Pub Date : 2023-05-18DOI: 10.31043/2410-2733-2023-1-74-83
A. Mikhaliuk, L. Tanana, T. Kuzmina
The paper presents the results of studying the associated effect of complex genotypes for the genes diacylglycerol O-acyl transferase 1 (DGAT1), somatotropin (GH), prolactin (PRL) and beta-lactoglobulin (BLG) on the milk productivity of Holstein dairy cattle of domestic selection. The object of research was cattle and biological material (ear pluck) from cows of Holstein dairy cattle of domestic selection, contained in the agricultural production cooperative named after I.P. Senko, Grodno region. DNA genotyping of animals for the genes of diacylglycerol O-acyl transferase 1 (DGAT1), somatotropin (GH), prolactin (PRL), and beta-lactoglobulin (BLG) was performed using the polymerase chain reaction (PCR) method and restriction fragment length polymorphism ( RFLP). The results of studies on the assessment of the associated effect of complex genotypes for the genes diacylglycerol O-acyl transferase 1 (DGAT1), somatotropin (GH), prolactin (PRL) and beta-lactoglobulin (BLG) on the milk productivity of Holstein cows of dairy cattle domestic selection showed that in terms of the mass fraction of fat and the amount of milk fat in milk, in most cases, the highest rates were in animals with the complex genotype DGAT1ККGHLLPRLAABLGAB.
{"title":"Association of the complex of polymorphic variants of the DGAT1, GH, PRL and BLG, genes with dairy productivity indicators of holstein dairy cattle cows of domestic selection","authors":"A. Mikhaliuk, L. Tanana, T. Kuzmina","doi":"10.31043/2410-2733-2023-1-74-83","DOIUrl":"https://doi.org/10.31043/2410-2733-2023-1-74-83","url":null,"abstract":"The paper presents the results of studying the associated effect of complex genotypes for the genes diacylglycerol O-acyl transferase 1 (DGAT1), somatotropin (GH), prolactin (PRL) and beta-lactoglobulin (BLG) on the milk productivity of Holstein dairy cattle of domestic selection. The object of research was cattle and biological material (ear pluck) from cows of Holstein dairy cattle of domestic selection, contained in the agricultural production cooperative named after I.P. Senko, Grodno region. DNA genotyping of animals for the genes of diacylglycerol O-acyl transferase 1 (DGAT1), somatotropin (GH), prolactin (PRL), and beta-lactoglobulin (BLG) was performed using the polymerase chain reaction (PCR) method and restriction fragment length polymorphism ( RFLP). The results of studies on the assessment of the associated effect of complex genotypes for the genes diacylglycerol O-acyl transferase 1 (DGAT1), somatotropin (GH), prolactin (PRL) and beta-lactoglobulin (BLG) on the milk productivity of Holstein cows of dairy cattle domestic selection showed that in terms of the mass fraction of fat and the amount of milk fat in milk, in most cases, the highest rates were in animals with the complex genotype DGAT1ККGHLLPRLAABLGAB.","PeriodicalId":346303,"journal":{"name":"Genetics and breeding of animals","volume":"10 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117222599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-18DOI: 10.31043/2410-2733-2023-1-96-103
A. Filatova, B. Tshivale, G. Nikitin, V. Avdeenko
Purpose: to establish the role of reproductive pathologies in high-yielding cows in reducing the sanitary quality of milk at dairy enterprises using various milking systems.Materials and methods. The studies were carried out in high-tech dairy enterprises using GEA Dairy ProQ and Ley Astronaut robotic milking systems with cow productivity of more than 11 tons of milk per lactation. 4659 lactating cows were under observation. The selection of samples of uterine secretions, samples from the udder teats, the surface of the anogenital area, mammary gland and milk for bacteriological studies was carried out sterile, then they were sown on nutrient media.Results. The contents of the uterus with pyometra in 80% are contaminated with microorganisms: S. dysgalactiae – 29.1%, S. aureus – 26.4%, E. coli – 24.2%, and S. agalactiae – 20.3%. In 12% of cows, the microflora was isolated in monoculture. In samples of milk obtained from sick cows with uterine pathology using Ley Astronaut robotic milking, it showed 1.72 times lower sanitary safety, in comparison with samples obtained from clinically healthy animals and using the GEA Dairy ProQ robotic milking system.Conclusion. In samples of milk obtained from sick cows using the Ley Astronaut robotic milking system, a microbiological study found a decrease in the sanitary quality of milk, which is an obstacle in the production of cheese.
