Pub Date : 2025-10-15DOI: 10.1016/j.jff.2025.107058
Kamila Goderska, Peace Oluwamuyiwa Obey
This analysis delves into the combined advantages of synbiotics - a mix of probiotics and prebiotics, in supporting gut health and overall wellness. Synbiotics have displayed potential in enhancing function reducing inflammation and promoting digestion. Research has indicated their effectiveness in managing conditions like rheumatoid arthritis decline in adults, non-alcoholic fatty liver disease (NAFLD) and type 2 diabetes. Despite their advantages, synbiotics encounter obstacles related to stability and effectiveness, particularly regarding the compatibility of components and the viability of strains. Lactobionic acid (LBA) an acid derived from lactose emerges as an addition to synbiotic formulations due to its antioxidant properties as well as its ability to stabilize and preserve. This examination underscores the benefits of incorporating LBA to enhance the stability and effectiveness of synbiotics urging exploration, through clinical trials to optimize formulation and assess their health impacts. This article is a review of the latest literature in the field of knowledge about synbiotics and lactobionic acid, which can provide a source of growth for probiotic microorganisms. The data presented and discussed in this narrative review were obtained from PubMed, ScienceDirect Scopus (Elsevier) and Web of Science (restricted to the years , 2002 to 2024 with the key words: probiotic, prebiotic and lactobionic acid).
该分析深入研究了益生菌和益生元在支持肠道健康和整体健康方面的综合优势。合成制剂在增强功能、减少炎症和促进消化方面显示出潜力。研究表明，它们对治疗成人类风湿性关节炎、非酒精性脂肪性肝病（NAFLD）和2型糖尿病等疾病有效。尽管具有优势，但合生剂在稳定性和有效性方面遇到了障碍，特别是在组分的相容性和菌株的生存能力方面。乳酸菌酸（LBA）是一种从乳糖中提取的酸，由于其抗氧化特性以及稳定和保存的能力而被添加到合成配方中。本研究强调了加入LBA对增强合生素的稳定性和有效性的益处，敦促探索，通过临床试验来优化配方并评估其对健康的影响。本文综述了合成菌和乳酸菌的最新研究进展，为益生菌微生物的生长提供了新的来源。这篇叙述性综述所呈现和讨论的数据来自PubMed、ScienceDirect Scopus （Elsevier）和Web of Science（限于2002年至2024年，关键词：益生菌、益生元和乳酸）。
{"title":"Characteristics of synbiotics and lactobionic acid","authors":"Kamila Goderska, Peace Oluwamuyiwa Obey","doi":"10.1016/j.jff.2025.107058","DOIUrl":"10.1016/j.jff.2025.107058","url":null,"abstract":"<div><div>This analysis delves into the combined advantages of synbiotics - a mix of probiotics and prebiotics, in supporting gut health and overall wellness. Synbiotics have displayed potential in enhancing function reducing inflammation and promoting digestion. Research has indicated their effectiveness in managing conditions like rheumatoid arthritis decline in adults, non-alcoholic fatty liver disease (NAFLD) and type 2 diabetes. Despite their advantages, synbiotics encounter obstacles related to stability and effectiveness, particularly regarding the compatibility of components and the viability of strains. Lactobionic acid (LBA) an acid derived from lactose emerges as an addition to synbiotic formulations due to its antioxidant properties as well as its ability to stabilize and preserve. This examination underscores the benefits of incorporating LBA to enhance the stability and effectiveness of synbiotics urging exploration, through clinical trials to optimize formulation and assess their health impacts. This article is a review of the latest literature in the field of knowledge about synbiotics and lactobionic acid, which can provide a source of growth for probiotic microorganisms. The data presented and discussed in this narrative review were obtained from PubMed, ScienceDirect Scopus (Elsevier) and Web of Science (restricted to the years , 2002 to 2024 with the key words: probiotic, prebiotic and lactobionic acid).</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"134 ","pages":"Article 107058"},"PeriodicalIF":4.0,"publicationDate":"2025-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145325382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-10-14DOI: 10.1016/j.jff.2025.107051
Xiaoyue Zhang , Yuanyuan Sun , Zixuan Zhang , Xuchun Zhu , Shan He , Linyi Zhou , Mingzheng Huang , Hongzhi Liu
Rosa roxburghii Tratt pomace, a by-product of processing Rosa roxburghii Tratt, constitutes about 45 % of the fresh fruit mass and contains a high proportion of nutritive active ingredients. Therefore, it has important applications in food, cosmetics and medical products. Extensive research has been conducted on the main active components of Rosa roxburghii Tratt pomace, including their content and composition. This paper presents a systematic review of the structure and extraction methods of the active constituents (polysaccharides, dietary fiber, polyphenols and flavonoids, Vitamin C, superoxide dismutase, and other bioactive compounds) in the Rosa roxburghii Tratt pomace. It further summarizes their functional properties and the progress made in applications. This review concludes that these studies can provide references for the future development and utilization of the Rosa roxburghii Tratt pomace, so as to extend the industrial chain, improve the efficiency of resource utilization, reduce environmental pollution, and achieve economic and ecological benefits.
