Pub Date : 2004-12-15DOI: 10.1109/APBP.2004.1412279
Lee‐Jen Chen
Photodynamic therapy is an effective treatment for wet form macular degeneration. Besides fluorescein angiography, optical coherence tomography can monitor fluid level lesion size changes and detect retinal pigment epithelium detachment after treatment.
{"title":"Optical coherence tomography (OCT) findings following photodynamic therapy (PDT) in ophthalmology","authors":"Lee‐Jen Chen","doi":"10.1109/APBP.2004.1412279","DOIUrl":"https://doi.org/10.1109/APBP.2004.1412279","url":null,"abstract":"Photodynamic therapy is an effective treatment for wet form macular degeneration. Besides fluorescein angiography, optical coherence tomography can monitor fluid level lesion size changes and detect retinal pigment epithelium detachment after treatment.","PeriodicalId":346624,"journal":{"name":"The Second Asian and Pacific Rim Symposium on Biophotonics, 2004. APBP 2004.","volume":"109 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2004-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129656024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2004-12-15DOI: 10.1109/APBP.2004.1412369
P. Wei, P. Huang, Yi-Chun Chen
A new optical reading system that can read the fluorescent signals of microarrays on glass slides with large detection area and high sensitivity is proposed. This system uses gratings (1200 g/mm) with several micron thick PDMS film on surface. Normally incident laser was coupled into a thin glass slide by grating coupling and confined in the slide due to the total internal reflection (TIR). The TIR generates evanescent planar wave on the glass surface. The evanescent wave was employed to excite the fluorescent signals in the microarrays. The method takes the advantages of low excitation background, large and uniform detection area.
{"title":"Evanescent planar wave system for reading DNA microarrays on thin glass slides","authors":"P. Wei, P. Huang, Yi-Chun Chen","doi":"10.1109/APBP.2004.1412369","DOIUrl":"https://doi.org/10.1109/APBP.2004.1412369","url":null,"abstract":"A new optical reading system that can read the fluorescent signals of microarrays on glass slides with large detection area and high sensitivity is proposed. This system uses gratings (1200 g/mm) with several micron thick PDMS film on surface. Normally incident laser was coupled into a thin glass slide by grating coupling and confined in the slide due to the total internal reflection (TIR). The TIR generates evanescent planar wave on the glass surface. The evanescent wave was employed to excite the fluorescent signals in the microarrays. The method takes the advantages of low excitation background, large and uniform detection area.","PeriodicalId":346624,"journal":{"name":"The Second Asian and Pacific Rim Symposium on Biophotonics, 2004. APBP 2004.","volume":"03 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2004-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129959591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2004-12-15DOI: 10.1109/APBP.2004.1412262
K. Kek, M. Samizo, N. Kudo, K. Yamamoto
We have developed a spatially resolved NIRS imaging system with novel optical probes and fast temporal resolution. Initial results showed the effectiveness of our system for real-time spatio-temporal analyses for muscle oxygenation during exercises.
{"title":"Real-time imaging of muscle oxygenation using spatially resolved near-infrared spectroscopy","authors":"K. Kek, M. Samizo, N. Kudo, K. Yamamoto","doi":"10.1109/APBP.2004.1412262","DOIUrl":"https://doi.org/10.1109/APBP.2004.1412262","url":null,"abstract":"We have developed a spatially resolved NIRS imaging system with novel optical probes and fast temporal resolution. Initial results showed the effectiveness of our system for real-time spatio-temporal analyses for muscle oxygenation during exercises.","PeriodicalId":346624,"journal":{"name":"The Second Asian and Pacific Rim Symposium on Biophotonics, 2004. APBP 2004.","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2004-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130570393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2004-12-15DOI: 10.1109/APBP.2004.1412361
H. Ho, S.Y. Wu, W. Law, Chinlon Lin
Surface plasmon resonance (SPR) is an effective tool for quantitative detection of binding reaction between biomolecules. Currently SPR sensors are mainly based on measuring the angular shift associated with biomolecular adhesion. In fact it has been shown that the phase jump across the SPR dip is very steep, and consequently better sensitivity may be achieved by measuring the optical phase. In this presentation, we report interferometric techniques we have developed in our laboratory for achieving SPR phase detection. We also demonstrate that the incorporation of differential phase detection can further improve the performance of SPR biosensors. Currently the experimental sensitivity of our differential phase setup is 5.48/spl times/10/sup -8/ refractive index unit (RIU), which is three times better than that reported from conventional angular systems.
