Pub Date : 2009-12-11DOI: 10.1109/MHS.2009.5351919
Masayasu Suzuki, Hiroyuki Tanaka, Y. Iribe
This paper describes the single cell based detection and collection system based on respiration or metabolic activity. The micro-arrayed chemical sensors, which we have already developed, were used for parallel monitoring of single cell activity. These sensors consist of optical sensor film for pH or oxygen, and micro well array prepared with carbon black doped PDMS. We have succeeded in a parallel monitoring for single cell respiration activity by using a microarrayed oxygen sensor. We have also developed the automatic single cell collection instrument which can be equipped with a commercial inverted microscope. In this study, we have succeeded in the development of single cell based detection and collection system based on respiration or metabolic activity by combining these two technologies.
{"title":"Detection and collection system of target single cell based on respiration and metabolic activity","authors":"Masayasu Suzuki, Hiroyuki Tanaka, Y. Iribe","doi":"10.1109/MHS.2009.5351919","DOIUrl":"https://doi.org/10.1109/MHS.2009.5351919","url":null,"abstract":"This paper describes the single cell based detection and collection system based on respiration or metabolic activity. The micro-arrayed chemical sensors, which we have already developed, were used for parallel monitoring of single cell activity. These sensors consist of optical sensor film for pH or oxygen, and micro well array prepared with carbon black doped PDMS. We have succeeded in a parallel monitoring for single cell respiration activity by using a microarrayed oxygen sensor. We have also developed the automatic single cell collection instrument which can be equipped with a commercial inverted microscope. In this study, we have succeeded in the development of single cell based detection and collection system based on respiration or metabolic activity by combining these two technologies.","PeriodicalId":344667,"journal":{"name":"2009 International Symposium on Micro-NanoMechatronics and Human Science","volume":"54 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2009-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124800166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-12-11DOI: 10.1109/MHS.2009.5352076
H. Sasaki, N. Kubota, K. Sekiyama, T. Fukuda
Recently, various types of robots have been researched and developed for supporting our life. Also, the perceptual system for the robot is researched. Visual perception includes a lot of valuable information and it is useful for all intelligent robot system. In this paper, we discuss intelligent robot vision in order to detect multiple object and human. There are many visual sensors and we use the range-imaging camera to detect distance and image data. We propose a method for perceive moving target by using growing neural gas and Genetic algorithm. In the experimental results, we show the potency of our method.
{"title":"Multiple object detection for intelligent robot vision by using growing neural gas","authors":"H. Sasaki, N. Kubota, K. Sekiyama, T. Fukuda","doi":"10.1109/MHS.2009.5352076","DOIUrl":"https://doi.org/10.1109/MHS.2009.5352076","url":null,"abstract":"Recently, various types of robots have been researched and developed for supporting our life. Also, the perceptual system for the robot is researched. Visual perception includes a lot of valuable information and it is useful for all intelligent robot system. In this paper, we discuss intelligent robot vision in order to detect multiple object and human. There are many visual sensors and we use the range-imaging camera to detect distance and image data. We propose a method for perceive moving target by using growing neural gas and Genetic algorithm. In the experimental results, we show the potency of our method.","PeriodicalId":344667,"journal":{"name":"2009 International Symposium on Micro-NanoMechatronics and Human Science","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2009-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127389893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-12-11DOI: 10.1109/MHS.2009.5351837
M. Horade, S. Sugiyama
Research on establishment of a fabrication method of three-dimensional microstructure which used SR (Synchrotron Radiation) light is reported. As the advantage of this method, since there is no necessity of fabrication masks and it is suitable for rapid prototyping, reduction of fabrication time and cost is mentioned. In this time, we carry out basic research about realization of this fabrication method, and it succeeded in fabrication of the free-form surface by exposure between pixels overlap. Moreover, in order to fabricate the three-dimensional structure of arbitrary shape, the algorithm which can determine exposure energy amount in consideration of overlap was written to target form. Using this algorithm, fabrication of various arbitrary three-dimensional structures was possible by only single aperture without manufacture a mask for every form. It is examining using this technique for fabrication of μ-TAS or a reactor. The channel which has the inclination and free-form surface are fabricated by this method, improvement in functionality is promising.
