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MORPHOLOGICAL CHARACTERIZATION AND GENETIC DIVERSITY USING THE SSR TECHNIQUE IN SOME APPLE GENOTYPES 利用SSR技术对苹果部分基因型的形态特征及遗传多样性分析
Q4 Agricultural and Biological Sciences Pub Date : 2022-12-22 DOI: 10.33045/fgr.v38.2022.09
Adina Iancu, M. Chivu
The scab is a widespread disease throughout the world causing large financial losses in apple production and needs to be controlled also by developing polygenic varieties with resistance to several races of the pathogen Venturia inaequalis by incorporation of two or more functionally different resistance genes. The studies for evidence of interest genes relating to the resistance of apple varieties to apple scab have been conducted using several types of molecular markers (SCAR, SSR, RAPD, ISSR etc.). In this experiment, using six SSR molecular markers (CH02b10, CH05e03, CH02d01, Hi07f01 and Hi07h02), was tracked the reveal of amplified fragments, corresponding to PCR products associated with resistance genes Rvi2, Rvi8, Rvi5 and Rvi11, but also the intraspecific diversity expressed at the molecular level of Romanian apple varieties, some of them having common genitors. The position of the amplified fragments on the agarose gel for the six SSR markers was located on similar values ranges to those published in various specialized papers, the size of the amplified fragments following to be evaluated by the sequencing step and published in a new paper as an addition to the results of this study.
结痂病是一种在世界范围内广泛传播的疾病,给苹果生产造成了巨大的经济损失,还需要通过结合两种或两种以上功能不同的抗性基因,培育多基因品种,以抵抗几种致病的不均等文图里亚。利用SCAR、SSR、RAPD、ISSR等分子标记,对苹果品种抗苹果赤霉病相关感兴趣基因进行了证据研究。本实验利用6个SSR分子标记(CH02b10、CH05e03、CH02d01、Hi07f01和Hi07h02),追踪了抗性基因Rvi2、Rvi8、Rvi5和Rvi11相关PCR产物的扩增片段的揭示,以及罗马尼亚苹果品种在分子水平上表达的种内多样性,其中部分品种具有共同的亲本。6个SSR标记的扩增片段在琼脂糖凝胶上的位置与各种专业论文中发表的值范围相似,扩增片段的大小有待测序步骤评估,并作为本研究结果的补充发表在一篇新论文中。
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引用次数: 0
STUDIES ON THE APPLICATION OF HERBICIDES IN RASPBERRY CULTURE TECHNOLOGY 除草剂在覆盆子栽培技术中的应用研究
Q4 Agricultural and Biological Sciences Pub Date : 2022-12-22 DOI: 10.33045/fgr.v38.2022.17
Dumitrița Alina Amăriuței Bălan, C. Zlati, Marius Dascălu, M. Istrate
Weed control in raspberry cultivation helps to reduce the economic impact of the weed control link on the technological cycle, as well as to eliminate competition for water, nutrients and sunlight, thus allowing their growth and development in normal parameters. Cultivation, mulching and herbicide are the most practical control measures. It is necessary to know both the density of weeds and the dominant species in order to establish the strategy to reduce weeding by plowing, herbicide or integrated measures, so that weed invasion falls below the economic damage threshold (PED). The application of herbicides to reduce the degree of weeding is based on the knowledge of the weed species existing in the crop, as well as the state of coverage of the plantation. In order to establish effective measures to reduce the invasion below the economic damage threshold, it is necessary to determine the density of weeds by the method of mapping them and to establish the predominant species. The mapping action took place in the period 2020-2021 and an attempt was made to cover the entire spectrum of weeds in the plantation, then averaging by species. Following the mapping of weeds in the raspberry plantation, the presence of 22 weeds identified inside the metric frame belonging to both the dicotyledonous and monocotyledonous classes was observed, most of them coming from the first group (Capsela bursa-pastoris, Lamium amplexicaule, Cirsium arvense, Cardaria draba, Setaria glauca, Agropyron repens, Cynodon dactylon.
