Journal of Functional Foods最新文献_第2页

Curcumin as a multi-target nutritional intervention alleviates exercise-induced injuries from overtraining: intervention efficacy and mechanism of action 姜黄素作为多靶点营养干预减轻过度训练所致运动损伤的干预效果及作用机制

IF 4 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Journal of Functional Foods

Pub Date : 2026-01-23 DOI: 10.1016/j.jff.2026.107166

Senlin Lei , Shuyue Deng , Ruolan Shi , Yifu Ding , Xiaoan Chen , Xianhui Li

Curcumin (CUR), a natural polyphenol, exerts antioxidant, anti-inflammatory, and metabolic regulatory actions in exercise physiology and may help ameliorate overtraining-induced multi-organ dysfunction and delayed recovery states associated with overtraining syndrome (OTS). This review systematically summarizes the effects of CUR—across different doses, timing/duration regimens, and delivery systems—on exercise performance, functional recovery, and indices of muscle injury and multi-organ pathology in individuals with varying training status. Given that CUR is challenged by insufficient exposure due to low solubility, extensive first-pass metabolism, and rapid clearance, we introduce an “outcome-window–timing/duration–systemic exposure–outcome interpretation” framework to align systemic exposure with the temporal windows of recovery endpoints related to exercise-induced muscle damage (EIMD) (muscle function and muscle-damage–related indices). This framework is intended to improve the practical interpretability of dosing, supplementation timing, and delivery-system selection, while highlighting the decisive role of formulation type in exposure variability and cross-study comparability, as well as the moderating influence of training status on observable responses. Finally, future research directions were proposed, including optimized formulation and personalized supplementation strategies. Collectively, these insights support the development of multi-target, natural product–based interventions in sports medicine and encourage integrating phytochemicals into precision nutrition to promote recovery and optimize performance.

姜黄素（Curcumin， CUR）是一种天然多酚，在运动生理学中具有抗氧化、抗炎和代谢调节作用，可能有助于改善过度训练引起的多器官功能障碍和与过度训练综合征（OTS）相关的延迟恢复状态。这篇综述系统地总结了不同剂量、时间/持续时间方案和给药系统对不同训练状态个体的运动表现、功能恢复、肌肉损伤指标和多器官病理的影响。鉴于低溶解度、广泛的首过代谢和快速清除导致CUR暴露不足，我们引入了一个“结果窗口-时间/持续时间-系统暴露-结果解释”框架，将系统暴露与与运动诱导的肌肉损伤（EIMD）（肌肉功能和肌肉损伤相关指数）相关的恢复终点的时间窗口相结合。该框架旨在提高剂量、补充时间和给药系统选择的实际可解释性，同时强调配方类型在暴露变异性和交叉研究可比性中的决定性作用，以及训练状态对可观察反应的调节作用。最后，提出了今后的研究方向，包括优化配方和个性化补充策略。总的来说，这些见解支持运动医学中多目标、基于天然产品的干预措施的发展，并鼓励将植物化学物质整合到精确营养中，以促进恢复和优化表现。

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引用次数: 0

Fractionation, characterization, and gastrointestinal stability of anticancer and antioxidant peptides from sea cucumber (Holothuria scabra) hydrolysates 海参（Holothuria scabra）水解物中抗癌和抗氧化肽的分离、表征和胃肠道稳定性

IF 4 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Journal of Functional Foods

Pub Date : 2026-01-23 DOI: 10.1016/j.jff.2026.107173

Supansa Saiwong , Utoomporn Surayot , Narongchai Autsavapromporn , Charin Tachapan , Yuthana Phimolsiripol , Sang Guan You , Hui Hong , Sun Young Lim , Sutee Wangtueai

