Journal of Functional Foods最新文献_第3页

Ellagic acid alleviates the progression of atherosclerosis in rats by inhibiting oxidative damage in endothelial cells 鞣花酸通过抑制内皮细胞氧化损伤减轻大鼠动脉粥样硬化的进展

IF 4 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Journal of Functional Foods

Pub Date : 2025-11-22 DOI: 10.1016/j.jff.2025.107105

Fang Shao , Shen Zeng , Zhiheng Wei , Elizabeth R. Gilbert , Jianfeng Yu , Lu Xu , Zhiliang Gu

The objective of this research was to examine the protective effects of ellagic acid (EA) against diabetes-induced atherosclerosis in rats and oxidative damage in vascular endothelial cells. In Type 2 diabetic rats induced by high-fat diet and streptozotocin (STZ), a four-week EA treatment noticeably reduced serum lipid deposition, improved oxidative stress indicators, alleviated aortic endothelial atherosclerosis, and restored the diversity and the composition of the gut microbiota. Correlation analysis demonstrated significant associations among oxidative damage markers and gut microbiota influenced by EA. RNA sequencing in EA-treated human umbilical vein endothelial cells (HUVECs) and network pharmacology identified NAD(P)H dehydrogenase quinone 1 (NQO1), heme oxygenase-1 (HO-1), and superoxide dismutase-2 (SOD2) as potential key antioxidant genes. Furthermore, experiments in a high-glucose or hydrogen peroxide (H₂O₂)-induced oxidative damage model in HUVECs confirmed that EA significantly enhanced cellular vitality, reduced cellular oxidative damage, and upregulated NQO1, HO-1, and SOD2 expression at both gene and protein levels. In summary, these findings demonstrate that EA mitigates diabetic atherosclerosis by enhancing endothelial antioxidant capacity and modulating gut microbiota.

本研究旨在探讨鞣花酸（EA）对糖尿病大鼠动脉粥样硬化和血管内皮细胞氧化损伤的保护作用。在高脂饮食和链脲佐菌素（STZ）诱导的2型糖尿病大鼠中，为期四周的EA治疗明显减少了血清脂质沉积，改善了氧化应激指标，缓解了主动脉内皮动脉粥样硬化，恢复了肠道微生物群的多样性和组成。EA对人脐静脉内皮细胞（HUVECs）的RNA测序和网络药理学研究发现，NAD(P)H脱氢酶醌1 （NQO1）、血红素氧化酶1 （HO-1）和超氧化物歧化酶2 （SOD2）是潜在的关键抗氧化基因。此外，在高糖或过氧化氢（H2O2）诱导的HUVECs氧化损伤模型中，实验证实，EA显著增强细胞活力，减少细胞氧化损伤，并在基因和蛋白水平上上调NQO1、HO-1和SOD2的表达。总之，这些研究结果表明，EA通过增强内皮抗氧化能力和调节肠道微生物群来减轻糖尿病动脉粥样硬化。

{"title":"Ellagic acid alleviates the progression of atherosclerosis in rats by inhibiting oxidative damage in endothelial cells","authors":"Fang Shao , Shen Zeng , Zhiheng Wei , Elizabeth R. Gilbert , Jianfeng Yu , Lu Xu , Zhiliang Gu","doi":"10.1016/j.jff.2025.107105","DOIUrl":"10.1016/j.jff.2025.107105","url":null,"abstract":"<div><div>The objective of this research was to examine the protective effects of ellagic acid (EA) against diabetes-induced atherosclerosis in rats and oxidative damage in vascular endothelial cells. In Type 2 diabetic rats induced by high-fat diet and streptozotocin (STZ), a four-week EA treatment noticeably reduced serum lipid deposition, improved oxidative stress indicators, alleviated aortic endothelial atherosclerosis, and restored the diversity and the composition of the gut microbiota. Correlation analysis demonstrated significant associations among oxidative damage markers and gut microbiota influenced by EA. RNA sequencing in EA-treated human umbilical vein endothelial cells (HUVECs) and network pharmacology identified NAD(<em>P</em>)H dehydrogenase quinone 1 (NQO1), heme oxygenase-1 (HO-1<em>)</em>, and superoxide dismutase-2 (SOD2) as potential key antioxidant genes. Furthermore, experiments in a high-glucose or hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>)-induced oxidative damage model in HUVECs confirmed that EA significantly enhanced cellular vitality, reduced cellular oxidative damage, and upregulated NQO1, HO-1, and SOD2 expression at both gene and protein levels. In summary, these findings demonstrate that EA mitigates diabetic atherosclerosis by enhancing endothelial antioxidant capacity and modulating gut microbiota.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"135 ","pages":"Article 107105"},"PeriodicalIF":4.0,"publicationDate":"2025-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145569119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification and molecular mechanisms of novel wound healing potential peptides from eggshell membrane hydrolysates assisted by an integrated in silico and in vitro approach 从蛋壳膜水解物中鉴定新型创面愈合潜力肽及其分子机制——基于硅和体外集成方法的研究

IF 4 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Journal of Functional Foods

Pub Date : 2025-11-21 DOI: 10.1016/j.jff.2025.107099

Yanan Gao , Na Zhang , Yi Li , Rong Zhang , Min Gao , Ya Zhang , Dan Jiang , Shuyan Liu