{"title":"Application of differential phase detection for sensitivity improvement in surface plasmon resonance sensors","authors":"H. Ho, S.Y. Wu, W. Law, Chinlon Lin","doi":"10.1109/APBP.2004.1412361","DOIUrl":"https://doi.org/10.1109/APBP.2004.1412361","url":null,"abstract":"Surface plasmon resonance (SPR) is an effective tool for quantitative detection of binding reaction between biomolecules. Currently SPR sensors are mainly based on measuring the angular shift associated with biomolecular adhesion. In fact it has been shown that the phase jump across the SPR dip is very steep, and consequently better sensitivity may be achieved by measuring the optical phase. In this presentation, we report interferometric techniques we have developed in our laboratory for achieving SPR phase detection. We also demonstrate that the incorporation of differential phase detection can further improve the performance of SPR biosensors. Currently the experimental sensitivity of our differential phase setup is 5.48/spl times/10/sup -8/ refractive index unit (RIU), which is three times better than that reported from conventional angular systems.","PeriodicalId":346624,"journal":{"name":"The Second Asian and Pacific Rim Symposium on Biophotonics, 2004. APBP 2004.","volume":"51 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2004-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132272597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2004-12-15DOI: 10.1109/APBP.2004.1412261
Z. Feng
Raman spectroscopy was utilized to study surgical excision specimens of human breast and lymph node tissues from patients with leaking silicone bag-gel breast implants. This may offer a new method of detection to evaluate tissues.
{"title":"Raman spectroscopic detection of silicone leakage in human breast and lymph node tissues","authors":"Z. Feng","doi":"10.1109/APBP.2004.1412261","DOIUrl":"https://doi.org/10.1109/APBP.2004.1412261","url":null,"abstract":"Raman spectroscopy was utilized to study surgical excision specimens of human breast and lymph node tissues from patients with leaking silicone bag-gel breast implants. This may offer a new method of detection to evaluate tissues.","PeriodicalId":346624,"journal":{"name":"The Second Asian and Pacific Rim Symposium on Biophotonics, 2004. APBP 2004.","volume":"176 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2004-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123268444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2004-12-15DOI: 10.1109/APBP.2004.1412260
M. Fujii, K. Nakayama
We propose a Monte Carlo method which can simulate scattering of polarized light in sphere suspension and also present a basic system for 2-D angular pattern of the backscattering light from turbid media. The experimental results of polystyrene sphere suspension coincide well with the simulation results.
{"title":"Polarization measurement of backscattering from turbid media: simulation and experiment","authors":"M. Fujii, K. Nakayama","doi":"10.1109/APBP.2004.1412260","DOIUrl":"https://doi.org/10.1109/APBP.2004.1412260","url":null,"abstract":"We propose a Monte Carlo method which can simulate scattering of polarized light in sphere suspension and also present a basic system for 2-D angular pattern of the backscattering light from turbid media. The experimental results of polystyrene sphere suspension coincide well with the simulation results.","PeriodicalId":346624,"journal":{"name":"The Second Asian and Pacific Rim Symposium on Biophotonics, 2004. APBP 2004.","volume":"14 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2004-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126407884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2004-12-15DOI: 10.1109/APBP.2004.1412364
T. Sumiyoshi
Femtosecond lasers have been developed for biomedical applications.
