Journal of Functional Foods最新文献_第3页

Kaempferol protects against high-salt-induced hypertension and vascular endothelial injury by inhibiting ferroptosis through the ATF4/ACSL4 pathway

IF 3.8 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Journal of Functional Foods

Pub Date : 2025-02-01 DOI: 10.1016/j.jff.2025.106684

Qiuer Liang , Minghao Chen , Guangtian Chen , Pengli Xu , Lai Kwan Lam , Pengcheng Xie , Ting Xie , Wanqing Tu , Tianhao Liu , Xiaopeng Peng , Haoyao Yuan , Liguo Chen , Ya Xiao

To date, no specific treatments have been developed for high-salt-induced hypertensive vascular damage, emphasizing the need to elucidate its underlying molecular mechanisms for therapeutic innovation. Kaempferol (Kae), a natural flavonol belonging to the flavonoid family, has demonstrated significant vascular protective effects in previous studies. However, its impact on high-salt-induced hypertensive vascular injury remains unclear. This study aimed to explore the pharmacological effects and underlying mechanisms of Kae in mitigating hypertensive vascular damage. Using an 8 % high-salt diet model, we evaluated the effects of Kae (200 mg/kg/day) in wild-type (WT) and activating transcription factor 4 (ATF4^+/−) knockout mice. Additionally, human aortic endothelial cells (HAECs) cultured under high-salt conditions were used to assess Kae's protective effects, with ATF4 and acyl-CoA synthetase long-chain family member 4 (ACSL4) knocked down via siRNA transfection. Our results showed that Kae significantly reduced high-salt-induced hypertension, improved vascular endothelial dysfunction, inhibited ferroptosis, and suppressed ATF4 expression. Compared to ATF4^+/−mice on a high-salt diet, ATF4^+/−mice treated with Kae exhibited greater reductions in blood pressure and endothelial damage and more pronounced inhibition of ferroptosis. Surface plasmon resonance and molecular docking analyses revealed that Kae directly binds to ATF4, while proteome microarray and co-immunoprecipitation assays demonstrated that ATF4 interacts with ACSL4 in HAECs. Furthermore, inhibition of ATF4 or ACSL4 effectively alleviated endothelial ferroptosis induced by high-salt stress. In summary, our findings suggest that Kae mitigates high-salt-induced hypertension by inhibiting endothelial ferroptosis via the ATF4/ACSL4 pathway, providing a potential therapeutic strategy for hypertensive vascular injury.

{"title":"Kaempferol protects against high-salt-induced hypertension and vascular endothelial injury by inhibiting ferroptosis through the ATF4/ACSL4 pathway","authors":"Qiuer Liang , Minghao Chen , Guangtian Chen , Pengli Xu , Lai Kwan Lam , Pengcheng Xie , Ting Xie , Wanqing Tu , Tianhao Liu , Xiaopeng Peng , Haoyao Yuan , Liguo Chen , Ya Xiao","doi":"10.1016/j.jff.2025.106684","DOIUrl":"10.1016/j.jff.2025.106684","url":null,"abstract":"<div><div>To date, no specific treatments have been developed for high-salt-induced hypertensive vascular damage, emphasizing the need to elucidate its underlying molecular mechanisms for therapeutic innovation. Kaempferol (Kae), a natural flavonol belonging to the flavonoid family, has demonstrated significant vascular protective effects in previous studies. However, its impact on high-salt-induced hypertensive vascular injury remains unclear. This study aimed to explore the pharmacological effects and underlying mechanisms of Kae in mitigating hypertensive vascular damage. Using an 8 % high-salt diet model, we evaluated the effects of Kae (200 mg/kg/day) in wild-type (WT) and activating transcription factor 4 (ATF4<sup>+/−</sup>) knockout mice. Additionally, human aortic endothelial cells (HAECs) cultured under high-salt conditions were used to assess Kae's protective effects, with ATF4 and acyl-CoA synthetase long-chain family member 4 (ACSL4) knocked down via siRNA transfection. Our results showed that Kae significantly reduced high-salt-induced hypertension, improved vascular endothelial dysfunction, inhibited ferroptosis, and suppressed ATF4 expression. Compared to ATF4<sup>+/−</sup>mice on a high-salt diet, ATF4<sup>+/−</sup>mice treated with Kae exhibited greater reductions in blood pressure and endothelial damage and more pronounced inhibition of ferroptosis. Surface plasmon resonance and molecular docking analyses revealed that Kae directly binds to ATF4, while proteome microarray and co-immunoprecipitation assays demonstrated that ATF4 interacts with ACSL4 in HAECs. Furthermore, inhibition of ATF4 or ACSL4 effectively alleviated endothelial ferroptosis induced by high-salt stress. In summary, our findings suggest that Kae mitigates high-salt-induced hypertension by inhibiting endothelial ferroptosis via the ATF4/ACSL4 pathway, providing a potential therapeutic strategy for hypertensive vascular injury.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"125 ","pages":"Article 106684"},"PeriodicalIF":3.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143183059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of royal jelly consumption on clinical outcomes in patients with ischemic stroke: A triple-blind randomized controlled trial