{"title":"Study on optimization of exposure energy distribution for fabrication of arbitrary 3-D microstructure by shaped beam using synchrotron radiation","authors":"M. Horade, S. Sugiyama","doi":"10.1109/MHS.2009.5351837","DOIUrl":"https://doi.org/10.1109/MHS.2009.5351837","url":null,"abstract":"Research on establishment of a fabrication method of three-dimensional microstructure which used SR (Synchrotron Radiation) light is reported. As the advantage of this method, since there is no necessity of fabrication masks and it is suitable for rapid prototyping, reduction of fabrication time and cost is mentioned. In this time, we carry out basic research about realization of this fabrication method, and it succeeded in fabrication of the free-form surface by exposure between pixels overlap. Moreover, in order to fabricate the three-dimensional structure of arbitrary shape, the algorithm which can determine exposure energy amount in consideration of overlap was written to target form. Using this algorithm, fabrication of various arbitrary three-dimensional structures was possible by only single aperture without manufacture a mask for every form. It is examining using this technique for fabrication of μ-TAS or a reactor. The channel which has the inclination and free-form surface are fabricated by this method, improvement in functionality is promising.","PeriodicalId":344667,"journal":{"name":"2009 International Symposium on Micro-NanoMechatronics and Human Science","volume":"70 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2009-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128880892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-12-11DOI: 10.1109/MHS.2009.5352095
K. Ooe, T. Fukuda
During hemanalysis, it is necessary to separate blood cells from whole blood. Many blood separation methods, for example, centrifugation and filtering, are in practical use. However, the use of these methods involves problems from the perspectives of processing speed and processing volume. We develop new types of blood separation devices that use piezo-ceramic vibrators. The first device uses a capillary. One end of the capillary is fixed to the device frame, and the other is fixed to a piezo-ceramic vibrator. The vibrator transmits bending waves to the capillary. This device can process only a small amount of solution; therefore, it is not suitable for hemanalysis. In order to solve this problem, we developed a second device; this device has a pair of thin glass plates with a small gap as a substitute for the capillary used in the first device. These devices are based on the fact that particles heavier than water move toward transverse velocity antinodes while those lighter than water move toward velocity nodes. In this report, we demonstrate the high-speed separation of silica microbeads and 50-vol% glycerol water by using these devices. The first device can separate the abovementioned solution within 3 min while the second can separate it within 1 min. Both devices are driven by a rectangular wave of 15 to 20 Vpp. Furthermore, it has been confirmed that red blood cells are separated from diluted whole blood using the first device within approximately 1 min. These devices have transparency, so they can compose as the analysis system with the chemical analyzer easily.
