Journal of Cleaner Production最新文献_第8页

Development of an innovative biocomposite using coconut fibres and bio-based binder for thermal and acoustic aplications in buildings

IF 9.7 1区环境科学与生态学 Q1 ENGINEERING, ENVIRONMENTAL

Journal of Cleaner Production

Pub Date : 2025-02-01 DOI: 10.1016/j.jclepro.2025.144834

António Figueiredo , German Vela , Guilherme Ascensão , Egon Vettorazzi , Romeu Vicente , Mónica Oliveira

The continuous demand for lightweight porous structures, with enhanced thermal and acoustic properties has been an important driver for the exploration of sustainable and eco-friendly materials for such applications. This research work presents the development of sustainable and improved thermal and acoustic biocomposite solution based on coconut fibres and sodium alginate designed for building applications. Different mix designs have been examined to investigate the effects of water and binder content on the mechanical performance of the samples. The biocomposite developed exhibited a maximum flexural stress and compressive stress at 10% deformation of 1 and 1.13 N mm⁻² respectively, revealing a superior performance when compared to conventional mineral wool solutions used in construction. Based on the mechanical performance, a biocomposite formulation was selected for thermal and acoustic characterization. Additionally, samples with different surface geometries have been produced for improved acoustic performance. Thermal characterization revealed a thermal conductivity value of 0.091 W m⁻¹ K⁻¹, while a sound absorption averages (SAA) ranging between 0.46 and 0.62 was obtained, making the developed material a well-absorptive solution suitable for reverberation and echo attenuation applications, whilst offering good thermal insulation properties. The development of this innovative biocomposite highlights that a dual purpose bio-based insulation solution can be effectively produced with coconut fibre and sodium alginate, with competitive thermal and acoustic insulation properties, constituting a promising and sustainable alternative to conventional insulation solutions used in construction.

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引用次数: 0

Does cross-policy mix work on digital innovation: Analyzing by the impact of open government data and low-carbon city pilot policies in China

IF 9.7 1区环境科学与生态学 Q1 ENGINEERING, ENVIRONMENTAL

Journal of Cleaner Production

Pub Date : 2025-02-01 DOI: 10.1016/j.jclepro.2025.144842

Lulu Wang , Yong Zhan

This paper examines the influence of policy combinations on digital innovation, drawing upon comprehensive panel data encompassing 284 Chinese cities. It focuses on China's open government data (OGD) and low-carbon city pilot (LCCP) policies to determine whether these policies, when combined, can elicit a synergistic effect that significantly facilitates digital innovation. Based on the “Technology-Organization-Environment” (TOE) framework, the empirical results indicate that the dual pilot policy combination substantially fosters digital innovation, with R&D diffusion, human capital diffusion, and public environmental concern serving as pivotal transmission mechanisms. The empirical evidence also demonstrates that the positive impact of the policy combinations of dual pilot cities on digital innovation varies depending on geographical location, urban administrative level, and city size. Moreover, building upon the new economic geography theory, the empirical results indicate that the spatial spillage of the dual pilot policy has a positive impact on digital innovation, with a spillover boundary of 250 km. These insights underscore the importance of strategic policy combinations and can guide governments in designing effective digital innovation incentive policies.

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引用次数: 0

Sustainable design of biomass valorization processes for the production of gamma valerolactone integrating wind energy and green hydrogen 整合风能和绿色氢的伽马戊内酯生产生物质增值过程的可持续设计

IF 9.7 1区环境科学与生态学 Q1 ENGINEERING, ENVIRONMENTAL

Journal of Cleaner Production

Pub Date : 2025-02-01 DOI: 10.1016/j.jclepro.2025.144829

Daniele Di Menno Di Bucchianico , Giordano Emrys Scarponi , Jean-Christophe Buvat , Sébastien Leveneur , Valeria Casson Moreno

