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Food insecurity and the dental team: a pilot study to explore opinions. 粮食不安全与牙科团队:一项探索意见的试点研究。
IF 3 Q2 Dentistry Pub Date : 2024-03-18 DOI: 10.1038/s41405-024-00205-8
Sondos Albadri, Lisa Allen, Teslimat Ajeigbe

Purpose: In total, 17% of UK households with children experience food insecurity, with evidence to suggest a direct correlation with the prevalence of oral disease. This study explores current perceptions of the dental team, when recognising and supporting families who may experience food insecurity.

Materials and methods: An online, anonymous cross-sectional survey was designed and sent to members of the British Society of Paediatric Dentistry (BSPD) in June 2023, examining confidence and understanding surrounding food insecurity and dental health. Quantitative data is presented descriptively and qualitative data using a thematic analysis.

Results: The response rate was 9.6% (n = 76). A significant number recognise the link between poor oral health and food insecurity, 80.3% (n = 61). Although practitioners are confident in oral health counselling, 80.3% (n = 61) a smaller proportion are not as confident when approaching food insecurity 32.9% (n = 25). Dental team members recognise the need to improve identification of affected patients and that they have a professional duty to support. Intervention strategies, such as additional training to support team development and signposting of patients are indicated.

Conclusion: This study suggests that whilst dental professionals understand the link between food insecurity and oral health, and their responsibilities to those affected; they lack confidence in identifying such patients and providing support. Additional conversations and training are fundamental to better understand their role, which must reflect the needs of the population that they serve.

目的:在英国有儿童的家庭中,总共有 17% 的家庭经历过粮食不安全,有证据表明这与口腔疾病的发病率直接相关。本研究探讨了牙科团队在识别和支持可能出现食物无保障的家庭时的现有看法:我们设计了一项在线匿名横断面调查,并于 2023 年 6 月发送给英国儿童牙科协会 (BSPD) 的成员,调查他们对食物不安全和牙齿健康的信心和理解。定量数据以描述性方式呈现,定性数据则采用主题分析法:答复率为 9.6%(n = 76)。相当多的人认识到口腔健康不良与粮食不安全之间的联系,占 80.3%(n = 61)。虽然从业人员对口腔健康咨询很有信心,占 80.3%(n = 61),但也有一小部分人在处理食物不安全问题时信心不足,占 32.9%(n = 25)。牙科团队成员认识到有必要加强对受影响患者的识别,并认识到他们有提供支持的专业责任。研究指出了一些干预策略,如提供额外培训以支持团队发展和为患者提供转介服务:这项研究表明,虽然牙科专业人员了解食物不安全与口腔健康之间的联系,以及他们对受影响者的责任,但他们在识别这类患者和提供支持方面缺乏信心。额外的对话和培训对于更好地理解他们的角色至关重要,他们的角色必须反映出他们所服务人群的需求。
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引用次数: 0
Can diode laser 810 nm decrease post endodontic pain in patients with asymptomatic necrotic maxillary incisors? A four-arm randomized controlled trial. 波长为 810 nm 的二极管激光能否减轻无症状坏死上颌切牙患者牙髓治疗后的疼痛?一项四臂随机对照试验。
IF 3 Q2 Dentistry Pub Date : 2024-03-14 DOI: 10.1038/s41405-024-00203-w
Mohammad Tamer Abbara, Samar Akil, Omar Hamadah, Hassan Achour, Ghina Mahayni, Yasser Alsayed Tolibah

Aim: To find the best method for applying the diode laser 810 nm to relieve post-endodontic pain on necrotic maxillary incisors with periapical lesions within a single-visit treatment.

Methods: Eighty patients with a necrotic incisor, diagnosed with asymptomatic apical periodontitis, received standardized cleaning and shaping procedures, then divided randomly with a 1:1:1:1 allocation ratio into four groups: Group 1: control group with no laser application, Group 2: applying the diode laser as an irrigation activation system (IAS), Group 3: applying the diode laser from the buccal and palatal mucosa, Group 4: applying the diode laser as an IAS and from buccal and palatal mucosa. The postoperative pain was assessed using the visual analog scale (VAS) 1, 3, 7, and 14 days after the treatment. The mean values of the VAS score were statistically analyzed used Kruskal-Walis and Mann-Whitney U tests. The level of significance was set at a = 0.05.

