Pub Date : 2004-12-15DOI: 10.1109/APBP.2004.1412299
Zhiwei Huang, H. Lui, D. McLean, M. Korbelik, H. Zeng
This study evaluated and compared the diagnostic ability of near-infrared (NIR) autofluorescence spectroscopy, NIR Raman spectroscopy, and the superimposed Raman and NIR autofluorescence spectroscopy for in vivo detection of malignant tumors using a murine model.
{"title":"Combining Raman spectroscopy with background near-infrared autofluorescence to improve the noninvasive detection of malignant tumors","authors":"Zhiwei Huang, H. Lui, D. McLean, M. Korbelik, H. Zeng","doi":"10.1109/APBP.2004.1412299","DOIUrl":"https://doi.org/10.1109/APBP.2004.1412299","url":null,"abstract":"This study evaluated and compared the diagnostic ability of near-infrared (NIR) autofluorescence spectroscopy, NIR Raman spectroscopy, and the superimposed Raman and NIR autofluorescence spectroscopy for in vivo detection of malignant tumors using a murine model.","PeriodicalId":346624,"journal":{"name":"The Second Asian and Pacific Rim Symposium on Biophotonics, 2004. APBP 2004.","volume":"354 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2004-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132554384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2004-12-15DOI: 10.1109/APBP.2004.1412284
M. Gu, X. Gan
In this paper, we demonstrate that the penetration depth under two-photon excitation is limited by the strength of two-photon fluorescence and is not necessarily larger than that under single-photon excitation, although image resolution is much higher in the former case.
{"title":"Penetration depth in multiphoton fluorescence microscopy","authors":"M. Gu, X. Gan","doi":"10.1109/APBP.2004.1412284","DOIUrl":"https://doi.org/10.1109/APBP.2004.1412284","url":null,"abstract":"In this paper, we demonstrate that the penetration depth under two-photon excitation is limited by the strength of two-photon fluorescence and is not necessarily larger than that under single-photon excitation, although image resolution is much higher in the former case.","PeriodicalId":346624,"journal":{"name":"The Second Asian and Pacific Rim Symposium on Biophotonics, 2004. APBP 2004.","volume":"199 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2004-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122555206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2004-12-15DOI: 10.1109/APBP.2004.1412346
Tzu-Yu Lin, Sung-Jan Lin, Hsiao-Ching Chen, Hsuan-Shu Lee, S. Teng, Wen Lo, C. Dong
The use of seeding scaffolds has facilitated advances in tissue engineering. We found that multiphoton microscopy is useful in imaging commonly used tissue engineering scaffolds such as polyglycolic acid (PGA).
{"title":"Multiphoton characterization of tissue engineering scaffolds","authors":"Tzu-Yu Lin, Sung-Jan Lin, Hsiao-Ching Chen, Hsuan-Shu Lee, S. Teng, Wen Lo, C. Dong","doi":"10.1109/APBP.2004.1412346","DOIUrl":"https://doi.org/10.1109/APBP.2004.1412346","url":null,"abstract":"The use of seeding scaffolds has facilitated advances in tissue engineering. We found that multiphoton microscopy is useful in imaging commonly used tissue engineering scaffolds such as polyglycolic acid (PGA).","PeriodicalId":346624,"journal":{"name":"The Second Asian and Pacific Rim Symposium on Biophotonics, 2004. APBP 2004.","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2004-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128947524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2004-12-15DOI: 10.1109/APBP.2004.1412313
G. Nishimura, M. Tamura
The time-of-flight (TOF) method and the diffuse reflectance (DR) method were applied for the characterization of optical parameters in living tissue in the region of 1-2 /spl mu/m. The absorption coefficient obtained by the TOF method was consistent with the content of water in the tissue. The DR measurement indicates the very large attenuation in the wavelength region above 1.4 /spl mu/m. In this region, it is suggested that the optical transport does not diffuse well and it is localized at the light source.