{"title":"A review: Extraction of active ingredients, functional properties of Rosa roxburghii Tratt pomace and its application","authors":"Xiaoyue Zhang , Yuanyuan Sun , Zixuan Zhang , Xuchun Zhu , Shan He , Linyi Zhou , Mingzheng Huang , Hongzhi Liu","doi":"10.1016/j.jff.2025.107051","DOIUrl":"10.1016/j.jff.2025.107051","url":null,"abstract":"<div><div><em>Rosa roxburghii</em> Tratt pomace, a by-product of processing <em>Rosa roxburghii</em> Tratt, constitutes about 45 % of the fresh fruit mass and contains a high proportion of nutritive active ingredients. Therefore, it has important applications in food, cosmetics and medical products. Extensive research has been conducted on the main active components of <em>Rosa roxburghii</em> Tratt pomace, including their content and composition. This paper presents a systematic review of the structure and extraction methods of the active constituents (polysaccharides, dietary fiber, polyphenols and flavonoids, Vitamin C, superoxide dismutase, and other bioactive compounds) in the <em>Rosa roxburghii</em> Tratt pomace. It further summarizes their functional properties and the progress made in applications. This review concludes that these studies can provide references for the future development and utilization of the <em>Rosa roxburghii</em> Tratt pomace, so as to extend the industrial chain, improve the efficiency of resource utilization, reduce environmental pollution, and achieve economic and ecological benefits.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"134 ","pages":"Article 107051"},"PeriodicalIF":4.0,"publicationDate":"2025-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145325380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-10-13DOI: 10.1016/j.jff.2025.107063
Junya Takegaki , Takumi Yokokawa , Risako Mori , Daiki Nakano , Aya Matsushita , Seiko Koizumi , Satoshi Fujita
Collagen peptides (CPTs) increase muscle mass with resistance exercise training. However, little is known regarding the effect of CPT administration on anabolic signals in skeletal muscle. In this study, we prepared two types of CPTs, one rich in hydroxyprolyl-glycine (CPT-A) and the other rich in hydroxyprolyl-glycine, prolyl-hydroxyproline, and alanyl-hydroxyprolyl-glycine (CPT–B) and investigated the effects of these peptides on skeletal muscle mTORC1 in mice. Initially, the effects of individual administration of each CPT were investigated. CPT-B increased the expression of phosphorylated rpS6 at 180 min post-administration which was not observed after the administration of whey protein. CPT-A did not induce any significant changes in mTORC1 signaling. Subsequently, their combined effects with whey protein were evaluated. However, the delayed activation was not observed under these conditions. These findings suggest that the CPT-B alone has the potency to activate mTORC1 progressively over time, with a slower onset than whey protein.