The aim of this study was to demonstrate the wound-healing activity of novel peptides derived from the eggshell membranes (ESM) of fresh chicken eggs and to explore the underlying mechanisms using integrated in silico and in vitro approaches. The ESM hydrolysates prepared by papain (PapH) exhibited the highest antioxidant and wound-healing activity. Three novel peptides, KDFPCPM (P1), DKDGPFRLC (P2), and LVEKGDVAF (P3), were identified from PapH via peptidomics and subsequently synthesized. These synthetic peptides also displayed excellent antioxidant and wounding-healing effects, with P1 surpassed the other peptides. Molecular docking results indicated that P1 could form stable bonds with Keap1, a key antioxidant target identified through proteomics. Furthermore, in vitro experiments utilizing the Nrf2 inhibitor ML385 revealed that P1 from ESM promotes cell migration by activating the Keap1-mediated Nrf2/HO-1 signaling pathway, thereby enhancing the cellular antioxidant defense system. Collectively, these findings provided new insights for the high-value utilization of discarded ESM.

本研究的目的是证明从新鲜鸡蛋的蛋壳膜（ESM）中提取的新型肽的伤口愈合活性，并利用集成的硅和体外方法探索其潜在机制。木瓜蛋白酶（PapH）制备的ESM水解物具有最高的抗氧化和创面愈合活性。通过肽组学方法从PapH中鉴定出KDFPCPM （P1）、DKDGPFRLC （P2）和LVEKGDVAF （P3）三个新肽，并随后合成。这些合成肽也表现出良好的抗氧化和伤口愈合作用，其中P1优于其他肽。分子对接结果表明P1可以与Keap1形成稳定的键，Keap1是通过蛋白质组学鉴定的一个关键抗氧化靶点。此外，利用Nrf2抑制剂ML385进行的体外实验表明，ESM中的P1通过激活keap1介导的Nrf2/HO-1信号通路来促进细胞迁移，从而增强细胞的抗氧化防御系统。总的来说，这些发现为废弃ESM的高价值利用提供了新的见解。

{"title":"Identification and molecular mechanisms of novel wound healing potential peptides from eggshell membrane hydrolysates assisted by an integrated in silico and in vitro approach","authors":"Yanan Gao , Na Zhang , Yi Li , Rong Zhang , Min Gao , Ya Zhang , Dan Jiang , Shuyan Liu","doi":"10.1016/j.jff.2025.107099","DOIUrl":"10.1016/j.jff.2025.107099","url":null,"abstract":"<div><div>The aim of this study was to demonstrate the wound-healing activity of novel peptides derived from the eggshell membranes (ESM) of fresh chicken eggs and to explore the underlying mechanisms using integrated <em>in silico</em> and <em>in vitro</em> approaches. The ESM hydrolysates prepared by papain (PapH) exhibited the highest antioxidant and wound-healing activity. Three novel peptides, KDFPCPM (P1), DKDGPFRLC (P2), and LVEKGDVAF (P3), were identified from PapH <em>via</em> peptidomics and subsequently synthesized. These synthetic peptides also displayed excellent antioxidant and wounding-healing effects, with P1 surpassed the other peptides. Molecular docking results indicated that P1 could form stable bonds with Keap1, a key antioxidant target identified through proteomics. Furthermore, <em>in vitro</em> experiments utilizing the Nrf2 inhibitor ML385 revealed that P1 from ESM promotes cell migration by activating the Keap1-mediated Nrf2/HO-1 signaling pathway, thereby enhancing the cellular antioxidant defense system. Collectively, these findings provided new insights for the high-value utilization of discarded ESM.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"135 ","pages":"Article 107099"},"PeriodicalIF":4.0,"publicationDate":"2025-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145569121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sika deer bone peptide attenuates ovariectomy-induced osteoporosis in mice via PPAR-γ/Axin2/Wnt signaling pathway 梅花鹿骨肽通过PPAR-γ/Axin2/Wnt信号通路减轻小鼠卵巢切除所致骨质疏松

IF 4 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Journal of Functional Foods

Pub Date : 2025-11-21 DOI: 10.1016/j.jff.2025.107108

Guangchen Liu , Yue Ma , Guangxin Yuan , Hang Zhao , Ruofeng Yin

Sika deer bone peptides (DBP-1-3) were extracted and purified from fresh deer bones. In ovariectomized (OVX) osteoporotic mice, DBP-1-3 increased serum calcitonin (CT), estradiol (E2), and 1,25(OH)₂D₃ while reducing Ca²⁺ and bone gla protein (BGP). Micro-CT analysis revealed improved trabecular bone structure, with increased bone volume/total volume (BV/TV), trabecular number (Tb.N), and thickness (Tb.Th), and decreased trabecular separation (Tb.Sp). Mechanistically, DBP-1-3 downregulated PPAR-γ and Axin2 while upregulating β-catenin, suggesting inhibition of the PPAR-γ/Axin2 pathway and activation of Wnt signaling. These findings indicate that DBP-1-3 alleviates osteoporosis by modulating bone metabolism and enhancing bone microstructure, potentially via PPAR-γ/Axin2/Wnt regulation.

