Journal of Functional Foods最新文献_第5页

The role of a high-salt diet in the modulation of silicosis 高盐饮食在矽肺调节中的作用

IF 4 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Journal of Functional Foods

Pub Date : 2026-02-01 Epub Date: 2026-01-07 DOI: 10.1016/j.jff.2025.107141

Jing Wu , Yimin Shi , Yanyan Ke , Yaping Xu , Ta-Hui Lin , Jie Wang , Huaxiu Sui , Xue Yi

Silicosis remains a significant occupational health threat worldwide, affecting millions of workers in various industries. To better understand its underlying mechanisms and identify new intervention targets, the current study explores the pathogenesis of pneumoconiosis. Osteoclast stimulatory transmembrane protein (OC-STAMP) has been found to be an important marker for SiO₂-induced activation and phenotypic transformation of alveolar type II epithelial cells(ACE2). Moreover, the interaction between OC-STAMP and Moesin is important for regulating epithelial-mesenchymal transition (EMT). Recently, it has been found that a high-salt diet (HSD) may enhance antitumor immune responses and inhibit tumor EMT, achieving antitumor effects. To investigate the role of an HSD in silicosis fibrosis, we intervened with a diet containing 4 % NaCl in a rat model of silicosis to study its effects. The experimental results showed that the HSD slowed the progression of silicosis fibrosis. It was able to downregulate the expression of OC-STAMP, thereby inhibiting the ferroptosis of ACE2, which contributed to the attenuation of lung fibrosis. Furthermore, in in vitro studies, SiO₂ induced ACE2 to produce excessive ROS, Fe²⁺, MDA, and HYP, which could be corrected by a HSD. In a SiO₂-induced fibrosis model, high salt reverses ferroptosis and the fibrosis phenotype. To explore the underlying mechanisms, we screened for the OC-STAMP potential interacting protein Moesin, which acts together with OC-STAMP to regulate downstream ferroptosis. High salt downregulates OC-STAMP levels to inhibit fibrosis, which is involved in ferroptosis. In conclusion, these results provide evidence that high salt inhibits ferroptosis in epithelial cells and delays lung fibrosis by regulating OC-STAMP/Moesin interactions, offering insights into the pathogenesis of silicosis.

矽肺病仍然是世界范围内一个重大的职业健康威胁，影响着各行各业的数百万工人。为了更好地了解其潜在机制并确定新的干预靶点，本研究对尘肺病的发病机制进行了探讨。破骨细胞刺激跨膜蛋白（OC-STAMP）已被发现是sio2诱导肺泡II型上皮细胞（ACE2）活化和表型转化的重要标志物。此外，OC-STAMP和Moesin之间的相互作用对于调节上皮-间质转化（EMT）很重要。近年来，研究发现高盐饮食可增强抗肿瘤免疫反应，抑制肿瘤EMT，达到抗肿瘤的作用。为了研究HSD在矽肺纤维化中的作用，我们在矽肺大鼠模型中干预了含有4% NaCl的饮食，以研究其作用。实验结果表明，HSD能减缓矽肺纤维化的进展。它能够下调OC-STAMP的表达，从而抑制ACE2的铁下垂，从而促进肺纤维化的衰减。此外，在体外研究中，SiO2诱导ACE2产生过量的ROS、Fe2+、MDA和HYP，这可以通过HSD来纠正。在二氧化硅诱导的纤维化模型中，高盐可逆转铁下垂和纤维化表型。为了探索潜在的机制，我们筛选了OC-STAMP潜在的相互作用蛋白Moesin，该蛋白与OC-STAMP一起调节下游铁凋亡。高盐可下调OC-STAMP水平以抑制纤维化，这与铁下垂有关。总之，这些结果提供了高盐通过调节OC-STAMP/Moesin相互作用抑制上皮细胞铁下垂并延缓肺纤维化的证据，为矽肺的发病机制提供了新的见解。

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引用次数: 0

Beta vulgaris L. extract and betanin alleviate dextran sulfate sodium (DSS)-induced colitis in mice by enhancing the intestinal barrier and regulating the gut microbiota to suppress inflammation 甜菜素和甜菜素可通过增强肠道屏障和调节肠道菌群抑制炎症来缓解DSS诱导的小鼠结肠炎

IF 4 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Journal of Functional Foods