Sea cucumber hydrolysates with antioxidant and anticancer properties were prepared using papain hydrolysis. The protein hydrolysates were fractionated into peptide groups of >10 kDa (SCPH-I), 3–10 kDa (SCPH-II), 1–3 kDa (SCPH-III), and < 1 kDa (SCPH-IV) using ultrafiltration membranes. The antioxidant and anticancer properties of both crude and fractionated hydrolysates were evaluated. The SCPH-III fraction exhibited the most potent antioxidant properties, with scavenging activities against the 2,2-diphenyl-1-picryl hydrazyl (DPPH), 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), hydrogen peroxide (H₂O₂), and hydroxyl (OH) radicals of 87.96%, 78.48%, 39.39%, and 80.29%, respectively. The antiproliferative effect of the four fractions in the HepG2 cell model showed no significant difference (p > 0.05). The SCPH-III fraction was selected for peptide characterization using LC-MS/MS with de novo sequencing. Eighteen peptides were identified, among which two peptides demonstrated potent antioxidant and anticancer potential: Thr-Ser-Asp-Gly-Gln-Asn-Tyr-Leu-Leu-Leu-Lys and Phe-His-Val-Asp-Glu-Leu-Lys. The SCPH-III fraction induced significant morphological changes and apoptosis in HepG2 cells, with 87.30% of cells undergoing apoptosis at a 5 mg/mL concentration. Furthermore, the SCPH-III fraction was relatively stable under in vitro gastrointestinal digestion, showing a slight decrease in DPPH and OH radical scavenging activities, whereas the ABTS radical scavenging activity and anticancer activity increased significantly (p < 0.05).

采用木瓜蛋白酶水解法制备了具有抗氧化和抗癌特性的海参水解物。用超滤膜将蛋白水解产物分离成10 kDa （SCPH-I）、3-10 kDa （SCPH-II）、1 - 3 kDa （SCPH-III）和1 kDa （SCPH-IV）的肽组。对粗水解产物和分馏水解产物的抗氧化和抗癌性能进行了评价。SCPH-III部位对2,2-二苯基-1-吡啶酰肼（DPPH）、2,2 ' -氮基-双（3-乙基苯并噻唑-6-磺酸）、过氧化氢（H2O2）和羟基（OH）自由基的清除率分别为87.96%、78.48%、39.39%和80.29%，具有较强的抗氧化活性。四组分在HepG2细胞模型中的抗增殖作用差异无统计学意义（p > 0.05）。选择SCPH-III部分，采用LC-MS/MS与从头测序进行肽段表征。共鉴定出18条多肽，其中2条多肽具有较强的抗氧化和抗癌作用：thr - ser - asp - gly - gln - asn - tyrl - leu - leu - lys和pheh - his - val - asp - glu - leu - lys。SCPH-III组分在HepG2细胞中引起了明显的形态学改变和凋亡，在5 mg/mL浓度下，87.30%的细胞发生凋亡。体外胃肠消化作用下，SCPH-III组分相对稳定，对DPPH和OH自由基的清除能力略有下降，而对ABTS自由基的清除能力和抗癌能力显著提高（p < 0.05）。

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引用次数: 0

Gut microbiota and serum metabolites reveal the effects of chongqing tuo tea on glucose and lipid metabolism disorders in high-fat diet-fed mice 肠道菌群和血清代谢物揭示重庆二茶对高脂饮食小鼠糖脂代谢紊乱的影响

IF 4 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Journal of Functional Foods

Pub Date : 2026-01-21 DOI: 10.1016/j.jff.2026.107164

Rui Chang, Hongyu Luo, Shanmin Chen, Xue Chen, Ruiqi Duan, Ying Zhang, Yingfu Zhong