Results: During 14 days after treatment, there was a statistically significant difference between mean values of VAS scores in the four groups (P value < 0.05); Group 1 scored the highest score, whereas Group 4 showed the lowest one. Moreover, Group 4 showed favorable outcomes compared with Group 2 and Group 3 during the first three days after treatment.

Conclusion: Diode laser reduced postoperative pain after necrotic teeth with large-sized apical lesion treatment, whereas using diode laser either as an IAS or LLLT reduced the postoperative pain compared with the control group. Moreover, the usage of a diode laser in both previous techniques represents the best protocol for postoperative pain relief during 14 days of treatment.

Clinical relevance: The clinical significance of this study is to investigate the best method to reduce postoperative pain using diode lasers 810 nm; where the results of this study indicated that the more diode laser exposer in LLLT and IAS, the less postoperative pain after endodontic procedures.

目的:在一次就诊治疗中,找到应用 810 纳米二极管激光缓解上颌切牙根尖周炎坏死后疼痛的最佳方法:80名被诊断为无症状根尖牙周炎的坏死切牙患者接受标准化的清洁和塑形程序,然后按1:1:1:1的分配比例随机分为四组:第一组:不使用激光的对照组;第二组:使用二极管激光作为灌洗激活系统(IAS);第三组:从颊腭粘膜使用二极管激光;第四组:使用二极管激光作为灌洗激活系统并从颊腭粘膜使用。治疗后 1、3、7 和 14 天,使用视觉模拟量表(VAS)对术后疼痛进行评估。VAS 评分的平均值采用 Kruskal-Walis 和 Mann-Whitney U 检验进行统计分析。显著性水平设定为 a = 0.05:结果:在治疗后的 14 天内,四组 VAS 评分的平均值之间存在显著的统计学差异(P 值):与对照组相比,使用二极管激光作为 IAS 或 LLLT 可减少坏死牙和大面积根尖病变治疗后的术后疼痛。此外,在之前的两种技术中,二极管激光的使用代表了在 14 天治疗期间缓解术后疼痛的最佳方案:本研究的临床意义在于探讨使用 810 纳米二极管激光减轻术后疼痛的最佳方法;本研究的结果表明,LLLT 和 IAS 中的二极管激光照射量越大,牙髓治疗术后的疼痛就越轻。
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引用次数: 0
Moringa oleifera leaf extract promotes the healing of critical sized bone defects in the mandibles of rabbits. 油辣木叶提取物可促进兔子下颌骨临界大小骨缺损的愈合。
IF 3 Q2 Dentistry Pub Date : 2024-03-14 DOI: 10.1038/s41405-024-00201-y
Nouran A Elsadek, Maha A Aboukhadr, Fatma R Kamel, Hossam M Mostafa, Gillan I El-Kimary

Objective: The search for an osteopromotive material that enhances the efficacy of alloplasts in reconstructive surgeries has been going on for years. This study aimed to histologically and histomorphometrically evaluate the efficiency of Moringa oleifera leaf extract as an osteopromotive biomaterial.

Design: The study is a prospective randomized controlled animal study. 24 adult male New Zealand rabbits were equally allocated into test and control groups. Critical-sized bone defects were created in the edentulous areas of the mandibles of rabbits. The defects of the control group were filled with Beta-tricalcium Phosphate, while the defects of the test group were filled with Beta-tricalcium Phosphate combined with Moringa oleifera leaf extract. The results were evaluated histologically and histomorphometrically.

Results: Histological and histomorphometric analysis showed a significant increase in the surface area of bone and the number of osteoblasts in test groups compared to those in the control groups.

Conclusion: Moringa oleifera leaf extract has a positive effect on bone regeneration in critical-sized bone defects.

