Pub Date : 2025-03-26DOI: 10.1016/j.jff.2025.106775
Jia Wei , Gusonghan Maitiniyazi , Yin-Yin Gu , Jing-Wei Peng , Ye Sun , Shu-Fang Xia
Cydonia oblonga Mill. is a flavonoid-rich fruit and has potential applications in functional or nutritional foods. Herein, we examined the effects of hydroethanolic extract of Cydonia oblonga Mill. fruit (HECO, 600 mg/kg) on depressive-like behaviors in a mouse model of breast tumor. Flavonoids in HECO were determined by Ultra-Performance Liquid Chromatography-Mass Spectrometry. Depressive-like behaviors and hippocampal oxidative stress markers and inflammatory cytokines were measured. Colonic contents were collected for 16S rRNA sequencing. HECO was enriched with rutin, L-epicatechin, catechin and other flavonoids and significantly increased the time spent in the center of the open-field test and sucrose water consumption, and decreased immobility time in the tail suspension test without significant effect on tumor growth. HECO significantly increased hippocampal 5-hydroxytryptamine, brain-derived neurotrophic factor levels, glutathione peroxidase and superoxide dismutase activities, decreased hippocampal interleukin-6, interleukine-1β, tumor necrosis factor-α and malondialdehyde levels. In addition, HECO increased diversity of gut microbiota and improved intestinal barrier integrity. This study suggests that HECO is a potential candidate for breast cancer-related depression by regulating gut microbiota and inhibiting hippocampal oxidative stress and inflammation.
{"title":"Cydonia oblonga Mill. fruit extract attenuates depressive-like behaviors in a mouse model of breast tumor via regulating oxidative stress, inflammation, and gut microbiota","authors":"Jia Wei , Gusonghan Maitiniyazi , Yin-Yin Gu , Jing-Wei Peng , Ye Sun , Shu-Fang Xia","doi":"10.1016/j.jff.2025.106775","DOIUrl":"10.1016/j.jff.2025.106775","url":null,"abstract":"<div><div><em>Cydonia oblonga</em> Mill. is a flavonoid-rich fruit and has potential applications in functional or nutritional foods. Herein, we examined the effects of hydroethanolic extract of <em>Cydonia oblonga</em> Mill. fruit (HECO, 600 mg/kg) on depressive-like behaviors in a mouse model of breast tumor. Flavonoids in HECO were determined by Ultra-Performance Liquid Chromatography-Mass Spectrometry. Depressive-like behaviors and hippocampal oxidative stress markers and inflammatory cytokines were measured. Colonic contents were collected for 16S rRNA sequencing. HECO was enriched with rutin, L-epicatechin, catechin and other flavonoids and significantly increased the time spent in the center of the open-field test and sucrose water consumption, and decreased immobility time in the tail suspension test without significant effect on tumor growth. HECO significantly increased hippocampal 5-hydroxytryptamine, brain-derived neurotrophic factor levels, glutathione peroxidase and superoxide dismutase activities, decreased hippocampal interleukin-6, interleukine-1β, tumor necrosis factor-α and malondialdehyde levels. In addition, HECO increased diversity of gut microbiota and improved intestinal barrier integrity. This study suggests that HECO is a potential candidate for breast cancer-related depression by regulating gut microbiota and inhibiting hippocampal oxidative stress and inflammation.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"127 ","pages":"Article 106775"},"PeriodicalIF":3.8,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143715179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study evaluates the antihypertensive effects of Sacha Inchi meal protein hydrolysate (SIPH) in L-NAME-induced hypertensive rats. Male Sprague Dawley rats treated with L-NAME (40 mg/kg) received SIPH (100, 300, or 500 mg/kg), captopril (5 mg/kg), or a combination of captopril (2.5 mg/kg) and SIPH (500 mg/kg) for 5 weeks. Blood pressure was monitored weekly and verified via carotid artery cannulation. SIPH at 500 mg/kg, incombination with captopril, significantly reduced blood pressure, upregulated eNOS expression, alleviated renal and liver injury, enhanced sperm viability, and downregulated VCAM-1 expression. In HepG2 and 3 T3-L1 cells, SIPH mitigated oxidative stress, hepatic steatosis, and lipid accumulation. Together, these in vitro and in vivo findings suggest that SIPH could serve as a promising nutraceutical candidate for antihypertensive functional foods.