飞秒激光器已被开发用于生物医学应用。
{"title":"Femtosecond lasers for medical applications","authors":"T. Sumiyoshi","doi":"10.1109/APBP.2004.1412364","DOIUrl":"https://doi.org/10.1109/APBP.2004.1412364","url":null,"abstract":"Femtosecond lasers have been developed for biomedical applications.","PeriodicalId":346624,"journal":{"name":"The Second Asian and Pacific Rim Symposium on Biophotonics, 2004. APBP 2004.","volume":"16 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2004-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122861780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2004-12-15DOI: 10.1109/APBP.2004.1412303
W. Hsu
Pediatric airway management is a life-threatening problem for children and always a challenge to pediatric otolaryngologists. Endoscopic techniques for evaluation and management of airway in pediatric patients have matured greatly in the past decade, especially with the introduction of Laser instrumentation. For example, to treat severe laryngomalacia, subglottic hemangioma, laryngeal papillomatosis, granuloma or web, subglottic or tracheal stenosis, bilateral vocal fold paralysis, tracheobronchial tumor etc. were all effective in selective cases. These developments have been proved that pediatric endoscopic laser surgery can be performed in the airway safely and effectively when a team approach is used. The appropriate choice of laser instruments, anesthesia access, post-operation intensive cares, cooperation and understanding of families were all essential factors for a successful endoscopic laser surgery in pediatric airway management.
{"title":"Endoscopic laser surgery in pediatric airway management","authors":"W. Hsu","doi":"10.1109/APBP.2004.1412303","DOIUrl":"https://doi.org/10.1109/APBP.2004.1412303","url":null,"abstract":"Pediatric airway management is a life-threatening problem for children and always a challenge to pediatric otolaryngologists. Endoscopic techniques for evaluation and management of airway in pediatric patients have matured greatly in the past decade, especially with the introduction of Laser instrumentation. For example, to treat severe laryngomalacia, subglottic hemangioma, laryngeal papillomatosis, granuloma or web, subglottic or tracheal stenosis, bilateral vocal fold paralysis, tracheobronchial tumor etc. were all effective in selective cases. These developments have been proved that pediatric endoscopic laser surgery can be performed in the airway safely and effectively when a team approach is used. The appropriate choice of laser instruments, anesthesia access, post-operation intensive cares, cooperation and understanding of families were all essential factors for a successful endoscopic laser surgery in pediatric airway management.","PeriodicalId":346624,"journal":{"name":"The Second Asian and Pacific Rim Symposium on Biophotonics, 2004. APBP 2004.","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2004-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132662932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2004-12-15DOI: 10.1109/APBP.2004.1412323
Keke Shang, Y. Liu
In this paper, we introduced an improved digital imaging measurement system on skin diseases diagnosis Then basing on analysis of a large amount of clinical cases on skin diseases, we corrected the HSV color space in order to analyze characteristics of the color on disease skin. On the end, we examined the meaning of three indexes through analysis of some clinical cases.
{"title":"Study on digital imaging colorimeter in dermatology","authors":"Keke Shang, Y. Liu","doi":"10.1109/APBP.2004.1412323","DOIUrl":"https://doi.org/10.1109/APBP.2004.1412323","url":null,"abstract":"In this paper, we introduced an improved digital imaging measurement system on skin diseases diagnosis Then basing on analysis of a large amount of clinical cases on skin diseases, we corrected the HSV color space in order to analyze characteristics of the color on disease skin. On the end, we examined the meaning of three indexes through analysis of some clinical cases.","PeriodicalId":346624,"journal":{"name":"The Second Asian and Pacific Rim Symposium on Biophotonics, 2004. APBP 2004.","volume":"53 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2004-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133997298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2004-12-15DOI: 10.1109/APBP.2004.1412292
Lin-Hung Wei, C. Chou, J. Su
