Pub Date : 2024-12-01DOI: 10.1016/j.jff.2024.106624
Yuyang Yao , Juer Liu , Qiming Miao , Xinyue Zhu , Lu Sun , Wei Hua , Na Zhang , Guangwei Huang , Roger Ruan , Yanling Cheng , Shengquan Mi
Controlling postprandial hyperglycemia is considered as a viable prophylactic treatment for type 2 diabetes mellitus. A major strategy in decreasing postprandial glucose involves inhibition of the carbohydrate-hydrolyzing enzymes, α-amylase and α-glucosidase. The aim of this study was to evaluate the hypoglycemic potential of almond hull extract in vitro and in vivo. The in vitro results showed that, among the 0 %, 30 %, 50 % and 80 % ethanol extracts of almond hull, the 50 % ethanol extracts demonstrated the most significant inhibition of digestive enzymes. With in vivo tests, the blood glucose concentration in the control rats increased sharply near the peak glucose level in 15 min, while the peak glucose level of rats loading the almond hull extract occurred in 30 min. Therefore, almond hull extract can reduce postprandial blood glucose levels and delay the time to peak by about 15 min, providing a sustained and gradual release of energy.
{"title":"Inhibition and effect of almond hull extract on activities of α-amylase and α-glucosidase, and postprandial glucose in normal SD rats","authors":"Yuyang Yao , Juer Liu , Qiming Miao , Xinyue Zhu , Lu Sun , Wei Hua , Na Zhang , Guangwei Huang , Roger Ruan , Yanling Cheng , Shengquan Mi","doi":"10.1016/j.jff.2024.106624","DOIUrl":"10.1016/j.jff.2024.106624","url":null,"abstract":"<div><div>Controlling postprandial hyperglycemia is considered as a viable prophylactic treatment for type 2 diabetes mellitus. A major strategy in decreasing postprandial glucose involves inhibition of the carbohydrate-hydrolyzing enzymes, α-amylase and α-glucosidase. The aim of this study was to evaluate the hypoglycemic potential of almond hull extract in vitro and in vivo. The in vitro results showed that, among the 0 %, 30 %, 50 % and 80 % ethanol extracts of almond hull, the 50 % ethanol extracts demonstrated the most significant inhibition of digestive enzymes. With in vivo tests, the blood glucose concentration in the control rats increased sharply near the peak glucose level in 15 min, while the peak glucose level of rats loading the almond hull extract occurred in 30 min. Therefore, almond hull extract can reduce postprandial blood glucose levels and delay the time to peak by about 15 min, providing a sustained and gradual release of energy.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"123 ","pages":"Article 106624"},"PeriodicalIF":3.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143166691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-01DOI: 10.1016/j.jff.2024.106621
Bowen Fan , Hongbo Teng , Zerbo Patrice , Xv Wang , Mengqi Shi , Yaru Zhao , Wenxin Zhang , Yan Zhao , Zhongmei He , Ying Zong , Weijia Chen , Rui Du
Mammary glands Hyperplasia (MGH) is a benign condition common in middle-aged women. This study addresses the therapeutic effects of various deer antler medicinal materials on MGH, a topic of increasing interest. The research compares the anti-MGH effects of these materials and investigates their mechanisms. Results indicate that deer antler materials inhibited abnormal proliferation in MCF-10 A cells and improved conditions in MGH rats particularly, Cervus nippon antler (CNA) showed the most significant effects. CNA treatment reduced nipple diameter, improved organ index, and alleviated pathological hyperplasia. It also decreased lobules and alveoli in mammary glands, reduced ductal dilation, and regulated sex hormone levels and receptor expression in MGH rats' serum. Transcriptomic analyses revealed that CNA lowers expression of RANKL, NF-κB, and Cyclin D1 in mammary tissue, inhibiting progress through the G1/S phase of mammary cells and inhibiting proliferation. These findings suggest that CNA could be an effective treatment for MGH, warranting further exploration of its mechanisms and applications in breast disease.