{"title":"Sacha inchi meal protein hydrolysate mitigates lipid accumulation and oxidative stress in HepG2 and 3 T3-L1 cells and synergistically enhances captopril's antihypertensive effects in L-NAME-induced hypertensive rats","authors":"Pakaporn Sa-nguanpong , Paweena Wetprasit , Anjaree Inchan , Chartchai Chaichana , Worasak Kaewkong , Natthawut Charoenphon , Kannika Adthapanyawanich , Krit Tantanarat , Worasit Tochampa , Khanitta Ruttarattanamongkol , Tippaporn Bualeong","doi":"10.1016/j.jff.2025.106772","DOIUrl":"10.1016/j.jff.2025.106772","url":null,"abstract":"<div><div>This study evaluates the antihypertensive effects of Sacha Inchi meal protein hydrolysate (SIPH) in L-NAME-induced hypertensive rats. Male Sprague Dawley rats treated with L-NAME (40 mg/kg) received SIPH (100, 300, or 500 mg/kg), captopril (5 mg/kg), or a combination of captopril (2.5 mg/kg) and SIPH (500 mg/kg) for 5 weeks. Blood pressure was monitored weekly and verified via carotid artery cannulation. SIPH at 500 mg/kg, incombination with captopril, significantly reduced blood pressure, upregulated eNOS expression, alleviated renal and liver injury, enhanced sperm viability, and downregulated VCAM-1 expression. In HepG2 and 3 T3-L1 cells, SIPH mitigated oxidative stress, hepatic steatosis, and lipid accumulation. Together, these in vitro and in vivo findings suggest that SIPH could serve as a promising nutraceutical candidate for antihypertensive functional foods.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"127 ","pages":"Article 106772"},"PeriodicalIF":3.8,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143697582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-03-26DOI: 10.1016/j.jff.2025.106749
Lianzhi Mao, Qunying Xie, Qiting Cheng, Wei Tang, Limei Mao
Eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) have been reported to improve insulin resistance (IR), but the mechanisms and differences remain unclear. Our results showed that EPA and DHA activated muscle IL-6 and improved IR in high-fat diet-fed mice. After knocking out muscle IL-6, the effect of EPA on improving systemic IR was abolished, but the effect of DHA was restored; however, neither EPA nor DHA improved muscle IR through activating AMPK. Furthermore, EPA and DHA activated IL-6 and improved palmitic acid-induced IR in C2C12 myotube cells. After silencing IL-6, neither EPA nor DHA improved IR through activating AMPK in myotube cells. Once AMPK was inhibited, the effect of EPA and DHA on improving IR was both abolished. Additionally, IL-6 activated AMPK and improved IR in myotube cells. In conclusion, EPA but not DHA improves systemic IR mainly due to attenuating muscle IR through IL-6/AMPK signaling pathway.