IF 3.8 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Journal of Functional Foods

Pub Date : 2025-02-01 DOI: 10.1016/j.jff.2025.106688

Elham Karimi , Arman Arab , Fariborz Khorvash , Maryam Kazemi , Reza Amani

The therapeutic impact of royal jelly (RJ) consumption in patients with ischemic stroke is unknown. Therefore, we conducted a randomized, triple-blind, placebo-controlled clinical trial to evaluate the role of RJ supplementation on clinical and biochemical outcomes in patients recovering from ischemic stroke. Out of 64 enrolled patients (45–80 yrs.) with ischemic stroke, 32 were randomized to the RJ group and 32 to the placebo (control) group. Groups completed a 12-week intervention. The intervention group received 1000 mg/d of RJ dragee after breakfast. We evaluated stroke-related disability, quality of life, and inflammatory and oxidative stress markers at baseline and post-intervention. At post-intervention, serum levels of erythrocyte sedimentation rate decreased in the RJ group compared to the control group (adjusted mean difference, −8.65 mm/h [95 % CI, −14.75 to −2.55]). Additionally, serum nitric oxide levels increased in the RJ group (adjusted mean difference, 10.18 nmol/mL [95 % CI, 0.51 to 19.86]) post-intervention compared to the control group. Furthermore, the RJ group exhibited a decreased oxidative status index (adjusted mean difference, −0.003 [95 % CI, −0.006 to −0.0001]) and reduced odds of stroke-related disability (adjusted odds ratio, 0.20 [95 % CI, 0.05 to 0.70]) compared to the placebo group. Moreover, RJ supplementation improved the quality-of-life measures in the RJ group (adjusted mean difference, 16.64 [95 % CI, 1.17 to 32.12]) compared to the control group. Our findings reflect the potential benefits of RJ consumption on clinical and biochemical outcomes of patients recovering from ischemic stroke. Importantly, we acknowledge the necessity of additional studies to verify the efficacy of RJ supplementation in patients recovering from strokes.

Trial registration

Iranian Registry of Clinical Trials (IRCT20180818040827N4), registered on October 9, 2021; https://www.irct.ir/trial/59275

{"title":"Effects of royal jelly consumption on clinical outcomes in patients with ischemic stroke: A triple-blind randomized controlled trial","authors":"Elham Karimi , Arman Arab , Fariborz Khorvash , Maryam Kazemi , Reza Amani","doi":"10.1016/j.jff.2025.106688","DOIUrl":"10.1016/j.jff.2025.106688","url":null,"abstract":"<div><div>The therapeutic impact of royal jelly (RJ) consumption in patients with ischemic stroke is unknown. Therefore, we conducted a randomized, triple-blind, placebo-controlled clinical trial to evaluate the role of RJ supplementation on clinical and biochemical outcomes in patients recovering from ischemic stroke. Out of 64 enrolled patients (45–80 yrs.) with ischemic stroke, 32 were randomized to the RJ group and 32 to the placebo (control) group. Groups completed a 12-week intervention. The intervention group received 1000 mg/d of RJ dragee after breakfast. We evaluated stroke-related disability, quality of life, and inflammatory and oxidative stress markers at baseline and post-intervention. At post-intervention, serum levels of erythrocyte sedimentation rate decreased in the RJ group compared to the control group (adjusted mean difference, −8.65 mm/h [95 % CI, −14.75 to −2.55]). Additionally, serum nitric oxide levels increased in the RJ group (adjusted mean difference, 10.18 nmol/mL [95 % CI, 0.51 to 19.86]) post-intervention compared to the control group. Furthermore, the RJ group exhibited a decreased oxidative status index (adjusted mean difference, −0.003 [95 % CI, −0.006 to −0.0001]) and reduced odds of stroke-related disability (adjusted odds ratio, 0.20 [95 % CI, 0.05 to 0.70]) compared to the placebo group. Moreover, RJ supplementation improved the quality-of-life measures in the RJ group (adjusted mean difference, 16.64 [95 % CI, 1.17 to 32.12]) compared to the control group. Our findings reflect the potential benefits of RJ consumption on clinical and biochemical outcomes of patients recovering from ischemic stroke. Importantly, we acknowledge the necessity of additional studies to verify the efficacy of RJ supplementation in patients recovering from strokes.</div></div><div><h3>Trial registration</h3><div>Iranian Registry of Clinical Trials (IRCT20180818040827N4), registered on October 9, 2021; <span><span>https://www.irct.ir/trial/59275</span><svg><path></path></svg></span></div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"125 ","pages":"Article 106688"},"PeriodicalIF":3.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143182961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Weizmannia coagulans BC99 modulate gut microbiota after Helicobacter pylori eradication: A randomized double-blind placebo-controlled trial

IF 3.8 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Journal of Functional Foods