从新鲜梅花鹿骨中提取纯化梅花鹿骨肽（DBP-1-3）。在卵巢切除（OVX）骨质疏松小鼠中，DBP-1-3增加血清降钙素（CT）、雌二醇（E2）和1,25(OH)2D3，同时降低Ca2+和骨玻璃蛋白（BGP）。显微ct分析显示骨小梁结构改善，骨体积/总体积（BV/TV）增加，骨小梁数（Tb）增加。N)，厚度(Tb。Th)，小梁分离减少（Tb.Sp）。在机制上，DBP-1-3下调PPAR-γ和Axin2，上调β-catenin，提示抑制PPAR-γ/Axin2通路和激活Wnt信号。这些发现表明，DBP-1-3可能通过调控PPAR-γ/Axin2/Wnt调控骨代谢和增强骨微观结构来缓解骨质疏松症。

引用次数: 0

Effects of alkaline extraction and ultrasonication on digestibility, nutritional quality, and bioactive peptide release of duckweed (Wolffia arrhiza) protein extracts 碱性提取和超声处理对浮萍蛋白提取物消化率、营养品质及活性肽释放的影响

IF 4 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Journal of Functional Foods

Pub Date : 2025-11-21 DOI: 10.1016/j.jff.2025.107110

Saowakon Wongsasulak , Chotika Nitiwuttithorn , Punchira Vongsawasdi , Apiradee Hongsthong , Jirawat Yongsawatdigul

Duckweed is a promising sustainable protein source; however, its dense structure and anti-nutritional factors (e.g., saponins and tannins) may limit protein digestibility, nutritional, and biofunctional qualities. This study compared duckweed protein extracts (DPE) produced by alkaline extraction (AE), ultrasound-assisted alkaline extraction (UAAE), and ultrasound-assisted water extraction (UAWE) to native duckweed powder (DPw) regarding protein digestibility, antioxidant capacity, and bioactive peptide (BAP) release during in vitro gastrointestinal digestion. DPw exhibited the lowest digestibility, whereas DPE_UAE showed the highest, with UAWE achieving a DIAAS of 0.75, meeting FAO criteria for good protein quality. Enhanced digestibility strongly correlated with increased BAP release; DPE_UAE boosted long-chain BAPs by 1.4-fold and short-chain BAPs by 1.8-fold versus DPw, without altering bioactivity distribution. Furthermore, DPE_UAE demonstrated superior antioxidant capacity, with ACE and DPP-4 inhibitory activities as the top-two predominant bioactivities. UAE effectively produces DPE, demonstrating potential as a functional protein ingredient for food and dietary supplement applications.

浮萍是一种很有前途的可持续蛋白质来源；然而，其致密的结构和抗营养因子（如皂苷和单宁）可能会限制蛋白质的消化率、营养和生物功能品质。本研究比较了碱提法（AE）、超声辅助碱提法（UAAE）和超声辅助水提法（UAWE）制备的浮萍蛋白提取物（DPE）与天然浮萍粉（DPw）在体外胃肠道消化过程中蛋白质消化率、抗氧化能力和生物活性肽（BAP）释放方面的差异。DPw的消化率最低，而DPEUAE的消化率最高，UAWE的DIAAS为0.75，符合粮农组织的优质蛋白质标准。消化率提高与BAP释放增加密切相关；与DPw相比，DPEUAE使长链BAPs提高了1.4倍，短链BAPs提高了1.8倍，而生物活性分布没有改变。此外，DPEUAE还表现出较强的抗氧化能力，其中ACE和DPP-4抑制活性居前两位。阿联酋有效地生产DPE，展示了作为食品和膳食补充剂应用的功能性蛋白质成分的潜力。

{"title":"Effects of alkaline extraction and ultrasonication on digestibility, nutritional quality, and bioactive peptide release of duckweed (Wolffia arrhiza) protein extracts","authors":"Saowakon Wongsasulak , Chotika Nitiwuttithorn , Punchira Vongsawasdi , Apiradee Hongsthong , Jirawat Yongsawatdigul","doi":"10.1016/j.jff.2025.107110","DOIUrl":"10.1016/j.jff.2025.107110","url":null,"abstract":"<div><div>Duckweed is a promising sustainable protein source; however, its dense structure and anti-nutritional factors (e.g., saponins and tannins) may limit protein digestibility, nutritional, and biofunctional qualities. This study compared duckweed protein extracts (DPE) produced by alkaline extraction (AE), ultrasound-assisted alkaline extraction (UAAE), and ultrasound-assisted water extraction (UAWE) to native duckweed powder (DPw) regarding protein digestibility, antioxidant capacity, and bioactive peptide (BAP) release during in vitro gastrointestinal digestion. DPw exhibited the lowest digestibility, whereas DPE<sub>UAE</sub> showed the highest, with UAWE achieving a DIAAS of 0.75, meeting FAO criteria for good protein quality. Enhanced digestibility strongly correlated with increased BAP release; DPE<sub>UAE</sub> boosted long-chain BAPs by 1.4-fold and short-chain BAPs by 1.8-fold versus DPw, without altering bioactivity distribution. Furthermore, DPE<sub>UAE</sub> demonstrated superior antioxidant capacity, with ACE and DPP-4 inhibitory activities as the top-two predominant bioactivities. UAE effectively produces DPE, demonstrating potential as a functional protein ingredient for food and dietary supplement applications.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"135 ","pages":"Article 107110"},"PeriodicalIF":4.0,"publicationDate":"2025-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145569122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Digestion characteristics in vitro of polysaccharide from Pleurotus placentodes and its positive effects on microbiota 胎盘侧耳多糖体外消化特性及其对微生物群的积极影响

IF 4 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Journal of Functional Foods