{"title":"EPA but not DHA improve systemic IR through activating muscle IL-6/AMPK pathway in high-fat diet-fed mice","authors":"Lianzhi Mao, Qunying Xie, Qiting Cheng, Wei Tang, Limei Mao","doi":"10.1016/j.jff.2025.106749","DOIUrl":"10.1016/j.jff.2025.106749","url":null,"abstract":"<div><div>Eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) have been reported to improve insulin resistance (IR), but the mechanisms and differences remain unclear. Our results showed that EPA and DHA activated muscle IL-6 and improved IR in high-fat diet-fed mice. After knocking out muscle IL-6, the effect of EPA on improving systemic IR was abolished, but the effect of DHA was restored; however, neither EPA nor DHA improved muscle IR through activating AMPK. Furthermore, EPA and DHA activated IL-6 and improved palmitic acid-induced IR in C2C12 myotube cells. After silencing IL-6, neither EPA nor DHA improved IR through activating AMPK in myotube cells. Once AMPK was inhibited, the effect of EPA and DHA on improving IR was both abolished. Additionally, IL-6 activated AMPK and improved IR in myotube cells. In conclusion, EPA but not DHA improves systemic IR mainly due to attenuating muscle IR through IL-6/AMPK signaling pathway.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"127 ","pages":"Article 106749"},"PeriodicalIF":3.8,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143697578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-03-26DOI: 10.1016/j.jff.2025.106767
Jianping Wu , Lidong Xie , Zhongyuan Qu , Hui Song , Xiangming Sun , Yang Hu , Wenlan Li
Inonotus obliquus (IO) is a kind of precious medicinal and edible fungi parasitized on birch trees in the cold zone, is widely recognized for its hypoglycemic properties. However, the material basis of its efficacy is unclear. This study investigated the hypoglycemic potential of polar fractions from IO and identified key active components. Using UPLC-Q-TOF/MS, we analyzed chemical compositions and evaluated the effects in a T2DM mouse model. IO-treated groups reduced FBG, OGTT-AUC, GSP, INS, HOMA-IR, TC, TG, LDL-c, ALT, and AST levels, and increased HDL-c. The IO-J fraction demonstrated a relatively prominent effect and liver protection in T2DM mice. Spectrum-effect relationship analysis revealed seven compounds correlated with hypoglycemia: Osmundacetone, Inonotusol A, Inonotusol C, Inonotusol F, Protocatechuic acid, Inonotusane F, and Inonotusone A. This research establishes the spectrum-effect relationship of IO fractions and provides a theoretical foundation for exploring the potential of IO as a hypoglycemic medicinal and edible fungi.
{"title":"Exploring the active components of hypoglycemic effect in different polar fractions of Inonotus obliquus based on spectrum-effect relationship","authors":"Jianping Wu , Lidong Xie , Zhongyuan Qu , Hui Song , Xiangming Sun , Yang Hu , Wenlan Li","doi":"10.1016/j.jff.2025.106767","DOIUrl":"10.1016/j.jff.2025.106767","url":null,"abstract":"<div><div><em>Inonotus obliquus</em> (IO) is a kind of precious medicinal and edible fungi parasitized on birch trees in the cold zone, is widely recognized for its hypoglycemic properties. However, the material basis of its efficacy is unclear. This study investigated the hypoglycemic potential of polar fractions from IO and identified key active components. Using UPLC-Q-TOF/MS, we analyzed chemical compositions and evaluated the effects in a T2DM mouse model. IO-treated groups reduced FBG, OGTT-AUC, GSP, INS, HOMA-IR, TC, TG, LDL-c, ALT, and AST levels, and increased HDL-c. The IO-J fraction demonstrated a relatively prominent effect and liver protection in T2DM mice. Spectrum-effect relationship analysis revealed seven compounds correlated with hypoglycemia: Osmundacetone, Inonotusol A, Inonotusol C, Inonotusol F, Protocatechuic acid, Inonotusane F, and Inonotusone A. This research establishes the spectrum-effect relationship of IO fractions and provides a theoretical foundation for exploring the potential of IO as a hypoglycemic medicinal and edible fungi.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"127 ","pages":"Article 106767"},"PeriodicalIF":3.8,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143697581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-03-26DOI: 10.1016/j.jff.2025.106754
Sonia Zodio , Gabriele Serreli , Anna Boronat , Rafael De la Torre , Maria Paola Melis , Monica Deiana
The aim of this investigation was to evaluate the protective effect of the main extra-virgin olive oil (EVOO) phenolic compounds, HT and Tyr, and their phase II metabolites, against the inflammatory response in HUVEC monolayers under hyperglycemic conditions. The alteration of endothelial barrier in HUVEC, treated with high glucose (HG, 30 mM) alone or together with EVOO phenols (1 μM), was evaluated through FITC-Dextran cell permeability test and the determination of TJ proteins occludin, zonulin and JAM-A level, in relation to redox-sensitive MAPK modulation, NLRP3 protein level and cytokines release. Obtained data showed that HG-induced increase of permeability through the alteration of TJ proteins, following the activation of upstream pathways involved in the inflammatory process such as p38, ERK1/2 and NLRP3, was reverted by pre-treatment with EVOO phenols and their metabolites, strengthening the hypothesis that these dietary compounds may exert a significant role in the maintenance of endothelial barrier integrity.
