Pub Date : 2022-12-12DOI: 10.31043/2410-2733-2022-3-92-98
U. Berezina, M. Kochurnikova, O. Bespyatykh, I. Domskiy
Purpose: The study of biochemical indicators of blood of females and males of fox hybrids with age.Materials and methods. Research was carried out on females and males of fox hybrids. The age dynamics of changes in the biochemical parameters of the blood was evaluated with a comparative analysis of 2 age groups: females and males of fox hybrids 2 and 5 months (n = 30). The animals were fed once a day (in the morning) with meat mixtures in accordance with age and physiological condition, contained under the same conditions. Blood from the animals was taken into a special test tube with an activator of a clot of lateral subcutaneous vein of the lower leg until morning feeding. They received serum from it by centrifugation at 2000 rpm for 15 minutes. To obtain information about the functional state of the body, biochemical tests were selected that reliably reflect the state of metabolic processes as carbohydrate, lipid protein: general protein, albumins, alanineaminotransferase, aspartaminotransferase, alkaline phosphatase, α-amylase, glucose, urea, creatinine.Results. Animals in the process of growth and development showed an increase in a number of indicators: urea, creatinine, total protein, albumin and glucose. ALT and AST activity, on the contrary, decreased with age, reaching minimum values 2 in 5-month-old animals. Alkaline phosphatase concentration also decreased with age. The level of α amylase during the growth and development of the animal did not undergo significant changes. Indicators of protein, carbohydrate metabolism, as well as renal markers and enzymes in fox-bearing hybrids are characterized by sex differences. Thus, the biochemical composition of the blood during the growth and development of the animal undergoes a number of changes. The collection and systematization of data will allow the calculation of the reference boundaries of the main blood parameters of lisopesse hybrids to assess the biochemical status of the beast by sex and age.
{"title":"Physical and biochemical profile of fox-gestational hybrids by sex and age","authors":"U. Berezina, M. Kochurnikova, O. Bespyatykh, I. Domskiy","doi":"10.31043/2410-2733-2022-3-92-98","DOIUrl":"https://doi.org/10.31043/2410-2733-2022-3-92-98","url":null,"abstract":"Purpose: The study of biochemical indicators of blood of females and males of fox hybrids with age.Materials and methods. Research was carried out on females and males of fox hybrids. The age dynamics of changes in the biochemical parameters of the blood was evaluated with a comparative analysis of 2 age groups: females and males of fox hybrids 2 and 5 months (n = 30). The animals were fed once a day (in the morning) with meat mixtures in accordance with age and physiological condition, contained under the same conditions. Blood from the animals was taken into a special test tube with an activator of a clot of lateral subcutaneous vein of the lower leg until morning feeding. They received serum from it by centrifugation at 2000 rpm for 15 minutes. To obtain information about the functional state of the body, biochemical tests were selected that reliably reflect the state of metabolic processes as carbohydrate, lipid protein: general protein, albumins, alanineaminotransferase, aspartaminotransferase, alkaline phosphatase, α-amylase, glucose, urea, creatinine.Results. Animals in the process of growth and development showed an increase in a number of indicators: urea, creatinine, total protein, albumin and glucose. ALT and AST activity, on the contrary, decreased with age, reaching minimum values 2 in 5-month-old animals. Alkaline phosphatase concentration also decreased with age. The level of α amylase during the growth and development of the animal did not undergo significant changes. Indicators of protein, carbohydrate metabolism, as well as renal markers and enzymes in fox-bearing hybrids are characterized by sex differences. Thus, the biochemical composition of the blood during the growth and development of the animal undergoes a number of changes. The collection and systematization of data will allow the calculation of the reference boundaries of the main blood parameters of lisopesse hybrids to assess the biochemical status of the beast by sex and age.","PeriodicalId":346303,"journal":{"name":"Genetics and breeding of animals","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125545315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-12DOI: 10.31043/2410-2733-2022-3-42-48
A. Kurilova, L. Karpenko, V. Maksimov, A. Bakhta
Purpose: to study the effects of a selenic -containing drug on the concentration of selenium in blood serum, organs and tissues of lambs of the Romanov breed.Materials and methods. In the course of the study, 120 novels of the Romanov breed and 80 lambs obtained from the data of disemeters were used. The formation of control and experimental groups was carried out taking into account the principle of pair analysis. In experiments on assessing the effect of the drug, clinically healthy animals were used on the body. During the study, four groups of lambs were formed at the age of 30 days. The mothers of the first group during the period of suyagnity received the drug “e-sel” at the rate of 1 ml per 50 kg of live weight; The lambs of the first experimental group introduced the drug “E-sel” at the rate of 0.2 ml per 10 kg of live weight according to the instructions. The second group was a control - a physiological solution was administered in the same quantity as the drug with experimental animals; Feeding was carried out by the main diet. The studies were carried out in the period before the chopping (4 months of age) was carried out. Blood fence was carried out from the jugular vein. The concentration of selenium in blood serum lambs was determined by fluorimetrically with 2.3-diaminonaftalin. After the chopping, the lambs were slaughtered. The concentration of selenium in the organs and tissues of the lamb was examined by atomic-adsorption spectrometry.Results. It was established that the concentration of selenium in the lambs of the first experimental group increased, starting from the 60s from the moment of birth. At the same time, in the lambs of the first experimental group, the concentration increased 2.84 times by the 60th knocks compared to control, 4.08 times compared to control by the 90s from the moment of birth and 8.5 times by 120 -It day from the moment of birth. The concentration of selenium in the kidneys, liver and muscles (the broadest back muscle) of the first experimental group significantly exceeded the values of control. The maximum concentration was noted in the kidneys. No reliable changes were noted between the 1st and 2nd experimental groups in the concentrations of selenium in the kidneys and liver. The concentration of selenium in the liver of the experimental group was 2.94 times higher in comparison with control. In the kidneys, the concentration of selenium was 2.26 times higher for the experimental group in comparison with control. It is noted that an increase in the concentration of selenium in the muscles exceeded control indicators of 4.59, respectively.
