Pub Date : 2024-11-01DOI: 10.1016/j.jff.2024.106497
Qianqian Ouyang , Yuwei Jiang , Lifen Liu , Chengfeng Cai , Nandika Bandara , Puwang Li , Kefeng Wu , Hui Hong , Liang Chen
In order to prepare calcium supplements for efficient absorption and utilization in the human body, this study focused on synthesizing SNP-Ca by chelating Squarrosus nudus peptide (SNP) with calcium. We characterized its structure, stability, and calcium uptake properties in Caco-2 cells and its impact on osteogenic activity in vitro. Optimal preparation conditions were determined: a peptide-calcium mass ratio of 5:1, a 30-min reaction time, a temperature of 60 °C, and a pH of 7.0. Under these conditions, a calcium chelating rate of 68.32 % was achieved. Calcium binds to the peptide primarily via carboxyl oxygen and amino nitrogen atoms, and the morphology of SNP-Ca was similar to porous nanoflowers. Our cellular experiments revealed that SNP-Ca significantly increases calcium uptake in Caco-2 cells, stimulating proliferation, differentiation, and mineralization in MC3T3-E1 cells. Additionally, zebrafish larvae models showed enhanced bone formation following SNP-Ca administration. SNP-Ca has the potential of a novel calcium supplement.
{"title":"Preparation and characterization of Sipunculus nudus peptide-calcium chelate: Structural insights and osteogenic bioactivity assessment","authors":"Qianqian Ouyang , Yuwei Jiang , Lifen Liu , Chengfeng Cai , Nandika Bandara , Puwang Li , Kefeng Wu , Hui Hong , Liang Chen","doi":"10.1016/j.jff.2024.106497","DOIUrl":"10.1016/j.jff.2024.106497","url":null,"abstract":"<div><div>In order to prepare calcium supplements for efficient absorption and utilization in the human body, this study focused on synthesizing SNP-Ca by chelating <em>Squarrosus nudus</em> peptide (SNP) with calcium. We characterized its structure, stability, and calcium uptake properties in Caco-2 cells and its impact on osteogenic activity in vitro. Optimal preparation conditions were determined: a peptide-calcium mass ratio of 5:1, a 30-min reaction time, a temperature of 60 °C, and a pH of 7.0. Under these conditions, a calcium chelating rate of 68.32 % was achieved. Calcium binds to the peptide primarily via carboxyl oxygen and amino nitrogen atoms, and the morphology of SNP-Ca was similar to porous nanoflowers. Our cellular experiments revealed that SNP-Ca significantly increases calcium uptake in Caco-2 cells, stimulating proliferation, differentiation, and mineralization in MC3T3-E1 cells. Additionally, zebrafish larvae models showed enhanced bone formation following SNP-Ca administration. SNP-Ca has the potential of a novel calcium supplement.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"122 ","pages":"Article 106497"},"PeriodicalIF":3.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142656407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-01DOI: 10.1016/j.jff.2024.106534
Ahreum Kim , Yujin Kim , Jung Min Lee , Jin-Oh Chung , Jong Hwa Roh , Jung Ok Lee , Beom Joon Kim
AP collagen peptides (APCPs) are enzymatically decomposed collagen peptides that have been shown to promote hair growth and improve hair cuticle structure and thickness in mice. However, their impact on the elasticity and gloss of human hair has not been thoroughly investigated. Therefore, this study sought to identify the factors affecting hair elasticity and gloss in APCPs-treated human hair follicles (hHFs). Using Time-of-Flight Secondary Ion Mass Spectrometry (ToF-SIMS) analysis, changes in amino acid content related to hair elasticity and lipid components associated with hair gloss were measured. As a result, APCP treatment led to increases in proline and cysteine amino acid, as well as in lipid components such as 18-methyleicosanoic acid (18-MEA), lauric acid, oleic acid, 11,13-eicosenoic acid, behenic acid, methyleicosanoic acid and squalene. Fourier-transform infrared spectroscopy (FT-IR) analysis of hair cross-sections indicated an increase in the intensity of bonds such as S=O, C=O, cystine, C–N and C–H in APCPs-treated hHFs. Additionally, APCPs elevated the expression of Keratin 17, integral to the inner root sheaths (IRS) and outer root sheaths (ORS), as well as Keratins 31, 85, and 86, components of the hair cortex and medulla. APCPs treatment also resulted in increased bonded water and moisture content in hHFs, along with a reduction in cuticle surface roughness. These findings suggest that APCPs may have the potential to enhance hair elasticity and gloss.