The transition to net zero emissions in the chemical and process industries relies on the integrated use of renewable raw materials (e.g. biomass) and energy sources. The objective of this work is to propose a methodology aimed at the sustainable integration of renewables within the framework of green chemistry processes, where lignocellulosic biomass waste is valorized to produce biochemicals. The novelty is in the sustainability analysis extended to the supply systems, which allows for an optimized integration of multiple renewable energy sources to meet energy and raw materials demands. A sustainability comparison among different configurations for the process and its supply systems was made using a Multiple-Criteria Decision Analysis that allows for a ranking based on economic, environmental, and safety impacts. A case study was developed to describe the potentiality of the proposed methodology, based on a waste lignocellulosic biomass valorization process for the production of gamma valerolactone. Several configurations were developed and compared. The best Levelized Cost of Product (i.e. 2.08 €/kg_GVL) was obtained when lignocellulosic biomass was used to also cover the heat demand of the process, the production of electricity occurs on-site using wind turbines, and green hydrogen was produced and stored on-site to manage peak-shavings and valley fillings. From the environmental standpoint, maximum impacts (2 kg_CO2eq/kg_GVL) result when conventional sources of energy were considered, i.e. natural gas for thermal supply and grid electricity. Looking at the safety aspects, hydrogen production and storage sections account for approximately 10% of the overall hazard profile of the process, while the remaining contribution comes from the gamma valerolactone production section. The robustness of the results was demonstrated using uncertainty and sensitivity assessment on the input parameters, based on a Monte-Carlo approach.

化学和加工工业向净零排放的过渡依赖于可再生原材料（如生物质）和能源的综合利用。这项工作的目标是提出一种在绿色化学过程框架内可持续整合可再生能源的方法，在绿色化学过程中，木质纤维素生物质废物被转化为生产生化物质。新颖之处在于可持续性分析扩展到供应系统，它允许多种可再生能源的优化整合，以满足能源和原材料的需求。采用基于经济、环境和安全影响的多标准决策分析，对工艺及其供应系统的不同配置进行了可持续性比较。一个案例研究被开发来描述所提出的方法的潜力，基于生产伽马戊内酯的废弃木质纤维素生物质增值过程。开发并比较了几种配置。当使用木质纤维素生物质来满足该过程的热量需求，使用风力涡轮机现场发电，并在现场生产和储存绿色氢气以管理削峰和山谷填充时，获得了最佳的产品平均成本（即2.08欧元/kgGVL）。从环境的角度来看，最大的影响（2 kgCO2eq/kgGVL）是在考虑常规能源时产生的，即用于供热和电网供电的天然气。从安全方面来看，氢气生产和储存部分约占该工艺整体危害概况的10%，而剩余的贡献来自伽马戊内酯生产部分。基于蒙特卡罗方法，通过对输入参数的不确定性和敏感性评估来证明结果的鲁棒性。

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引用次数: 0

Pilot-scale recovery of iron from refractory specularite ore using hydrogen-based mineral phase transformation technology: Process optimization, mineral characterization, and green production 利用氢基矿物相变技术从难熔镜铁矿中回收铁的中试规模：工艺优化、矿物特征和绿色生产

IF 9.7 1区环境科学与生态学 Q1 ENGINEERING, ENVIRONMENTAL

Journal of Cleaner Production

Pub Date : 2025-02-01 DOI: 10.1016/j.jclepro.2025.144836

Pengcheng Tian , Peng Gao , Mingxing Wang , Yanjun Li , Yuexin Han

Specularite, as a subspecies of hematite, is a potentially important iron resource, primarily distributed in South America. However, it is difficult to process by traditional methods due to their special properties. In this work, a study on specularite was conducted using grinding-hydrogen-based mineral phase transformation-magnetic separation technologies. The results showed that the iron concentrate produced in pilot-scale experiments meets excellent specifications, with a grade greater than 67% and recovery over 97%. In addition, the system operated with good stability, and its pollutant discharge complied with national standards. Following the further optimization of the process, the optimization process Ⅱ, namely magnetic separation-tailings with HMPT-magnetic separation, has demonstrated significant economic advantages. This process yielded an iron concentrate with a grade of 68.22% and a recovery of 97.20%, resulting in a net profit of about $9.75/h. Vibrating sample magnetometer (VSM) and scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDS) analyses were used to characterized the material and its products. The results indicate that the magnetic properties of specularite have significantly improved, and the ore structure become looser and more holes, thereby enhancing the separation of iron minerals from gangue minerals. In summary, this research provided a reliable method for the efficient, green, and low-cost utilization of specularite on an industrial scale in the future.