{"title":"Study of the effect of a seven -containing drug on the concentration of selenium in the organs and tissues of the lambs of the Romanov breed in the conditions of industrial complexes North-Western region","authors":"A. Kurilova, L. Karpenko, V. Maksimov, A. Bakhta","doi":"10.31043/2410-2733-2022-3-42-48","DOIUrl":"https://doi.org/10.31043/2410-2733-2022-3-42-48","url":null,"abstract":"Purpose: to study the effects of a selenic -containing drug on the concentration of selenium in blood serum, organs and tissues of lambs of the Romanov breed.Materials and methods. In the course of the study, 120 novels of the Romanov breed and 80 lambs obtained from the data of disemeters were used. The formation of control and experimental groups was carried out taking into account the principle of pair analysis. In experiments on assessing the effect of the drug, clinically healthy animals were used on the body. During the study, four groups of lambs were formed at the age of 30 days. The mothers of the first group during the period of suyagnity received the drug “e-sel” at the rate of 1 ml per 50 kg of live weight; The lambs of the first experimental group introduced the drug “E-sel” at the rate of 0.2 ml per 10 kg of live weight according to the instructions. The second group was a control - a physiological solution was administered in the same quantity as the drug with experimental animals; Feeding was carried out by the main diet. The studies were carried out in the period before the chopping (4 months of age) was carried out. Blood fence was carried out from the jugular vein. The concentration of selenium in blood serum lambs was determined by fluorimetrically with 2.3-diaminonaftalin. After the chopping, the lambs were slaughtered. The concentration of selenium in the organs and tissues of the lamb was examined by atomic-adsorption spectrometry.Results. It was established that the concentration of selenium in the lambs of the first experimental group increased, starting from the 60s from the moment of birth. At the same time, in the lambs of the first experimental group, the concentration increased 2.84 times by the 60th knocks compared to control, 4.08 times compared to control by the 90s from the moment of birth and 8.5 times by 120 -It day from the moment of birth. The concentration of selenium in the kidneys, liver and muscles (the broadest back muscle) of the first experimental group significantly exceeded the values of control. The maximum concentration was noted in the kidneys. No reliable changes were noted between the 1st and 2nd experimental groups in the concentrations of selenium in the kidneys and liver. The concentration of selenium in the liver of the experimental group was 2.94 times higher in comparison with control. In the kidneys, the concentration of selenium was 2.26 times higher for the experimental group in comparison with control. It is noted that an increase in the concentration of selenium in the muscles exceeded control indicators of 4.59, respectively.","PeriodicalId":346303,"journal":{"name":"Genetics and breeding of animals","volume":"44 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114758207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-12DOI: 10.31043/2410-2733-2022-3-69-85
A. Supilnikov, O. Pavlova, D. Liu, E. Dorozhkina, P. Smelova
The present work is devoted to the study of the course of the wound process in animals as well as possible factors influencing it. The topicality of the research in this topic consists in the fact that the experimental results help to increase the survival rate among the animals which have undergone the operation, to accelerate healing, to reduce the organism rehabilitation time, to avoid the bacterial contamination of wounds, purulent diseases and re-infection, to reduce the trauma level in animals, promote the introduction of new efficient pharmacological methods of prevention and suppression of surgical infections, application of laser and ultrasound equipment, discover and use new medicines. The aim of the study. The aim of this work is to conduct a general literature review on the methods of wound management in animals. This review focuses on papers from 2012 to 2022, citing various factors that influence the wound process in order to outline the need for wound management. To make it more manageable and to speed up wound healing in animals, as well as to point out the need for additional wound management models. Conclusion. The use of a complex graft and matrix based on dextran phosphate gel, stem cells and miliacyl has been shown by biological factors to promote healing. A positive alkaline environment has been shown to promote reepithelisation, as it has no adverse effect, in contrast to a strongly acidic environment. In addition, gamma radiation treatment was found to accelerate healing. From this we can conclude that the literature review conducted on this topic indicates the relevance of the area under consideration, and further developments and more in-depth studies are required, as wound processes are a scientific problem that needs to be solved.