{"title":"The impact of AP collagen peptides (APCPs) on hair shaft elasticity and gloss: A comprehensive analysis","authors":"Ahreum Kim , Yujin Kim , Jung Min Lee , Jin-Oh Chung , Jong Hwa Roh , Jung Ok Lee , Beom Joon Kim","doi":"10.1016/j.jff.2024.106534","DOIUrl":"10.1016/j.jff.2024.106534","url":null,"abstract":"<div><div>AP collagen peptides (APCPs) are enzymatically decomposed collagen peptides that have been shown to promote hair growth and improve hair cuticle structure and thickness in mice. However, their impact on the elasticity and gloss of human hair has not been thoroughly investigated. Therefore, this study sought to identify the factors affecting hair elasticity and gloss in APCPs-treated human hair follicles (hHFs). Using Time-of-Flight Secondary Ion Mass Spectrometry (ToF-SIMS) analysis, changes in amino acid content related to hair elasticity and lipid components associated with hair gloss were measured. As a result, APCP treatment led to increases in proline and cysteine amino acid, as well as in lipid components such as 18-methyleicosanoic acid (18-MEA), lauric acid, oleic acid, 11,13-eicosenoic acid, behenic acid, methyleicosanoic acid and squalene. Fourier-transform infrared spectroscopy (FT-IR) analysis of hair cross-sections indicated an increase in the intensity of bonds such as S=O, C=O, cystine, C–N and C–H in APCPs-treated hHFs. Additionally, APCPs elevated the expression of Keratin 17, integral to the inner root sheaths (IRS) and outer root sheaths (ORS), as well as Keratins 31, 85, and 86, components of the hair cortex and medulla. APCPs treatment also resulted in increased bonded water and moisture content in hHFs, along with a reduction in cuticle surface roughness. These findings suggest that APCPs may have the potential to enhance hair elasticity and gloss.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"122 ","pages":"Article 106534"},"PeriodicalIF":3.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142656474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-01DOI: 10.1016/j.jff.2024.106558
Xu Zhang , Yan Luo , Li Qin , Yage Ma , Dan Chen , Shenglan Zhao
Rich in flavonoids and phenolic compounds, walnut shells have lipid-lowering, antioxidant, and anti-inflammatory properties; however, their impact on blood lipids has not been widely documented. We investigated the effects of walnut shell extract (WSE) on the lipid metabolism and serum metabolomics of rats with obesity. The results showed that WSE reduced the body weight and serum TC、TG and LDL-c levels and increased the HDL-c levels in obese rats (p < 0.05). It also decreased the activities of liver enzymes (ALT and AST), and modulated the levels of major lipid metabolism markers, downregulating LEP and FAS and upregulating ADP, PPAR-γ, and HSL. Additionally, serum metabolomic profiling was used to identify 29 differential metabolites, four of which were most significantly correlated with glycolipid metabolism. These results suggest that the potential mechanism of action of WSE in the treatment of hyperlipidemia is related to its regulatory effects on glycolipid metabolic pathways.