Obesity and its associated metabolic disorders constitute a major and growing global health challenge, creating an urgent need for safe and effective dietary interventions. Chongqing Tuo Tea, a traditionally compressed tea produced from summer-autumn leaves, has a distinctive chemical profile. Although it has been found that Chongqing Tuo Tea extract (TTE) can improve glucose and lipid metabolism, the underlying mechanisms, particularly those mediated via the gut-liver axis, are not well-understood. In the present study, TTE ameliorated hepatic lipid accumulation, attenuated pancreatic damage, improved the integrity of the intestinal barrier, reduced white adipose tissue deposition and promoted its browning, enhanced glucose tolerance, alleviated insulin resistance, and alleviated lipid metabolic disorders and hepatic oxidative stress in high-fat diet (HFD)-fed mice. TTE ameliorated disorders of glucose metabolism and reduced insulin resistance by activating the hepatic AMPK and PI3K/Akt signaling pathways. It restored the gut microbiota by reversing the Firmicutes/Bacteroidetes (F/B) ratio and promoting the proliferation of beneficial bacteria such as Oscillospira and Bacteroides, thereby increasing the production of short-chain fatty acids, particularly acetic, butyric, and propionic acids. Serum metabolomics and pathway enrichment analysis revealed that TTE significantly regulated metabolism-related pathways, including caffeine metabolism; central carbon metabolism in cancer; phenylalanine metabolism; phenylalanine, tyrosine, and tryptophan biosynthesis; ABC transporters; and glycerophospholipid metabolism. Several potential biomarkers of HFD-induced metabolic dysregulation were identified, including L-histidine, uridine monophosphate, shikimate-3-phosphate, arachidonic acid, and diaminopimelic acid. These findings highlight the potential of dietary TTE supplementation as a strategy for mitigating HFD-induced weight gain and metabolic abnormalities.

肥胖及其相关代谢紊乱是一项日益严重的重大全球健康挑战，迫切需要安全有效的饮食干预措施。重庆沱茶是一种传统的压缩茶，由夏秋的茶叶制成，具有独特的化学成分。虽然已经发现重庆两茶提取物（TTE）可以改善糖脂代谢，但其潜在机制，特别是通过肠-肝轴介导的机制尚不清楚。在本研究中，TTE改善了高脂饮食（HFD）喂养小鼠的肝脏脂质积累，减轻了胰腺损伤，改善了肠道屏障的完整性，减少了白色脂肪组织沉积并促进其褐变，增强了葡萄糖耐量，减轻了胰岛素抵抗，减轻了脂质代谢紊乱和肝脏氧化应激。TTE通过激活肝脏AMPK和PI3K/Akt信号通路改善糖代谢紊乱，降低胰岛素抵抗。它通过扭转厚壁菌门/拟杆菌门（F/B）的比例，促进有益菌如示波螺旋菌和拟杆菌的增殖，从而增加短链脂肪酸，特别是乙酸、丁酸和丙酸的产量，从而恢复肠道微生物群。血清代谢组学和途径富集分析显示，TTE显著调节代谢相关途径，包括咖啡因代谢；癌症中心碳代谢；苯丙氨酸代谢;苯丙氨酸、酪氨酸和色氨酸的生物合成；ABC转运蛋白;甘油磷脂代谢。研究人员确定了几种hfd诱导代谢失调的潜在生物标志物，包括l -组氨酸、单磷酸尿苷、莽草酸-3-磷酸、花生四烯酸和二氨基戊酸。这些发现强调了饮食中补充TTE作为减轻hfd引起的体重增加和代谢异常的策略的潜力。

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引用次数: 0

A synbiotic supplement reduces body fat and increases GLP-1 secretion in a pilot clinical study 合成补充剂减少体脂和增加GLP-1分泌在试点临床研究

IF 4 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Journal of Functional Foods

Pub Date : 2026-01-20 DOI: 10.1016/j.jff.2026.107171

Chiao-Wei Lin , Hsin-Bai Zou , Rou-An Chen , Chi-Shan Li , Chia-Ying Lin , Cheng-Chih Hsu

Obesity is closely associated with gut microbiota and their metabolites. We developed a multi-component synbiotic supplement that includes lychee polyphenols, Bifidobacterium longum, Bifidobacterium animalis, methionine, and other supportive ingredients such as prebiotics and postbiotics. A 5-week pilot clinical trial was conducted to evaluate its effects on body composition, metabolic markers, gut microbiota-derived metabolites, and GLP-1 secretion. The results showed significant reductions in body weight, fat mass, visceral fat, HbA1c, insulin resistance, and blood lipids. Metabolite analysis revealed increases in short-chain fatty acids and tryptophan metabolites, including acetic acid, isobutyric acid, isovaleric acid, serotonin, and indolelactic acid. GLP-1 secretion increased in both humans and mice, with a notable rise in Akkermansia muciniphila observed in mice given the synbiotic. These findings suggest that this synbiotic supplementation could serve as a potential approach for improving metabolic health and reducing obesity.