{"title":"Effect of collagen peptides rich in hydroxyprolyl-glycine on anabolic signals in mouse skeletal muscle","authors":"Junya Takegaki , Takumi Yokokawa , Risako Mori , Daiki Nakano , Aya Matsushita , Seiko Koizumi , Satoshi Fujita","doi":"10.1016/j.jff.2025.107063","DOIUrl":"10.1016/j.jff.2025.107063","url":null,"abstract":"<div><div>Collagen peptides (CPTs) increase muscle mass with resistance exercise training. However, little is known regarding the effect of CPT administration on anabolic signals in skeletal muscle. In this study, we prepared two types of CPTs, one rich in hydroxyprolyl-glycine (CPT-A) and the other rich in hydroxyprolyl-glycine, prolyl-hydroxyproline, and alanyl-hydroxyprolyl-glycine (CPT–B) and investigated the effects of these peptides on skeletal muscle mTORC1 in mice. Initially, the effects of individual administration of each CPT were investigated. CPT-B increased the expression of phosphorylated rpS6 at 180 min post-administration which was not observed after the administration of whey protein. CPT-A did not induce any significant changes in mTORC1 signaling. Subsequently, their combined effects with whey protein were evaluated. However, the delayed activation was not observed under these conditions. These findings suggest that the CPT-B alone has the potency to activate mTORC1 progressively over time, with a slower onset than whey protein.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"134 ","pages":"Article 107063"},"PeriodicalIF":4.0,"publicationDate":"2025-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145325609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-10-13DOI: 10.1016/j.jff.2025.107066
Danyi Liu , Run Quan , Xiaolong Wu , Zhengchen Chong , JunJie Zhang , Rongxu Liu , Jianchun Han , Yu Yang
In this study, porcine small intestinal glycoproteins (PSIG-1) was extracted and purified from the heparin-processing wastewater of porcine small intestines; its structure was characterized, and its antioxidant activity was evaluated. The optimized extraction conditions were 0.3 mol/L NaOH, liquid-solid ratio of 1:20, and extraction at 60 °C for 3.5 h. The extraction yield reached 11.46 ± 1.73 %. PSIG-1 consists of 83.52 ± 0.39 % proteins and 12.42 ± 0.41 % polysaccharides. PSIG-1 was primarily composed of a 70 kDa glycoprotein. Structural analysis revealed that PSIG-1 possesses the O-glycopeptide linkage and the pyranose cyclic sugar chain conformation that protects its antioxidant activity, with its secondary structure predominantly comprising random coils. In cellular experiments, PSIG-1 significantly enhanced the activities of glutathione peroxidase, superoxide dismutase, and catalase while reducing intracellular malondialdehyde levels in RAW264.7 cells subjected to H2O2-induced oxidative damage, demonstrating strong antioxidant activity. This study reveals that PSIG-1 has potential applications as an antioxidant in functional foods.
{"title":"Extraction, structural characterization and in vitro antioxidant activity of porcine small intestinal glycoproteins from heparin processing wastewater","authors":"Danyi Liu , Run Quan , Xiaolong Wu , Zhengchen Chong , JunJie Zhang , Rongxu Liu , Jianchun Han , Yu Yang","doi":"10.1016/j.jff.2025.107066","DOIUrl":"10.1016/j.jff.2025.107066","url":null,"abstract":"<div><div>In this study, porcine small intestinal glycoproteins (PSIG-1) was extracted and purified from the heparin-processing wastewater of porcine small intestines; its structure was characterized, and its antioxidant activity was evaluated. The optimized extraction conditions were 0.3 mol/L NaOH, liquid-solid ratio of 1:20, and extraction at 60 °C for 3.5 h. The extraction yield reached 11.46 ± 1.73 %. PSIG-1 consists of 83.52 ± 0.39 % proteins and 12.42 ± 0.41 % polysaccharides. PSIG-1 was primarily composed of a 70 kDa glycoprotein. Structural analysis revealed that PSIG-1 possesses the O-glycopeptide linkage and the pyranose cyclic sugar chain conformation that protects its antioxidant activity, with its secondary structure predominantly comprising random coils. In cellular experiments, PSIG-1 significantly enhanced the activities of glutathione peroxidase, superoxide dismutase, and catalase while reducing intracellular malondialdehyde levels in RAW264.7 cells subjected to H<sub>2</sub>O<sub>2</sub>-induced oxidative damage, demonstrating strong antioxidant activity. This study reveals that PSIG-1 has potential applications as an antioxidant in functional foods.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"134 ","pages":"Article 107066"},"PeriodicalIF":4.0,"publicationDate":"2025-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145325610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-10-12DOI: 10.1016/j.jff.2025.107061
Jiarong Kuang , Jie Luo , Xia He , Tao Yu , Yan Zhou , Xinfeng Zhang , Zhaoxia Liu , Tingtao Chen
Background: Polycystic Ovary Syndrome (PCOS), a prevalent endocrine disorder in women, is characterized by insulin resistance (IR), obesity, and gut dysbiosis, driving metabolic and reproductive dysfunction. Metformin, though first-line for PCOS, is often limited by gastrointestinal intolerance, reducing adherence. Thus, safe adjuncts are urgently needed to improve metabolic control and treatment tolerability. This trial evaluated whether Lactobacillus acidophilus (L. acidophilus) JYLA-126 could enhance metformin's efficacy and tolerability in women with PCOS.
