Pub Date : 2004-12-15DOI: 10.1109/APBP.2004.1412370
Yen-Sheng Lu, Yuan-Peng Huang, J. Yeh, Chengkuo Lee
We demonstrated a novel approach to trigger the dielectrophoretic force to manipulate biological cells via projected flash images. The attractive and repulsive force regions are formed due to effectively initiated dielectrophoretic force, where these regions are the bright and dark area among a projected image on a liquid reservoir depending on the polarization states. In present study, the attractive force draw in live cells, saccharomyces cerevisiae, and live cells can be manipulated inside reservoir without any microchannel confinement in a programmable manner. The relation between distance from the boundary and average velocity is also discussed.
{"title":"Image driven cell manipulation using optical dielectrophoresis (ODEP)","authors":"Yen-Sheng Lu, Yuan-Peng Huang, J. Yeh, Chengkuo Lee","doi":"10.1109/APBP.2004.1412370","DOIUrl":"https://doi.org/10.1109/APBP.2004.1412370","url":null,"abstract":"We demonstrated a novel approach to trigger the dielectrophoretic force to manipulate biological cells via projected flash images. The attractive and repulsive force regions are formed due to effectively initiated dielectrophoretic force, where these regions are the bright and dark area among a projected image on a liquid reservoir depending on the polarization states. In present study, the attractive force draw in live cells, saccharomyces cerevisiae, and live cells can be manipulated inside reservoir without any microchannel confinement in a programmable manner. The relation between distance from the boundary and average velocity is also discussed.","PeriodicalId":346624,"journal":{"name":"The Second Asian and Pacific Rim Symposium on Biophotonics, 2004. APBP 2004.","volume":"198 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2004-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115491634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2004-12-15DOI: 10.1109/APBP.2004.1412267
S. Wang, Lanqing Xu, Hui Li, Y. Yamada
Monte Carlo method was used to simulate the propagation behavior of 2-D Stokes vectors and Mueller matrices under various conditions. The effect of different incident polarization states, tissue optical parameters and Fresnel matrices at the boundary are fully considered.
{"title":"Diffusely scattered polarized light in turbid media: Monte Carlo Simulation","authors":"S. Wang, Lanqing Xu, Hui Li, Y. Yamada","doi":"10.1109/APBP.2004.1412267","DOIUrl":"https://doi.org/10.1109/APBP.2004.1412267","url":null,"abstract":"Monte Carlo method was used to simulate the propagation behavior of 2-D Stokes vectors and Mueller matrices under various conditions. The effect of different incident polarization states, tissue optical parameters and Fresnel matrices at the boundary are fully considered.","PeriodicalId":346624,"journal":{"name":"The Second Asian and Pacific Rim Symposium on Biophotonics, 2004. APBP 2004.","volume":"203 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2004-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123967667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2004-12-15DOI: 10.1109/APBP.2004.1412287
Yuan Liu, Hsuan-Shu Lee, Hsiao-Ching Chen, L. Chiou, Guan-Tarn Huang, Wen Lo, C. Dong
In this work, we apply multiphoton microscopy in diagnosing toxin (CCl/sub 4/) induced liver fibrosis in mice. We found that multiphoton induced spectroscopic signatures may be used to identify stellate cell proliferation and necrotic sites. Our study suggests that multiphoton imaging may be developed into an effective diagnosis tool for liver fibrosis in vivo.
{"title":"Optical biopsy of liver fibrosis using multiphoton microscopy","authors":"Yuan Liu, Hsuan-Shu Lee, Hsiao-Ching Chen, L. Chiou, Guan-Tarn Huang, Wen Lo, C. Dong","doi":"10.1109/APBP.2004.1412287","DOIUrl":"https://doi.org/10.1109/APBP.2004.1412287","url":null,"abstract":"In this work, we apply multiphoton microscopy in diagnosing toxin (CCl/sub 4/) induced liver fibrosis in mice. We found that multiphoton induced spectroscopic signatures may be used to identify stellate cell proliferation and necrotic sites. Our study suggests that multiphoton imaging may be developed into an effective diagnosis tool for liver fibrosis in vivo.","PeriodicalId":346624,"journal":{"name":"The Second Asian and Pacific Rim Symposium on Biophotonics, 2004. APBP 2004.","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2004-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130261820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2004-12-15DOI: 10.1109/APBP.2004.1412271
S. Fukushima, T. Araki, Y. Sakaguchi, Y. Tohno
Calcium content in vascular wall at arterial bifurcation was measured with atomic emission spectrometry, and blood flow in the bifurcation was simulated by computational fluid dynamics. The results suggested hemodynamic factors affect vascular wall calcification.