肥胖与肠道菌群及其代谢产物密切相关。我们开发了一种多组分合成补充剂，包括荔枝多酚、长双歧杆菌、动物双歧杆菌、蛋氨酸和其他支持性成分，如益生元和益生后。一项为期5周的先导临床试验评估了其对身体成分、代谢标志物、肠道微生物衍生代谢物和GLP-1分泌的影响。结果显示体重、脂肪量、内脏脂肪、糖化血红蛋白、胰岛素抵抗和血脂显著降低。代谢物分析显示短链脂肪酸和色氨酸代谢物增加，包括乙酸、异丁酸、异戊酸、血清素和吲哚乳酸。人和小鼠的GLP-1分泌均增加，在给予合成物的小鼠中观察到嗜粘液阿克曼氏菌的显著增加。这些发现表明，这种合成补充剂可以作为一种改善代谢健康和减少肥胖的潜在方法。

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引用次数: 0

Heat-killed Bifidobacterium longum LB-P23 ameliorates metabolic dysfunction in high-fat and high-sucrose diet-fed mice through gut microbiota modulation 热灭活长双歧杆菌LB-P23通过调节肠道菌群改善高脂高糖饮食小鼠的代谢功能障碍

IF 4 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Journal of Functional Foods

Pub Date : 2026-01-20 DOI: 10.1016/j.jff.2026.107160

Won Jun Kim , Mikyung Song , Hangsu Cho , Ahjin Kim , Jaeseok Shim , Kyoungsub Song

The gut microbiota plays a pivotal role in obesity and metabolic disorders. This study evaluated the anti-obesity effects of heat-killed Bifidobacterium longum LB-P23 in a high-fat, high-sucrose diet-induced obese mouse model. Mice received low (1 × 10⁸ TCC) or high (1 × 10⁹ TCC) doses of B. longum LB-P23 for 8 weeks. High-dose B. longum LB-P23 significantly reduced body weight gain, insulin resistance and hepatic steatosis, while enhancing thermogenesis, and gut barrier integrity by regulating genes related to insulin signaling, β-oxidation, thermogenesis, and tight junctions. It also alleviated diet-induced dysbiosis, reducing Parabacteroides, and Colidextribacter, while enriching Romboustia, whose abundance positively correlated with plasma IL-22, indicating activation of IL-22 signaling. In vitro, B. longum LB-P23 suppressed lipid accumulation in 3T3-L1 adipocytes. Collectively, heat-killed B. longum LB-P23 exerts multi-organ metabolic benefits through modulation of insulin signaling, thermogenesis, and gut–immune interactions, supporting its potential as a safe anti-obesity intervention.

肠道菌群在肥胖和代谢紊乱中起着关键作用。本研究在高脂肪、高糖饮食诱导的肥胖小鼠模型中评估了热灭活长双歧杆菌LB-P23的抗肥胖作用。小鼠接受低剂量（1 × 108 TCC）或高剂量（1 × 109 TCC）长梭菌LB-P23治疗8周。高剂量B. longum LB-P23可显著降低体重增加、胰岛素抵抗和肝脏脂肪变性，同时通过调节胰岛素信号、β-氧化、产热和紧密连接相关基因，增强产热和肠道屏障完整性。它还减轻了饮食引起的生态失调，减少了拟副杆菌和Colidextribacter，同时丰富了Romboustia，其丰度与血浆IL-22正相关，表明IL-22信号通路被激活。在体外实验中，长刺草LB-P23抑制3T3-L1脂肪细胞的脂质积累。综上所述，热杀伤长芽胞杆菌LB-P23通过调节胰岛素信号、产热和肠道免疫相互作用发挥多器官代谢益处，支持其作为安全抗肥胖干预的潜力。