镜铁矿作为赤铁矿的一个亚种，是一种潜在的重要铁资源，主要分布在南美洲。然而，由于其特殊的性质，用传统方法难以加工。采用磨矿—氢基矿物相变—磁选工艺对镜铁矿进行了研究。结果表明，中试生产的铁精矿品位大于67%，回收率大于97%，达到了较好的指标。系统运行稳定性好，污染物排放符合国家标准。经过进一步的工艺优化，磁选—尾矿hmpt—磁选优化工艺Ⅱ具有明显的经济优势。该工艺获得的铁精矿品位为68.22%，回收率为97.20%，净利润约为9.75美元/小时。采用振动样品磁强计（VSM）、扫描电镜（SEM）和能谱分析（EDS）对材料及其产物进行了表征。结果表明：镜铁矿的磁性能明显改善，矿石结构疏松，孔洞增多，有利于铁矿物与脉石物的分离；综上所述，本研究为未来在工业规模上高效、绿色、低成本地利用镜铁矿提供了可靠的方法。

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引用次数: 0

ALTERNATIVE ASSESSMENT OF INDUSTRIAL EFFLUENT COMPLIANCE: A CASE STUDY OF STAKEHOLDERS’ PERSPECTIVES IN MALAYSIA

IF 11.1 1区环境科学与生态学 Q1 ENGINEERING, ENVIRONMENTAL

Journal of Cleaner Production

Pub Date : 2025-02-01 DOI: 10.1016/j.jclepro.2025.144776

Zulaikha Mokhtar, Steven Kenway, Ilje Pikaar

Safe and healthy waterways are essential for environmental sustainability, making the assessment of industrial effluent discharge compliance critical. This study investigates stakeholder perspectives on alternative rating assessments for measuring industry discharge compliance, addressing the current fragmentation in stakeholder involvement in Malaysia. A survey was conducted with 519 representatives from government agencies, non-governmental organizations, industries, environmental groups, and policymakers, analyzing six compliance rating assessments: planning and administration in legal compliance, industry and community empowerment, compliance operation, system implementation, environmental risk and emergency plan, and environmental audits. The findings reveal that 78% of participants support implementing new assessments, with system implementation, legal compliance, and compliance operation being the most accepted. Financial and tax incentives were identified as highly effective in encouraging industry participation in these assessments. Challenges such as financial constraints, compliance issues, manpower shortages, and a lack of awareness were also noted. This study not only provides insights into stakeholder perspectives but also highlights a promising path for future compliance management in Malaysia and beyond.

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引用次数: 0

A method to create weighted-average life cycle impact assessment results for construction products, and enable filtering throughout the design process 一种为建筑产品创建加权平均生命周期影响评估结果，并在整个设计过程中进行过滤的方法

IF 9.7 1区环境科学与生态学 Q1 ENGINEERING, ENVIRONMENTAL

Journal of Cleaner Production

Pub Date : 2025-02-01 DOI: 10.1016/j.jclepro.2024.144467

Ellen Marsh , Laura Hattam , Stephen Allen

The built environment contributes around 37% of global energy and process-related greenhouse gas emissions and requires significant decarbonisation to reach global net-zero targets. This means that the existing markets for construction products need to change drastically. Life cycle assessment (LCA) is critical in calculating the environmental impacts of construction products, element designs (such as facades) and buildings. The initial stages of a project create the best opportunity to minimise the final design's environmental impact.

A wealth of environmental product declaration (EPD) data is now available. However, EPDs are often too specific for the early stages of projects where specifications are unknown. Building designers, therefore, need better representative data for early-stage LCA, where there is currently a lack of generic data for construction materials and newly emerging novel products.