树莓种植中的杂草控制有助于减少杂草控制环节对技术周期的经济影响,并消除对水分、营养物质和阳光的竞争,从而使其在正常参数下生长发育。栽培、覆盖和除草剂是最实用的控制措施。为了制定通过犁地、除草剂或综合措施减少杂草的策略,使杂草入侵降至经济损失阈值(PED)以下,有必要了解杂草的密度和优势物种。使用除草剂来降低除草程度是基于对作物中现有杂草种类的了解以及种植园的覆盖状况。为了制定有效措施,将入侵减少到经济损失阈值以下,有必要通过绘制杂草密度图的方法来确定杂草的密度,并确定优势种。测绘行动发生在2020-2021年,试图覆盖种植园中的所有杂草,然后按物种平均。在对树莓种植园中的杂草进行绘图后,观察到在公制框架内发现的22种杂草属于双子叶和单子叶,其中大多数来自第一组(Capsela bursa pastoris、Lamium amplexicule、Cirsium arvense、Cardaria draba、Setaria glauca、Agropyron repens、Cynodon dactylon)。
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引用次数: 0
THE WALNUT - CONSTRAINTS AND ADVANTAGES FOR A SUSTAINABLE DEVELOPMENT 核桃可持续发展的制约与优势
Q4 Agricultural and Biological Sciences Pub Date : 2022-12-22 DOI: 10.33045/fgr.v38.2022.01
Ghizlane Kabiri, S. Bouda, S. Ennahli, Hannine Hafida
The Persian walnut or English walnut (Juglans regia L.) is a commonly grown species for nut production and noble wood. The nut is one of the oldest food and traditional medicine sources. The native and commercial walnut genotypes present a large diversity that differ widely in nut productivity and quality. However, genetic erosion poses a serious threat to this tree. Several researches of walnut genetic diversity are being carried out utilizing morphological, biochemical, and molecular approaches in order to select superior walnut cultivars of different agroclimatic areas to increase nut production and quality. Genetic resource evaluation and agrodiversity conservation have a major role in ensuring food security for future generations through a continuous supply of new rootstocks and improved cultivars.
波斯胡桃或英国胡桃(Juglans regia L.)是一种常见的坚果生产和高级木材品种。坚果是最古老的食物和传统药物来源之一。本地和商业核桃基因型呈现出巨大的多样性,在坚果生产力和质量方面差异很大。然而,基因侵蚀对这棵树构成了严重威胁。利用形态、生化和分子方法对核桃遗传多样性进行了研究,以筛选不同农业气候区的优良核桃品种,提高坚果产量和品质。遗传资源评估和农业多样性保护通过不断提供新的砧木和改良品种,在确保后代粮食安全方面发挥着重要作用。
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引用次数: 0
STUDY OF MORPHOLOGICAL DIVERSITY IN SOME PLUM GENOTYPES IN THE PEDOCLIMATIC CONDITIONS OF PITEȘTI-MĂRĂCINENI piteȘti-mĂrĂcineni土壤气候条件下一些李基因型形态多样性的研究
Q4 Agricultural and Biological Sciences Pub Date : 2022-12-22 DOI: 10.33045/fgr.v38.2022.08
M. Chivu, M. Butac
During 2020 – 2022, observations and determinations regarding the growth and fruiting at 25 plum genotypes from the Pomological Collection of RIFG Pitesti - Mărăcineni were carried out in order to identify the phenological and morphological diversity, useful to the breeding program. The variability of phenological and morphological characters has a strong genetic determinism, in correlation with the evolution of pedoclimatic conditions. In the context of climatic changes, it has been essential to understand the mechanism of interaction between the genes involved in manifesting characters and traits responsible for the adaptability and productivity of the variety and the environmental conditions. The evaluation was carried out on 100 trees (4 trees/genotype), analyzing 3,000 leaves of leaves, fruits, shoots and flowers, the working volume being over 15,000 measurements and observations. Plum is a vigorous or semi-vigorous species, but 'Centenar' and 'Zamfira' show a fairly clear difference in vigor compared to the other varieties, and this can be an advantage for these varieties when used in high-density orchards. The beginning of flowering started early in the case of two varieties ('Lama' and 'Scolduș'), medium and late for most varieties and very late for 'Grase românești', 'Vinete românești' and 'Vinete românești cl. 300', while beginning of fruit ripening occurred very early for 'Early Rivers', early in the case of 7 varieties ('Centenar', 'Tita', etc.), medium or late for 14 varieties ('Anna Spath', 'Black Diamond', 'Stanley' etc.) and very late in 'Haganta', 'Topend' and 'Renclod Violet'. Also, most of the selected CPVO descriptors were polymorphic and showed more than two phenotypes a high diversity was observed between characters regarding the flower, leaf, shoots and tree habit of the 25 studied genotypes, proving that each of them presents special characters that can be useful in future breeding programs.