{"title":"Review of methods of impact on the wound process animals (literary review)","authors":"A. Supilnikov, O. Pavlova, D. Liu, E. Dorozhkina, P. Smelova","doi":"10.31043/2410-2733-2022-3-69-85","DOIUrl":"https://doi.org/10.31043/2410-2733-2022-3-69-85","url":null,"abstract":"The present work is devoted to the study of the course of the wound process in animals as well as possible factors influencing it. The topicality of the research in this topic consists in the fact that the experimental results help to increase the survival rate among the animals which have undergone the operation, to accelerate healing, to reduce the organism rehabilitation time, to avoid the bacterial contamination of wounds, purulent diseases and re-infection, to reduce the trauma level in animals, promote the introduction of new efficient pharmacological methods of prevention and suppression of surgical infections, application of laser and ultrasound equipment, discover and use new medicines. The aim of the study. The aim of this work is to conduct a general literature review on the methods of wound management in animals. This review focuses on papers from 2012 to 2022, citing various factors that influence the wound process in order to outline the need for wound management. To make it more manageable and to speed up wound healing in animals, as well as to point out the need for additional wound management models. Conclusion. The use of a complex graft and matrix based on dextran phosphate gel, stem cells and miliacyl has been shown by biological factors to promote healing. A positive alkaline environment has been shown to promote reepithelisation, as it has no adverse effect, in contrast to a strongly acidic environment. In addition, gamma radiation treatment was found to accelerate healing. From this we can conclude that the literature review conducted on this topic indicates the relevance of the area under consideration, and further developments and more in-depth studies are required, as wound processes are a scientific problem that needs to be solved.","PeriodicalId":346303,"journal":{"name":"Genetics and breeding of animals","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130674757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-12DOI: 10.31043/2410-2733-2022-3-105-110
N. Pleshanov, A. Kurochkin, A. Nakidkina
Purpose: to study the influence of the level of active forms of oxygen in native spermatozoa of roosters on the qualitative indicators of freshly exposed and deconed individual ejaculators (mobility, damage to the cell membranes of sperm) and determining the permissible level of AFC generation to improve the cryoponement protocol.Materials and methods. The object of the study was the roosters of the Rodi-Aland Red (n = 20) breed at the age of 44 weeks of life. All males were kept in individual cells with the “Genetic Collection of Rare and Disappearing Course breeds” of VNIIGRZH systems adopted by the BRK for feeding, posting and light regime. Sperm was received in penicillin bottles with a volume of 10 ml, using the abdominal massage method. They measured the volume of each individual ejaculate, assessed the mobility of sperm, concentration. Cryoconservational was carried out in granules. Thawing of granules was carried out at T 60 ° C in a slit tie. The damage to the plasma membranes of sperm in the native and deconed seed was evaluated using the Suppitial Bluma coloring method. Spermatozoa with damaged membranes was painted red, intact cells remained white (colorless). Each drug estimates at least 200 cells. To determine the levels of AFC generation in spermatozoa of roosters, a method based on luminol-proroxidate hemilyuminescence was used, which was measured on a chemilyuminometer Lum-1200. The time of each measurement was 3 hours, based on the hemiluminiscence of the active form of oxygen (given the growth of the indicator, peak and decline). Cell concentration (7x106 classes/ml) was selected experimentally for measurements, according to the results of a series of preliminary experiments.Results. As a result of the study, data based on the method of luminol-proroxidate chemilyuminescence for the permissible level of AFC in native sperm of roosters were first obtained. The range of active forms of oxygen (from 75 to 249 volts*sec) has been established, in which cells do not receive significant damage to membrane structures during cryoponservation. In case of exceeding the threshold of 250 volts*S, the number of cells with damaged membranes increases sharply from 17.19% to 62.87%. The data obtained allow the assessment and selection of roosters on the quality of their sperm for the purposes of cryoponservation and the formation of cryobans of reproductive cells.
{"title":"Relationship between the level of reactive oxygen species in native rooster spermatozoa and the quality indicators of fresh and frozen-thawed sperm","authors":"N. Pleshanov, A. Kurochkin, A. Nakidkina","doi":"10.31043/2410-2733-2022-3-105-110","DOIUrl":"https://doi.org/10.31043/2410-2733-2022-3-105-110","url":null,"abstract":"Purpose: to study the influence of the level of active forms of oxygen in native spermatozoa of roosters on the qualitative indicators of freshly exposed and deconed individual ejaculators (mobility, damage to the cell membranes of sperm) and determining the permissible level of AFC generation to improve the cryoponement protocol.Materials and methods. The object of the study was the roosters of the Rodi-Aland Red (n = 20) breed at the age of 44 weeks of life. All males were kept in individual cells with the “Genetic Collection of Rare and Disappearing Course breeds” of VNIIGRZH systems adopted by the BRK for feeding, posting and light regime. Sperm was received in penicillin bottles with a volume of 10 ml, using the abdominal massage method. They measured the volume of each individual ejaculate, assessed the mobility of sperm, concentration. Cryoconservational was carried out in granules. Thawing of granules was carried out at T 60 ° C in a slit tie. The damage to the plasma membranes of sperm in the native and deconed seed was evaluated using the Suppitial Bluma coloring method. Spermatozoa with damaged membranes was painted red, intact cells remained white (colorless). Each drug estimates at least 200 cells. To determine the levels of AFC generation in spermatozoa of roosters, a method based on luminol-proroxidate hemilyuminescence was used, which was measured on a chemilyuminometer Lum-1200. The time of each measurement was 3 hours, based on the hemiluminiscence of the active form of oxygen (given the growth of the indicator, peak and decline). Cell concentration (7x106 classes/ml) was selected experimentally for measurements, according to the results of a series of preliminary experiments.Results. As a result of the study, data based on the method of luminol-proroxidate chemilyuminescence for the permissible level of AFC in native sperm of roosters were first obtained. The range of active forms of oxygen (from 75 to 249 volts*sec) has been established, in which cells do not receive significant damage to membrane structures during cryoponservation. In case of exceeding the threshold of 250 volts*S, the number of cells with damaged membranes increases sharply from 17.19% to 62.87%. The data obtained allow the assessment and selection of roosters on the quality of their sperm for the purposes of cryoponservation and the formation of cryobans of reproductive cells.","PeriodicalId":346303,"journal":{"name":"Genetics and breeding of animals","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130073854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-12DOI: 10.31043/2410-2733-2022-3-111-118
