Pub Date : 2025-01-15DOI: 10.1016/j.onano.2025.100233
Sara Micheli , Caterina Piunti , Elisa Varaschin , Marianna Peditto , Maria Luz Suarez , Marco Sorgato , Elisa Cimetta
Detailed studies of cells migration are key in understanding tumors metastatic spread. We used two-photon polymerization (2PP) to create precise microdevices for studying cell migration through micro-channels at a single cell resolution. Micro-channels are designed to mimic the structure of lymphatic vessels, conduits for cell movement in vivo. Neuroblastoma (NB) and human Mesenchymal Stem Cells (MSCs) represent the main tumor and its primary metastatic site. Our results revealed distinctive behaviors of NB and MSCs, both individually and in co-culture, hinting at a tumor-suppressive role of MSCs inhibiting NB migration. Pre-exposure of MSCs to NB-derived extracellular vesicles (EVs) significantly increased their motility towards tumor cells. Our platform more effectively replicates the in vivo environment of metastatic migration, with results providing new insights into the early dissemination of NB. Such microdevices hold great promise for advancing our understanding of metastasis and aiding the development of targeted anti-cancer therapies.
{"title":"Micro-channels array device fabricated via two photon lithography for cell migration studies in Neuroblastoma metastatic dissemination","authors":"Sara Micheli , Caterina Piunti , Elisa Varaschin , Marianna Peditto , Maria Luz Suarez , Marco Sorgato , Elisa Cimetta","doi":"10.1016/j.onano.2025.100233","DOIUrl":"10.1016/j.onano.2025.100233","url":null,"abstract":"<div><div>Detailed studies of cells migration are key in understanding tumors metastatic spread. We used two-photon polymerization (2PP) to create precise microdevices for studying cell migration through micro-channels at a single cell resolution. Micro-channels are designed to mimic the structure of lymphatic vessels, conduits for cell movement in vivo. Neuroblastoma (NB) and human Mesenchymal Stem Cells (MSCs) represent the main tumor and its primary metastatic site. Our results revealed distinctive behaviors of NB and MSCs, both individually and in co-culture, hinting at a tumor-suppressive role of MSCs inhibiting NB migration. Pre-exposure of MSCs to NB-derived extracellular vesicles (EVs) significantly increased their motility towards tumor cells. Our platform more effectively replicates the in vivo environment of metastatic migration, with results providing new insights into the early dissemination of NB. Such microdevices hold great promise for advancing our understanding of metastasis and aiding the development of targeted anti-cancer therapies.</div></div>","PeriodicalId":37785,"journal":{"name":"OpenNano","volume":"22 ","pages":"Article 100233"},"PeriodicalIF":0.0,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143150134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Castration-resistant prostate cancer (CRPC) presents a formidable challenge due to its aggressiveness and limited treatment options. Loading the drug docetaxel (DTX) into liposomes is a potential alternative approach to improve its efficacy. Several studies have reported that size optimization can improve drug efficacy in other cancer models. Therefore, this study explored the potential of size-optimization of docetaxel-loaded liposomes (LDTX) to improve in vitro efficacy against CRPC. The impacts of LDTX size (<100 nm and 100–200 nm) on cellular uptake, cytotoxicity in both monolayer and three-dimensional (3D) tumor spheroid models, and anti-metastatic effects were investigated. The results showed significant cellular internalization improvement with smaller LDTX, leading to better cytotoxicity in a monolayer cell culture than with larger LDTX. Moreover, smaller liposomes enabled deep penetration into the tumor spheroid, mimicking the tumor microenvironment and effectively eradicating cancer cells inside the spheroid. Interestingly, smaller liposomes also enhanced the anti-metastatic phenotype by inhibiting cancer cell invasion. The findings demonstrate that liposomes size is crucial in enhancing the efficacy of anti-cancer drugs. Therefore, size optimization is essential for developing highly effective formulations, requiring thorough investigation to identify the optimal liposomes size for specific applications.
