Pub Date : 2004-12-15DOI: 10.1109/APBP.2004.1412350
Chaug-Chun Lee, Cheng-hao Chien, Liang-Kung Wen, C. Dong
We developed a manipulation platform, which combines magnetic tweezers and the incubating system, with total internal reflection fluorescence microscopy (TIRFM). Our system can generate forces up to the nN range and such forces can be used to observe and characterize cell mechanical responses to external forces. Combined with TIRFM, cell surface stress-strain phenomena can also be investigated.
{"title":"A micromanipulation platform based on magnetic tweezers and total internal reflection fluorescence microscopy for cell mechanical studies","authors":"Chaug-Chun Lee, Cheng-hao Chien, Liang-Kung Wen, C. Dong","doi":"10.1109/APBP.2004.1412350","DOIUrl":"https://doi.org/10.1109/APBP.2004.1412350","url":null,"abstract":"We developed a manipulation platform, which combines magnetic tweezers and the incubating system, with total internal reflection fluorescence microscopy (TIRFM). Our system can generate forces up to the nN range and such forces can be used to observe and characterize cell mechanical responses to external forces. Combined with TIRFM, cell surface stress-strain phenomena can also be investigated.","PeriodicalId":346624,"journal":{"name":"The Second Asian and Pacific Rim Symposium on Biophotonics, 2004. APBP 2004.","volume":"122 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2004-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123477125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2004-12-15DOI: 10.1109/APBP.2004.1412290
Hsiao-Yin Lin, Chin-Tin Chen, Ching-Tsan Huang
Photodynamic inactivation of Staphylococcus aureus planktonic and biofilm cells by a photosensitizer, merocyanine 540 (MC 540) was investigated. For the planktonic experiments, the antimicrobial activity was enhanced with the increasing light dose, but an increase in light dose could not further improve the antimicrobial activity if the maximum excitation level attainable lay below the necessary minimum threshold level. A complete inactivation was achieved when the excitation level of MC 540 lay somewhere above the threshold level. The relationship between antimicrobial activity and the excitation level of MC 540 revealed that the more MC 540 was excited, the more S. aureus cells were killed. For the biofilm experiments, the antimicrobial activity was enhanced with the increasing light dose. No viable cells were detected when exposed to 15 /spl mu/g/ml MC 540 and a light dose of 600 J/cm/sup 2/ or 20 /spl mu/g/ml MC 540 and a light dose of 450 J/cm/sup 2/. The results from both planktonic and biofilm experiments suggest that the antimicrobial activity of photodynamic inactivation against S. aureus is closely related to the excitation level of MC 540.
{"title":"Merocyanine 540-mediated photodynamic therapy against Staphylococcus aureus planktonic and biofilm cells","authors":"Hsiao-Yin Lin, Chin-Tin Chen, Ching-Tsan Huang","doi":"10.1109/APBP.2004.1412290","DOIUrl":"https://doi.org/10.1109/APBP.2004.1412290","url":null,"abstract":"Photodynamic inactivation of Staphylococcus aureus planktonic and biofilm cells by a photosensitizer, merocyanine 540 (MC 540) was investigated. For the planktonic experiments, the antimicrobial activity was enhanced with the increasing light dose, but an increase in light dose could not further improve the antimicrobial activity if the maximum excitation level attainable lay below the necessary minimum threshold level. A complete inactivation was achieved when the excitation level of MC 540 lay somewhere above the threshold level. The relationship between antimicrobial activity and the excitation level of MC 540 revealed that the more MC 540 was excited, the more S. aureus cells were killed. For the biofilm experiments, the antimicrobial activity was enhanced with the increasing light dose. No viable cells were detected when exposed to 15 /spl mu/g/ml MC 540 and a light dose of 600 J/cm/sup 2/ or 20 /spl mu/g/ml MC 540 and a light dose of 450 J/cm/sup 2/. The results from both planktonic and biofilm experiments suggest that the antimicrobial activity of photodynamic inactivation against S. aureus is closely related to the excitation level of MC 540.","PeriodicalId":346624,"journal":{"name":"The Second Asian and Pacific Rim Symposium on Biophotonics, 2004. APBP 2004.","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2004-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126227666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2004-12-15DOI: 10.1109/APBP.2004.1412315
Y. Watanabe, Y. Hayasaka, M. Sato
We demonstrated full-field optical coherence tomography (OCT) using achromatic phase shifting with a rotating polarizer. Using this phase shifter and conventional phase shift method, we obtained en-face OCT images of an onion at different depths.