A. Petrova, E. Vasileva
Purpose: the goal is to study the abundance, distribution area and genetic similarity of regional populations of the Ayrshire breed of the Russian Federation for the possibility of forming a reference population and conducting a genomic assessment using modern methods for small populations of cattle.Materials and methods. The material was articles and presentations of domestic and foreign researchers, statistical data on the breeding and bovine livestock of the Ayrshire breed of dairy cattle in yearbooks, bulletins and catalogs for evaluating bulls of enterprises in Russia. Studied and analyzed electronic resources.Results. To introduce genomic selection (GS) for Ayrshire cattle of Russia, it is necessary to form a reference population, which will be used to link molecular markers (SNPs) with the breeding value of animals evaluated using the BLUP method. At the same time, with an increase in the accuracy of the assessment of animals and the accumulation of livestock, the reliability of the genomic assessment increases. Accounting for the productive traits of the descendants and daughters of bulls is necessary to replenish and update the reference population in order to obtain a more accurate genomic assessment in the future. With the introduction of GS, the genetic progress of the population will accelerate, the generation interval and economic costs for the producer will decrease. The accuracy of genomic prediction increases significantly and will exceed the prediction for parental individuals.Conclusion. As a result of the analysis, it was found that in the coming years it is possible to form a reference population for the genomic assessment of Ayrshire cattle. The reference population will allow as soon as possible to start the process of introducing GS to breed highly productive domestic animals, since today the Ayrshire cattle of Russia cannot compete not only at the world level with the leaders of animal husbandry, but also within the country with imported genetics.
{"title":"Possibility of creating a reference population of Ayrshire cattle","authors":"A. Petrova, E. Vasileva","doi":"10.31043/2410-2733-2022-3-111-118","DOIUrl":"https://doi.org/10.31043/2410-2733-2022-3-111-118","url":null,"abstract":"Purpose: the goal is to study the abundance, distribution area and genetic similarity of regional populations of the Ayrshire breed of the Russian Federation for the possibility of forming a reference population and conducting a genomic assessment using modern methods for small populations of cattle.Materials and methods. The material was articles and presentations of domestic and foreign researchers, statistical data on the breeding and bovine livestock of the Ayrshire breed of dairy cattle in yearbooks, bulletins and catalogs for evaluating bulls of enterprises in Russia. Studied and analyzed electronic resources.Results. To introduce genomic selection (GS) for Ayrshire cattle of Russia, it is necessary to form a reference population, which will be used to link molecular markers (SNPs) with the breeding value of animals evaluated using the BLUP method. At the same time, with an increase in the accuracy of the assessment of animals and the accumulation of livestock, the reliability of the genomic assessment increases. Accounting for the productive traits of the descendants and daughters of bulls is necessary to replenish and update the reference population in order to obtain a more accurate genomic assessment in the future. With the introduction of GS, the genetic progress of the population will accelerate, the generation interval and economic costs for the producer will decrease. The accuracy of genomic prediction increases significantly and will exceed the prediction for parental individuals.Conclusion. As a result of the analysis, it was found that in the coming years it is possible to form a reference population for the genomic assessment of Ayrshire cattle. The reference population will allow as soon as possible to start the process of introducing GS to breed highly productive domestic animals, since today the Ayrshire cattle of Russia cannot compete not only at the world level with the leaders of animal husbandry, but also within the country with imported genetics.","PeriodicalId":346303,"journal":{"name":"Genetics and breeding of animals","volume":"47 24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124716398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-12DOI: 10.31043/2410-2733-2022-3-49-54
L. Korelskaya, L. Sosnina, S. Kolomiets
Purpose: within the framework of the research work, studies were carried out on the biochemical composition of the blood serum of highly productive cows, different periods of lactation and during the dry period, with tethered and loose housing.Materials and methods. The object of the study were cows of black-and-white Holsteinized breed with a productivity of more than 8500 kg on the farm. Blood sampling for biotesting was taken from 108 cows before morning feeding. Animals of the experimental groups were formed according to different periods of lactation and during the dry period with different methods of keeping. The study was conducted on the basis of the laboratory of biochemistry and physiology of animals of the SZNIIMLPKh named after A. S. Emelyanov.Results. In the period from 1-100 days of lactation, there is an increase in calcium from physiological norms by 9.6%, calcium-phosphorus ratio by 32.6 % and phosphorus deficiency by 6.7 %. Fluctuations in the level of calcium in the blood serum from 7.89-12 mg%, phosphorus from 3.02-3.75 mg%. During the peak of lactation, a decrease in the amount of calcium by 2.7 % is noticeable, as well as a calcium-phosphorus ratio by 1.2% an increase in phosphorus by 4.6 %. Within the group, calcium and phosphorus values ranged from 7.13-13.74 mg%, 2.86-4.48 mg%, respectively. During the period of attenuation of lactation, an increase in the content of calcium by 5.3 %, calcium-phosphorus ratio by 10.8%, a slight decrease in phosphorus by 1.3 % is observed. Calcium values range from 8.23-12.32 mg%, phosphorus from 3.02-4.69 mg%. In the dry period, there is a deviation from the reference values of calcium, phosphorus, calcium-phosphorus ratio by 14.4; 6.7 and 8.4 %, re spectively. There is a difference in the level of calcium within the group from 5.82-10.64 mg%, phosphorus from 2.94-4.05 mg%. The level of calcium, calcium-phosphorus ratio in the blood serum of the examined animals in all periods of lactation on loose content are below the norm by 2.3-11.8 % and 6.7-13.6 %, respectively. In the blood of animals of all groups, calcium values range from 4.36-13.45 mg%, phosphorus from 3.06-5.24 mg%.