目的:多年来,人们一直在寻找一种骨动力材料,以增强异体材料在整形手术中的功效。本研究旨在从组织学和组织形态计量学角度评估辣木叶提取物作为骨促进生物材料的功效:本研究是一项前瞻性随机对照动物研究。将 24 只成年雄性新西兰兔平均分为试验组和对照组。试验组在兔子下颌无牙区创建临界大小的骨缺损。对照组的缺损用 Beta 磷酸三钙填充,而试验组的缺损用 Beta 磷酸三钙和辣木叶提取物混合填充。对结果进行了组织学和组织形态计量学评估:结果:组织学和组织形态计量学分析表明,与对照组相比,试验组的骨表面积和成骨细胞数量显著增加:结论:油辣木叶提取物对临界大小骨缺损的骨再生有积极作用。
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引用次数: 0
Combination of stem cell-derived secretome from human exfoliated deciduous teeth with Yemeni Sidr honey on cell viability and migration: an in vitro study. 从人类脱落牙齿中提取的干细胞分泌物与也门 Sidr 蜂蜜相结合对细胞活力和迁移的影响:体外研究。
IF 2.5 Q2 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2024-03-13 DOI: 10.1038/s41405-024-00197-5
Mona Abdulrahman Abdullah Al-Hadi

Introduction: Bone diseases have a profound global impact, especially when the body's innate regenerative capacity falls short in the face of extensive damage. Stem cells from human exfoliated deciduous teeth (SHEDs), discovered in 2003, offer a promising solution for tissue repair, as they self-renew naturally and are easily obtainable. Mesenchymal stem cells (MSCs), including SHEDs, are believed to promote tissue regeneration by releasing growth factors, collectively known as the secretome.

Aims: This study explored the potential of combining SHED-derived secretome with Yemeni Sidr honey to improve osteoblast and fibroblast cell viability and migration.

Materials and methods: The experiment involved treating cell cultures of two types of rat cell lines - 7F2 osteoblast and BHK-21 fibroblast immortalized cells - with SHED-derived secretome and Yemeni Sidr honey. After the treatment, cell viability was measured using the MTT assay, which calculates OD at 590 nm. Additionally, the scratch assay was conducted to evaluate cell migration, and ImageJ software was used for data processing.

Results: The findings indicated that combining SHED-derived secretome and Yemeni Sidr honey enhanced osteoblast and fibroblast cell viability and migration. Furthermore, the study highlighted the difference in the stimulative potential of SHED-derived secretome, Yemeni Sidr honey, and their combination, on the viability and migration of the cultured cells.

Conclusion: The research concludes that combining SHED-derived secretome with Yemeni Sidr honey has the potential to promote cell viability and migration in in-vitro settings. The synergistic application of these substances has been found to be more effective -when combined in a dose-dependent manner- than their counterparts. Overall, the current study serves as a foundation for further investigations to establish if the explored substance has any useful clinical applications.

导言:骨骼疾病对全球影响深远,尤其是当人体的先天再生能力不足以应对大面积损伤时。2003年发现的人类脱落牙齿(SHEDs)中的干细胞为组织修复提供了一种很有前景的解决方案,因为它们可以自然自我更新,而且很容易获得。研究目的:本研究探讨了将SHED提取的分泌物与也门Sidr蜂蜜相结合,以提高成骨细胞和成纤维细胞活力和迁移的潜力:实验包括用 SHED 衍生的分泌物和也门 Sidr 蜂蜜处理两种类型的大鼠细胞系--7F2 成骨细胞和 BHK-21 成纤维细胞。处理后,使用 MTT 检测法测量细胞存活率,该方法在 590 纳米波长下计算 OD 值。此外,还进行了划痕试验以评估细胞迁移,并使用 ImageJ 软件进行数据处理:结果:研究结果表明,将 SHED 衍生的分泌物与也门 Sidr 蜂蜜结合使用可提高成骨细胞和成纤维细胞的活力和迁移能力。此外,研究还强调了 SHED 衍生的分泌物、也门 Sidr 蜂蜜和它们的组合对培养细胞的活力和迁移的刺激潜力的差异:研究得出结论,将 SHED 提取的分泌物与也门西德蜂蜜结合使用,有可能促进体外培养细胞的活力和迁移。研究发现,当这些物质以剂量依赖的方式结合在一起时,其协同作用比其对应物质更有效。总之,目前的研究为进一步研究奠定了基础,以确定所探索的物质是否具有任何有用的临床应用价值。
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引用次数: 0
Efficacy of BioMin F and NovaMin toothpastes against streptococcus mutans: an in vitro study. BioMin F 和 NovaMin 牙膏对变异链球菌的功效:一项体外研究。
IF 3 Q2 Dentistry Pub Date : 2024-03-11 DOI: 10.1038/s41405-024-00202-x
Haya Doura Alomari, Yasser Alsayed Tolibah, Chaza Kouchaji