{"title":"Effects of walnut shell extract on lipid metabolism and serum metabolomics in rats with high-fat diet-induced obesity","authors":"Xu Zhang , Yan Luo , Li Qin , Yage Ma , Dan Chen , Shenglan Zhao","doi":"10.1016/j.jff.2024.106558","DOIUrl":"10.1016/j.jff.2024.106558","url":null,"abstract":"<div><div>Rich in flavonoids and phenolic compounds, walnut shells have lipid-lowering, antioxidant, and anti-inflammatory properties; however, their impact on blood lipids has not been widely documented. We investigated the effects of walnut shell extract (WSE) on the lipid metabolism and serum metabolomics of rats with obesity. The results showed that WSE reduced the body weight and serum TC、TG and LDL-c levels and increased the HDL-c levels in obese rats (<em>p</em> < <em>0.05</em>). It also decreased the activities of liver enzymes (ALT and AST), and modulated the levels of major lipid metabolism markers, downregulating LEP and FAS and upregulating ADP, PPAR-γ, and HSL. Additionally, serum metabolomic profiling was used to identify 29 differential metabolites, four of which were most significantly correlated with glycolipid metabolism. These results suggest that the potential mechanism of action of WSE in the treatment of hyperlipidemia is related to its regulatory effects on glycolipid metabolic pathways.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"122 ","pages":"Article 106558"},"PeriodicalIF":3.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142656460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-01DOI: 10.1016/j.jff.2024.106506
Dongzhu Duan, Zhenzhen Du, Ling Zhao
The imbalance of gut microbiota ecology caused by alcohol consumption disrupts the gut-liver axis, leading to the occurrence and progression of alcoholic liver disease (ALD). This study investigates the effects of galangin (GA) on the gut-liver axis in an alcohol-induced mouse model of ALD, focusing on its potential to regulate gut microbiota composition, intestinal permeability, and hepatic inflammation. The results showed that GA intervention alleviated liver oxidative stress injury and inflammation, improved gut microbiota imbalance, regulated intestinal permeability by up-regulating the expression of tight junction proteins, and enhanced intestinal barrier function. GA treatment reduced liver oxidative stress and inflammation rectified, alcohol-induced dysbiosis, and bolstered the intestinal barrier by up-regulating tight junction proteins. Additionally, the improvement of ALD by GA is mainly related to the Metabolism of xenobiotics by cytochrome P450 and Glutathione metabolism pathways. These results suggest that GA may alleviate ALD by regulating abnormalities of the gut-liver axis.
{"title":"Galangin alleviates alcoholic liver disease by regulating the gut-liver axis","authors":"Dongzhu Duan, Zhenzhen Du, Ling Zhao","doi":"10.1016/j.jff.2024.106506","DOIUrl":"10.1016/j.jff.2024.106506","url":null,"abstract":"<div><div>The imbalance of gut microbiota ecology caused by alcohol consumption disrupts the gut-liver axis, leading to the occurrence and progression of alcoholic liver disease (ALD). This study investigates the effects of galangin (GA) on the gut-liver axis in an alcohol-induced mouse model of ALD, focusing on its potential to regulate gut microbiota composition, intestinal permeability, and hepatic inflammation. The results showed that GA intervention alleviated liver oxidative stress injury and inflammation, improved gut microbiota imbalance, regulated intestinal permeability by up-regulating the expression of tight junction proteins, and enhanced intestinal barrier function. GA treatment reduced liver oxidative stress and inflammation rectified, alcohol-induced dysbiosis, and bolstered the intestinal barrier by up-regulating tight junction proteins. Additionally, the improvement of ALD by GA is mainly related to the Metabolism of xenobiotics by cytochrome P450 and Glutathione metabolism pathways. These results suggest that GA may alleviate ALD by regulating abnormalities of the gut-liver axis.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"122 ","pages":"Article 106506"},"PeriodicalIF":3.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142552056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-01DOI: 10.1016/j.jff.2024.106545
Tehmina Bibi , Ammar B. Altemimi , Roshina Rabail , Seemal Munir , Muhammad Umar Shahbaz , Maryam Khalid Rizvi , Muhammad Faisal Manzoor , Gholamreza Abdi , Ahsan Ul Haq , Rana Muhammad Aadil
Metabolic syndrome (MetS) is one of the most significant worldwide public health issues. Hypertension, visceral obesity, hyperglycemia, dyslipidemia, and insulin resistance are some of the contributing variables. The latest MetS preventative measures have focused on lifestyle factors and combination therapy, including exercise and herbal treatment. Cinnamon (Cinnamomum verum) is rich in phytochemicals, including phenolic and volatile compounds, responsible for its antitumor, anticancer, antidiabetic, and anti-hypertriglyceridemia potentials. Numerous clinical studies have demonstrated the value of cinnamon’s anti-inflammatory properties in treating diabetes. Cinnamon is crucial in maintaining normal levels of lipids in the blood. In the past ten years, several clinical studies have examined the effectiveness of cinnamon administration in managing inflammation-related diseases, metabolic abnormalities, and polycystic ovarian syndrome. Comprehensive information about the chemical composition, nutritional compounds, phytoconstituents, and protective role of cinnamon and cinnamaldehyde against metabolic syndrome has been presented in this review. An effort has also been made to explain the mechanisms of blood glucose management, visceral obesity management, and cinnamon safety and toxicity aspects.
