A copious amount of rigid Aloe vera leaf rind (AVLR) has been produced from the aloe gel processing industries are majorly disposed as wastes since it has no commercial value. The cell wall compositional analysis revealed that significant quantity of cellulose (46% ± 0.76, w/w) and hemicellulose (18.5% ± 0.24, w/w) which justifies as potent source for bioethanol production. However, high lignin content (13.95% ± 0.45, w/w) hinders depolymerization of polysaccharides into fermentable sugars and subsequent fermentation for ethanol production. In the present study, microwave-assisted alkali (MAA) pretreatment of AVLR biomass was carried out by varying the power level (160 W, 320 W and 480 W) which showed a maximum delignification (66.38%) at 320 W. Scanning Electron Microscope (SEM), Fourier-transform infrared spectroscopy (FTIR) and X-ray powder diffraction (XRD) based characterization were performed to study the extent of delignification in AVLR biomass. The Gas Chromatography-Mass Spectrometry (GC-MS) analysis was performed for the liquid hydrolysate obtained after MAA pretreatment at 320 W indicated that the hydrolysate contained more of oxidized phenolic hydrocarbons that can be potentially utilized for other value-added product synthesis. A comparison of saccharification efficiency was performed using two different cellulase producers namely Aspergillus niger and Aspergillus sp. A maximum saccharification of 68.5% ± 0.34 was achieved by Aspergillus sp., that was 2.8% higher on comparing with untreated AVLR biomass. This indicates the feasibility of MAA pretreatment for AVLR biomass in order to improve the accessibility of fermentable sugars available for ethanol production.
由于芦荟凝胶加工工业生产了大量的硬质芦荟叶皮(AVLR)，由于它没有商业价值，因此主要作为废物处理。细胞壁成分分析显示，纤维素(46%±0.76,w/w)和半纤维素(18.5%±0.24,w/w)含量显著，证明其是生物乙醇生产的有效来源。然而，高木质素含量(13.95%±0.45,w/w)阻碍了多糖解聚成可发酵糖并随后发酵生产乙醇。采用不同功率(160 W、320 W和480 W)对AVLR生物质进行微波辅助碱(MAA)预处理，结果表明，在320 W时脱木率最高(66.38%)。采用扫描电镜(SEM)、傅里叶变换红外光谱(FTIR)和x射线粉末衍射(XRD)等表征方法研究了AVLR生物质脱木质素的程度。采用气相色谱-质谱(GC-MS)对320 W MAA预处理后的水解液进行分析，结果表明，水解液中含有较多的氧化酚类化合物，可用于其他增值产品的合成。比较了两种不同的纤维素酶产生菌黑曲霉和曲霉的糖化效率。曲霉的最大糖化率为68.5%±0.34，比未处理的AVLR生物量高2.8%。这表明MAA预处理AVLR生物质以提高乙醇生产可发酵糖的可及性是可行的。
{"title":"A hybrid pretreatment strategy for delignification of Aloe vera processing waste and its effectiveness towards saccharification","authors":"Rajeswari Gunasekaran, S. Jacob","doi":"10.5455/jabet.2022.d134","DOIUrl":"https://doi.org/10.5455/jabet.2022.d134","url":null,"abstract":"A copious amount of rigid Aloe vera leaf rind (AVLR) has been produced from the aloe gel processing industries are majorly disposed as wastes since it has no commercial value. The cell wall compositional analysis revealed that significant quantity of cellulose (46% ± 0.76, w/w) and hemicellulose (18.5% ± 0.24, w/w) which justifies as potent source for bioethanol production. However, high lignin content (13.95% ± 0.45, w/w) hinders depolymerization of polysaccharides into fermentable sugars and subsequent fermentation for ethanol production. In the present study, microwave-assisted alkali (MAA) pretreatment of AVLR biomass was carried out by varying the power level (160 W, 320 W and 480 W) which showed a maximum delignification (66.38%) at 320 W. Scanning Electron Microscope (SEM), Fourier-transform infrared spectroscopy (FTIR) and X-ray powder diffraction (XRD) based characterization were performed to study the extent of delignification in AVLR biomass. The Gas Chromatography-Mass Spectrometry (GC-MS) analysis was performed for the liquid hydrolysate obtained after MAA pretreatment at 320 W indicated that the hydrolysate contained more of oxidized phenolic hydrocarbons that can be potentially utilized for other value-added product synthesis. A comparison of saccharification efficiency was performed using two different cellulase producers namely Aspergillus niger and Aspergillus sp. A maximum saccharification of 68.5% ± 0.34 was achieved by Aspergillus sp., that was 2.8% higher on comparing with untreated AVLR biomass. This indicates the feasibility of MAA pretreatment for AVLR biomass in order to improve the accessibility of fermentable sugars available for ethanol production.","PeriodicalId":36275,"journal":{"name":"Journal of Advanced Biotechnology and Experimental Therapeutics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70796521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract Infection with the hepatitis B virus (HBV) continues to be a hazard for public health across the globe. Chronic hepatitis, cirrhosis, and hepatocellular carcinoma are all possible outcomes (HCC). It is obvious that certain patients with chronic hepatitis B (CHB) viral infection developed HCC, while other under almost similar circumstances do not. To inspect the possible there being a link between three single nucleotide gene polymorphisms (SNPs) in GzmB genes with the development of HCC. A total of 85 patients diagnosed with CHB participated in this research (40 patients with HCC and 45 patients without HCC). Three SNPs in GzmB gene (rs7144366, rs8192917 and rs2236338) were genotypes using restriction fragment length polymorphism (RFLP). The haplotype blocks derived from the three SNPs were assembled, and the linkage disequilibrium (LD) between the SNPs was determined using the SHEsis software. The homozygous mutant genotype (CC) was shown to be significantly more common in patients with HCC (27.5 %) than in those without HCC (11.11 %) (OR= 3.93, 95 percent CI=1.13-13.62, p=0.031). At allelic level, the mutant allele (C) was more frequent in patients with than those without HCC (46.25% vs. 26.67%) with a significant deviation (OR=2.36, 95%CI= 1.25- 4.49, p= 0.008). the haplotype block CCG was more common among patients with HCC (26.25%) than those without HCC (12.22%) with a significant difference (OR= 2.56, 95%= 1.14-5.71, p= 0.022). the final conclusion carrying the mutant homozygous (CC) of the SNP rs8192917 and allele C of this SNP may have a higher chance of developing HCC compared with those carrying other genotypes and T allele of the SNP. The haplotype block CCG (corresponding for TC allele of rs7144366, C allele of rs8192917 and G allele of rs2236338) might be regarded as a risk factor for the emergence of HCC in patients with CHB.
{"title":"Granzyme B Gene Polymorphisms and Risk of Hepatocellular Carcinoma in Patients with Chronic Hepatitis","authors":"Hussein S. Alshamary, Q. Mayah, Fadhil Ridha","doi":"10.5455/jabet.2022.d137","DOIUrl":"https://doi.org/10.5455/jabet.2022.d137","url":null,"abstract":"Abstract Infection with the hepatitis B virus (HBV) continues to be a hazard for public health across the globe. Chronic hepatitis, cirrhosis, and hepatocellular carcinoma are all possible outcomes (HCC). It is obvious that certain patients with chronic hepatitis B (CHB) viral infection developed HCC, while other under almost similar circumstances do not. To inspect the possible there being a link between three single nucleotide gene polymorphisms (SNPs) in GzmB genes with the development of HCC. A total of 85 patients diagnosed with CHB participated in this research (40 patients with HCC and 45 patients without HCC). Three SNPs in GzmB gene (rs7144366, rs8192917 and rs2236338) were genotypes using restriction fragment length polymorphism (RFLP). The haplotype blocks derived from the three SNPs were assembled, and the linkage disequilibrium (LD) between the SNPs was determined using the SHEsis software. The homozygous mutant genotype (CC) was shown to be significantly more common in patients with HCC (27.5 %) than in those without HCC (11.11 %) (OR= 3.93, 95 percent CI=1.13-13.62, p=0.031). At allelic level, the mutant allele (C) was more frequent in patients with than those without HCC (46.25% vs. 26.67%) with a significant deviation (OR=2.36, 95%CI= 1.25- 4.49, p= 0.008). the haplotype block CCG was more common among patients with HCC (26.25%) than those without HCC (12.22%) with a significant difference (OR= 2.56, 95%= 1.14-5.71, p= 0.022). the final conclusion carrying the mutant homozygous (CC) of the SNP rs8192917 and allele C of this SNP may have a higher chance of developing HCC compared with those carrying other genotypes and T allele of the SNP. The haplotype block CCG (corresponding for TC allele of rs7144366, C allele of rs8192917 and G allele of rs2236338) might be regarded as a risk factor for the emergence of HCC in patients with CHB.","PeriodicalId":36275,"journal":{"name":"Journal of Advanced Biotechnology and Experimental Therapeutics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70796590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Melia azedarach is a plant of the Meliaceae family, used around the world in various medical fields. Because