在2020 - 2022年期间,对25个李基因型的生长和结果进行了观察和测定,以确定其物候和形态多样性,为育种计划提供参考。物候和形态特征的变异具有很强的遗传决定论,与气候条件的演变有关。在气候变化的背景下,有必要了解参与表现性状的基因和负责品种适应性和生产力的性状与环境条件之间的相互作用机制。评估在100棵树(4棵/基因型)上进行,分析了3000片叶片、果实、嫩枝和花,工作量超过15,000次测量和观察。李子是一种活力旺盛或半活力旺盛的品种,但'Centenar'和'Zamfira'与其他品种相比,在活力方面表现出相当明显的差异,这对于这些品种在高密度果园中使用时可能是一个优势。有两个品种(‘Lama’和‘scolducup’)的开花时间较早,大多数品种的开花时间较晚,而‘Grase românești’、‘Vinete românești’和‘Vinete românești cl’的开花时间较晚。“early Rivers”的果实成熟时间很早,有7个品种(“Centenar”、“Tita”等)的果实成熟时间早,有14个品种(“Anna Spath”、“Black Diamond”、“Stanley”等)的果实成熟时间中晚,而“Haganta”、“Topend”和“Renclod Violet”的果实成熟时间很晚。此外,大多数CPVO描述子都具有多态性,表现出两种以上的表型。25个基因型的花、叶、芽和树习性的性状之间存在高度的多样性,证明每个基因型都具有特殊的性状,可以在未来的育种计划中使用。
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引用次数: 0
IN VITRO PROPAGATION TECHNOLOGY FOR THE BLACK CHOKEBERRY ARONIA MELANOCARPA (Michx.) ELLIOT 黑草莓(密歇根州)ELLIOT的离体繁殖技术
Q4 Agricultural and Biological Sciences Pub Date : 2022-12-22 DOI: 10.33045/fgr.v38.2022.29
V. Isac, C. Plopa
The fruits of Aronia melanocarpa (Michx.) Elliot are recognized for their many benefits on the human body due to their medicinal and nutritional qualities. In order to ensure the need for propagating material recently requested by the market, an attempt was made to make the multiplication more efficient. Classical methods of multiplication have given poor results, which has led to the approach of multiplication by in vitro cultures. The varieties of interest were Nero and Melrom. The research carried out so far has established effective culture media for the initiation, multiplication and rooting phases as follows: MS mineral salts and LS vitamins with a hormonal balance consisting of GAɜ, BAP and IBA for the initiation and multiplication stages. The phytohormones GAɜ and IBA were used for rooting. The regeneration of the explants in the initiation stage reached an average of 86%. The multiplication rate was on average at Nero of 6 shoots / explant and at Melrom 12.6 shoots / explant. The rooting on the established in vitro culture media was 97% for Nero and 80.19% for Melrom. The acclimatization percentages were 92.15% for Nero and 90.41% for Melrom. In vitro propagation of Aronia melanocarpa (Michx.) Elliot has proven to be the method that gives the best results for the multiplication of this species.
