authors":"T. Maruyama, Subrata Saha, D. Mongiano, K. Mudge","doi":"10.1109/SBEC.1995.514492","DOIUrl":"https://doi.org/10.1109/SBEC.1995.514492","url":null,"abstract":"The mechanical strength of human metacarpal fractures fixed with sintered self-reinforced polyglycolide (SR-PGA) rods was evaluated. The surgical constructs were immersed in buffered saline solution and maintained at body temperature (37/spl deg/C). The initial bending stiffness of the specimens fixed with SR-PGA rods was 61% of that of Kirschner wire fixation. The bending stiffness of the specimens fixed with absorbable rods decreased with time. Most of the specimens lost their mechanical stability at four weeks.","PeriodicalId":332563,"journal":{"name":"Proceedings of the 1995 Fourteenth Southern Biomedical Engineering Conference","volume":"107 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1995-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134483797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1995-04-07DOI: 10.1109/SBEC.1995.514441
K. Coward, B. Smith, R. N. Kruse, D. P. Mukherjee
An in vitro study was done using a bovine rib model to evaluate three different plates designed for mandibular fracture fixation. The types of plates tested were the Synthes II, Synthes X, and the Leibinger 3D. The fixated osteotomized rib was loaded up to 150 N at a distance 10 cm from the holding fixture. The superior gap and displacement were measured at 25 N increments for the ribs fixated by each of the plates. The Synthes II plate gave the best fixation having the smallest gap and displacement values.
用牛肋骨模型进行了体外研究，评估了用于下颌骨折固定的三种不同的钢板。测试的板类型是Synthes II, Synthes X和Leibinger 3D。将固定的去骨肋骨在距固定装置10厘米处加载至150牛。每个钢板固定的肋骨以25 N的增量测量上部间隙和位移。Synthes II钢板具有最小的间隙和位移值，具有最佳的固定效果。
{"title":"A biomechanical study of mandibular fracture fixation plates in a bovine rib model","authors":"K. Coward, B. Smith, R. N. Kruse, D. P. Mukherjee","doi":"10.1109/SBEC.1995.514441","DOIUrl":"https://doi.org/10.1109/SBEC.1995.514441","url":null,"abstract":"An in vitro study was done using a bovine rib model to evaluate three different plates designed for mandibular fracture fixation. The types of plates tested were the Synthes II, Synthes X, and the Leibinger 3D. The fixated osteotomized rib was loaded up to 150 N at a distance 10 cm from the holding fixture. The superior gap and displacement were measured at 25 N increments for the ribs fixated by each of the plates. The Synthes II plate gave the best fixation having the smallest gap and displacement values.","PeriodicalId":332563,"journal":{"name":"Proceedings of the 1995 Fourteenth Southern Biomedical Engineering Conference","volume":"42 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1995-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121374031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1995-04-07DOI: 10.1109/SBEC.1995.514450
K. Deisseroth, H. Hogan
This study provides a qualitative as well as quantitative characterization of the geometry and architecture of the Haversian systems at a microscopic level. A three dimensional computer model was created with serial sections of equine bone. From the reconstruction, quantitative calculations were made, including porosity, surface areas, cross sectional areas, and cross sectional shapes. Also the porosity due to Volkmann canals was estimated. Other more qualitative calculations included the nature of branching, termination and general osteon movement. Any change in the specimen size or shape during the entire processing was carefully documented and corrected for where possible. One of the goals of the research was to assess and evaluate the procedures developed and identify was to further improve accuracy. With a computerized three dimensional image of the bone microstructure, detailed calculations can be made and insight gained more accurately than with two dimensional models or past three dimensional models.
{"title":"Three dimensional modeling and analysis of Haversian systems in compact bone tissue","authors":"K. Deisseroth, H. Hogan","doi":"10.1109/SBEC.1995.514450","DOIUrl":"https://doi.org/10.1109/SBEC.1995.514450","url":null,"abstract":"This study provides a qualitative as well as quantitative characterization of the geometry and architecture of the Haversian systems at a microscopic level. A three dimensional computer model was created with serial sections of equine bone. From the reconstruction, quantitative calculations were made, including porosity, surface areas, cross sectional areas, and cross sectional shapes. Also the porosity due to Volkmann canals was estimated. Other more qualitative calculations included the nature of branching, termination and general osteon movement. Any change in the specimen size or shape during the entire processing was carefully documented and corrected for where possible. One of the goals of the research was to assess and evaluate the procedures developed and identify was to further improve accuracy. With a computerized three dimensional image of the bone microstructure, detailed calculations can be made and insight gained more accurately than with two dimensional models or past three dimensional models.","PeriodicalId":332563,"journal":{"name":"Proceedings of the 1995 Fourteenth Southern Biomedical Engineering Conference","volume":"90 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1995-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126449641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1995-04-07DOI: 10.1109/SBEC.1995.514456
L. Kooragayala, J. Schulman, A. Pramanik, P. Tseng
Cryotherapy is the accepted method to treat retinopathy of prematurity (ROP). The Cryotherapy for Retinopathy of Prematurity Cooperative Group conducted a prospective randomized study which established the beneficial effectiveness of cryopexy as a treatment modality for threshold ROP. Despite demonstrating the overall beneficial effects of cryotherapy, a small subgroup of infants in the study with zone-I threshold ROP showed either no beneficial effects or at most a minimal reduction in unfavorable events in eyes treated with cryotherapy. The failure rate was reduced from 91.77% to 75% when comparing control to treated eyes although only twelve eyes were treated. Based on these poor treatment results and significant advances in laser technology a renewed interest has developed in the use of laser photocoagulation to treat eyes with zone I threshold ROP. Delivery of thermal laser spots to the retinal periphery has been simplified following development of an indirect laser delivery system. Additional interest has been stimulated by the recent commercial availability of the compact solid state diode lasers. This laser, unlike older gas ion lasers has a lower cost, does not require any ancillary cooling, operates on standard voltage requirements and in some instances is capable of being driven by batteries. A diode laser as a result can easily transported to, and operated in, the neonatal intensive care unit. The authors present the results of a prospective study evaluating diode laser photocoagulation in the management of 23 consecutive premature infant eyes with zone I threshold ROP. The mean follow-up was 53.6 weeks. A favorable outcome was achieved in 22 of the 23 eyes. Various features of the diode laser which appeared to significantly contribute to these improved treatment results are discussed.