Pub Date : 2026-02-01 Epub Date: 2026-01-28 DOI: 10.1016/j.jff.2026.107179

Gwang-Pyo Ko , Hyejun Jo , Jungman Kim , Minseong Park , Mingun Kim , Kyung-Hwan Boo , Chang Sook Kim

The gut microbiota and intestinal barrier are essential for intestinal homeostasis, and their disruption contributes to ulcerative colitis (UC). This study evaluated the protective effects of red beetroot extract (RE) and its major pigment, betanin (BN), against dextran sulfate sodium (DSS)-induced UC in mice. Male C57BL/6 J mice were assigned to Health, DSS, DSS + low-dose RE (LRE, 300 mg/kg), DSS + high-dose RE (HRE, 900 mg/kg), and DSS + BN (50 mg/kg) groups. LRE showed no meaningful protective effects, whereas HRE significantly alleviated DSS-induced body weight loss, colon shortening, mucus layer disruption, and tight junction damage (occludin and claudin-1). BN also improved these outcomes, exhibiting efficacy comparable to HRE, although HRE showed slightly greater improvements across several colitis-related symptoms. Both HRE and BN inhibited NF-κB activation, reduced pro-inflammatory cytokines, and improved microbial balance by enhancing α-diversity, enriching short-chain fatty acid (SCFA)–producing bacteria, and suppressing pathogens. Functional prediction (PICRUSt2) indicated recovery of inflammation-related pathways. Overall, HRE and BN mitigated colitis by reinforcing the gut barrier, suppressing inflammation, and modulating the gut microbiota.

肠道菌群和肠道屏障对肠道稳态至关重要，它们的破坏有助于溃疡性结肠炎（UC）。本研究评价了红甜菜根提取物（RE）及其主要色素甜菜素（BN）对硫酸葡聚糖钠（DSS）诱导的小鼠UC的保护作用。雄性C57BL/ 6j小鼠分为健康组、DSS组、DSS +低剂量RE组（LRE, 300 mg/kg）、DSS +高剂量RE组（HRE, 900 mg/kg）和DSS + BN组（50 mg/kg）。LRE无明显保护作用，而HRE可显著缓解dss诱导的体重减轻、结肠缩短、黏液层破坏和紧密连接损伤（occludin和claudin-1）。BN也改善了这些结果，显示出与HRE相当的疗效，尽管HRE在几种结肠炎相关症状上表现出略大的改善。HRE和BN均可抑制NF-κB活化，降低促炎因子，通过增强α-多样性、丰富短链脂肪酸（SCFA）产生菌、抑制病原菌等途径改善微生物平衡。功能预测（PICRUSt2）显示炎症相关通路恢复。总的来说，HRE和BN通过加强肠道屏障、抑制炎症和调节肠道微生物群来减轻结肠炎。

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引用次数: 0

Extraction, purification, structural characterization, and bioactivities of Asparagus polysaccharides 芦笋多糖的提取、纯化、结构表征及生物活性研究

IF 4 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Journal of Functional Foods

Pub Date : 2026-02-01 Epub Date: 2026-01-07 DOI: 10.1016/j.jff.2026.107153

Yutong Chen , Lijie Wang , Weichen Xia , Yiwen Tao , Shuangfeng Xiong , Xinge Lan , Yuxin He , Ling Li , Gang Fan

The genus Asparagus, comprising numerous species, holds significant value as a source of food, medicinal compounds and ornamental plants. Among the plants within this genus, Asparagus cochinchinensis (Lour.) Merr. and Asparagus officinalis L., as classic species within the genus Asparagus, have been extensively studied for their nutraceutical and therapeutic applications. It is well known that these plants contain a diverse array of bioactive compounds, among which polysaccharides have emerged as high-value functional constituents due to their well-documented immunomodulatory, antitumor, and antioxidant effects. The current state of research on Asparagus polysaccharides has led to the establishment of standardized extraction methods, including hot-water, ultrasound-assisted, and enzyme-assisted techniques. However, there is currently a lack of systematic summary and comparison of the extraction methods for Asparagus polysaccharides. This deficiency likely hinders a deep understanding of their structure-activity relationships and further impedes the elucidation of the underlying pharmacological mechanisms. Thus, this review provides a comprehensive survey of research progress in Asparagus polysaccharides, encompassing extraction and purification methods, structural feature analysis, biological activity evaluation, and application development. The integration of extant preparation techniques, structural data, and pharmacology datasets facilitates the systematic exploration of structure-activity relationships, thereby supporting their therapeutic applications.

