Pub Date : 2025-12-01Epub Date: 2025-11-19DOI: 10.1016/j.jff.2025.107103
Young Min Woo , Keun Woo Lee , Andre Kim
Liposomal delivery systems are utilized to enhance the bioavailability of nutrients. In this randomized, double-blind, two-period crossover clinical trial, healthy Korean adults (n = 20) were enrolled to assess the absorption of liposomal multinutrients compared to composition-matched non-liposomal comparators. Two distinct liposomal multinutrient formulations (LMN-1 and LMN-2) were compared with two corresponding non-liposomal multinutrient products (NLMN-1 and NLMN-2). Pharmacokinetic (PK) parameters—including C_max, T_max, and incremental area under the curve (iAUC)—were analyzed for vitamin B3, vitamin C, zinc, and iron. Vitamin C and zinc concentrations were significantly higher from 1 to 8 h and 0–6 h, respectively, while iron concentrations were elevated at most time points for the liposomal group. No clinically significant adverse events were observed during the study period. These findings indicate that liposomal multinutrients improve nutrient absorption and demonstrate good tolerability in humans. The results support the application of liposomal formulations in functional foods as a strategy to enhance nutrient delivery.
{"title":"Pharmacokinetics of liposomal multinutrients versus non-liposomal comparators in a randomized crossover trial","authors":"Young Min Woo , Keun Woo Lee , Andre Kim","doi":"10.1016/j.jff.2025.107103","DOIUrl":"10.1016/j.jff.2025.107103","url":null,"abstract":"<div><div>Liposomal delivery systems are utilized to enhance the bioavailability of nutrients. In this randomized, double-blind, two-period crossover clinical trial, healthy Korean adults (<em>n</em> = 20) were enrolled to assess the absorption of liposomal multinutrients compared to composition-matched non-liposomal comparators. Two distinct liposomal multinutrient formulations (LMN-1 and LMN-2) were compared with two corresponding non-liposomal multinutrient products (NLMN-1 and NLMN-2). Pharmacokinetic (PK) parameters—including C_max, T_max, and incremental area under the curve (iAUC)—were analyzed for vitamin B3, vitamin C, zinc, and iron. Vitamin C and zinc concentrations were significantly higher from 1 to 8 h and 0–6 h, respectively, while iron concentrations were elevated at most time points for the liposomal group. No clinically significant adverse events were observed during the study period. These findings indicate that liposomal multinutrients improve nutrient absorption and demonstrate good tolerability in humans. The results support the application of liposomal formulations in functional foods as a strategy to enhance nutrient delivery.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"135 ","pages":"Article 107103"},"PeriodicalIF":4.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145569123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-12-01Epub Date: 2025-11-22DOI: 10.1016/j.jff.2025.107109
Gabriela Ibieta , Jimena Ortiz-Sempértegui , J. Mauricio Peñarrieta , Javier A. Linares-Pastén
The development of bioactive peptides is of great interest in the food and pharmaceutical industries because of their positive impact on health. However, peptide's bioavailability depends on their stability along the gastrointestinal tract and their ability to be transported to targets located in peripheral organs, whose mechanisms are still poorly understood. However, several studies, mainly in vitro and in vivo, have shown antimicrobial, antitumoral, antihypertensive, anti-inflammatory, antidiabetic, and antioxidative effects. Indeed, some bioactive peptides are already on the global market. One of the current and future perspectives includes the development of novel bioactive peptides from food plant proteins. In this regard, some grains and seeds, such as quinoa, maize, cañihua and tarwi, which were domesticated in the Andes, are attracting global interest due to their high nutritional value and potential as a source of novel bioactive peptides. Tarwi stands out for its high protein content compared to other legumes.