{"title":"Characteristics of the sanitary quality of milk in sick cows with purulent inflammation of the uterus when using the GEA Dairy ProQ robotic milking system","authors":"A. Filatova, B. Tshivale, G. Nikitin, V. Avdeenko","doi":"10.31043/2410-2733-2023-1-96-103","DOIUrl":"https://doi.org/10.31043/2410-2733-2023-1-96-103","url":null,"abstract":"Purpose: to establish the role of reproductive pathologies in high-yielding cows in reducing the sanitary quality of milk at dairy enterprises using various milking systems.Materials and methods. The studies were carried out in high-tech dairy enterprises using GEA Dairy ProQ and Ley Astronaut robotic milking systems with cow productivity of more than 11 tons of milk per lactation. 4659 lactating cows were under observation. The selection of samples of uterine secretions, samples from the udder teats, the surface of the anogenital area, mammary gland and milk for bacteriological studies was carried out sterile, then they were sown on nutrient media.Results. The contents of the uterus with pyometra in 80% are contaminated with microorganisms: S. dysgalactiae – 29.1%, S. aureus – 26.4%, E. coli – 24.2%, and S. agalactiae – 20.3%. In 12% of cows, the microflora was isolated in monoculture. In samples of milk obtained from sick cows with uterine pathology using Ley Astronaut robotic milking, it showed 1.72 times lower sanitary safety, in comparison with samples obtained from clinically healthy animals and using the GEA Dairy ProQ robotic milking system.Conclusion. In samples of milk obtained from sick cows using the Ley Astronaut robotic milking system, a microbiological study found a decrease in the sanitary quality of milk, which is an obstacle in the production of cheese.","PeriodicalId":346303,"journal":{"name":"Genetics and breeding of animals","volume":"42 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114521381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-18DOI: 10.31043/2410-2733-2023-1-104-107
B. Semenov, G. Husainova, T. Kuznetsova, E. Finageev
Purpose: diagnosis of the disease and the subsequent surgical treatment of the Golstinsky breed bullshit in connection with a violation of the development of the testis.Materials and methods. The object of the study is the Golstein goby, which was born on a farm in Thuringia and then was contained in the general group (15 calves) on a deep straw litter. We carried out an early diagnosis of an animal disease aged 2 weeks, with a mass of 55 kg. During the clinical study, the condition of the calf was recorded as good, he actively took food, the clinical indicators were within normal limits, however, an increase in the volume of the scrotum that was inappropriate for its age was observed, and left -handed cryptorchidism was established. On the palpation of the scrotum, a slight soreness was noted, an increase in the right tested to 5 cm in length, 3 cm in width, the density of the shells of the testis and the fluctuation of the parenchyma. The left seed was not available for research due to its abdominal location. Since there was a rapid increase in the right seed, it was decided to carry out a unilateral castration of a bull to prevent the further development of the pathological process.Results. Castration was carried out with a bloody closed way on a firm ligature. The remote organ in the section did not have a clearly expressed parenchyma, but contained many cystic formations with transparent, liquid contents, in some cysts the contents were an admixture of blood. As a result of the surgical treatment of the bull, a pathologically changed testicist was removed, the second testis, which is abdominal, was left. To date, this disease is not fully studied and requires further thorough studies.
{"title":"Polycystic testis in bull of Holstein Breed","authors":"B. Semenov, G. Husainova, T. Kuznetsova, E. Finageev","doi":"10.31043/2410-2733-2023-1-104-107","DOIUrl":"https://doi.org/10.31043/2410-2733-2023-1-104-107","url":null,"abstract":"Purpose: diagnosis of the disease and the subsequent surgical treatment of the Golstinsky breed bullshit in connection with a violation of the development of the testis.Materials and methods. The object of the study is the Golstein goby, which was born on a farm in Thuringia and then was contained in the general group (15 calves) on a deep straw litter. We carried out an early diagnosis of an animal disease aged 2 weeks, with a mass of 55 kg. During the clinical study, the condition of the calf was recorded as good, he actively took food, the clinical indicators were within normal limits, however, an increase in the volume of the scrotum that was inappropriate for its age was observed, and left -handed cryptorchidism was established. On the palpation of the scrotum, a slight soreness was noted, an increase in the right tested to 5 cm in length, 3 cm in width, the density of the shells of the testis and the fluctuation of the parenchyma. The left seed was not available for research due to its abdominal location. Since there was a rapid increase in the right seed, it was decided to carry out a unilateral castration of a bull to prevent the further development of the pathological process.Results. Castration was carried out with a bloody closed way on a firm ligature. The remote organ in the section did not have a clearly expressed parenchyma, but contained many cystic formations with transparent, liquid contents, in some cysts the contents were an admixture of blood. As a result of the surgical treatment of the bull, a pathologically changed testicist was removed, the second testis, which is abdominal, was left. To date, this disease is not fully studied and requires further thorough studies.","PeriodicalId":346303,"journal":{"name":"Genetics and breeding of animals","volume":"50 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115980054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-18DOI: 10.31043/2410-2733-2023-1-65-74
S. Filatova, O. Sergeeva
Purpose: identification of plant potential, ecological and economic suitability of deer pastures of the forest-tundra zone located in the zone of anthropogenic influenceMaterial and methods. Ground geobotanical survey of the vegetation cover of deer pastures was carried out by route method in accordance with the method of geobotanical studies. The ecological and economic value of each type of pasture was determined based on the data of the ecological and economic classification of deer pastures in the north.Results. 25 species of reindeer pastures are described on the right bank of the Norilsk River in the northwestern part of the Putorana Plateau. On the basis of signs of uniformity in the structure of vegetation (proximity of species composition, similarity of vertical and horizontal structures), they are combined into 4 types: tundra, swamps, shrubs and light forests. Tundra (30.9%) and shrub (27.6%) types of pastures dominate. The most important feature is the active participation in the structure of phytocenoses of shrubs (Betula nana s. str, Salix glauca s. str, S. lanata s. str.) and shrubs (Ledum palustre. Vaccinium uliginosum s. str, V. vitis-idaea s. str.). Green fodder is most nutritious in the first half of summer. The nutritional value of fruticose lichens does not change during the year. Reindeer pastures with lichen cover and high (from 5 to 12 o-days/ha) reindeer capacity during the snowy period occupy insignificant (24.3%) areas. Basically, these are willow and dwarf dwarf shrub-lichen tundras and larch sparse forests of dwarf-shrub moss-lichen. The participation of lichens in the ground cover of plant communities does not exceed 25–35%. The following lichen species have been recorded: Cladonia arbuscula, C. stellaris, C. rangiferina, C. cornuta, C. deformis, C. mitis, Cetraria cucullata, and C. islandica. The most widespread species of the genus Cetraria: Cetraria islandica and C. cucullata, with a clear advantage of the latter. The main forage species of the genus Cladonia (Cladonia arbuscula, C. stellaris, C. rangiferina) are not widely distributed. Pastures with a high (from 9 o-days/ha to 14 o-days/ha) reindeer capacity in the summer, early autumn and late spring periods prevail in the study area. Basically, these are communities of shrub and marsh types. Shrubs (Salix glauca s. str, S. lanata s. str., S. hastata, Betula nana s. str.), shrubs (Salix reticulata, Vaccinium vitis-idaea s. str.) and forbs (species of the genera Petasites, Pedicularis, Astragalus, Hedysarum, Equisetum, Bistorta).