{"title":"Full-field optical coherence tomography with achromatic phase shifter","authors":"Y. Watanabe, Y. Hayasaka, M. Sato","doi":"10.1109/APBP.2004.1412315","DOIUrl":"https://doi.org/10.1109/APBP.2004.1412315","url":null,"abstract":"We demonstrated full-field optical coherence tomography (OCT) using achromatic phase shifting with a rotating polarizer. Using this phase shifter and conventional phase shift method, we obtained en-face OCT images of an onion at different depths.","PeriodicalId":346624,"journal":{"name":"The Second Asian and Pacific Rim Symposium on Biophotonics, 2004. APBP 2004.","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2004-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131079383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2004-12-15DOI: 10.1109/APBP.2004.1412309
T. Iwai
We have so far concerned with developing diffuse reflectometry for static and dynamic light back-scattered multiply from extremely dense media to characterizing scatters in size, shape, the state of aggregation, and the absorption. In this report, we will introduce our researches on applications of multiple backscattering phenomena to new diffuse reflectometry.
{"title":"Spatiotemporal diffuse reflectometry","authors":"T. Iwai","doi":"10.1109/APBP.2004.1412309","DOIUrl":"https://doi.org/10.1109/APBP.2004.1412309","url":null,"abstract":"We have so far concerned with developing diffuse reflectometry for static and dynamic light back-scattered multiply from extremely dense media to characterizing scatters in size, shape, the state of aggregation, and the absorption. In this report, we will introduce our researches on applications of multiple backscattering phenomena to new diffuse reflectometry.","PeriodicalId":346624,"journal":{"name":"The Second Asian and Pacific Rim Symposium on Biophotonics, 2004. APBP 2004.","volume":"22 1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2004-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123102635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2004-12-15DOI: 10.1109/APBP.2004.1412343
Feng-Chieh Li, Chun-Chin Wang, Sung-Jan Lin, S. Jee, C. Dong
For many years, the dorsal skinfold chamber has been used for intravital observation in animal models. We have developed a chamber built using medical-grade titanium. Our design allows long-term, high resolution, and large-area observation in nude mice in vivo. Combined with two-photon fluorescence and second-harmonic generation microscopy, our chamber can be used to obtain qualitative and quantitative information for in vivo analysis of diseases such as tumors.
{"title":"Dorsal skinfold titanium chamber for noninvasive imaging in nude mice using multiphoton and harmonic generation microscopy","authors":"Feng-Chieh Li, Chun-Chin Wang, Sung-Jan Lin, S. Jee, C. Dong","doi":"10.1109/APBP.2004.1412343","DOIUrl":"https://doi.org/10.1109/APBP.2004.1412343","url":null,"abstract":"For many years, the dorsal skinfold chamber has been used for intravital observation in animal models. We have developed a chamber built using medical-grade titanium. Our design allows long-term, high resolution, and large-area observation in nude mice in vivo. Combined with two-photon fluorescence and second-harmonic generation microscopy, our chamber can be used to obtain qualitative and quantitative information for in vivo analysis of diseases such as tumors.","PeriodicalId":346624,"journal":{"name":"The Second Asian and Pacific Rim Symposium on Biophotonics, 2004. APBP 2004.","volume":"53 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2004-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127717486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2004-12-15DOI: 10.1109/APBP.2004.1412342
S. Teng, Ju-Li Peng, H. Lin, Hai-Yin Wu, Wen Lo, Yen Sun, Wei-Chou Lin, Sung-Jan Lin, S. Jee, H. Tan, C. Dong
This purpose of this study is to demonstrate the feasibility of using multiphoton microscopy in imaging eye surface. Specifically, the cornea, limbus, conjunctiva, and sclera were imaged using multiphoton induced fluorescence and second-harmonic generation (SHG) imaging.
{"title":"Multiphoton imaging of porcine eye: cornea, limbus, conjunctiva, and sclera","authors":"S. Teng, Ju-Li Peng, H. Lin, Hai-Yin Wu, Wen Lo, Yen Sun, Wei-Chou Lin, Sung-Jan Lin, S. Jee, H. Tan, C. Dong","doi":"10.1109/APBP.2004.1412342","DOIUrl":"https://doi.org/10.1109/APBP.2004.1412342","url":null,"abstract":"This purpose of this study is to demonstrate the feasibility of using multiphoton microscopy in imaging eye surface. Specifically, the cornea, limbus, conjunctiva, and sclera were imaged using multiphoton induced fluorescence and second-harmonic generation (SHG) imaging.","PeriodicalId":346624,"journal":{"name":"The Second Asian and Pacific Rim Symposium on Biophotonics, 2004. APBP 2004.","volume":"6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2004-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133725488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2004-12-15DOI: 10.1109/APBP.2004.1412333
Sheng-Hong Yu, Cheng-Hau Chen, H. Chiang
An evanescent wave fluorescence-based fiber-optic biosensor is developed for high sensitivity, low cost, compact design and real-time in-situ monitoring. In the research, we accomplished the fiber-optical biosensor system to monitor the reaction of IgG-Anti rabbit IgG and Biotin-Streptavidin, and the kinetic association and dissociation constant of target analytes were calculated.