{"title":"Bioactive peptides as functional food ingredients: Production, mechanisms of action, market trends, and future perspectives with emphasis on andean crops","authors":"Gabriela Ibieta , Jimena Ortiz-Sempértegui , J. Mauricio Peñarrieta , Javier A. Linares-Pastén","doi":"10.1016/j.jff.2025.107109","DOIUrl":"10.1016/j.jff.2025.107109","url":null,"abstract":"<div><div>The development of bioactive peptides is of great interest in the food and pharmaceutical industries because of their positive impact on health. However, peptide's bioavailability depends on their stability along the gastrointestinal tract and their ability to be transported to targets located in peripheral organs, whose mechanisms are still poorly understood. However, several studies, mainly <em>in vitro</em> and <em>in vivo</em>, have shown antimicrobial, antitumoral, antihypertensive, anti-inflammatory, antidiabetic, and antioxidative effects. Indeed, some bioactive peptides are already on the global market. One of the current and future perspectives includes the development of novel bioactive peptides from food plant proteins. In this regard, some grains and seeds, such as quinoa, maize, cañihua and tarwi, which were domesticated in the Andes, are attracting global interest due to their high nutritional value and potential as a source of novel bioactive peptides. Tarwi stands out for its high protein content compared to other legumes.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"135 ","pages":"Article 107109"},"PeriodicalIF":4.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145615210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-12-01Epub Date: 2025-10-28DOI: 10.1016/j.jff.2025.107074
Jianlong Lao , Junmei Li , Lin Tan , Shuping Yan , Zhaohai Wen , Yuan Qiu , Huiming Tan , Zengyuan Liu , Yanhong Yong , Xiaoxi Liu , Xingbin Ma , Xianghong Ju , Youquan Li
Intestinal inflammation in animals is a common intestinal disease, among which Crohn's disease (CD) and ulcerative colitis (UC) are the most common. However, probiotics are strain-specific, and there are significant differences in the mechanisms of action among different strains. In this study, Lacticaseibacillus paracasei MJ4 was isolated from the homemade yogurt of herdsmen in Qinghai Province, China. In vitro experiments demonstrated that MJ4 possesses excellent probiotic properties and safety profiles. In a murine model of colitis, MJ4 alleviated weight loss and colon shortening, and reduced the DAI score. After intervention with MJ4, the levels of pro-inflammatory factors (IL-1β, TNF-α, and IL-6) and histopathological scores decreased, whereas the level of IL-10 and the number of goblet cells increased significantly. Additionally, MJ4 rebalanced the intestinal flora composition in colitis mice by increasing the abundance of Faecalibaculum and Alistipes, and decreasing the abundance of Bacteroides, Escherichia_Shigella, and Akkermansia. Collectively, these findings indicate that L. paracasei MJ4 holds substantial potential for preventing colitis.
{"title":"Probiotic properties of Lacticaseibacillus paracasei MJ4 and its protective effect on mice with inflammatory bowel disease: A potential probiotic resource","authors":"Jianlong Lao , Junmei Li , Lin Tan , Shuping Yan , Zhaohai Wen , Yuan Qiu , Huiming Tan , Zengyuan Liu , Yanhong Yong , Xiaoxi Liu , Xingbin Ma , Xianghong Ju , Youquan Li","doi":"10.1016/j.jff.2025.107074","DOIUrl":"10.1016/j.jff.2025.107074","url":null,"abstract":"<div><div>Intestinal inflammation in animals is a common intestinal disease, among which Crohn's disease (CD) and ulcerative colitis (UC) are the most common. However, probiotics are strain-specific, and there are significant differences in the mechanisms of action among different strains. In this study, <em>Lacticaseibacillus paracasei</em> MJ4 was isolated from the homemade yogurt of herdsmen in Qinghai Province, China. In vitro experiments demonstrated that MJ4 possesses excellent probiotic properties and safety profiles. In a murine model of colitis, MJ4 alleviated weight loss and colon shortening, and reduced the DAI score. After intervention with MJ4, the levels of pro-inflammatory factors (IL-1β, TNF-α, and IL-6) and histopathological scores decreased, whereas the level of IL-10 and the number of goblet cells increased significantly. Additionally, MJ4 rebalanced the intestinal flora composition in colitis mice by increasing the abundance of <em>Faecalibaculum</em> and <em>Alistipes</em>, and decreasing the abundance of <em>Bacteroides</em>, <em>Escherichia_Shigella</em>, and <em>Akkermansia</em>. Collectively, these findings indicate that L. <em>paracasei</em> MJ4 holds substantial potential for preventing colitis.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"135 ","pages":"Article 107074"},"PeriodicalIF":4.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145374606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-12-01Epub Date: 2025-11-06DOI: 10.1016/j.jff.2025.107093