芦笋属，包括许多种类，具有重要价值的食物来源，药用化合物和观赏植物。在该属植物中，胭脂芦笋（Lour.）稳定。芦笋（Asparagus officinalis L.）作为芦笋属的经典种，因其营养和治疗用途而被广泛研究。众所周知，这些植物含有多种生物活性化合物，其中多糖因其充分证明的免疫调节、抗肿瘤和抗氧化作用而成为高价值的功能成分。目前对芦笋多糖的研究已经建立了标准化的提取方法，包括热水法、超声辅助法和酶辅助法。然而，目前对芦笋多糖的提取方法缺乏系统的总结和比较。这种缺陷可能阻碍了对其结构-活性关系的深入理解，并进一步阻碍了对潜在药理机制的阐明。本文就芦笋多糖的提取纯化方法、结构特征分析、生物活性评价和应用开发等方面的研究进展作一综述。现有制备技术、结构数据和药理学数据集的整合促进了结构-活性关系的系统探索，从而支持其治疗应用。

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引用次数: 0

Yogurt fermented with Lactobacillus delbrueckii ssp. bulgaricus OLL1073R-1 and Streptococcus thermophilus OLS3059 enhances dendritic cell activity and reduces cold-like symptoms in healthy adults: A randomized controlled trial 用德氏乳杆菌汤发酵的酸奶。保加利亚链球菌ol1073r -1和嗜热链球菌OLS3059增强健康成人树突状细胞活性并减轻感冒样症状：一项随机对照试验

IF 4 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Journal of Functional Foods

Pub Date : 2026-02-01 Epub Date: 2026-01-09 DOI: 10.1016/j.jff.2026.107154

Shuyi Tang , Takehiro Yokoo , Takayuki Toshimitsu , Toshihiro Sashihara , Yoshitaka Iwama , Satoshi Hachimura , Kenichi Hojo

The immunomodulatory effects of yogurt fermented with Lactobacillus delbrueckii ssp. bulgaricus OLL1073R-1 and Streptococcus thermophilus OLS3059 were evaluated in a randomized, double-blind, placebo-controlled trial involving 140 healthy males and females. The participants ingested test yogurt or placebo daily for eight weeks. The yogurt group exhibited significantly increased CD86⁺ and CD80⁺ conventional dendritic cell (cDC) populations, CD40⁺ plasmacytoid DC (pDC) populations upon viral antigen stimulation, CD69 expression on CD4⁺ T cells, and salivary s-IgA levels compared to those in the placebo group. The onset of local (nausea) and systemic (fatigue, general malaise, and feeling somewhat unwell) cold-like symptoms was significantly reduced in the yogurt group compared to that in the placebo group. These results suggest that intake of yogurt fermented with OLL1073R-1 and OLS3059 enhances cDC and pDC activities and subsequently promotes CD4⁺ T cell activation and salivary s-IgA production, thereby contributing to health maintenance.

德氏乳杆菌发酵酸奶的免疫调节作用。在一项涉及140名健康男性和女性的随机、双盲、安慰剂对照试验中，对保加利亚链球菌ol1073r -1和嗜热链球菌OLS3059进行了评估。参与者每天摄入测试酸奶或安慰剂，持续八周。与安慰剂组相比，酸奶组在病毒抗原刺激下表现出CD86+和CD80+常规树突状细胞（cDC）群、CD40+浆细胞样DC （pDC）群、CD4+ T细胞上CD69表达和唾液s-IgA水平的显著增加。与安慰剂组相比，酸奶组的局部（恶心）和全身性（疲劳、全身不适和感觉有些不适）感冒样症状的发作明显减少。综上所述，摄入含有OLL1073R-1和OLS3059发酵的酸奶可提高cDC和pDC活性，进而促进CD4+ T细胞活化和唾液s-IgA的产生，从而有助于健康维持。

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引用次数: 0

A polysaccharide from Artocarpus heterophyllus Lam. pulp ameliorates oxidative stress and lipid accumulation in HepG2 cells 一种从石竹中提取的多糖。牙髓改善HepG2细胞的氧化应激和脂质积累

IF 4 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Journal of Functional Foods

Pub Date : 2026-02-01 Epub Date: 2026-01-14 DOI: 10.1016/j.jff.2026.107163

Panjie Xu , Benyan Bai , Huawei Chen , Yanjun Zhang , Zhiyuan Ma , Gang Wu , Yan Zhou , Kexue Zhu

