Pub Date : 2009-12-11DOI: 10.1109/MHS.2009.5351934
M. Mukai, A. Honda
Ebp1 is a member of the PA2G4 family protein and initially isolated as an ErB3 (an epidermal receptor tyrosine kinase) binding protein. Ebp1 inhibits cell growth and repress transcription of E2F-regulated cell cycle genes. Previously, we reported that Ebp1 protein interacted with RNA polymerase subunit PB1 of the influenza virus and disturbed in vitro RNA synthesis by influenza RNA polymerase. Recently we found that after influenza virus infection to the cell, Ebp1 expression is induced at 4 hours after viral infection. By using reverse genetics method, Ebp1 inhibits influenza virus replication. Ebp1 expression mechanism is very interesting because the expression of Ebp1 is earlier than that of virus proteins. In addition, in uninfection cell, Ebp1 is expressed from G1 to S phase in cell cycle-dependent manner. Therefore we study to identify the transcription factor of Ebp1 and understand the Ebp1 expression mechanism by influenza viral infection. Ebp1 promoter region was cloned into pTurboGFP. A pTurboGFP-Ebp1 promoter plasmid was mixed with nucleus extract form infection / uninfection cell. The binding proteins were eluted and analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). We identified several candidate proteins.
{"title":"Analysis of promoter binding proteins of Ebp1 that is inhibitor protein of influenza virus RNA polymerase","authors":"M. Mukai, A. Honda","doi":"10.1109/MHS.2009.5351934","DOIUrl":"https://doi.org/10.1109/MHS.2009.5351934","url":null,"abstract":"Ebp1 is a member of the PA2G4 family protein and initially isolated as an ErB3 (an epidermal receptor tyrosine kinase) binding protein. Ebp1 inhibits cell growth and repress transcription of E2F-regulated cell cycle genes. Previously, we reported that Ebp1 protein interacted with RNA polymerase subunit PB1 of the influenza virus and disturbed in vitro RNA synthesis by influenza RNA polymerase. Recently we found that after influenza virus infection to the cell, Ebp1 expression is induced at 4 hours after viral infection. By using reverse genetics method, Ebp1 inhibits influenza virus replication. Ebp1 expression mechanism is very interesting because the expression of Ebp1 is earlier than that of virus proteins. In addition, in uninfection cell, Ebp1 is expressed from G1 to S phase in cell cycle-dependent manner. Therefore we study to identify the transcription factor of Ebp1 and understand the Ebp1 expression mechanism by influenza viral infection. Ebp1 promoter region was cloned into pTurboGFP. A pTurboGFP-Ebp1 promoter plasmid was mixed with nucleus extract form infection / uninfection cell. The binding proteins were eluted and analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). We identified several candidate proteins.","PeriodicalId":344667,"journal":{"name":"2009 International Symposium on Micro-NanoMechatronics and Human Science","volume":"39 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2009-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114808513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-12-11DOI: 10.1109/MHS.2009.5351734
Kaoru Uesugi, Y. Akiyama, M. Yamato, T. Okano, T. Hoshino, K. Morishima
We have demonstrated hybrid micro mechanical systems (HMMS) using muscle cells. When constructing cells to HMMS, there are some forces between adhesive interface and cells, dur to contraction of muscle cells must be considered to design. When these forces are larger than adhesion force of cells, cells detach from substrates and system is not functional. Measuring the adhesion force of cells could prevent from the breakage of the system. In this paper, we developed cell adhesion force measurement system “90 degree detachment examination” which defined by “International Organization for Standardization (ISO)”. To attempt 90 degree detachment examination, we noticed that to use cell sheet which cultured confluent on two-dimensional surface. And to use cell sheet, we develop four types of cell sheet handling tools. As a result, we measured maximum adhesion strength of cells about 10 N/mm.