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引用次数: 0

Anti-inflammatory effect of Cyanidin-3-O-lathyroside from Eleutherococcus sessiliflorus in LPS-induced RAW 264.7 cells 无梗棘球绦虫花青素-3- o -腺苷对lps诱导的RAW 264.7细胞的抗炎作用

IF 4 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Journal of Functional Foods

Pub Date : 2026-01-19 DOI: 10.1016/j.jff.2026.107169

Amylin Jay Eunice Cabuloy , Seo-Jin Lee , Don Carlo Batara , Heon-Woong Kim , Sung-Hak Kim , Jeong-Yong Cho

The anti-inflammatory effect of cyanidin-3-O-lathyroside was investigated in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages by examining cell morphology and quantifying pro-inflammatory cytokine expression by qPCR. Extracellular cytokine levels and the activity of inflammatory mediators were measured using ELISA and nitric oxide (NO) assays. Immunoblotting of the nuclear factor-kappa B (NF-κB) pathway targets and immunofluorescent staining of p65 were performed to elucidate the inhibitory mechanism. Treatment with cyanidin-3-O-lathyroside consistently reduced macrophage activation, as well as the mRNA and protein expression of cytokines (including tumor necrosis factor alpha (TNF-α), interleukin-1 beta (IL-1β), and interleukin-6 (IL-6)) and inflammatory mediators such as inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2). The phosphorylation of IκB-α and the nuclear translocation of p65 were also effectively inhibited by cyanidin-3-O-lathyroside at higher concentrations. Overall, the results demonstrate a dose-dependent suppression of LPS-induced inflammation in RAW 264.7 cells, supporting the potential of cyanidin-3-O-lathyroside as a natural anti-inflammatory agent.

本研究通过检测脂多糖（LPS）刺激下的RAW 264.7巨噬细胞的细胞形态和促炎细胞因子的定量表达，探讨了花青素-3- o-腺苷的抗炎作用。采用酶联免疫吸附试验和一氧化氮（NO）测定细胞外细胞因子水平和炎症介质活性。通过核因子κB （NF-κB）通路靶点的免疫印迹和p65的免疫荧光染色来阐明其抑制机制。用花青素-3- o -甲状腺苷治疗可持续降低巨噬细胞的活化，以及细胞因子(包括肿瘤坏死因子α (TNF-α)、白细胞介素-1β （IL-1β)和白细胞介素-6 (IL-6)）和炎症介质（如诱导型一氧化氮合酶（iNOS）和环氧化酶-2 (COX-2)） mRNA和蛋白的表达。高浓度的花青素-3- o -甲状腺苷也能有效抑制IκB-α的磷酸化和p65的核易位。总体而言，结果表明lps对RAW 264.7细胞诱导的炎症具有剂量依赖性抑制作用，支持了花青素-3- o - lagthyroside作为天然抗炎剂的潜力。

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引用次数: 0

Dietary polyphenols as functional food bioactives: Nuclear factor erythroid 2-related factor 2 (Nrf2)-mediated antioxidant and immunomodulatory mechanisms in combating bacterial infections 膳食多酚作为功能性食品生物活性物质：核因子-红细胞2相关因子2 （Nrf2）介导的抗氧化和免疫调节机制在对抗细菌感染中的作用

IF 4 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Journal of Functional Foods

Pub Date : 2026-01-18 DOI: 10.1016/j.jff.2026.107165

Nada Ahmed , Mohamed El-Fateh , Moussa S. Diarra , Xin Zhao

The rising threat of antimicrobial resistance (AMR) highlights the need for novel strategies to fight against microbial pathogens while strengthening host defenses. Dietary polyphenols abundant in fruits, vegetables, and food byproducts, could offer dual antimicrobial and host-protective effects. At the same time, nuclear factor erythroid 2-related factor 2 (Nrf2) activation emerges as one of key mechanisms that extend beyond antioxidant control to regulate cytokine production and innate immune signaling. This review discusses the underexplored role of polyphenol in activating Nrf2 as a signaling hub that coordinates host immune responses against bacterial infection. Despite bioavailability hurdles, polyphenols could offer a promise direction for functional foods-based strategies in infection control.