{"title":"Hydroxytyrosol, tyrosol and their phase II metabolites as inhibitors of endothelial cell inflammation induced by high glucose levels","authors":"Sonia Zodio , Gabriele Serreli , Anna Boronat , Rafael De la Torre , Maria Paola Melis , Monica Deiana","doi":"10.1016/j.jff.2025.106754","DOIUrl":"10.1016/j.jff.2025.106754","url":null,"abstract":"<div><div>The aim of this investigation was to evaluate the protective effect of the main extra-virgin olive oil (EVOO) phenolic compounds, HT and Tyr, and their phase II metabolites, against the inflammatory response in HUVEC monolayers under hyperglycemic conditions. The alteration of endothelial barrier in HUVEC, treated with high glucose (HG, 30 mM) alone or together with EVOO phenols (1 μM), was evaluated through FITC-Dextran cell permeability test and the determination of TJ proteins occludin, zonulin and JAM-A level, in relation to redox-sensitive MAPK modulation, NLRP3 protein level and cytokines release. Obtained data showed that HG-induced increase of permeability through the alteration of TJ proteins, following the activation of upstream pathways involved in the inflammatory process such as p38, ERK1/2 and NLRP3, was reverted by pre-treatment with EVOO phenols and their metabolites, strengthening the hypothesis that these dietary compounds may exert a significant role in the maintenance of endothelial barrier integrity.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"127 ","pages":"Article 106754"},"PeriodicalIF":3.8,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143697580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-03-26DOI: 10.1016/j.jff.2025.106768
Dominic Lomiwes , Alexander P. Kanon , Edward G. Walker , Nayer Ngametua , Janine M. Cooney , Dwayne A. Jensen , Duncan Hedderley , Kim Lo
The potential benefits of berryfruit phytonutrients on brain health in older adults remain underexplored. This randomised, placebo-controlled crossover investigated the effects of consuming Ārepa®, a powdered blackcurrant (BC) formula (7.8 mg anthocyanins/kg bodyweight of whole freeze-dried BC, 200 mg L-theanine and 100 mg pine bark extract), daily for 4 weeks on sleep quality and psychological wellbeing of healthy older adults (62 ± 6 years). Significant treatment × time interactions were observed for habitual sleep efficiency (p = 0.001; q = 0.015), with participants self-reporting improvements in sleep and general wellbeing. Bioavailability of BC anthocyanins, sarmentosin, and L-theanine was measurable after 4 weeks supplementation. No significant effects were found for psychological wellbeing or mood following 4 weeks of Ārepa® supplementation. These findings suggest the potential benefits of this BC formula to improve sleep quality in older adults.
