Pub Date : 2004-12-15DOI: 10.1109/APBP.2004.1412336
J.W. Huang, N.Z. Huang, D.L. Yang, S.N. Chen, Y.W. Chen, C. Lee
In the bio-medical mass-sampling system, an f-theta optical scanning system, using a rotating dodecagon mirror, can scan a range of angle on the medical samples test plate. The spot size of this optical system is about several micrometers and the scanning range is about 3 inch and the linearity is about 0.5 mm.
{"title":"A design of reflective F-/spl theta/ lens","authors":"J.W. Huang, N.Z. Huang, D.L. Yang, S.N. Chen, Y.W. Chen, C. Lee","doi":"10.1109/APBP.2004.1412336","DOIUrl":"https://doi.org/10.1109/APBP.2004.1412336","url":null,"abstract":"In the bio-medical mass-sampling system, an f-theta optical scanning system, using a rotating dodecagon mirror, can scan a range of angle on the medical samples test plate. The spot size of this optical system is about several micrometers and the scanning range is about 3 inch and the linearity is about 0.5 mm.","PeriodicalId":346624,"journal":{"name":"The Second Asian and Pacific Rim Symposium on Biophotonics, 2004. APBP 2004.","volume":"14 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2004-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127848813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
We retrospectively evaluated the safety, efficacy, predictability and the visual results of laser in situ keratomileusis using the B&L T-217 laser for the treatment of myopia and compound myopic astigmatism. It was proved to be predictable, safe and effective.
{"title":"Clinical experience with B&L T-217 excimer laser","authors":"Chao‐Chien Hu, Hsuei-Yuh Lu, Hung-Che Chen, Cheng-Kuo Cheng, Chun-Nan Chen","doi":"10.1109/APBP.2004.1412304","DOIUrl":"https://doi.org/10.1109/APBP.2004.1412304","url":null,"abstract":"We retrospectively evaluated the safety, efficacy, predictability and the visual results of laser in situ keratomileusis using the B&L T-217 laser for the treatment of myopia and compound myopic astigmatism. It was proved to be predictable, safe and effective.","PeriodicalId":346624,"journal":{"name":"The Second Asian and Pacific Rim Symposium on Biophotonics, 2004. APBP 2004.","volume":"55 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2004-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122436555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2004-12-15DOI: 10.1109/APBP.2004.1412297
Peng-Hsiang Weng, F. Kao
We are reporting implementation of high quality time resolved laser scanning microscopy through modulation techniques. Inexpensive and commercially available electronics and mechanical optical modulator were integrated with an externally triggered laser scanning microscope to form a phase sensitive lock-in detection imaging system. The unique mapping capability enabled allows time resolved imaging of luminescent samples and photo-sensitive devices.
{"title":"Modulation based time resolved laser scanning microscopy","authors":"Peng-Hsiang Weng, F. Kao","doi":"10.1109/APBP.2004.1412297","DOIUrl":"https://doi.org/10.1109/APBP.2004.1412297","url":null,"abstract":"We are reporting implementation of high quality time resolved laser scanning microscopy through modulation techniques. Inexpensive and commercially available electronics and mechanical optical modulator were integrated with an externally triggered laser scanning microscope to form a phase sensitive lock-in detection imaging system. The unique mapping capability enabled allows time resolved imaging of luminescent samples and photo-sensitive devices.","PeriodicalId":346624,"journal":{"name":"The Second Asian and Pacific Rim Symposium on Biophotonics, 2004. APBP 2004.","volume":"36 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2004-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128682685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2004-12-15DOI: 10.1109/APBP.2004.1412347
Chun-Chin Wang, Feng-Chieh Li, Wen Lo, C. Dong
Lung tumor is a major disease in Taiwan and the development of minimally invasive imaging to detect and diagnose lung tumor in vivo is of biomedical value. However, prior to in vivo investigation, it is important to obtain the multiphoton signatures associated with lung tumor ex vivo. In this investigation, we used multiphoton microscopy to image normal and cancerous lung tissues (adenocarcinoma). Our results show that a combination of harmonic generation and multiphoton autofluorescence imaging may be used to acquire structural information in discerning lung carcinoma from normal lung tissues.
