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2009 International Symposium on Micro-NanoMechatronics and Human Science最新文献

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Evaluation of human sense by biological information analysis 用生物信息分析评价人的感官
Pub Date : 2009-12-11 DOI: 10.1109/MHS.2009.5352071
Xiaojie Zhuang, K. Sekiyama, T. Fukuda
In this paper, we present the evaluation of human's emotion by using brain waves. As an honest characteristic of fluctuating of human's emotion, EEG electroencephalography (EEG) has been studied by many researchers these years. P300 as an important endogenous component of event-related potential (ERP), affects people's concentration of attention. In general EEG researches, comfortableness was always concerned, while achievement and nervousness was rarely reported. In this paper, we try to measure and compare P300 by achievement stimulus and nervousness stimulus. The experiment is a game called “Finding Difference”, which focuses on the visual irritation. By “Signal Averaging”, we abstract the ERP, and studied the appearance of P300 (P: positive, 300: latency of 300ms), and estimate the concentration stimulated by achievement and nervousness. As the result, we found that in the case of nervousness stimulus, P300 appeared more apparently than that in the case of achievement stimulus.
本文提出了利用脑电波对人的情感进行评价的方法。脑电图作为人类情绪波动的一个真实特征,近年来受到了许多研究者的研究。P300作为事件相关电位(event-related potential, ERP)的重要内源性成分,影响人的注意力集中。在一般的脑电图研究中,对舒适性的研究较多,而对成就性和紧张性的研究较少。本文尝试用成就刺激和紧张刺激来测量和比较P300。这个实验是一个名为“发现差异”的游戏,主要关注视觉刺激。通过“信号平均”提取ERP,研究P300 (P:正,300:潜伏期300ms)的出现,并估计成就和紧张刺激下的注意力。结果发现,在紧张刺激下,P300的出现比在成就刺激下更为明显。
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引用次数: 5
Reconstitution of biological clock into phospholipid-coated microdroplet 磷脂包被微滴的生物钟重构
Pub Date : 2009-12-11 DOI: 10.1109/MHS.2009.5351831
M. Kojima, M. Ohno, M. Nakajima, M. Homma, K. Takiguchi, T. Kondo, T. Fukuda
In this paper, we try to establish newly technique that the components of the biological clock are reconstituted into the liposome. In other words, we try to produce a nano size clock, capsulated into the liposome, made by protein molecules. The biological clock is a basic cellular system found in almost all organisms. This clock generates self-sustained oscillations under constant conditions with a ^24-hour (circadian) period. Only three proteins in cyanobacteria reconstituted biological clock. We reconstitute these proteins and adenosine triphosphate (ATP) into phospholipid-coated microdroplet and confirmed the clock function. This system will reveal more detailed about biological clock system and bring application possibility, such as drug delivery.
在本文中,我们试图建立一种新的技术,将生物钟的组成部分重组到脂质体中。换句话说,我们试图制造一个纳米大小的时钟,被包裹在脂质体中,由蛋白质分子制成。生物钟是几乎所有生物体中都存在的基本细胞系统。这种时钟在恒定条件下以24小时(昼夜节律)周期产生自我持续振荡。蓝藻中只有3种蛋白质重建了生物钟。我们将这些蛋白和三磷酸腺苷(ATP)重组成磷脂包被微滴,并证实了其时钟功能。该系统将更详细地揭示生物钟系统,并带来药物输送等应用的可能性。
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引用次数: 0
Controlling the higher-order structure of DNA-protamine complex 控制dna -鱼精蛋白复合物的高阶结构
Pub Date : 2009-12-11 DOI: 10.1109/MHS.2009.5351918
Y. Yoshikawa, N. Makita, Mari Suzuki, Eri Shindo, Chika Watanabe, T. Kanai, T. Imanaka, T. Kanbe, K. Yoshikawa
We investigated the interaction of large DNA with protamine, a small basic protein, using fluorescence microscopy and electron microscopy. In most vertebrate sperm cells, a large DNA is packaged by protamine into a highly compact, biologically inactive form of chromatin. The manner of packaging plays a key role in spermatogenesis. We found that, with increase of protamine concentration, DNA molecules show the conformational change from an elongated coil state into a compact state through the occurrence of the intermediate state. We also show that the higher-order structure of DNA-protamine complex can be controlled by optical tweezers (Nd YAG laser, 1064 nm) without any structural modification of DNA such as an attachment of plastic beads. These results are discussed in relation to the biological meaning.