{"title":"Cervus nippon antler inhibits hormone disorder induced mammary gland hyperplasia by regulating the cell cycle","authors":"Bowen Fan , Hongbo Teng , Zerbo Patrice , Xv Wang , Mengqi Shi , Yaru Zhao , Wenxin Zhang , Yan Zhao , Zhongmei He , Ying Zong , Weijia Chen , Rui Du","doi":"10.1016/j.jff.2024.106621","DOIUrl":"10.1016/j.jff.2024.106621","url":null,"abstract":"<div><div>Mammary glands Hyperplasia (MGH) is a benign condition common in middle-aged women. This study addresses the therapeutic effects of various deer antler medicinal materials on MGH, a topic of increasing interest. The research compares the anti-MGH effects of these materials and investigates their mechanisms. Results indicate that deer antler materials inhibited abnormal proliferation in MCF-10 A cells and improved conditions in MGH rats particularly, <em>Cervus nippon</em> antler (CNA) showed the most significant effects. CNA treatment reduced nipple diameter, improved organ index, and alleviated pathological hyperplasia. It also decreased lobules and alveoli in mammary glands, reduced ductal dilation, and regulated sex hormone levels and receptor expression in MGH rats' serum. Transcriptomic analyses revealed that CNA lowers expression of RANKL, NF-κB, and Cyclin D1 in mammary tissue, inhibiting progress through the G1/S phase of mammary cells and inhibiting proliferation. These findings suggest that CNA could be an effective treatment for MGH, warranting further exploration of its mechanisms and applications in breast disease.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"123 ","pages":"Article 106621"},"PeriodicalIF":3.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143166692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-01DOI: 10.1016/j.jff.2024.106615
Cong Yin , Rui Fang , Yue Xu , Kui Li , Tingyang Ai , Jiawei Wan , Yonghua Qin , Xiaoguang Lyu , Hong Liu , Rui Qin , Lindong Yang , Hairong Xiong , Jiao Liu
Safflower is a widely utilized food and medicinal herb. The safflower petal is often used for tea-making or food colorant since the water-extract contains a lot of biologically active ingredients. This study aimed to evaluate the impacts of safflower petal water-extract (SE) on blood glucose regulation. The results demonstrated that both long-term and acute consumption of SE ameliorated the blood glucose regulation successfully. Mechanistically, SE suppressed the hepatic gluconeogenesis, which was involved with the protein kinase B (AKT)/forkhead transcription factor 1 (FoxO1) signaling pathway. In addition, SE modulated the gut microbiota that is related to host glucose regulation. Finally, drinking safflower petal-tea could efficiently decrease the healthy participants' blood glucose at two hours after breakfast. Hence, this study proved that drinking safflower petal-tea can be a feasible way to maintain stable blood glucose, and SE can be used as a potential function food ingredient for the prevention and amelioration of hyperglycemia.
{"title":"Safflower petal water-extract consumption reduces blood glucose via modulating hepatic gluconeogenesis and gut microbiota","authors":"Cong Yin , Rui Fang , Yue Xu , Kui Li , Tingyang Ai , Jiawei Wan , Yonghua Qin , Xiaoguang Lyu , Hong Liu , Rui Qin , Lindong Yang , Hairong Xiong , Jiao Liu","doi":"10.1016/j.jff.2024.106615","DOIUrl":"10.1016/j.jff.2024.106615","url":null,"abstract":"<div><div>Safflower is a widely utilized food and medicinal herb. The safflower petal is often used for tea-making or food colorant since the water-extract contains a lot of biologically active ingredients. This study aimed to evaluate the impacts of safflower petal water-extract (SE) on blood glucose regulation. The results demonstrated that both long-term and acute consumption of SE ameliorated the blood glucose regulation successfully. Mechanistically, SE suppressed the hepatic gluconeogenesis, which was involved with the protein kinase B (AKT)/forkhead transcription factor 1 (FoxO1) signaling pathway. In addition, SE modulated the gut microbiota that is related to host glucose regulation. Finally, drinking safflower petal-tea could efficiently decrease the healthy participants' blood glucose at two hours after breakfast. Hence, this study proved that drinking safflower petal-tea can be a feasible way to maintain stable blood glucose, and SE can be used as a potential function food ingredient for the prevention and amelioration of hyperglycemia.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"123 ","pages":"Article 106615"},"PeriodicalIF":3.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142746051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-01DOI: 10.1016/j.jff.2024.106591