黑桫椤的果实(micx .)艾略特因其药用和营养价值而被公认对人体有许多好处。为了保证市场最近对宣传材料的需求,我们尝试使乘法更有效率。经典的增殖方法结果不佳,这导致了体外培养增殖的方法。不同的兴趣是尼禄和梅洛姆。目前已确定的萌发、增殖和生根阶段的有效培养基为:MS无机盐和LS维生素,激素平衡为GA / BAP / IBA。用植物激素GA / a和IBA进行生根。萌发期外植体的再生率平均达到86%。尼禄的平均增殖率为6个芽/外植体,梅尔罗姆的平均增殖率为12.6个芽/外植体。在已建立的离体培养基上,Nero和Melrom的生根率分别为97%和80.19%。Nero和Melrom的驯化率分别为92.15%和90.41%。黑桫椤离体繁殖的研究艾略特的方法已经被证明是这个物种繁殖的最佳方法。
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引用次数: 0
IMPROVING THE ASSORTMENT WITH NEW PEAR VARIETIES, OBTAINED AT RSFG VOINEȘTI 用在RSFG VOINEȘTI获得的新梨品种改进品种组合
Q4 Agricultural and Biological Sciences Pub Date : 2022-12-22 DOI: 10.33045/fgr.v38.2022.07
Mihaiela Erculescu, N. Andreieş
The pear (Pyrus communis) breeding at the Research Station for Fruit Growing (RSFG) Voinesti, Romania began in 1950, but since 1960 it has been introduced as the main selection objective, resistance to the main diseases and pests along with other agrobiological and technological characteristics. The main source of resistance to disease and pests is some cultivated biotypes with genetic ancestry in Pyrus serotina. Over a period of more than half a century, a vast biological material was selected, which served to register, between 2004 and 2021, 7 pear varieties: 'Corina' (2004), 'Orizont' (2004), 'Tudor' (2007), 'Romcor' (2009), 'Cristal' (2009), 'Aroma' and 'Andrei' (2021), who cumulated in their genotype the most qualities established for selection. There are also many perspective selections in the test cultures field trial.
罗马尼亚Voinesti水果生长研究站(RSFG)的梨(Pyrus communis)育种始于1950年,但自1960年以来,它已作为主要选择目标,对主要病虫害的抗性以及其他农业生物学和技术特性进行了引进。梨对病虫害的抗性主要来源于具有遗传祖先的栽培型。在半个多世纪的时间里,我们选择了大量的生物材料,并在2004年至2021年期间登记了7个梨品种:“Corina”(2004年)、“Orizont”(2004年)、“Tudor”(2007年)、“Romcor”(2009年)、“Cristal”(2009年)、“Aroma”和“Andrei”(2021年),这些品种在其基因型中积累了最适合选择的品质。在测试文化现场试验中也有许多视角选择。
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引用次数: 0
ASSESSMENT OF SWEET CHERRY CULTIVARS OBTAINED AT RESEARCH STATION FOR FRUIT GROWING IASI, ROMANIA 罗马尼亚IASI果树栽培研究站甜樱桃品种评价
Q4 Agricultural and Biological Sciences Pub Date : 2022-12-22 DOI: 10.33045/fgr.v38.2022.06
E. Iurea, S. Sîrbu, M. Corneanu, I. Golache, Iulia Mineață, I. Ungureanu
The aim of this paper is to present valuable features of sweet cherry cultivars with different maturation ages of the fruits approved at RSFG Iasi between 2017 to 2021. Due to its fruits ripening age being earlier than other fruit-growing species (beginning with May), the cherry represents the first link in the annual chain of fruits production. Analysing the bloom phenophase, it was noticed that the cultivars Margonia and Croma bloom period was about two weeks later than the other cultivars. The maturation age of the fruits was spread over 57 days, starting on May the 21st for cultivar 'Cătălina', ending on July 16th for cultivar 'George'. Statistically, in terms of fruits weight (g) and equatorial diameter (mm), cultivars 'Alexus' (9.4 g and 26.6 mm), 'Elaiaşi' (8.8 g and 25.4 mm), 'Croma' (8.0 g and 24.2 mm), 'Maria' (7.8 g and 23.7 mm) and 'Tereza' (7.8 g and 25.4 mm) were highlighted with superior significant differences in comparison with the control cultivar 'Van' (7.4 g and 25.2 mm). In terms of the fruits content in soluble dry substance, cultivars 'Tereza' (19.2%), 'Alexus' (18.6%), 'Maria' (17.8%) and 'Cătălina' (17.8%) recorded superior significant values in comparison with the control cultivar 'Van' (17.7%). All the studied cultivars presented superior resistance to the fruits cracking phenomenon in comparison with the control cultivar 'Van' (41.8%) recording values below 16%.