{"title":"Plant potential and ecological and economic suitability of reindeer pastures on the right bank of the Norilsk River","authors":"S. Filatova, O. Sergeeva","doi":"10.31043/2410-2733-2023-1-65-74","DOIUrl":"https://doi.org/10.31043/2410-2733-2023-1-65-74","url":null,"abstract":"Purpose: identification of plant potential, ecological and economic suitability of deer pastures of the forest-tundra zone located in the zone of anthropogenic influenceMaterial and methods. Ground geobotanical survey of the vegetation cover of deer pastures was carried out by route method in accordance with the method of geobotanical studies. The ecological and economic value of each type of pasture was determined based on the data of the ecological and economic classification of deer pastures in the north.Results. 25 species of reindeer pastures are described on the right bank of the Norilsk River in the northwestern part of the Putorana Plateau. On the basis of signs of uniformity in the structure of vegetation (proximity of species composition, similarity of vertical and horizontal structures), they are combined into 4 types: tundra, swamps, shrubs and light forests. Tundra (30.9%) and shrub (27.6%) types of pastures dominate. The most important feature is the active participation in the structure of phytocenoses of shrubs (Betula nana s. str, Salix glauca s. str, S. lanata s. str.) and shrubs (Ledum palustre. Vaccinium uliginosum s. str, V. vitis-idaea s. str.). Green fodder is most nutritious in the first half of summer. The nutritional value of fruticose lichens does not change during the year. Reindeer pastures with lichen cover and high (from 5 to 12 o-days/ha) reindeer capacity during the snowy period occupy insignificant (24.3%) areas. Basically, these are willow and dwarf dwarf shrub-lichen tundras and larch sparse forests of dwarf-shrub moss-lichen. The participation of lichens in the ground cover of plant communities does not exceed 25–35%. The following lichen species have been recorded: Cladonia arbuscula, C. stellaris, C. rangiferina, C. cornuta, C. deformis, C. mitis, Cetraria cucullata, and C. islandica. The most widespread species of the genus Cetraria: Cetraria islandica and C. cucullata, with a clear advantage of the latter. The main forage species of the genus Cladonia (Cladonia arbuscula, C. stellaris, C. rangiferina) are not widely distributed. Pastures with a high (from 9 o-days/ha to 14 o-days/ha) reindeer capacity in the summer, early autumn and late spring periods prevail in the study area. Basically, these are communities of shrub and marsh types. Shrubs (Salix glauca s. str, S. lanata s. str., S. hastata, Betula nana s. str.), shrubs (Salix reticulata, Vaccinium vitis-idaea s. str.) and forbs (species of the genera Petasites, Pedicularis, Astragalus, Hedysarum, Equisetum, Bistorta).","PeriodicalId":346303,"journal":{"name":"Genetics and breeding of animals","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129614466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-18DOI: 10.31043/2410-2733-2023-1-84-88
P. Pestis, L. Tanana
The paper presents the results of studying the associated effect of complex genotypes for the genes diacylglycerol O-acyl transferase 1 (DGAT1), somatotropin (GH), prolactin (PRL) and beta-lactoglobulin (BLG) on the milk productivity of Holstein dairy cattle of domestic selection. The object of research was cattle and biological material (ear pluck) from cows of Holstein dairy cattle of domestic selection, contained in the agricultural production cooperative named after I.P. Senko, Grodno region. DNA genotyping of animals for the genes of diacylglycerol O-acyl transferase 1 (DGAT1), somatotropin (GH), prolactin (PRL), and beta-lactoglobulin (BLG) was performed using the polymerase chain reaction (PCR) method and restriction fragment length polymorphism ( RFLP). The results of studies on the assessment of the associated effect of complex genotypes for the genes diacylglycerol O-acyl transferase 1 (DGAT1), somatotropin (GH), prolactin (PRL) and beta-lactoglobulin (BLG) on the milk productivity of Holstein cows of dairy cattle domestic selection showed that in terms of the mass fraction of fat and the amount of milk fat in milk, in most cases, the highest rates were in animals with the complex genotype.