Weighted averages from individual datasets weighted by market share (production volume), can help to bridge this data gap. Our new framework for creating weighted average datasets, incorporating uncertainty for an example material – steel. For the first time, these weighted averages consider uncertainty in both production volume (market) and LCI datasets. The ability to aggregate individual datasets into weighted averages could empower designers to make more confident early-stage decisions despite incomplete LCA data. And with the data categorisation and filtering criteria, the weighted values can be uniquely defined, with the option to increase the specificity of the average value as assessments become more certain.

Finally, if production volumes and uncertainty information were included in EPDs, the method given in this paper could unlock the potential in the rapidly growing library of construction material EPDs to support early-stage LCA.

建筑环境排放的温室气体约占全球能源和工艺相关排放总量的 37%，要实现全球净零排放目标，就必须大幅降低碳排放。这意味着现有的建筑产品市场需要发生巨大变化。生命周期评估（LCA）对于计算建筑产品、元素设计（如外墙）和建筑物对环境的影响至关重要。项目的初始阶段是将最终设计的环境影响降至最低的最佳时机。然而，对于规格未知的项目早期阶段来说，环境产品声明往往过于具体。因此，建筑设计师需要更具代表性的数据来进行早期生命周期分析，而目前缺乏建筑材料和新兴新产品的通用数据。我们为创建加权平均数据集制定了新的框架，并将不确定性纳入了一种示例材料--钢材。这些加权平均值首次同时考虑了产量（市场）和 LCI 数据集的不确定性。将单个数据集汇总为加权平均值的能力可使设计师在生命周期评估数据不完整的情况下，仍能做出更有把握的早期决策。最后，如果将产量和不确定性信息包含在 EPD 中，本文给出的方法可以释放快速增长的建筑材料 EPD 库中的潜力，为早期生命周期评估提供支持。

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引用次数: 0

Evaluation of resources, environment, and ecological carrying capacity from the perspective of “production-living-ecology” spaces: A case study of western Jilin Province, China “生产-生活-生态”空间视角下的资源环境生态承载力评价——以吉林省西部为例

IF 9.7 1区环境科学与生态学 Q1 ENGINEERING, ENVIRONMENTAL

Journal of Cleaner Production

Pub Date : 2025-02-01 DOI: 10.1016/j.jclepro.2025.144770

Yi Bole , Su Rina , Suri Guga , Mula Na , Shimeng Fan , Jiquan Zhang

China's rapid socioeconomic development and increasing consumption of resources has greatly intensified environmental pollution and ecological degradation across the country over recent decades. Regional differences and interactions among various elements enhance the complexity of addressing these issues, which not only makes resource and environmental management challenging, but also requires flexible and dynamic management strategies to cope with multilevel and multidimensional conflicts and uncertainties. Moreover, effective management of resources and environmental impacts requires the integration of numerous factors and adaptative strategies to respond to the continuously changing environmental conditions and evolving societal needs. Here, we introduce a conceptual framework and evaluation index system for resource and environmental carrying capacity (REECC) from “production-living-ecology” spaces (PLES) perspective. We used this framework to quantitatively evaluate REECC in the western Jilin Province and to conduct in-depth analyses of its internal drivers and climate impacts. The REECC and direction of movement of the center of gravity of different land-use types were also assessed. Spatial and temporal changes in REECC in this region over the period of 2005–2020 were particularly striking. In 2005, areas with an REECC between 0.4 and 0.5 accounted for 47.89% of total areas, but this fell to 37.16% in 2010 before leveling off at 41.76% in 2015–2020. Population density was the primary influencing factor, followed by EVI and PM2.5. Precipitation was significantly positively correlated with REECC, whereas temperature was significantly negatively correlated with REECC. The direction of the REECC trend was found to be consistent with that for grasslands, and the position of the center of gravity was shifted.