{"title":"Live weight as an indicator of morphobiochemical blood composition of heifers under intensive breeding technology","authors":"L. Korelskaya, L. Sosnina, S. Kolomiets","doi":"10.31043/2410-2733-2022-3-49-54","DOIUrl":"https://doi.org/10.31043/2410-2733-2022-3-49-54","url":null,"abstract":"Purpose: within the framework of the research work, studies were carried out on the biochemical composition of the blood serum of highly productive cows, different periods of lactation and during the dry period, with tethered and loose housing.Materials and methods. The object of the study were cows of black-and-white Holsteinized breed with a productivity of more than 8500 kg on the farm. Blood sampling for biotesting was taken from 108 cows before morning feeding. Animals of the experimental groups were formed according to different periods of lactation and during the dry period with different methods of keeping. The study was conducted on the basis of the laboratory of biochemistry and physiology of animals of the SZNIIMLPKh named after A. S. Emelyanov.Results. In the period from 1-100 days of lactation, there is an increase in calcium from physiological norms by 9.6%, calcium-phosphorus ratio by 32.6 % and phosphorus deficiency by 6.7 %. Fluctuations in the level of calcium in the blood serum from 7.89-12 mg%, phosphorus from 3.02-3.75 mg%. During the peak of lactation, a decrease in the amount of calcium by 2.7 % is noticeable, as well as a calcium-phosphorus ratio by 1.2% an increase in phosphorus by 4.6 %. Within the group, calcium and phosphorus values ranged from 7.13-13.74 mg%, 2.86-4.48 mg%, respectively. During the period of attenuation of lactation, an increase in the content of calcium by 5.3 %, calcium-phosphorus ratio by 10.8%, a slight decrease in phosphorus by 1.3 % is observed. Calcium values range from 8.23-12.32 mg%, phosphorus from 3.02-4.69 mg%. In the dry period, there is a deviation from the reference values of calcium, phosphorus, calcium-phosphorus ratio by 14.4; 6.7 and 8.4 %, re spectively. There is a difference in the level of calcium within the group from 5.82-10.64 mg%, phosphorus from 2.94-4.05 mg%. The level of calcium, calcium-phosphorus ratio in the blood serum of the examined animals in all periods of lactation on loose content are below the norm by 2.3-11.8 % and 6.7-13.6 %, respectively. In the blood of animals of all groups, calcium values range from 4.36-13.45 mg%, phosphorus from 3.06-5.24 mg%.","PeriodicalId":346303,"journal":{"name":"Genetics and breeding of animals","volume":"34 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134015827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-12DOI: 10.31043/2410-2733-2022-3-119-123
O. Yudina, K. Ragimova, A. Anokhin
Purpose: comparison of working capacity of service dogs of different breeds depending on their use at different times of the day.Materials and methods. The studies were carried out on service dogs (n=41) of various breeds: German (18 heads) and Belgian (Malinois) (7 heads) shepherd dogs, Labrador (13 heads), Spaniel (3 heads), used in the following areas: search for explosives and explosive devices, the search for narcotic drugs and substances and the general search profile. The age of the dogs is from 3 to 9 years. Feeding of the entire livestock was carried out with dry complete feed. The effectiveness of the use of service dogs depending on the breed, sex and time of day has been studied. The day was divided into morning - from 6.00 to 12.00, afternoon - from 12.00 to 18.00, evening - from 18.00 to 24.00 and night - from 24.00 to 6.00. Data on the working qualities of dogs in each of the areas are taken from the reporting documents of the "Acts on the use of service dogs" for 2019.Results. It was found that German Shepherds worked better in the search for explosives in the morning, afternoon and evening - from 0.6 to 0.83% of successful trips, Labradors stood out at night - 1.77%. In the other direction - the search for narcotic substances in the morning, the best result was shown by spaniels - 33.3% of successful trips, in the daytime and at night - by Belgian shepherds - 38.5% and 33.35%, respectively. In the evening – German Shepherds - 44.4%. Comparison of general detective dogs showed no significant differences between breeds. It should be noted that regardless of the breed and direction of application, bitches worked better at night.