of the widespread incidence of thrombosis worldwide especially during the coronavirus epidemic, this study was conducted to evaluate the in vitro thrombolytic effect of methanolic extracts of Melia azedarach leaves and fruits. Series of dilutions starting from 2mg/ml to 20mg/ml were prepared from the methanolic extracts. The results showed that thrombolysis rates were between (18.7%-29.3%) for fruit extract in distilled water, ( 18.3%-30.1%) in phosphate buffer, (19.3%-35%) for leaves extract in distilled water and (20%-32.3%) in phosphate buffer for dilutions from 2 mg/ml to 20mg/ml. The thrombolytic effect for streptokinase (positive control) was 47.54% in distilled water, and 44.36% in phosphate buffer, compared with negative control which was 5.94% and 6.34% respectively. Phytochemical screening found flavonoids and coumarins in leaves extract and only flavonoids in fruit extract, so this may suggest that the increase in thrombolytic effect may be attributed to these flavonoids and coumarins. The total phenolic content was 15.78 (mg GAE1g) in leaves extract and 3.64 (mg GAE1g) in fruit extract, while the total flavonoid content was 0.813 (mg QE1g) in leaves extract, and 0.17 (mg QE1g) in fruit extract. In conclusion, these results showed that Melia azedarach has a thrombolytic effect.
{"title":"Evaluation of the thrombolytic activity of the methanolic extract of Melia azedarach fruits and leaves in vitro","authors":"H. Hussain, M. Agha","doi":"10.5455/jabet.2022.d142","DOIUrl":"https://doi.org/10.5455/jabet.2022.d142","url":null,"abstract":"Melia azedarach is a plant of the Meliaceae family, used around the world in various medical fields. Because of the widespread incidence of thrombosis worldwide especially during the coronavirus epidemic, this study was conducted to evaluate the in vitro thrombolytic effect of methanolic extracts of Melia azedarach leaves and fruits. Series of dilutions starting from 2mg/ml to 20mg/ml were prepared from the methanolic extracts. The results showed that thrombolysis rates were between (18.7%-29.3%) for fruit extract in distilled water, ( 18.3%-30.1%) in phosphate buffer, (19.3%-35%) for leaves extract in distilled water and (20%-32.3%) in phosphate buffer for dilutions from 2 mg/ml to 20mg/ml. The thrombolytic effect for streptokinase (positive control) was 47.54% in distilled water, and 44.36% in phosphate buffer, compared with negative control which was 5.94% and 6.34% respectively. Phytochemical screening found flavonoids and coumarins in leaves extract and only flavonoids in fruit extract, so this may suggest that the increase in thrombolytic effect may be attributed to these flavonoids and coumarins. The total phenolic content was 15.78 (mg GAE1g) in leaves extract and 3.64 (mg GAE1g) in fruit extract, while the total flavonoid content was 0.813 (mg QE1g) in leaves extract, and 0.17 (mg QE1g) in fruit extract. In conclusion, these results showed that Melia azedarach has a thrombolytic effect.","PeriodicalId":36275,"journal":{"name":"Journal of Advanced Biotechnology and Experimental Therapeutics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70796821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Huq, Muhammad Hossain, M. Islam, Marta Sobur, A. Rahman, M. Rahman
Coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a global pandemic of the century. The disease is wreaking havoc on human health, the world economy, society, and the environment. It has already caused the loss of millions of lives. Because of the mutation, the virus is constantly evolving itself, changing its nature including the disease transmission rate, virulence, pathogenesis, and clinical manifestations. It was recently reported that certain COVID-19 patients are also suffering from a fungal infection as co-infection commonly known as mucormycosis (black fungus). In India, the outbreak of black fungus in COVID-19 patients has already been declared an epidemic. Only a few reports are noticed in other countries. The focus must now be put toward better management and control of the COVID-19-associated fungal infection. In this review, we have discussed various aspects of black fungus particularly the etiology, taxonomy, risk factors, transmission, pathogenesis, clinical manifestations, diagnosis, and line of treatment to keep up to date on how to manage this fungal infection better.