近几十年来，中国社会经济的快速发展和资源消耗的不断增加，大大加剧了全国的环境污染和生态退化。区域差异和各要素之间的相互作用增加了解决这些问题的复杂性，这不仅使资源和环境管理具有挑战性，而且需要灵活动态的管理策略来应对多层次、多维度的冲突和不确定性。此外，资源和环境影响的有效管理需要综合许多因素和适应性战略，以应对不断变化的环境条件和不断变化的社会需求。本文从“生产-生活-生态”空间的角度，提出了资源环境承载力的概念框架和评价指标体系。利用该框架对吉林西部地区REECC进行了定量评价，并对其内部驱动因素和气候影响进行了深入分析。并对不同土地利用类型的REECC和重心移动方向进行了评价。2005-2020年该区域REECC的时空变化尤为显著。2005年，REECC在0.4-0.5之间的地区占总面积的47.89%，但2010年下降到37.16%，2015-2020年稳定在41.76%。人口密度是主要影响因子，其次是EVI和PM2.5。降水量与REECC呈显著正相关，气温与REECC呈显著负相关。REECC趋势方向与草原一致，重心位置发生偏移。

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引用次数: 0

A modeling framework and benchmark for end-of-life automotive traction battery pack forecasting 汽车牵引电池组报废预测的建模框架和基准

IF 9.7 1区环境科学与生态学 Q1 ENGINEERING, ENVIRONMENTAL

Journal of Cleaner Production

Pub Date : 2025-02-01 DOI: 10.1016/j.jclepro.2025.144752

Max Rettenmeier , Dimitri Petrik , Mauritz Möller , Alexander Sauer

To meet climate targets, it is essential to establish a closed-loop system for the critical raw materials used in lithium-ion batteries, necessitating robust planning and forecasting methods for end-of-life battery volumes. Recycling end-of-life automotive batteries is crucial for establishing sustainable circulating flows of raw materials to reduce the use of virgin resources, particularly in the context of electromobility and energy storage. An accurate forecast of the availability of end-of-life automotive batteries for recycling is essential to determine the timing and the business potential of end-of-life battery treatment, driving the appropriate investments by policy makers and industry in battery recycling technologies and facilities. This paper presents a novel methodological framework incorporating multi-metric modeling with a distinct geographical focus and selected statistical approaches, collectively directed towards stakeholder-oriented end-of-life traction battery forecasts. We further benchmark the existing models based on the period under consideration, the geographical scope, the metrics employed, and the statistical techniques applied. This yields a novel recommendation framework for policy makers and industry to provide guidance on how to model end-of-life battery volumes. The recommendation framework links the required modeling approaches with relevant stakeholders, such as car repair shops, disassemblers, recyclers, logistics companies, and policy makers. The framework is validated through a case study for end-of-life automotive battery forecasting. The objective of this study is therefore not only to support the ramp-up of the battery recycling industry with a synthesized forecasting framework, but also to provide precise recommendations to the different industries. The findings of our study yield the core conclusion that global modelings with sophisticated statistical approaches, such as the Weibull approach, should be employed across a range of stakeholder-oriented metrics, including weight, capacity, and the number of end-of-life electric vehicle batteries.

为了实现气候目标，必须为锂离子电池中使用的关键原材料建立一个闭环系统，需要对报废电池数量进行强有力的规划和预测方法。回收报废汽车电池对于建立可持续的原材料循环流以减少原始资源的使用至关重要，特别是在电动汽车和能源储存的背景下。准确预测报废汽车电池的可回收性，对于确定报废电池处理的时机和商业潜力至关重要，从而推动政策制定者和行业对电池回收技术和设施的适当投资。本文提出了一种新的方法框架，将多度量模型与独特的地理焦点和选定的统计方法相结合，共同针对面向利益相关者的报废牵引电池预测。我们进一步根据所考虑的时期、地理范围、采用的指标和应用的统计技术对现有模型进行基准测试。这为政策制定者和行业提供了一个新的建议框架，为如何模拟报废电池的体积提供指导。建议框架将所需的建模方法与相关的利益相关者（如汽车修理店、拆解商、回收商、物流公司和政策制定者）联系起来。通过一个汽车电池报废预测的案例研究，验证了该框架的有效性。因此，本研究的目的不仅是通过综合预测框架支持电池回收行业的发展，而且还为不同的行业提供精确的建议。我们的研究结果得出了一个核心结论，即采用威布尔方法等复杂统计方法的全球模型，应该适用于一系列以利益相关者为导向的指标，包括重量、容量和报废电动汽车电池的数量。

