OpenNano最新文献_第5页

Anti-cancer activity of Kaempferia galanga L.–loaded polydopamine nanoparticles against colorectal cancer 山奈高良l -负载聚多巴胺纳米颗粒对结直肠癌的抗癌作用

Q2 Pharmacology, Toxicology and Pharmaceutics

OpenNano

Pub Date : 2025-03-21 DOI: 10.1016/j.onano.2025.100242

Paweena Dana , Anukul Taweechaipaisankul , Yodsathorn Wongngam , Suttipun Sungsuwan , Walailuk Chonniyom , Saksorn Klibaim , Prattana Tanyapanyachon , Monthira Rattanatayarom , Onuma Phoraksa , Udom Asawapirom , Primana Punnakitikashem , Duangporn Polpanich , Nattika Saengkrit

Kaempferia galanga L. (KGL) is an aromatic ginger that has been used as a medicinal plant. Specifically, KGL possesses anti-inflammatory, antioxidant, anti-bacterial, and anti-cancer effects. However, the key component of KGL, ethyl p‑methoxy cinnamate (EPMC), is insoluble in water, resulting in low bioavailability. Hence, a nano-drug delivery system is used to enhance KGL activities. This study aimed to employ polydopamine (PDA) nanoparticles as a carrier for KGL delivery to improve its anti-cancer activity against colorectal cancer cells. PDA- and PDA nanoparticle–loaded KGL (PDA-KGL) were synthesized using a spontaneous oxidation process. The physicochemical properties of the PDA-KGL were characterized by dynamic light scattering methods. The anti-cancer activity of PDA-KGL was evaluated in HT-29, a colorectal cancer (CRC) cell line. Average hydrodynamic sizes of PDA and PDA-KGL were 236.2 ± 1.2 and 316.6 ± 2.0 nm, respectively, and the zeta potential of PDA and PDA-KGL were -23.0 ± 0.4 and -39.5 ± 0.6 mV, respectively. The morphology of PDA-KGL observed under TEM was spherical in shape. Anti-proliferative activity was monitored in HT-29 cells using MTT and 3D tumor spheroid assays. PDA-KGL strongly inhibited cell viability of HT-29 cells compared to free KGL and PDA treatments. PDA-KGL induced apoptosis in HT-29 cells as shown by an Annexin V binding assay. In addition, PDA-KGL suppressed the invasive ability of HT-29 cells compared to free KGL or PDA, which was determined by a transwell invasion assay. Taken together, it implies that PDA-KGL might be used as a nano-drug delivery approach for colorectal cancer treatment.

山柰属高良姜（KGL）是一种芳香姜，已被用作药用植物。具体来说，KGL具有抗炎、抗氧化、抗菌和抗癌作用。然而，KGL的关键成分对甲氧基肉桂酸乙酯（EPMC）不溶于水，导致生物利用度低。因此，纳米给药系统被用来增强KGL的活性。本研究旨在利用聚多巴胺（PDA）纳米颗粒作为KGL的载体，提高其对结直肠癌细胞的抗癌活性。采用自然氧化法合成了PDA-和PDA纳米颗粒负载的KGL （PDA-KGL）。采用动态光散射方法对聚乳酸- kgl的物理化学性质进行了表征。在结直肠癌（CRC）细胞系HT-29中评价了PDA-KGL的抗癌活性。PDA和PDA- kgl的平均水动力尺寸分别为236.2±1.2和316.6±2.0 nm， zeta电位分别为-23.0±0.4和-39.5±0.6 mV。透射电镜观察到PDA-KGL的形貌为球形。使用MTT和3D肿瘤球体测定法监测HT-29细胞的抗增殖活性。与游离KGL和PDA处理相比，PDA-KGL对HT-29细胞活力有较强的抑制作用。Annexin V结合实验显示，PDA-KGL诱导HT-29细胞凋亡。此外，与游离KGL或PDA相比，PDA-KGL抑制了HT-29细胞的侵袭能力，这是通过transwell侵袭实验确定的。综上所述，这意味着PDA-KGL可能作为一种纳米药物递送方法用于结直肠癌的治疗。

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引用次数: 0

Enhanced immunomodulator activity of Carthamus tinctorius (L.) extracts, a traditional medicine using nanostructured lipid carrier approach 利用纳米结构脂质载体方法增强传统药物红花提取物的免疫调节活性