{"title":"The influence of the time of day on the working qualities of service dogs","authors":"O. Yudina, K. Ragimova, A. Anokhin","doi":"10.31043/2410-2733-2022-3-119-123","DOIUrl":"https://doi.org/10.31043/2410-2733-2022-3-119-123","url":null,"abstract":"Purpose: comparison of working capacity of service dogs of different breeds depending on their use at different times of the day.Materials and methods. The studies were carried out on service dogs (n=41) of various breeds: German (18 heads) and Belgian (Malinois) (7 heads) shepherd dogs, Labrador (13 heads), Spaniel (3 heads), used in the following areas: search for explosives and explosive devices, the search for narcotic drugs and substances and the general search profile. The age of the dogs is from 3 to 9 years. Feeding of the entire livestock was carried out with dry complete feed. The effectiveness of the use of service dogs depending on the breed, sex and time of day has been studied. The day was divided into morning - from 6.00 to 12.00, afternoon - from 12.00 to 18.00, evening - from 18.00 to 24.00 and night - from 24.00 to 6.00. Data on the working qualities of dogs in each of the areas are taken from the reporting documents of the \"Acts on the use of service dogs\" for 2019.Results. It was found that German Shepherds worked better in the search for explosives in the morning, afternoon and evening - from 0.6 to 0.83% of successful trips, Labradors stood out at night - 1.77%. In the other direction - the search for narcotic substances in the morning, the best result was shown by spaniels - 33.3% of successful trips, in the daytime and at night - by Belgian shepherds - 38.5% and 33.35%, respectively. In the evening – German Shepherds - 44.4%. Comparison of general detective dogs showed no significant differences between breeds. It should be noted that regardless of the breed and direction of application, bitches worked better at night.","PeriodicalId":346303,"journal":{"name":"Genetics and breeding of animals","volume":"9 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129267029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-12DOI: 10.31043/2410-2733-2022-3-86-91
A. Kurilova, L. Karpenko, V. Maksimov, A. Bakhta
Purpose: to study the concentration of selenium in milk and the impact of the introduction of selenium preparations on the concentration of a trace element in milk at various periods of lactation at the Romanovskoye sheep in the conditions of industrial complexes of the North-Western region.Materials and methods. 80 disemetals of the Romanov breed aged 1 year are involved. Used clinically healthy animals. The diet was compiled taking into account generally accepted recommendations, depending on age and physiological condition. The content of selenium in plants that were used in the diet of animals was determined by fluorimetrically with 2.3-diaminonaphthalin. At the first stage of the study, a group of disemeters was formed after a backed, milk was received by selecting a control milking after 5, 30, 60, 90, 120th day after the start of lactation. The concentration of selenium in milk was studied by atomic-adsorption spectrometry. At the second stage of the study, two groups of disemeters of the Romanov breed aged 1 year were formed. Pregnancy was confirmed by the visual and evaluation method for the lack of hunting in sheep 20-30 days after the derive period, by the lamb-industry and 2.5 months after the shed period by palpation. During the period of pregnancy, the animals of the first experimental group administered intramuscularly drug “E-sel” at the rate of 1 ml per 50 kg of live weight according to the instructions of the drug; The second group, control, introduced a physiological solution in the same concentration as the drug experimental animals. In animals of the experimental and control groups, a study of the concentration of selenium in milk was carried out by selecting a test milking samples after 5, 30, 60, 90, 120th day after lactation.Results. The concentration of selenium was reduced in various periods of lactation, which is more likely to be associated with a decrease in the total amount of milk produced and the end of the lactation period. In the continuation of the lactation period, the concentration of selenium was lower than reference values. The concentration of selenium in milk was reliably higher in the disetals, which during pregnancy were administered the drug Selena. However, by the second month of lactation, the concentration of the trace element in milk decreased by 47.72 % in relation to the initial period of lactation. It was noted that with the course of lactation and cancellation of the cottage of drugs there is a significant decrease in the concentration of selenium in the milk of the disemets. On the 120th day, lactation concentration of selenium decreased by 5.9 times compared to 5 days of lactation and 4 times for the control group. Thus, it follows that the concentration of selenium in the milk of the disemeters becomes low on the 30th day, therefore, the milk of mothers cannot be a full-fledged source of selenium for growing lambs, since the first, second week after birth was fed exclusively milk, starting fr
{"title":"The content of selenium in the milk of the sheep of the Romanovsky breed in different periods of lactation","authors":"A. Kurilova, L. Karpenko, V. Maksimov, A. Bakhta","doi":"10.31043/2410-2733-2022-3-86-91","DOIUrl":"https://doi.org/10.31043/2410-2733-2022-3-86-91","url":null,"abstract":"Purpose: to study the concentration of selenium in milk and the impact of the introduction of selenium preparations on the concentration of a trace element in milk at various periods of lactation at the Romanovskoye sheep in the conditions of industrial complexes of the North-Western region.Materials and methods. 