{"title":"Slaughter indicators of purebred Hereford bulls depending on the genotypes of the gene GDP-l-fucosynthetase (TSTA3)","authors":"P. Pestis, L. Tanana","doi":"10.31043/2410-2733-2023-1-84-88","DOIUrl":"https://doi.org/10.31043/2410-2733-2023-1-84-88","url":null,"abstract":"The paper presents the results of studying the associated effect of complex genotypes for the genes diacylglycerol O-acyl transferase 1 (DGAT1), somatotropin (GH), prolactin (PRL) and beta-lactoglobulin (BLG) on the milk productivity of Holstein dairy cattle of domestic selection. The object of research was cattle and biological material (ear pluck) from cows of Holstein dairy cattle of domestic selection, contained in the agricultural production cooperative named after I.P. Senko, Grodno region. DNA genotyping of animals for the genes of diacylglycerol O-acyl transferase 1 (DGAT1), somatotropin (GH), prolactin (PRL), and beta-lactoglobulin (BLG) was performed using the polymerase chain reaction (PCR) method and restriction fragment length polymorphism ( RFLP). The results of studies on the assessment of the associated effect of complex genotypes for the genes diacylglycerol O-acyl transferase 1 (DGAT1), somatotropin (GH), prolactin (PRL) and beta-lactoglobulin (BLG) on the milk productivity of Holstein cows of dairy cattle domestic selection showed that in terms of the mass fraction of fat and the amount of milk fat in milk, in most cases, the highest rates were in animals with the complex genotype.","PeriodicalId":346303,"journal":{"name":"Genetics and breeding of animals","volume":"51 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132329518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-17DOI: 10.31043/2410-2733-2023-1-16-21
N. Panova
Purpose: To study the mechanisms of reducing the concentration of estrogens in the mammary gland.Materials and methods. In the experiment, breast preparations obtained from lactating rats were used. Estradiol receptors were detected using autoradiographic study and biochemical method in the cytosolic fraction. The specificity of estradiol binding was determined as the difference between the total binding during incubation in a medium containing 10-9 M ³H-17 β estradiol and binding during incubation in a medium containing, in addition to the labeled hormone, a 100-fold excess of unlabeled estradiol. During the washing process, structures with significant affinity for the hormone were revealed.Results. As a result of the study, the reception of estradiol by the cells of the alveolar part of the mammary gland during lactation was established. In the alveoli of the mammary gland, there is an intense binding of labeled estradiol by glandular cells and leukocytes. At high values of specific binding of estradiol, intensely labeled cells are located in the form of a close cluster. An indicator of the membrane binding of estradiol is the numerous silver granules accumulating over the cell border.Conclusion. The study made it possible to analyze the reception of estradiol in hormone-dependent tissue (mammary gland). Leukocyte cells have estrogen receptors, which reduce the level of the hormone as a result of lactation. In the mammary gland, there is a mechanism for reducing the concentration of estrogens. The ability of leukocyte receptor binding allows us to speak about the role of milk and colostrum cells in reducing the level of estrogens, which is one of the important factors in reducing the development of pathological processes in breast tissues.
{"title":"Influence of the receptor apparatus of the mammary gland on the content of estrogens during the lactation process in rats","authors":"N. Panova","doi":"10.31043/2410-2733-2023-1-16-21","DOIUrl":"https://doi.org/10.31043/2410-2733-2023-1-16-21","url":null,"abstract":"Purpose: To study the mechanisms of reducing the concentration of estrogens in the mammary gland.Materials and methods. In the experiment, breast preparations obtained from lactating rats were used. Estradiol receptors were detected using autoradiographic study and biochemical method in the cytosolic fraction. The specificity of estradiol binding was determined as the difference between the total binding during incubation in a medium containing 10-9 M ³H-17 β estradiol and binding during incubation in a medium containing, in addition to the labeled hormone, a 100-fold excess of unlabeled estradiol. During the washing process, structures with significant affinity for the hormone were revealed.Results. As a result of the study, the reception of estradiol by the cells of the alveolar part of the mammary gland during lactation was established. In the alveoli of the mammary gland, there is an intense binding of labeled estradiol by glandular cells and leukocytes. At high values of specific binding of estradiol, intensely labeled cells are located in the form of a close cluster. An indicator of the membrane binding of estradiol is the numerous silver granules accumulating over the cell border.Conclusion. The study made it possible to analyze the reception of estradiol in hormone-dependent tissue (mammary gland). Leukocyte cells have estrogen receptors, which reduce the level of the hormone as a result of lactation. In the mammary gland, there is a mechanism for reducing the concentration of estrogens. The ability of leukocyte receptor binding allows us to speak about the role of milk and colostrum cells in reducing the level of estrogens, which is one of the important factors in reducing the development of pathological processes in breast tissues.","PeriodicalId":346303,"journal":{"name":"Genetics and breeding of animals","volume":"33 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117133584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-17DOI: 10.31043/2410-2733-2023-1-38-44