{"title":"Development of cell-sheet handling tool for measurement of cell sheet adhesion force","authors":"Kaoru Uesugi, Y. Akiyama, M. Yamato, T. Okano, T. Hoshino, K. Morishima","doi":"10.1109/MHS.2009.5351734","DOIUrl":"https://doi.org/10.1109/MHS.2009.5351734","url":null,"abstract":"We have demonstrated hybrid micro mechanical systems (HMMS) using muscle cells. When constructing cells to HMMS, there are some forces between adhesive interface and cells, dur to contraction of muscle cells must be considered to design. When these forces are larger than adhesion force of cells, cells detach from substrates and system is not functional. Measuring the adhesion force of cells could prevent from the breakage of the system. In this paper, we developed cell adhesion force measurement system “90 degree detachment examination” which defined by “International Organization for Standardization (ISO)”. To attempt 90 degree detachment examination, we noticed that to use cell sheet which cultured confluent on two-dimensional surface. And to use cell sheet, we develop four types of cell sheet handling tools. As a result, we measured maximum adhesion strength of cells about 10 N/mm.","PeriodicalId":344667,"journal":{"name":"2009 International Symposium on Micro-NanoMechatronics and Human Science","volume":"31 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2009-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125298145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-12-11DOI: 10.1109/MHS.2009.5351873
H. Maruyama, F. Arai
Functional gel-nanotool impregnated with indicators for intracellular measurement in a microfluidic chip was developed. We employed membrane fusion of liposome for invasive injection of the gel-nanotool into a specific cell. The gel-nanotool is made by photo polymerization of the aggregated hydrophilic photo-crosslinkable resin. And it is modified by fluorescent dye for environment measurement. For injection to a cell, the gel-nanotool was included in fusogenic liposome. The gel-nanotool coated by fusogenic liposome was prepared by Bangham method. The gel-nanotool was manipulated by optical tweezers and contacted to the target cell. After contact of the gel-nanotool, the gel-nanotool was injected to the cell autonomously by membrane fusion of the fusogenic liposome. Injected gel-nanotool was manipulated by optical tweezers inside the cell. Detection of intracellular temperature was performed by observing the fluorescence from the gel-nanotool. We demonstrated preparation of the gel-nanotool, injection of the gel-nanotool to the cell by membrane fusion, and measurement of intracellular temperature in the chip.
{"title":"Fabrication of functional gel-nanotool for intracellular measurement","authors":"H. Maruyama, F. Arai","doi":"10.1109/MHS.2009.5351873","DOIUrl":"https://doi.org/10.1109/MHS.2009.5351873","url":null,"abstract":"Functional gel-nanotool impregnated with indicators for intracellular measurement in a microfluidic chip was developed. We employed membrane fusion of liposome for invasive injection of the gel-nanotool into a specific cell. The gel-nanotool is made by photo polymerization of the aggregated hydrophilic photo-crosslinkable resin. And it is modified by fluorescent dye for environment measurement. For injection to a cell, the gel-nanotool was included in fusogenic liposome. The gel-nanotool coated by fusogenic liposome was prepared by Bangham method. The gel-nanotool was manipulated by optical tweezers and contacted to the target cell. After contact of the gel-nanotool, the gel-nanotool was injected to the cell autonomously by membrane fusion of the fusogenic liposome. Injected gel-nanotool was manipulated by optical tweezers inside the cell. Detection of intracellular temperature was performed by observing the fluorescence from the gel-nanotool. We demonstrated preparation of the gel-nanotool, injection of the gel-nanotool to the cell by membrane fusion, and measurement of intracellular temperature in the chip.","PeriodicalId":344667,"journal":{"name":"2009 International Symposium on Micro-NanoMechatronics and Human Science","volume":"35 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2009-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126751414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-12-11DOI: 10.1109/MHS.2009.5351964
A. Ichikawa, T. Tanikawa, K. Ohba
The purpose of our research work is to develop a multifunctional piezoelectric actuation mechanism for micro-manipulator using 3 degree of freedom actuation plate and expansion plate which its expansion rate and its character frequency can be adjusted by the number of the laminated hinge mechanism. It is important to downsizing of actuation mechanism along with the miniaturization of electronic circuit devices, moreover the making to high accuracy and integration of the actuator are also needed. The expansion mechanism is made by thin metal plates which have hinge mechanism. The expansion rate can be controlled by the number of the expansion plates. The expansion mechanism is small and useful to make micro-manipulator which can be set up on a microscope stage. This report shows a principle of the hinge mechanism, systems of micro-manipulator and result of the manipulation experiment.