Q2 Pharmacology, Toxicology and Pharmaceutics

OpenNano

Pub Date : 2025-03-20 DOI: 10.1016/j.onano.2025.100244

Rachmat Mauludin , Insan Nurul Padjar , Neng Fisheri Kurniati , Ratna Annisa Utami

Considering the potential of Carthamus tinctorius (L.) (CT) for therapeutic purposes mainly as immunomodulator as well as the ability of nanostructured lipid carriers (NLC) to protect phytochemicals from degradation and improving their solubility, the objective of this study was design, optimization, and characterization CT-loaded NLC (CTNLC) system as an immunomodulator. The NLCs were prepared using hot homogenization techniques, which were later subjected to ultrasonication according to the Box Behnken design. The NLCs were characterized in terms of polydispersity index (PDI), particle size (PS), zeta potential (ZP), entrapment efficiency (%EE), and surface morphologies. The immunomodulatory activity was carried out by measuring phagocytic activity using carbon clearance method, organ index, antibody titres using indirect hemagglutination test and level of interferon-gamma (IFN-γ). The CT extracts (300 mg/kg), CT-NLC (100 mg/kg and 300 mg/kg) were administered orally. Levamisole (3 mg/kg) and Methylprednisolone (16 mg/kg) was used as standard drug. The selected CTNLC displayed a spherical particle with an average of ∼199.6 nm, polydispersity index of ∼0.305 and zeta potential of ∼-27.4 mV. %EE ∼74.04 % was attained. TEM images exhibited nanoparticles to be non-aggregated and in spherical shape. CTNLC treatment significantly increased the phagocytic index, antibody titres and level of IFN-γ but did not influence organ index compared to free CT. Carthamus tinctorius (L.) was successfully encapsulated into nanostructured lipid carriers. The developed NLC exhibited desirable parameters and was found to further improve immunomodulation efficacy. NLC has the potential to significantly enhance the efficacy of CT.

考虑到红花（Carthamus tinctorius， L.）（CT）主要作为免疫调节剂用于治疗目的的潜力，以及纳米结构脂质载体（NLC）保护植物化学物质免受降解和提高其溶解度的能力，本研究的目的是设计、优化和表征CT负载的NLC （CTNLC）作为免疫调节剂的系统。采用热均质技术制备NLCs，然后根据Box Behnken设计对其进行超声处理。用多分散性指数（PDI）、粒径（PS）、ζ电位（ZP）、捕获效率（%EE）和表面形貌对nlc进行了表征。通过碳清除法测定吞噬活性、器官指数、间接血凝试验测定抗体滴度和干扰素γ (IFN-γ)水平测定免疫调节活性。CT提取物（300 mg/kg）、CT- nlc （100 mg/kg、300 mg/kg）口服。左旋咪唑（3mg /kg）和甲基强的松龙（16mg /kg）作为标准药物。所选择的CTNLC显示出平均为~ 199.6 nm的球形颗粒，多分散性指数为~ 0.305，zeta电位为~ -27.4 mV。获得了%EE ~ 74.04%。TEM图像显示纳米颗粒呈非聚集球形。与游离CT相比，CTNLC治疗显著提高了吞噬指数、抗体滴度和IFN-γ水平，但对器官指数没有影响。红花（Carthamus tinctorius， L.）被成功包裹成纳米结构的脂质载体。开发的NLC具有理想的参数，并被发现进一步提高免疫调节功效。NLC具有显著提高CT疗效的潜力。

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引用次数: 0

Development and evaluation of drug-loaded niosomes fabricated by flow chemistry: A novel vortex tube reactor approach 流动化学制备载药小体的研究与评价：一种新型涡流管反应器方法

Q2 Pharmacology, Toxicology and Pharmaceutics

OpenNano

Pub Date : 2025-03-19 DOI: 10.1016/j.onano.2025.100243

Kampanart Huanbutta , Kittipat Suwanpitak , Possawee Ponlakorn , Phatsawi Sonkongdang , Isariya Kongklai , Kunlathida Luangpraditkun , Tistaya Semangoen , Tanikan Sangnim