Pub Date : 2025-11-20 DOI: 10.1016/j.jff.2025.107106

Zhenhua Yin , Hao Zhang , Juanjuan Zhang , Guangyu Hong , Xia Zhang , Qingfeng Guo , Wei Zhang , Li Wang , Lin Chen

Pleurotus placentodes, a nutritionally rich mushroom species within the Pleurotus spp. genus, contains rich polysaccharides. As its primary bioactive component, it has been rarely reported. This study provided the first report on in vitro digestion and fermentation properties of polysaccharide PPp-W through simulated digestion and anaerobic fermentation. The results showed that during simulated digestion, PPp-W resisted enzymatic degradation, as shown by its stable molecular weight and absence of free monosaccharide release. However, PPp-W was degraded and utilized by intestinal microorganisms, leading to the production of short-chain fatty acids (SCFAs), particularly acetic acid (increased 2.61 times) and propionic acid (increased 19.79 times), accompanied by a marked pH reduction in fecal culture medium. PPp-W administration significantly regulated the composition of intestinal flora by enhancing the relative abundance of beneficial bacteria (Bacteroides, Parabacteroides, and Lactobacillus) while suppressing harmful bacteria (Escherichia-Shigella and Enterococcus). These findings indicated that PPp-W exhibited significant prebiotic potential and represented a promising candidate for functional food applications targeting gut health modulation.

平菇是平菇属的一种营养丰富的蘑菇，含有丰富的多糖。作为其主要的生物活性成分，文献报道甚少。本研究首次通过模拟消化和厌氧发酵对多糖PPp-W的体外消化和发酵特性进行了研究。结果表明，在模拟消化过程中，PPp-W具有稳定的分子量和不释放游离单糖的特性，能够抵抗酶的降解。然而，PPp-W被肠道微生物降解利用，产生短链脂肪酸（SCFAs），特别是乙酸（增加2.61倍）和丙酸（增加19.79倍），粪便培养基pH值明显降低。PPp-W通过增加有益菌（拟杆菌、拟副杆菌和乳杆菌）的相对丰度，同时抑制有害菌（埃希氏志贺氏菌和肠球菌），显著调节肠道菌群的组成。这些发现表明，PPp-W具有显著的益生元潜力，并代表了一种有希望的功能性食品应用，以调节肠道健康。

{"title":"Digestion characteristics in vitro of polysaccharide from Pleurotus placentodes and its positive effects on microbiota","authors":"Zhenhua Yin , Hao Zhang , Juanjuan Zhang , Guangyu Hong , Xia Zhang , Qingfeng Guo , Wei Zhang , Li Wang , Lin Chen","doi":"10.1016/j.jff.2025.107106","DOIUrl":"10.1016/j.jff.2025.107106","url":null,"abstract":"<div><div><em>Pleurotus placentodes</em>, a nutritionally rich mushroom species within the <em>Pleurotus spp.</em> genus, contains rich polysaccharides. As its primary bioactive component, it has been rarely reported. This study provided the first report on <em>in vitro</em> digestion and fermentation properties of polysaccharide PPp-W through simulated digestion and anaerobic fermentation. The results showed that during simulated digestion, PPp-W resisted enzymatic degradation, as shown by its stable molecular weight and absence of free monosaccharide release. However, PPp-W was degraded and utilized by intestinal microorganisms, leading to the production of short-chain fatty acids (SCFAs), particularly acetic acid (increased 2.61 times) and propionic acid (increased 19.79 times), accompanied by a marked pH reduction in fecal culture medium. PPp-W administration significantly regulated the composition of intestinal flora by enhancing the relative abundance of beneficial bacteria (<em>Bacteroides</em>, <em>Parabacteroides</em>, and <em>Lactobacillus</em>) while suppressing harmful bacteria (<em>Escherichia</em>-<em>Shigella</em> and <em>Enterococcus</em>). These findings indicated that PPp-W exhibited significant prebiotic potential and represented a promising candidate for functional food applications targeting gut health modulation.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"135 ","pages":"Article 107106"},"PeriodicalIF":4.0,"publicationDate":"2025-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145569125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pharmacokinetics of liposomal multinutrients versus non-liposomal comparators in a randomized crossover trial 一项随机交叉试验中多营养素脂质体与非脂质体比较物的药代动力学

IF 4 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Journal of Functional Foods

Pub Date : 2025-11-19 DOI: 10.1016/j.jff.2025.107103

Young Min Woo , Keun Woo Lee , Andre Kim

Liposomal delivery systems are utilized to enhance the bioavailability of nutrients. In this randomized, double-blind, two-period crossover clinical trial, healthy Korean adults (n = 20) were enrolled to assess the absorption of liposomal multinutrients compared to composition-matched non-liposomal comparators. Two distinct liposomal multinutrient formulations (LMN-1 and LMN-2) were compared with two corresponding non-liposomal multinutrient products (NLMN-1 and NLMN-2). Pharmacokinetic (PK) parameters—including C_max, T_max, and incremental area under the curve (iAUC)—were analyzed for vitamin B3, vitamin C, zinc, and iron. Vitamin C and zinc concentrations were significantly higher from 1 to 8 h and 0–6 h, respectively, while iron concentrations were elevated at most time points for the liposomal group. No clinically significant adverse events were observed during the study period. These findings indicate that liposomal multinutrients improve nutrient absorption and demonstrate good tolerability in humans. The results support the application of liposomal formulations in functional foods as a strategy to enhance nutrient delivery.