{"title":"Micro-manipulator using laminate hinge mechanism","authors":"A. Ichikawa, T. Tanikawa, K. Ohba","doi":"10.1109/MHS.2009.5351964","DOIUrl":"https://doi.org/10.1109/MHS.2009.5351964","url":null,"abstract":"The purpose of our research work is to develop a multifunctional piezoelectric actuation mechanism for micro-manipulator using 3 degree of freedom actuation plate and expansion plate which its expansion rate and its character frequency can be adjusted by the number of the laminated hinge mechanism. It is important to downsizing of actuation mechanism along with the miniaturization of electronic circuit devices, moreover the making to high accuracy and integration of the actuator are also needed. The expansion mechanism is made by thin metal plates which have hinge mechanism. The expansion rate can be controlled by the number of the expansion plates. The expansion mechanism is small and useful to make micro-manipulator which can be set up on a microscope stage. This report shows a principle of the hinge mechanism, systems of micro-manipulator and result of the manipulation experiment.","PeriodicalId":344667,"journal":{"name":"2009 International Symposium on Micro-NanoMechatronics and Human Science","volume":"23 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2009-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124845219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-12-11DOI: 10.1109/MHS.2009.5351738
S. Minakata, J. Usukura
Actin filaments play central roles in shape determination, cytokinesis, and cell motility. Recently, we found a new type of actin filament that is seemed to contact tightly membrane surface and be buried into membrane under electron microscope. However, the function of this actin filament is not obvious. Then, we have reproduced of such actin filaments using a lipid layer instead of the cell membrane cytoskeleton. We confirmed that the several actin filaments extended on the lipid layer while contacting firmly. The appearance of actin filaments on the lipid layer is quite similar to the new type of actin filaments buried in the cell membrane.
{"title":"The reconstitution of the membrane cytoskeleton using a lipid layer","authors":"S. Minakata, J. Usukura","doi":"10.1109/MHS.2009.5351738","DOIUrl":"https://doi.org/10.1109/MHS.2009.5351738","url":null,"abstract":"Actin filaments play central roles in shape determination, cytokinesis, and cell motility. Recently, we found a new type of actin filament that is seemed to contact tightly membrane surface and be buried into membrane under electron microscope. However, the function of this actin filament is not obvious. Then, we have reproduced of such actin filaments using a lipid layer instead of the cell membrane cytoskeleton. We confirmed that the several actin filaments extended on the lipid layer while contacting firmly. The appearance of actin filaments on the lipid layer is quite similar to the new type of actin filaments buried in the cell membrane.","PeriodicalId":344667,"journal":{"name":"2009 International Symposium on Micro-NanoMechatronics and Human Science","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2009-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131392909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-12-11DOI: 10.1109/MHS.2009.5351761
W. Jatmiko, W. Pambuko, P. Mursanto, A. Muis, B. Kusumoputro, K. Sekiyama, T. Fukuda
A new algorithm based on Modified Particle Swarm Optimization (MPSO) which follows a local gradient of the chemical concentration within a plume and follow direction of the wind velocity is investigated. Moreover, the niche or parallel search characteristic is adopted on MPSO to solve the multi-peak and multi-source problem. When using parallel MPSO, subgroup of robot is introduced then each subgroup can locate the odor source. Unfortunately, there is a possibility that more that one subgroup locates one odor sources. This is inefficient because other subgroups locate other source, then we proposed a ranged subgroup method for coping for that problem, then the searching performance will increase. Then ODE (Open Dynamics Engine) library is used for physical modeling of the robot like friction, balancing moment and others. Finally the statistical analysis shows that the new approach is technically sounds.
{"title":"Localizing multiple odor sources in dynamic environment using ranged subgroup PSO with flow of wind based on open dynamic engine library","authors":"W. Jatmiko, W. Pambuko, P. Mursanto, A. Muis, B. Kusumoputro, K. Sekiyama, T. Fukuda","doi":"10.1109/MHS.2009.5351761","DOIUrl":"https://doi.org/10.1109/MHS.2009.5351761","url":null,"abstract":"A new algorithm based on Modified Particle Swarm Optimization (MPSO) which follows a local gradient of the chemical concentration within a plume and follow direction of the wind velocity is investigated. Moreover, the niche or parallel search characteristic is adopted on MPSO to solve the multi-peak and multi-source problem. When using parallel MPSO, subgroup of robot is introduced then each subgroup can locate the odor source. Unfortunately, there is a possibility that more that one subgroup locates one odor sources. This is inefficient because other subgroups locate other source, then we proposed a ranged subgroup method for coping for that problem, then the searching performance will increase. Then ODE (Open Dynamics Engine) library is used for physical modeling of the robot like friction, balancing moment and others. Finally the statistical analysis shows that the new approach is technically sounds.","PeriodicalId":344667,"journal":{"name":"2009 International Symposium on Micro-NanoMechatronics and Human Science","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2009-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132457777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-12-11DOI: 10.1109/MHS.2009.5351819
J. Teramoto, Kayoko Yamada, N. Kobayashi, A. Kori, S. Yoshimura, K. Takeyasu, A. Ishihama
A systematic search has been performed for DNA-binding sequences recognized by YgiP, an uncharacterized transcription factor of Escherichia coli, using the newly developed Genomic SELEX. By SELEX-clos procedure, a total of 333 sequences have been isolated from a total of 126 loci of the E. coli genome while more than 703 YgiP-binding loci have been identified after the genome-wide profiling by SELEX-chip procedure. Gel mobility shift assay indicated the presence of multiple YgiP-binding sites along each SELEX DNA fragment while DNase-I foot-printing assay indicated protection of short-specific sequences by YgiP, together suggesting YgiP as a nucleoid protein with broad specificity of DNA binding. Atomic force microscope (AFM) observation indicated that at low concentrations, YgiP binds to various sites in non-specific manner, but at high concentrations, YgiP covers the entire DNA surface. The intracellular level of YgiP was found to be very low in steady-state of cell growth under aerobic conditions, but increased more than 100-fold to the level as high as those of HU and IHF under hypoxic or anaerobic culture conditions. An E. coli mutant lacking ygiP showed abnormal growth under anaerobic conditions. Taken together we conclude that YgiP is a novel nucleoid protein of E. coli under the anaerobic conditions, and thus propose to rename YgiP to Dan (DNA-binding protein under anaerobic conditions).