Methods: In this 12-week randomized controlled trial, 100 women (18–40 years) with PCOS, IR, and obesity were randomized to receive metformin plus placebo or metformin plus L. acidophilus JYLA-126. Outcomes included anthropometrics, menstrual regularity, metabolic/hormonal profiles, gut microbiota, and adverse events. Analyses involved t-tests, ANOVA, and Pearson correlations.
Results: Both groups improved with metformin, but the probiotic group showed greater weight loss and metabolic benefits, including lower fasting glucose (4.97 ± 0.53 vs. 5.53 ± 0.84 mmol/L, p < 0.001) and HOMA-IR (3.35 ± 1.48 vs. 4.40 ± 2.29, p < 0.05) at 1 month, and more favorable hormonal changes (LH: 7.68 ± 1.15 vs. 8.92 ± 1.79 U/L; AMH: 6.40 ± 1.88 vs. 7.90 ± 2.39 ng/mL, p < 0.05) at 3 months. Favorable shifts in gut microbiota (increased Faecalibacterium and Ruminococcus, reduced Collinsella) were also observed. The combination reduced gastrointestinal side effects, and microbiota changes correlated with PCOS/IR biomarkers.
Conclusion: L. acidophilus JYLA-126 supplementation may provide additional metabolic, hormonal, and gastrointestinal benefits to metformin therapy in PCOS, supporting its potential as a useful nutritional adjunct.
背景：多囊卵巢综合征（PCOS）是一种常见的女性内分泌疾病，以胰岛素抵抗（IR）、肥胖和肠道生态失调为特征，可导致代谢和生殖功能障碍。二甲双胍虽然是治疗多囊卵巢综合征的一线药物，但常常受到胃肠不耐受的限制，降低了依从性。因此，迫切需要安全的辅助药物来改善代谢控制和治疗耐受性。本试验评估嗜酸乳杆菌（L. acidophilus） jila -126是否能提高二甲双胍对PCOS女性的疗效和耐受性。方法：在这项为期12周的随机对照试验中，100名患有PCOS， IR和肥胖的女性（18-40岁）随机接受二甲双胍加安慰剂或二甲双胍加嗜酸乳杆菌jila -126。结果包括人体测量、月经规律、代谢/激素谱、肠道微生物群和不良事件。分析包括t检验、方差分析和Pearson相关性。结果：两组使用二甲双胍均有改善，但益生菌组表现出更大的减肥和代谢益处，包括1个月时空腹血糖（4.97±0.53 vs. 5.53±0.84 mmol/L, p < 0.001）和HOMA-IR（3.35±1.48 vs. 4.40±2.29,p < 0.05）降低，3个月时更有利的激素变化（LH: 7.68±1.15 vs. 8.92±1.79 U/L; AMH: 6.40±1.88 vs. 7.90±2.39 ng/mL, p < 0.05）。肠道菌群的有利变化（粪杆菌和瘤胃球菌增加，大肠杆菌减少）也被观察到。联合用药减少了胃肠道副作用，微生物群变化与PCOS/IR生物标志物相关。结论：补充嗜酸乳杆菌jila -126可能为PCOS患者的二甲双胍治疗提供额外的代谢、激素和胃肠道益处，支持其作为有用的营养辅助剂的潜力。
{"title":"Probiotic Lactobacillus acidophilus JYLA-126 improves metabolic outcomes in polycystic ovary syndrome: a randomized controlled trial","authors":"Jiarong Kuang , Jie Luo , Xia He , Tao Yu , Yan Zhou , Xinfeng Zhang , Zhaoxia Liu , Tingtao Chen","doi":"10.1016/j.jff.2025.107061","DOIUrl":"10.1016/j.jff.2025.107061","url":null,"abstract":"<div><div><em>Background:</em> Polycystic Ovary Syndrome (PCOS), a prevalent endocrine disorder in women, is characterized by insulin resistance (IR), obesity, and gut dysbiosis, driving metabolic and reproductive dysfunction. Metformin, though first-line for PCOS, is often limited by gastrointestinal intolerance, reducing adherence. Thus, safe adjuncts are urgently needed to improve metabolic control and treatment tolerability. This trial evaluated whether <em>Lactobacillus acidophilus</em> (<em>L. acidophilus</em>) JYLA-126 could enhance metformin's efficacy and tolerability in women with PCOS.</div><div><em>Methods:</em> In this 12-week randomized controlled trial, 100 women (18–40 years) with PCOS, IR, and obesity were randomized to receive metformin plus placebo or metformin plus L. <em>acidophilus</em> JYLA-126. Outcomes included anthropometrics, menstrual regularity, metabolic/hormonal profiles, gut microbiota, and adverse events. Analyses involved <em>t</em>-tests, ANOVA, and Pearson correlations.</div><div><em>Results:</em> Both groups improved with metformin, but the probiotic group showed greater weight loss and metabolic benefits, including lower fasting glucose (4.97 ± 0.53 vs. 5.53 ± 0.84 mmol/L, <em>p</em> < 0.001) and HOMA-IR (3.35 ± 1.48 vs. 4.40 ± 2.29, <em>p</em> < 0.05) at 1 month, and more favorable hormonal changes (LH: 7.68 ± 1.15 vs. 8.92 ± 1.79 U/L; AMH: 6.40 ± 1.88 vs. 7.90 ± 2.39 ng/mL, <em>p</em> < 0.05) at 3 months. Favorable shifts in gut microbiota (increased <em>Faecalibacterium</em> and <em>Ruminococcus,</em> reduced <em>Collinsella</em>) were also observed. The combination reduced gastrointestinal side effects, and microbiota changes correlated with PCOS/IR biomarkers.