Ling-Yu Zhang , Liuyan Ma , Lian-zhong Luo , Jian Li
Scope: Pickled radish is a traditional fermented food known for its health benefits, however, evidence supporting its role in regulating gastrointestinal function is limited. This study aimed to investigate the beneficial effect of pickled radish on DSS-induced colitis in mice. Method and Results: Divide the mice into four groups: control, DSS model, low-dose (DSS+ 5 % pickled radish powder), and high-dose (DSS+ 10 % pickled radish powder). Colitis was induced by 3 % DSS administration in drinking water for eight days. Body weight loss, disease activity index (DAI), colon length, histology, and levels of inflammatory markers were measured to evaluate the effects of pickled radish on colitis. The results indicate that pickled radish significantly eased symptoms of colitis in mice induced by DSS, dose-dependently reducing DAI, reversed colon shortening, alleviating pathological damage to colon tissue and inflammatory response. Mechanistic investigation revealed that pickled radish upregulated the expression of tight junction-related genes (Zo-1, E-cadherin) and down-regulated inflammation-related genes (Il-1β, Il-6, TNF-α). Moreover, low-dose pickled radish significantly reversed the imbalance of gut microbiota and increased SCFAs. Conclusion: These findings suggest the potential of pickled radish in functional foods targeting the improvement of gastrointestinal health.
{"title":"Pickled radish alleviates the progression of colitis in mice by reducing inflammation and repairing intestinal barrier","authors":"Ling-Yu Zhang , Liuyan Ma , Lian-zhong Luo , Jian Li","doi":"10.1016/j.jff.2024.106591","DOIUrl":"10.1016/j.jff.2024.106591","url":null,"abstract":"<div><div>Scope: Pickled radish is a traditional fermented food known for its health benefits, however, evidence supporting its role in regulating gastrointestinal function is limited. This study aimed to investigate the beneficial effect of pickled radish on DSS-induced colitis in mice. Method and Results: Divide the mice into four groups: control, DSS model, low-dose (DSS+ 5 % pickled radish powder), and high-dose (DSS+ 10 % pickled radish powder). Colitis was induced by 3 % DSS administration in drinking water for eight days. Body weight loss, disease activity index (DAI), colon length, histology, and levels of inflammatory markers were measured to evaluate the effects of pickled radish on colitis. The results indicate that pickled radish significantly eased symptoms of colitis in mice induced by DSS, dose-dependently reducing DAI, reversed colon shortening, alleviating pathological damage to colon tissue and inflammatory response. Mechanistic investigation revealed that pickled radish upregulated the expression of tight junction-related genes (Zo-1, E-cadherin) and down-regulated inflammation-related genes (Il-1β, Il-6, TNF-α). Moreover, low-dose pickled radish significantly reversed the imbalance of gut microbiota and increased SCFAs. Conclusion: These findings suggest the potential of pickled radish in functional foods targeting the improvement of gastrointestinal health.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"123 ","pages":"Article 106591"},"PeriodicalIF":3.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143166680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-01DOI: 10.1016/j.jff.2024.106602
Diah Kartika Pratami , Abdul Mun'im , Muhamad Sahlan , Shigenori Kumazawa , Ibrahim Jantan , Siti Irma Rahmawati , Masteria Yunovilsa Putra , Asep Bayu
Propolis, a functional food ingredient, has diverse constituents that give it pharmacological properties. This systematic review aimed to provide a summary of propolis metabolomics studies throughout the past decade. Covidence systematic review platform was used to summarize propolis metabolomics papers from 2014 to 2024 in Scopus, PubMed, and Web of Science. Thirty-three articles were conducted in vitro and in vivo, providing information on metabolite profiling (molecular markers and metabolic pathway information) and its phytogeographical discrimination (botanical origin and regional discrimination). Advanced data analysis, multivariate statistical analysis, bioinformatics tools, and machine learning were used in addition to chromatographic, spectroscopic, and spectrometric procedures. Propolis composition can be affected by plant sources, bee species, and location. This review examined the limitations of propolis metabolomic studies and recommended ways to improve their quality and applicability. It provides information on propolis in the metabolic pathways of various diseases and its metabolites as functional food ingredients.