Pub Date : 2025-02-01 DOI: 10.1016/j.jff.2025.106681

Ying Wu , Shirui Zhai , Caie Wang , Zhouya Bai , Pingping Tian , Shanshan Tie , Yao Wang , Yingjian Zhang , Lina Zhao , Shaobin Gu

Probiotics interventions may contribute to eradicate Helicobacter pylori infection and reduce gastrointestinal symptom, but their mechanisms require more research. This study involved 100H. pylori-positive adults, randomly assigned to either Weizmannia coagulans BC99 group or Placebo group. Both groups were received bismuth quadruple therapy for 4 weeks and took either BC99 or placebo for 6 weeks. After 6 weeks, BC99 significantly reduced eradication rate and happening of adverse events. BC99 reduced GSRS score, particularly the severity of stomach-abdominal pain during hunger symptoms. Additionally, BC99 reduced the disruption of bismuth quadruple therapy on the gut microbiota, particularly significantly increasing the abundance of Prevotella, Bacillus, Candidatus_Saccharimonas and reducing the abundance of Lachnoclostridium, Intestinibacter, Holdemania, Desulfovibrio. These findings supported the use of W. coagulans BC99 in combination with BQT in the clinical management of patients with H. pylori infection.

{"title":"Weizmannia coagulans BC99 modulate gut microbiota after Helicobacter pylori eradication: A randomized double-blind placebo-controlled trial","authors":"Ying Wu , Shirui Zhai , Caie Wang , Zhouya Bai , Pingping Tian , Shanshan Tie , Yao Wang , Yingjian Zhang , Lina Zhao , Shaobin Gu","doi":"10.1016/j.jff.2025.106681","DOIUrl":"10.1016/j.jff.2025.106681","url":null,"abstract":"<div><div>Probiotics interventions may contribute to eradicate <em>Helicobacter pylori</em> infection and reduce gastrointestinal symptom, but their mechanisms require more research. This study involved 100<em>H. pylori</em>-positive adults, randomly assigned to either <em>Weizmannia coagulans</em> BC99 group or Placebo group. Both groups were received bismuth quadruple therapy for 4 weeks and took either BC99 or placebo for 6 weeks. After 6 weeks, BC99 significantly reduced eradication rate and happening of adverse events. BC99 reduced GSRS score, particularly the severity of stomach-abdominal pain during hunger symptoms. Additionally, BC99 reduced the disruption of bismuth quadruple therapy on the gut microbiota, particularly significantly increasing the abundance of <em>Prevotella</em>, <em>Bacillus</em>, <em>Candidatus_Saccharimonas</em> and reducing the abundance of <em>Lachnoclostridium</em>, <em>Intestinibacter</em>, <em>Holdemania</em>, <em>Desulfovibrio</em>. These findings supported the use of <em>W. coagulans</em> BC99 in combination with BQT in the clinical management of patients with <em>H. pylori</em> infection.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"125 ","pages":"Article 106681"},"PeriodicalIF":3.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143183056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of dietary protein hydrolysates on cognitive performance: A systematic review of randomized controlled trials involving adults

IF 3.8 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Journal of Functional Foods

Pub Date : 2025-02-01 DOI: 10.1016/j.jff.2025.106683

Micah S. Adams, Ronald P. Mensink, Peter J. Joris

Changes in cerebral blood flow (CBF) - a marker of brain vascular function - may improve cognitive performance, and enhancing CBF through a healthy lifestyle could mitigate cognitive decline. Protein hydrolysates have shown potential to improve brain health. This systematic review analyzed randomized controlled trials on the effects of protein hydrolysates on cognitive performance and CBF in healthy adults and specific target populations. 4747 studies were identified, yielding 13 papers investigating cognitive performance, with four of those also evaluating CBF. Two egg-protein hydrolysates out of five studies on inhibitory control reported cognitive performance improvements. Three out of six studies from various protein sources observed enhancements in visuospatial memory. A link between CBF and cognitive performance was not observed. In conclusion, some studies have indicated that certain protein hydrolysates may benefit cognitive performance, but further research is needed to clarify contradictory findings and to identify beneficial bioactive peptides.

{"title":"Impact of dietary protein hydrolysates on cognitive performance: A systematic review of randomized controlled trials involving adults","authors":"Micah S. Adams, Ronald P. Mensink, Peter J. Joris","doi":"10.1016/j.jff.2025.106683","DOIUrl":"10.1016/j.jff.2025.106683","url":null,"abstract":"<div><div>Changes in cerebral blood flow (CBF) - a marker of brain vascular function - may improve cognitive performance, and enhancing CBF through a healthy lifestyle could mitigate cognitive decline. Protein hydrolysates have shown potential to improve brain health. This systematic review analyzed randomized controlled trials on the effects of protein hydrolysates on cognitive performance and CBF in healthy adults and specific target populations. 4747 studies were identified, yielding 13 papers investigating cognitive performance, with four of those also evaluating CBF. Two egg-protein hydrolysates out of five studies on inhibitory control reported cognitive performance improvements. Three out of six studies from various protein sources observed enhancements in visuospatial memory. A link between CBF and cognitive performance was not observed. In conclusion, some studies have indicated that certain protein hydrolysates may benefit cognitive performance, but further research is needed to clarify contradictory findings and to identify beneficial bioactive peptides.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"125 ","pages":"Article 106683"},"PeriodicalIF":3.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143183671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multi-trace elements-enriched functional drink accelerates gastric ulcer repair via the HGF/c-Met/STAT3 pathway