抗菌素耐药性（AMR）的威胁日益严重，这表明需要新的策略来对抗微生物病原体，同时加强宿主的防御。富含水果、蔬菜和食品副产品的膳食多酚具有抗菌和保护宿主的双重作用。与此同时，核因子红细胞2相关因子2 （Nrf2）的激活成为超越抗氧化控制，调节细胞因子产生和先天免疫信号传导的关键机制之一。这篇综述讨论了多酚在激活Nrf2作为协调宿主免疫反应对抗细菌感染的信号中枢中的作用。尽管存在生物利用度障碍，但多酚可以为基于功能食品的感染控制策略提供一个有希望的方向。

引用次数: 0

Sparalide B from Sparassis latifolia inhibits early adipogenesis in 3T3-L1 adipocytes via β-catenin-dependent downregulation of PPARγ and C/EBPα 疏树苷B通过β-catenin依赖性下调PPARγ和C/EBPα抑制3T3-L1脂肪细胞的早期脂肪形成

IF 4 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Journal of Functional Foods

Pub Date : 2026-01-17 DOI: 10.1016/j.jff.2026.107167

Hyeok Jin Choi , Jeong Won Choi , So Jung Park , Gyeong Eun Im , Youngki Park , Kyoung Tae Lee , Jin Boo Jeong

Sparalide B, a phthalide isolated from Sparassis latifolia (S. latifolia), was structurally assigned as 4,5,7-trihydroxy-6-ethoxyphthalide by high-resolution electrospray ionization mass spectrometry (HR-ESI-MS) and nuclear magnetic resonance (NMR) (¹H, ¹³C, heteronuclear multiple bond correlation (HMBC)) analyses. In 3T3-L1 adipocytes, sparalide B (2.5–20 μg/mL) significantly reduced lipid droplet accumulation in a dose-dependent manner, as quantified by Oil Red O staining at concentrations that did not affect cell viability. Western blotting showed decreased expression of adipogenic transcription factors (PPARγ, C/EBPα) and lipid droplet-associated/lipolytic proteins (perilipin-1, ATGL, HSL), alongside increased levels of thermogenesis/energy-metabolism markers (UCP2, PGC-1α, p-AMPK, AMPK). Stage-restricted treatments revealed that efficacy was confined to the early differentiation window (D0-D2), with little effect during D2-D4 or D4-D8. Mechanistically, sparalide B elevated β-catenin during D0-D2; β-catenin knockdown abrogated the compound's suppression of PPARγ/C/EBPα and its inhibition of lipid accumulation. These findings indicate that sparalide B targets early adipogenesis through β-catenin-dependent signaling to suppress lipid formation, highlighting this natural phthalide as a promising anti-obesity lead.

通过高分辨率电喷雾质谱（HR-ESI-MS）和核磁共振（NMR）（1H, 13C，异核多键相关（HMBC））分析，从菝葜（S. latifolia）中分离得到邻苯酞类化合物Sparalide B，结构为4,5,7-三羟基-6-乙氧基邻苯酞。在3T3-L1脂肪细胞中，经油红O染色，在不影响细胞活力的浓度下，稀疏胺B （2.5-20 μg/mL）以剂量依赖性方式显著降低脂滴积累。Western blotting显示，脂肪生成转录因子（PPARγ、C/EBPα）和脂滴相关/脂解蛋白（perilipin-1、ATGL、HSL）的表达下降，产热/能量代谢标志物（UCP2、PGC-1α、p-AMPK、AMPK）水平升高。限期治疗显示，疗效仅限于早期分化窗口（D0-D2），在D2-D4或D4-D8期间效果不大。在机制上，疏肽B在D0-D2期间升高β-catenin；β-catenin的敲除消除了该化合物对PPARγ/C/EBPα的抑制作用及其对脂质积累的抑制作用。这些发现表明，sparalide B通过β-连环蛋白依赖的信号传导抑制脂质形成，靶向早期脂肪形成，突出了这种天然邻苯酞作为一种有希望的抗肥胖先导物。