我们利用荧光显微镜和电子显微镜研究了大DNA与小碱性蛋白鱼精蛋白的相互作用。在大多数脊椎动物的精子细胞中,一个大的DNA被鱼精蛋白包装成一个高度紧密的,生物无活性的染色质形式。包装方式在精子发生中起着关键作用。我们发现,随着鱼精蛋白浓度的增加,DNA分子通过中间态的发生,呈现出由细长线圈态向致密态的构象变化。我们还证明了DNA-鱼精蛋白复合物的高阶结构可以用光镊(Nd YAG激光,1064 nm)控制,而不需要对DNA进行任何结构修饰,如塑料珠的附着。这些结果在生物学意义上进行了讨论。
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引用次数: 0
Assembly of functional nanodebice using platinum/ tungsten nanowire 用铂/钨纳米线组装功能纳米器件
Pub Date : 2009-12-11 DOI: 10.1109/MHS.2009.5352025
Zhan Yang, M. Nakajima, T. Fukuda
This paper presets the assembly of a functional device with double metal nanowire. A PZT cantilever was used for electrodes of the device and etched by FIB. Two carbon nanotubes (CNT) were assembled to the separated electrodes of PZT cantilever. A tungsten probe is etched by focused ion beam (FIB) into 300 nm in diameter and 25.4 μm in length. Then the probe is coated by parylene and cut the tip to open the tungsten. A tungsten nanowire with 907nm in length and a platinum nanowire with 209 nm in length grew from the tip of the CNTs via field emission by introducing hexacabonyl tungsten and trimethyl cyclopentadienyl platinum individually inside a field emission electron microscope.
本文采用双金属纳米线预制了一种功能器件。电极采用PZT悬臂梁,经FIB刻蚀。将两个碳纳米管(CNT)组装在PZT悬臂梁的分离电极上。利用聚焦离子束(FIB)将钨探针蚀刻成直径为300 nm、长度为25.4 μm的材料。然后在探针上涂上一层聚对二甲苯,并切割尖端以打开钨。在场发射电子显微镜下,分别引入六加羰基钨和三甲基环戊二烯基铂,通过场发射从CNTs的尖端生长出长度为907nm的钨纳米线和长度为209 nm的铂纳米线。
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引用次数: 0
A viscous micropump using a spinning microrotor driven by a Laguerre-Gaussian beam 使用拉盖尔-高斯光束驱动的旋转微转子的粘性微泵
Pub Date : 2009-12-11 DOI: 10.1109/MHS.2009.5351924
S. Maruo, Yohei Saito
We proposed a novel viscous micropump driven by a Laguerre-Gaussian beam (LG beam). The micropump consists of a U-shaped microchannel and a microrotor. In this micropump, the rotation of the microrotor enables to transport fluid by use of the viscous drag surrounding the microrotor. To rotate the microrotor, we developed an optical manipulation system using a LG beam. Since LG beam has orbital angular momentum, it enables to rotate multiple microparticles along its doughnut-ring-like transversal intensity distribution. We examined the dependence of the rotation of multiple microparticles on topological charge l of LG beam. In our preliminary experiments, the highest rotation speed of 240 rpm with a rotation radius of 7.5 µm was obtained with a LG beam with topological charge l = 16. According to the result, we designed and fabricated LG beam driven microrotors of radius of 7.5 µm by using two-photon microfabrication. By optimizing the thickness of the microrotor, a microrotor driven at 240 rpm was produced by two-photon microfabrication. Finally, viscous micropump using the high-speed microrotor was developed.