Qiang Yang , Ying Cai , Zhibo Wang , Sifan Guo , Shi Qiu , Aihua Zhang
Gardenia fructus (GF), a medicinal and edible herb with over 2,000 years of use, possesses diverse bioactive compounds including iridoids, crocins, and organic acids. Herein, we summarize the ethnomedicinal applications, phytochemistry, pharmacological activities, and safety concerns. GF exhibits a wide range of pharmacological activities, notably hepatoprotective, renoprotective, and neuroprotective effects. However, dose-dependent hepatotoxicity and nephrotoxicity - primarily linked to genipin, a metabolite of geniposide - pose significant safety challenges. Mechanisms involve oxidative stress, mitochondrial dysfunction, bile acid dysregulation, and inflammatory cascade activation. The field lacks sufficient clinical translation, comprehensive bioactivity analysis, and stringent quality control. Future research should prioritize elucidating molecular toxicity pathways, developing quality control benchmarks include geniposide, and advancing clinical trials to balance efficacy with safety. This positions GF as a sustainable resource for nutraceuticals and functional foods, necessitating rigorous safety assessments for rational utilization.
{"title":"From traditional medicinal use to functional food: The pharmacological activity, hepatotoxicity mechanism and detoxification strategies of gardenia fructus","authors":"Qiang Yang , Ying Cai , Zhibo Wang , Sifan Guo , Shi Qiu , Aihua Zhang","doi":"10.1016/j.jff.2025.107093","DOIUrl":"10.1016/j.jff.2025.107093","url":null,"abstract":"<div><div>Gardenia fructus (GF), a medicinal and edible herb with over 2,000 years of use, possesses diverse bioactive compounds including iridoids, crocins, and organic acids. Herein, we summarize the ethnomedicinal applications, phytochemistry, pharmacological activities, and safety concerns. GF exhibits a wide range of pharmacological activities, notably hepatoprotective, renoprotective, and neuroprotective effects. However, dose-dependent hepatotoxicity and nephrotoxicity - primarily linked to genipin, a metabolite of geniposide - pose significant safety challenges. Mechanisms involve oxidative stress, mitochondrial dysfunction, bile acid dysregulation, and inflammatory cascade activation. The field lacks sufficient clinical translation, comprehensive bioactivity analysis, and stringent quality control. Future research should prioritize elucidating molecular toxicity pathways, developing quality control benchmarks include geniposide, and advancing clinical trials to balance efficacy with safety. This positions GF as a sustainable resource for nutraceuticals and functional foods, necessitating rigorous safety assessments for rational utilization.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"135 ","pages":"Article 107093"},"PeriodicalIF":4.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145464265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-12-01Epub Date: 2025-11-08DOI: 10.1016/j.jff.2025.107096
Lu Zhou , Wen Jiang , Fei Pei , Yang Ji , Anxiang Su , Gaoxing Ma , Yimeng Zhang , Qiuhui Hu , Ning Ma
P.eryngii extruded rice (PEER) has been demonstrated to be a medium glycemic index food. To investigate its hypoglycemic potential, this study established a type 2 diabetic mouse model using streptozotocin (STZ) combined with a high-fat diet, followed by an 8 wk. dietary intervention. The results showed that, compared to the model control (MC) group, PEER intervention significantly lowered blood glucose, improved oral glucose tolerance (OGTT), reduced blood lipids (TC, TG, and LDL-C decreased by 18.22 %, 19.65 %, and 52.25 %, respectively) and insulin levels. In addition, PEER significantly upregulated the relative expression of IR, IRS-1, IRS-2, PI3K, and GSK-3β genes in the liver, altered the composition and structural proportion of gut microbiota in mice and notably increased the abundance of beneficial bacteria such as Akkermansia. In conclusion, PEER exhibited a significant hypoglycemic effect in type 2 diabetic mice, providing a theoretical basis for developing P. eryngii-based hypoglycemic products.