{"title":"Advances in laser technology assist in treatment for retinopathy of prematurity","authors":"L. Kooragayala, J. Schulman, A. Pramanik, P. Tseng","doi":"10.1109/SBEC.1995.514456","DOIUrl":"https://doi.org/10.1109/SBEC.1995.514456","url":null,"abstract":"Cryotherapy is the accepted method to treat retinopathy of prematurity (ROP). The Cryotherapy for Retinopathy of Prematurity Cooperative Group conducted a prospective randomized study which established the beneficial effectiveness of cryopexy as a treatment modality for threshold ROP. Despite demonstrating the overall beneficial effects of cryotherapy, a small subgroup of infants in the study with zone-I threshold ROP showed either no beneficial effects or at most a minimal reduction in unfavorable events in eyes treated with cryotherapy. The failure rate was reduced from 91.77% to 75% when comparing control to treated eyes although only twelve eyes were treated. Based on these poor treatment results and significant advances in laser technology a renewed interest has developed in the use of laser photocoagulation to treat eyes with zone I threshold ROP. Delivery of thermal laser spots to the retinal periphery has been simplified following development of an indirect laser delivery system. Additional interest has been stimulated by the recent commercial availability of the compact solid state diode lasers. This laser, unlike older gas ion lasers has a lower cost, does not require any ancillary cooling, operates on standard voltage requirements and in some instances is capable of being driven by batteries. A diode laser as a result can easily transported to, and operated in, the neonatal intensive care unit. The authors present the results of a prospective study evaluating diode laser photocoagulation in the management of 23 consecutive premature infant eyes with zone I threshold ROP. The mean follow-up was 53.6 weeks. A favorable outcome was achieved in 22 of the 23 eyes. Various features of the diode laser which appeared to significantly contribute to these improved treatment results are discussed.","PeriodicalId":332563,"journal":{"name":"Proceedings of the 1995 Fourteenth Southern Biomedical Engineering Conference","volume":"10880 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1995-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133770648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1995-04-07DOI: 10.1109/SBEC.1995.514454
S. Salkeld, S. Cook, D. Rueger
Recombinant human osteogenic protein-1 (OP-1) was combined with a series of nonproteinaceous polymeric carriers in varying dosages. The implants were placed in critical sized bilateral ulnar segmental defects of thirty rabbits and twelve dogs. Animals were followed radiographically every two weeks to evaluate the rate and amount of bone formation. Rabbits were sacrificed at eight weeks and dogs were sacrificed at twelve weeks postimplantation. Post-sacrifice mechanical testing and histologic analysis was performed on the retrieved specimens. Sequential radiographs demonstrated new bone formation in all OP-1/polymer sites. The healed polymeric/OP-1 defects demonstrated torsional strength approaching intact controls and OP-1/collagen. This investigation demonstrates that polymeric carriers with the appropriate dosage and degradation characteristics may be used to deliver OP-1 to heal large segmental bone defects.
{"title":"Synthetic polymers as carriers for osteogenic proteins","authors":"S. Salkeld, S. Cook, D. Rueger","doi":"10.1109/SBEC.1995.514454","DOIUrl":"https://doi.org/10.1109/SBEC.1995.514454","url":null,"abstract":"Recombinant human osteogenic protein-1 (OP-1) was combined with a series of nonproteinaceous polymeric carriers in varying dosages. The implants were placed in critical sized bilateral ulnar segmental defects of thirty rabbits and twelve dogs. Animals were followed radiographically every two weeks to evaluate the rate and amount of bone formation. Rabbits were sacrificed at eight weeks and dogs were sacrificed at twelve weeks postimplantation. Post-sacrifice mechanical testing and histologic analysis was performed on the retrieved specimens. Sequential radiographs demonstrated new bone formation in all OP-1/polymer sites. The healed polymeric/OP-1 defects demonstrated torsional strength approaching intact controls and OP-1/collagen. This investigation demonstrates that polymeric carriers with the appropriate dosage and degradation characteristics may be used to deliver OP-1 to heal large segmental bone defects.","PeriodicalId":332563,"journal":{"name":"Proceedings of the 1995 Fourteenth Southern Biomedical Engineering Conference","volume":"118 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1995-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123246967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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