{"title":"Mucormycosis (black fungus) and its impact on the COVID-19 patients: An updated review","authors":"A. Huq, Muhammad Hossain, M. Islam, Marta Sobur, A. Rahman, M. Rahman","doi":"10.5455/jabet.2022.d108","DOIUrl":"https://doi.org/10.5455/jabet.2022.d108","url":null,"abstract":"Coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a global pandemic of the century. The disease is wreaking havoc on human health, the world economy, society, and the environment. It has already caused the loss of millions of lives. Because of the mutation, the virus is constantly evolving itself, changing its nature including the disease transmission rate, virulence, pathogenesis, and clinical manifestations. It was recently reported that certain COVID-19 patients are also suffering from a fungal infection as co-infection commonly known as mucormycosis (black fungus). In India, the outbreak of black fungus in COVID-19 patients has already been declared an epidemic. Only a few reports are noticed in other countries. The focus must now be put toward better management and control of the COVID-19-associated fungal infection. In this review, we have discussed various aspects of black fungus particularly the etiology, taxonomy, risk factors, transmission, pathogenesis, clinical manifestations, diagnosis, and line of treatment to keep up to date on how to manage this fungal infection better.","PeriodicalId":36275,"journal":{"name":"Journal of Advanced Biotechnology and Experimental Therapeutics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70796130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Chemical characterization, antimicrobial, antioxidant, and larvicidal activities of certain fungal extracts","authors":"Asmaa Motleb, M. Aziz, M. Ghareeb, Maha Shazly","doi":"10.5455/jabet.2022.d128","DOIUrl":"https://doi.org/10.5455/jabet.2022.d128","url":null,"abstract":"","PeriodicalId":36275,"journal":{"name":"Journal of Advanced Biotechnology and Experimental Therapeutics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70796484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The aim of this study was to compare the level of SHBG (globulin binding hormone sex) in diabetic pregnant women compared with healthy pregnant women in Kerman.This descriptive cross-sectional study was performed on diabetic pregnant patients whose patients were confirmed by a 2-hour blood glucose tolerance test using 50 g glucose or three hours with 100 g glucose. The clinic of Afzalipour Hospital in Kerman was conducted in 1397-8. After completion of demographic information and midwifery records blood samples were collected from the patients and the blood glucose levels were measured, SHBG was measured and finally all data were collected and analyzed for soft The SPSS software was analyzed and analyzed. The serum levels of SHBG in the case group were 83.5 ± 66.4 and 142.9 ± 65 in the control group, which is statistically different (P value = 0.000(. The data from this study showed that in patients with gestational diabetes, serum levels of SHBG significantly decreased, but this level reduction was not significantly associated with other underlying factors and midwifery factors.