脂质体输送系统被用来提高营养物质的生物利用度。在这项随机、双盲、两期交叉临床试验中，健康的韩国成年人（n = 20）被招募，以评估脂质体多营养素的吸收与成分匹配的非脂质体比较物的吸收。将两种不同的脂质体多营养素制剂（LMN-1和LMN-2）与两种相应的非脂质体多营养素制剂（NLMN-1和NLMN-2）进行了比较。分析了维生素B3、维生素C、锌和铁的药代动力学（PK）参数，包括C_max、T_max和增量曲线下面积（iAUC）。维生素C和锌浓度分别在1 ~ 8 h和0 ~ 6 h显著升高，铁浓度在大多数时间点均升高。在研究期间未观察到有临床意义的不良事件。这些发现表明，脂质体多营养素改善营养吸收，并表现出良好的耐受性。结果支持在功能性食品中应用脂质体制剂作为一种增强营养输送的策略。

{"title":"Pharmacokinetics of liposomal multinutrients versus non-liposomal comparators in a randomized crossover trial","authors":"Young Min Woo , Keun Woo Lee , Andre Kim","doi":"10.1016/j.jff.2025.107103","DOIUrl":"10.1016/j.jff.2025.107103","url":null,"abstract":"<div><div>Liposomal delivery systems are utilized to enhance the bioavailability of nutrients. In this randomized, double-blind, two-period crossover clinical trial, healthy Korean adults (<em>n</em> = 20) were enrolled to assess the absorption of liposomal multinutrients compared to composition-matched non-liposomal comparators. Two distinct liposomal multinutrient formulations (LMN-1 and LMN-2) were compared with two corresponding non-liposomal multinutrient products (NLMN-1 and NLMN-2). Pharmacokinetic (PK) parameters—including C_max, T_max, and incremental area under the curve (iAUC)—were analyzed for vitamin B3, vitamin C, zinc, and iron. Vitamin C and zinc concentrations were significantly higher from 1 to 8 h and 0–6 h, respectively, while iron concentrations were elevated at most time points for the liposomal group. No clinically significant adverse events were observed during the study period. These findings indicate that liposomal multinutrients improve nutrient absorption and demonstrate good tolerability in humans. The results support the application of liposomal formulations in functional foods as a strategy to enhance nutrient delivery.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"135 ","pages":"Article 107103"},"PeriodicalIF":4.0,"publicationDate":"2025-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145569123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rhubarb polysaccharides alleviate hypertriglyceridemia-induced acute pancreatitis and regulate gut microbiota-mediated tryptophan metabolism 大黄多糖减轻高甘油三酯血症诱导的急性胰腺炎和调节肠道微生物介导的色氨酸代谢

IF 4 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Journal of Functional Foods

Pub Date : 2025-11-19 DOI: 10.1016/j.jff.2025.107104

Ya Chen , Wen-Wen Zhang , Yun-Yun Mao , Gang-Ao Hu , Xin-Xin Chen , Yi-Lin Zhou , Xiao-Long Chen , Hong Wang , Bin Wei , Jian-Feng Tu

Rheum tanguticum plays a key role in treating acute pancreatitis, though its bioactive components and mechanisms remain unclear. This study investigates the structural characteristics and therapeutic effects of rhubarb polysaccharides (RP) in a mouse model of hypertriglyceridemia-induced acute pancreatitis (HTGAP). The isolated polysaccharide, (26 kDa, primarily glucose and arabinose) significantly reduced serum amylase and lipase levels, improved pancreatic and intestinal histopathology and upregulated tight junction proteins Zonula Occludens-1 and Occludin. High throughput 16S rRNA sequencing demonstrated that RP supplementation modulated gut microbiota by enriching beneficial genera (e.g., Lactobacillus and Akkermansia) while reducing pathogenic genera (Lachnoclostridium, Desulfovibrio). Untargeted metabolomics revealed alterations in microbial metabolites, particularly in the tryptophan metabolism. Integrated analysis revealed significant microbiota-metabolite correlations. These findings suggest that RP improve HTGAP by restoring intestinal barrier integrity and regulating gut microbiota-mediated tryptophan metabolism, highlighting its potential as a therapeutic agent.

大黄在治疗急性胰腺炎中起关键作用，但其生物活性成分和机制尚不清楚。本研究探讨了大黄多糖（RP）的结构特征及其对小鼠高甘油三酯血症诱导的急性胰腺炎（HTGAP）模型的治疗作用。分离的多糖（26 kDa，主要是葡萄糖和阿拉伯糖）显著降低血清淀粉酶和脂肪酶水平，改善胰腺和肠道组织病理学，上调紧密连接蛋白Zonula occluden -1和Occludin。高通量16S rRNA测序表明，RP补充通过丰富有益菌（如乳酸杆菌和Akkermansia）而减少致病菌（Lachnoclostridium, Desulfovibrio）来调节肠道微生物群。非靶向代谢组学揭示了微生物代谢物的变化，特别是色氨酸代谢。综合分析显示了显著的微生物群-代谢物相关性。这些研究结果表明，RP通过恢复肠道屏障完整性和调节肠道微生物介导的色氨酸代谢来改善HTGAP，突出了其作为治疗药物的潜力。