Summary form only given. Photodynamic therapy (PDT) is a promising treatment modality that is being tested in the clinic for use in oncology. PDT requires three elements: light, a photosensitizer and oxygen. PDT-mediated oxidative stress elicits direct tumor cell damage and microvascular injury within exposed tumor. Microvasculature damage following PDT leads to a significant decrease in blood flow as well as severe and persistent tumor tissue hypoxia. Subsequently, tissue hypoxia can induce a plethora of molecular and physiological responses, including an adaptive response associated with gene activation. A primary step in hypoxia-mediated gene activation is the formation of the hypoxia-inducible factor (HIF-1) transcription factor complex. Hypoxia induces the stabilization of the HIF-1/spl alpha/, which in turn allows for the formation of the transcriptionally active protein complex. Up to date, the HIF-1-responsive genes that can modulate the PDT response have not been well identified. In the current study, we employed 5-aminolevulinic acid as a photosensitizer, 630 nm wavelength light-emitting diode (LED) manufactured by the Industrial Technology Research Institute as a light source. The experimental results demonstrated that cancer cells are more resistant to PDT under hypoxic status. PDT can transcriptionally induce or enhance HIF-1/spl alpha/ expression in different cervical cancer cell lines (SiHa, HeLa, Caski, C33A, HT-3), immortalized cervical epithelium cell line 183 A, and human umbilical vein endothelial cells (HUVECs). Pharmacological and genetic inhibition assays revealed that PI3K/Akt signaling critically involves in the activation of HIF-1/spl alpha/ by PDT in SiHa cells. When SiHa cells was treated with antisense HIF-1/spl alpha/ (20/spl mu/M), PDT activated HIF-1/spl alpha/ protein expression was markedly inhibited, and subsequently sensitized SiHa cells to PDT. Currently, pharmaceutical companies actively develop novel compounds targeting HIF-1/spl alpha/ as a promising cancer therapy. The results of this study will, therefore, provide important information to improve the therapeutic efficacy of PDT and have great clinical applicable potential.
{"title":"Hypoxia-inducible factor-1/spl alpha/ (HIF-1/spl alpha/) and photodynamic therapy","authors":"Lin-Hung Wei, C. Chou, J. Su","doi":"10.1109/APBP.2004.1412292","DOIUrl":"https://doi.org/10.1109/APBP.2004.1412292","url":null,"abstract":"Summary form only given. Photodynamic therapy (PDT) is a promising treatment modality that is being tested in the clinic for use in oncology. PDT requires three elements: light, a photosensitizer and oxygen. PDT-mediated oxidative stress elicits direct tumor cell damage and microvascular injury within exposed tumor. Microvasculature damage following PDT leads to a significant decrease in blood flow as well as severe and persistent tumor tissue hypoxia. Subsequently, tissue hypoxia can induce a plethora of molecular and physiological responses, including an adaptive response associated with gene activation. A primary step in hypoxia-mediated gene activation is the formation of the hypoxia-inducible factor (HIF-1) transcription factor complex. Hypoxia induces the stabilization of the HIF-1/spl alpha/, which in turn allows for the formation of the transcriptionally active protein complex. Up to date, the HIF-1-responsive genes that can modulate the PDT response have not been well identified. In the current study, we employed 5-aminolevulinic acid as a photosensitizer, 630 nm wavelength light-emitting diode (LED) manufactured by the Industrial Technology Research Institute as a light source. The experimental results demonstrated that cancer cells are more resistant to PDT under hypoxic status. PDT can transcriptionally induce or enhance HIF-1/spl alpha/ expression in different cervical cancer cell lines (SiHa, HeLa, Caski, C33A, HT-3), immortalized cervical epithelium cell line 183 A, and human umbilical vein endothelial cells (HUVECs). Pharmacological and genetic inhibition assays revealed that PI3K/Akt signaling critically involves in the activation of HIF-1/spl alpha/ by PDT in SiHa cells. When SiHa cells was treated with antisense HIF-1/spl alpha/ (20/spl mu/M), PDT activated HIF-1/spl alpha/ protein expression was markedly inhibited, and subsequently sensitized SiHa cells to PDT. Currently, pharmaceutical companies actively develop novel compounds targeting HIF-1/spl alpha/ as a promising cancer therapy. The results of this study will, therefore, provide important information to improve the therapeutic efficacy of PDT and have great clinical applicable potential.","PeriodicalId":346624,"journal":{"name":"The Second Asian and Pacific Rim Symposium on Biophotonics, 2004. APBP 2004.","volume":"42 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2004-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115819996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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