{"title":"The therapeutic perspective of cinnamon (Cinnamomum verum) consumption against metabolic syndrome","authors":"Tehmina Bibi , Ammar B. Altemimi , Roshina Rabail , Seemal Munir , Muhammad Umar Shahbaz , Maryam Khalid Rizvi , Muhammad Faisal Manzoor , Gholamreza Abdi , Ahsan Ul Haq , Rana Muhammad Aadil","doi":"10.1016/j.jff.2024.106545","DOIUrl":"10.1016/j.jff.2024.106545","url":null,"abstract":"<div><div>Metabolic syndrome (MetS)<!--> <!-->is one of the most significant worldwide public health issues. Hypertension, visceral obesity, hyperglycemia, dyslipidemia, and insulin resistance are some of the contributing variables. The latest MetS preventative measures have focused on lifestyle factors and combination therapy, including exercise and herbal treatment. Cinnamon <em>(Cinnamomum verum)</em> is rich in phytochemicals, including phenolic and volatile compounds, responsible for its antitumor, anticancer, antidiabetic, and anti-hypertriglyceridemia potentials. Numerous clinical studies have demonstrated the value of cinnamon’s anti-inflammatory properties in treating diabetes. Cinnamon is crucial in maintaining normal levels of lipids in the blood. In the past ten years, several clinical studies have examined the effectiveness of cinnamon administration in managing inflammation-related diseases,<!--> <!-->metabolic abnormalities, and polycystic ovarian syndrome. Comprehensive information about the chemical composition, nutritional compounds, phytoconstituents, and protective role of cinnamon and cinnamaldehyde against metabolic syndrome has been presented in this review. An effort has also been made to explain the mechanisms of blood glucose management, visceral obesity management, and cinnamon safety and toxicity aspects.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"122 ","pages":"Article 106545"},"PeriodicalIF":3.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142552061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-01DOI: 10.1016/j.jff.2024.106515
Wen Liu , Enqiang Li , Mingdong Hu
This study aimed to assess how effective walnut supplementation is in managing overweight and obesity. A thorough search of PubMed, Embase, and Cochrane Central Register of Controlled Trials was carried out until March 2024. Two reviewers independently examined the suitability of studies and assessed the quality of reporting in the randomized controlled trials (RCTs) that were included. The results indicated that adding walnuts to the diet significantly lowered total cholesterol (TC) levels (p < 0.0001) and low-density lipoprotein-cholesterol (LDL-C) levels (p < 0.001). However, there was no notable difference in weight loss (p > 0.05) and body mass index (BMI) (p > 0.05) between those who received walnut supplementation and the control groups. Based on the RCT data, it appears that walnut supplementation can effectively decrease TC and LDL-C levels. Additionally, it seems to be a safe choice for individuals who are overweight or obese, as it did not have an adverse effect on body weight.