{"title":"Development of micro particles separation device with piezo-ceramic vibrator","authors":"K. Ooe, T. Fukuda","doi":"10.1109/MHS.2009.5352095","DOIUrl":"https://doi.org/10.1109/MHS.2009.5352095","url":null,"abstract":"During hemanalysis, it is necessary to separate blood cells from whole blood. Many blood separation methods, for example, centrifugation and filtering, are in practical use. However, the use of these methods involves problems from the perspectives of processing speed and processing volume. We develop new types of blood separation devices that use piezo-ceramic vibrators. The first device uses a capillary. One end of the capillary is fixed to the device frame, and the other is fixed to a piezo-ceramic vibrator. The vibrator transmits bending waves to the capillary. This device can process only a small amount of solution; therefore, it is not suitable for hemanalysis. In order to solve this problem, we developed a second device; this device has a pair of thin glass plates with a small gap as a substitute for the capillary used in the first device. These devices are based on the fact that particles heavier than water move toward transverse velocity antinodes while those lighter than water move toward velocity nodes. In this report, we demonstrate the high-speed separation of silica microbeads and 50-vol% glycerol water by using these devices. The first device can separate the abovementioned solution within 3 min while the second can separate it within 1 min. Both devices are driven by a rectangular wave of 15 to 20 Vpp. Furthermore, it has been confirmed that red blood cells are separated from diluted whole blood using the first device within approximately 1 min. These devices have transparency, so they can compose as the analysis system with the chemical analyzer easily.","PeriodicalId":344667,"journal":{"name":"2009 International Symposium on Micro-NanoMechatronics and Human Science","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2009-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129845717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-12-11DOI: 10.1109/MHS.2009.5351824
N. Inomata, T. Mizunuma, Y. Yamanishi, S. Kudo, F. Arai
We developed a magnetically driven microtool(MMT) used in a microfluidic chip for enucleation of oocyte. To achieve technological innovation, weight of the tool is about 1/300,000 compared with that of the conventional mechanical micromanipulator and was installed in a chip. We succeeded in precise positioning of the tool (5 μm) with low disturbances. The tool is actuated noncontact by the magnetic force, therefore, the microfluidic chip part is fully disposable with low cost. Of special notes are following. (1) Novel vibrating type of MMT is proposed to reduce the dead band of the magnetic actuation. (2) Omni-directional actuation of the tool was achieved by controlling magnetic field focused on a chip. (3) Backlash of the tool was reduced by supporting it by the flexible hinge with isotropic spring constant. (4) A polymer-metal hybrid structure which has properties of both elasticity and rigidity was employed for the tool. (5) Based on the novel and original design, we integrated a Robot-on-a-Chip (Robochip) and demonstrated on-chip enucleation of oocyte.
{"title":"On-chip magnetically driven micro-robot for enucleation of oocyte","authors":"N. Inomata, T. Mizunuma, Y. Yamanishi, S. Kudo, F. Arai","doi":"10.1109/MHS.2009.5351824","DOIUrl":"https://doi.org/10.1109/MHS.2009.5351824","url":null,"abstract":"We developed a magnetically driven microtool(MMT) used in a microfluidic chip for enucleation of oocyte. To achieve technological innovation, weight of the tool is about 1/300,000 compared with that of the conventional mechanical micromanipulator and was installed in a chip. We succeeded in precise positioning of the tool (5 μm) with low disturbances. The tool is actuated noncontact by the magnetic force, therefore, the microfluidic chip part is fully disposable with low cost. Of special notes are following. (1) Novel vibrating type of MMT is proposed to reduce the dead band of the magnetic actuation. (2) Omni-directional actuation of the tool was achieved by controlling magnetic field focused on a chip. (3) Backlash of the tool was reduced by supporting it by the flexible hinge with isotropic spring constant. (4) A polymer-metal hybrid structure which has properties of both elasticity and rigidity was employed for the tool. (5) Based on the novel and original design, we integrated a Robot-on-a-Chip (Robochip) and demonstrated on-chip enucleation of oocyte.","PeriodicalId":344667,"journal":{"name":"2009 International Symposium on Micro-NanoMechatronics and Human Science","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2009-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129872618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-12-11DOI: 10.1109/MHS.2009.5351836
M. Gel, Y. Mori, Y. Kimura, O. Kurosawa, B. Techaumnat, H. Oana, M. Washizu
Micro orifice assisted cell fusion assures high-yield fusion without compromising the cell viability. This paper examines feasibility of a cell pairing method compatible with micro orifice based cell fusion to create large number of viable fusants for studying post-fusion cell behavior. We fabricated a microfluidic chip which contained a chamber and a partition. The partition divided the chamber into two compartments and it had a number of embedded micro orifices. The voltage applied to the electrodes located at each compartment generated electric field distribution concentrating in each micro orifice. Cells introduced into each compartment moved towards micro orifice by manipulation of hydrostatic pressure. Dielectrophoretic force trapped the cells in micro orifice and established cell to cell contact through orifice. By applying a pulse, cell fusion was initiated to form a neck between cells. The neck passing through orifice resulted in immobilization of the fused cell pair. Unfused cells washed away. Then, the chip was loaded to a microscope stage incubator for time lapse imaging of the immobilized fusants. The viable fusants were successfully generated by fusion of mouse fibroblast cells. Time lapse observation of the five fusants showed that fused cell pairs were released from micro orifice and became one body. Fusants which reached to cell division phase divided into three daughter cells. We conclude that the presented method of cell pairing and fusion is suitable for high-yield generation of viable fusants and studying of post-fusion phenomena.