{"title":"Pleurotus eryngii extruded rice improved HFD/STZ-induced type 2 diabetic mice by activating PI3K/AKT signaling pathway and regulating gut microbiota","authors":"Lu Zhou , Wen Jiang , Fei Pei , Yang Ji , Anxiang Su , Gaoxing Ma , Yimeng Zhang , Qiuhui Hu , Ning Ma","doi":"10.1016/j.jff.2025.107096","DOIUrl":"10.1016/j.jff.2025.107096","url":null,"abstract":"<div><div><em>P.eryngii</em> extruded rice (PEER) has been demonstrated to be a medium glycemic index food. To investigate its hypoglycemic potential, this study established a type 2 diabetic mouse model using streptozotocin (STZ) combined with a high-fat diet, followed by an 8 wk. dietary intervention. The results showed that, compared to the model control (MC) group, PEER intervention significantly lowered blood glucose, improved oral glucose tolerance (OGTT), reduced blood lipids (TC, TG, and LDL-C decreased by 18.22 %, 19.65 %, and 52.25 %, respectively) and insulin levels. In addition, PEER significantly upregulated the relative expression of IR, IRS-1, IRS-2, PI3K, and GSK-3β genes in the liver, altered the composition and structural proportion of gut microbiota in mice and notably increased the abundance of beneficial bacteria such as Akkermansia. In conclusion, PEER exhibited a significant hypoglycemic effect in type 2 diabetic mice, providing a theoretical basis for developing <em>P. eryngii</em>-based hypoglycemic products.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"135 ","pages":"Article 107096"},"PeriodicalIF":4.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145464268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-12-01Epub Date: 2025-11-21DOI: 10.1016/j.jff.2025.107099
Yanan Gao , Na Zhang , Yi Li , Rong Zhang , Min Gao , Ya Zhang , Dan Jiang , Shuyan Liu
The aim of this study was to demonstrate the wound-healing activity of novel peptides derived from the eggshell membranes (ESM) of fresh chicken eggs and to explore the underlying mechanisms using integrated in silico and in vitro approaches. The ESM hydrolysates prepared by papain (PapH) exhibited the highest antioxidant and wound-healing activity. Three novel peptides, KDFPCPM (P1), DKDGPFRLC (P2), and LVEKGDVAF (P3), were identified from PapH via peptidomics and subsequently synthesized. These synthetic peptides also displayed excellent antioxidant and wounding-healing effects, with P1 surpassed the other peptides. Molecular docking results indicated that P1 could form stable bonds with Keap1, a key antioxidant target identified through proteomics. Furthermore, in vitro experiments utilizing the Nrf2 inhibitor ML385 revealed that P1 from ESM promotes cell migration by activating the Keap1-mediated Nrf2/HO-1 signaling pathway, thereby enhancing the cellular antioxidant defense system. Collectively, these findings provided new insights for the high-value utilization of discarded ESM.