{"title":"Enhancing in Vitro anti-metastatic efficacy and deep penetration into tumor spheroid of docetaxel-loaded liposomes via size optimization for prostate cancer treatment","authors":"Saksorn Klibaim, Nutthanit Thumrongsiri, Natsorn Watcharadulyarat, Walailuk Chonniyom, Prattana Tanyapanyachon, Paweena Dana, Nattika Saengkrit","doi":"10.1016/j.onano.2024.100231","DOIUrl":"10.1016/j.onano.2024.100231","url":null,"abstract":"<div><div>Castration-resistant prostate cancer (CRPC) presents a formidable challenge due to its aggressiveness and limited treatment options. Loading the drug docetaxel (DTX) into liposomes is a potential alternative approach to improve its efficacy. Several studies have reported that size optimization can improve drug efficacy in other cancer models. Therefore, this study explored the potential of size-optimization of docetaxel-loaded liposomes (LDTX) to improve <em>in vitro</em> efficacy against CRPC. The impacts of LDTX size (<100 nm and 100–200 nm) on cellular uptake, cytotoxicity in both monolayer and three-dimensional (3D) tumor spheroid models, and anti-metastatic effects were investigated. The results showed significant cellular internalization improvement with smaller LDTX, leading to better cytotoxicity in a monolayer cell culture than with larger LDTX. Moreover, smaller liposomes enabled deep penetration into the tumor spheroid, mimicking the tumor microenvironment and effectively eradicating cancer cells inside the spheroid. Interestingly, smaller liposomes also enhanced the anti-metastatic phenotype by inhibiting cancer cell invasion. The findings demonstrate that liposomes size is crucial in enhancing the efficacy of anti-cancer drugs. Therefore, size optimization is essential for developing highly effective formulations, requiring thorough investigation to identify the optimal liposomes size for specific applications.</div></div>","PeriodicalId":37785,"journal":{"name":"OpenNano","volume":"22 ","pages":"Article 100231"},"PeriodicalIF":0.0,"publicationDate":"2025-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143150135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study evaluated the potential impact of VCO-loaded liposomes, particularly on the activation of tyrosinase. Optimized liposomes containing 1 % (w/w) VCO were prepared using the film deposition on carrier method, resulting in a particle size of 84.02 ± 5.00 nm and a zeta potential of -68.40 ± 2.78 mV. Encapsulation of VCO enhanced mushroom tyrosinase activity by 3-fold and exhibited lower cytotoxicity to B16-F10 cells compared to VCO alone. Moreover, a positive correlation was observed between the increase in intracellular tyrosinase activity and the concentrations of VCO (r = 0.8366) and VCO-loaded liposomes (r = 0.4794) in B16-F10 cells, while a negative correlation (r = -0.0545) was found for liposomes without VCO. A hair and eyebrow-darkening gel containing both VCO and VCO-loaded liposomes further enhanced mushroom tyrosinase activity by 283.33 ± 26.58 %. These findings suggest that VCO-loaded liposomes may serve as novel and effective nano-scale carriers for VCO in cosmetic applications.
{"title":"Evaluation of nano-sized virgin coconut oil (VCO)-loaded liposomes for enhancing mushroom and B16-F10 tyrosinase activity","authors":"Suwipa Ungphaiboon , Sutasinee Ardhanwanich , Duangkhae Maneenuan , Sirirat Pinsuwan , Pawika Mahasawat","doi":"10.1016/j.onano.2025.100232","DOIUrl":"10.1016/j.onano.2025.100232","url":null,"abstract":"<div><div>This study evaluated the potential impact of VCO-loaded liposomes, particularly on the activation of tyrosinase. Optimized liposomes containing 1 % (w/w) VCO were prepared using the film deposition on carrier method, resulting in a particle size of 84.02 ± 5.00 nm and a zeta potential of -68.40 ± 2.78 mV. Encapsulation of VCO enhanced mushroom tyrosinase activity by 3-fold and exhibited lower cytotoxicity to B16-F10 cells compared to VCO alone. Moreover, a positive correlation was observed between the increase in intracellular tyrosinase activity and the concentrations of VCO (<em>r</em> = 0.8366) and VCO-loaded liposomes (<em>r</em> = 0.4794) in B16-F10 cells, while a negative correlation (<em>r</em> = -0.0545) was found for liposomes without VCO. A hair and eyebrow-darkening gel containing both VCO and VCO-loaded liposomes further enhanced mushroom tyrosinase activity by 283.33 ± 26.58 %. These findings suggest that VCO-loaded liposomes may serve as novel and effective nano-scale carriers for VCO in cosmetic applications.