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引用次数: 0

Effects of consumption of selenium biofortified milk on antioxidant and inflammatory biomarkers in children with autism spectrum disorder: A controlled, single-blind intervention study 食用硒生物强化牛奶对自闭症谱系障碍儿童抗氧化和炎症生物标志物的影响：一项对照单盲干预研究

IF 4 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Journal of Functional Foods

Pub Date : 2026-01-16 DOI: 10.1016/j.jff.2026.107161

Marta Liliane de Vasconcelos , Fábio da Veiga Ued , Marcia Delgado da Cruz Gomes , Luiz Fernando Costa e Silva , Giovana Fumes Ghantous , Arlindo Saran Netto , João Alberto Negrão , Jeremy Paul Hill , Ana Maria Centola Vidal

Children with Autism Spectrum Disorder (ASD) often exhibit food selectivity, which may be associated with inadequate Selenium (Se) intake and imbalances in serum levels of enzymatic and non-enzymatic antioxidants as well as inflammatory cytokines. Several studies suggest an association between autism and increased oxidative stress. However, clinical studies in this population remain scarce due to methodological challenges, as do studies assessing Se status and the effects of Se biofortified foods. In this context, the present study aimed to quantify serum levels of Se, zinc (Zn), vitamin E (VitE), antioxidant enzymes, and pro-inflammatory cytokines in children with ASD before and after dietary intervention with Se biofortified cow's milk. Seventeen children with ASD aged between 3 and 10 years were selected and allocated into a conventional milk (n = 8), and the Se biofortification group (n = 9), which received cow's milk containing 190 μg/L of Se for 12 weeks (84 days). Serum levels of enzymatic and non-enzymatic antioxidants and gene expression of pro-inflammatory cytokines were measured at baseline and the end of the study. Following the intervention, serum Se levels were significantly higher in the biofortified group (62.97 vs 102.82 μg/dL, p < 0.05). No significant differences were observed in antioxidant enzyme expression; although not statistically significant, greater glutathione peroxidase 1 (GPx1) and catalase (CAT) levels were observed post-intervention, as well as a tendency toward lower VitE and Zn levels. Among the measured pro-inflammatory cytokines, only interleukin 8 (IL-8) significantly increased. Tumor necrosis factor-alpha (TNF-α) and interferon-gamma (IFN-γ) showed a positive correlation with antioxidant enzymes (p < 0.05). These findings suggest that Se biofortification of milk may serve as a potential nutritional strategy for children with ASD.

患有自闭症谱系障碍（ASD）的儿童通常表现出食物选择性，这可能与硒（Se）摄入不足、血清酶促和非酶促抗氧化剂以及炎症细胞因子水平失衡有关。几项研究表明，自闭症与氧化应激增加有关。然而，由于方法学上的挑战，对这一人群的临床研究仍然很少，评估硒状况和硒生物强化食品效果的研究也是如此。在此背景下，本研究旨在量化ASD儿童在硒生物强化牛奶饮食干预前后的血清硒、锌（Zn）、维生素E （VitE）、抗氧化酶和促炎细胞因子水平。选取17名年龄在3 ~ 10岁之间的ASD儿童，将其分为常规牛奶组（n = 8）和硒生物强化组（n = 9），他们连续12周（84天）饮用含硒190 μg/L的牛奶。在基线和研究结束时测量血清酶促和非酶促抗氧化剂水平以及促炎细胞因子的基因表达。干预后，生物强化组血清硒水平显著高于对照组（62.97 vs 102.82 μg/dL, p < 0.05）。抗氧化酶表达差异无统计学意义；虽然没有统计学意义，但干预后观察到谷胱甘肽过氧化物酶1 （GPx1）和过氧化氢酶（CAT）水平升高，以及VitE和Zn水平降低的趋势。在检测的促炎细胞因子中，只有白细胞介素8 （IL-8）显著升高。肿瘤坏死因子-α (TNF-α)和干扰素-γ (IFN-γ)与抗氧化酶呈正相关（p < 0.05）。这些发现表明，牛奶中的硒生物强化可能是自闭症儿童的一种潜在营养策略。