80 disemetals of the Romanov breed aged 1 year are involved. Used clinically healthy animals. The diet was compiled taking into account generally accepted recommendations, depending on age and physiological condition. The content of selenium in plants that were used in the diet of animals was determined by fluorimetrically with 2.3-diaminonaphthalin. At the first stage of the study, a group of disemeters was formed after a backed, milk was received by selecting a control milking after 5, 30, 60, 90, 120th day after the start of lactation. The concentration of selenium in milk was studied by atomic-adsorption spectrometry. At the second stage of the study, two groups of disemeters of the Romanov breed aged 1 year were formed. Pregnancy was confirmed by the visual and evaluation method for the lack of hunting in sheep 20-30 days after the derive period, by the lamb-industry and 2.5 months after the shed period by palpation. During the period of pregnancy, the animals of the first experimental group administered intramuscularly drug “E-sel” at the rate of 1 ml per 50 kg of live weight according to the instructions of the drug; The second group, control, introduced a physiological solution in the same concentration as the drug experimental animals. In animals of the experimental and control groups, a study of the concentration of selenium in milk was carried out by selecting a test milking samples after 5, 30, 60, 90, 120th day after lactation.Results. The concentration of selenium was reduced in various periods of lactation, which is more likely to be associated with a decrease in the total amount of milk produced and the end of the lactation period. In the continuation of the lactation period, the concentration of selenium was lower than reference values. The concentration of selenium in milk was reliably higher in the disetals, which during pregnancy were administered the drug Selena. However, by the second month of lactation, the concentration of the trace element in milk decreased by 47.72 % in relation to the initial period of lactation. It was noted that with the course of lactation and cancellation of the cottage of drugs there is a significant decrease in the concentration of selenium in the milk of the disemets. On the 120th day, lactation concentration of selenium decreased by 5.9 times compared to 5 days of lactation and 4 times for the control group. Thus, it follows that the concentration of selenium in the milk of the disemeters becomes low on the 30th day, therefore, the milk of mothers cannot be a full-fledged source of selenium for growing lambs, since the first, second week after birth was fed exclusively milk, starting fr","PeriodicalId":346303,"journal":{"name":"Genetics and breeding of animals","volume":"6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121357775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-12DOI: 10.31043/2410-2733-2022-3-124-128
T. Ippolitova, A. Veberg, A. Lukashin
Purpose: To assess the degree of emotional stress in clinically healthy dogs of the Husky breed with a different method of fixation, according to the indicators of variational heart rate measurement, in accordance with the indices of R.M. Baevsky. Data indicating a direct dependence on the method of fixation, as well as the method of registration of the electrocardiogram, on the autonomic regulation of the heart rhythm were obtained. Methods for recording an electrocardiogram with the least pronounced effect on humoral and vegetative tone are allocated, the degree of predominance of the interaction of the sympathetic and parasympathetic nervous system in the regulation of the cardiac cycle in dogs is determined.Materials and methods. To conduct the study, a group of dogs was formed, husky breeds that included clinically healthy 11 dogs with a live weight of 19 to 25 kg. Age restrictions ranged from 2 to 7 years. The electrocardiogram was recorded using the Conan hardware and software complex.Outcomes. Data indicating a direct dependence on the method of fixation, as well as the method of registration of the electrocardiogram, on the autonomic regulation of the heart rhythm were obtained. Methods for recording an electrocardiogram with the least pronounced effect on humoral and vegetative tone are allocated, the degree of predominance of the interaction of the sympathetic and parasympathetic nervous system in the regulation of the cardiac cycle in dogs is determined.Conclusion. In the course of the interpretation of the data obtained, it is possible to conclude: the use of electrocardiogram registration techniques using rigid fixation has a more pronounced effect on the vegetative tone, as well as the emotional state of the dogs under study. Taking into account this factor, it should be noted that despite the absence of indications characteristic of diseases in the studied group of animals, variational pulsometry according to the indices of Baevsky R. M. is applicable not only to extract deeper, more valuable prognostic information about the nature of possible changes in the myocardium and coronary vessels under various micro and macro effects on the body, but also to obtain information about the balance and stability of self-regulation processes.
目的:采用不同固定方法，根据变分心率测量指标，按照R.M. Baevsky指标，评价临床健康哈士奇犬的情绪应激程度。数据表明直接依赖于固定方法，以及心电图的登记方法，对心律的自主调节。分配了对体液和植物性张力影响最小的心电图记录方法，确定了交感和副交感神经系统在狗心脏周期调节中的相互作用的优势程度。材料和方法。为了进行这项研究，研究人员组建了一组狗，哈士奇品种，其中包括11只临床健康的狗，活重为19至25公斤。年龄限制从2岁到7岁不等。使用Conan硬件和软件复合体记录心电图。数据表明直接依赖于固定方法，以及心电图的登记方法，对心律的自主调节。分配了对体液和植物性张力影响最小的心电图记录方法，确定了交感和副交感神经系统在狗心脏周期调节中的相互作用的优势程度。在对获得的数据进行解释的过程中，可以得出这样的结论:采用刚性固定的心电图登记技术对所研究犬的植物性张力和情绪状态有更明显的影响。考虑到这一因素，应该注意的是，尽管在研究的动物群体中没有疾病的特征性适应症，但根据Baevsky R. M.的指标进行的变分脉搏测量不仅适用于提取更深入、更有价值的预后信息，了解在各种微观和宏观作用下心肌和冠状血管可能变化的性质，也是为了获得关于自我调节过程的平衡和稳定的信息。
{"title":"The effect of fixation of dogs on the autonomic nervous system, when registering an electrocardiogram","authors":"T. Ippolitova, A. Veberg, A. Lukashin","doi":"10.31043/2410-2733-2022-3-124-128","DOIUrl":"https://doi.org/10.31043/2410-2733-2022-3-124-128","url":null,"abstract":"Purpose: To assess the degree of emotional stress in clinically healthy dogs of the Husky breed with a different method of fixation, according to the indicators of variational heart rate measurement, in accordance with the indices of R.M. Baevsky. Data indicating a direct dependence on the method of fixation, as well as the method of registration of the electrocardiogram, on the autonomic regulation of the heart rhythm were obtained. Methods for recording an electrocardiogram with the least pronounced effect on humoral and vegetative tone are allocated, the degree of predominance of the interaction of the sympathetic and parasympathetic nervous system in the regulation of the cardiac cycle in dogs is determined.Materials and methods. To