</div></div>","PeriodicalId":37785,"journal":{"name":"OpenNano","volume":"22 ","pages":"Article 100232"},"PeriodicalIF":0.0,"publicationDate":"2025-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143150137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Antimicrobial resistance represents a critical global health challenge, necessitating innovative strategies to combat resistant pathogens. In this study, silver nanoparticles (AgNPs) were synthesized using honey as a bioreductant and coated with oligochitosan derived from the depolymerization of low-molecular-weight chitosan. The synthesis employed eco-friendly methods, with characterization performed via UV–Vis spectroscopy, FTIR, TEM, EDX, XRD, and LCHRMS. AgNPs synthesized with Ceiba pentandra honey exhibited an average particle size of 11.71 nm, demonstrating high antibacterial activity when coated with oligochitosan. The 10 % AgNPs-Chitosan-based hand gel sanitizer formulation achieved inhibition zones of 14.84 ± 0.40 mm against Staphylococcus aureus and 11.16 ± 0.73 mm against Pseudomonas aeruginosa. The hand gel sanitizer formulation exhibited stable pH (4.0–4.3), high resistance to syneresis at 5 °C and 40 °C, and superior antibacterial efficacy compared to alcohol-based hand gel sanitizers. Dermatological assessments confirmed the formulation's safety, and Artemia salina toxicity tests revealed the highest LC50 value (2,648.97 ppm) for AgNPs derived from C. pentandra honey. This work provides an eco-friendly, efficient method for AgNP synthesis with strong potential for biomedical and environmental applications, including their use in hand gel sanitizers to reduce pathogen transmission in various settings, contributing to the advancement of green nanotechnology.
{"title":"Chitosan-Coated silver nanoparticles with various floral honey bioreductors: A promising nonalcoholic hand gel sanitizer formulation","authors":"Saidun Fiddaroini , Kurnia Indu , Luailik Madaniyah , Suci Amalia , Aulanni'am , Moh. Farid Rahman , Akhmad Sabarudin","doi":"10.1016/j.onano.2024.100228","DOIUrl":"10.1016/j.onano.2024.100228","url":null,"abstract":"<div><div>Antimicrobial resistance represents a critical global health challenge, necessitating innovative strategies to combat resistant pathogens. In this study, silver nanoparticles (AgNPs) were synthesized using honey as a bioreductant and coated with oligochitosan derived from the depolymerization of low-molecular-weight chitosan. The synthesis employed eco-friendly methods, with characterization performed via UV–Vis spectroscopy, FTIR, TEM, EDX, XRD, and LC<img>HRMS. AgNPs synthesized with <em>Ceiba pentandra</em> honey exhibited an average particle size of 11.71 nm, demonstrating high antibacterial activity when coated with oligochitosan. The 10 % AgNPs-Chitosan-based hand gel sanitizer formulation achieved inhibition zones of 14.84 ± 0.40 mm against <em>Staphylococcus aureus</em> and 11.16 ± 0.73 mm against <em>Pseudomonas aeruginosa.</em> The hand gel sanitizer formulation exhibited stable pH (4.0–4.3), high resistance to syneresis at 5 °C and 40 °C, and superior antibacterial efficacy compared to alcohol-based hand gel sanitizers. Dermatological assessments confirmed the formulation's safety, and <em>Artemia salina</em> toxicity tests revealed the highest LC<sub>50</sub> value (2,648.97 ppm) for AgNPs derived from <em>C. pentandra</em> honey. This work provides an eco-friendly, efficient method for AgNP synthesis with strong potential for biomedical and environmental applications, including their use in hand gel sanitizers to reduce pathogen transmission in various settings, contributing to the advancement of green nanotechnology.</div></div>","PeriodicalId":37785,"journal":{"name":"OpenNano","volume":"21 ","pages":"Article 100228"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143102132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-01DOI: 10.1016/j.onano.2024.100230
Sarah Adnan Oudah , Eman B.H. Al-Khedairy
Bilastine, a second-generation antihistamine, is commonly prescribed for managing allergic rhinoconjunctivitis and urticaria due to its prolonged action. However, its therapeutic potential is constrained by poor water solubility and low oral bioavailability. This study aimed to enhance bilastine dissolution and patient compliance by formulating a nanosuspension-based orodispersible film (ODF). An anti-solvent precipitation method was employed to produce nanosuspension using different hydrophilic stabilizers (Soluplus®, Poloxamer 188, and PEG 6000). The influence of formulation parameters, such as the stabilizer ratio, the anti-solvent ratio, stirring speed, and the stabilizer type, on particle size and polydispersity index (PDI) was optimized using an experimental design approach. The optimal formulation, with a 1:1 stabilizer-to-drug ratio using Soluplus®, a 6:1 anti-solvent to solvent ratio, and a stirring rate of 820 rpm, yielded nanoparticles with a mean particle size of 83.8 nm and a narrow PDI of 0.019. This formulation also significantly enhanced the drug's dissolution rate in phosphate buffer pH 6.8, releasing 92.02% of bilastine within 90 minutes. Further characterization of the lyophilized nanoparticles using FESEM, FTIR, and XRD, confirmed their amorphous nature and drug compatibility. The optimized nanosuspension was subsequently incorporated into ODFs via the solvent-casting technique, with the optimal film formulated with a 1:1 ratio of PVA and HPMC E5 as the film-forming polymers, demonstrating a rapid disintegration time of 18 seconds and releasing 93.16% of bilastine within 6 minutes. These results confirm the successful formulation of bilastine into ODFs, significantly improving its dissolution compared to the pure drug.