Continuous flow technology has been increasingly applied in the fabrication of nanoparticle drug delivery systems due to its ability to provide enhanced process control, scalability, and improved uniformity in particle size. Therefore, this study aims to utilize a newly designed flow chemistry vortex tube reactor for the preparation of ketoconazole-loaded niosomes, with the goal of enhancing mixing efficiency and increasing production rates. The experiment was designed using a central composite design to investigate the effects of key preparation parameters, including total flow rate, surfactant concentration, and cholesterol content, on particle size, size distribution, zeta potential, entrapment efficiency, and drug loading percentage. The optimized formulation (Span 80 = 25 mg, Cholesterol = 50 mg) was achieved using a total flow rate of 20 mL/min. The resulting niosomes exhibited a particle size of 212.3 nm, a zeta potential of 40.2 mV, a polydispersity index of 0.282, an entrapment efficiency of 50.84 %, a drug loading of 0.58 %, and a productivity of 70.67 mg/min. Moreover, ketoconazole-loaded niosomes prepared using the newly designed flow chemistry vortex tube reactor demonstrated prolonged inhibition of Candida albicans growth compared to ketoconazole solution. A comparison with batch synthesis revealed that flow chemistry produces smaller particles with a narrower size distribution and significantly improved productivity. These findings indicate the potential for further development of the vortex reactor for industrial-scale production of nanovesicular drug delivery systems.

由于连续流技术能够提供增强的过程控制、可扩展性和颗粒尺寸的均匀性，因此它在纳米颗粒药物输送系统的制造中得到了越来越多的应用。因此，本研究旨在利用新设计的流动化学涡流管反应器制备负载酮康唑的纳米体，以提高混合效率，提高产量。本实验采用中心复合设计，考察了总流速、表面活性剂浓度和胆固醇含量等关键制备参数对样品粒径、粒径分布、zeta电位、包封效率和载药率的影响。在总流速为20 mL/min的条件下，获得最佳配方（Span 80 = 25 mg，胆固醇= 50 mg）。所得纳米粒粒径为212.3 nm， zeta电位为40.2 mV，多分散性指数为0.282，包封效率为50.84%，载药量为0.58%，产率为70.67 mg/min。此外，使用新设计的流动化学涡旋管反应器制备的负载酮康唑的ni质体与酮康唑溶液相比，对白色念珠菌的生长具有较长的抑制作用。与间歇合成的比较表明，流动化学生产的颗粒更小，粒径分布更窄，生产效率显著提高。这些发现表明涡旋反应器在工业规模生产纳米囊泡给药系统方面有进一步发展的潜力。

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引用次数: 0

Nanotechnology for effective bovine mastitis treatment: Applications in biosensors and vaccines 有效治疗牛乳腺炎的纳米技术：在生物传感器和疫苗中的应用

Q2 Pharmacology, Toxicology and Pharmaceutics

OpenNano

Pub Date : 2025-03-01 DOI: 10.1016/j.onano.2025.100238

Soheil Sadr , Babak Khoramian , Zahra Yousefsani , Ahad Jafari Rahbar Alizadeh , Abbas Rahdar , Hassan Borji

A dairy farm's most significant economic loss is caused by mastitis, one of the most common diseases in dairy cows. Mastitis occurs due to many different pathogens, such as bacteria, fungi, and algae, and antibiotics are the first line of treatment and control. Alternative methods are therefore being investigated to eradicate pathogenic microorganisms that cause mastitis. Nanotechnology is a rapidly growing field that can manufacture new materials at the nanoscale level and has tremendous potential to revolutionize the agri-food industry by offering new treatments for bovine mastitis. Considering the increasing ineffectiveness of current therapies for resistant bacteria, developing innovative products that combine nanotechnology may provide a sustainable animal health solution that can support the production of safe and high-quality food products while directly addressing a critical global problem such as antimicrobial resistance. Additionally, nanotechnology offers promising advancements in bovine mastitis research, with nanovaccines providing targeted and efficient delivery of antigens to enhance the cow's immune response and prevent the disease. On the other hand, nanobiosensors play a crucial role in the early detection of bovine mastitis, allowing for real-time monitoring of biomarkers and enabling prompt intervention, thus reducing economic losses and promoting animal welfare. Although metalic nanoparticles have the potential to offer great benefits in terms of treating bovine mastitis, there are several challenges related to their toxicity, stability, environmental safety, and production costs that need to be carefully examined and addressed in order to facilitate their use. Considering the potential of nanoparticles, these challenges can be successfully managed if applied to the right areas, which will be a new and efficient therapeutic method for improving bovine mastitis and reducing drug resistance in livestock farms, given the potential of nanoparticles. In summary, nanovaccines, nanodrugs, and nanobiosensors have great potential in managing bovine mastitis, offering targeted prevention, effective treatment, and early detection capabilities, respectively.