{"title":"Micro orifice based cell pairing and fusion on microfluidic chip","authors":"M. Gel, Y. Mori, Y. Kimura, O. Kurosawa, B. Techaumnat, H. Oana, M. Washizu","doi":"10.1109/MHS.2009.5351836","DOIUrl":"https://doi.org/10.1109/MHS.2009.5351836","url":null,"abstract":"Micro orifice assisted cell fusion assures high-yield fusion without compromising the cell viability. This paper examines feasibility of a cell pairing method compatible with micro orifice based cell fusion to create large number of viable fusants for studying post-fusion cell behavior. We fabricated a microfluidic chip which contained a chamber and a partition. The partition divided the chamber into two compartments and it had a number of embedded micro orifices. The voltage applied to the electrodes located at each compartment generated electric field distribution concentrating in each micro orifice. Cells introduced into each compartment moved towards micro orifice by manipulation of hydrostatic pressure. Dielectrophoretic force trapped the cells in micro orifice and established cell to cell contact through orifice. By applying a pulse, cell fusion was initiated to form a neck between cells. The neck passing through orifice resulted in immobilization of the fused cell pair. Unfused cells washed away. Then, the chip was loaded to a microscope stage incubator for time lapse imaging of the immobilized fusants. The viable fusants were successfully generated by fusion of mouse fibroblast cells. Time lapse observation of the five fusants showed that fused cell pairs were released from micro orifice and became one body. Fusants which reached to cell division phase divided into three daughter cells. We conclude that the presented method of cell pairing and fusion is suitable for high-yield generation of viable fusants and studying of post-fusion phenomena.","PeriodicalId":344667,"journal":{"name":"2009 International Symposium on Micro-NanoMechatronics and Human Science","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2009-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122577923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-12-11DOI: 10.1109/MHS.2009.5351981
Y. Akiyama, K. Fukumori, M. Yamato, K. Sakai, T. Okano
Ultra thin temperature responsive polymer, poly(N-isopropylacrylamide) (PIPAAm) grafted layer exhibits cell attachment and detachment properties in response to temperature change. Such properties are dependent on thickness and amount of the grafted PIPAAm, and are observed for extremely ultra thin thickness (for example, 20 nm thickness for tissue culture poly styrene and 5 nm for glass cover slips). Although this phenomenon was not demonstrated clearly, we have proposed a mechanism. Namely, thinner PIPAAm layer are subjected to dehydration of the PIPAAm chains in the vicinity of basal surfaces. The dehydration progressively promotes dehydration of the grafted PIPAAm chains toward to the outermost regions. In this presentation, we tried to demonstrate the proposed mechanism by investigating the dynamic alternation of thickness of PIPAAm layer in response to temperature. The alternation was measured undere aqueous conditions above and below LCST, using AFM. As a result, thickness of thinner PIPAAm layer was not altered between under atmospheric and aqueous conditions, while thicker layer was done. This difference strongly support the proposed mechanism.