Objective: This in vitro study was accomplished to demonstrate the antibacterial efficacy of BioMin F and NovaMin toothpastes against the recently-isolated Streptococcus Mutans in comparison with a commonly used fluoride toothpaste.

Materials and methods: Dental plaque collection method was adopted to isolate streptococcus mutans in children with dental caries. Then an ideal Streptococcus Mutans colony was incubated in 20 Petri dishes that contained Mueller-Hinton medium. Each dish had 3 wells; one well for each toothpaste (BioMin F, NovMin, and Signal) to perform the agar diffusion test. After incubating for 24 hours, the inhabitation zone around each well of each Petri dish was noticed and measured. Statistical Analysis was achieved using a statistical package, SPSS Windows version 17, by applying Kruskal-Wallis with Mann-Whitney U test (α = 0.05).

Results: BioMin F showed the highest mean of inhibition zone diameter ( x ¯  = 2.67 mm) in compared with NovaMin and Signal ( x ¯  = 0.39 mm and x ¯  = 2.19 mm; p < 0.001 in each pairwise comparison).

Conclusion: BioMin F toothpaste showed superior antibacterial effect against Streptococcus mutans to Signal and NovaMin toothpastes. Novamin showed the lowest antibacterial effect. This in vitro study suggests that BioMin F toothpaste shows encouraging potential to be recommended as a preventive measure to reduce the caries risk.