{"title":"A blackcurrant powder supplement enriched with L-theanine and pine bark extract improves sleep quality in healthy older adults: Results from placebo controlled crossover study","authors":"Dominic Lomiwes , Alexander P. Kanon , Edward G. Walker , Nayer Ngametua , Janine M. Cooney , Dwayne A. Jensen , Duncan Hedderley , Kim Lo","doi":"10.1016/j.jff.2025.106768","DOIUrl":"10.1016/j.jff.2025.106768","url":null,"abstract":"<div><div>The potential benefits of berryfruit phytonutrients on brain health in older adults remain underexplored. This randomised, placebo-controlled crossover investigated the effects of consuming Ārepa®, a powdered blackcurrant (BC) formula (7.8 mg anthocyanins/kg bodyweight of whole freeze-dried BC, 200 mg L-theanine and 100 mg pine bark extract), daily for 4 weeks on sleep quality and psychological wellbeing of healthy older adults (62 ± 6 years). Significant treatment × time interactions were observed for habitual sleep efficiency (<em>p</em> = 0.001; q = 0.015), with participants self-reporting improvements in sleep and general wellbeing. Bioavailability of BC anthocyanins, sarmentosin, and L-theanine was measurable after 4 weeks supplementation. No significant effects were found for psychological wellbeing or mood following 4 weeks of Ārepa® supplementation. These findings suggest the potential benefits of this BC formula to improve sleep quality in older adults.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"127 ","pages":"Article 106768"},"PeriodicalIF":3.8,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143715180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-03-26DOI: 10.1016/j.jff.2025.106744
Zixuan Chen , Miaomiao Tong , Jiating Sun , Qinglin Wu , Zhuoran Li , Tianyi Lv , Xiaoxi Yin , Muqing Zhang , Li Li
Coix seed (CS), an agricultural crop often eaten as a staple food, has been used as a functional food treating ulcerative colitis (UC) recently. However, the underlying mechanism of CS on treating UC remains unclear. This study innovatively explored the preventive effects of CS on dextran sulfate sodium (DSS)-induced colitis in mice and its underlying mechanisms using lipid metabolomics and network pharmacology analysis. The results demonstrated that CS significantly reduced body weight loss, DAI scores, colonic damage and pro-inflammatory cytokines levels, such as TNF-α and IL-6, in UC mice. Additionally, CS reversed the DSS-induced downregulation of tight junction proteins, including ZO-1 and Occludin. Lipidomics results indicated a significant difference in lipid status between the normal and UC mice, which was reduced following CS treatment. Notably, levels of phosphatidylethanolamine (PE) and sphingomyelin (SM) were the most significantly altered lipids in UC mice after CS treatment. Furthermore, network pharmacology analysis indicated SRC, PPARG, PTGS2, MAPK1, MAPK3 and ESR1 as key targets for CS in alleviating UC inflammation. The enrichment analysis revealed that CS targeted functional modules associated with MAPK signaling and lipid metabolism. Further experimental validation, molecular docking and molecular dynamics simulation demonstrated that CS exerts significant protective effects through the Src/JNK MAPK and Nrf2/PPARγ signaling pathways. Overall, this study elucidated the potential of CS as a treatment for UC by regulating the lipid metabolism, which may be related to the Src/JNK MAPK and Nrf2/PPARγ signaling pathways. This strategy provides an important support for the utilization and development of CS as a functional food in human wellness, especially in ameliorating UC.
{"title":"Integrated lipid metabolomics and network pharmacology to investigate the potential mechanism of Coix seed in alleviating ulcerative colitis","authors":"Zixuan Chen , Miaomiao Tong , Jiating Sun , Qinglin Wu , Zhuoran Li , Tianyi Lv , Xiaoxi Yin , Muqing Zhang , Li Li","doi":"10.1016/j.jff.2025.106744","DOIUrl":"10.1016/j.jff.2025.106744","url":null,"abstract":"<div><div>Coix seed (CS), an agricultural crop often eaten as a staple food, has been used as a functional food treating ulcerative colitis (UC) recently. However, the underlying mechanism of CS on treating UC remains unclear. This study innovatively explored the preventive effects of CS on dextran sulfate sodium (DSS)-induced colitis in mice and its underlying mechanisms using lipid metabolomics and network pharmacology analysis. The results demonstrated that CS significantly reduced body weight loss, DAI scores, colonic damage and pro-inflammatory cytokines levels, such as TNF-α and IL-6, in UC mice. Additionally, CS reversed the DSS-induced downregulation of tight junction proteins, including ZO-1 and Occludin. Lipidomics results indicated a significant difference in lipid status between the normal and UC mice, which was reduced following CS treatment. Notably, levels of phosphatidylethanolamine (PE) and sphingomyelin (SM) were the most significantly altered lipids in UC mice after CS treatment. Furthermore, network pharmacology analysis indicated SRC, PPARG, PTGS2, MAPK1, MAPK3 and ESR1 as key targets for CS in alleviating UC inflammation. The enrichment analysis revealed that CS targeted functional modules associated with MAPK signaling and lipid metabolism. Further experimental validation, molecular docking and molecular dynamics simulation demonstrated that CS exerts significant protective effects through the Src/JNK MAPK and Nrf2/PPARγ signaling pathways. Overall, this study elucidated the potential of CS as a treatment for UC by regulating the lipid metabolism, which may be related to the Src/JNK MAPK and Nrf2/PPARγ signaling pathways. This strategy provides an important support for the utilization and development of CS as a functional food in human wellness, especially in ameliorating UC.