奶牛场最重大的经济损失是由乳腺炎造成的，乳腺炎是奶牛最常见的疾病之一。乳腺炎是由许多不同的病原体引起的，如细菌、真菌和藻类，抗生素是治疗和控制的第一线。因此，正在研究其他方法来根除引起乳腺炎的病原微生物。纳米技术是一个快速发展的领域，它可以制造纳米级的新材料，并且通过提供牛乳腺炎的新治疗方法，具有巨大的潜力来彻底改变农业食品工业。考虑到目前治疗耐药细菌的方法越来越无效，开发结合纳米技术的创新产品可能提供一种可持续的动物健康解决方案，可以支持生产安全和高质量的食品，同时直接解决诸如抗菌素耐药性等关键的全球问题。此外，纳米技术为牛乳腺炎研究提供了有希望的进展，纳米疫苗提供了有针对性和有效的抗原递送，以增强奶牛的免疫反应并预防疾病。另一方面，纳米生物传感器在牛乳腺炎的早期检测中起着至关重要的作用，可以实时监测生物标志物并进行及时干预，从而减少经济损失并促进动物福利。尽管金属纳米颗粒在治疗牛乳腺炎方面具有巨大的潜力，但在其毒性、稳定性、环境安全性和生产成本方面存在一些挑战，需要仔细检查和解决，以促进其使用。考虑到纳米颗粒的潜力，如果应用到正确的领域，这些挑战可以成功地管理，考虑到纳米颗粒的潜力，这将是改善牛乳腺炎和减少牲畜农场耐药性的一种新的有效治疗方法。总之，纳米疫苗、纳米药物和纳米生物传感器在管理牛乳腺炎方面具有巨大潜力，分别提供有针对性的预防、有效的治疗和早期发现能力。

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引用次数: 0

Biogenic selenium nanoparticles encapsulated Lactobacillus probiotics targeting colon tumorigenesis: A promising drug delivery system 靶向结肠肿瘤的益生乳杆菌包封生物硒纳米颗粒：一种有前途的药物传递系统

Q2 Pharmacology, Toxicology and Pharmaceutics

OpenNano

Pub Date : 2025-03-01 DOI: 10.1016/j.onano.2025.100239

Rehab M. Abdel-Megeed , Shaymaa A. Ismail , Mai O. Kadry

Selenium nanoparticles are less toxic compared to other selenium forms, and their biogenic production leads to improve bioavailability. However, Lactobacillus probiotics previously exhibited anticancer activity. The current study was evaluated to investigate the therapeutic potential of biogenic selenium nanoparticles that was synthesized via Lactbacillus plantarum (L. plantarum) and Lactbacillus casei (L. casei) against colorectal cancer. The induction of colorectal cancer was carried out via a single dose (5 mg/kg) of 3-methyl-1, 2-dyhydrobenzo(j)aceanthrylene [3-MBA]. After that, animals were treated using 200 µl (from stock concentration 1.0 × 10⁹ CFUs per ml) of selenium nanoparticles derived from L. casei (SeNPs-Ca) or selenium nanoparticles derived from L. plantarum (SeNPs-P) as two separate treated groups for two weeks. Colorectal tumor induction declared a significant increment in total antioxidant capacity. Meanwhile, malondialdehyde level decreased upon 3-MBA intoxication. Molecular analysis of Beclin1 as an autophagy signaling pathway revealed a remarkable reduction upon 3-MBA intoxication. Meanwhile, a significant overexpression was declared in both EGFR and MMP-9 genes post 3-MBA-induced colorectal tumor. Furthermore, a remarkable alteration was recorded in the protein expression of both p53 and TNF-α post 3-MBA injection. Both two treated groups of biogenic selenium nanoparticles declared a significant improvement in the measured parameters, with the superiority of SeNPs-Ca in modulating the altered biomarkers. Histopathological examination of colon tissue confirmed the obtained results. In conclusion, biogenic selenium nanoparticles derived from Lactobacillus probiotics could be a promising candidate as a safe and effective antitumor agent in colorectal tumor therapy and could be a promising drug delivery system targeting tumors.