conduct the study, a group of dogs was formed, husky breeds that included clinically healthy 11 dogs with a live weight of 19 to 25 kg. Age restrictions ranged from 2 to 7 years. The electrocardiogram was recorded using the Conan hardware and software complex.Outcomes. Data indicating a direct dependence on the method of fixation, as well as the method of registration of the electrocardiogram, on the autonomic regulation of the heart rhythm were obtained. Methods for recording an electrocardiogram with the least pronounced effect on humoral and vegetative tone are allocated, the degree of predominance of the interaction of the sympathetic and parasympathetic nervous system in the regulation of the cardiac cycle in dogs is determined.Conclusion. In the course of the interpretation of the data obtained, it is possible to conclude: the use of electrocardiogram registration techniques using rigid fixation has a more pronounced effect on the vegetative tone, as well as the emotional state of the dogs under study. Taking into account this factor, it should be noted that despite the absence of indications characteristic of diseases in the studied group of animals, variational pulsometry according to the indices of Baevsky R. M. is applicable not only to extract deeper, more valuable prognostic information about the nature of possible changes in the myocardium and coronary vessels under various micro and macro effects on the body, but also to obtain information about the balance and stability of self-regulation processes.","PeriodicalId":346303,"journal":{"name":"Genetics and breeding of animals","volume":"214 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132391376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-12DOI: 10.31043/2410-2733-2022-3-99-104
M. Atroshchenko, M. Engalycheva, A. Shitikova
The indicators of the biochemical analysis of the blood of horses reflect the state of metabolism of the animal and at the same time are potential predictors of the qualitative characteristics of spermatozoa, the freezing of which is one of the most important ways to preserve and further use the genetic material. The aim of this study was to identify correlations between the level of such acute phase blood proteins, as C-reactive protein and fibrinogen, and the qualitative and quantitative characteristics of the native and thawed sperm of stallions. The object of the study was blood and sperm samples from 87 stallions of various breeds, the average age of which was 10.5±0.5 years. To assess the protein status of the blood, in addition to the aforementioned acute phase proteins, the total protein content, the levels of albumin, globulins, and the albumin / globulin ratio were determined. Stallion sperm samples were assessed according to the following indicators: ejaculate volume, concentration, the total number of spermatozoa in the ejaculate, progressive motility and survival at +4°C. When analyzing the correlations between the studied parameters, a statistically significant negative inverse relationship was revealed between the level of fibrinogen in the blood serum and such parameters as the content of progressively motile sperm in native and thawed cryopreserved sperm (r=-0.22, p<0.05 and r=-0.29, p<0.01, respectively), as well as sperm survival in native and thawed sperm (r=-0.48, p<0.001 and r=-0.22, p<0.05, respectively).There was no statistically significant relationship between the level of C-reactive protein and the studied characteristics of the spermogram. The revealed negative correlation between the level of fibrinogen and the characteristics of the stallions’ sperm can be considered as one of the prognostic important indicators that must be taken into account when selecting stallions with the highest indicators of the quality of cryopreserved sperm.
{"title":"The level of acute phase blood proteins as a predictive criterion of the cryo resistance of stallion sperm","authors":"M. Atroshchenko, M. Engalycheva, A. Shitikova","doi":"10.31043/2410-2733-2022-3-99-104","DOIUrl":"https://doi.org/10.31043/2410-2733-2022-3-99-104","url":null,"abstract":"The indicators of the biochemical analysis of the blood of horses reflect the state of metabolism of the animal and at the same time are potential predictors of the qualitative characteristics of spermatozoa, the freezing of which is one of the most important ways to preserve and further use the genetic material. The aim of this study was to identify correlations between the level of such acute phase blood proteins, as C-reactive protein and fibrinogen, and the qualitative and quantitative characteristics of the native and thawed sperm of stallions. The object of the study was blood and sperm samples from 87 stallions of various breeds, the average age of which was 10.5±0.5 years. To assess the protein status of the blood, in addition to the aforementioned acute phase proteins, the total protein content, the levels of albumin, globulins, and the albumin / globulin ratio were determined. Stallion sperm samples were assessed according to the following indicators: ejaculate volume, concentration, the total number of spermatozoa in the ejaculate, progressive motility and survival at +4°C. When analyzing the correlations between the studied parameters, a statistically significant negative inverse relationship was revealed between the level of fibrinogen in the blood serum and such parameters as the content of progressively motile sperm in native and thawed cryopreserved sperm (r=-0.22, p<0.05 and r=-0.29, p<0.01, respectively), as well as sperm survival in native and thawed sperm (r=-0.48, p<0.001 and r=-0.22, p<0.05, respectively).There was no statistically significant relationship between the level of C-reactive protein and the studied characteristics of the spermogram. The revealed negative correlation between the level of fibrinogen and the characteristics of the stallions’ sperm can be considered as one of the prognostic important indicators that must be taken into account when selecting stallions with the highest indicators of the quality of cryopreserved sperm.","PeriodicalId":346303,"journal":{"name":"Genetics and breeding of animals","volume":"173 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115566789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：