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引用次数: 0

Future climate change will strengthen cotton production but have substantial environmental costs—A focus on Xinjiang by APSIM modelling 未来的气候变化将加强棉花生产，但也会带来巨大的环境成本——APSIM模型对新疆的关注

IF 9.7 1区环境科学与生态学 Q1 ENGINEERING, ENVIRONMENTAL

Journal of Cleaner Production

Pub Date : 2025-02-01 DOI: 10.1016/j.jclepro.2025.144803

Huiping Zhou , Jiabao Chen , Xiaowei Ding , Qihui Qin , Liebao Han

Assessing the potential impacts of climate change on cotton production, water consumption and carbon emissions is crucial for reducing water allocations and carbon emissions, especially in Xinjiang, a major cotton-producing region of China with an arid climate and water scarcity. In this study, we used the process-based crop model Agricultural Production Systems sIMulator (APSIM) to simulate the spatial and temporal variability of cotton yield, water consumption (ET), irrigation water requirement (IWR), irrigation water productivity (IWP), global warming potential (GWP), and greenhouse gas intensity (GHGI) in Xinjiang, and to estimate the total amount of cotton yield, water demand, and carbon emissions in North Xinjiang (NXJ) and South Xinjiang (SXJ). The results showed that the lint cotton yield in Xinjiang benefited from elevated temperature and CO₂ levels under SSP245 and peaked at 2334 kg/ha in the 2060s (14.1% higher than that in the historical period), while the positive effect of elevated CO₂ levels was eliminated by the rapid increase in temperature under SSP585 and peaked at 2219 kg/ha in the 2050s (11.6% higher than that in the historical period). Compared with their levels in 1981–2020, the IWR, GWP, and GHGI increased by 4.3–5.6%, 38.5–41.6%, and 25.1–25.8%, respectively, by the 2050s–2060s under SSP245 and SSP585, thus posing great challenges for water resource and carbon emission reductions in Xinjiang. Cotton production in SXJ would reach 2.87–3.24 million tons, dominating that in Xinjiang (55.1–56.2%) by the middle to end of this century but would cost 0.76–0.92 billion m³ more for irrigation and 0.28–0.59 million tons CO₂ eq more for GWP than in NXJ. These results suggest that future cotton production in Xinjiang will be limited by water and environmental pressures, especially after the 2060s, and that controlling carbon emissions in all industries and slowing the warming process will contribute to the sustainable development of cotton.

评估气候变化对棉花生产、用水量和碳排放的潜在影响对于减少水资源配置和碳排放至关重要，特别是在中国干旱缺水的棉花主产区新疆。本研究利用基于过程的作物模型农业生产系统模拟器（APSIM）对新疆棉花产量、耗水量（ET）、灌溉需水量（IWR）、灌溉水分生产力（IWP）、全球变暖潜势（GWP）和温室气体强度（GHGI）的时空变化进行了模拟，并估算了北疆（NXJ）和南疆（SXJ）棉花产量、需水量和碳排放总量。结果表明，SSP245处理下新疆皮棉产量受益于升温和CO2水平的提高，在20世纪60年代达到峰值2334 kg/ha（比历史时期高14.1%），而SSP585处理下CO2水平升高的积极影响被快速升温所抵消，在21世纪50年代达到峰值2219 kg/ha（比历史时期高11.6%）。与1981 ~ 2020年相比，到2050 ~ 2060年，SSP245和SSP585条件下的IWR、GWP和GHGI分别增长了4.3 ~ 5.6%、38.5 ~ 41.6%和25.1 ~ 25.8%，给新疆水资源和碳减排带来了巨大挑战。到本世纪中后期，新疆省棉花产量将达到287 - 324万吨，超过新疆（55.1-56.2%），但灌溉成本将比新疆省多0.76-0.92亿m³，GWP将比新疆省多0.28-0.59万吨CO2当量。这些结果表明，未来新疆棉花生产将受到水资源和环境压力的限制，特别是在20世纪60年代以后，控制所有行业的碳排放和减缓全球变暖进程将有助于棉花的可持续发展。