研究目的本体外研究旨在证明 BioMin F 和 NovaMin 牙膏与常用含氟牙膏相比,对最近分离出的变异链球菌的抗菌效果:采用牙菌斑采集法分离龋齿儿童中的变异链球菌。然后将理想的变异链球菌菌落培养在装有穆勒-欣顿培养基的 20 个培养皿中。每个培养皿有 3 个孔;每种牙膏(BioMin F、NovMin 和 Signal)一个孔,以进行琼脂扩散试验。培养 24 小时后,观察并测量每个培养皿中每个孔周围的栖息区。使用 SPSS Windows 17 版统计软件包进行统计分析,采用 Kruskal-Wallis 和 Mann-Whitney U 检验(α = 0.05):与 NovaMin 和 Signal(x¯ = 0.39 mm 和 x¯ = 2.19 mm;p)相比,BioMin F 的抑菌区直径(x¯ = 2.67 mm)平均值最高:BioMin F 牙膏对变异链球菌的抗菌效果优于 Signal 和 NovaMin 牙膏。Novamin 的抗菌效果最低。这项体外研究表明,BioMin F 牙膏具有令人鼓舞的潜力,可推荐用作降低龋齿风险的预防措施。
{"title":"Efficacy of BioMin F and NovaMin toothpastes against streptococcus mutans: an in vitro study.","authors":"Haya Doura Alomari, Yasser Alsayed Tolibah, Chaza Kouchaji","doi":"10.1038/s41405-024-00202-x","DOIUrl":"10.1038/s41405-024-00202-x","url":null,"abstract":"<p><strong>Objective: </strong>This in vitro study was accomplished to demonstrate the antibacterial efficacy of BioMin F and NovaMin toothpastes against the recently-isolated Streptococcus Mutans in comparison with a commonly used fluoride toothpaste.</p><p><strong>Materials and methods: </strong>Dental plaque collection method was adopted to isolate streptococcus mutans in children with dental caries. Then an ideal Streptococcus Mutans colony was incubated in 20 Petri dishes that contained Mueller-Hinton medium. Each dish had 3 wells; one well for each toothpaste (BioMin F, NovMin, and Signal) to perform the agar diffusion test. After incubating for 24 hours, the inhabitation zone around each well of each Petri dish was noticed and measured. Statistical Analysis was achieved using a statistical package, SPSS Windows version 17, by applying Kruskal-Wallis with Mann-Whitney U test (α = 0.05).</p><p><strong>Results: </strong>BioMin F showed the highest mean of inhibition zone diameter ( <math> <mover><mrow><mi>x</mi></mrow> <mo>¯</mo></mover> </math>  = 2.67 mm) in compared with NovaMin and Signal ( <math> <mover><mrow><mi>x</mi></mrow> <mo>¯</mo></mover> </math>  = 0.39 mm and <math> <mover><mrow><mi>x</mi></mrow> <mo>¯</mo></mover> </math>  = 2.19 mm; p < 0.001 in each pairwise comparison).</p><p><strong>Conclusion: </strong>BioMin F toothpaste showed superior antibacterial effect against Streptococcus mutans to Signal and NovaMin toothpastes. Novamin showed the lowest antibacterial effect. This in vitro study suggests that BioMin F toothpaste shows encouraging potential to be recommended as a preventive measure to reduce the caries risk.</p>","PeriodicalId":36997,"journal":{"name":"BDJ Open","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10928140/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140102551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Exploring collaboration reasons and leadership styles in Dutch primary oral healthcare practices. 探索荷兰初级口腔医疗实践中的合作原因和领导风格。
IF 3 Q2 Dentistry Pub Date : 2024-03-08 DOI: 10.1038/s41405-024-00200-z
Joost C L den Boer, Wil J M van der Sanden, Katarina Jerković-Ćosić, Josef J M Bruers

Aims: To outline the extent to which practice owners in Dutch oral healthcare practices (OHPs) use a directive and supportive leadership styles, to map out which goals practice owners in Dutch OHPs consider most important when choosing collaboration within the practice and to identify the reasons why oral healthcare professionals choose to engage in collaborative practice.

Materials and methods: A survey involving 802 general dental practitioners, dental hygienists, and prevention assistants was conducted. The questionnaire covered, among other subjects, leadership styles and reasons for collaboration. Data analysis included descriptive statistics, chi-square tests, one-way ANOVA, linear regression, and logistic regression.

Results: Compared to employees, practice owners ascribe to themselves more characteristics of both directive and supportive leadership. The most frequently mentioned reasons for choosing a practice form that involves collaboration were the possibilities to provide the best care and the desire to focus on prevention. Healthcare providers chose to work in a collaborative practice for several reasons, which were associated with profession, age and gender.

Conclusions: The degree of directive and supportive leadership among practice owners in dental care practices in the Netherlands showed a strong correlation. The most frequently mentioned reasons for choosing collaboration were related to healthcare content.

目的:概述荷兰口腔医疗机构(OHPs)的执业者在多大程度上使用指令性和支持性领导风格,描绘荷兰口腔医疗机构的执业者在选择执业合作时认为哪些目标最重要,并确定口腔医疗专业人员选择参与合作执业的原因:对 802 名全科牙科医生、牙科保健师和预防助理进行了调查。问卷内容包括领导风格和合作原因等。数据分析包括描述性统计、卡方检验、单因素方差分析、线性回归和逻辑回归:结果:与员工相比,诊所所有者认为自己更具有指令型和支持型领导的特点。选择涉及合作的执业形式,最常提及的原因是提供最佳医疗服务的可能性和注重预防的愿望。医疗服务提供者选择在合作实践中工作有几个原因,这些原因与职业、年龄和性别有关:结论:在荷兰,牙科医疗机构所有人的指导性领导和支持性领导的程度有很大的相关性。最常提及的选择合作的原因与医疗保健内容有关。
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引用次数: 0
Impact of dental visiting patterns on oral health: A systematic review of longitudinal studies. 看牙模式对口腔健康的影响:纵向研究的系统回顾。
IF 2.5 Q2 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2024-03-06 DOI: 10.1038/s41405-024-00195-7
Aina Najwa Mohd Khairuddin, Birke Bogale, Jing Kang, Jennifer E Gallagher

Aim: To systematically review longitudinal studies investigating the impact of dental visiting patterns on oral health across the life course.