IF 3.8 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Journal of Functional Foods

Pub Date : 2025-02-01 DOI: 10.1016/j.jff.2025.106674

Yongnan Piao , Nuoya Wang , Mingji Jin , Jianyu Piao , Mingfeng Han , Zifei Wang , Chunhua Quan , Jishan Yin , Zhonggao Gao , Wenxiang Cui , Shuangqing Wang , Xiuquan Quan

Heavy alcohol consumption can lead to various gastrointestinal disorders, with gastric ulcers being the most prevalent. In this study, we explored the reparative effects of a functional drink (FD) enriched with trace elements (TEs) on ethanol-induced gastric ulcers in rats and elucidated its underlying mechanism. Rats were administered anhydrous ethanol (5 mL/kg) orally to induce gastric ulcers and were then randomly assigned to a model, an omeprazole (OME, 20 mg/kg), FD with low-dose (FD-L), medium-dose (FD-M), high-dose (FD-H), FD without added TEs (FD without TEs), and an omeprazole+low-dose group (OME + L). FD increased the activities of antioxidant factors (T-SOD, GSH, CAT, and IL-10), and decreased the inflammatory factors levels (TNF-α, IL-6, MDA, COX-2, and iNOS) by promoting the HGF/c-Met/STAT3 pathway. It also effectively regulate the abundance and diversity of intestinal flora. Finally, FD reduced the severity of gastric mucosal injury caused by invasive factors, improved the morphological structure of gastric mucosal cells, enhanced the ability of oxidative stress, and inhibited excessive inflammatory response. In addition, the combination of OME and FD-L did not significantly affect the therapeutic efficacy of OME, but reduced its adverse effects. Ultimately, FD presentation had a pro-healing effect on the ethanol-induced gastric ulcer model. In conclusion, FD has a wide range of developmental prospects and offers an attractive approach and protocol for the treatment of clinical gastric ulcers.

{"title":"Multi-trace elements-enriched functional drink accelerates gastric ulcer repair via the HGF/c-Met/STAT3 pathway","authors":"Yongnan Piao , Nuoya Wang , Mingji Jin , Jianyu Piao , Mingfeng Han , Zifei Wang , Chunhua Quan , Jishan Yin , Zhonggao Gao , Wenxiang Cui , Shuangqing Wang , Xiuquan Quan","doi":"10.1016/j.jff.2025.106674","DOIUrl":"10.1016/j.jff.2025.106674","url":null,"abstract":"<div><div>Heavy alcohol consumption can lead to various gastrointestinal disorders, with gastric ulcers being the most prevalent. In this study, we explored the reparative effects of a functional drink (FD) enriched with trace elements (TEs) on ethanol-induced gastric ulcers in rats and elucidated its underlying mechanism. Rats were administered anhydrous ethanol (5 mL/kg) orally to induce gastric ulcers and were then randomly assigned to a model, an omeprazole (OME, 20 mg/kg), FD with low-dose (FD-L), medium-dose (FD-M), high-dose (FD-H), FD without added TEs (FD without TEs), and an omeprazole+low-dose group (OME + L). FD increased the activities of antioxidant factors (T-SOD, GSH, CAT, and IL-10), and decreased the inflammatory factors levels (TNF-α, IL-6, MDA, COX-2, and iNOS) by promoting the HGF/c-Met/STAT3 pathway. It also effectively regulate the abundance and diversity of intestinal flora. Finally, FD reduced the severity of gastric mucosal injury caused by invasive factors, improved the morphological structure of gastric mucosal cells, enhanced the ability of oxidative stress, and inhibited excessive inflammatory response. In addition, the combination of OME and FD-L did not significantly affect the therapeutic efficacy of OME, but reduced its adverse effects. Ultimately, FD presentation had a pro-healing effect on the ethanol-induced gastric ulcer model. In conclusion, FD has a wide range of developmental prospects and offers an attractive approach and protocol for the treatment of clinical gastric ulcers.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"125 ","pages":"Article 106674"},"PeriodicalIF":3.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143183052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Asparagus officinalis polysaccharide-loaded liposomal gels alleviate skin inflammation via inhibiting the NF-κB/MAPK pathways

IF 3.8 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Journal of Functional Foods

Pub Date : 2025-02-01 DOI: 10.1016/j.jff.2025.106679

Rongrui Zhang , Yu Xiong , Yujin Ma , Yafei Ma , Nuo Li , Yan Ren , Lili He , Lu Han , Jun Ni , Zhi-xiang Yuan