{"title":"Development and characterization of bilastine nanosuspension for enhanced dissolution in orodispersible films","authors":"Sarah Adnan Oudah , Eman B.H. Al-Khedairy","doi":"10.1016/j.onano.2024.100230","DOIUrl":"10.1016/j.onano.2024.100230","url":null,"abstract":"<div><div>Bilastine, a second-generation antihistamine, is commonly prescribed for managing allergic rhinoconjunctivitis and urticaria due to its prolonged action. However, its therapeutic potential is constrained by poor water solubility and low oral bioavailability. This study aimed to enhance bilastine dissolution and patient compliance by formulating a nanosuspension-based orodispersible film (ODF). An anti-solvent precipitation method was employed to produce nanosuspension using different hydrophilic stabilizers (Soluplus®, Poloxamer 188, and PEG 6000). The influence of formulation parameters, such as the stabilizer ratio, the anti-solvent ratio, stirring speed, and the stabilizer type, on particle size and polydispersity index (PDI) was optimized using an experimental design approach. The optimal formulation, with a 1:1 stabilizer-to-drug ratio using Soluplus®, a 6:1 anti-solvent to solvent ratio, and a stirring rate of 820 rpm, yielded nanoparticles with a mean particle size of 83.8 nm and a narrow PDI of 0.019. This formulation also significantly enhanced the drug's dissolution rate in phosphate buffer pH 6.8, releasing 92.02% of bilastine within 90 minutes. Further characterization of the lyophilized nanoparticles using FESEM, FTIR, and XRD, confirmed their amorphous nature and drug compatibility. The optimized nanosuspension was subsequently incorporated into ODFs via the solvent-casting technique, with the optimal film formulated with a 1:1 ratio of PVA and HPMC E5 as the film-forming polymers, demonstrating a rapid disintegration time of 18 seconds and releasing 93.16% of bilastine within 6 minutes. These results confirm the successful formulation of bilastine into ODFs, significantly improving its dissolution compared to the pure drug.</div></div>","PeriodicalId":37785,"journal":{"name":"OpenNano","volume":"21 ","pages":"Article 100230"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143102131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-01DOI: 10.1016/j.onano.2024.100225
Brilly Andro Makalew , Syauqi Abdurrahman Abrori
This study explores a comparative analysis of PLGA nanoparticles and liposomes as potential carriers for brain cancer drug delivery, with a special focus on how material informatics enhances their design, biocompatibility, and drug release profiles to improve treatment efficacy and contribute to sustainable health outcomes.
The investigation employed a bibliometric analysis using Scopus and VOSviewer to uncover the role of material informatics in optimizing these nanocarriers. The analysis revealed that material informatics, particularly through the application of machine learning and molecular dynamics simulations, significantly optimizes the performance of both PLGA nanoparticles and liposomes.
The results highlighted distinct strengths of each nanocarrier: PLGA nanoparticles excel in biodegradability, while liposomes offer superior drug encapsulation capabilities. However, material informatics techniques bridged these enhancing drug release kinetics, stability, and biocompatibility. These improvements are crucial for effective delivery across the blood-brain barrier, a major challenge in brain cancer treatment.