本文的目的是介绍2017 - 2021年在RSFG Iasi批准的果实不同成熟年龄的甜樱桃品种的有价值特征。由于它的果实成熟年龄比其他水果种植物种早(从5月开始),樱桃代表了年度水果生产链的第一个环节。通过对开花物候期的分析,发现Margonia和Croma的开花时间比其他品种晚2周左右。果实的成熟期为57天,从5月21日开始,品种‘c t丽娜’,到7月16日结束,品种‘乔治’。统计上,在果实重量(g)和赤道直径(mm)方面,品种‘Alexus’(9.4 g和26.6 mm)、‘elaia’(8.8 g和25.4 mm)、‘Croma’(8.0 g和24.2 mm)、‘Maria’(7.8 g和23.7 mm)和‘Tereza’(7.8 g和25.4 mm)与对照品种‘Van’(7.4 g和25.2 mm)相比差异显著。在果实可溶性干物质含量方面,‘特蕾莎’(19.2%)、‘亚力克斯’(18.6%)、‘玛利亚’(17.8%)和‘黄麻’(17.8%)显著高于对照品种‘凡’(17.7%)。所有品种对果实开裂现象的抗性均优于对照品种Van(41.8%),记录值在16%以下。
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引用次数: 0
ASSESSMENT OF SOME PEACH-NECTARINE, APRICOT AND ALMOND ROOTSTOCKES IN NURSERY 桃油桃、杏、杏仁苗圃砧木评价
Q4 Agricultural and Biological Sciences Pub Date : 2022-12-22 DOI: 10.33045/fgr.v38.2022.30
S. Nicolae, C. Mazilu, A. Oprița, C. Gavăț
During 2019-2021 periode some autochthonous rootstocks of stone fruit were studied in at RIFG Pitești nursery: for peach and nectarine 'Adaptabil' and 'B83/8', for apricot, 'Apricor', 'Baroc', 'RoP8803001' and 'RoP8802011', for almond 'Miroper' and 'Aidared'. There were recorded: production of unrooted cuttings in mother plantation, rooting capacity using softwood cuttings, rate of rooted plants in first field of nursery and variety-rootstock interaction in second field of nursery. Besides `Adaptabil`, `Baroc` and `Miroper` new selections `RoP8803001` and `Aidared` proved good performances to be promoted in the next step: orchard behavior.
在2019-2021年期间,在RIFG Pitești苗圃研究了一些本土核果砧木:桃子和油桃的‘Adaptabil’和‘B83/8’,杏、‘Apricor’、‘Baroc’、‘RoP8803001’和‘RoP8802011’,杏仁的‘Miroper’和‘aibold’。记录了母林无根插条产量、针叶插条生根能力、苗圃第一块地生根率和苗圃第二块地品种-砧木互作情况。除了“Adaptabil”外,“Baroc”和“Miroper”的新选种“RoP8803001”和“aidare”表现良好,可以在下一步的果园行为中推广。
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引用次数: 0
PRELIMINARY RESULTS REGARDING THE BEHAVIOR OF SOME BLACKBERRIES GENOTYPES IN MOARA DOMNEASCĂ (ILFOV COUNTY) AREA CONDITIONS 关于某些黑莓基因型在MOARA DOMNEASCĂ(ILFOV县)地区条件下行为的初步结果
Q4 Agricultural and Biological Sciences Pub Date : 2022-12-22 DOI: 10.33045/fgr.v38.2022.25
M. Dogaru, Damian Dragomir, Maria-Marinela Dragnea, Ailin Moloșag
Blackberries are an important commercial fruit crop, widely grown. The blackberries contain significant amounts of polyphenol antioxidants such as anthocyanin pigments linked to potential health protection against several human diseases, so the demand is increasing. In order to identify the most suitable varieties for the Vlasia Plain area, in 2020 an experimental plot with 6 stable accessions was established. The cv. ‘Chester’, ‘Dar 8’, ‘Navaho’,‘Polar’, ‘Thornfree’, ‘Triple Crown’, were planted in a plantation scheme 3.0 m × 1.0 m. For each variety, during 2021, ripened fruits were harvested and biometric determinations have been