Pub Date : 2025-01-01DOI: 10.1016/j.onano.2024.100223
Vishalakshi Irukuvarjula, Faye Fouladgar, Robert Powell, Emily Carney, Neda Habibi
There is a pressing need for new cell-laden, printable bioinks to mimic stiffer tissues such as cartilage, fibrotic tissue and bone. PEGDA monomers are bioinks that crosslink with light to form a viscoelastic solid, however, they lack cell adhesion properties. Here, we utilized a hybrid bioink by combining self-assembled peptide nanofibers with PEGDA for 3D printing lumens. Adult human dermal fibroblast (aHDF) cells were first seeded in peptide-laden in 2D and 3D layers and cell behavior were studied. The cell's morphology remained spheres when they were infused in the 3D hydrogel and highly aligned with 2D overlay hydrogels. HDF cells did not adhere to unmodified PEGDA lumens, however, they successfully attached and proliferated on PEGDA/peptide lumens. Moreover, HDF cells seeded on the hybrid PEGDA/peptide lumens displayed a distinct spread F-actin morphology. The results showcase the potential of peptide hydrogels in facilitating interaction of anchorage dependent cells with PEGDA structures.
{"title":"Bioprinting 3D lattice-structured lumens using polyethylene glycol diacrylate (PEGDA) combined with self-assembling peptide nanofibers as hybrid bioinks for anchorage dependent cells","authors":"Vishalakshi Irukuvarjula, Faye Fouladgar, Robert Powell, Emily Carney, Neda Habibi","doi":"10.1016/j.onano.2024.100223","DOIUrl":"10.1016/j.onano.2024.100223","url":null,"abstract":"<div><div>There is a pressing need for new cell-laden, printable bioinks to mimic stiffer tissues such as cartilage, fibrotic tissue and bone. PEGDA monomers are bioinks that crosslink with light to form a viscoelastic solid, however, they lack cell adhesion properties. Here, we utilized a hybrid bioink by combining self-assembled peptide nanofibers with PEGDA for 3D printing lumens. Adult human dermal fibroblast (aHDF) cells were first seeded in peptide-laden in 2D and 3D layers and cell behavior were studied. The cell's morphology remained spheres when they were infused in the 3D hydrogel and highly aligned with 2D overlay hydrogels. HDF cells did not adhere to unmodified PEGDA lumens, however, they successfully attached and proliferated on PEGDA/peptide lumens. Moreover, HDF cells seeded on the hybrid PEGDA/peptide lumens displayed a distinct spread F-actin morphology. The results showcase the potential of peptide hydrogels in facilitating interaction of anchorage dependent cells with PEGDA structures.</div></div>","PeriodicalId":37785,"journal":{"name":"OpenNano","volume":"21 ","pages":"Article 100223"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143102194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-01DOI: 10.1016/j.onano.2024.100224
Nor-Azmiraah Abd Jabar , Mahmud Ab Rashid Nor-Khaizura , Siti Izera Ismail , Yuet Ying Loo , Kah Hui Chong , Kousalya Padmanabhan , Shan Jiang
The biosynthesis of silver nanoparticles from ginger extract is particularly interesting due to the bioactive compounds present in ginger, which have antioxidant, antimicrobial, and anti-inflammatory properties. The study aims to optimize, characterize, and evaluate the toxicity value of the biosynthesized silver nanoparticles using Bentong ginger (Zingiber officinale) rhizome extract and commercialized ginger powder extract as reducing and capping agents. The synthesis was optimized regarding pH, silver nitrate concentration, and incubation time for better yield and stability. Additionally, biosynthesized silver nanoparticles were characterized using UV–vis spectrophotometer, X-ray diffraction, Fourier-transform Infrared, and Transmission Electron Microscope analysis. Cytotoxicity test was done using brine shrimp lethality test to determine toxicity value. The result for both Bentong ginger rhizome extract and commercialized ginger powder extract indicated that the maximum absorption of biosynthesized silver nanoparticles was 450 nm, with the most optimum pH of 11, 1 mM of silver nitrate concentration, and incubation time of 24 h. The nanoparticles were almost spherical, with an average particle size of 15.08 ± 6 nm. The analysis confirms the presence of phytochemicals in the ginger extract that aids in reducing silver ions into silver nanoparticles. Brine shrimp lethality assay showed the LC50 for AgNPs was medium toxic at 838.31 µg/mL. Although silver nanoparticles possess antimicrobial ability, the potential toxicity to human health and environmental concerns must be considered before deploying into food industries. This is the first report utilizing Bentong ginger in silver nanoparticle synthesis.