{"title":"Plasma sex hormone-binding globulin (SHBG) level: A comparative study between patients with gestational diabetes mellitus and healthy pregnant women","authors":"Abnoos Mokhtari, Roza Hasanizadeh","doi":"10.5455/jabet.2022.d150","DOIUrl":"https://doi.org/10.5455/jabet.2022.d150","url":null,"abstract":"The aim of this study was to compare the level of SHBG (globulin binding hormone sex) in diabetic pregnant women compared with healthy pregnant women in Kerman.This descriptive cross-sectional study was performed on diabetic pregnant patients whose patients were confirmed by a 2-hour blood glucose tolerance test using 50 g glucose or three hours with 100 g glucose. The clinic of Afzalipour Hospital in Kerman was conducted in 1397-8. After completion of demographic information and midwifery records blood samples were collected from the patients and the blood glucose levels were measured, SHBG was measured and finally all data were collected and analyzed for soft The SPSS software was analyzed and analyzed. The serum levels of SHBG in the case group were 83.5 ± 66.4 and 142.9 ± 65 in the control group, which is statistically different (P value = 0.000(. The data from this study showed that in patients with gestational diabetes, serum levels of SHBG significantly decreased, but this level reduction was not significantly associated with other underlying factors and midwifery factors.","PeriodicalId":36275,"journal":{"name":"Journal of Advanced Biotechnology and Experimental Therapeutics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70796503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Polycystic ovary syndrome (PCOS) is a heterogeneous genetic disorder categorized by hyperandrogenism that affects early reproductive age in females, KISS1 has play role in regulating the hypothalamic-pituitary-gonad axis, it plays a key role in human reproductive function. In patients with polycystic ovarian syndrome, imbalance-of-function mutations in this gene have been found often. blood samples were collected from 120 patients (60 control are divided into 30 normal weight and 30 obese) and (60 PCOS) females are divided into 30 normal weight and 30 obese). DNA was extracted and genotyped for KISS1 variants by HRM-PCR and measured level of kisspeptin by ELIAS, while LH, FSH, DHEA and free testosterone by CLIA. The value of LH, Testosterone, DHEA-S and kisspeptin is elevated in the patient group, while the decline of FSH in serum level patients value, rs372790354 G > A and rs4889 G>A was associated with PCOS in dominant, recessive, co-dominant (P-value< 0.05), rs37279054 AA was not found the effect of obese group and linked with normal weight PCOS put present study no effect on the parameters, rs4889 GG/GA was the effect on all subgroups except the genotype GA not effected on obese female, the highly significant ( P-value<0.05) of rs4889 GA influenced on measured of WHR, LH/FSH ratio and DHEA-S in the patient compared to control, rs4889 GG/AA was influenced on normal-weight patient compare to an obese patient, the WHR was higher in an obese patient in both genotype. While the level of kisspeptin in normal weight with genotype AA was higher level compared to obese and (P-value<0.05). We concluded that the KISS1 levels were higher in PCOS females compared to controls and decreased with increasing BMI, Kiss1 polymorphism rs372790354 G>A and rs4889 G>A may be associated with the pathophysiology of PCOS and lead to increase serum level of LH that due to hyperandrogenism.
多囊卵巢综合征(Polycystic ovarian syndrome, PCOS)是一种影响女性早育年龄的异源性遗传疾病，KISS1在调节下丘脑-垂体-性腺轴中发挥重要作用，在人类生殖功能中起关键作用。在多囊卵巢综合征患者中，经常发现该基因的功能不平衡突变。采集120例患者(对照组60例，体重正常30例，肥胖30例)和PCOS女性60例，体重正常30例，肥胖30例)的血液样本。提取DNA，采用HRM-PCR分型，elisa检测kisspeptin水平，CLIA检测LH、FSH、DHEA和游离睾酮水平。LH的价值,睾酮、硫酸脱氢表雄酮(dhea - s和kisspeptin升高的患者群体,而病人血清中FSH水平的下降值,rs372790354 G >和rs4889 G >主导与PCOS有关,隐性,共显性(p < 0.05),未找到rs37279054 AA的影响肥胖组和正常体重与PCOS把本研究没有对参数的影响,rs4889 GG / GA对所有子组的影响除了基因型遗传算法不影响肥胖的女性,高p值ea和rs4889 G>A可能与PCOS的病理生理有关，并导致高雄激素血症引起的血清LH水平升高。