done in the laboratory, being measured fruit weight and diameter, firmness, titratable acidity and pH, all fruit quality indicators. The preliminary results showed that blackberry varieties without thorns are larger than thorny varieties and more suitable for fresh fruit consumption, while the ‘Dar 8’ fruit are not so fragrant, their pulp is stronger, so they are more suitable for processing. The study will continue so statistical analysis can be performed
黑莓是一种重要的商业水果作物,广泛种植。黑莓含有大量的多酚抗氧化剂,如花青素色素,与潜在的健康保护有关,可以预防几种人类疾病,因此需求量正在增加。为了确定最适合瓦拉西亚平原地区的品种,在2020年建立了一个有6个稳定品种的试验田。的简历。“Chester”、“Dar 8”、“Navaho”、“Polar”、“Thornfree”、“Triple Crown”的种植面积为3.0 m × 1.0 m。在2021年期间,对每个品种的成熟果实进行了收获,并在实验室进行了生物测定,测量了果实的重量和直径、硬度、可滴定酸度和pH值,以及所有果实质量指标。初步结果表明,无刺的黑莓品种比有刺的品种体型更大,更适合作为鲜果食用,而“达8”果实没有那么香,果肉更结实,更适合加工。研究将继续进行,以便进行统计分析
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引用次数: 0
EVALUATION OF PRODUCTIVE AND QUALITY PERFORMANCES AT SOME APPLE VARIETIES CULTIVATED IN A HIGH DENSITY SYSTEM IN THE DAMBOVITA FRUIT BASIN 丹参果盆高密度栽培下若干苹果品种生产品质评价
Q4 Agricultural and Biological Sciences Pub Date : 2022-12-22 DOI: 10.33045/fgr.v38.2022.15
D. Comanescu, G. Petre
The research conducted within the Research Station for Fruit Growing Voinesti during 2019-2021 highlight the performance traits of some apple varieties grown in a high-density system which is widely extended at the private producers in the Dambovita fruit growing area. The extension and moreover the generalization of this high-density system for apple with the following abroad varieties: 'Gala', 'Red Gala', 'Pinova', 'Braeburne', 'Granny Smith', 'Jonaprince', 'Goden Delicious' grafted on 'M9' rootstock, remarked by high productivity and quality of fruits. The regular annual yields of 35-40 t/ha match the producer’s expectations and consumers too. In the same time, there are emphasized new and old apple breds from Voinesti Research Station which proved a great scab resistance and has a big potential for the high-density growing system. The option for genetic resistance of apple varieties is steadily increasing in the high density orchards due to the economic efficiency and possibility to produce ecological fruits.
2019-2021年在Voinesti水果种植研究站进行的研究突出了在高密度系统中种植的一些苹果品种的性能特征,该系统在Dambovita水果种植区的私营生产商中广泛推广。将这种高密度系统推广到以下国外品种:‘Gala’、‘Red Gala’、‘Pinova’、‘Braeburne’、‘Granny Smith’、‘jonapprins’、‘golden Delicious’,嫁接到‘M9’砧木上,以高产和高品质的果实而著名。35-40吨/公顷的常规年产量既符合生产者的期望,也符合消费者的期望。同时,Voinesti研究站重点培育的新老苹果品种具有较强的抗痂性,在高密度栽培体系中具有较大的潜力。由于经济效益和生产生态果实的可能性,在高密度果园中,苹果品种的遗传抗性选择正在稳步增加。
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引用次数: 0
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Fruit Growing Research
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