{"title":"Relationship between calcium content and hemodynamics at arterial bifurcation","authors":"S. Fukushima, T. Araki, Y. Sakaguchi, Y. Tohno","doi":"10.1109/APBP.2004.1412271","DOIUrl":"https://doi.org/10.1109/APBP.2004.1412271","url":null,"abstract":"Calcium content in vascular wall at arterial bifurcation was measured with atomic emission spectrometry, and blood flow in the bifurcation was simulated by computational fluid dynamics. The results suggested hemodynamic factors affect vascular wall calcification.","PeriodicalId":346624,"journal":{"name":"The Second Asian and Pacific Rim Symposium on Biophotonics, 2004. APBP 2004.","volume":"25 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2004-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128635639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2004-12-15DOI: 10.1109/APBP.2004.1412292
Lin-Hung Wei, C. Chou, J. Su
Summary form only given. Photodynamic therapy (PDT) is a promising treatment modality that is being tested in the clinic for use in oncology. PDT requires three elements: light, a photosensitizer and oxygen. PDT-mediated oxidative stress elicits direct tumor cell damage and microvascular injury within exposed tumor. Microvasculature damage following PDT leads to a significant decrease in blood flow as well as severe and persistent tumor tissue hypoxia. Subsequently, tissue hypoxia can induce a plethora of molecular and physiological responses, including an adaptive response associated with gene activation. A primary step in hypoxia-mediated gene activation is the formation of the hypoxia-inducible factor (HIF-1) transcription factor complex. Hypoxia induces the stabilization of the HIF-1/spl alpha/, which in turn allows for the formation of the transcriptionally active protein complex. Up to date, the HIF-1-responsive genes that can modulate the PDT response have not been well identified. In the current study, we employed 5-aminolevulinic acid as a photosensitizer, 630 nm wavelength light-emitting diode (LED) manufactured by the Industrial Technology Research Institute as a light source. The experimental results demonstrated that cancer cells are more resistant to PDT under hypoxic status. PDT can transcriptionally induce or enhance HIF-1/spl alpha/ expression in different cervical cancer cell lines (SiHa, HeLa, Caski, C33A, HT-3), immortalized cervical epithelium cell line 183 A, and human umbilical vein endothelial cells (HUVECs). Pharmacological and genetic inhibition assays revealed that PI3K/Akt signaling critically involves in the activation of HIF-1/spl alpha/ by PDT in SiHa cells. When SiHa cells was treated with antisense HIF-1/spl alpha/ (20/spl mu/M), PDT activated HIF-1/spl alpha/ protein expression was markedly inhibited, and subsequently sensitized SiHa cells to PDT. Currently, pharmaceutical companies actively develop novel compounds targeting HIF-1/spl alpha/ as a promising cancer therapy. The results of this study will, therefore, provide important information to improve the therapeutic efficacy of PDT and have great clinical applicable potential.