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引用次数: 0

A polysaccharide from Artocarpus heterophyllus Lam. pulp ameliorates oxidative stress and lipid accumulation in HepG2 cells 一种从石竹中提取的多糖。牙髓改善HepG2细胞的氧化应激和脂质积累

IF 4 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Journal of Functional Foods

Pub Date : 2026-01-14 DOI: 10.1016/j.jff.2026.107163

Panjie Xu , Benyan Bai , Huawei Chen , Yanjun Zhang , Zhiyuan Ma , Gang Wu , Yan Zhou , Kexue Zhu

Oxidative stress and hepatic lipid accumulation are two key contributors to the development of non-alcoholic fatty liver disease (NAFLD). The present research sought to examine the effect of a polysaccharide from Artocarpus heterophyllus Lam. pulp (JFP-Ps) on alleviating hydrogen peroxide (H₂O₂)-induced cellular oxidative stress, and attenuating oleic acid (OA)-induced lipid accumulation in HepG2 cells, respectively. Results showed that JFP-Ps considerably lower intracellular ROS and MDA concentrations, elevate the activities of CAT, SOD, GSH-Px, and T-AOC. Furthermore, JFP-Ps exhibited antioxidant effects by up-regulating Nrf2, NQO1 and HO-1 expression, and down-regulating ERK and P38 expression in H₂O₂-treated HepG2 cells. Western blot determination indicated that JFP-Ps up-regulated the expression of Nrf2/HO-1/NQO1 in the Nrf2 pathway and down-regulated the expression of PERK and p-P38 in the MAPK pathway. JFP-Ps decreased the TG and TC contents, reduced the relative area of lipid droplets, and restored OA-elicited lipid metabolism disorders in HepG2 cells. The lipid-lowering impact of JFP-Ps is facilitated through down-regulating PPARγ/SREBP1/HMGCR/FAS, and upregulating of CPT1A/CYP7A1 expressions. These results could offer valuable insights for the potential application of JFP-Ps in NAFLD treatment.

氧化应激和肝脏脂质积累是非酒精性脂肪性肝病（NAFLD）发展的两个关键因素。摘要本研究旨在探讨一种从石竹中提取的多糖的作用。JFP-Ps分别减轻过氧化氢（H2O2）诱导的细胞氧化应激和油酸（OA）诱导的HepG2细胞脂质积累。结果表明，JFP-Ps显著降低了细胞内ROS和MDA浓度，提高了CAT、SOD、GSH-Px和T-AOC的活性。此外，JFP-Ps通过上调h2o2处理的HepG2细胞中Nrf2、NQO1和HO-1的表达，下调ERK和P38的表达，表现出抗氧化作用。Western blot检测显示，JFP-Ps上调Nrf2通路中Nrf2/HO-1/NQO1的表达，下调MAPK通路中PERK和p-P38的表达。JFP-Ps降低了TG和TC含量，减少了脂滴的相对面积，恢复了oa引起的HepG2细胞脂质代谢紊乱。JFP-Ps的降脂作用是通过下调PPARγ/SREBP1/HMGCR/FAS，上调CPT1A/CYP7A1表达来实现的。这些结果为JFP-Ps在NAFLD治疗中的潜在应用提供了有价值的见解。

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