研制了一种高灵敏度、低成本、结构紧凑、实时监测的荧光倏逝波光纤生物传感器。在本研究中，我们完成了监测IgG- anti rabbit IgG与Biotin-Streptavidin反应的光纤生物传感器系统，并计算了目标分析物的动力学关联常数和解离常数。
{"title":"Fiber optic evanescent wave biosensor for biomolecular detection","authors":"Sheng-Hong Yu, Cheng-Hau Chen, H. Chiang","doi":"10.1109/APBP.2004.1412333","DOIUrl":"https://doi.org/10.1109/APBP.2004.1412333","url":null,"abstract":"An evanescent wave fluorescence-based fiber-optic biosensor is developed for high sensitivity, low cost, compact design and real-time in-situ monitoring. In the research, we accomplished the fiber-optical biosensor system to monitor the reaction of IgG-Anti rabbit IgG and Biotin-Streptavidin, and the kinetic association and dissociation constant of target analytes were calculated.","PeriodicalId":346624,"journal":{"name":"The Second Asian and Pacific Rim Symposium on Biophotonics, 2004. APBP 2004.","volume":"1996 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2004-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123785120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2004-12-15DOI: 10.1109/APBP.2004.1412269
Y. Mashita, M. Ueno, M. Ohmi, H. Arimoto, N. Kakuta, M. Haruna, Y. Yamada
We have measured the scattering and the absorption coefficients of nails at wavelengths from 800 to 1900 nm by the combination of integrating sphere measurements and an inverse Monte Carlo method. Also the dependences of these coefficients on surface roughness and humidity were investigated.
{"title":"Measurement of the optical properties of nails","authors":"Y. Mashita, M. Ueno, M. Ohmi, H. Arimoto, N. Kakuta, M. Haruna, Y. Yamada","doi":"10.1109/APBP.2004.1412269","DOIUrl":"https://doi.org/10.1109/APBP.2004.1412269","url":null,"abstract":"We have measured the scattering and the absorption coefficients of nails at wavelengths from 800 to 1900 nm by the combination of integrating sphere measurements and an inverse Monte Carlo method. Also the dependences of these coefficients on surface roughness and humidity were investigated.","PeriodicalId":346624,"journal":{"name":"The Second Asian and Pacific Rim Symposium on Biophotonics, 2004. APBP 2004.","volume":"44 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2004-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122430628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2004-12-15DOI: 10.1109/APBP.2004.1412272
A. Uchibori, T. Kojima, H. Tsunashima, T. Shiozawa
We conducted a near-infrared spectroscopic experiment of brain activity for language processing. Syntactic aspects of language are particularly focused on in the stimuli. Increase in blood flow around Broca 's area is measured, as expected.
{"title":"Near-infrared spectroscopic measurement of brain activity for syntactic processing","authors":"A. Uchibori, T. Kojima, H. Tsunashima, T. Shiozawa","doi":"10.1109/APBP.2004.1412272","DOIUrl":"https://doi.org/10.1109/APBP.2004.1412272","url":null,"abstract":"We conducted a near-infrared spectroscopic experiment of brain activity for language processing. Syntactic aspects of language are particularly focused on in the stimuli. Increase in blood flow around Broca 's area is measured, as expected.","PeriodicalId":346624,"journal":{"name":"The Second Asian and Pacific Rim Symposium on Biophotonics, 2004. APBP 2004.","volume":"44 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2004-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116198805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2004-12-15DOI: 10.1109/APBP.2004.1412375
Y. Kunihiro, K. Kagei, M. Fujii, K. Nakayama
We present a phantom study of NIR tomographic imaging based on the pulsed-ultrasound modulation of diffuse light in turbid media. The bottleneck of the method is the very low S/N which cannot be overcome by the conventional signal-averaging because of the slowly-varying light phase on the detector. We proposed a simple model for the detected signal and validated it experimentally. Furthermore, we proposed the frequency-doubling-and-averaging method to recover the signal, and demonstrated its feasibility through the phantom experiment.
{"title":"Experimental study of pulsed ultrasono-optical tomography","authors":"Y. Kunihiro, K. Kagei, M. Fujii, K. Nakayama","doi":"10.1109/APBP.2004.1412375","DOIUrl":"https://doi.org/10.1109/APBP.2004.1412375","url":null,"abstract":"We present a phantom study of NIR tomographic imaging based on the pulsed-ultrasound modulation of diffuse light in turbid media. The bottleneck of the method is the very low S/N which cannot be overcome by the conventional signal-averaging because of the slowly-varying light phase on the detector. We proposed a simple model for the detected signal and validated it experimentally. Furthermore, we proposed the frequency-doubling-and-averaging method to recover the signal, and demonstrated its feasibility through the phantom experiment.","PeriodicalId":346624,"journal":{"name":"The Second Asian and Pacific Rim Symposium on Biophotonics, 2004. APBP 2004.","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2004-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114698079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：