{"title":"Hypoxia-inducible factor-1/spl alpha/ (HIF-1/spl alpha/) and photodynamic therapy","authors":"Lin-Hung Wei, C. Chou, J. Su","doi":"10.1109/APBP.2004.1412292","DOIUrl":"https://doi.org/10.1109/APBP.2004.1412292","url":null,"abstract":"Summary form only given. Photodynamic therapy (PDT) is a promising treatment modality that is being tested in the clinic for use in oncology. PDT requires three elements: light, a photosensitizer and oxygen. PDT-mediated oxidative stress elicits direct tumor cell damage and microvascular injury within exposed tumor. Microvasculature damage following PDT leads to a significant decrease in blood flow as well as severe and persistent tumor tissue hypoxia. Subsequently, tissue hypoxia can induce a plethora of molecular and physiological responses, including an adaptive response associated with gene activation. A primary step in hypoxia-mediated gene activation is the formation of the hypoxia-inducible factor (HIF-1) transcription factor complex. Hypoxia induces the stabilization of the HIF-1/spl alpha/, which in turn allows for the formation of the transcriptionally active protein complex. Up to date, the HIF-1-responsive genes that can modulate the PDT response have not been well identified. In the current study, we employed 5-aminolevulinic acid as a photosensitizer, 630 nm wavelength light-emitting diode (LED) manufactured by the Industrial Technology Research Institute as a light source. The experimental results demonstrated that cancer cells are more resistant to PDT under hypoxic status. PDT can transcriptionally induce or enhance HIF-1/spl alpha/ expression in different cervical cancer cell lines (SiHa, HeLa, Caski, C33A, HT-3), immortalized cervical epithelium cell line 183 A, and human umbilical vein endothelial cells (HUVECs). Pharmacological and genetic inhibition assays revealed that PI3K/Akt signaling critically involves in the activation of HIF-1/spl alpha/ by PDT in SiHa cells. When SiHa cells was treated with antisense HIF-1/spl alpha/ (20/spl mu/M), PDT activated HIF-1/spl alpha/ protein expression was markedly inhibited, and subsequently sensitized SiHa cells to PDT. Currently, pharmaceutical companies actively develop novel compounds targeting HIF-1/spl alpha/ as a promising cancer therapy. The results of this study will, therefore, provide important information to improve the therapeutic efficacy of PDT and have great clinical applicable potential.","PeriodicalId":346624,"journal":{"name":"The Second Asian and Pacific Rim Symposium on Biophotonics, 2004. APBP 2004.","volume":"42 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2004-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115819996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2004-12-15DOI: 10.1109/APBP.2004.1412335
Chia-Wei Sun, Cheng-Kuang Lee, Hsiang-Chieh Lee, Po-Lei Lee, C.C. Yang, Cho-Pei Jiang, Tsz-Yi Yang, Y. Tong, J. Hsieh
We demonstrate the utility of near-infrared spectroscopy in monitoring of the human visual cortex. The typical hemodynamic signals expected are observed in our preliminary experiments.
我们展示了近红外光谱在监测人类视觉皮层中的效用。我们在初步实验中观察到预期的典型血流动力学信号。
{"title":"Near-infrared spectroscopy studies of visual evoked responses during photon stimulation","authors":"Chia-Wei Sun, Cheng-Kuang Lee, Hsiang-Chieh Lee, Po-Lei Lee, C.C. Yang, Cho-Pei Jiang, Tsz-Yi Yang, Y. Tong, J. Hsieh","doi":"10.1109/APBP.2004.1412335","DOIUrl":"https://doi.org/10.1109/APBP.2004.1412335","url":null,"abstract":"We demonstrate the utility of near-infrared spectroscopy in monitoring of the human visual cortex. The typical hemodynamic signals expected are observed in our preliminary experiments.","PeriodicalId":346624,"journal":{"name":"The Second Asian and Pacific Rim Symposium on Biophotonics, 2004. APBP 2004.","volume":"111 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2004-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121156915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2004-12-15DOI: 10.1109/APBP.2004.1412274
C. Wen, K. Yuan, P.T. Chou, C. Lee, J.W. Huang
According to the development of bio-technology, the requirements of metrology in biochip measuring become more precise and noncontact measurement like optical detector or ultra wave sensor is plenty used in this field. Laser scanning technology is used in examine biochip for its characteristic of noncontact measurement, high performance and good sensitivities. This paper emphasis on the lens designed for micro meter focusing scanning for bio-medical systems. The scanner exploits the functional advantages and the optical system has small spot size, great linearity and large depth of focus. The optical design of the scanning lenses is discussed and the scanner fabrication is introduced.
{"title":"A design f-theta lens for bio-medical system","authors":"C. Wen, K. Yuan, P.T. Chou, C. Lee, J.W. Huang","doi":"10.1109/APBP.2004.1412274","DOIUrl":"https://doi.org/10.1109/APBP.2004.1412274","url":null,"abstract":"According to the development of bio-technology, the requirements of metrology in biochip measuring become more precise and noncontact measurement like optical detector or ultra wave sensor is plenty used in this field. Laser scanning technology is used in examine biochip for its characteristic of noncontact measurement, high performance and good sensitivities. This paper emphasis on the lens designed for micro meter focusing scanning for bio-medical systems. The scanner exploits the functional advantages and the optical system has small spot size, great linearity and large depth of focus. The optical design of the scanning lenses is discussed and the scanner fabrication is introduced.","PeriodicalId":346624,"journal":{"name":"The Second Asian and Pacific Rim Symposium on Biophotonics, 2004. APBP 2004.","volume":"42 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2004-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126795460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2004-12-15DOI: 10.1109/APBP.2004.1412307
Hsin-Cheng Liu, W. Lan
The aim of this study was to evaluate the effectiveness of diode laser combined with glass ionomer cement in the treatment of cervical dentin abrasion hypersensitivity.