Asparagus officinalis L. has long been used as a medicinal food since ancient times. Despite identifying the active compounds, the anti-inflammatory properties of these compounds remain underexplored. This study explored the anti-inflammatory activity of white asparagus polysaccharide (WAPS) against skin inflammation. WAPS, a pectin polysaccharide, was identified to coexist with α-glycosidic and β-glycosidic linkages glucan and probably has triple-helix confirmation suggested by Fourier-transform infrared and nuclear magnetic resonance spectroscopy. Cellular experiments indicated that WAPS attenuated the expression of inflammatory cytokines and enhanced macrophage viability. Skin inflammation was ameliorated with WAPS liposomal-loaded gel; the mechanism regulating NF-κB/MAPK signal pathways was further explored. Notably, the formulation augmented the bioavailability of WAPS, downregulated the expression of NF-κB p50 and MMP9, and amplified the inhibitory potential of WAPS against inflammation. This study suggests WAPS as a medicinal substance with anti-inflammatory efficacy.

{"title":"Asparagus officinalis polysaccharide-loaded liposomal gels alleviate skin inflammation via inhibiting the NF-κB/MAPK pathways","authors":"Rongrui Zhang , Yu Xiong , Yujin Ma , Yafei Ma , Nuo Li , Yan Ren , Lili He , Lu Han , Jun Ni , Zhi-xiang Yuan","doi":"10.1016/j.jff.2025.106679","DOIUrl":"10.1016/j.jff.2025.106679","url":null,"abstract":"<div><div><em>Asparagus officinalis</em> L. has long been used as a medicinal food since ancient times. Despite identifying the active compounds, the anti-inflammatory properties of these compounds remain underexplored. This study explored the anti-inflammatory activity of white asparagus polysaccharide (WAPS) against skin inflammation. WAPS, a pectin polysaccharide, was identified to coexist with <em>α</em>-glycosidic and <em>β</em>-glycosidic linkages glucan and probably has triple-helix confirmation suggested by Fourier-transform infrared and nuclear magnetic resonance spectroscopy. Cellular experiments indicated that WAPS attenuated the expression of inflammatory cytokines and enhanced macrophage viability. Skin inflammation was ameliorated with WAPS liposomal-loaded gel; the mechanism regulating NF-κB/MAPK signal pathways was further explored. Notably, the formulation augmented the bioavailability of WAPS, downregulated the expression of NF-κB p50 and MMP9, and amplified the inhibitory potential of WAPS against inflammation. This study suggests WAPS as a medicinal substance with anti-inflammatory efficacy.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"125 ","pages":"Article 106679"},"PeriodicalIF":3.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143183053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Levying evidence of the impact of Triphala in the mildly constipated human colon microbiota

IF 3.8 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Journal of Functional Foods

Pub Date : 2025-02-01 DOI: 10.1016/j.jff.2025.106698

Elizabeth Goya-Jorge , Pauline Bondue , Irma Gonza , Samiha Boutaleb , Caroline Douny , Marie-Louise Scippo , Joël Pincemail , Patrice Chiap , Jeoffrey Christyn de Ribaucourt , Fabienne Crahay , Véronique Delcenserie

Triphala is a wildly used nutritional and phytotherapeutic formulation combining three dry fruits (Terminalia bellirica, Terminalia chebula and Emblica officinalis). Despite its long-standing use to treat gastrointestinal discomfort, there is limited understanding of Triphala's effects on gut microbiota, especially among individuals with mild constipation. Thus, this study aimed to investigate Triphala's impact on the constipated human colon microbiota. A short-term (72 h) static configuration of the in vitro Simulator of the Human Intestinal Microbial Ecosystem (SHIME®) system was used to study the fermentation process of a standardized extract of Triphala in the gut microbiota. Chromatographic and enzymatic methods were used to analyze the microbe-derived metabolic production. Potential herb-human host interactions were assessed using in vitro cell-based methods. Triphala extract increased Akkermansia muciniphila but decreased Bifidobacterium spp. in the simulated colon microbiota. Metabolic profiling of Triphala treatment showed increased phenolic species and antioxidant potential and reduced ammonia, valeric, isovaleric, and isobutyric acids during fermentation, potentially benefiting intestinal health, especially in contexts of constipation. Fermentation metabolites enhanced transepithelial electrical resistance in a human epithelium model and inhibited aryl hydrocarbon receptor (AhR) transcriptional activity. Triphala's polyphenols likely cause this AhR antagonism. Overall, these findings state some potential explanations for the usefulness of Triphala as a natural remedy for gastrointestinal diseases. However, it also raises concerns about some harmful effects of Triphala in the gut microbial ecosystem of people suffering from mild constipation.