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"127 ","pages":"Article 106744"},"PeriodicalIF":3.8,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143697579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-03-25DOI: 10.1016/j.jff.2025.106759
Haisheng Lin , Wen Wang , Fei Li , Jialong Gao , Zhongqin Chen , Wenhong Cao , Xiaoming Qin , Huina Zheng , Dean Ji , Hai Liu
Mantle tissue of pearl oyster is an underappreciated protein resource for functional peptides preparation. This study aimed to identify novel tyrosinase inhibitory peptides from collagen of Pinctada martensii mantle and to elucidate inhibition mechanism against tyrosinase. Collagen peptides of mantle were prepared by enzyme hydrolysis with animal protease. Two novel tyrosinase inhibitory peptides, Val-Leu-Ser-Val-Tyr (VLSLY) and Ile-Val-His-Arg-Lys-Cys-Phe (IVHRKCF), with IC50 values of 0.80 ± 0.03 mM and 1.51 ± 0.10 mM, respectively, were identified using LC-MS/MS methods combined with molecular docking. Moreover, the peptide IVHRKCF showed superior free radical scavenging ability. Hydrogen bonding and hydrophobic interactions were the main drivers of the interaction of peptides with tyrosinase, and IVHRKCF exhibited a more stable affinity. Lineweaver-Burk analysis results indicated that both peptides exhibited a mixed inhibition mechanism. This study provides new insights into the potential use of tyrosinase inhibitors in whitening foods and pharmaceuticals.
{"title":"Inhibitory mechanism of tyrosinase inhibitory peptides identified from the mantle of Pinctada martensii","authors":"Haisheng Lin , Wen Wang , Fei Li , Jialong Gao , Zhongqin Chen , Wenhong Cao , Xiaoming Qin , Huina Zheng , Dean Ji , Hai Liu","doi":"10.1016/j.jff.2025.106759","DOIUrl":"10.1016/j.jff.2025.106759","url":null,"abstract":"<div><div>Mantle tissue of pearl oyster is an underappreciated protein resource for functional peptides preparation. This study aimed to identify novel tyrosinase inhibitory peptides from collagen of <em>Pinctada martensii</em> mantle and to elucidate inhibition mechanism against tyrosinase. Collagen peptides of mantle were prepared by enzyme hydrolysis with animal protease. Two novel tyrosinase inhibitory peptides, Val-Leu-Ser-Val-Tyr (VLSLY) and Ile-Val-His-Arg-Lys-Cys-Phe (IVHRKCF), with IC50 values of 0.80 ± 0.03 mM and 1.51 ± 0.10 mM, respectively, were identified using LC-MS/MS methods combined with molecular docking. Moreover, the peptide IVHRKCF showed superior free radical scavenging ability. Hydrogen bonding and hydrophobic interactions were the main drivers of the interaction of peptides with tyrosinase, and IVHRKCF exhibited a more stable affinity. Lineweaver-Burk analysis results indicated that both peptides exhibited a mixed inhibition mechanism. This study provides new insights into the potential use of tyrosinase inhibitors in whitening foods and pharmaceuticals.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"127 ","pages":"Article 106759"},"PeriodicalIF":3.8,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143697577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-03-25DOI: 10.1016/j.jff.2025.106756
Lanlan Yang , Nian Yang , Yuanquan Ran , Huan Zhao , Qiong Hu , Yi Hong , Minyi Tian
Herein, the chemical constituents of Pittosporum glabratum leaf essential oil (EO) were analyzed, and its anticancer efficacy was explored in vitro and in vivo. Its primary phytochemicals were α-caryophyllene, (E)-β-farnesene, and germacrene D. It displayed significant cytotoxicity against cancerous A549 cells and lower cytotoxicity against noncancerous MRC-5 cells. It induced cell cycle arrest in the G1 phase by downregulating CDK2-cyclin E1/2 and CDK4-cyclin D3 complexes and upregulating p21, thereby inhibiting A549 cell proliferation. It triggered A549 cell apoptosis via the mitochondrial pathway, resulting in the ratio increase of Bax/Bcl-2, ΔΨm decrease, Cyt c release, caspase-9 and caspase-3 activation, and PARP cleavage. It blocked migration ability by decreasing MMP-2, MMP-9, and N-cadherin levels. Additionally, it effectively suppressed tumor growth by inducing apoptosis of transplanted tumor cells in nude mice. Hence, P. glabratum leaf EO has remarkable anticancer properties and has great potential as an anticancer drug in the pharmaceutical field.