</div><div><em>Conclusion: L. acidophilus</em> JYLA-126 supplementation may provide additional metabolic, hormonal, and gastrointestinal benefits to metformin therapy in PCOS, supporting its potential as a useful nutritional adjunct<em>.</em></div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"134 ","pages":"Article 107061"},"PeriodicalIF":4.0,"publicationDate":"2025-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145325573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-10-11DOI: 10.1016/j.jff.2025.107060
Yuyao Xie , Ning Ding , Jing Sun , Xiuli Jin , Xinhe Zhang , Ji Sun , Hongli Xu , Hao Bing , Lin Guan , Yiling Li , Ningning Wang
Metabolic dysfunction–associated steatotic liver disease (MASLD) is a growing health concern with limited treatment options. Here we show that oral betaine attenuates high-fat diet (HFD)–induced steatosis in mice and oleic acid–induced lipid accumulation in HepG2 cells. Betaine reduced body-weight gain, hepatic triglyceride content and Oil Red O staining, while restoring expression of miR-96-5p and lowering insulin-like growth factor 1 receptor (IGF1R). Gain-of-function of miR-96-5p decreased triglycerides and lipid droplets, and reporter assays confirmed direct targeting of IGF1R. Immunoblotting and immunofluorescence further showed reduced IGF1R expression in HFD-fed livers treated with betaine. These findings identify a betaine–miR-96-5p–IGF1R axis that mitigates hepatic steatosis and highlight betaine as a potential therapeutic strategy for MASLD.
代谢功能障碍相关的脂肪变性肝病（MASLD）是一个日益增长的健康问题，治疗方案有限。本研究表明，口服甜菜碱可以减轻高脂肪饮食（HFD）诱导的小鼠脂肪变性和油酸诱导的HepG2细胞脂质积累。甜菜碱降低体重增加、肝脏甘油三酯含量和油红O染色，同时恢复miR-96-5p的表达，降低胰岛素样生长因子1受体（IGF1R）。miR-96-5p的功能获得降低了甘油三酯和脂滴，报告者实验证实了IGF1R的直接靶向性。免疫印迹和免疫荧光进一步显示甜菜碱处理的hfd喂养肝脏中IGF1R表达降低。这些发现确定了甜菜碱- mir -96-5p - igf1r轴可以减轻肝脂肪变性，并强调甜菜碱是MASLD的潜在治疗策略。
{"title":"Oral betaine ameliorates high-fat diet-induced hepatic steatosis in mice by inducing miR-96-5p targeting IGF1R","authors":"Yuyao Xie , Ning Ding , Jing Sun , Xiuli Jin , Xinhe Zhang , Ji Sun , Hongli Xu , Hao Bing , Lin Guan , Yiling Li , Ningning Wang","doi":"10.1016/j.jff.2025.107060","DOIUrl":"10.1016/j.jff.2025.107060","url":null,"abstract":"<div><div>Metabolic dysfunction–associated steatotic liver disease (MASLD) is a growing health concern with limited treatment options. Here we show that oral betaine attenuates high-fat diet (HFD)–induced steatosis in mice and oleic acid–induced lipid accumulation in HepG2 cells. Betaine reduced body-weight gain, hepatic triglyceride content and Oil Red O staining, while restoring expression of miR-96-5p and lowering insulin-like growth factor 1 receptor (IGF1R). Gain-of-function of miR-96-5p decreased triglycerides and lipid droplets, and reporter assays confirmed direct targeting of IGF1R. Immunoblotting and immunofluorescence further showed reduced IGF1R expression in HFD-fed livers treated with betaine. These findings identify a betaine–miR-96-5p–IGF1R axis that mitigates hepatic steatosis and highlight betaine as a potential therapeutic strategy for MASLD.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"134 ","pages":"Article 107060"},"PeriodicalIF":4.0,"publicationDate":"2025-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145325611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-10-11DOI: 10.1016/j.jff.2025.107059
Xin Meng , Yiya Zhang , Fan Wang , Rui Mao , Xin Xiao , Zhili Deng , Qian Wang , Ying Tian , Hengshan Luo , Ji Li , San Xu