{"title":"Rhubarb polysaccharides alleviate hypertriglyceridemia-induced acute pancreatitis and regulate gut microbiota-mediated tryptophan metabolism","authors":"Ya Chen , Wen-Wen Zhang , Yun-Yun Mao , Gang-Ao Hu , Xin-Xin Chen , Yi-Lin Zhou , Xiao-Long Chen , Hong Wang , Bin Wei , Jian-Feng Tu","doi":"10.1016/j.jff.2025.107104","DOIUrl":"10.1016/j.jff.2025.107104","url":null,"abstract":"<div><div><em>Rheum tanguticum</em> plays a key role in treating acute pancreatitis, though its bioactive components and mechanisms remain unclear. This study investigates the structural characteristics and therapeutic effects of rhubarb polysaccharides (RP) in a mouse model of hypertriglyceridemia-induced acute pancreatitis (HTGAP). The isolated polysaccharide, (26 kDa, primarily glucose and arabinose) significantly reduced serum amylase and lipase levels, improved pancreatic and intestinal histopathology and upregulated tight junction proteins Zonula Occludens-1 and Occludin. High throughput 16S rRNA sequencing demonstrated that RP supplementation modulated gut microbiota by enriching beneficial genera (e.g., <em>Lactobacillus</em> and <em>Akkermansia</em>) while reducing pathogenic genera (<em>Lachnoclostridium</em>, <em>Desulfovibrio</em>). Untargeted metabolomics revealed alterations in microbial metabolites, particularly in the tryptophan metabolism. Integrated analysis revealed significant microbiota-metabolite correlations. These findings suggest that RP improve HTGAP by restoring intestinal barrier integrity and regulating gut microbiota-mediated tryptophan metabolism, highlighting its potential as a therapeutic agent.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"135 ","pages":"Article 107104"},"PeriodicalIF":4.0,"publicationDate":"2025-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145569124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oat and lentil-based muffins as a functional food: Enhanced glycaemia control, anti-inflammatory effects, and intestinal epithelium integrity 燕麦和扁豆为基础的松饼作为一种功能性食品：增强血糖控制，抗炎作用，和肠上皮完整性

IF 4 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Journal of Functional Foods

Pub Date : 2025-11-17 DOI: 10.1016/j.jff.2025.107101

Rafaela Geraldo , Manuela Machado , Sara Silva , Simão Pinho , Alexandra Gouveia , Delminda Neves , Elisabete Pinto , Manuela Pintado , Marta W. Vasconcelos

The increased interest in functional foods has driven research into health-promoting properties. This study evaluated muffins made with 100 % oatmeal (OM) or a 50:50 lentil/oatmeal flour mix (LM) using the INFOGEST in vitro model. Analysis included starch composition, glycaemic response, cytotoxicity, and immunomodulatory effects in Caco-2 cells under basal and stimulated conditions. Digestion revealed limited hydrolysis in oral/gastric phases, with increase in intestinal phase. OM exhibited 8.9 % higher starch and greater hydrolysis. In contrast, LM presented 9.3 % lower estimated glycaemic index. Both muffins were non-toxic. Under stimulation, LM showed stronger anti-inflammatory effects, decreasing interleukin-6/8 mRNA and cytokine levels, while upregulating Transforming Growth Factor-β under basal conditions. LM enhanced barrier integrity, reduced Acetyl-Coenzyme A acetyltransferase 2, and upregulated Peroxisome proliferator-activated receptor-γ and Sirtuin 1, indicating improved metabolic regulation. These findings highlight lentil/oatmeal muffins as functional options that may support glycaemic control and intestinal health, aiding strategies against diabetes and cardiovascular disease.

人们对功能性食品兴趣的增加推动了对健康促进特性的研究。本研究使用INFOGEST体外模型评估了100%燕麦（OM）或50:50扁豆/燕麦粉混合物（LM）制成的松饼。分析包括淀粉组成、血糖反应、细胞毒性和Caco-2细胞在基础和刺激条件下的免疫调节作用。消化显示在口服/胃期水解有限，肠期水解增加。OM淀粉含量高8.9%，水解程度高。相比之下，LM的血糖指数降低了9.3%。两种松饼都是无毒的。在刺激条件下，LM表现出较强的抗炎作用，降低白细胞介素6/8 mRNA和细胞因子水平，上调基础条件下的转化生长因子-β水平。LM增强屏障完整性，降低乙酰辅酶A乙酰转移酶2，上调过氧化物酶体增殖物激活受体-γ和Sirtuin 1，表明代谢调节得到改善。这些发现强调了扁豆/燕麦松饼作为一种功能性选择，可能有助于血糖控制和肠道健康，有助于对抗糖尿病和心血管疾病。