{"title":"Features of ultra thin poly(N-isopropylacrylamide) grafted onto glass cover slips","authors":"Y. Akiyama, K. Fukumori, M. Yamato, K. Sakai, T. Okano","doi":"10.1109/MHS.2009.5351981","DOIUrl":"https://doi.org/10.1109/MHS.2009.5351981","url":null,"abstract":"Ultra thin temperature responsive polymer, poly(N-isopropylacrylamide) (PIPAAm) grafted layer exhibits cell attachment and detachment properties in response to temperature change. Such properties are dependent on thickness and amount of the grafted PIPAAm, and are observed for extremely ultra thin thickness (for example, 20 nm thickness for tissue culture poly styrene and 5 nm for glass cover slips). Although this phenomenon was not demonstrated clearly, we have proposed a mechanism. Namely, thinner PIPAAm layer are subjected to dehydration of the PIPAAm chains in the vicinity of basal surfaces. The dehydration progressively promotes dehydration of the grafted PIPAAm chains toward to the outermost regions. In this presentation, we tried to demonstrate the proposed mechanism by investigating the dynamic alternation of thickness of PIPAAm layer in response to temperature. The alternation was measured undere aqueous conditions above and below LCST, using AFM. As a result, thickness of thinner PIPAAm layer was not altered between under atmospheric and aqueous conditions, while thicker layer was done. This difference strongly support the proposed mechanism.","PeriodicalId":344667,"journal":{"name":"2009 International Symposium on Micro-NanoMechatronics and Human Science","volume":"28 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2009-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122809276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-12-11DOI: 10.1109/MHS.2009.5351973
R. Amarasinghe, D. Dao, S. Sugiyama
This study presents the design, fabrication and characterization of an ultra miniaturized 3-axis accelerometer with implanted piezoresistive sensing elements and read out circuits are having nano-scale dimensions. It has been developed using MEMS/NEMS machining and fabrication techniques. This sensor consists of a new sub-millimeter structure with seismic mass and combined cross-beam and surrounding beams. It can detect three components of linear acceleration simultaneously. The sensitivity could be enhanced significantly while miniaturizing the die size of sensor chip with aid of novel structure and nanoscale piezoresistors on the sensing beams. Therefore, this novel proposed sensor is showing good performance and smaller than other comparable miniaturized sensor structures reported thus far. The accelerometer is capable of measuring accelerations up to ±20g in the frequency bandwidth of 480Hz. Comparison of the obtained experimental results and finite element simulation shows good agreement. This accelerometer is being introduced for a wearable physical activity monitoring systems.
{"title":"Ultra miniature µ-accelerometer for wearable physical activity monitoring systems","authors":"R. Amarasinghe, D. Dao, S. Sugiyama","doi":"10.1109/MHS.2009.5351973","DOIUrl":"https://doi.org/10.1109/MHS.2009.5351973","url":null,"abstract":"This study presents the design, fabrication and characterization of an ultra miniaturized 3-axis accelerometer with implanted piezoresistive sensing elements and read out circuits are having nano-scale dimensions. It has been developed using MEMS/NEMS machining and fabrication techniques. This sensor consists of a new sub-millimeter structure with seismic mass and combined cross-beam and surrounding beams. It can detect three components of linear acceleration simultaneously. The sensitivity could be enhanced significantly while miniaturizing the die size of sensor chip with aid of novel structure and nanoscale piezoresistors on the sensing beams. Therefore, this novel proposed sensor is showing good performance and smaller than other comparable miniaturized sensor structures reported thus far. The accelerometer is capable of measuring accelerations up to ±20g in the frequency bandwidth of 480Hz. Comparison of the obtained experimental results and finite element simulation shows good agreement. This accelerometer is being introduced for a wearable physical activity monitoring systems.","PeriodicalId":344667,"journal":{"name":"2009 International Symposium on Micro-NanoMechatronics and Human Science","volume":"115 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2009-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131541370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-12-11DOI: 10.1109/MHS.2009.5351929
Takuro Takahata, S. Kume, H. Miyoshi, T. Sugiura, A. Honda
Influenza virus infection induced various changing of the host cell. At the beginning of influenza virus infection, the transcription from viral genome RNA by RNA-dependent RNA polymerase took place by cleavage of host capped RNA using own endonuclease for priming of mRNA synthesis. After the viral protein NS1 expression, NS1 binds to CPSF lead to inhibit polyA addition of host mRNA (1). Finally the influenza virus infection reduced host cell protein expression. While after viral genome transcription, viral membrane protein HA, NA and M1 expressed and localized on the cellular membrane. It is thought that this phenomenon induced the cellular membrane stiffness. We performed to measure the stiffness of cellular membrane using optical laser and collagen coated-beads. Interestingly at 4 hrs after influenza virus infection, the membrane stiffness changed and also the level of integrin in the cellular membrane was decreased.