{"title":"Characterization of tissue optical parameters in the region of 1-2 /spl mu/m","authors":"G. Nishimura, M. Tamura","doi":"10.1109/APBP.2004.1412313","DOIUrl":"https://doi.org/10.1109/APBP.2004.1412313","url":null,"abstract":"The time-of-flight (TOF) method and the diffuse reflectance (DR) method were applied for the characterization of optical parameters in living tissue in the region of 1-2 /spl mu/m. The absorption coefficient obtained by the TOF method was consistent with the content of water in the tissue. The DR measurement indicates the very large attenuation in the wavelength region above 1.4 /spl mu/m. In this region, it is suggested that the optical transport does not diffuse well and it is localized at the light source.","PeriodicalId":346624,"journal":{"name":"The Second Asian and Pacific Rim Symposium on Biophotonics, 2004. APBP 2004.","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2004-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128448614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2004-12-15DOI: 10.1109/APBP.2004.1412300
M. Ogura, M. Obara, S. Sato, H. Ashida
We demonstrated in vivo targeted gene delivery to rat skin by direct irradiation with nanosecond laser pulses. Expressions of enhanced green fluorescent protein (EGFP) were observed only in the area irradiated with laser pulses.
{"title":"In vivo targeted gene delivery to skin by direct irradiation with nanosecond laser pulses","authors":"M. Ogura, M. Obara, S. Sato, H. Ashida","doi":"10.1109/APBP.2004.1412300","DOIUrl":"https://doi.org/10.1109/APBP.2004.1412300","url":null,"abstract":"We demonstrated in vivo targeted gene delivery to rat skin by direct irradiation with nanosecond laser pulses. Expressions of enhanced green fluorescent protein (EGFP) were observed only in the area irradiated with laser pulses.","PeriodicalId":346624,"journal":{"name":"The Second Asian and Pacific Rim Symposium on Biophotonics, 2004. APBP 2004.","volume":"22 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2004-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116425449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2004-12-15DOI: 10.1109/APBP.2004.1412268
D. Li
This paper mainly describes the database design for an accurate, reliable and clinical technique to wound management. The wound size assessment should include length, width, area and perimeter. The program enables the user to calculate the linear distance (width and length) of an image. The mouse is used to trace the cursor around the image outline (perimeter). Manual calibration of the measurement can be carried out to increase wound assessment accuracy and reliability. In addition, at any time, a patient's wound images and image data may be viewed in chronological order for the purpose of comparison. This provides a visual and informative record of changes to the wound over time. Finally, wound measurement software provides all the features necessary to work with databases, such as entering new data, and modifying or viewing existing data.
{"title":"Database design and implementation for wound measurement system","authors":"D. Li","doi":"10.1109/APBP.2004.1412268","DOIUrl":"https://doi.org/10.1109/APBP.2004.1412268","url":null,"abstract":"This paper mainly describes the database design for an accurate, reliable and clinical technique to wound management. The wound size assessment should include length, width, area and perimeter. The program enables the user to calculate the linear distance (width and length) of an image. The mouse is used to trace the cursor around the image outline (perimeter). Manual calibration of the measurement can be carried out to increase wound assessment accuracy and reliability. In addition, at any time, a patient's wound images and image data may be viewed in chronological order for the purpose of comparison. This provides a visual and informative record of changes to the wound over time. Finally, wound measurement software provides all the features necessary to work with databases, such as entering new data, and modifying or viewing existing data.","PeriodicalId":346624,"journal":{"name":"The Second Asian and Pacific Rim Symposium on Biophotonics, 2004. APBP 2004.","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2004-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117094786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2004-12-15DOI: 10.1109/APBP.2004.1412363
C.Y. Han, Y. Chao, C.L. Lee
A photoelastic modulated ellipsometry combined with SPR technique is proposed. In addition to monitor the biological interactions in real time, we also installed a post flight analysis technique to analyze the same system synchronously.
{"title":"Ultrafast surface plasmon resonance biological detection by means of photoelastic modulated ellipsometry","authors":"C.Y. Han, Y. Chao, C.L. Lee","doi":"10.1109/APBP.2004.1412363","DOIUrl":"https://doi.org/10.1109/APBP.2004.1412363","url":null,"abstract":"A photoelastic modulated ellipsometry combined with SPR technique is proposed. In addition to monitor the biological interactions in real time, we also installed a post flight analysis technique to analyze the same system synchronously.","PeriodicalId":346624,"journal":{"name":"The Second Asian and Pacific Rim Symposium on Biophotonics, 2004. APBP 2004.","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2004-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115150079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2004-12-15DOI: 10.1109/APBP.2004.1412281
P. Lou
Studies in the past have confirmed the safety and efficacy of photodynamic therapy (PDT) in the treatment of various human cancers. Because of the concern for cosmetic and functional preservation, PDT seems to be particularly suited for head and neck tumor treatment. Results from previous studies are promising. Good cosmetic and functional outcomes can be achieved without compromising the patients' survival. PDT is a therapeutic option that may prove a useful addition to the armamentarium of the integrated head and neck oncology team.