Oxidative stress and hepatic lipid accumulation are two key contributors to the development of non-alcoholic fatty liver disease (NAFLD). The present research sought to examine the effect of a polysaccharide from Artocarpus heterophyllus Lam. pulp (JFP-Ps) on alleviating hydrogen peroxide (H₂O₂)-induced cellular oxidative stress, and attenuating oleic acid (OA)-induced lipid accumulation in HepG2 cells, respectively. Results showed that JFP-Ps considerably lower intracellular ROS and MDA concentrations, elevate the activities of CAT, SOD, GSH-Px, and T-AOC. Furthermore, JFP-Ps exhibited antioxidant effects by up-regulating Nrf2, NQO1 and HO-1 expression, and down-regulating ERK and P38 expression in H₂O₂-treated HepG2 cells. Western blot determination indicated that JFP-Ps up-regulated the expression of Nrf2/HO-1/NQO1 in the Nrf2 pathway and down-regulated the expression of PERK and p-P38 in the MAPK pathway. JFP-Ps decreased the TG and TC contents, reduced the relative area of lipid droplets, and restored OA-elicited lipid metabolism disorders in HepG2 cells. The lipid-lowering impact of JFP-Ps is facilitated through down-regulating PPARγ/SREBP1/HMGCR/FAS, and upregulating of CPT1A/CYP7A1 expressions. These results could offer valuable insights for the potential application of JFP-Ps in NAFLD treatment.

氧化应激和肝脏脂质积累是非酒精性脂肪性肝病（NAFLD）发展的两个关键因素。摘要本研究旨在探讨一种从石竹中提取的多糖的作用。JFP-Ps分别减轻过氧化氢（H2O2）诱导的细胞氧化应激和油酸（OA）诱导的HepG2细胞脂质积累。结果表明，JFP-Ps显著降低了细胞内ROS和MDA浓度，提高了CAT、SOD、GSH-Px和T-AOC的活性。此外，JFP-Ps通过上调h2o2处理的HepG2细胞中Nrf2、NQO1和HO-1的表达，下调ERK和P38的表达，表现出抗氧化作用。Western blot检测显示，JFP-Ps上调Nrf2通路中Nrf2/HO-1/NQO1的表达，下调MAPK通路中PERK和p-P38的表达。JFP-Ps降低了TG和TC含量，减少了脂滴的相对面积，恢复了oa引起的HepG2细胞脂质代谢紊乱。JFP-Ps的降脂作用是通过下调PPARγ/SREBP1/HMGCR/FAS，上调CPT1A/CYP7A1表达来实现的。这些结果为JFP-Ps在NAFLD治疗中的潜在应用提供了有价值的见解。

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引用次数: 0

Erythritol as a functional food ingredient mitigates heat-induced renal injury in mice via integrated metabolomic and transcriptomic analysis 通过综合代谢组学和转录组学分析，赤藓糖醇作为功能性食品成分减轻了小鼠热致肾损伤

IF 4 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Journal of Functional Foods

Pub Date : 2026-02-01 Epub Date: 2026-01-10 DOI: 10.1016/j.jff.2026.107157

Haohong Zheng , Jialiang Xin , Xiaofang Yan , Jiali Wang , Yunjiang Liu , Cao Deng , Zhijun Zhong , Haifeng Liu , Ziyao Zhou , Guangneng Peng

Global warming increases the risk of heat-related renal impairment, while higher ambient temperatures may increase overall fluid and beverage consumption to meet hydration demands. As erythritol is widely used as a dietary sweetener in food and beverage products and is primarily excreted via the kidneys, concerns have been raised regarding its renal effects, particularly under conditions of heat stress. Here, we used integrated metabolomic and transcriptomic analyses to explore erythritol's role in heat-induced renal injury in mice. Erythritol supplementation modulated oxidative stress, inflammation, and lipid metabolism, with metabolomics showing recovery of redox-related metabolites and transcriptomics revealing downregulation of pro-inflammatory genes (e.g., Cxcl9, Serpina3g) and stress chaperones, alongside upregulation of antioxidative genes (e.g., Gbp6, Tgtp1). Multi-omics highlighted PPAR and AMPK pathways as key targets. Animal experiments confirmed improved renal histology, reduced creatinine, and decreased oxidative and inflammatory markers. These findings indicate that erythritol protects against heat-induced renal damage, supporting its potential as a functional food ingredient for thermally stressful conditions.