{"title":"Efficacy of walnut supplementation in managing overweight and obesity: A meta-analysis of randomized clinical trials","authors":"Wen Liu , Enqiang Li , Mingdong Hu","doi":"10.1016/j.jff.2024.106515","DOIUrl":"10.1016/j.jff.2024.106515","url":null,"abstract":"<div><div>This study aimed to assess how effective walnut supplementation is in managing overweight and obesity. A thorough search of PubMed, Embase, and Cochrane Central Register of Controlled Trials was carried out until March 2024. Two reviewers independently examined the suitability of studies and assessed the quality of reporting in the randomized controlled trials (RCTs) that were included. The results indicated that adding walnuts to the diet significantly lowered total cholesterol (TC) levels (<em>p < 0.0001</em>) and low-density lipoprotein-cholesterol (LDL-C) levels (<em>p < 0.001</em>). However, there was no notable difference in weight loss (<em>p > 0.05</em>) and body mass index (BMI) (<em>p > 0.05</em>) between those who received walnut supplementation and the control groups. Based on the RCT data, it appears that walnut supplementation can effectively decrease TC and LDL-C levels. Additionally, it seems to be a safe choice for individuals who are overweight or obese, as it did not have an adverse effect on body weight.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"122 ","pages":"Article 106515"},"PeriodicalIF":3.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142561100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-01DOI: 10.1016/j.jff.2024.106560
Xiwei Liu , Yichao Ma , Jie Guo, Jun Wang
Chronic colonic inflammation might result in increased cell proliferation that poses risk of colitis-associated cancer(CAC), an aggressive subtype of colorectal cancer. This study aimed to investigate dietary intervention effect and mechanism of chitooligosaccharide(COS) on azoxymethane(AOM)/dextran sulfate sodium(DSS)-induced CAC development in mice. COS at dose of 500 mg/kg markedly suppressed colonic levels of tumor necrosis factor(TNF)-α, interleukin(IL)-1β, and IL-6 in CAC mice. Compared to CAC model controls, the number of colonic epithelial cells expressing Ki-67 and the colonic expression levels of cyclin D1 were decreased in COS-treated CAC mice. COS administration significantly down-regulated expression of micro(mi)RNA-155, toll-like receptor(TLR)4, nuclear factor-kappaB(NF-κB), phosphorylated signal transducer and activator of transcription protein 3(pSTAT3), and regenerating islet derived 3 gamma(Reg3g), whereas up-regulated the suppressors of cytokine signaling 1 (SOCS1) expression in CAC colons. Overall, COS exerted protective activity against AOM/DSS-induced colitis-associated carcinogenesis, mechanism of which was associated with its anti-proliferation effect possible via regulating miRNA-155/TLR4/Reg3g pathway.
{"title":"Anti-proliferation effect of chitooligosaccharide on colitis-associated cancer in mice: Possible involvement of miRNA-155/TLR4/Reg3g pathway","authors":"Xiwei Liu , Yichao Ma , Jie Guo, Jun Wang","doi":"10.1016/j.jff.2024.106560","DOIUrl":"10.1016/j.jff.2024.106560","url":null,"abstract":"<div><div>Chronic colonic inflammation might result in increased cell proliferation that poses risk of colitis-associated cancer(CAC), an aggressive subtype of colorectal cancer. This study aimed to investigate dietary intervention effect and mechanism of chitooligosaccharide(COS) on azoxymethane(AOM)/dextran sulfate sodium(DSS)-induced CAC development in mice. COS at dose of 500 mg/kg markedly suppressed colonic levels of tumor necrosis factor(TNF)-α, interleukin(IL)-1β, and IL-6 in CAC mice. Compared to CAC model controls, the number of colonic epithelial cells expressing Ki-67 and the colonic expression levels of cyclin D1 were decreased in COS-treated CAC mice. COS administration significantly down-regulated expression of micro(mi)RNA-155, toll-like receptor(TLR)4, nuclear factor-kappaB(NF-κB), phosphorylated signal transducer and activator of transcription protein 3(pSTAT3), and regenerating islet derived 3 gamma(Reg3g), whereas up-regulated the suppressors of cytokine signaling 1 (SOCS1) expression in CAC colons. Overall, COS exerted protective activity against AOM/DSS-induced colitis-associated carcinogenesis, mechanism of which was associated with its anti-proliferation effect possible <em>via</em> regulating miRNA-155/TLR4/Reg3g pathway.