{"title":"A systematic review of metabolomics studies on metabolite profiling and phytogeographical discrimination of propolis","authors":"Diah Kartika Pratami , Abdul Mun'im , Muhamad Sahlan , Shigenori Kumazawa , Ibrahim Jantan , Siti Irma Rahmawati , Masteria Yunovilsa Putra , Asep Bayu","doi":"10.1016/j.jff.2024.106602","DOIUrl":"10.1016/j.jff.2024.106602","url":null,"abstract":"<div><div>Propolis, a functional food ingredient, has diverse constituents that give it pharmacological properties. This systematic review aimed to provide a summary of propolis metabolomics studies throughout the past decade. Covidence systematic review platform was used to summarize propolis metabolomics papers from 2014 to 2024 in Scopus, PubMed, and Web of Science. Thirty-three articles were conducted <em>in vitro</em> and <em>in vivo</em>, providing information on metabolite profiling (molecular markers and metabolic pathway information) and its phytogeographical discrimination (botanical origin and regional discrimination). Advanced data analysis, multivariate statistical analysis, bioinformatics tools, and machine learning were used in addition to chromatographic, spectroscopic, and spectrometric procedures. Propolis composition can be affected by plant sources, bee species, and location. This review examined the limitations of propolis metabolomic studies and recommended ways to improve their quality and applicability. It provides information on propolis in the metabolic pathways of various diseases and its metabolites as functional food ingredients.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"123 ","pages":"Article 106602"},"PeriodicalIF":3.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143166676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aging-induced gut dysbiosis may contribute to inflammation and obesity, potentially elevating the risk of age-related chronic diseases. In this study, we aimed to investigate the effects of diet supplemented with 8% eggshell membrane (ESM) on gut microbial composition in chronologically aged C57BL/6J male mice (53-week-old) compared to those of young (8-week-old) and age-matched control mice. After a 24-week dietary intervention, the ESM-fed group exhibited an increased abundance of butyrate-producing and valerate-producing bacteria, accompanied by a significant increase in the cecal concentrations of isobutyrate, isovalerate, and valerate. Furthermore, the ESM-supplemented diet led to reduced expression of Srebf1, Acaca, Elovl6, and Scd1, suggesting a potential role for short-chain fatty acids in the suppression of hepatic lipogenesis. Additionally, reduced hepatic triglyceride levels showed an association with the downregulation of Atgl and Cpt1a, two key lipolysis-associated genes. These findings highlight the potential of ESM as a functional food ingredient that can support healthy aging.
{"title":"Eggshell membrane, a resistant protein, possesses prebiotic-like properties in improving gut microbiota composition and gut-liver axes in chronologically aged C57BL/6J male mice","authors":"Dwina Juliana Warman , Huijuan Jia , Yukio Hasebe , Hisanori Kato","doi":"10.1016/j.jff.2024.106610","DOIUrl":"10.1016/j.jff.2024.106610","url":null,"abstract":"<div><div>Aging-induced gut dysbiosis may contribute to inflammation and obesity, potentially elevating the risk of age-related chronic diseases. In this study, we aimed to investigate the effects of diet supplemented with 8% eggshell membrane (ESM) on gut microbial composition in chronologically aged C57BL/6J male mice (53-week-old) compared to those of young (8-week-old) and age-matched control mice. After a 24-week dietary intervention, the ESM-fed group exhibited an increased abundance of butyrate-producing and valerate-producing bacteria, accompanied by a significant increase in the cecal concentrations of isobutyrate, isovalerate, and valerate. Furthermore, the ESM-supplemented diet led to reduced expression of <em>Srebf1</em>, <em>Acaca</em>, <em>Elovl6</em>, and <em>Scd1</em>, suggesting a potential role for short-chain fatty acids in the suppression of hepatic lipogenesis. Additionally, reduced hepatic triglyceride levels showed an association with the downregulation of <em>Atgl</em> and <em>Cpt1a</em>, two key lipolysis-associated genes. These findings highlight the potential of ESM as a functional food ingredient that can support healthy aging.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"123 ","pages":"Article 106610"},"PeriodicalIF":3.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143166690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-01DOI: 10.1016/j.jff.2024.106604