全球变暖增加了与热有关的肾脏损害的风险，而较高的环境温度可能会增加总体液体和饮料的消耗，以满足水合需求。由于赤藓糖醇被广泛用作食品和饮料产品中的膳食甜味剂，并且主要通过肾脏排泄，因此人们对其对肾脏的影响提出了担忧，特别是在热应激条件下。在这里，我们使用综合代谢组学和转录组学分析来探索赤藓糖醇在小鼠热致肾损伤中的作用。红糖醇的补充调节了氧化应激、炎症和脂质代谢，代谢组学显示氧化还原相关代谢物的恢复，转录组学显示促炎基因（如Cxcl9、Serpina3g）和应激伴侣的下调，以及抗氧化基因（如Gbp6、Tgtp1）的上调。多组学强调PPAR和AMPK通路是关键靶点。动物实验证实肾脏组织学改善，肌酐降低，氧化和炎症标志物降低。这些发现表明赤藓糖醇可以防止热引起的肾脏损伤，支持其作为热应激条件下的功能性食品成分的潜力。

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引用次数: 0

Digestates of pea and fava bean proteins induce FGF19 in human intestinal HT29 cells: Evidence for a potential role of PPARγ 豌豆和蚕豆蛋白的消化物在人肠道HT29细胞中诱导FGF19: PPARγ潜在作用的证据

IF 4 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Journal of Functional Foods

Pub Date : 2026-02-01 Epub Date: 2026-01-28 DOI: 10.1016/j.jff.2026.107175

Bérengère Benoit , Clémence Defois-Fraysse , Audrey Jalabert , Sandra Pesenti , Lexane Brunet , Alice Beau , Emmanuelle Loizon , Claudie Pinteur , Nadia Bendridi , Margaux Nawrot , Murielle Godet , Jennifer Rieusset , Marie-Caroline Michalski , Fabrice Desailly , Quentin Bailleul , Caroline Perreau , Catherine Lefranc-Millot , Hubert Vidal

Plant-based proteins are promising alternatives to animal proteins for supporting muscle synthesis and preventing sarcopenia. This study examined whether protein digestates (PDs) from pea and fava bean isolates modulate intestinal Fibroblast Growth Factor 19 (FGF19), a hormone central to energy and muscle mass regulation. Human HT29 cells were exposed to PDs, GW4064 (FXR agonist), or rosiglitazone (PPARγ agonist). FGF19 secretion was assessed by ELISA, gene expression by RT-qPCR, and FXR protein by Western-blot. At 6 h, PDs alone had no effect on FGF19 expression but potentiated GW4064 activity by lowering its EC₅₀. At 24 h, PDs modestly increased FGF19 and continued to potentiate GW4064 activity. Rosiglitazone + GW4064 markedly increased FGF19 and FXR expression, an effect abolished by GW9662 (PPARγ inhibitor), confirming PPARγ involvement. GW9662 also suppressed FGF19 induction by PDs + GW4064. Thus, PPARγ may regulate the FXR–FGF19 axis in enterocytes. Both PDs and rosiglitazone enhance FXR-mediated FGF19, with PDs partly acting through PPARγ. Pea and fava bean proteins may offer nutritional strategies for metabolic health and sarcopenia prevention.

植物蛋白是支持肌肉合成和预防肌肉减少症的动物蛋白的有希望的替代品。本研究考察了豌豆和蚕豆分离物的蛋白质消化物（pd）是否调节肠成纤维细胞生长因子19 (FGF19)，这是一种对能量和肌肉质量调节至关重要的激素。人HT29细胞暴露于pd、GW4064 （FXR激动剂）或罗格列酮（PPARγ激动剂）。ELISA检测FGF19分泌，RT-qPCR检测FGF19基因表达，Western-blot检测FXR蛋白表达。在6小时，单独的pd对FGF19表达没有影响，但通过降低其EC₅0来增强GW4064的活性。24 h时，pd适度增加FGF19，并继续增强GW4064的活性。罗格列酮+ GW4064显著增加FGF19和FXR的表达，GW9662 （PPARγ抑制剂）消除了这一作用，证实了PPARγ参与。GW9662也能抑制pd + GW4064诱导的FGF19。因此，PPARγ可能调节肠细胞中的FXR-FGF19轴。pd和罗格列酮均可增强fxr介导的FGF19，其中pd部分通过PPARγ起作用。豌豆和蚕豆蛋白可能为代谢健康和预防肌肉减少症提供营养策略。