The integration of computational modelling, machine learning, and high-throughput screening enabled by material informatics is shown to be a key factor in advancing the design and optimization of these nanocarriers. By leveraging these tools, researchers can develop more personalized and efficient drug delivery systems tailored to address the specific challenges of glioblastoma therapy, ultimately contributing to sustainable health outcomes
{"title":"Material informatics-driven insights into brain cancer nanocarriers: A bibliometric comparison of PLGA vs. liposomes","authors":"Brilly Andro Makalew , Syauqi Abdurrahman Abrori","doi":"10.1016/j.onano.2024.100225","DOIUrl":"10.1016/j.onano.2024.100225","url":null,"abstract":"<div><div>This study explores a comparative analysis of PLGA nanoparticles and liposomes as potential carriers for brain cancer drug delivery, with a special focus on how material informatics enhances their design, biocompatibility, and drug release profiles to improve treatment efficacy and contribute to sustainable health outcomes.</div><div>The investigation employed a bibliometric analysis using Scopus and VOSviewer to uncover the role of material informatics in optimizing these nanocarriers. The analysis revealed that material informatics, particularly through the application of machine learning and molecular dynamics simulations, significantly optimizes the performance of both PLGA nanoparticles and liposomes.</div><div>The results highlighted distinct strengths of each nanocarrier: PLGA nanoparticles excel in biodegradability, while liposomes offer superior drug encapsulation capabilities. However, material informatics techniques bridged these enhancing drug release kinetics, stability, and biocompatibility. These improvements are crucial for effective delivery across the blood-brain barrier, a major challenge in brain cancer treatment.</div><div>The integration of computational modelling, machine learning, and high-throughput screening enabled by material informatics is shown to be a key factor in advancing the design and optimization of these nanocarriers. By leveraging these tools, researchers can develop more personalized and efficient drug delivery systems tailored to address the specific challenges of glioblastoma therapy, ultimately contributing to sustainable health outcomes</div></div>","PeriodicalId":37785,"journal":{"name":"OpenNano","volume":"21 ","pages":"Article 100225"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143102129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
MRI is a powerful, non-invasive imaging technique with exceptional soft tissue contrast, requiring contrast agents to enhance sensitivity by shortening longitudinal (T1) and transverse (T2) relaxation times. While most clinical agents are chelate-based, their potential toxicity has driven the development of nanoparticle-based alternatives. Nanoparticles offer reduced toxicity, improved stability, prolonged circulation time, and better control over surface properties. Lanthanide-based nanoparticles, in particular, are promising due to their paramagnetic properties enhancing MRI contrast. The design of these nanoparticles focuses on optimizing size, shape, and colloidal stability with advances in synthesis techniques allowing for precise control over particle size, morphology, and stability to significantly influence relaxivity. Larger sizes increase r₂ values but may reduce stability, while anisotropic shapes enhance relaxivity compared to the more stable spheres. Surface modifications with functional polymers improve stability and prevent aggregation, optimizing imaging performance. As research progresses, lanthanide-based nanoparticles are poised to become crucial tools in radiology-driven cancer diagnosis and therapy, offering dual functionality for early detection, targeted treatment, and minimized off-target effects. However, these nanoparticles must be refined for tumour-specific diagnostic and therapeutic applications and undergo comprehensive safety evaluations before clinical trials.