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"122 ","pages":"Article 106560"},"PeriodicalIF":3.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142656459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-01DOI: 10.1016/j.jff.2024.106553
Lili Li , Zhenghao Shi , Yuxuan Li , Jiacheng Xu , Shuchen Han , Jinyan Chen , Hanyang Xu , Jiemei Jiang , Yan Yang , Yongfang Gong
Salvianolic acid B (Sal B) is a natural compound extracted from the root of Salvia miltiorrhiza, which has multiple biological activities. Targeting Hippo pathway transcriptional coactivators YAP/TAZ combined with Smad3 is an effective strategy against renal fibrosis. However, whether Sal B improves DEN/CCl4/C2H5OH-induced renal fibrosis and regulates YAP/TAZ and Smad3 phospho-isoform, pSmad3C/pSmad3L is unknown. Presently, we used DEN/CCl4/C2H5OH to induce renal fibrosis in mice and TGF-β1 or MST1/2 inhibitor XMU-MP-1 to induce HK-2 cells to investigate the activity and molecular mechanisms of Sal B. The results demonstrated that Sal B ameliorated DEN/CCl4/C2H5OH-induced renal fibrosis, including reducing renal tissue injury, improving renal function, and decreasing collagen synthesis. Furthermore, Sal B downregulated YAP/TAZ and promoted their phosphorylation in vivo and in vitro. Moreover, Sal B upregulated pSmad3C but downregulated pSmad3L in vivo and in vitro. Collectively, this study revealed that Sal B may improve DEN/CCl4/C2H5OH-induced renal fibrosis by regulating YAP/TAZ and pSmad3C/pSmad3L.
{"title":"Salvianolic acid B improves DEN/CCl4/C2H5OH-induced renal fibrosis by regulating YAP/TAZ and pSmad3C/pSmad3L","authors":"Lili Li , Zhenghao Shi , Yuxuan Li , Jiacheng Xu , Shuchen Han , Jinyan Chen , Hanyang Xu , Jiemei Jiang , Yan Yang , Yongfang Gong","doi":"10.1016/j.jff.2024.106553","DOIUrl":"10.1016/j.jff.2024.106553","url":null,"abstract":"<div><div>Salvianolic acid B (Sal B) is a natural compound extracted from the root of <em>Salvia miltiorrhiza</em>, which has multiple biological activities. Targeting Hippo pathway transcriptional coactivators YAP/TAZ combined with Smad3 is an effective strategy against renal fibrosis. However, whether Sal B improves DEN/CCl<sub>4</sub>/C<sub>2</sub>H<sub>5</sub>OH-induced renal fibrosis and regulates YAP/TAZ and Smad3 phospho-isoform, pSmad3C/pSmad3L is unknown. Presently, we used DEN/CCl<sub>4</sub>/C<sub>2</sub>H<sub>5</sub>OH to induce renal fibrosis in mice and TGF-β1 or MST1/2 inhibitor XMU-MP-1 to induce HK-2 cells to investigate the activity and molecular mechanisms of Sal B. The results demonstrated that Sal B ameliorated DEN/CCl<sub>4</sub>/C<sub>2</sub>H<sub>5</sub>OH-induced renal fibrosis, including reducing renal tissue injury, improving renal function, and decreasing collagen synthesis. Furthermore, Sal B downregulated YAP/TAZ and promoted their phosphorylation <em>in vivo</em> and <em>in vitro.</em> Moreover, Sal B upregulated pSmad3C but downregulated pSmad3L <em>in vivo</em> and <em>in vitro</em>. Collectively, this study revealed that Sal B may improve DEN/CCl<sub>4</sub>/C<sub>2</sub>H<sub>5</sub>OH-induced renal fibrosis by regulating YAP/TAZ and pSmad3C/pSmad3L.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"122 ","pages":"Article 106553"},"PeriodicalIF":3.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142656473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-01DOI: 10.1016/j.jff.2024.106524
Yemei Dai , Yujing He , Yuan Ma, Xuan Yang, Yongli Huang, Hongmei Min, Xiaocui Liu