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引用次数: 0

Protective role of egg white protein hydrolysate against streptozotocin-induced hepatotoxicity in rats 蛋清蛋白水解物对链脲佐菌素所致大鼠肝毒性的保护作用

IF 4 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Journal of Functional Foods

Pub Date : 2026-02-01 Epub Date: 2026-01-07 DOI: 10.1016/j.jff.2026.107151

Rungrudee Srisawat , Naiyana Nontamart , Kusuma Ruamthum , Pongsak Cherngkhuntod , Parin Suwannaprapha , Ratsa Sripirom , Supawadee Khamphuech , Suphaket Seanthaweesuk , Nuntiya Somparn , Jirawat Yongsawatdigul , Atcharaporn Thaeomor

Chronic hyperglycemia in diabetes contributes to oxidative stress, which plays a critical role in liver injury. This study evaluated the hepatoprotective potential of egg white protein hydrolysate (EWH) in streptozotocin (STZ)-induced diabetic rats. Male Wistar rats, diabetic and non-diabetic, received oral water, metformin (200 mg/kg), or EWH (300, 600, or 1200 mg/kg) for four weeks. STZ-induced diabetes significantly increased serum liver enzymes, total bilirubin, and hepatic malondialdehyde (MDA), while decreasing the activities of hepatic antioxidant enzymes (superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx)). EWH treatment, particularly at 600 mg/kg, significantly decreased liver enzymes and MDA levels and restored antioxidant enzyme activities. Histological analysis revealed that EWH markedly improved liver architecture by reducing hepatocellular degeneration, inflammation, and bile duct proliferation. These results suggest that EWH has hepatoprotective effects, likely due to its antioxidant bioactive peptides, and may serve as a potential therapeutic agent against liver damage in diabetes.

糖尿病慢性高血糖导致氧化应激，在肝损伤中起关键作用。本研究评价了蛋清蛋白水解物（EWH）对链脲佐菌素（STZ）诱导的糖尿病大鼠的肝保护作用。雄性Wistar大鼠，糖尿病和非糖尿病，给予口服水、二甲双胍（200 mg/kg）或EWH(300、600或1200 mg/kg) 4周。stz诱导的糖尿病显著升高血清肝酶、总胆红素和肝丙二醛（MDA），降低肝脏抗氧化酶（超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GPx））活性。EWH处理（特别是600 mg/kg）显著降低了肝酶和MDA水平，恢复了抗氧化酶活性。组织学分析显示，EWH通过减少肝细胞变性、炎症和胆管增殖显著改善肝脏结构。这些结果表明，EWH具有肝脏保护作用，可能是由于其抗氧化生物活性肽，并且可能作为一种潜在的治疗糖尿病肝损伤的药物。

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引用次数: 0

Rice bran fermented by Lactococcus lactis subsp. lactis MJM60392 reduces fat accumulation and adipose tissue on high-fat diet induced obese mice 乳球菌发酵米糠的研究。lactis MJM60392减少高脂饮食诱导的肥胖小鼠的脂肪堆积和脂肪组织

IF 4 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Journal of Functional Foods

Pub Date : 2026-02-01 Epub Date: 2026-01-28 DOI: 10.1016/j.jff.2026.107178

Hak Yong Lee , Young Mi Park , Dong Yeop Shin , Hai Min Hwang , Sung Hak Chun , Sang Jin Lim , Myung-Sunny Kim , Min-Jung Kim , Hye-Jeong Yang , Yong Hee Jung , Jun Sang Bae , Jae Gon Kim

Obesity, which can develop owing to a high-calorie diet and a sedentary lifestyle, may increase the risk of developing diabetes, hyperlipidemia, and cardiovascular diseases. Therefore, management through exercise and dietary control is essential. However, supplementation may be necessary if exercise and dietary management are difficult. This study aimed to evaluate materials that could potentially prevent obesity. The research investigated whether the intake of rice bran fermented by Lactococcus lactis subsp. lactis MJM60392 (FRBL) would affect body fat reduction in a high-fat diet (HFD)-induced obesity model. During the intake period, a reduction in body weight was observed in FRBL fed- compared with that in only HFD fed-obese mice. After autopsy, a decrease in liver and visceral fat weights was noted, along with a reduction in levels of ALP and leptin, which were elevated in the obesity model. Histological analysis revealed a reduction in fat accumulation in the liver due to FRBL and a decrease in adipocyte size in the epididymal fat tissue. Finally, the expression of genes related to fat synthesis and differentiation was also found to be reduced. In conclusion, FRBL intake was found to help reduce body fat by inhibiting fat synthesis and differentiation, thereby suppressing the accumulation.