{"title":"Optical biopsy of lung adenocarcinoma using multiphoton fluorescence and harmonic generation microscopy","authors":"Chun-Chin Wang, Feng-Chieh Li, Wen Lo, C. Dong","doi":"10.1109/APBP.2004.1412347","DOIUrl":"https://doi.org/10.1109/APBP.2004.1412347","url":null,"abstract":"Lung tumor is a major disease in Taiwan and the development of minimally invasive imaging to detect and diagnose lung tumor in vivo is of biomedical value. However, prior to in vivo investigation, it is important to obtain the multiphoton signatures associated with lung tumor ex vivo. In this investigation, we used multiphoton microscopy to image normal and cancerous lung tissues (adenocarcinoma). Our results show that a combination of harmonic generation and multiphoton autofluorescence imaging may be used to acquire structural information in discerning lung carcinoma from normal lung tissues.","PeriodicalId":346624,"journal":{"name":"The Second Asian and Pacific Rim Symposium on Biophotonics, 2004. APBP 2004.","volume":"7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2004-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130295397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2004-12-15DOI: 10.1109/APBP.2004.1412319
Chih-Wei Lu, M. Tsai, Yih-ming Wang, Y. Kiang, C.C. Yang
We demonstrate dynamic spectral shaping in an optical coherence tomography (OCT) system with multiple scans of segmented spectral bands and software signal processing. With an effective spectral full-width at half-maximum (FWHM) of 45 nm, centered at 950 nm, we compress the interference fringe FWHM from 31 microns down to 7 microns, which is smaller than the theoretical limit of 8.9 microns if a Gaussian spectral shape is assumed, through the combined effect of dispersion compensation and spectral shaping.
{"title":"Resolution improvement of optical coherence tomography with software spectral shaping","authors":"Chih-Wei Lu, M. Tsai, Yih-ming Wang, Y. Kiang, C.C. Yang","doi":"10.1109/APBP.2004.1412319","DOIUrl":"https://doi.org/10.1109/APBP.2004.1412319","url":null,"abstract":"We demonstrate dynamic spectral shaping in an optical coherence tomography (OCT) system with multiple scans of segmented spectral bands and software signal processing. With an effective spectral full-width at half-maximum (FWHM) of 45 nm, centered at 950 nm, we compress the interference fringe FWHM from 31 microns down to 7 microns, which is smaller than the theoretical limit of 8.9 microns if a Gaussian spectral shape is assumed, through the combined effect of dispersion compensation and spectral shaping.","PeriodicalId":346624,"journal":{"name":"The Second Asian and Pacific Rim Symposium on Biophotonics, 2004. APBP 2004.","volume":"77 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2004-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127640419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2004-12-15DOI: 10.1109/APBP.2004.1412327
Yiwen Ma, Kexin Xu, Y. Liu
Based on the principles of photoplethysmography and light absorption, this paper presents a preliminary research on noninvasive measurement of blood status of human body using optical method.
基于光容积脉搏波和光吸收原理，对光学法无创测量人体血液状态进行了初步研究。
{"title":"Preliminary research on feasibility of noninvasive sensing of blood multifunction using optical method","authors":"Yiwen Ma, Kexin Xu, Y. Liu","doi":"10.1109/APBP.2004.1412327","DOIUrl":"https://doi.org/10.1109/APBP.2004.1412327","url":null,"abstract":"Based on the principles of photoplethysmography and light absorption, this paper presents a preliminary research on noninvasive measurement of blood status of human body using optical method.","PeriodicalId":346624,"journal":{"name":"The Second Asian and Pacific Rim Symposium on Biophotonics, 2004. APBP 2004.","volume":"86 3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2004-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126290788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2004-12-15DOI: 10.1109/APBP.2004.1412325
Long‐Sheng Lu, Yen‐Bin Liu, Chau-Chung Wu, Chia-Wei Sun
Inflammation is a defense mechanism against various noxious stimuli. The recruitment of host leukocytes to sites of injury results in increased regional microvascular leakage and reactive oxygen species (ROS) generation. Excessive inflammatory activity not only eliminates offending stimuli but also result in tissue damage, as evidenced in reperfusion injury of the heart. To investigate spatial-temporal evolution of acute inflammation after myocardial reperfusion injury, we monitored microvascular leakage and reactive oxygen species generation with optical mapping technique. Reperfusion injury was performed on isolated blood-perfused rat heart, and it was labeled with dihydroethidium and large molecular weight tetramethylrhodamine conjugated dextran. Tissue was illuminated with a 532 nm laser, and epifluorescence at 580 and 650 nm was collected through 2 separate band pass filters. Our results indicate that 1. Optical mapping of myocardial inflammation is feasible; and 2. Reperfusion injury elicits substantial microvascular leakage and ROS production.