提出了一种由拉盖尔-高斯光束驱动的新型粘性微泵。微泵由u型微通道和微转子组成。在这种微泵中,微转子的旋转使流体能够利用微转子周围的粘性阻力进行输送。为了旋转微转子,我们开发了一种使用LG光束的光学操纵系统。由于LG光束具有轨道角动量,它可以使多个微粒沿着其甜甜圈状的横向强度分布旋转。我们研究了多个微粒的旋转对LG束拓扑电荷l的依赖性。在我们的初步实验中,在拓扑电荷l = 16的LG光束下,获得了240 rpm的最高旋转速度,旋转半径为7.5µm。在此基础上,采用双光子微加工技术设计并制作了半径为7.5µm的LG光束驱动微转子。通过优化微转子的厚度,采用双光子微加工技术制备了转速为240 rpm的微转子。最后,研制了基于高速微转子的粘性微泵。
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引用次数: 1
The development of upper limbs rehabilitation robot system based on the difference of motor performance 基于运动性能差异的上肢康复机器人系统的研制
Pub Date : 2009-12-11 DOI: 10.1109/MHS.2009.5351839
Keunyoung Park, Youngwook Kim, G. Obinata
In this paper, we develop the robotic system for the rehabilitation of the upper limbs which consists of a PA-10 robot manipulator and a 6-axis force/torque sensor. In addition, we generate FES signals to assist paralytics based on the difference of reaching performance between normal and abnormal arms. To estimate the motor performance, subjects were asked to perform a tracking task; in this study, the healthy subjects conduct experiments. We verify that the FES signal values are saturating followed by the trial number and adjusted by reaching performance.
在本文中,我们开发了由PA-10机械手和6轴力/扭矩传感器组成的上肢康复机器人系统。此外,我们根据正常和异常手臂之间的到达性能差异产生FES信号来辅助瘫痪者。为了评估运动表现,受试者被要求执行跟踪任务;在本研究中,健康受试者进行实验。我们验证了FES信号值是饱和的,然后是试验数,并通过达到性能来调整。
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引用次数: 2
G protein coupled receptors (GPCRs) reconstituted on recombinant proteoliposomes using baculovirus-liposome membrane fusion 杆状病毒-脂质体膜融合技术在重组蛋白脂质体上重组G蛋白偶联受体(gpcr)
Pub Date : 2009-12-11 DOI: 10.1109/MHS.2009.5351994
K. Tsumoto, K. Kamiya, Sayaka Kitaoka, S. Ogata, M. Tomita, T. Yoshimura
Transmembrane proteins are important in biological functions. Proteoliposomes, which contain reconstituted membrane proteins, have been considered to be useful for their investigation. Especially, cell-sized giant liposomes, or giant unilamellar vesicles, are often used for such purposes. Previously, we established a novel method of proteoliposome preparation using membrane fusion between recombinant baculovirus and liposomes. Here we demonstrated preparation of recombinant proteoliposomes containing a G protein coupled receptor (GPCR) using this method. Human corticotropin releasing hormone receptor 1 (CRHR1), which is a seven-span transmembrane protein, was expressed using a baculovirus/insect cell recombinant system, and it was verified that the proteins were localized on both the insect cell membranes and baculovirus budded virus envelopes. The budded viruses were fused with GUVs containing DOPC/DOPS at pH ~4.5. The resulting proteo-GUVs were visualized using phycoerythrin-conjugated anti-CRHR antibodies. The CRHR1 recombinant proteoliposomes also reacted with anti-ligand antibodies in the presence of its ligand (corticotropin releasing factor). These results suggest that GPCRs can be reconstituted on proteoliposomes with an intact (native) function and structure using the baculovirus-liposome membrane fusion method.