Yongmei Qi , Yao Dong , Jiayue Gu , Yihui Cai , Yanyan Zhang , Zhonghui Gai , Mei Han
In vitro tests assessed heat tolerance, bile salt hydrolysis, intestinal adhesion, gastrointestinal environmental tolerance, hemolysis, gelatin hydrolysis, antibiotic sensitivity, inhibition of pathogenic bacteria, and toxicity of Lactobacillus acidophilus LA85. The safety of LA85 was verified by acute oral toxicity test. Genome analysis revealed no virulence, pathogenicity, or biogenic amine synthesis genes in LA85, but one drug resistance gene. LA85 displayed strong heat tolerance, intestinal adhesion, and gastrointestinal environment tolerance, with bile salt and gelatin hydrolysis abilities, and no hemolysis. It was sensitive to European Food Safety Authority proposed antibiotics, had inhibitory effects on common pathogenic bacteria, and both its fermentation broth and cells were non-toxic. Acute oral toxicity test confirmed that LA85 (2 × 1010 CFU/kg) was safe and non-toxic in mice. The safety evaluation presented in this paper firmly establishes LA85 as a safe and effective probiotic candidate for use in a wide range of food and medical applications.
{"title":"In-depth genomic identification and safety evaluation of Lactobacillus Acidophilus LA85","authors":"Yongmei Qi , Yao Dong , Jiayue Gu , Yihui Cai , Yanyan Zhang , Zhonghui Gai , Mei Han","doi":"10.1016/j.jff.2024.106604","DOIUrl":"10.1016/j.jff.2024.106604","url":null,"abstract":"<div><div>In vitro tests assessed heat tolerance, bile salt hydrolysis, intestinal adhesion, gastrointestinal environmental tolerance, hemolysis, gelatin hydrolysis, antibiotic sensitivity, inhibition of pathogenic bacteria, and toxicity of <em>Lactobacillus acidophilus</em> LA85. The safety of LA85 was verified by acute oral toxicity test. Genome analysis revealed no virulence, pathogenicity, or biogenic amine synthesis genes in LA85, but one drug resistance gene. LA85 displayed strong heat tolerance, intestinal adhesion, and gastrointestinal environment tolerance, with bile salt and gelatin hydrolysis abilities, and no hemolysis. It was sensitive to European Food Safety Authority proposed antibiotics, had inhibitory effects on common pathogenic bacteria, and both its fermentation broth and cells were non-toxic. Acute oral toxicity test confirmed that LA85 (2 × 10<sup>10</sup> CFU/kg) was safe and non-toxic in mice. The safety evaluation presented in this paper firmly establishes LA85 as a safe and effective probiotic candidate for use in a wide range of food and medical applications.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"123 ","pages":"Article 106604"},"PeriodicalIF":3.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142745876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-01DOI: 10.1016/j.jff.2024.106606
Shilei Wang , Jianchang Jin , Yi Wu , Nuo Chen , Baiyi Chu , Wei Wang , Nan Wang
Activation of the Antioxidant response element (ARE) pathway contributes to protecting cells against oxidative stress. In the previous study, Asn-Cys-Ala and Cys-Thr-Ala derived from soft-shelled turtles demonstrated potent ARE-luciferase inducer activity in the HepG2 cell model. In this study, the effects of Asn-Cys-Ala and Cys-Thr-Ala on the Nuclear factor (erythroid-derived 2)-like 2/ Kelch-like ECH-associated protein 1 (Nrf2/Keap1) system in HepG2 cells were studied. The results showed that Asn-Cys-Ala and Cys-Thr-Ala regulated the Nrf2/ARE pathway by stabilizing the Nrf2 levels, which was achieved by decreasing the levels of Keap1. The EC50 values for the cellular antioxidant activity of Asn-Cys-Ala and Cys-Thr-Ala were 81.53 and 58.60 μM, respectively. And the results also demonstrated that treatment with Asn-Cys-Ala and Cys-Thr-Ala could reduce the H2O2-induced changes in intracellular Reactive Oxygen Species (ROS), Malondialdehyde (MDA) levels, and antioxidant enzyme activity (Superoxide dismutase, SOD; Glutathione peroxidase, GSH-Px; Catalase, CAT) (p < 0.05).