Phyllanthus emblica Linn. (PE), valued for its medicinal and nutritional properties, has been traditionally used to treat age-related disorders. However, its efficacy in preventing skin aging and the underlying mechanisms remain elusive. In the current investigation, we evaluated the anti-aging capacity of PE through in vitro and in vivo experiments, and employed molecular docking alongside experimental validation to probe the potential mechanisms. In vitro, PE enhanced the proliferative activity of senescent human dermal fibroblasts and suppressed the expression of senescence markers induced by both intrinsic and extrinsic factors. In vivo, PE treatment markedly inhibited wrinkle formation, reduced dermal thinning, and suppressed the upregulation of aging-related markers. Mechanistically, aquaporin-3 was identified as a potential target mediating the anti-aging effects of PE, with gallic acid likely being the key active component. Collectively, these findings support the potential of PE as a promising therapeutic agent for mitigating skin aging.
{"title":"Phyllanthus emblica Linn. Mitigates skin aging via targeting Aquaporin-3","authors":"Xin Meng , Yiya Zhang , Fan Wang , Rui Mao , Xin Xiao , Zhili Deng , Qian Wang , Ying Tian , Hengshan Luo , Ji Li , San Xu","doi":"10.1016/j.jff.2025.107059","DOIUrl":"10.1016/j.jff.2025.107059","url":null,"abstract":"<div><div><em>Phyllanthus emblica</em> Linn. (PE), valued for its medicinal and nutritional properties, has been traditionally used to treat age-related disorders. However, its efficacy in preventing skin aging and the underlying mechanisms remain elusive. In the current investigation, we evaluated the anti-aging capacity of PE through in vitro and in vivo experiments, and employed molecular docking alongside experimental validation to probe the potential mechanisms. In vitro, PE enhanced the proliferative activity of senescent human dermal fibroblasts and suppressed the expression of senescence markers induced by both intrinsic and extrinsic factors. In vivo, PE treatment markedly inhibited wrinkle formation, reduced dermal thinning, and suppressed the upregulation of aging-related markers. Mechanistically, aquaporin-3 was identified as a potential target mediating the anti-aging effects of PE, with gallic acid likely being the key active component. Collectively, these findings support the potential of PE as a promising therapeutic agent for mitigating skin aging.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"134 ","pages":"Article 107059"},"PeriodicalIF":4.0,"publicationDate":"2025-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145325612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-10-10DOI: 10.1016/j.jff.2025.107043
Chung-Hsiung Huang , Jia-Shan Liu , Yu-ChenS.H. Yang , Jhih-Wei Hsu , Shih-Yuan Hsu , Yang-Ching Chen
Maternal fish oil (FO) intake during pregnancy may reduce the risk of allergic rhinitis (AR) in offspring. This study integrated a human birth cohort (n = 804) and a mouse model to explore this relationship and underlying mechanisms. Maternal FO intake during pregnancy was associated with lower odds of AR symptoms (OR 0.90, 95 % CI 0.82–0.97, p = 0.01) and of physician-diagnosed AR or symptoms combined (OR 0.91, 95 % CI 0.83–0.99, p = 0.03). Gut microbiota profiling revealed increased abundance of beneficial bacteria such as Bifidobacteria and Lactobacilli. In mice, maternal FO intake suppressed House Dust Mite (HDM)-induced nasal inflammation, reduced IgE levels, and promoted expression of anti-inflammatory cytokines IL-10 and TGF-β. FO also reshaped gut microbial composition, suggesting an immunomodulatory role mediated via the microbiota. These findings indicate that maternal FO consumption may enhance immune tolerance and prevent early childhood AR.