由于高热量饮食和久坐不动的生活方式，肥胖可能会增加患糖尿病、高脂血症和心血管疾病的风险。因此，通过锻炼和饮食控制进行管理是必不可少的。然而，如果运动和饮食管理有困难，补充剂可能是必要的。这项研究旨在评估可能预防肥胖的材料。本研究考察了乳球菌发酵米糠的摄取量。lactis MJM60392 （FRBL）会影响高脂饮食（HFD）诱导的肥胖模型的体脂减少。在摄入期间，与仅饲喂HFD的肥胖小鼠相比，饲喂FRBL的小鼠体重有所减轻。尸检后，发现肝脏和内脏脂肪重量减少，ALP和瘦素水平降低，而肥胖模型中ALP和瘦素水平升高。组织学分析显示，由于FRBL，肝脏中的脂肪堆积减少，附睾脂肪组织中的脂肪细胞大小减少。最后，与脂肪合成和分化相关的基因表达也被发现减少。综上所述，摄入FRBL可以通过抑制脂肪的合成和分化，从而抑制脂肪的积累，从而帮助减少体脂。

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引用次数: 0

Anti-inflammatory effect of Cyanidin-3-O-lathyroside from Eleutherococcus sessiliflorus in LPS-induced RAW 264.7 cells 无梗棘球绦虫花青素-3- o -腺苷对lps诱导的RAW 264.7细胞的抗炎作用

IF 4 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Journal of Functional Foods

Pub Date : 2026-02-01 Epub Date: 2026-01-19 DOI: 10.1016/j.jff.2026.107169

Amylin Jay Eunice Cabuloy , Seo-Jin Lee , Don Carlo Batara , Heon-Woong Kim , Sung-Hak Kim , Jeong-Yong Cho

The anti-inflammatory effect of cyanidin-3-O-lathyroside was investigated in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages by examining cell morphology and quantifying pro-inflammatory cytokine expression by qPCR. Extracellular cytokine levels and the activity of inflammatory mediators were measured using ELISA and nitric oxide (NO) assays. Immunoblotting of the nuclear factor-kappa B (NF-κB) pathway targets and immunofluorescent staining of p65 were performed to elucidate the inhibitory mechanism. Treatment with cyanidin-3-O-lathyroside consistently reduced macrophage activation, as well as the mRNA and protein expression of cytokines (including tumor necrosis factor alpha (TNF-α), interleukin-1 beta (IL-1β), and interleukin-6 (IL-6)) and inflammatory mediators such as inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2). The phosphorylation of IκB-α and the nuclear translocation of p65 were also effectively inhibited by cyanidin-3-O-lathyroside at higher concentrations. Overall, the results demonstrate a dose-dependent suppression of LPS-induced inflammation in RAW 264.7 cells, supporting the potential of cyanidin-3-O-lathyroside as a natural anti-inflammatory agent.

本研究通过检测脂多糖（LPS）刺激下的RAW 264.7巨噬细胞的细胞形态和促炎细胞因子的定量表达，探讨了花青素-3- o-腺苷的抗炎作用。采用酶联免疫吸附试验和一氧化氮（NO）测定细胞外细胞因子水平和炎症介质活性。通过核因子κB （NF-κB）通路靶点的免疫印迹和p65的免疫荧光染色来阐明其抑制机制。用花青素-3- o -甲状腺苷治疗可持续降低巨噬细胞的活化，以及细胞因子(包括肿瘤坏死因子α (TNF-α)、白细胞介素-1β （IL-1β)和白细胞介素-6 (IL-6)）和炎症介质（如诱导型一氧化氮合酶（iNOS）和环氧化酶-2 (COX-2)） mRNA和蛋白的表达。高浓度的花青素-3- o -甲状腺苷也能有效抑制IκB-α的磷酸化和p65的核易位。总体而言，结果表明lps对RAW 264.7细胞诱导的炎症具有剂量依赖性抑制作用，支持了花青素-3- o - lagthyroside作为天然抗炎剂的潜力。

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