Ch. Gaytov, V. Gappoeva, I. Ktsoeva, R. Temiraev, S. Kozyrev
Purpose: the study of the exposure of the prototype of sagging and the phospholipid complex of lecithin for the intermediate exchange and enzymatic activity of the anti -radical protection of the body of quails in the presence of a subtoxic dose of nitrates in the compound feeds.Materials and methods. To carry out a scientific and production experiment, meat caves "Pharaoh" were used. From per diem, 4 groups of experimental birds of 50 goals in each were formed on the principle of groups of groups analogues. Compound feeds used for feeding are quittered, grown for meat, were prosperous in the presence of nitrates. With this in mind, for the purity of the experiment, they additionally introduced sodium nitrate in a dose of 40 g/t feed in their composition for all groups in the role of the source of these xenobiotics. At the same time, the presence of nitrates in the diet in a subtoxic dose was ensured. A sagitol’s sagitot at a dose of 1250 g/t, for the 3rd group-1000 g/t lacitin, for the 4th-1250 g/t of the sagitol test + 1000 g/t Lecin, was added to the feed. The 1st group was controlled - for birds of these groups in compensation, sagitol and lecithin were not added to determine the effectiveness of denitrification studied the basic morphological and biochemical parameters of blood and the activity of enzymes of anti -radical protection of the body of quails (5 heads in the group) aged 42 days.Results. In the course of the studies, it was first established that joint supplements in the diets of produces of produces of sagol at a dose of 1.25 kg/t of feed and lecithin at a dose of 1.0 kg/t food contributed to effective denitrification in the body of the plyona breed. This was manifested in a reliable (p <0.05) increase in the bird of the 3rd experimental group regarding control in the liquid internal environment of the number of red blood cells and hemoglobin, as well as in a decrease in the concentration of metghemoglobin by 45.17% (p <0.05) . With joint additives in the diets of probiotic and lecithin, an improvement in the state of natural resistance and antioxidant protection of the poultry body was observed, which was manifested in an increase in the quails of the 3rd experimental group regarding control of lysozymic activity by 2.99% (p <0.05), bacticipid by 11.09% (p <0.05) and the activity of glutathioneperoxidase - by 43.22% (p <0.05) with a decrease in catalase activity - by 17.46% (p <0.05). Under the influence of these drugs, the quails of 3 of the experimental group observed the best level of denitrification, which in the blood serum increased the amount of uric acid by 42.56% (p <0.05) due to a decrease in nitrates by 50.54% (p <0 , 05) and nitrites - by 60.55% (p <0.05).
{"title":"The state of intermediate metabolism and antioxidant protection of the organism of quail during denitrifation","authors":"Ch. Gaytov, V. Gappoeva, I. Ktsoeva, R. Temiraev, S. Kozyrev","doi":"10.31043/2410-2733-2023-1-38-44","DOIUrl":"https://doi.org/10.31043/2410-2733-2023-1-38-44","url":null,"abstract":"Purpose: the study of the exposure of the prototype of sagging and the phospholipid complex of lecithin for the intermediate exchange and enzymatic activity of the anti -radical protection of the body of quails in the presence of a subtoxic dose of nitrates in the compound feeds.Materials and methods. To carry out a scientific and production experiment, meat caves \"Pharaoh\" were used. From per diem, 4 groups of experimental birds of 50 goals in each were formed on the principle of groups of groups analogues. Compound feeds used for feeding are quittered, grown for meat, were prosperous in the presence of nitrates. With this in mind, for the purity of the experiment, they additionally introduced sodium nitrate in a dose of 40 g/t feed in their composition for all groups in the role of the source of these xenobiotics. At the same time, the presence of nitrates in the diet in a subtoxic dose was ensured. A sagitol’s sagitot at a dose of 1250 g/t, for the 3rd group-1000 g/t lacitin, for the 4th-1250 g/t of the sagitol test + 1000 g/t Lecin, was added to the feed. The 1st group was controlled - for birds of these groups in compensation, sagitol and lecithin were not added to determine the effectiveness of denitrification studied the basic morphological and