The in vitro research found that Auricularia auricula-judae (A. auricula) crude polysaccharides showed hypoglycemic and antioxidant properties, whereas purified polysaccharides were more effective. A. auricula polysaccharide was identified as a typical heteropolysaccharide, with a pyran-type sugar ring coupled via α- and β-type glycosidic linkage. The monosaccharide composition includes Man, Xyl, Ara, Glc, Rha, and galactose (Gal), at a molar ratio of 61.36:37.92:36.27:25.79:24.30:20.38, while also containing a small amount of galacturonic and glucuronic acid. A. auricula polysaccharide showed smooth, flaky structures, with a small portion exhibiting a filamentous and spherical morphology. A. auricula polysaccharides activate glycogen synthetase kinase 3β (GSK3β) phosphorylation, leading to increased hepatic glycogen production, and they reduce glucose synthesis and lower blood sugar levels by decreasing phosphoenolpyruvate carboxykinase (PEPCK) and glucose-6-phosphatase (G6Pase) enzyme activity, and enhancing FOXO1 phosphorylation. The findings suggested that A. auricula polysaccharides could be used as natural hypoglycemic agents.
{"title":"Purification, structural analysis, and hypoglycemic activity of Auricularia auricula-judae polysaccharides extracted with natural deep eutectic solvents","authors":"Yemei Dai , Yujing He , Yuan Ma, Xuan Yang, Yongli Huang, Hongmei Min, Xiaocui Liu","doi":"10.1016/j.jff.2024.106524","DOIUrl":"10.1016/j.jff.2024.106524","url":null,"abstract":"<div><div>The <em>in vitro</em> research found that <em>Auricularia auricula-judae (A. auricula</em>) crude polysaccharides showed hypoglycemic and antioxidant properties, whereas purified polysaccharides were more effective. <em>A. auricula</em> polysaccharide was identified as a typical heteropolysaccharide, with a pyran-type sugar ring coupled via α- and β-type glycosidic linkage. The monosaccharide composition includes Man, Xyl, Ara, Glc, Rha, and galactose (Gal), at a molar ratio of 61.36:37.92:36.27:25.79:24.30:20.38, while also containing a small amount of galacturonic and glucuronic acid. <em>A. auricula</em> polysaccharide showed smooth, flaky structures, with a small portion exhibiting a filamentous and spherical morphology. <em>A. auricula</em> polysaccharides activate glycogen synthetase kinase 3β (GSK3β) phosphorylation, leading to increased hepatic glycogen production, and they reduce glucose synthesis and lower blood sugar levels by decreasing phosphoenolpyruvate carboxykinase (PEPCK) and glucose-6-phosphatase (G6Pase) enzyme activity, and enhancing FOXO1 phosphorylation. The findings suggested that <em>A. auricula</em> polysaccharides could be used as natural hypoglycemic agents.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"122 ","pages":"Article 106524"},"PeriodicalIF":3.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142552057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-01DOI: 10.1016/j.jff.2024.106540
Suwimon Sutantawong , Bum Jin Kim , Russell F. Kuhfeld , Yunyao Qu , David C. Dallas
Proteolysis of whey proteins during gastrointestinal digestion releases bioactive peptides with potential health benefits. Our study examined whey-derived peptide release after digestion under static in vitro conditions and in three adults following whey protein isolate ingestion. Using LC-MS/MS-based peptidomics, we identified 1187 unique peptides in the in vitro gastric digesta, 449 in the in vitro intestinal digesta and 1041 in the human jejunum. Among these peptides, 61 were known to exert bioactivities including ACE inhibitory, antimicrobial, DPP-IV inhibitory, antioxidant, immunomodulatory, anticancer and opioid activities. The release of antimicrobial, antioxidant and opioid peptides suggests their potential role in promoting gut health. The peptide patterns produced across digestion in the in vitro model and in adult humans were strongly similar by amino acid frequency and moderately similar in terms of peptide abundances.
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