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引用次数: 0

Exploring the performance and mechanisms of Mychonastes rotundus in fixing CO2 based on multi-omics analysis 基于多组学分析探讨圆形真菌固定CO2的性能和机制

IF 9.7 1区环境科学与生态学 Q1 ENGINEERING, ENVIRONMENTAL

Journal of Cleaner Production

Pub Date : 2025-02-01 DOI: 10.1016/j.jclepro.2025.144820

Lanhe Zhang , Mingshuang Zhang , Jingbo Guo , Zihan Liu , Shuang Ji

Utilizing microalgae to immobilize carbon dioxide (CO₂) was regarded as one of the most environmentally friendly, safe and sustainable carbon capture, utilization and storage technologies. Mychonastes rotundus was a freshwater algae belonging to the Chlorophyceae. It had a wide range of habitats around the world, especially growing in streams and large stagnant water bodies. Mychonastes rotundus contained high levels of lipids and proteins, and could utilize various molecular forms of nutrients and light wavelengths for photosynthesis. However, the application of Mychonastes rotundus for carbon sequestration was rarely reported. In this study, CO₂ fixation efficiency and the physiological characteristics of Mychonastes rotundus at different CO₂ input concentrations (10%, 20% and 30%) were investigated, and its transcriptomics and metabolomics were reported for the first time. The results showed that Mychonastes rotundus achieved the highest and stablest carbon fixation efficiency and its cell hydrophobicity was the strongest at 20% CO₂. Compared with that without CO₂ input, Mychonastes rotundus upregulated 21444 genes and 188 metabolites, and genes encoding EC: 3.1.3.37 and EC: 1.2.1.13 in carbon fixation pathways upregulated. The most significant enrichment pathways were metabolic pathways and ABC transporters to resist cell damage caused by CO₂. However, the protein synthesis and material exchange between nucleus and cytoplasm were inhibited and ATPase activity decreased to 50% at 30% CO₂ concentration, leading to massive death of microalgae cells. This study provided a wide range of mechanistic insights into the CO₂ fixation process of Mychonastes rotundus at the genetic level.

利用微藻固定二氧化碳（CO2）被认为是最环保、安全、可持续的碳捕集、利用和封存技术之一。圆形密蚧是一种淡水藻，属于绿藻科。它在世界各地有广泛的栖息地，尤其是在溪流和大的停滞水体中生长。圆形真菌含有高水平的脂质和蛋白质，可以利用各种分子形式的营养物质和光波长进行光合作用。然而，圆形密霉在固碳方面的应用鲜有报道。本研究对不同CO2输入浓度（10%、20%和30%）下圆形米chonastes rotundus的CO2固定效率和生理特性进行了研究，并首次报道了其转录组学和代谢组学。结果表明，在CO2浓度为20%时，圆形真菌的固碳效率最高且稳定，其细胞疏水性最强。与未输入CO2相比，圆齿Mychonastes上调了21444个基因和188个代谢物，其中碳固定途径中编码EC: 3.1.3.37和EC: 1.2.1.13的基因上调。最重要的富集途径是代谢途径和ABC转运蛋白，以抵抗CO2引起的细胞损伤。然而，在30%的CO2浓度下，微藻细胞的蛋白质合成和细胞核与细胞质之间的物质交换受到抑制，atp酶活性下降至50%，导致微藻细胞大量死亡。本研究在遗传水平上对圆形米chonastes的CO2固定过程提供了广泛的机制见解。

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引用次数: 0