Triphala 是一种广泛使用的营养和植物治疗配方，由三种干果（Terminalia bellirica、Terminalia chebula 和 Emblica officinalis）组合而成。尽管 Triphala 长期用于治疗肠胃不适，但人们对其对肠道微生物群的影响了解有限，尤其是对轻度便秘患者的影响。因此，本研究旨在调查 Triphala 对便秘患者结肠微生物群的影响。本研究采用人体肠道微生物生态系统体外模拟器（SHIME®）系统的短期（72 小时）静态配置，研究了标准化的 Triphala 提取物在肠道微生物群中的发酵过程。色谱法和酶法用于分析微生物产生的代谢产物。使用体外细胞方法评估了潜在的草药与人类宿主之间的相互作用。在模拟结肠微生物群中，Triphala 提取物增加了 Akkermansia muciniphila，但减少了双歧杆菌。三叶草提取物的代谢分析表明，发酵过程中酚类物质和抗氧化潜力增加，氨、戊酸、异戊酸和异丁酸减少，这可能有益于肠道健康，尤其是在便秘的情况下。在人体上皮细胞模型中，发酵代谢物增强了经上皮细胞电阻，并抑制了芳基烃受体（AhR）的转录活性。Triphala 的多酚可能会导致这种 AhR 拮抗作用。总之，这些发现为三叶草作为治疗胃肠道疾病的天然药物提供了一些可能的解释。不过，这些发现也引发了人们对三叶草对轻度便秘患者肠道微生物生态系统有害影响的担忧。

{"title":"Levying evidence of the impact of Triphala in the mildly constipated human colon microbiota","authors":"Elizabeth Goya-Jorge , Pauline Bondue , Irma Gonza , Samiha Boutaleb , Caroline Douny , Marie-Louise Scippo , Joël Pincemail , Patrice Chiap , Jeoffrey Christyn de Ribaucourt , Fabienne Crahay , Véronique Delcenserie","doi":"10.1016/j.jff.2025.106698","DOIUrl":"10.1016/j.jff.2025.106698","url":null,"abstract":"<div><div>Triphala is a wildly used nutritional and phytotherapeutic formulation combining three dry fruits (<em>Terminalia bellirica</em>, <em>Terminalia chebula</em> and <em>Emblica officinalis</em>). Despite its long-standing use to treat gastrointestinal discomfort, there is limited understanding of Triphala's effects on gut microbiota, especially among individuals with mild constipation. Thus, this study aimed to investigate Triphala's impact on the constipated human colon microbiota. A short-term (72 h) static configuration of the <em>in vitro</em> Simulator of the Human Intestinal Microbial Ecosystem (SHIME®) system was used to study the fermentation process of a standardized extract of Triphala in the gut microbiota. Chromatographic and enzymatic methods were used to analyze the microbe-derived metabolic production. Potential herb-human host interactions were assessed using <em>in vitro</em> cell-based methods. Triphala extract increased <em>Akkermansia muciniphila</em> but decreased <em>Bifidobacterium spp.</em> in the simulated colon microbiota. Metabolic profiling of Triphala treatment showed increased phenolic species and antioxidant potential and reduced ammonia, valeric, isovaleric, and isobutyric acids during fermentation, potentially benefiting intestinal health, especially in contexts of constipation. Fermentation metabolites enhanced transepithelial electrical resistance in a human epithelium model and inhibited aryl hydrocarbon receptor (AhR) transcriptional activity. Triphala's polyphenols likely cause this AhR antagonism. Overall, these findings state some potential explanations for the usefulness of Triphala as a natural remedy for gastrointestinal diseases. However, it also raises concerns about some harmful effects of Triphala in the gut microbial ecosystem of people suffering from mild constipation.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"125 ","pages":"Article 106698"},"PeriodicalIF":3.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143182951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Goji berry (Lycium barbarum) has positive effects on the mucosa and colonic myoenteric innervation of obese rats

IF 3.8 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Journal of Functional Foods

Pub Date : 2025-02-01 DOI: 10.1016/j.jff.2025.106663

Samara Cristina Dossena , Isabela Ramos Mariano Furlan , Maria Montserrat Diaz Pedrosa , Rosângela Fernandes Garcia , Fernando Carlos de Sousa , Maria Raquel Marçal Natali

Goji berry (Lycium barbarum), characterized as a functional food, has been shown to be effective in the prevention and/or treatment of obesity. This study investigated the effects of Goji berry supplementation (250 mg/kg) on the intestine of male Wistar rats, from 70 to 130 days (group OGB) or 21 to 130 days (group OPR) of age, with obesity induced by simple carbohydrate-rich diet. The diet did not change the morphology or the thickness of the proximal colon wall but increased the proportion of small neurons in the myoenteric plexus. The use of the Goji berry extract had therapeutic effect on the obese animals (group OGB), decreasing body mass and mesenteric fat and increasing HDL cholesterol. As a preventive agent (group OPR) it reduced the body mass index, preserved the percentage of neutral mucins-producing goblet cells and increased the incidence of large neurons in the colonic myoenteric plexus. The use of the Goji berry extract is a promising alternative for the preservation and restoration of the intestinal structure and organization compromised by an obesogenic diet.