与其他形式的硒相比，纳米硒的毒性更小，其生物源性的产生导致了生物利用度的提高。然而，益生乳杆菌先前显示出抗癌活性。本研究旨在探讨由植物乳杆菌（L. plantarum）和干酪乳杆菌（L. casei）合成的生物源性硒纳米颗粒对结直肠癌的治疗潜力。通过单剂量（5 mg/kg）的3-甲基- 1,2 -二氢苯并(j)乙炔[3-MBA]诱导结直肠癌。之后，分别用200µl（原液浓度1.0 × 109 CFUs / ml）干酪乳杆菌衍生的硒纳米粒子（SeNPs-Ca）或植物乳杆菌衍生的硒纳米粒子（SeNPs-P）作为两个单独的处理组，处理两周。结直肠肿瘤诱导表明总抗氧化能力显著增加。3-MBA中毒后，丙二醛水平下降。Beclin1作为自噬信号通路的分子分析显示，3-MBA中毒后Beclin1显著降低。同时，EGFR和MMP-9基因在3- mba诱导的结直肠癌后均显著过表达。此外，注射3-MBA后，p53和TNF-α的蛋白表达发生了显著变化。两组处理过的生物源硒纳米粒子在测量参数上都有显著的改善，其中SeNPs-Ca在调节改变的生物标志物方面具有优势。结肠组织病理检查证实了上述结果。综上所述，从益生乳杆菌中提取的生物源性硒纳米颗粒有望作为一种安全有效的抗肿瘤药物用于结直肠肿瘤的治疗，并有望成为一种有前景的靶向肿瘤的给药系统。

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引用次数: 0

Microbicidal mechanisms for light-activated molecular nanomachines in Mycobacterium smegmatis: A model for pathogenic bacteria 耻垢分枝杆菌中光激活分子纳米机器的杀微生物机制：一种致病菌模型

Q2 Pharmacology, Toxicology and Pharmaceutics

OpenNano

Pub Date : 2025-02-25 DOI: 10.1016/j.onano.2025.100240

Thushara Galbadage , Dongdong Liu , James M. Tour , Jeffrey D. Cirillo , Richard S. Gunasekera

There is a global health crisis of antimicrobial resistance, responsible for over a million deaths annually. Mycobacterial infections are a major contributor to this crisis, causing more deaths than any other single infectious agent. Notably, the rise of multidrug-resistant (MDR), extensively drug-resistant (XDR), and totally drug-resistant (TDR) strains of Mycobacterium tuberculosis has led to higher mortality rates and challenge all existing antibiotic regimens. Light-activated molecular nanomachines (MNMs) represent a promising class of broad-spectrum antimicrobial agents that could help counter this rise in antimicrobial resistance. Addressing a key knowledge gap, this study explores the mechanisms of action for MNMs in Mycobacterium smegmatis, a surrogate model for pathogenic mycobacteria. We show that fast-rotor MNMs significantly reduce bacterial viability, achieving up to 97 % reduction in M. smegmatis with 30 minutes of light activation when compared to non-activated MNM 1 (p < 0.0001, t = 24.55), as determined by an unpaired t-test. Using fluorescence and confocal microscopy, we also show the colocalization of MNM 1 with M. smegmatis as part of their mechanism of action. The ability to translate these observations to pathogenic mycobacteria was demonstrated by the ability of MNM 1 to kill 93.5 % of M. tuberculosis with 5 minutes of light activation when compared to non-activated MNM 1 (p < 0.0001, t = 19.24). These findings suggest that MNMs have the potential to be innovative and sustainable antimicrobial agents for the treatment of pathogenic mycobacterial infections.