{"title":"Recent advances in lanthanide-based nanoparticle contrast agents for magnetic resonance imaging: Synthesis, characterization, and applications","authors":"Azmi Aulia Rahmani , Qi Jia , Husein H. Bahti , Retna Putri Fauzia , Santhy Wyantuti","doi":"10.1016/j.onano.2024.100226","DOIUrl":"10.1016/j.onano.2024.100226","url":null,"abstract":"<div><div>MRI is a powerful, non-invasive imaging technique with exceptional soft tissue contrast, requiring contrast agents to enhance sensitivity by shortening longitudinal (T<sub>1</sub>) and transverse (T<sub>2</sub>) relaxation times. While most clinical agents are chelate-based, their potential toxicity has driven the development of nanoparticle-based alternatives. Nanoparticles offer reduced toxicity, improved stability, prolonged circulation time, and better control over surface properties. Lanthanide-based nanoparticles, in particular, are promising due to their paramagnetic properties enhancing MRI contrast. The design of these nanoparticles focuses on optimizing size, shape, and colloidal stability with advances in synthesis techniques allowing for precise control over particle size, morphology, and stability to significantly influence relaxivity. Larger sizes increase r₂ values but may reduce stability, while anisotropic shapes enhance relaxivity compared to the more stable spheres. Surface modifications with functional polymers improve stability and prevent aggregation, optimizing imaging performance. As research progresses, lanthanide-based nanoparticles are poised to become crucial tools in radiology-driven cancer diagnosis and therapy, offering dual functionality for early detection, targeted treatment, and minimized off-target effects. However, these nanoparticles must be refined for tumour-specific diagnostic and therapeutic applications and undergo comprehensive safety evaluations before clinical trials.</div></div>","PeriodicalId":37785,"journal":{"name":"OpenNano","volume":"21 ","pages":"Article 100226"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143102130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-01DOI: 10.1016/j.onano.2024.100223
Vishalakshi Irukuvarjula, Faye Fouladgar, Robert Powell, Emily Carney, Neda Habibi
There is a pressing need for new cell-laden, printable bioinks to mimic stiffer tissues such as cartilage, fibrotic tissue and bone. PEGDA monomers are bioinks that crosslink with light to form a viscoelastic solid, however, they lack cell adhesion properties. Here, we utilized a hybrid bioink by combining self-assembled peptide nanofibers with PEGDA for 3D printing lumens. Adult human dermal fibroblast (aHDF) cells were first seeded in peptide-laden in 2D and 3D layers and cell behavior were studied. The cell's morphology remained spheres when they were infused in the 3D hydrogel and highly aligned with 2D overlay hydrogels. HDF cells did not adhere to unmodified PEGDA lumens, however, they successfully attached and proliferated on PEGDA/peptide lumens. Moreover, HDF cells seeded on the hybrid PEGDA/peptide lumens displayed a distinct spread F-actin morphology. The results showcase the potential of peptide hydrogels in facilitating interaction of anchorage dependent cells with PEGDA structures.
{"title":"Bioprinting 3D lattice-structured lumens using polyethylene glycol diacrylate (PEGDA) combined with self-assembling peptide nanofibers as hybrid bioinks for anchorage dependent cells","authors":"Vishalakshi Irukuvarjula, Faye Fouladgar, Robert Powell, Emily Carney, Neda Habibi","doi":"10.1016/j.onano.2024.100223","DOIUrl":"10.1016/j.onano.2024.100223","url":null,"abstract":"<div><div>There is a pressing need for new cell-laden, printable bioinks to mimic stiffer tissues such as cartilage, fibrotic tissue and bone. PEGDA monomers are bioinks that crosslink with light to form a viscoelastic solid, however, they lack cell adhesion properties. Here, we utilized a hybrid bioink by combining self-assembled peptide nanofibers with PEGDA for 3D printing lumens. Adult human dermal fibroblast (aHDF) cells were first seeded in peptide-laden in 2D and 3D layers and cell behavior were studied. The cell's morphology remained spheres when they were infused in the 3D hydrogel and highly aligned with 2D overlay hydrogels. HDF cells did not adhere to unmodified PEGDA lumens, however, they successfully attached and proliferated on PEGDA/peptide lumens. Moreover, HDF cells seeded on the hybrid PEGDA/peptide lumens displayed a distinct spread F-actin morphology. The results showcase the potential of peptide hydrogels in facilitating interaction of anchorage dependent cells with PEGDA structures.</div></div>","PeriodicalId":37785,"journal":{"name":"OpenNano","volume":"21 ","pages":"Article 100223"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143102194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-01DOI: 10.1016/j.onano.2024.100224
Nor-Azmiraah Abd Jabar , Mahmud Ab Rashid Nor-Khaizura , Siti Izera Ismail , Yuet Ying Loo , Kah Hui Chong , Kousalya Padmanabhan , Shan Jiang
The biosynthesis of silver nanoparticles from ginger extract is particularly interesting due to the bioactive compounds present in ginger, which have antioxidant, antimicrobial, and anti-inflammatory properties. The study aims to optimize, characterize, and evaluate the toxicity value of the biosynthesized silver nanoparticles using Bentong ginger (Zingiber officinale) rhizome extract and commercialized ginger powder extract as reducing and capping agents. The synthesis was optimized regarding pH, silver nitrate concentration, and incubation time for better yield and stability. Additionally, biosynthesized silver nanoparticles were characterized using UV–vis spectrophotometer, X-ray diffraction, Fourier-transform Infrared, and Transmission Electron Microscope analysis. Cytotoxicity test was done using brine shrimp lethality test to determine toxicity value. The result for both Bentong ginger rhizome extract and commercialized ginger powder extract indicated that the maximum absorption of biosynthesized silver nanoparticles was 450 nm, with the most optimum pH of 11, 1 mM of silver nitrate concentration, and incubation time of 24 h. The nanoparticles were almost spherical, with an average particle size of 15.08 ± 6 nm. The analysis confirms the presence of phytochemicals in the ginger extract that aids in reducing silver ions into silver nanoparticles. Brine shrimp lethality assay showed the LC50 for AgNPs was medium toxic at 838.31 µg/mL. Although silver nanoparticles possess antimicrobial ability, the potential toxicity to human health and environmental concerns must be considered before deploying into food industries. This is the first report utilizing Bentong ginger in silver nanoparticle synthesis.