{"title":"Oat and lentil-based muffins as a functional food: Enhanced glycaemia control, anti-inflammatory effects, and intestinal epithelium integrity","authors":"Rafaela Geraldo , Manuela Machado , Sara Silva , Simão Pinho , Alexandra Gouveia , Delminda Neves , Elisabete Pinto , Manuela Pintado , Marta W. Vasconcelos","doi":"10.1016/j.jff.2025.107101","DOIUrl":"10.1016/j.jff.2025.107101","url":null,"abstract":"<div><div>The increased interest in functional foods has driven research into health-promoting properties. This study evaluated muffins made with 100 % oatmeal (OM) or a 50:50 lentil/oatmeal flour mix (LM) using the INFOGEST <em>in vitro</em> model. Analysis included starch composition, glycaemic response, cytotoxicity, and immunomodulatory effects in Caco-2 cells under basal and stimulated conditions. Digestion revealed limited hydrolysis in oral/gastric phases, with increase in intestinal phase. OM exhibited 8.9 % higher starch and greater hydrolysis. In contrast, LM presented 9.3 % lower estimated glycaemic index. Both muffins were non-toxic. Under stimulation, LM showed stronger anti-inflammatory effects, decreasing interleukin-6/8 mRNA and cytokine levels, while upregulating <em>Transforming Growth Factor-β</em> under basal conditions. LM enhanced barrier integrity, reduced <em>Acetyl-Coenzyme A acetyltransferase 2</em>, and upregulated <em>Peroxisome proliferator-activated receptor-γ</em> and <em>Sirtuin 1</em>, indicating improved metabolic regulation. These findings highlight lentil/oatmeal muffins as functional options that may support glycaemic control and intestinal health, aiding strategies against diabetes and cardiovascular disease.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"135 ","pages":"Article 107101"},"PeriodicalIF":4.0,"publicationDate":"2025-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145569127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exocarpium Citri Grandis alleviates postmenopausal obesity in ovariectomized mice through gut microbiota remodeling and metabolic reprogramming 大香橼通过肠道菌群重塑和代谢重编程减轻卵巢切除小鼠绝经后肥胖

IF 4 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Journal of Functional Foods

Pub Date : 2025-11-17 DOI: 10.1016/j.jff.2025.107102

Tingting Chen , Xiaoqin Wu , Like Xu , Jiaxiong Zhao , Weiru Liu , Jiangtao Zeng , Zhuohui Luo , Jiawen Huang

Exocarpium Citri Grandis (ECG, Huajuhong), the epicarp of Citrus grandis ‘Tomentosa’, traditionally used to resolve phlegm, is also applied against phlegm-dampness-type obesity. Further evidence is needed to clarify its anti-obesity effects and role in gut microbiota modulation. In this study, ECG significantly reduced body weight, serum TG, TC, and LDL-c, while HDL-c increased (p < 0.05). Metabolic cage, qPCR, and Western blot analyses of ingWAT, pgWAT, and iBAT showed increased energy expenditure, upregulated thermogenic genes (e.g., Ucp1, Atp2a2), downregulated lipid synthesis genes (e.g., Acc, Fas), and enhanced lipolytic and mitochondrial gene expression (e.g., Atgl, Mgll).It also showed strong anti-inflammatory effects by reducing TNF-α, IL-6, and IL-1β, increasing mucus layer thickness, and upregulating intestinal barrier genes (Zo-1, Occludin).16S rDNA sequencing revealed increased β-diversity, SCFA-producing bacteria, and elevated lactic and hexanoic acid levels. ECG may reduce postmenopausal obesity by remodeling gut microbiota and reprogramming metabolism, indicating its potential as a functional food.

大柑橘外皮（ECG，花桔红），大柑橘的外皮，传统上用于化痰，也用于治疗痰湿型肥胖。需要进一步的证据来阐明其抗肥胖作用及其在肠道菌群调节中的作用。在本研究中，ECG显著降低了体重、血清TG、TC和LDL-c，而HDL-c升高（p < 0.05）。ingWAT、pgWAT和iBAT的代谢笼、qPCR和Western blot分析显示，能量消耗增加，产热基因（如Ucp1、Atp2a2）上调，脂质合成基因（如Acc、Fas）下调，脂溶和线粒体基因表达增强（如Atgl、Mgll）。它还通过降低TNF-α、IL-6和IL-1β，增加黏液层厚度，上调肠屏障基因（Zo-1, Occludin），显示出较强的抗炎作用。16S rDNA测序结果显示，β-多样性增加，scfa产生菌增多，乳酸和己酸水平升高。心电图可能通过重塑肠道菌群和重编程代谢来减少绝经后肥胖，表明其作为功能性食品的潜力。

{"title":"Exocarpium Citri Grandis alleviates postmenopausal obesity in ovariectomized mice through gut microbiota remodeling and metabolic reprogramming","authors":"Tingting Chen , Xiaoqin Wu , Like Xu , Jiaxiong Zhao , Weiru Liu , Jiangtao Zeng , Zhuohui Luo , Jiawen Huang","doi":"10.1016/j.jff.2025.107102","DOIUrl":"10.1016/j.jff.2025.107102","url":null,"abstract":"<div><div><em>Exocarpium Citri Grandis</em> (ECG, Huajuhong), the epicarp of <em>Citrus grandis</em> ‘Tomentosa’, traditionally used to resolve phlegm, is also applied against phlegm-dampness-type obesity. Further evidence is needed to clarify its anti-obesity effects and role in gut microbiota modulation. In this study, ECG significantly reduced body weight, serum TG, TC, and LDL-c, while HDL-c increased (<em>p</em> < 0.05). Metabolic cage, qPCR, and Western blot analyses of ingWAT, pgWAT, and iBAT showed increased energy expenditure, upregulated thermogenic genes (e.g., <em>Ucp1</em>, <em>Atp2a2</em>), downregulated lipid synthesis genes (e.g., <em>Acc</em>, <em>Fas</em>), and enhanced lipolytic and mitochondrial gene expression (e.g., <em>Atgl</em>, <em>Mgll</em>).It also showed strong anti-inflammatory effects by reducing TNF-α, IL-6, and IL-1β, increasing mucus layer thickness, and upregulating intestinal barrier genes (<em>Zo-1</em>, <em>Occludin</em>).16S rDNA sequencing revealed increased β-diversity, SCFA-producing bacteria, and elevated <em>lactic</em> and <em>hexanoic acid</em> levels. ECG may reduce postmenopausal obesity by remodeling gut microbiota and reprogramming metabolism, indicating its potential as a functional food.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"135 ","pages":"Article 107102"},"PeriodicalIF":4.0,"publicationDate":"2025-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145569128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polysaccharide from Ziziphus jujuba cv. Changzao prevented colitis in mice by regulating the inflammation-gut microbiota-metabolite axis 红枣多糖。肠早通过调节炎症-肠道微生物-代谢物轴预防小鼠结肠炎