本研究的目的是评价二极管激光联合玻璃离聚体骨水泥治疗颈牙本质磨损超敏反应的有效性。
{"title":"Treatment of cervical dentin abrasion hypersensitivity by diode laser and glass ionomer cement","authors":"Hsin-Cheng Liu, W. Lan","doi":"10.1109/APBP.2004.1412307","DOIUrl":"https://doi.org/10.1109/APBP.2004.1412307","url":null,"abstract":"The aim of this study was to evaluate the effectiveness of diode laser combined with glass ionomer cement in the treatment of cervical dentin abrasion hypersensitivity.","PeriodicalId":346624,"journal":{"name":"The Second Asian and Pacific Rim Symposium on Biophotonics, 2004. APBP 2004.","volume":"29 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2004-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127648429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2004-12-15DOI: 10.1109/APBP.2004.1412372
Liang Yu Yao, Chien-Chih Huang, K. Weng, Chien-Cheng Yang, Chia-Wei Sun, M. Tsai, C.C. Yang
To miniaturize the system, we use an LED and a pin photodetector for light-induced fluorescence detection of capillary electrophoreses. To overcome the drawback of low gain in a photodetector, we use the technique of lock-in amplification.
{"title":"A microcapillary electrophoreses fluorescence detection system with a blue-light-emitting diode as light source","authors":"Liang Yu Yao, Chien-Chih Huang, K. Weng, Chien-Cheng Yang, Chia-Wei Sun, M. Tsai, C.C. Yang","doi":"10.1109/APBP.2004.1412372","DOIUrl":"https://doi.org/10.1109/APBP.2004.1412372","url":null,"abstract":"To miniaturize the system, we use an LED and a pin photodetector for light-induced fluorescence detection of capillary electrophoreses. To overcome the drawback of low gain in a photodetector, we use the technique of lock-in amplification.","PeriodicalId":346624,"journal":{"name":"The Second Asian and Pacific Rim Symposium on Biophotonics, 2004. APBP 2004.","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2004-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130810456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2004-12-15DOI: 10.1109/APBP.2004.1412248
S. Bown
Photodynamic therapy (PDT) has evolved recently by the availability of lasers and optical fibres suitable for light delivery to many sites within the body, and synthesis of more effective photosensitizers. From a clinical point of view, PDT is a way of producing localised tissue destruction with light. One of the most striking aspects of PDT induced tissue destruction is that connective tissues like collagen and elastin are largely unaffected. PDT offers many challenges and opportunities for existing and new optical technologies, including lasers. The basic principle can be applied to disease processes in a wide range of organs. The secret to making the most of this evolving new therapeutic modality is to understand the biology of what PDT can do to living tissue, identify when that is relevant to the treatment of human disease and then ensure that adequate drug and light doses are delivered to all relevant sites.
{"title":"Scientists and clinicians create a bright future for photodynamic therapy (PDT)","authors":"S. Bown","doi":"10.1109/APBP.2004.1412248","DOIUrl":"https://doi.org/10.1109/APBP.2004.1412248","url":null,"abstract":"Photodynamic therapy (PDT) has evolved recently by the availability of lasers and optical fibres suitable for light delivery to many sites within the body, and synthesis of more effective photosensitizers. From a clinical point of view, PDT is a way of producing localised tissue destruction with light. One of the most striking aspects of PDT induced tissue destruction is that connective tissues like collagen and elastin are largely unaffected. PDT offers many challenges and opportunities for existing and new optical technologies, including lasers. The basic principle can be applied to disease processes in a wide range of organs. The secret to making the most of this evolving new therapeutic modality is to understand the biology of what PDT can do to living tissue, identify when that is relevant to the treatment of human disease and then ensure that adequate drug and light doses are delivered to all relevant sites.","PeriodicalId":346624,"journal":{"name":"The Second Asian and Pacific Rim Symposium on Biophotonics, 2004. APBP 2004.","volume":"61 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2004-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116990340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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