Methods: Five databases (MEDLINE, Embase, Scopus, Web of Science, CINAHL) were searched up to March 2023. Results were screened based on eligibility criteria in a two-stage process: title and abstract, and full-text review. A backward search of reference lists and a forward search of citations of the included papers was also conducted. The quality of the included papers was assessed using the Newcastle-Ottawa Scale. Key study information was extracted and a narrative synthesis of the findings was performed.

Results: Eleven papers from five longitudinal studies in five countries (Australia, Brazil, China, New Zealand, Sweden) met the inclusion criteria. Studies of moderate to high quality consistently reported that regular dental attendance was associated with having less dental caries experience, fewer missing teeth and better oral health-related quality of life. Inconsistent findings were observed for decayed teeth, and no association was found for periodontal condition.

Conclusions: This review highlights an association between regular dental visiting pattern and improved oral health, notably less dental caries experience and better oral health-related quality of life. Dental attendance emerges as an important predictor of oral health across the life course, underscoring the importance of routine dental care.

Registration information: The PROSPERO registration number is CRD42023396380.

目的:系统回顾调查看牙模式对整个生命过程中口腔健康影响的纵向研究:检索了截至 2023 年 3 月的五个数据库(MEDLINE、Embase、Scopus、Web of Science、CINAHL)。根据资格标准分两个阶段对结果进行筛选:标题和摘要以及全文审阅。此外,还对纳入论文的参考文献目录进行了后向检索,并对引用文献进行了前向检索。采用纽卡斯尔-渥太华量表对纳入论文的质量进行评估。提取了关键研究信息,并对研究结果进行了叙述性综述:来自五个国家(澳大利亚、巴西、中国、新西兰和瑞典)五项纵向研究的 11 篇论文符合纳入标准。中等至高质量的研究一致报告称,定期看牙医与龋齿发生率较低、缺失牙齿较少以及口腔健康相关生活质量较高有关。关于蛀牙的研究结果不一致,而关于牙周状况的研究则没有发现相关性:本综述强调了定期看牙与改善口腔健康之间的关系,特别是龋齿经历较少和口腔健康相关生活质量较高。牙科就诊是整个生命过程中口腔健康的重要预测因素,强调了常规牙科保健的重要性:PROSPERO 注册号为 CRD42023396380。
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引用次数: 0
Lin28 affects the proliferation and osteogenic differentiation of human dental pulp stem cells by directly inhibiting let-7b maturation Lin28通过直接抑制let-7b的成熟影响人牙髓干细胞的增殖和成骨分化
IF 3 Q2 Dentistry Pub Date : 2024-03-05 DOI: 10.1038/s41405-024-00194-8
Liu Yan, Jing Sun, Yushan Wang, Xinxin Liu, Jiayi Hu, Mengxin Sun, Xi Suo, Rongquan Duan, Changyong Yuan

Objective

Activation of Lin28 gene under certain conditions promotes tissue damage repair. However, it remains unknown whether conditional expression of Lin28 facilitates the recovery of damaged pulp tissue. In the study, we focus on exploring the effects and possible regulatory mechanisms of Lin28 on the proliferation and differentiation of human dental pulp stem cells (hDPSCs).

Materials and methods

We adopted techniques such as the ethynyl-2ʹ-deoxyuridine (EdU) incorporation assay, RNA-protein immunoprecipitation (RIP) analysis, and luciferase assays to study the regulation of hDPSCs by Lin28. Furthermore, gain-of-function and loss-of-function analyses were also used in explored factors regulating hDPSCs activation.