biochemical parameters of blood and the activity of enzymes of anti -radical protection of the body of quails (5 heads in the group) aged 42 days.Results. In the course of the studies, it was first established that joint supplements in the diets of produces of produces of sagol at a dose of 1.25 kg/t of feed and lecithin at a dose of 1.0 kg/t food contributed to effective denitrification in the body of the plyona breed. This was manifested in a reliable (p <0.05) increase in the bird of the 3rd experimental group regarding control in the liquid internal environment of the number of red blood cells and hemoglobin, as well as in a decrease in the concentration of metghemoglobin by 45.17% (p <0.05) . With joint additives in the diets of probiotic and lecithin, an improvement in the state of natural resistance and antioxidant protection of the poultry body was observed, which was manifested in an increase in the quails of the 3rd experimental group regarding control of lysozymic activity by 2.99% (p <0.05), bacticipid by 11.09% (p <0.05) and the activity of glutathioneperoxidase - by 43.22% (p <0.05) with a decrease in catalase activity - by 17.46% (p <0.05). Under the influence of these drugs, the quails of 3 of the experimental group observed the best level of denitrification, which in the blood serum increased the amount of uric acid by 42.56% (p <0.05) due to a decrease in nitrates by 50.54% (p <0 , 05) and nitrites - by 60.55% (p <0.05).","PeriodicalId":346303,"journal":{"name":"Genetics and breeding of animals","volume":"77 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133848209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-17DOI: 10.31043/2410-2733-2023-1-5-15
V. Maksimov, O. Ivantsova, A. Deltsov, A. Frenk
Purpose: to study the effect of complex additives based on a protein hydrolyzate together with an iron-containing preparation on the metabolism of pregnant goats of the Saanen breed of the first lactation in the most stressful functional states of the body in the life of a female (the last stage of pregnancy and the postpartum period) according to the physiological, biochemical and hormonal status of the blood of animals, grown in an industrial complex.Materials and methods. Experimental studies were carried out on Saanen goats from the moment of 110 days (days) of their pregnancy upon reaching 30 days. offspring obtained from the studied animals, in the conditions of an industrial complex. In the experiment, 24 pregnant Saanen goats (first pregnancy) were used, of which 8 goats were combined into a control group, and 16 into two experimental groups, divided equally. The content and feeding of goats and kids obtained from them was zootechnically determined. In addition to the diet, the experimental animals received a biological supplement based on a protein hydrolyzate, 40 ml/head/day. and an iron-containing preparation, 10 ml/animal/day. One experimental group received the preparation "Abiopeptid" + "Bio-iron with microelements", the other - "Abiotonic" + "Bio-iron with microelements". All dietary supplements were administered to animals orally through individual numbered syringes of 20 and 10 ml. Animals received dietary supplements continuously from 110 days of pregnancy until the resulting offspring reaches 30 days. (i.e. 70 days - 40 during gestation and 30 after delivery). To assess the state of control and experimental goats, blood was taken (at 150 days of gestation and after 30 days from birth), followed by biochemical and hormonal analysis.Results. With regard to the use of bioadditives based on protein hydrolyzate in goats of the last stage of pregnancy and the beginning of the first lactation, in terms of the parameters of the physiological, biochemical and hormonal blood status, it should be noted that the beneficial properties of bioadditives aimed at enhancing the catabolic processes of the body will be effective only if the animals are provided with favorable micro- and macroclimatic parameters, in particular, a sufficient and complete diet. In case of violation of the conditions of detention, the effectiveness of biologically active substances will be provided at the expense of the body's own reserves. It should be noted the effectiveness of the iron-containing preparation "Bio-iron", used in feeding goats that are at the late stage of the pregnancy of the first pregnancy and during the milking period of the first lactation.
目的:研究以蛋白质水解物为基础的复合添加剂与含铁制剂结合，根据工业园区动物血液的生理、生化和激素状况，研究在母羊生命中身体最紧张的功能状态(妊娠后期和产后)下，首次泌乳的Saanen品种妊娠山羊代谢的影响。材料和方法。试验研究从妊娠第110天(天)至30天开始对萨宁山羊进行。在工业园区的条件下，从被研究动物身上获得的后代。试验选用24只首次妊娠的妊娠沙嫩山羊，其中8只山羊分为对照组，16只山羊分为2个试验组，每组平均分配。用动物技术测定所得山羊和幼山羊的含量和饲料。在饲粮的基础上，实验动物还添加了以蛋白质水解物为基础的生物补充剂，40 ml/头/天。含铁制剂，10ml /只动物/天。实验组采用“Abiopeptid”+“Bio-iron with microelements”制剂，实验组采用“Abiotonic”+“Bio-iron with microelements”制剂。所有的膳食补充剂通过20和10毫升的单独编号注射器口服给药。动物从怀孕110天开始连续服用膳食补充剂，直到所生后代30天。(即妊娠期70天至40天，分娩后30天)。为了评估对照山羊和实验山羊的状态，分别在妊娠150天和出生后30天采血，然后进行生化和激素分析。关于在妊娠末期和哺乳期初的山羊中使用以蛋白水解物为基础的生物添加剂，就生理生化和激素血液状态的参数而言，应该注意的是，生物添加剂旨在增强身体分解代谢过程的有益特性只有在动物具有有利的微观和宏观气候参数的情况下才能有效，特别是:充足和完整的饮食。在违反拘留条件的情况下，生物活性物质的有效性将以牺牲人体自身储备为代价。应该注意的是，含铁制剂“生物铁”的有效性，用于喂养第一次怀孕的妊娠后期和第一次哺乳的挤奶期的山羊。