{"title":"Two turtle-derived tripeptides as ARE-luciferase inducers suppress H2O2-induced oxidative stress","authors":"Shilei Wang , Jianchang Jin , Yi Wu , Nuo Chen , Baiyi Chu , Wei Wang , Nan Wang","doi":"10.1016/j.jff.2024.106606","DOIUrl":"10.1016/j.jff.2024.106606","url":null,"abstract":"<div><div>Activation of the Antioxidant response element (ARE) pathway contributes to protecting cells against oxidative stress. In the previous study, Asn-Cys-Ala and Cys-Thr-Ala derived from soft-shelled turtles demonstrated potent ARE-luciferase inducer activity in the HepG2 cell model. In this study, the effects of Asn-Cys-Ala and Cys-Thr-Ala on the Nuclear factor (erythroid-derived 2)-like 2/ Kelch-like ECH-associated protein 1 (Nrf2/Keap1) system in HepG2 cells were studied. The results showed that Asn-Cys-Ala and Cys-Thr-Ala regulated the Nrf2/ARE pathway by stabilizing the Nrf2 levels, which was achieved by decreasing the levels of Keap1. The EC<sub>50</sub> values for the cellular antioxidant activity of Asn-Cys-Ala and Cys-Thr-Ala were 81.53 and 58.60 μM, respectively. And the results also demonstrated that treatment with Asn-Cys-Ala and Cys-Thr-Ala could reduce the H<sub>2</sub>O<sub>2</sub>-induced changes in intracellular Reactive Oxygen Species (ROS), Malondialdehyde (MDA) levels, and antioxidant enzyme activity (Superoxide dismutase, SOD; Glutathione peroxidase, GSH-Px; Catalase, CAT) (<em>p</em> < 0.05).</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"123 ","pages":"Article 106606"},"PeriodicalIF":3.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143166688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-01DOI: 10.1016/j.jff.2024.106618
Eman E. Yassien , Maha M. Bikheet , Asmaa H. Zaki , Moshira M. Shaban , Areej A. Almuraee , Awatif M. Almehmadi , Hanaa S.S. Gazwi , Waleed M. Abdel-Aleem
Soybean and bee drone milk are promising functional food candidates due to their bioactive compounds and health benefits. This study evaluated fortified soymilk with drone milk (BSDM) in mitigating cisplatin-induced testicular toxicity and explored the mechanisms involved. Different BSDM concentrations were analyzed for physicochemical properties, antioxidant capacity, and sensory attributes. Male Wistar rats were divided into control, BSDM, cisplatin, and cisplatin + BSDM groups. Sperm motility, hormone levels, oxidative stress markers, apoptosis, and inflammation were assessed. BSDM supplementation enhanced phenolic levels, antioxidant potential, nutritional characteristics, and consumer acceptance of soymilk. Rats treated with cisplatin showed reduced testes weight, sperm parameters, hormone levels, and testicular glutathione, along with increased malondialdehyde, total nitrite, NF-κB, caspase-3,and TNF-α expression. BSDM administration mitigated testicular toxicity, restored function, and repaired histological abnormalities by regulating oxidative stress, apoptosis, and inflammation. These findings suggest BSDM as a functional food for alleviating cisplatin-induced testicular toxicity.