{"title":"Anaerobiosis-induced novel nucleoid protein of Escherichia coli: Architectural role in genome DNA compaction","authors":"J. Teramoto, Kayoko Yamada, N. Kobayashi, A. Kori, S. Yoshimura, K. Takeyasu, A. Ishihama","doi":"10.1109/MHS.2009.5351819","DOIUrl":"https://doi.org/10.1109/MHS.2009.5351819","url":null,"abstract":"A systematic search has been performed for DNA-binding sequences recognized by YgiP, an uncharacterized transcription factor of Escherichia coli, using the newly developed Genomic SELEX. By SELEX-clos procedure, a total of 333 sequences have been isolated from a total of 126 loci of the E. coli genome while more than 703 YgiP-binding loci have been identified after the genome-wide profiling by SELEX-chip procedure. Gel mobility shift assay indicated the presence of multiple YgiP-binding sites along each SELEX DNA fragment while DNase-I foot-printing assay indicated protection of short-specific sequences by YgiP, together suggesting YgiP as a nucleoid protein with broad specificity of DNA binding. Atomic force microscope (AFM) observation indicated that at low concentrations, YgiP binds to various sites in non-specific manner, but at high concentrations, YgiP covers the entire DNA surface. The intracellular level of YgiP was found to be very low in steady-state of cell growth under aerobic conditions, but increased more than 100-fold to the level as high as those of HU and IHF under hypoxic or anaerobic culture conditions. An E. coli mutant lacking ygiP showed abnormal growth under anaerobic conditions. Taken together we conclude that YgiP is a novel nucleoid protein of E. coli under the anaerobic conditions, and thus propose to rename YgiP to Dan (DNA-binding protein under anaerobic conditions).","PeriodicalId":344667,"journal":{"name":"2009 International Symposium on Micro-NanoMechatronics and Human Science","volume":"63 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2009-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116488104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-12-11DOI: 10.1109/MHS.2009.5352073
Akiyuki Hasegawa, H. Uvet, K. Ohara, T. Takubo, Y. Mae, T. Arai
Recently, micro valves to control some kinds of fluids in channels in a microchip have been developed. Now we pay attention to in-chip somatic cell cloning with microfluidic technology. There are some steps, cell supply, cutting, sorting, coupling, fusion and so on, for this work. And we focused on a cell supply system spending cells one by one in microfluidic chip, and attempt to transport an individual cell to objective course by the control of pressure valves. Here, we made three kinds valves, a) open channel 2-layer valve, b) open channel 3-layer valve, and c) close channel 2-layer valve. We compare these valves about interception condition of micro channel flow and time to take for open/close. And then we evaluated them with suitable for the smooth cell transportation by these comparisons.
{"title":"Micro valve system for individual cell transportation in microfluidic chip","authors":"Akiyuki Hasegawa, H. Uvet, K. Ohara, T. Takubo, Y. Mae, T. Arai","doi":"10.1109/MHS.2009.5352073","DOIUrl":"https://doi.org/10.1109/MHS.2009.5352073","url":null,"abstract":"Recently, micro valves to control some kinds of fluids in channels in a microchip have been developed. Now we pay attention to in-chip somatic cell cloning with microfluidic technology. There are some steps, cell supply, cutting, sorting, coupling, fusion and so on, for this work. And we focused on a cell supply system spending cells one by one in microfluidic chip, and attempt to transport an individual cell to objective course by the control of pressure valves. Here, we made three kinds valves, a) open channel 2-layer valve, b) open channel 3-layer valve, and c) close channel 2-layer valve. We compare these valves about interception condition of micro channel flow and time to take for open/close. And then we evaluated them with suitable for the smooth cell transportation by these comparisons.","PeriodicalId":344667,"journal":{"name":"2009 International Symposium on Micro-NanoMechatronics and Human Science","volume":"195 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2009-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114444742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-12-11DOI: 10.1109/MHS.2009.5352069
T. Iwami, T. Aizawa, K. Miyawaki, T. Matsunaga, Y. Shimada, G. Obinata
Recently, functional electrical stimulation (FES) has been applied for the treatment of the shoulder subluxation. The purpose of this study is to design a musculo-skeletal dynamic model for hemiplegic shoulder subluxation with which can perform FES effectively as well as the simulation of shoulder motion by FES. We installed the muscle models into the skeletal model. In this model, scapula and clavicle are fixed, and only gleno-humeral joint can move. We set up muscle activation, and stimulate deltoideus and supuraspinatus. As a result of simulation, we can confirm the reduction of shoulder subluxation.