全球存在抗微生物药物耐药性的健康危机，每年造成100多万人死亡。分枝杆菌感染是造成这一危机的一个主要因素，它造成的死亡人数超过任何其他单一传染因子。值得注意的是，耐多药（MDR）、广泛耐药（XDR）和完全耐药（TDR）结核分枝杆菌菌株的增加导致了更高的死亡率，并对所有现有的抗生素治疗方案构成了挑战。光激活分子纳米机器（MNMs）代表了一类有前途的广谱抗菌剂，可以帮助对抗这种抗菌素耐药性的上升。为了解决一个关键的知识缺口，本研究探讨了MNMs在耻垢分枝杆菌（致病性分枝杆菌的替代模型）中的作用机制。我们发现，快速转子MNMs显著降低了细菌活力，与未激活的MNM 1相比，在30分钟的光激活下，耻垢分枝杆菌的活性降低了97% (p <；0.0001, t = 24.55)，由非配对t检验确定。利用荧光和共聚焦显微镜，我们还发现MNM - 1与耻垢分枝杆菌的共定位是其作用机制的一部分。与未激活的MNM 1相比，MNM 1在5分钟的光激活下杀死93.5%的结核分枝杆菌，证明了将这些观察结果转化为致病性分枝杆菌的能力(p <；0.0001, t = 19.24)。这些发现表明，MNMs有潜力成为治疗致病性分枝杆菌感染的创新和可持续的抗菌药物。

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引用次数: 0

Nanoformulation, characterization, and biological activity assays of extracts of Derris trifoliata Lour, a rutin-rich mangrove plant 一种富含芦丁的红树林植物——三叶草提取物的纳米配方、表征和生物活性分析

Q2 Pharmacology, Toxicology and Pharmaceutics

OpenNano

Pub Date : 2025-02-11 DOI: 10.1016/j.onano.2025.100236

Prattana Tanyapanyachon, Walailuk Chonniyom, Kananat Naksomboon, Jakarwan Yostawonkul, Paweena Dana, Udom Asawapirom, Nattika Saengkrit

Derris trifoliata Lour (DT) is a mangrove plant species with a promising anti-inflammatory effect. However, the application of DT as a topical anti-inflammatory agent is limited due to its poor bioavailability. Here, DT extract (DTE) from Kapoe District, Ranong Province, Thailand was selected due to its highest rutin over three investigated sources. The DTE was loaded into a nanoemulsion (NE), giving 74.33 ± 0.12 % and 75.29 ± 0.51 %, encapsulation efficiency of rutin and total phenolic content (TPC), respectively. The DTE-loaded NE (DTE-NE) exhibited a nano size (90.59 ± 1.27 nm) spherical, negative charge (-33.13 ± 0.12 mV), and narrow dispersity index (0.28 ± 0.01). The in vitro release profile of rutin and TPC from DTE-NE was slower compared to those from DTE. DTE-NE displayed a superior antioxidant capacity to DTE. Compared to DTE, DTE-NE treatment potentially lowered the inflammatory mediators (nitrite, interleukin-6, and tumor necrosis factor-alpha). Moreover, the physicochemical characteristics, antioxidant and anti-inflammatory activities remained unchanged after 1 month storage. In conclusion, the present study demonstrated the possible use of DTE-NE as a topical anti-inflammatory product.

trifoliata Lour （DT）是一种具有抗炎作用的红树植物。然而，由于其生物利用度差，DT作为局部抗炎药的应用受到限制。在这里，选择来自泰国拉廊省Kapoe地区的DT提取物（DTE），因为它在三个调查来源中含有最高的芦丁。将DTE包封在纳米乳（NE）中，对芦丁和总酚含量的包封率分别为74.33±0.12%和75.29±0.51%。dte负载的NE （DTE-NE）具有纳米尺寸（90.59±1.27 nm）球形，负电荷（-33.13±0.12 mV），分散性指数（0.28±0.01）窄。与DTE相比，DTE- ne的芦丁和TPC的体外释放速度较慢。DTE- ne表现出优于DTE的抗氧化能力。与DTE相比，DTE- ne治疗可能降低炎症介质（亚硝酸盐、白细胞介素-6和肿瘤坏死因子- α）。贮藏1个月后，其理化特性、抗氧化和抗炎活性基本保持不变。总之，本研究证明了DTE-NE作为局部抗炎产品的可能性。

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引用次数: 0

Resveratrol loaded zein nanoparticles for inhibiting proliferation of osteosarcoma cells: Synthesis, characterization, release profile, and cytotoxicity 白藜芦醇载玉米蛋白纳米颗粒抑制骨肉瘤细胞增殖：合成、表征、释放谱和细胞毒性