{"title":"Pittosporum glabratum leaf (Shanzhi tea) essential oil: Chemical composition, anticancer activities and potential mechanisms in vitro and in vivo","authors":"Lanlan Yang , Nian Yang , Yuanquan Ran , Huan Zhao , Qiong Hu , Yi Hong , Minyi Tian","doi":"10.1016/j.jff.2025.106756","DOIUrl":"10.1016/j.jff.2025.106756","url":null,"abstract":"<div><div>Herein, the chemical constituents of <em>Pittosporum glabratum</em> leaf essential oil (EO) were analyzed, and its anticancer efficacy was explored <em>in vitro</em> and <em>in vivo</em>. Its primary phytochemicals were <em>α</em>-caryophyllene, (<em>E</em>)-<em>β</em>-farnesene, and germacrene D. It displayed significant cytotoxicity against cancerous A549 cells and lower cytotoxicity against noncancerous MRC-5 cells. It induced cell cycle arrest in the G1 phase by downregulating CDK2-cyclin E1/2 and CDK4-cyclin D3 complexes and upregulating p21, thereby inhibiting A549 cell proliferation. It triggered A549 cell apoptosis <em>via</em> the mitochondrial pathway, resulting in the ratio increase of Bax/Bcl-2, ΔΨm decrease, Cyt c release, caspase-9 and caspase-3 activation, and PARP cleavage. It blocked migration ability by decreasing MMP-2, MMP-9, and N-cadherin levels. Additionally, it effectively suppressed tumor growth by inducing apoptosis of transplanted tumor cells in nude mice. Hence, <em>P. glabratum</em> leaf EO has remarkable anticancer properties and has great potential as an anticancer drug in the pharmaceutical field.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"127 ","pages":"Article 106756"},"PeriodicalIF":3.8,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143683105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-03-25DOI: 10.1016/j.jff.2025.106758
Qi-Cong Chen , Chao Wu , Wei-Feng Cai , Qian Ni , Song-Xia Lin , Shao-Wei Zheng , Cui-Ping Jiang , Yan-Kui Yi , Qiang Liu , Chun-Yan Shen
Browning of white adipose tissue (WAT) and activation of brown adipose tissue (BAT) are novel strategies to prevent obesity. Eriocitrin, previously identified in blossom of Citrus aurantium L. var. amara Engl. (CAVA), has various beneficial effects. However, its role in browning of WAT and BAT activation remained unclear. In the current study, oleic acid-induced HepG2 cells and high fat-diet (HFD)-fed mice were developed and treated with eriocitrin. The results showed that eriocitrin significantly inhibited HepG2 cell steatosis, and prevented HFD-induced obesity and insulin resistance in mice. Eriocitrin also induced browning of WAT and activated BAT by increasing expression of thermogenic marker protein UCP1 and other brown/beige adipocyte markers. Further assays demonstrated that the anti-obesity effects of eriocitrin was probably achieved by regulating HSF1/PGC-1α pathway. These results suggested that eriocitrin might promote browning of WAT and activated BAT by activating HSF1/PGC-1α pathway, which was a promising candidate for developing weight-loss supplement.
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