{"title":"Photodynamic therapy for head and neck tumors","authors":"P. Lou","doi":"10.1109/APBP.2004.1412281","DOIUrl":"https://doi.org/10.1109/APBP.2004.1412281","url":null,"abstract":"Studies in the past have confirmed the safety and efficacy of photodynamic therapy (PDT) in the treatment of various human cancers. Because of the concern for cosmetic and functional preservation, PDT seems to be particularly suited for head and neck tumor treatment. Results from previous studies are promising. Good cosmetic and functional outcomes can be achieved without compromising the patients' survival. PDT is a therapeutic option that may prove a useful addition to the armamentarium of the integrated head and neck oncology team.","PeriodicalId":346624,"journal":{"name":"The Second Asian and Pacific Rim Symposium on Biophotonics, 2004. APBP 2004.","volume":"15 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2004-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125565755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2004-12-15DOI: 10.1109/APBP.2004.1412293
T. Peng, M. Jou
Images of mitochondria inside living cells with high spatial and temporal resolution could be visualized with specific functional fluorescent probes coupled with laser scanning confocal microscope. The interactions between mitochondria and other parts of cells remain nature and unaltered with this method. The effects of mitochondrial function changes on other parts of cells can best be investigated with this approach.
{"title":"Imaging mitochondria in living cells by confocal fluorescence microscopy","authors":"T. Peng, M. Jou","doi":"10.1109/APBP.2004.1412293","DOIUrl":"https://doi.org/10.1109/APBP.2004.1412293","url":null,"abstract":"Images of mitochondria inside living cells with high spatial and temporal resolution could be visualized with specific functional fluorescent probes coupled with laser scanning confocal microscope. The interactions between mitochondria and other parts of cells remain nature and unaltered with this method. The effects of mitochondrial function changes on other parts of cells can best be investigated with this approach.","PeriodicalId":346624,"journal":{"name":"The Second Asian and Pacific Rim Symposium on Biophotonics, 2004. APBP 2004.","volume":"14 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2004-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125581475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2004-12-15DOI: 10.1109/APBP.2004.1412332
Chih-Er Wu, S. Hua, S. Chu, Zhi-Yu Chen, Jiu-Chong Yuan, H. Chiang
We present autofluorescence modeling of different cervix tissue dysplasia for clinical diagnosis. We used double integrating sphere system and inverse adding-doubling (IAD) method to evaluate tissue optical parameters (absorption coefficient, /spl mu//sub a/, scattering coefficient, /spl mu//sub s/) of different cervix dysplasia. We used fluorescence Monte Carlo model included absorption coefficient and scattering coefficient from IAD and integrate sphere, to simulate autofluorescence spectra. The results show the increasing thickness of abnormal tissue on the cervical epithelium accompanied with the decreasing in autofluorescence intensity, in collagen peak (390 nm) and in NADH peak (460 nm), along with the lesion developments.
{"title":"Monte Carlo autofluorescence modeling on cervical dysplasia","authors":"Chih-Er Wu, S. Hua, S. Chu, Zhi-Yu Chen, Jiu-Chong Yuan, H. Chiang","doi":"10.1109/APBP.2004.1412332","DOIUrl":"https://doi.org/10.1109/APBP.2004.1412332","url":null,"abstract":"We present autofluorescence modeling of different cervix tissue dysplasia for clinical diagnosis. We used double integrating sphere system and inverse adding-doubling (IAD) method to evaluate tissue optical parameters (absorption coefficient, /spl mu//sub a/, scattering coefficient, /spl mu//sub s/) of different cervix dysplasia. We used fluorescence Monte Carlo model included absorption coefficient and scattering coefficient from IAD and integrate sphere, to simulate autofluorescence spectra. The results show the increasing thickness of abnormal tissue on the cervical epithelium accompanied with the decreasing in autofluorescence intensity, in collagen peak (390 nm) and in NADH peak (460 nm), along with the lesion developments.","PeriodicalId":346624,"journal":{"name":"The Second Asian and Pacific Rim Symposium on Biophotonics, 2004. APBP 2004.","volume":"6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2004-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133772413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：