{"title":"Effect of KISS1 gene variants (rs372790354 G>A and rs4889 G>A) on kisspeptin in patients with polycystic ovary syndrome in Iraq.","authors":"Nora Musawi, S. Qaysi, S. Witwit","doi":"10.5455/jabet.2022.d136","DOIUrl":"https://doi.org/10.5455/jabet.2022.d136","url":null,"abstract":"Polycystic ovary syndrome (PCOS) is a heterogeneous genetic disorder categorized by hyperandrogenism that affects early reproductive age in females, KISS1 has play role in regulating the hypothalamic-pituitary-gonad axis, it plays a key role in human reproductive function. In patients with polycystic ovarian syndrome, imbalance-of-function mutations in this gene have been found often. blood samples were collected from 120 patients (60 control are divided into 30 normal weight and 30 obese) and (60 PCOS) females are divided into 30 normal weight and 30 obese). DNA was extracted and genotyped for KISS1 variants by HRM-PCR and measured level of kisspeptin by ELIAS, while LH, FSH, DHEA and free testosterone by CLIA. The value of LH, Testosterone, DHEA-S and kisspeptin is elevated in the patient group, while the decline of FSH in serum level patients value, rs372790354 G > A and rs4889 G>A was associated with PCOS in dominant, recessive, co-dominant (P-value< 0.05), rs37279054 AA was not found the effect of obese group and linked with normal weight PCOS put present study no effect on the parameters, rs4889 GG/GA was the effect on all subgroups except the genotype GA not effected on obese female, the highly significant ( P-value<0.05) of rs4889 GA influenced on measured of WHR, LH/FSH ratio and DHEA-S in the patient compared to control, rs4889 GG/AA was influenced on normal-weight patient compare to an obese patient, the WHR was higher in an obese patient in both genotype. While the level of kisspeptin in normal weight with genotype AA was higher level compared to obese and (P-value<0.05). We concluded that the KISS1 levels were higher in PCOS females compared to controls and decreased with increasing BMI, Kiss1 polymorphism rs372790354 G>A and rs4889 G>A may be associated with the pathophysiology of PCOS and lead to increase serum level of LH that due to hyperandrogenism.","PeriodicalId":36275,"journal":{"name":"Journal of Advanced Biotechnology and Experimental Therapeutics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70796583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Moniruzzaman, M. Jinnah, Most Mostari, Shirmin Islam, M. Pramanik, Jui Biswas, Alam Imran, M. Uddin, M. Saleh, S. Zaman
Pesticides are necessary in agriculture, yet their highly toxic ingredients harm the ecosystem. Due of their toxicity, uncontrolled releases of large quantities of pesticides pollute the environment and provide a larger health risk to plants, animals, and humans. Bacteria are capable of degrading such pollutants and saving our ecosystem. In this work, a bacterial strain was isolated from Shobicron and Vertimec-treated lady's finger soil using enrichment culture. The strain was identified as Burkholderia Cepacia-Like MB-01 based on morphological, physiological, and biochemical traits, as well as phylogenetic analysis of the 16SrRNA sequence. The bacterium grew best at 35 °C with a pH of 7. Furthermore, it was susceptible (S) to Cefepime and Penicillin but intermediate (I) resistant to Carbapenem and Tetracycline and resistant (R) to Ciprofloxacin, Kanamycin, and Gentamycin in an antibiotic sensitivity test. The rate of shobicron and vertimec degradation was measured over a five-day period using Mineral Salt (MS) medium. In its optimum growth condition, shobicron and vertimec degradation rates were determined to be around 76 % and 80 %, respectively. The isolated bacterial strain was found to be capable of detoxifying shobicron and vertimec in the experiment. As a result, the bacterial strain could be exploited as a possible shobicron and vertimec degrader for pesticide bioremediation.
{"title":"Efficacy of soil-borne Burkholderia Cepacia-Like MB-01 strain for shobicron and vertimec degradation","authors":"M. Moniruzzaman, M. Jinnah, Most Mostari, Shirmin Islam, M. Pramanik, Jui Biswas, Alam Imran, M. Uddin, M. Saleh, S. Zaman","doi":"10.5455/jabet.2022.d143","DOIUrl":"https://doi.org/10.5455/jabet.2022.d143","url":null,"abstract":"Pesticides are necessary in agriculture, yet their highly toxic ingredients harm the ecosystem. Due of their toxicity, uncontrolled releases of large quantities of pesticides pollute the environment and provide a larger health risk to plants, animals, and humans. Bacteria are capable of degrading such pollutants and saving our ecosystem. In this work, a bacterial strain was isolated from Shobicron and Vertimec-treated lady's finger soil using enrichment culture. The strain was identified as Burkholderia Cepacia-Like MB-01 based on morphological, physiological, and biochemical traits, as well as phylogenetic analysis of the 16SrRNA sequence. The bacterium grew best at 35 °C with a pH of 7. Furthermore, it was susceptible (S) to Cefepime and Penicillin but intermediate (I) resistant to Carbapenem and Tetracycline