{"title":"Metabolic peculiarities of pregnant goats of the Saanen breed in the last stage of pregnancy and postpartum period under the influence of stimulated BAS","authors":"V. Maksimov, O. Ivantsova, A. Deltsov, A. Frenk","doi":"10.31043/2410-2733-2023-1-5-15","DOIUrl":"https://doi.org/10.31043/2410-2733-2023-1-5-15","url":null,"abstract":"Purpose: to study the effect of complex additives based on a protein hydrolyzate together with an iron-containing preparation on the metabolism of pregnant goats of the Saanen breed of the first lactation in the most stressful functional states of the body in the life of a female (the last stage of pregnancy and the postpartum period) according to the physiological, biochemical and hormonal status of the blood of animals, grown in an industrial complex.Materials and methods. Experimental studies were carried out on Saanen goats from the moment of 110 days (days) of their pregnancy upon reaching 30 days. offspring obtained from the studied animals, in the conditions of an industrial complex. In the experiment, 24 pregnant Saanen goats (first pregnancy) were used, of which 8 goats were combined into a control group, and 16 into two experimental groups, divided equally. The content and feeding of goats and kids obtained from them was zootechnically determined. In addition to the diet, the experimental animals received a biological supplement based on a protein hydrolyzate, 40 ml/head/day. and an iron-containing preparation, 10 ml/animal/day. One experimental group received the preparation \"Abiopeptid\" + \"Bio-iron with microelements\", the other - \"Abiotonic\" + \"Bio-iron with microelements\". All dietary supplements were administered to animals orally through individual numbered syringes of 20 and 10 ml. Animals received dietary supplements continuously from 110 days of pregnancy until the resulting offspring reaches 30 days. (i.e. 70 days - 40 during gestation and 30 after delivery). To assess the state of control and experimental goats, blood was taken (at 150 days of gestation and after 30 days from birth), followed by biochemical and hormonal analysis.Results. With regard to the use of bioadditives based on protein hydrolyzate in goats of the last stage of pregnancy and the beginning of the first lactation, in terms of the parameters of the physiological, biochemical and hormonal blood status, it should be noted that the beneficial properties of bioadditives aimed at enhancing the catabolic processes of the body will be effective only if the animals are provided with favorable micro- and macroclimatic parameters, in particular, a sufficient and complete diet. In case of violation of the conditions of detention, the effectiveness of biologically active substances will be provided at the expense of the body's own reserves. It should be noted the effectiveness of the iron-containing preparation \"Bio-iron\", used in feeding goats that are at the late stage of the pregnancy of the first pregnancy and during the milking period of the first lactation.","PeriodicalId":346303,"journal":{"name":"Genetics and breeding of animals","volume":"29 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116887837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-17DOI: 10.31043/2410-2733-2023-1-22-25
V. Eremenko, E. Rothmistrykaya
Purpose: to study the dynamics of insulin in different periods of the flow of different breeds: a golstinized black-and-haired, Simmental, Aberdeen-Angus and Knieuted Netele (Simmental X Aberdin-Anguskaya).Materials and methods. The object of research was not there was a golstinized Black-and-white, Simmental, Aberdin-Anguskaya and crossbreeds (golstinized Black-and-white x Simmental) in each group in each group. Explust animals were grown in the same conditions. During all periods of experience, Kalles took blood samples until morning feeding. In blood samples, the level of insulin was determined by the immunofortment method. The level of feeding of the heels corresponded to the zootechnical norms.Results. Before insemination, the level of insulin in black-and-chilled heifers was 11.0 ± 0.5, in the Simmental-12.1 ± 0.6, in Aberdin-Angus-12.8 ± 0.6, in the combed-13.1 ± 0 , 7 μma/ml. In the future, with an increase in the duration of animals, the concentration of this hormone in the blood increased and by the 5th month the concentration of insulin was noted as the maximum for the entire period. In black-and-playing, in this period, the level of insulin increased to 12.3 ± 0.6, in the Simmental to 13.0 ± 0.8, in the Aberdine-Anguski to 14.1 ± 0.7, in Monnie, up to 14, 4 ± 0.7 μm/ml. By the end, the level of insulin in the blood of the miles was significantly reduced in black-and-playing noles by 9 months of age, it was 10.2 ± 0.6, in the Simmental-10.5 ± 0.7, in the Aberdine-Angus-11.4 ± 0. , 7, in the Knieut - 12.6 ± 0.7 mkm/ml. In all periods, the relatively low level of insulin was marked by black-festivities in relation to the compared breeds: Simmental, Aberdeen-Anguska and Knight Netel.