IF 4 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Journal of Functional Foods

Pub Date : 2025-11-13 DOI: 10.1016/j.jff.2025.107098

Yina Huang , Yubin Chen , Jingyu He , Xunmei Shi , Yao He , Wu Sun , Chuanchao Wu , Kui Zhao , Di Xu

Polysaccharides derived from jujuba exhibit beneficial anti-ulcerative colitis effects, largely attributed to their potent antioxidant properties and other bioactivities. However, the mechanisms by which these polysaccharides from Ziziphus jujuba cv. Changzao modulate contribute to gut microbiota metabolism remain poorly understood. Therefore, this study aimed to instigate the effects of polysaccharides from Ziziphus jujuba cv. Changzao, sourced from various origins, on colitis. Our results demonstrated that LP-25 and FP-25 exhibited the most pronounced therapeutic efficacy, alleviating colitis symptoms and significantly reducing levels of pro-inflammation cytokines. Moreover, LP-25 and FP-25 promoted intestinal barrier repair by mitigating mucosal damage and upregulating the expression of ZO-1. Additionally, these polysaccharides favorably modulated gut microbiota composition-increasing the relative abundance of Firmicutes and Lactobacillus while decreasing that of Bacteroidetes. Furthermore, LP-25 and FP-25 may exert protective effects against colitis by regulating primary bile acid biosynthesis to protect colitis. Collectively, our findings suggest that LP-25 and FP-25 represent promising candidates for UC intervention, as their therapeutic effects are mediated through the regulation of gut microbiota and associated metabolic pathways.

从枣中提取的多糖显示出有益的抗溃疡性结肠炎作用，主要归因于其有效的抗氧化特性和其他生物活性。然而，从酸枣中提取多糖的机理尚不清楚。肠早调节对肠道菌群代谢的贡献尚不清楚。因此，本研究旨在探讨红枣多糖的抗氧化作用。长藻，多种来源，治疗结肠炎。我们的研究结果表明，LP-25和FP-25表现出最显著的治疗效果，减轻结肠炎症状，显著降低促炎症细胞因子水平。此外，LP-25和FP-25通过减轻黏膜损伤和上调ZO-1表达促进肠屏障修复。此外，这些多糖有利于调节肠道微生物群的组成——增加厚壁菌门和乳酸杆菌的相对丰度，同时减少拟杆菌门的相对丰度。此外，LP-25和FP-25可能通过调节初级胆汁酸的生物合成，对结肠炎起到保护作用。总的来说，我们的研究结果表明，LP-25和FP-25是UC干预的有希望的候选者，因为它们的治疗效果是通过调节肠道微生物群和相关的代谢途径介导的。

{"title":"Polysaccharide from Ziziphus jujuba cv. Changzao prevented colitis in mice by regulating the inflammation-gut microbiota-metabolite axis","authors":"Yina Huang , Yubin Chen , Jingyu He , Xunmei Shi , Yao He , Wu Sun , Chuanchao Wu , Kui Zhao , Di Xu","doi":"10.1016/j.jff.2025.107098","DOIUrl":"10.1016/j.jff.2025.107098","url":null,"abstract":"<div><div>Polysaccharides derived from jujuba exhibit beneficial anti-ulcerative colitis effects, largely attributed to their potent antioxidant properties and other bioactivities. However, the mechanisms by which these polysaccharides from <em>Ziziphus jujuba</em> cv. Changzao modulate contribute to gut microbiota metabolism remain poorly understood. Therefore, this study aimed to instigate the effects of polysaccharides from <em>Ziziphus jujuba</em> cv. Changzao, sourced from various origins, on colitis. Our results demonstrated that LP-25 and FP-25 exhibited the most pronounced therapeutic efficacy, alleviating colitis symptoms and significantly reducing levels of pro-inflammation cytokines. Moreover, LP-25 and FP-25 promoted intestinal barrier repair by mitigating mucosal damage and upregulating the expression of ZO-1. Additionally, these polysaccharides favorably modulated gut microbiota composition-increasing the relative abundance of Firmicutes and <em>Lactobacillus</em> while decreasing that of <em>Bacteroidetes</em>. Furthermore, LP-25 and FP-25 may exert protective effects against colitis by regulating primary bile acid biosynthesis to protect colitis. Collectively, our findings suggest that LP-25 and FP-25 represent promising candidates for UC intervention, as their therapeutic effects are mediated through the regulation of gut microbiota and associated metabolic pathways.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"135 ","pages":"Article 107098"},"PeriodicalIF":4.0,"publicationDate":"2025-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145517584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0