{"title":"Identification and molecular mechanisms of novel wound healing potential peptides from eggshell membrane hydrolysates assisted by an integrated in silico and in vitro approach","authors":"Yanan Gao , Na Zhang , Yi Li , Rong Zhang , Min Gao , Ya Zhang , Dan Jiang , Shuyan Liu","doi":"10.1016/j.jff.2025.107099","DOIUrl":"10.1016/j.jff.2025.107099","url":null,"abstract":"<div><div>The aim of this study was to demonstrate the wound-healing activity of novel peptides derived from the eggshell membranes (ESM) of fresh chicken eggs and to explore the underlying mechanisms using integrated <em>in silico</em> and <em>in vitro</em> approaches. The ESM hydrolysates prepared by papain (PapH) exhibited the highest antioxidant and wound-healing activity. Three novel peptides, KDFPCPM (P1), DKDGPFRLC (P2), and LVEKGDVAF (P3), were identified from PapH <em>via</em> peptidomics and subsequently synthesized. These synthetic peptides also displayed excellent antioxidant and wounding-healing effects, with P1 surpassed the other peptides. Molecular docking results indicated that P1 could form stable bonds with Keap1, a key antioxidant target identified through proteomics. Furthermore, <em>in vitro</em> experiments utilizing the Nrf2 inhibitor ML385 revealed that P1 from ESM promotes cell migration by activating the Keap1-mediated Nrf2/HO-1 signaling pathway, thereby enhancing the cellular antioxidant defense system. Collectively, these findings provided new insights for the high-value utilization of discarded ESM.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"135 ","pages":"Article 107099"},"PeriodicalIF":4.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145569121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-12-01Epub Date: 2025-11-22DOI: 10.1016/j.jff.2025.107112
Nourhan M. Abd El-Aziz , Marwa R. Elbakatoshy , Ebtehal A. Farrage , Oscar Zannou , Reza Tahergorabi , Ayman M. Khalifah , Majid Aminzare , Mohamed G. Shehata
This study investigated the protective effects of Matcha extract against metabolic and organ issues caused by high-fat and high-sugar diets (HFSD) in rats. Matcha showed strong antioxidant activity, with phenolic and flavonoid contents measuring 238.25 mg/g and 60.81 mg/g, respectively. Rats fed on the HFSD gained more weight and had elevated levels of total cholesterol, LDL, triglycerides, and markers for liver and kidney stress, like ALT, AST, and creatinine. Matcha supplementation normalized the weight, cholesterol, LDL, ALT and creatinine. Antioxidant enzymes such as liver SOD were restored, and harmful substances like TBARS and NO were significantly decreased. Gene analysis revealed that beneficial genes were upregulated, while inflammatory markers were downregulated. Tissue analysis showed that Matcha helped protect liver and kidney structures from HFSD damage, reducing inflammation and cellular harm. Overall, the results highlight Matcha's promise as a dietary supplement for combating the metabolic and inflammatory effects of high-fat, high-sugar diets.
{"title":"Matcha mitigates metabolic and inflammatory disturbances induced by high-fat-sucrose diets in rats","authors":"Nourhan M. Abd El-Aziz , Marwa R. Elbakatoshy , Ebtehal A. Farrage , Oscar Zannou , Reza Tahergorabi , Ayman M. Khalifah , Majid Aminzare , Mohamed G. Shehata","doi":"10.1016/j.jff.2025.107112","DOIUrl":"10.1016/j.jff.2025.107112","url":null,"abstract":"<div><div>This study investigated the protective effects of Matcha extract against metabolic and organ issues caused by high-fat and high-sugar diets (HFSD) in rats. Matcha showed strong antioxidant activity, with phenolic and flavonoid contents measuring 238.25 mg/g and 60.81 mg/g, respectively. Rats fed on the HFSD gained more weight and had elevated levels of total cholesterol, LDL, triglycerides, and markers for liver and kidney stress, like ALT, AST, and creatinine. Matcha supplementation normalized the weight, cholesterol, LDL, ALT and creatinine. Antioxidant enzymes such as liver SOD were restored, and harmful substances like TBARS and NO were significantly decreased. Gene analysis revealed that beneficial genes were upregulated, while inflammatory markers were downregulated. Tissue analysis showed that Matcha helped protect liver and kidney structures from HFSD damage, reducing inflammation and cellular harm. Overall, the results highlight Matcha's promise as a dietary supplement for combating the metabolic and inflammatory effects of high-fat, high-sugar diets.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"135 ","pages":"Article 107112"},"PeriodicalIF":4.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145569126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The aim of this study was to evaluate the redox-related effects of extracts from four emblematic Greek wines – Xinomavro, Agiorgitiko, Assyrtiko, and Malagouzia. Their impact on cell viability and redox homeostasis was examined in HepG2, EA.hy926, and MKN45 cells. All extracts showed similar cytotoxic thresholds in EA.hy926 and MKN45 cells, while in HepG2 cells Xinomavro and Agiorgitiko wine extracts displayed lower thresholds than Assyrtiko and Malagouzia. Regarding redox modulation, Agiorgitiko enhanced antioxidant defenses in all cell lines and reduced lipid peroxidation in MKN45 cells. Xinomavro increased cellular antioxidant arsenal and mitigated lipid and protein oxidation. Assyrtiko and Malagouzia predominantly attenuated oxidative damage across all cell lines, with Assyrtiko further elevating antioxidant activity in EA.hy926 and MKN45 cells. Agiorgitiko and Malagouzia downregulated Nrf2 gene expression in EA.hy926 cells. Overall, these findings suggest that Greek wine extracts can beneficially modulate redox balance and represent promising sources of bioactive antioxidant compounds.