{"title":"Model simulation of FES for the treatment of shoulder subluxation","authors":"T. Iwami, T. Aizawa, K. Miyawaki, T. Matsunaga, Y. Shimada, G. Obinata","doi":"10.1109/MHS.2009.5352069","DOIUrl":"https://doi.org/10.1109/MHS.2009.5352069","url":null,"abstract":"Recently, functional electrical stimulation (FES) has been applied for the treatment of the shoulder subluxation. The purpose of this study is to design a musculo-skeletal dynamic model for hemiplegic shoulder subluxation with which can perform FES effectively as well as the simulation of shoulder motion by FES. We installed the muscle models into the skeletal model. In this model, scapula and clavicle are fixed, and only gleno-humeral joint can move. We set up muscle activation, and stimulate deltoideus and supuraspinatus. As a result of simulation, we can confirm the reduction of shoulder subluxation.","PeriodicalId":344667,"journal":{"name":"2009 International Symposium on Micro-NanoMechatronics and Human Science","volume":"04 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2009-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134463205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-12-11DOI: 10.1109/MHS.2009.5352022
W. Jatmiko, Rochmatullah, B. Kusumoputro, H. Sanabila, K. Sekiyama, Toshio Fukuda
An electronic nose system had been developed by using 16 quartz resonator sensitive membranes-basic resonance frequencies 20 MHz as a sensor, and analyzed the measurement data through various neural network as a pattern recognition system. The developed system showed high recognition probability to discriminate various single odors even mixture odor to its high generality properties; however the system still need improvement. In order to improve the performance of the proposed system, development of the sensor and other neural network are being sought. This paper explains the improvement of the capability of that system from the point of neural network system. It has been proved from our previous work that FLVQ (Fuzzy Learning Vector Quantization) which is LVQ (Learning Vector Quantization) together with fuzzy theory shows high recognition capability compared with other neural networks, however FLVQ have a weakness for selecting the best codebook vector that will influence the result of recognition. This problem will be anticipated by adding the PSO (Particle Swarm Optimization) method to select the best codebook vector. Then experiment show that the new recognition system (FLVQ-PSO) has produced higher capability compared to the earlier mentioned system.
{"title":"Visualization and statistical analysis of fuzzy-neuro learning vector quantization based on particle swarm optimization for recognizing mixture odors","authors":"W. Jatmiko, Rochmatullah, B. Kusumoputro, H. Sanabila, K. Sekiyama, Toshio Fukuda","doi":"10.1109/MHS.2009.5352022","DOIUrl":"https://doi.org/10.1109/MHS.2009.5352022","url":null,"abstract":"An electronic nose system had been developed by using 16 quartz resonator sensitive membranes-basic resonance frequencies 20 MHz as a sensor, and analyzed the measurement data through various neural network as a pattern recognition system. The developed system showed high recognition probability to discriminate various single odors even mixture odor to its high generality properties; however the system still need improvement. In order to improve the performance of the proposed system, development of the sensor and other neural network are being sought. This paper explains the improvement of the capability of that system from the point of neural network system. It has been proved from our previous work that FLVQ (Fuzzy Learning Vector Quantization) which is LVQ (Learning Vector Quantization) together with fuzzy theory shows high recognition capability compared with other neural networks, however FLVQ have a weakness for selecting the best codebook vector that will influence the result of recognition. This problem will be anticipated by adding the PSO (Particle Swarm Optimization) method to select the best codebook vector. Then experiment show that the new recognition system (FLVQ-PSO) has produced higher capability compared to the earlier mentioned system.","PeriodicalId":344667,"journal":{"name":"2009 International Symposium on Micro-NanoMechatronics and Human Science","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2009-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133481635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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