Results

The results show that Lin28 inhibited osteogenic differentiation by directly targets pre-let-7b. Through bioinformatics sequencing and dual luciferase experiments we learned that let-7b directly targets the IGF2BP2 3’UTR. Silencing of IGF2BP2 showed a similar biological effect as overexpression of let-7b. Overexpression of IGF2BP2 counteracted the differentiation-promoting effects produced by let-7b overexpression.

Discussion/conclusions

In conclusion, the RNA-binding protein Lin28 regulates osteogenic differentiation of hDPSCs by inhibiting let-7 miRNA maturation. And mature let-7b directly regulated the expression of IGF2BP2 by targeting the 3’UTR region of IGF2BP2 mRNA thus further inhibiting the differentiation of hDPSCs.

目的Lin28基因在特定条件下的激活可促进组织损伤的修复。然而,条件性表达Lin28是否有助于受损牙髓组织的恢复仍是未知数。材料和方法 我们采用乙炔基-2ʹ-脱氧尿苷(EdU)掺入实验、RNA-蛋白免疫沉淀(RIP)分析和荧光素酶实验等技术来研究Lin28对hDPSCs的调控。结果表明,Lin28通过直接靶向pre-let-7b抑制成骨分化。通过生物信息学测序和双荧光素酶实验,我们发现let-7b直接靶向IGF2BP2的3'UTR。沉默IGF2BP2显示出与过表达let-7b相似的生物效应。讨论/结论综上所述,RNA结合蛋白Lin28通过抑制let-7 miRNA的成熟来调节hDPSCs的成骨分化。而成熟的let-7b通过靶向IGF2BP2 mRNA的3'UTR区域直接调控IGF2BP2的表达,从而进一步抑制了hDPSCs的分化。
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引用次数: 0
Bridging the gap between omics research and dental practice. 弥合 omics 研究与牙科实践之间的差距。
IF 3 Q2 Dentistry Pub Date : 2024-03-04 DOI: 10.1038/s41405-024-00199-3
S Kabbashi, I A Roomaney, M Chetty

Aim: The burgeoning field of omics research has witnessed exponential growth in both medicine and dentistry. However, despite more than a decade of advancements, clinical dentistry, particularly in Low- and Middle-Income Countries (LMICs), has seen limited progress in integrating omics-based approaches into routine practice. This review aims to provide a comprehensive overview of the integration of omics approaches in dentistry, focusing on the challenges and opportunities for translating research findings into clinical practice.

Methods: we conducted a literature review using key databases to provide a brief overview of the history of genomics in dentistry. Additionally, we summarised recent breakthroughs in omics relevant to oral health practitioners, emphasising the inadequate translation of omics research into clinical practice.

Results: Despite significant growth in omics research in both medicine and dentistry, its translation into routine clinical practice in dentistry remains limited. We summarise recent breakthroughs in omics and highlight the gap between research advancements and clinical implementation.

Discussion and conclusion: The integration of omics approaches holds promise for enhancing diagnostics, personalised treatment strategies, and preventive measures in dental practice, ushering in a new era of precision oral healthcare. However, several challenges, including infrastructure limitations, cost-effectiveness, and education gaps, hinder the widespread adoption of omics-based approaches in clinical dentistry. A strong commitment to transforming dentistry is required to embrace this transition. This shift has the potential to revolutionise oral healthcare by advancing precision diagnostics and treatment strategies tailored to individual patient needs.