跨膜蛋白具有重要的生物学功能。含有重组膜蛋白的蛋白脂质体被认为对它们的研究是有用的。特别是细胞大小的巨型脂质体,或巨大的单层囊泡,通常用于这种目的。在此之前,我们建立了一种利用重组杆状病毒与脂质体之间的膜融合制备蛋白脂质体的新方法。在这里,我们展示了用这种方法制备含有G蛋白偶联受体(GPCR)的重组蛋白脂质体。人促肾上腺皮质激素释放激素受体1 (CRHR1)是一个七跨跨膜蛋白,用杆状病毒/昆虫细胞重组系统表达,证实该蛋白既定位于昆虫细胞膜上,也定位于杆状病毒出芽包膜上。将出芽病毒与含有DOPC/DOPS的guv在pH ~4.5下融合。利用植红蛋白偶联抗crhr抗体对生成的蛋白- guv进行可视化。CRHR1重组蛋白脂质体也在其配体(促肾上腺皮质激素释放因子)存在的情况下与抗配体抗体发生反应。这些结果表明,使用杆状病毒-脂质体膜融合方法可以在蛋白脂质体上重组具有完整(天然)功能和结构的gpcr。
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引用次数: 3
Development of a semi-autonomous remote maintenance robot 半自主远程维修机器人的研制
Pub Date : 2009-12-11 DOI: 10.1109/MHS.2009.5352006
Akihiro Hibino, Tatsuya Kawai, Mikio Nako, Yingxin He, Kazuki Aoyama, Yuta Iwata, Taisuke Isogai, S. Takeuchi, K. Tatsuno
We are developing a proto-type of semi-autonomous remote maintenance robot which is for example used in a nuclear power plant. The image of the robot is shown in Fig.1. The robot is constructed of arms, mobile base and vision system. The robot is operated with task level instructions. Foe example, when we direct “Insert the bolt”, the robot search the instruction file in which the move sequences are described, and then the task is performed by moving the mobile base and arms.
我们正在开发一种半自动远程维护机器人的原型,例如用于核电站。机器人图像如图1所示。该机器人由手臂、移动基座和视觉系统组成。机器人是按照任务级指令操作的。例如,当我们指令“插入螺栓”时,机器人搜索描述移动序列的指令文件,然后通过移动移动基座和手臂来执行任务。
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引用次数: 0
Examination of high luminance light guide plate by accumulating method 累积法检测高亮度导光板
Pub Date : 2009-12-11 DOI: 10.1109/MHS.2009.5352103
Takaya Fujimoto, Y. Okayama, K. Yamashita, S. Nishida, Y. Sawa, D. Noda, T. Hattori
LEDs are attracting attention as a light source for a new form of lighting, taking the place of the fluorescent lamp and the light bulb. They are not suitable for lighting use by themselves, as LEDs are a point light source. However, it is possible to convert them into plane emission form by combining them with light guide plate technology used for the liquid crystal displays. It is therefore possible to use them as lighting devices through the plane emission method. In this laboratory, a minute dot was formed at the bottom of a light guide plate, and light hitting the dot was emitted from the luminescence side of the light guide plate. The amount of light emitted from the luminescence side also increased, if the number of these dots was increased from previous research results. It was thought that a high luminance plate or a plate guiding more light could be created. A method of piling thin light guide plates was proposed as a method of increasing the number of dots, with the piled light guide plates being designed using an optical simulator.
led作为一种取代荧光灯和灯泡的新型照明光源,正受到人们的关注。它们不适合单独照明,因为led是点光源。然而,将其与用于液晶显示器的导光板技术相结合,可以将其转化为平面发射形式。因此,可以通过平面发射法将它们用作照明装置。本实验室在导光板的底部形成一个小点,光从导光板的发光侧照射到小点上。如果从先前的研究结果中增加这些点的数量,那么从发光侧发出的光量也会增加。人们认为可以制造出高亮度板或引导更多光的板。提出了一种叠薄导光板的方法来增加光斑的数量,并利用光学模拟器对叠薄导光板进行了设计。
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引用次数: 0
Intelligent gels — An approach to artificial muscles and soft tissue 智能凝胶——人造肌肉和软组织的一种方法
Pub Date : 2009-12-11 DOI: 10.1109/MHS.2009.5352100
Y. Osada, Gong Jian Ping
We have been employing polymer gels to create biomimetic motility systems, focusing to their reversible size and shape change, thereby realizing motion by integrating the deformation on a molecular level. Over the past number of years, we have proposed several kinds of artificial soft machines constructed by polymer gels. Examples include Gelooper(gel-looper), gelf(gel golf) [1–6], shape memory gel actuators[7–10], chemical motors[11,12], etc.
我们一直在使用聚合物凝胶来创建仿生运动系统,专注于其可逆的大小和形状变化,从而通过在分子水平上整合变形来实现运动。在过去的几年里,我们提出了几种由聚合物凝胶构成的人造软体机器。例如Gelooper(gel-looper)、golf (gel golf)[1-6]、形状记忆凝胶致动器[7-10]、化学马达[11,12]等。
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引用次数: 0
期刊
2009 International Symposium on Micro-NanoMechatronics and Human Science
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