{"title":"The endemic Greek wines Χinomavro, Agiorgitiko, Αssyrtiko, and Malagouzia exert beneficial effects on human cells by upregulating the antioxidant defense mechanisms","authors":"Fotios Tekos , Periklis Vardakas , Zoi Skaperda , Paraskevi-Maria Nechalioti , Κorina Atsopardi , Kyriaki Xiftou , Anastasia Patouna , Maria Gkasdrogka , Aristidis S. Veskoukis , Antonis Giakountis , Konstantinos Poulas , Demetrios Kouretas","doi":"10.1016/j.jff.2025.107088","DOIUrl":"10.1016/j.jff.2025.107088","url":null,"abstract":"<div><div>The aim of this study was to evaluate the redox-related effects of extracts from four emblematic Greek wines – Xinomavro, Agiorgitiko, Assyrtiko, and Malagouzia. Their impact on cell viability and redox homeostasis was examined in HepG2, EA.hy926, and MKN45 cells. All extracts showed similar cytotoxic thresholds in EA.hy926 and MKN45 cells, while in HepG2 cells Xinomavro and Agiorgitiko wine extracts displayed lower thresholds than Assyrtiko and Malagouzia. Regarding redox modulation, Agiorgitiko enhanced antioxidant defenses in all cell lines and reduced lipid peroxidation in MKN45 cells. Xinomavro increased cellular antioxidant arsenal and mitigated lipid and protein oxidation. Assyrtiko and Malagouzia predominantly attenuated oxidative damage across all cell lines, with Assyrtiko further elevating antioxidant activity in EA.hy926 and MKN45 cells. Agiorgitiko and Malagouzia downregulated Nrf2 gene expression in EA.hy926 cells. Overall, these findings suggest that Greek wine extracts can beneficially modulate redox balance and represent promising sources of bioactive antioxidant compounds.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"135 ","pages":"Article 107088"},"PeriodicalIF":4.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145464264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-12-01Epub Date: 2025-11-13DOI: 10.1016/j.jff.2025.107098
Yina Huang , Yubin Chen , Jingyu He , Xunmei Shi , Yao He , Wu Sun , Chuanchao Wu , Kui Zhao , Di Xu
Polysaccharides derived from jujuba exhibit beneficial anti-ulcerative colitis effects, largely attributed to their potent antioxidant properties and other bioactivities. However, the mechanisms by which these polysaccharides from Ziziphus jujuba cv. Changzao modulate contribute to gut microbiota metabolism remain poorly understood. Therefore, this study aimed to instigate the effects of polysaccharides from Ziziphus jujuba cv. Changzao, sourced from various origins, on colitis. Our results demonstrated that LP-25 and FP-25 exhibited the most pronounced therapeutic efficacy, alleviating colitis symptoms and significantly reducing levels of pro-inflammation cytokines. Moreover, LP-25 and FP-25 promoted intestinal barrier repair by mitigating mucosal damage and upregulating the expression of ZO-1. Additionally, these polysaccharides favorably modulated gut microbiota composition-increasing the relative abundance of Firmicutes and Lactobacillus while decreasing that of Bacteroidetes. Furthermore, LP-25 and FP-25 may exert protective effects against colitis by regulating primary bile acid biosynthesis to protect colitis. Collectively, our findings suggest that LP-25 and FP-25 represent promising candidates for UC intervention, as their therapeutic effects are mediated through the regulation of gut microbiota and associated metabolic pathways.