and resistant (R) to Ciprofloxacin, Kanamycin, and Gentamycin in an antibiotic sensitivity test. The rate of shobicron and vertimec degradation was measured over a five-day period using Mineral Salt (MS) medium. In its optimum growth condition, shobicron and vertimec degradation rates were determined to be around 76 % and 80 %, respectively. The isolated bacterial strain was found to be capable of detoxifying shobicron and vertimec in the experiment. As a result, the bacterial strain could be exploited as a possible shobicron and vertimec degrader for pesticide bioremediation.","PeriodicalId":36275,"journal":{"name":"Journal of Advanced Biotechnology and Experimental Therapeutics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70796689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study aimed to analyze the distribution of phytoplankton abundance and its relationship with water quality in Lasolo Bay waters. The study was conducted during the West Season 2020. The sample consisted of 9 stations located near the mouth of the Lasolo River (station A), coastal waters (Station B) and the center part of Lasolo Bay (Station C). Phytoplankton sampling was using plankton nets with 25 µm meshsize. Identification of phytoplankton used microscope and the abundance calculation by using Sedgwick Rafter Counting Cell. The results showed that there are 23 phytoplankton genera from 5 classes found, namely 12 genera of Bacillariophycea Class, 5 genera of Dinophyceae Class, 3 genera of Chlorophyceae Class, 2 genera of Cyanophyceae class and 1 genera of Coscinodiscophyceae class. By abundance, phytoplankton compositions of the class Bacillariophyceae predominate with a percentage of 57%. The type of phytoplankton that has the highest abundance is Nitzschia sp. of 2,407 cells/l and the lowest abundance of Amphidium sp. of 1,368 cells/l. Phytoplankton abundance at Station A is 41,093 cells/l, station B is 14,234 cells/l and station C is 14,735 cells/l. The distribution of phytoplankton abundance is also influenced by physical and chemical factors such as turbidity, TSS, Nitrate and Phosphate.
{"title":"Spatial distribution of phytoplankton in Lasolo Bay of Southeast Sulawesi Province, Indonesia","authors":"Ira Irawati, L. Sara, M. Muliddin, A. Asriyana","doi":"10.5455/jabet.2022.d140","DOIUrl":"https://doi.org/10.5455/jabet.2022.d140","url":null,"abstract":"This study aimed to analyze the distribution of phytoplankton abundance and its relationship with water quality in Lasolo Bay waters. The study was conducted during the West Season 2020. The sample consisted of 9 stations located near the mouth of the Lasolo River (station A), coastal waters (Station B) and the center part of Lasolo Bay (Station C). Phytoplankton sampling was using plankton nets with 25 µm meshsize. Identification of phytoplankton used microscope and the abundance calculation by using Sedgwick Rafter Counting Cell. The results showed that there are 23 phytoplankton genera from 5 classes found, namely 12 genera of Bacillariophycea Class, 5 genera of Dinophyceae Class, 3 genera of Chlorophyceae Class, 2 genera of Cyanophyceae class and 1 genera of Coscinodiscophyceae class. By abundance, phytoplankton compositions of the class Bacillariophyceae predominate with a percentage of 57%. The type of phytoplankton that has the highest abundance is Nitzschia sp. of 2,407 cells/l and the lowest abundance of Amphidium sp. of 1,368 cells/l. Phytoplankton abundance at Station A is 41,093 cells/l, station B is 14,234 cells/l and station C is 14,735 cells/l. The distribution of phytoplankton abundance is also influenced by physical and chemical factors such as turbidity, TSS, Nitrate and Phosphate.","PeriodicalId":36275,"journal":{"name":"Journal of Advanced Biotechnology and Experimental Therapeutics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70796794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N. Poly, Sabrina Mamtaz, Mohammad Khan, M. Hoque, A. Azad, M. Hasan
{"title":"Isolation, documentation and biochemical characterization of cellulolytic bacteria from rumen fluid of cattle","authors":"N. Poly, Sabrina Mamtaz, Mohammad Khan, M. Hoque, A. Azad, M. Hasan","doi":"10.5455/jabet.2022.d126","DOIUrl":"https://doi.org/10.5455/jabet.2022.d126","url":null,"abstract":"","PeriodicalId":36275,"journal":{"name":"Journal of Advanced Biotechnology and Experimental Therapeutics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70796084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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