枸杞子（枸杞）是一种功能性食品，已被证明可有效预防和/或治疗肥胖症。本研究调查了枸杞子补充剂（250 毫克/千克）对雄性 Wistar 大鼠肠道的影响，这些大鼠年龄为 70 至 130 天（OGB 组）或 21 至 130 天（OPR 组），由富含简单碳水化合物的饮食诱发肥胖。饮食没有改变近端结肠壁的形态或厚度，但增加了肠肌丛中小神经元的比例。枸杞提取物对肥胖动物（OGB 组）有治疗作用，可降低体重和肠系膜脂肪，增加高密度脂蛋白胆固醇。作为一种预防剂（OPR 组），枸杞提取物降低了体重指数，保持了产生中性粘蛋白的小肠细胞的比例，并增加了结肠肌肠神经丛中大神经元的发生率。使用枸杞提取物是保护和恢复因肥胖饮食而受损的肠道结构和组织的一种有前途的替代方法。

{"title":"Goji berry (Lycium barbarum) has positive effects on the mucosa and colonic myoenteric innervation of obese rats","authors":"Samara Cristina Dossena , Isabela Ramos Mariano Furlan , Maria Montserrat Diaz Pedrosa , Rosângela Fernandes Garcia , Fernando Carlos de Sousa , Maria Raquel Marçal Natali","doi":"10.1016/j.jff.2025.106663","DOIUrl":"10.1016/j.jff.2025.106663","url":null,"abstract":"<div><div>Goji berry (<em>Lycium barbarum</em>), characterized as a functional food, has been shown to be effective in the prevention and/or treatment of obesity. This study investigated the effects of Goji berry supplementation (250 mg/kg) on the intestine of male Wistar rats, from 70 to 130 days (group OGB) or 21 to 130 days (group OPR) of age, with obesity induced by simple carbohydrate-rich diet. The diet did not change the morphology or the thickness of the proximal colon wall but increased the proportion of small neurons in the myoenteric plexus. The use of the Goji berry extract had therapeutic effect on the obese animals (group OGB), decreasing body mass and mesenteric fat and increasing HDL cholesterol. As a preventive agent (group OPR) it reduced the body mass index, preserved the percentage of neutral mucins-producing goblet cells and increased the incidence of large neurons in the colonic myoenteric plexus. The use of the Goji berry extract is a promising alternative for the preservation and restoration of the intestinal structure and organization compromised by an obesogenic diet.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"125 ","pages":"Article 106663"},"PeriodicalIF":3.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143183689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Screen of propionate-producing probiotic strains in attenuating murine colitis by modulating immune response and restoring the intestinal barrier 通过调节免疫反应和恢复肠道屏障减轻小鼠结肠炎的丙酸盐益生菌菌株筛选

IF 3.8 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Journal of Functional Foods

Pub Date : 2025-02-01 DOI: 10.1016/j.jff.2025.106660

Ting Shu , Xizi Song , Silu Zhang , Xiaoli Zhou , Zhizhu Zhang , Pengfei Wang , Hu Canying , Jie Pan , Xiaoshuang Dai , Yan Tan , Wu Xiyang , Huicui Meng , Guoxun Xiao , Huaijie Hao

Intestinal dysbiosis is central to inflammatory bowel disease (IBD) development. Probiotics produce propionate, a short-chain fatty acid that restores intestinal balance and reduces inflammation.

Comparative genomic analysis of propionate pathway integrity was performed to nominate 41 candidate strains. Metabolic analysis confirmed the propionate production of 9 strains of them. Caco-2/HT29-MTX-E12 confluence epithelial model was established to investigate the effect of the strains on intestinal cell. DSS-induced colitis in a mouse model was also included as an in vivo verification of the filtered probiotic strains.

The methods successfully nominated 2 potential strains: Bifidobacterium breve XA-2103 and Pediococcus acidilactici GOLDGUT-PA0755. Both had a preferential ability to produce propionate and to reduce the effect on the intestinal barrier in the confluence cell model. Furthermore, their therapeutic effects were verified by reduced body weight loss, shortening of the colon length, Disease Activity Index score and improvement in histological analysis.