{"title":"Therapeutic effects of bee drone milk-fortified soymilk on cisplatin-induced testicular toxicity: Mechanisms and potential benefits","authors":"Eman E. Yassien , Maha M. Bikheet , Asmaa H. Zaki , Moshira M. Shaban , Areej A. Almuraee , Awatif M. Almehmadi , Hanaa S.S. Gazwi , Waleed M. Abdel-Aleem","doi":"10.1016/j.jff.2024.106618","DOIUrl":"10.1016/j.jff.2024.106618","url":null,"abstract":"<div><div>Soybean and bee drone milk are promising functional food candidates due to their bioactive compounds and health benefits. This study evaluated fortified soymilk with drone milk (BSDM) in mitigating cisplatin-induced testicular toxicity and explored the mechanisms involved. Different BSDM concentrations were analyzed for physicochemical properties, antioxidant capacity, and sensory attributes. Male Wistar rats were divided into control, BSDM, cisplatin, and cisplatin + BSDM groups. Sperm motility, hormone levels, oxidative stress markers, apoptosis, and inflammation were assessed. BSDM supplementation enhanced phenolic levels, antioxidant potential, nutritional characteristics, and consumer acceptance of soymilk. Rats treated with cisplatin showed reduced testes weight, sperm parameters, hormone levels, and testicular glutathione, along with increased malondialdehyde, total nitrite, NF-κB, caspase-3,and TNF-α expression. BSDM administration mitigated testicular toxicity, restored function, and repaired histological abnormalities by regulating oxidative stress, apoptosis, and inflammation. These findings suggest BSDM as a functional food for alleviating cisplatin-induced testicular toxicity.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"123 ","pages":"Article 106618"},"PeriodicalIF":3.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143166695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-01DOI: 10.1016/j.jff.2024.106577
Kwang-Rim Baek , Hyun-Ji Lee , Kang-Hyeon Jeong , Bo-Ram Park , Soo-Jung Kim , Seung-Oh Seo
Yeast, particularly Saccharomyces cerevisiae, is widely regarded as a nutritional supplement in the food and feed industries. Notably, Saccharomyces boulardii is promoted as a probiotic strain for its efficacy in gastrointestinal disorders. Despite genetic similarities to S. cerevisiae, S. boulardii is distinguished from S. cerevisiae by unique features like cell wall composition, which is crucial for immune activation. This study evaluated the cell wall polysaccharide contents of S. boulardii and S. cerevisiae and their immunomodulatory activity on Raw 264.7 cell. Results showed an increased secretion of pro-inflammatory factors (TNF-α, IL-6, and NO) and the anti-inflammatory factor IL-10. Remarkably S. boulardii cell extracts had a more pronounced effect than S. cerevisiae cell extracts. The immunomodulatory activity is linked to the MAPK(ERK)/NF-κB pathway, activated by β-glucan and mannan via Dectin-1 and Dectin-2 receptors. This difference in immune response are attributed to variations in content of mannan and β-glucan in their cell walls.
{"title":"Parabiotic immunomodulatory activity of yeast cell wall polysaccharides from Saccharomyces cerevisiae and S. boulardii","authors":"Kwang-Rim Baek , Hyun-Ji Lee , Kang-Hyeon Jeong , Bo-Ram Park , Soo-Jung Kim , Seung-Oh Seo","doi":"10.1016/j.jff.2024.106577","DOIUrl":"10.1016/j.jff.2024.106577","url":null,"abstract":"<div><div>Yeast, particularly <em>Saccharomyces cerevisiae</em>, is widely regarded as a nutritional supplement in the food and feed industries. Notably, <em>Saccharomyces boulardii</em> is promoted as a probiotic strain for its efficacy in gastrointestinal disorders. Despite genetic similarities to <em>S. cerevisiae</em>, <em>S. boulardii</em> is distinguished from <em>S. cerevisiae</em> by unique features like cell wall composition, which is crucial for immune activation. This study evaluated the cell wall polysaccharide contents of <em>S. boulardii</em> and <em>S. cerevisiae</em> and their immunomodulatory activity on Raw 264.7 cell. Results showed an increased secretion of pro-inflammatory factors (TNF-α, IL-6, and NO) and the anti-inflammatory factor IL-10. Remarkably <em>S. boulardii</em> cell extracts had a more pronounced effect than <em>S. cerevisiae</em> cell extracts. The immunomodulatory activity is linked to the MAPK(ERK)/NF-κB pathway, activated by β-glucan and mannan via Dectin-1 and Dectin-2 receptors. This difference in immune response are attributed to variations in content of mannan and β-glucan in their cell walls.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"123 ","pages":"Article 106577"},"PeriodicalIF":3.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142745878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
扫码关注我们
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号