{"title":"Are Bentong ginger (Zingiber officinale) biosynthesized silver nanoparticles safe and effective? An optimization, characterization, and toxicity evaluation study","authors":"Nor-Azmiraah Abd Jabar , Mahmud Ab Rashid Nor-Khaizura , Siti Izera Ismail , Yuet Ying Loo , Kah Hui Chong , Kousalya Padmanabhan , Shan Jiang","doi":"10.1016/j.onano.2024.100224","DOIUrl":"10.1016/j.onano.2024.100224","url":null,"abstract":"<div><div>The biosynthesis of silver nanoparticles from ginger extract is particularly interesting due to the bioactive compounds present in ginger, which have antioxidant, antimicrobial, and anti-inflammatory properties. The study aims to optimize, characterize, and evaluate the toxicity value of the biosynthesized silver nanoparticles using <em>Bentong</em> ginger (<em>Zingiber officinale</em>) rhizome extract and commercialized ginger powder extract as reducing and capping agents. The synthesis was optimized regarding pH, silver nitrate concentration, and incubation time for better yield and stability. Additionally, biosynthesized silver nanoparticles were characterized using UV–vis spectrophotometer, X-ray diffraction, Fourier-transform Infrared, and Transmission Electron Microscope analysis. Cytotoxicity test was done using brine shrimp lethality test to determine toxicity value. The result for both <em>Bentong</em> ginger rhizome extract and commercialized ginger powder extract indicated that the maximum absorption of biosynthesized silver nanoparticles was 450 nm, with the most optimum pH of 11, 1 mM of silver nitrate concentration, and incubation time of 24 h. The nanoparticles were almost spherical, with an average particle size of 15.08 ± 6 nm. The analysis confirms the presence of phytochemicals in the ginger extract that aids in reducing silver ions into silver nanoparticles. Brine shrimp lethality assay showed the LC<sub>50</sub> for AgNPs was medium toxic at 838.31 µg/mL. Although silver nanoparticles possess antimicrobial ability, the potential toxicity to human health and environmental concerns must be considered before deploying into food industries. This is the first report utilizing <em>Bentong</em> ginger in silver nanoparticle synthesis.</div></div>","PeriodicalId":37785,"journal":{"name":"OpenNano","volume":"21 ","pages":"Article 100224"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143102133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-01DOI: 10.1016/j.onano.2024.100227
Mohammad Moslem Imani , Bahar Azadi , Hamid Reza Mozaffari , Mohammad Salmani Mobarakeh , Mohsen Safaei
The continuous increase of bacterial resistance in medical and industrial environments is a significant challenge due to their resistance to typical antimicrobial treatments. This study aimed to introduce a new colloidal solution containing chitosan/thyme oil/MgO Bionanocomposite with the strongest antibacterial activity. In situ synthesis method was used for the synthesis of the chitosan/thyme oil/MgO nanocomposite. Nine experiments based on the Taguchi design were created to examine the effects of three variables at three different levels. In the parameters of experiment 7 (3 mg/mL of chitosan Biopolymer, 0.5 μL/mL of thyme oil, and 6 mg/mL of MgO), the results showed that the bacterial viability was zero. The nanocomposite demonstrated enhanced structural properties and superior antibacterial activity compared to its individual components. This study showed that the synthesized nanocomposite had desirable structural properties and antibacterial activity under optimal conditions.
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