{"title":"Analysis of cellular membrane changing induced by influenza virus infection","authors":"Takuro Takahata, S. Kume, H. Miyoshi, T. Sugiura, A. Honda","doi":"10.1109/MHS.2009.5351929","DOIUrl":"https://doi.org/10.1109/MHS.2009.5351929","url":null,"abstract":"Influenza virus infection induced various changing of the host cell. At the beginning of influenza virus infection, the transcription from viral genome RNA by RNA-dependent RNA polymerase took place by cleavage of host capped RNA using own endonuclease for priming of mRNA synthesis. After the viral protein NS1 expression, NS1 binds to CPSF lead to inhibit polyA addition of host mRNA (1). Finally the influenza virus infection reduced host cell protein expression. While after viral genome transcription, viral membrane protein HA, NA and M1 expressed and localized on the cellular membrane. It is thought that this phenomenon induced the cellular membrane stiffness. We performed to measure the stiffness of cellular membrane using optical laser and collagen coated-beads. Interestingly at 4 hrs after influenza virus infection, the membrane stiffness changed and also the level of integrin in the cellular membrane was decreased.","PeriodicalId":344667,"journal":{"name":"2009 International Symposium on Micro-NanoMechatronics and Human Science","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2009-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122957094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-12-11DOI: 10.1109/MHS.2009.5352051
H. Oana, A. Kishimura, Y. Yamasaki, M. Washizu, K. Kataoka
Spontaneous formation of a giant polymer vesicle from a single micrometer-sized droplet of polyion complex (PIC) of diblock copolymers and its derivative by thermal perturbation, which is achieved by irradiation with a focused infrared laser is presented. The thermal perturbation induces a microphase separation inside of the PIC droplet and the generated water rich phase in the PIC droplet becomes a content of the vesicle and the PIC is deformed into a self-assembled membrane of the vesicle. The size of the giant unilamellar vesicles formed is determined on the basis of the initial size of the PIC droplets.
{"title":"Spontaneous formation of giant unilamellar vesicles from microdroplets of a polyion complex by focused infrared laser irradiation","authors":"H. Oana, A. Kishimura, Y. Yamasaki, M. Washizu, K. Kataoka","doi":"10.1109/MHS.2009.5352051","DOIUrl":"https://doi.org/10.1109/MHS.2009.5352051","url":null,"abstract":"Spontaneous formation of a giant polymer vesicle from a single micrometer-sized droplet of polyion complex (PIC) of diblock copolymers and its derivative by thermal perturbation, which is achieved by irradiation with a focused infrared laser is presented. The thermal perturbation induces a microphase separation inside of the PIC droplet and the generated water rich phase in the PIC droplet becomes a content of the vesicle and the PIC is deformed into a self-assembled membrane of the vesicle. The size of the giant unilamellar vesicles formed is determined on the basis of the initial size of the PIC droplets.","PeriodicalId":344667,"journal":{"name":"2009 International Symposium on Micro-NanoMechatronics and Human Science","volume":"122 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2009-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124618066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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