{"title":"Visualizing inflammatory activity in isolated perfused rat hearts","authors":"Long‐Sheng Lu, Yen‐Bin Liu, Chau-Chung Wu, Chia-Wei Sun","doi":"10.1109/APBP.2004.1412325","DOIUrl":"https://doi.org/10.1109/APBP.2004.1412325","url":null,"abstract":"Inflammation is a defense mechanism against various noxious stimuli. The recruitment of host leukocytes to sites of injury results in increased regional microvascular leakage and reactive oxygen species (ROS) generation. Excessive inflammatory activity not only eliminates offending stimuli but also result in tissue damage, as evidenced in reperfusion injury of the heart. To investigate spatial-temporal evolution of acute inflammation after myocardial reperfusion injury, we monitored microvascular leakage and reactive oxygen species generation with optical mapping technique. Reperfusion injury was performed on isolated blood-perfused rat heart, and it was labeled with dihydroethidium and large molecular weight tetramethylrhodamine conjugated dextran. Tissue was illuminated with a 532 nm laser, and epifluorescence at 580 and 650 nm was collected through 2 separate band pass filters. Our results indicate that 1. Optical mapping of myocardial inflammation is feasible; and 2. Reperfusion injury elicits substantial microvascular leakage and ROS production.","PeriodicalId":346624,"journal":{"name":"The Second Asian and Pacific Rim Symposium on Biophotonics, 2004. APBP 2004.","volume":"354 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2004-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126689443","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2004-12-15DOI: 10.1109/APBP.2004.1412374
L.V. Wang, X. Wang, G. Ku, X. Xie, G. Stoica
Based on the multiwavelength laser-based photoacoustic tomography, noninvasive imaging of cerebral blood oxygenation and blood volume in small-animal brains in vivo was realized. The high sensitivity of this technique is based on the spectroscopic differences between oxy- and deoxy-hemoglobins whereas its spatial resolution is diffraction-limited by the photoacoustic signals. The point-by-point distributions of hemoglobin oxygen saturation and total concentration of hemoglobin in the cerebral cortical venous vessels, altered by systemic physiological modulations including hyperoxia and hypoxia, were visualized successfully through the intact skin and skull. This technique can potentially accelerate the progress in neuroscience and provide important new insights into cerebrovascular physiology and brain function.
{"title":"High-resolution spectroscopic photoacoustic tomography for noninvasive functional imaging of small-animal brains in vivo","authors":"L.V. Wang, X. Wang, G. Ku, X. Xie, G. Stoica","doi":"10.1109/APBP.2004.1412374","DOIUrl":"https://doi.org/10.1109/APBP.2004.1412374","url":null,"abstract":"Based on the multiwavelength laser-based photoacoustic tomography, noninvasive imaging of cerebral blood oxygenation and blood volume in small-animal brains in vivo was realized. The high sensitivity of this technique is based on the spectroscopic differences between oxy- and deoxy-hemoglobins whereas its spatial resolution is diffraction-limited by the photoacoustic signals. The point-by-point distributions of hemoglobin oxygen saturation and total concentration of hemoglobin in the cerebral cortical venous vessels, altered by systemic physiological modulations including hyperoxia and hypoxia, were visualized successfully through the intact skin and skull. This technique can potentially accelerate the progress in neuroscience and provide important new insights into cerebrovascular physiology and brain function.","PeriodicalId":346624,"journal":{"name":"The Second Asian and Pacific Rim Symposium on Biophotonics, 2004. APBP 2004.","volume":"29 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2004-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128269677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A high-resolution second harmonic optical coherence tomography (SH-OCT) system is demonstrated using a spectrum broadened femtosecond Ti:sapphire laser. Images of highly organized collagen fibrils in the rat-tail tendon are demonstrated.
{"title":"High-resolution second harmonic optical coherence tomography","authors":"Yi Jiang, I. Tomov, Yimin Wang, Zhongping Chen","doi":"10.1117/12.591879","DOIUrl":"https://doi.org/10.1117/12.591879","url":null,"abstract":"A high-resolution second harmonic optical coherence tomography (SH-OCT) system is demonstrated using a spectrum broadened femtosecond Ti:sapphire laser. Images of highly organized collagen fibrils in the rat-tail tendon are demonstrated.","PeriodicalId":346624,"journal":{"name":"The Second Asian and Pacific Rim Symposium on Biophotonics, 2004. APBP 2004.","volume":"26 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2004-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131894665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2004-12-15DOI: 10.1109/APBP.2004.1412324
C.Y. Jhuang, J.Y. Chen, T.D. Cheng
The goal of this research is implementing the aspherical lens to design a scanning system. It is proposed to make the bio-optical system possess the higher resolution that spherical can't achieve.
{"title":"A design of scanning system based on the aspherical lens","authors":"C.Y. Jhuang, J.Y. Chen, T.D. Cheng","doi":"10.1109/APBP.2004.1412324","DOIUrl":"https://doi.org/10.1109/APBP.2004.1412324","url":null,"abstract":"The goal of this research is implementing the aspherical lens to design a scanning system. It is proposed to make the bio-optical system possess the higher resolution that spherical can't achieve.","PeriodicalId":346624,"journal":{"name":"The Second Asian and Pacific Rim Symposium on Biophotonics, 2004. APBP 2004.","volume":"33 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2004-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134110637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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