Q2 Pharmacology, Toxicology and Pharmaceutics

OpenNano

Pub Date : 2025-02-09 DOI: 10.1016/j.onano.2025.100237

Thanida Chuacharoen , Carlos E. Astete , Cristina M. Sabliov

Resveratrol loaded zein nanoparticles (Res ZNPs) were synthesized to deliver resveratrol to bone cells for inhibiting the proliferation of osteosarcoma cells. Zein chemically cross-linked with alendronate as a bone-targeting agent using EDC and NHS chemistry was also synthesized and the cross-linking was confirmed using Fourier-Transform Infrared and Nuclear Magnetic Resonance spectroscopy. Subsequently, Res ZNPs with and without alendronate were synthesized and characterized. The particles measured 273 to 294 nm with a narrow polydispersity index, and a zeta potential of -29 to -33 mV, respectively as evaluated by dynamic light scattering. The particles showed spherical morphology imaged by transmission electron microscopy and the entrapment efficiency and loading capacity were 63.0 % and 13.6 % for Res ZNPs and 69.1 % and 21.0 % for Res ZNPs with alendronate, respectively. Furthermore, the entrapped resveratrol of both systems was released in a three-phase manner under physiological condition (phosphate-buffered saline, PBS) at 37 °C over 24 h. Both systems exhibited suppression of osteosarcoma MG-63 cell proliferation and the inhibition rate was found slightly higher for targeted, Res ZNPs with alendronate. This research suggested that Res ZNPs conjugated with alendronate could be a candidate for effective bone-targeting delivery systems to inhibit the proliferation of osteosarcoma cells.

合成了白藜芦醇载玉米蛋白纳米颗粒（Res ZNPs），将白藜芦醇输送到骨细胞中，抑制骨肉瘤细胞的增殖。用EDC和NHS化学方法合成了玉米蛋白与阿仑膦酸钠作为骨靶向剂的化学交联，并用傅里叶变换红外光谱和核磁共振光谱证实了这种交联。随后，合成和表征了含和不含阿仑膦酸钠的Res ZNPs。动态光散射结果表明，纳米粒子的多分散性指数为273 ~ 294 nm， zeta电位为-29 ~ -33 mV。经透射电镜观察，颗粒形貌呈球形，阿仑膦酸盐对Res ZNPs的包封效率为63.0%、13.6%，对Res ZNPs的包封率为69.1%、21.0%。此外，在生理条件下（磷酸盐缓冲盐水，PBS） 37°C下，两种体系的白藜芦醇在24小时内以三相方式释放。两种体系都表现出对MG-63骨肉瘤细胞增殖的抑制作用，并且发现阿仑膦酸钠对靶向Res ZNPs的抑制率略高。本研究提示，Res ZNPs结合阿仑膦酸盐可作为一种有效的骨靶向递送系统，用于抑制骨肉瘤细胞的增殖。

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引用次数: 0

Osteogenic differentiation of mesenchymal stem cells in cell-laden culture of self-assembling peptide hydrogels 自组装肽水凝胶培养中间充质干细胞的成骨分化

Q2 Pharmacology, Toxicology and Pharmaceutics

OpenNano

Pub Date : 2025-01-28 DOI: 10.1016/j.onano.2025.100235

Faye Fouladgar , Robert Powell , Vishalakshi Irukuvarjula , Akhila Joy , Xiao Li , Neda Habibi

Mesenchymal stem cell (MSC) osteogenic differentiation requires scaffolds to support multiple stages of growth and differentiation signals. Fluorenyl-9-methoxycarbonyl diphenylalanine (Fmoc-FF) peptides self-assemble to create 3D nanofibers. Here, we cultured MSC in 2D and 3D Fmoc-FF layers to support their osteogenic differentiation. The stiffness of the hydrogels was tunable between 100 and 10,000 Pa which allows precise modulation of the cellular microenvironment. Scaffold stiffness impacted cell viability which softer scaffolds (100 Pa) favored higher viability. MSC formed spheroids in 3D hydrogel and showed spread morphology in 2D overlayers. Our results demonstrate that the Fmoc-FF 3D cultures significantly enhanced osteogenic differentiation, as evidenced by increased calcium deposition, elevated phosphatase activity, and the secretion of osteocalcin. We propose that the peptides provide integrin-binding sites that activate a cytoplasmic feedback loop essential for differentiation. These findings suggest that self-assembled Fmoc-FF peptide hydrogels, is a promising platform for bone tissue engineering applications.