{"title":"The dynamics of the concentration of insulin in the blood of a heifer of different breeds","authors":"V. Eremenko, E. Rothmistrykaya","doi":"10.31043/2410-2733-2023-1-22-25","DOIUrl":"https://doi.org/10.31043/2410-2733-2023-1-22-25","url":null,"abstract":"Purpose: to study the dynamics of insulin in different periods of the flow of different breeds: a golstinized black-and-haired, Simmental, Aberdeen-Angus and Knieuted Netele (Simmental X Aberdin-Anguskaya).Materials and methods. The object of research was not there was a golstinized Black-and-white, Simmental, Aberdin-Anguskaya and crossbreeds (golstinized Black-and-white x Simmental) in each group in each group. Explust animals were grown in the same conditions. During all periods of experience, Kalles took blood samples until morning feeding. In blood samples, the level of insulin was determined by the immunofortment method. The level of feeding of the heels corresponded to the zootechnical norms.Results. Before insemination, the level of insulin in black-and-chilled heifers was 11.0 ± 0.5, in the Simmental-12.1 ± 0.6, in Aberdin-Angus-12.8 ± 0.6, in the combed-13.1 ± 0 , 7 μma/ml. In the future, with an increase in the duration of animals, the concentration of this hormone in the blood increased and by the 5th month the concentration of insulin was noted as the maximum for the entire period. In black-and-playing, in this period, the level of insulin increased to 12.3 ± 0.6, in the Simmental to 13.0 ± 0.8, in the Aberdine-Anguski to 14.1 ± 0.7, in Monnie, up to 14, 4 ± 0.7 μm/ml. By the end, the level of insulin in the blood of the miles was significantly reduced in black-and-playing noles by 9 months of age, it was 10.2 ± 0.6, in the Simmental-10.5 ± 0.7, in the Aberdine-Angus-11.4 ± 0. , 7, in the Knieut - 12.6 ± 0.7 mkm/ml. In all periods, the relatively low level of insulin was marked by black-festivities in relation to the compared breeds: Simmental, Aberdeen-Anguska and Knight Netel.","PeriodicalId":346303,"journal":{"name":"Genetics and breeding of animals","volume":"365 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133708700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-17DOI: 10.31043/2410-2733-2023-1-32-37
O. Pavlova, O. Tulaeva, O. Gulenko, O. Bukareva
Purpose: to study the dynamics of catalase activity in the blood and liver liver tissues with oxidative stress caused by the modeling of dyslipoproteinemia with the subsequent mechanical trauma of the skeletal muscle.Materials and methods. The study was conducted on white, unconventional sexual healthy rags of six months of age, weighing 200-230 grams, in the amount of 120 pieces contained in standard conditions of vivarium. All animals were divided into four groups, 30 animals in each group. Animals of the first group are intact rats to which no effects were applied. Animals of the second group (regeneration control) had a mechanical dissection in the area of the middle third of the calf muscle of the posterior limb. Animals of 1 and 2 groups were on the standard diet of Vivaria and had free access to water and food. The animals of the third and fourth groups were first modeled by dyslipoprotein for 63 days. All this time, animals received a high -carb and high -fat diet with a weight of dry substances increased up to 30 % and replacement of drinking water with a 20 % fructose solution. After the specified time, the animals of the fourth group were injured by the middle third of the calf muscle of the posterior limb. The activity of catalase was investigated in animals before the start of the experiment, as well as on 1, 3, 5, 14, 21 days of experience according to the standard methodology of Queen M. A. The capture of the material and the removal of rats from the experiment was carried out with accurate observance of all ethical norms applicable to laboratory animals . Under etheric anesthesia, a blood fence was made, and then the liver fence, which was washed with physiological solution and frozen. Then, by mechanical grinding of fabrics weighing 1 g and mixing with 9 ml of Tris Bufer (pH 7.4), homogenates were prepared in an automatic homogen at a speed of 5,000 rpm in a vessel with double walls, constantly cooled by running water.Results. The mechanical trauma of the calf muscle and dyslipoprotemia contributed to the intensification of oxidative processes at the local and systemic levels of the body, but the combination of these facts was characterized by extremely intense oxidative stress and led to a decrease in the activity of catalase in the blood and liver tissues.
{"title":"Study of changes in catalase activity in blood and liver of rats with experimentally induced dyslipoproteinemia against the background of mechanical damage to skeletal muscle","authors":"O. Pavlova, O. Tulaeva, O. Gulenko, O. Bukareva","doi":"10.31043/2410-2733-2023-1-32-37","DOIUrl":"https://doi.org/10.31043/2410-2733-2023-1-32-37","url":null,"abstract":"Purpose: to study the dynamics of catalase activity in the blood and liver liver tissues with oxidative stress caused by the modeling of dyslipoproteinemia with the subsequent mechanical trauma of the skeletal muscle.Materials and methods. The study was conducted on white, unconventional sexual healthy rags of six months of age, weighing 200-230 grams, in the amount of 120 pieces contained in standard conditions of vivarium. All animals were divided into four groups, 30 animals in each group. Animals of the first group are intact rats to which no effects were applied. Animals of the second group (regeneration control) had a mechanical dissection in the area of the middle third of the calf muscle of the posterior limb. Animals of 1 and 2 groups were on the standard diet of Vivaria and had free access to water and food. The animals of the third and fourth groups were first modeled by dyslipoprotein for 63 days. All this time, animals received a high -carb and high -fat diet with a weight of dry substances increased up to 30 % and replacement of drinking water with a 20 % fructose solution. After the specified time, the animals of the fourth group were injured by the middle third of the calf muscle of the posterior limb. The activity of catalase was investigated in animals before the start of the experiment, as well as on 1, 3, 5, 14, 21 days of experience according to the standard methodology of Queen M. A. The capture of the material and the removal of rats from the experiment was carried out with accurate observance of all ethical norms applicable to laboratory animals . Under etheric anesthesia, a blood fence was made, and then the liver fence, which was washed with physiological solution and frozen. Then, by mechanical grinding of fabrics weighing 1 g and mixing with 9 ml of Tris Bufer (pH 7.4), homogenates were prepared in an automatic homogen at a speed of 5,000 rpm in a vessel with double walls, constantly cooled by running water.Results. The mechanical trauma of the calf muscle and dyslipoprotemia contributed to the intensification of oxidative processes at the local and systemic levels of the body, but the combination of these facts was characterized by extremely intense oxidative stress and led to a decrease in the activity of catalase in the blood and liver tissues.","PeriodicalId":346303,"journal":{"name":"Genetics and breeding of animals","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121009697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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