{"title":"Polysaccharide from Ziziphus jujuba cv. Changzao prevented colitis in mice by regulating the inflammation-gut microbiota-metabolite axis","authors":"Yina Huang , Yubin Chen , Jingyu He , Xunmei Shi , Yao He , Wu Sun , Chuanchao Wu , Kui Zhao , Di Xu","doi":"10.1016/j.jff.2025.107098","DOIUrl":"10.1016/j.jff.2025.107098","url":null,"abstract":"<div><div>Polysaccharides derived from jujuba exhibit beneficial anti-ulcerative colitis effects, largely attributed to their potent antioxidant properties and other bioactivities. However, the mechanisms by which these polysaccharides from <em>Ziziphus jujuba</em> cv. Changzao modulate contribute to gut microbiota metabolism remain poorly understood. Therefore, this study aimed to instigate the effects of polysaccharides from <em>Ziziphus jujuba</em> cv. Changzao, sourced from various origins, on colitis. Our results demonstrated that LP-25 and FP-25 exhibited the most pronounced therapeutic efficacy, alleviating colitis symptoms and significantly reducing levels of pro-inflammation cytokines. Moreover, LP-25 and FP-25 promoted intestinal barrier repair by mitigating mucosal damage and upregulating the expression of ZO-1. Additionally, these polysaccharides favorably modulated gut microbiota composition-increasing the relative abundance of Firmicutes and <em>Lactobacillus</em> while decreasing that of <em>Bacteroidetes</em>. Furthermore, LP-25 and FP-25 may exert protective effects against colitis by regulating primary bile acid biosynthesis to protect colitis. Collectively, our findings suggest that LP-25 and FP-25 represent promising candidates for UC intervention, as their therapeutic effects are mediated through the regulation of gut microbiota and associated metabolic pathways.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"135 ","pages":"Article 107098"},"PeriodicalIF":4.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145517584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Colorectal cancer is the third most common cancer worldwide and preventative methods are currently desired. Oligonol derived from lychee is a polyphenol that reported to improve lifestyle-related diseases, but its effect on intestinal carcinogenesis has not been clarified. Thus, we investigated the effects of oligonol on Wnt/β-catenin signaling pathway in vitro and its effects on intestinal polyp development in vivo using Apc-mutant mice. As a result, oligonol treatment inhibited TCF/LEF reporter activity through non-phosphorylated β-catenin (active form) degradation in human colon cancer cells. Apc-mutant mice given oligonol for 8 weeks resulted in the suppression of intestinal polyp development along with inhibition of mRNA levels of c-Myc and LGR5, which are both regulated by the TCF/LEF transcription factor. Moreover, reduction of serum triglyceride levels was observed after oligonol administration. It is expected that polyphenols from lychee may become a new natural ingredient for cancer prevention in the future.
{"title":"Oligonol inhibits mouse intestinal tumorigenesis through Wnt signal regulation","authors":"Takahiro Hamoya, Shingo Miyamoto, Ryo Arita, Suzuko Ohsako, Michihiro Mutoh","doi":"10.1016/j.jff.2025.107100","DOIUrl":"10.1016/j.jff.2025.107100","url":null,"abstract":"<div><div>Colorectal cancer is the third most common cancer worldwide and preventative methods are currently desired. Oligonol derived from lychee is a polyphenol that reported to improve lifestyle-related diseases, but its effect on intestinal carcinogenesis has not been clarified. Thus, we investigated the effects of oligonol on Wnt/β-catenin signaling pathway <em>in vitro</em> and its effects on intestinal polyp development <em>in vivo</em> using <em>Apc</em>-mutant mice<em>.</em> As a result, oligonol treatment inhibited TCF/LEF reporter activity through non-phosphorylated β-catenin (active form) degradation in human colon cancer cells. <em>Apc</em>-mutant mice given oligonol for 8 weeks resulted in the suppression of intestinal polyp development along with inhibition of mRNA levels of <em>c-Myc</em> and <em>LGR5</em>, which are both regulated by the TCF/LEF transcription factor. Moreover, reduction of serum triglyceride levels was observed after oligonol administration. It is expected that polyphenols from lychee may become a new natural ingredient for cancer prevention in the future.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"135 ","pages":"Article 107100"},"PeriodicalIF":4.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145517587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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