间充质干细胞（MSC）的成骨分化需要支架来支持多阶段的生长和分化信号。氟酰-9-甲氧羰基二苯丙氨酸（Fmoc-FF）肽自组装创建三维纳米纤维。在这里，我们在2D和3D Fmoc-FF层中培养MSC以支持其成骨分化。水凝胶的硬度在100到10,000 Pa之间可调，从而可以精确调节细胞微环境。支架刚度影响细胞活力，较软的支架（100 Pa）有利于较高的活力。骨髓间充质干细胞在三维水凝胶中形成球形，在二维复层中呈扩散形态。我们的研究结果表明，Fmoc-FF 3D培养显著增强了成骨分化，钙沉积增加，磷酸酶活性升高，骨钙素分泌增加。我们提出肽提供整合素结合位点，激活分化所必需的细胞质反馈回路。这些发现表明，自组装Fmoc-FF肽水凝胶是一个很有前景的骨组织工程应用平台。

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引用次数: 0

Carrageenan bionanocomposite films incorporating Ag and Zn-Doped CeO₂ nanoparticles for active food packaging applications 含有Ag和zn掺杂CeO 2纳米粒子的卡拉胶生物复合膜用于活性食品包装

Q2 Pharmacology, Toxicology and Pharmaceutics

OpenNano

Pub Date : 2025-01-18 DOI: 10.1016/j.onano.2025.100234

Damar Rastri Adhika , Gita Genecya , Alvin Annayya Habibah , An Naas Amalia Rahardja Putri , Ubed Sonai Fahruddin Arrozi

The increasing demand for sustainable and safe food packaging has led to the exploration of bio-based materials and advanced packaging technologies. This study investigates the incorporation of silver (Ag) and zinc (Zn) doped cerium oxide nanoparticles (CeO₂ NPs) into carrageenan-based bionanocomposite films to enhance their antimicrobial properties, mechanical strength, and hydrophobicity. The synthesis of CeO₂ NPs, doped with varying concentrations of Ag and Zn, was achieved using the green synthesis method with green tea extract as a reducing agent. Characterization techniques such as X-ray diffraction (XRD), transmission electron microscopy (TEM), and Zeta potential analysis confirmed the successful doping and stability of the nanoparticles. The bionanocomposites were evaluated for their mechanical properties, water contact angle, and antibacterial activity against Escherichia coli and Bacillus cereus. Mechanical testing revealed that the addition of CeO₂ NPs, particularly Ag-doped CeO₂ NPs, significantly improved the tensile strength and Young's modulus of the bionanocomposites. Hydrophobicity assessments showed that Zn-doped CeO₂ NPs enhanced water resistance compared to Ag-doped CeO₂ NPs, making them more suitable for food packaging applications. Zn and Ag-doped CeO₂ NPs exhibited superior antibacterial activity compared to undoped CeO₂ NPs, with 20 wt% Ag-doped NPs showing the highest antibacterial activity compared to Amoxicillin as positive control and other variations. The study concludes that Zn and Ag-doped CeO₂ NPs are promising additives for developing effective and sustainable active food packaging materials.

对可持续和安全食品包装的需求日益增长，导致了对生物基材料和先进包装技术的探索。本研究研究了将银（Ag）和锌（Zn）掺杂的氧化铈纳米粒子（CeO₂NPs）掺入卡拉胶基生物纳米复合膜中，以提高其抗菌性能、机械强度和疏水性。以绿茶提取物为还原剂，采用绿色合成法合成了不同浓度Ag和Zn掺杂的CeO₂NPs。表征技术如x射线衍射（XRD）、透射电子显微镜（TEM）和Zeta电位分析证实了纳米颗粒的成功掺杂和稳定性。研究了生物纳米复合材料的力学性能、水接触角以及对大肠杆菌和蜡样芽孢杆菌的抗菌活性。力学测试表明，添加CeO₂NPs，特别是ag掺杂的CeO₂NPs，显著提高了生物纳米复合材料的抗拉强度和杨氏模量。疏水性评价表明，与掺银的CeO 2 NPs相比，掺锌的CeO 2 NPs的耐水性增强，使其更适合食品包装应用。与未掺杂的CeO₂NPs相比，Zn和ag掺杂的CeO₂NPs表现出更好的抗菌活性，其中20% ag掺杂的CeO₂NPs与阳性对照阿莫西林和其他变量相比表现出最高的抗菌活性。研究结果表明，锌和银掺杂的CeO₂NPs是开发有效和可持续的活性食品包装材料的有前途的添加剂。

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引用次数: 0