母亲在怀孕期间摄入鱼油（FO）可能会降低后代患过敏性鼻炎（AR）的风险。本研究结合了人类出生队列（n = 804）和小鼠模型来探索这种关系及其潜在机制。孕妇孕期摄入FO与AR症状（OR 0.90, 95% CI 0.82-0.97, p = 0.01）以及医生诊断的AR或合并症状（OR 0.91, 95% CI 0.83-0.99, p = 0.03）的几率较低相关。肠道菌群分析显示有益细菌如双歧杆菌和乳酸杆菌的丰度增加。在小鼠中，母体摄入鱼油可抑制屋尘螨（HDM）引起的鼻腔炎症，降低IgE水平，促进抗炎细胞因子IL-10和TGF-β的表达。鱼油还重塑了肠道微生物组成，表明通过微生物群介导的免疫调节作用。这些发现表明，母体食用鱼油可能增强免疫耐受性并预防幼儿AR。
{"title":"Maternal Fish Oil Intake and Early Childhood Allergic Rhinitis: cohort associations supported by a mouse model","authors":"Chung-Hsiung Huang , Jia-Shan Liu , Yu-ChenS.H. Yang , Jhih-Wei Hsu , Shih-Yuan Hsu , Yang-Ching Chen","doi":"10.1016/j.jff.2025.107043","DOIUrl":"10.1016/j.jff.2025.107043","url":null,"abstract":"<div><div>Maternal fish oil (FO) intake during pregnancy may reduce the risk of allergic rhinitis (AR) in offspring. This study integrated a human birth cohort (<em>n</em> = 804) and a mouse model to explore this relationship and underlying mechanisms. Maternal FO intake during pregnancy was associated with lower odds of AR symptoms (OR 0.90, 95 % CI 0.82–0.97, <em>p</em> = 0.01) and of physician-diagnosed AR or symptoms combined (OR 0.91, 95 % CI 0.83–0.99, <em>p</em> = 0.03). Gut microbiota profiling revealed increased abundance of beneficial bacteria such as <em>Bifidobacteria</em> and <em>Lactobacilli</em>. In mice, maternal FO intake suppressed House Dust Mite (HDM)-induced nasal inflammation, reduced IgE levels, and promoted expression of anti-inflammatory cytokines IL-10 and TGF-β. FO also reshaped gut microbial composition, suggesting an immunomodulatory role mediated via the microbiota. These findings indicate that maternal FO consumption may enhance immune tolerance and prevent early childhood AR.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"134 ","pages":"Article 107043"},"PeriodicalIF":4.0,"publicationDate":"2025-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145263376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Immunosenescence, characterized by chronic inflammation and immune dysfunction, is a major contributor to age-related diseases. In this study, we investigated the effects of heat-killed Lentilactobacillus kefiri YRC2606 on immunosenescence. Aged C57BL/6 mice (16–18 months old) were fed YRC2606 (5 mg/day/mouse) for 8 weeks. YRC2606 treatment significantly increased the thymus index, reduced senescence marker expression in the spleen and kidney, and decreased proinflammatory cytokine levels in serum and tissues. Furthermore, phosphorylation of signal transducers and activators of transcription (STAT) 3, a key mediator of inflammation and senescence, was notably suppressed in the YRC2606 group. In vitro, YRC2606 suppressed lipopolysaccharide-induced interleukin (IL)-6 production and STAT3 activation in spleen cells. To our knowledge, this is the first report demonstrating that inactivated L. kefiri attenuates immunosenescence by regulating the IL-6/STAT3 pathway and alleviating thymic atrophy, highlighting its unique potential as a probiotic for age-related immune decline.