{"title":"Screen of propionate-producing probiotic strains in attenuating murine colitis by modulating immune response and restoring the intestinal barrier","authors":"Ting Shu , Xizi Song , Silu Zhang , Xiaoli Zhou , Zhizhu Zhang , Pengfei Wang , Hu Canying , Jie Pan , Xiaoshuang Dai , Yan Tan , Wu Xiyang , Huicui Meng , Guoxun Xiao , Huaijie Hao","doi":"10.1016/j.jff.2025.106660","DOIUrl":"10.1016/j.jff.2025.106660","url":null,"abstract":"<div><div>Intestinal dysbiosis is central to inflammatory bowel disease (IBD) development. Probiotics produce propionate, a short-chain fatty acid that restores intestinal balance and reduces inflammation.</div><div>Comparative genomic analysis of propionate pathway integrity was performed to nominate 41 candidate strains. Metabolic analysis confirmed the propionate production of 9 strains of them. Caco-2/HT29-MTX-E12 confluence epithelial model was established to investigate the effect of the strains on intestinal cell. DSS-induced colitis in a mouse model was also included as an in vivo verification of the filtered probiotic strains.</div><div>The methods successfully nominated 2 potential strains: <em>Bifidobacterium breve</em> XA-2103 and <em>Pediococcus acidilactici</em> GOLDGUT-PA0755. Both had a preferential ability to produce propionate and to reduce the effect on the intestinal barrier in the confluence cell model. Furthermore, their therapeutic effects were verified by reduced body weight loss, shortening of the colon length, Disease Activity Index score and improvement in histological analysis.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"125 ","pages":"Article 106660"},"PeriodicalIF":3.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143183049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sargassum fusiforme and its fucoidan alleviates high-fat diet induced obesity associated with the improvement of the gut microbiota profile 马尾藻及其褐藻糖胶减轻高脂饮食引起的肥胖症，与肠道微生物群特征的改善有关

IF 3.8 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Journal of Functional Foods

Pub Date : 2025-02-01 DOI: 10.1016/j.jff.2025.106686

Xiaoyu Ling, Zhengyang Li, Ning Su, Lingqing Kong, Shiwei Han, Dafeng Song

Obesity is considered a significant global health issue. The gut microbiota plays a crucial role in preventing obesity. Sargassum fusiforme and its fucoidan extract have lipid-lowering effects, but their mechanisms are not yet fully understood. This study investigates the mechanisms by exploring changes in gut microbiota composition, gene expression, and metabolites in mice fed a high-fat diet after intervention with Sargassum fusiforme (SF) and Sargassum fusiforme fucoidan (SFF) through integration of 16S rRNA sequencing, transcriptomics, and non-targeted metabolomics. Our results demonstrate that Sargassum fusiforme can ameliorate high-fat diet-induced obesity by decreasing the abundance of specific Firmicutes phylum bacteria, reducing the levels of glycochenodeoxycholic acid, glycoursodeoxycholic acid, glycohyodeoxycholic acid, and glycodeoxycholic acid, and regulating the expression of the Elovl3, Gm3734, Per2, and Tnnc1 genes. This offers a new perspective for hypercholesterolemia treatment strategies.

肥胖症被认为是一个重大的全球健康问题。肠道微生物群在预防肥胖方面发挥着至关重要的作用。马尾藻及其褐藻糖胶提取物具有降脂作用，但其作用机制尚未完全明了。本研究通过整合 16S rRNA 测序、转录组学和非靶向代谢组学，探索马尾藻（SF）和马尾藻褐藻糖胶（SFF）干预高脂饮食后小鼠肠道微生物群组成、基因表达和代谢物的变化，从而研究其机制。我们的研究结果表明，马尾藻褐藻糖胶能通过减少特定固醇菌门细菌的丰度，降低糖脱氧胆酸、糖酪脱氧胆酸、糖羟脱氧胆酸和糖脱氧胆酸的水平，以及调节Elovl3、Gm3734、Per2和Tnnc1基因的表达，来改善高脂饮食引起的肥胖。这为高胆固醇血症的治疗策略提供了新的视角。

{"title":"Sargassum fusiforme and its fucoidan alleviates high-fat diet induced obesity associated with the improvement of the gut microbiota profile","authors":"Xiaoyu Ling, Zhengyang Li, Ning Su, Lingqing Kong, Shiwei Han, Dafeng Song","doi":"10.1016/j.jff.2025.106686","DOIUrl":"10.1016/j.jff.2025.106686","url":null,"abstract":"<div><div>Obesity is considered a significant global health issue. The gut microbiota plays a crucial role in preventing obesity. <em>Sargassum fusiforme</em> and its fucoidan extract have lipid-lowering effects, but their mechanisms are not yet fully understood. This study investigates the mechanisms by exploring changes in gut microbiota composition, gene expression, and metabolites in mice fed a high-fat diet after intervention with <em>Sargassum fusiforme</em> (SF) and <em>Sargassum fusiforme</em> fucoidan (SFF) through integration of 16S rRNA sequencing, transcriptomics, and non-targeted metabolomics. Our results demonstrate that <em>Sargassum fusiforme</em> can ameliorate high-fat diet-induced obesity by decreasing the abundance of specific Firmicutes phylum bacteria, reducing the levels of glycochenodeoxycholic acid, glycoursodeoxycholic acid, glycohyodeoxycholic acid, and glycodeoxycholic acid, and regulating the expression of the Elovl3, Gm3734, Per2, and Tnnc1 genes. This offers a new perspective for hypercholesterolemia treatment strategies.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"125 ","pages":"Article 106686"},"PeriodicalIF":3.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143182952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0