目的:方兴未艾的全息研究领域在医学和牙科领域都取得了指数级增长。然而,尽管经过十多年的发展,临床牙科,尤其是中低收入国家(LMICs)的临床牙科在将基于全局观念的方法融入日常实践方面进展有限。本综述旨在全面概述在牙科中整合全息方法的情况,重点关注将研究成果转化为临床实践所面临的挑战和机遇。方法:我们利用主要数据库进行了文献综述,简要概述了基因组学在牙科中的应用历史。此外,我们还总结了近期与口腔卫生从业人员相关的奥米克斯突破,强调了奥米克斯研究尚未充分转化为临床实践的问题:结果:尽管医学和口腔医学领域的全局组学研究都取得了长足的发展,但将其转化为口腔医学常规临床实践的程度仍然有限。我们总结了最近在全息研究方面取得的突破,并强调了研究进展与临床实施之间的差距:讨论和结论:整合全息方法有望加强牙科实践中的诊断、个性化治疗策略和预防措施,开创口腔精准医疗的新时代。然而,包括基础设施限制、成本效益和教育差距在内的一些挑战阻碍了基于全息技术的方法在临床牙科中的广泛应用。要迎接这一转变,就必须坚定地致力于牙科的转型。这种转变有可能通过推进精准诊断和针对患者个体需求的治疗策略,彻底改变口腔医疗保健。
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引用次数: 0
Inhibitory and preventive effects of Arnebia euchroma (Royle) Johnst. root extract on Streptococcus mutans and dental caries in rats. Arnebia euchroma (Royle) Johnst. 根提取物对大鼠变异链球菌和龋齿的抑制和预防作用。
IF 3 Q2 Dentistry Pub Date : 2024-03-02 DOI: 10.1038/s41405-024-00196-6
Zeyu Wu, Jie Song, Yangyang Zhang, Xiyu Yuan, Jin Zhao

Background: Dental caries is one of the prevalent conditions that threaten oral health. Arnebia euchroma (Royle) Johnst. root (AR) extracts exhibit anti-inflammatory, anti-cancer, and antibacterial properties. This study was designed to investigate the antibacterial impact of AR extract on Streptococcus mutans (S. mutans) UA159 and the anti-caries effect on rats.

Methods: The antibacterial activity of AR extract against S. mutans and its biofilm was determined using the bacterial sensitivity test, the biofilm sensitivity test, and the live-dead staining technique. By fluorescently tagging bacteria, the influence of bacterial adhesion rate was determined. Using a rat caries model, the anti-caries efficacy and safety of AR extract were exhaustively investigated in vivo.

Results: AR extract inhibit not only the growth of S. mutans, but also the generation of S. mutans biofilm, hence destroying and eliminating the biofilm. Moreover, AR extract were able to inhibit S. mutans' adherence to saliva-encapsulated hydroxyapatite (HAP). Further, in a rat model of caries, the AR extract is able to greatly reduce the incidence and severity of caries lesions on the smooth surface and pit and fissure of rat molars, while exhibiting excellent biosafety.

Conclusions: AR extract exhibit strong antibacterial activity against S. mutans and can lower the incidence and severity of dental cavities in rats. These findings suggest that Arnebia euchroma (Royle) Johnst. could be utilized for the prevention and treatment of dental caries.

背景:龋齿是威胁口腔健康的常见疾病之一。Arnebia euchroma (Royle) Johnst. 根(AR)提取物具有抗炎、抗癌和抗菌特性。本研究旨在探讨 AR 提取物对变异链球菌(S. mutans)UA159 的抗菌影响以及对大鼠的抗龋作用:方法:采用细菌敏感性试验、生物膜敏感性试验和活死人染色技术测定 AR 提取物对变异链球菌及其生物膜的抗菌活性。通过荧光标记细菌,确定了细菌粘附率的影响。利用大鼠龋齿模型,对 AR 提取物的抗龋功效和安全性进行了详尽的体内研究:结果:AR 提取物不仅能抑制变异杆菌的生长,还能抑制变异杆菌生物膜的生成,从而破坏和消除生物膜。此外,AR 提取物还能抑制突变杆状病毒对唾液包裹的羟基磷灰石(HAP)的粘附。此外,在大鼠龋齿模型中,AR 提取物能够大大降低大鼠臼齿光滑表面、凹陷和裂隙中龋齿病变的发生率和严重程度,同时表现出良好的生物安全性:AR提取物对突变杆状病毒具有很强的抗菌活性,能降低大鼠龋齿的发生率和严重程度。这些研究结果表明,Arnebia euchroma (Royle) Johnst.可用于预防和治疗龋齿。
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