{"title":"Orally administered heat-killed Lentilactobacillus kefiri YRC2606 regulates immunosenescence in aged mice","authors":"Hiroka Sasahara , Haruka Aso , Fumie Niitsuma , Akihiro Masaki , Takayuki Arai , Shion Yamaguchi , Hyebin Jeong , Soyoka Ikeda , Hajime Tanimachi , Hikari Hayashida , Ikumi Fujioka , Kenji Uchida , Sachi Tanaka","doi":"10.1016/j.jff.2025.107053","DOIUrl":"10.1016/j.jff.2025.107053","url":null,"abstract":"<div><div>Immunosenescence, characterized by chronic inflammation and immune dysfunction, is a major contributor to age-related diseases. In this study, we investigated the effects of heat-killed <em>Lentilactobacillus kefiri</em> YRC2606 on immunosenescence. Aged C57BL/6 mice (16–18 months old) were fed YRC2606 (5 mg/day/mouse) for 8 weeks. YRC2606 treatment significantly increased the thymus index, reduced senescence marker expression in the spleen and kidney, and decreased proinflammatory cytokine levels in serum and tissues. Furthermore, phosphorylation of signal transducers and activators of transcription (STAT) 3, a key mediator of inflammation and senescence, was notably suppressed in the YRC2606 group. <em>In vitro</em>, YRC2606 suppressed lipopolysaccharide-induced interleukin (IL)-6 production and STAT3 activation in spleen cells. To our knowledge, this is the first report demonstrating that inactivated <em>L. kefiri</em> attenuates immunosenescence by regulating the IL-6/STAT3 pathway and alleviating thymic atrophy, highlighting its unique potential as a probiotic for age-related immune decline.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"134 ","pages":"Article 107053"},"PeriodicalIF":4.0,"publicationDate":"2025-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145263377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-10-10DOI: 10.1016/j.jff.2025.107065
Yan-Ping Li , Tian Liang , Yan-Bin Hao , Jian-Lin Shen , Qi Zou , Jing Huang , Jiang-Hong Luo , Er-Sheng Gong , Xiao-Yin Wang
This study aimed at investigating the effects of lotus seedpod polysaccharide (LSP) against human gastric cancer AGS cells and N-methyl-N′-nitro-N-nitrosoguanidine (MNNG)-induced gastric cancer rats. Moreover, the mechanism of LSP against AGS cells was explored by transcriptome and western blot analyses. Results indicated that LSP inhibited proliferation, migration and invasion of AGS cells, induced cell cycle arrest and apoptosis, increased levels of Ca2+, ROS and TGF-β1, and decreased ΔΨm and VEGF level. Moreover, LSP changed genes of AGS cells, especially downregulated the cell cycle pathway. CDC6, FZR1, CDC20, CDC27, MAD2L1, CDK1, CCNA2, ESPL1 and BUB1B were the hub targets for this pathway. LSP upregulated the protein expressions of FZR1 and CDC20, and downregulated the residual targets. Furthermore, LSP alleviated MNNG-induced gastric cancer in rats, and increased the levels of IgA and GSH in stomach. These findings indicate LSP possessed potential anti-gastric cancer activities, which supports the potential adjuvant application of LSP.
{"title":"In vitro and in vivo anti-gastric cancer activities of polysaccharide from lotus seedpod, a food by-product","authors":"Yan-Ping Li , Tian Liang , Yan-Bin Hao , Jian-Lin Shen , Qi Zou , Jing Huang , Jiang-Hong Luo , Er-Sheng Gong , Xiao-Yin Wang","doi":"10.1016/j.jff.2025.107065","DOIUrl":"10.1016/j.jff.2025.107065","url":null,"abstract":"<div><div>This study aimed at investigating the effects of lotus seedpod polysaccharide (LSP) against human gastric cancer AGS cells and <em>N</em>-methyl-N′-nitro-N-nitrosoguanidine (MNNG)-induced gastric cancer rats. Moreover, the mechanism of LSP against AGS cells was explored by transcriptome and western blot analyses. Results indicated that LSP inhibited proliferation, migration and invasion of AGS cells, induced cell cycle arrest and apoptosis, increased levels of Ca<sup>2+</sup>, ROS and TGF-β1, and decreased <em>ΔΨ</em>m and VEGF level. Moreover, LSP changed genes of AGS cells, especially downregulated the cell cycle pathway. CDC6, FZR1, CDC20, CDC27, MAD2L1, CDK1, CCNA2, ESPL1 and BUB1B were the hub targets for this pathway. LSP upregulated the protein expressions of FZR1 and CDC20, and downregulated the residual targets. Furthermore, LSP alleviated MNNG-induced gastric cancer in rats, and increased the levels of IgA and GSH in stomach. These findings indicate LSP possessed potential anti-gastric cancer activities, which supports the potential adjuvant application of LSP.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"134 ","pages":"Article 107065"},"PeriodicalIF":4.0,"publicationDate":"2025-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145263423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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