{"title":"Are Bentong ginger (Zingiber officinale) biosynthesized silver nanoparticles safe and effective? An optimization, characterization, and toxicity evaluation study","authors":"Nor-Azmiraah Abd Jabar , Mahmud Ab Rashid Nor-Khaizura , Siti Izera Ismail , Yuet Ying Loo , Kah Hui Chong , Kousalya Padmanabhan , Shan Jiang","doi":"10.1016/j.onano.2024.100224","DOIUrl":"10.1016/j.onano.2024.100224","url":null,"abstract":"<div><div>The biosynthesis of silver nanoparticles from ginger extract is particularly interesting due to the bioactive compounds present in ginger, which have antioxidant, antimicrobial, and anti-inflammatory properties. The study aims to optimize, characterize, and evaluate the toxicity value of the biosynthesized silver nanoparticles using <em>Bentong</em> ginger (<em>Zingiber officinale</em>) rhizome extract and commercialized ginger powder extract as reducing and capping agents. The synthesis was optimized regarding pH, silver nitrate concentration, and incubation time for better yield and stability. Additionally, biosynthesized silver nanoparticles were characterized using UV–vis spectrophotometer, X-ray diffraction, Fourier-transform Infrared, and Transmission Electron Microscope analysis. Cytotoxicity test was done using brine shrimp lethality test to determine toxicity value. The result for both <em>Bentong</em> ginger rhizome extract and commercialized ginger powder extract indicated that the maximum absorption of biosynthesized silver nanoparticles was 450 nm, with the most optimum pH of 11, 1 mM of silver nitrate concentration, and incubation time of 24 h. The nanoparticles were almost spherical, with an average particle size of 15.08 ± 6 nm. The analysis confirms the presence of phytochemicals in the ginger extract that aids in reducing silver ions into silver nanoparticles. Brine shrimp lethality assay showed the LC<sub>50</sub> for AgNPs was medium toxic at 838.31 µg/mL. Although silver nanoparticles possess antimicrobial ability, the potential toxicity to human health and environmental concerns must be considered before deploying into food industries. This is the first report utilizing <em>Bentong</em> ginger in silver nanoparticle synthesis.</div></div>","PeriodicalId":37785,"journal":{"name":"OpenNano","volume":"21 ","pages":"Article 100224"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143102133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-01DOI: 10.1016/j.onano.2024.100227
Mohammad Moslem Imani , Bahar Azadi , Hamid Reza Mozaffari , Mohammad Salmani Mobarakeh , Mohsen Safaei
The continuous increase of bacterial resistance in medical and industrial environments is a significant challenge due to their resistance to typical antimicrobial treatments. This study aimed to introduce a new colloidal solution containing chitosan/thyme oil/MgO Bionanocomposite with the strongest antibacterial activity. In situ synthesis method was used for the synthesis of the chitosan/thyme oil/MgO nanocomposite. Nine experiments based on the Taguchi design were created to examine the effects of three variables at three different levels. In the parameters of experiment 7 (3 mg/mL of chitosan Biopolymer, 0.5 μL/mL of thyme oil, and 6 mg/mL of MgO), the results showed that the bacterial viability was zero. The nanocomposite demonstrated enhanced structural properties and superior antibacterial activity compared to its individual components. This study showed that the synthesized nanocomposite had desirable structural properties and antibacterial activity under optimal conditions.
{"title":"Synthesis, characterization and antibacterial properties of chitosan/thyme oil/MgO bionanocomposite against Streptococcus mutans","authors":"Mohammad Moslem Imani , Bahar Azadi , Hamid Reza Mozaffari , Mohammad Salmani Mobarakeh , Mohsen Safaei","doi":"10.1016/j.onano.2024.100227","DOIUrl":"10.1016/j.onano.2024.100227","url":null,"abstract":"<div><div>The continuous increase of bacterial resistance in medical and industrial environments is a significant challenge due to their resistance to typical antimicrobial treatments. This study aimed to introduce a new colloidal solution containing chitosan/thyme oil/MgO Bionanocomposite with the strongest antibacterial activity. In situ synthesis method was used for the synthesis of the chitosan/thyme oil/MgO nanocomposite. Nine experiments based on the Taguchi design were created to examine the effects of three variables at three different levels. In the parameters of experiment 7 (3 mg/mL of chitosan Biopolymer, 0.5 μL/mL of thyme oil, and 6 mg/mL of MgO), the results showed that the bacterial viability was zero. The nanocomposite demonstrated enhanced structural properties and superior antibacterial activity compared to its individual components. This study showed that the synthesized nanocomposite had desirable structural properties and antibacterial activity under optimal conditions.</div></div>","PeriodicalId":37785,"journal":{"name":"OpenNano","volume":"21 ","pages":"Article 100227"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143102134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study explored agricultural waste orchid (Dendrobium sonia earsakul) stem as a sustainable material to extract nanocellulose with its extract for wound dressing applications. Cellulose isolation was performed using a green method involving synergistic pretreatment with xylanase and laccase enzymes. This was followed by isolation of cellulose nanofibers (CNF) using mechanical process. The green pretreatment demonstrated high efficiency in delignification, reduced chemical usage, and increased whiteness index to 90.84 %. The fiber showed a high crystallinity index of 72.85 %, which was confirmed by wide-angle X-ray scattering (WAXS) analysis. Transmission electron microscopy (TEM) revealed CNF with an average diameter of 10.51 ± 2.41 nm. The crude extract showed anti-inflammatory effect and antimicrobial activities against S. aureus and E. coli with minimum bactericide concentration (MBC) of 51.6 mg/mL. The cytotoxicity of the extract was evaluated using MC3T3-E1 cell line. A novel multi-layered wound dressing was developed using the CNF and the extract. The chemical composition of the membrane was confirmed by Fourier transform infrared spectroscopy. The 3D structure of the membrane was established using Synchrotron radiation X-ray tomographic microscopy. The membrane is biodegradable, non-toxic against MC3T3-E1 cells and biocompatible with 78.52 % cell viability. These findings suggest the possibility of extracting valuable chemicals from agricultural wastes such as orchid stem using green pretreatment to create an eco-friendly wound dressing.
{"title":"Green extraction and isolation of cellulose nanofibrils from orchid (Dendrobium sonia earsakul) stem for wound dressing application","authors":"Naphat Usawattanakul , Nutapat Chaisirijaroenpun , Prakit Sukyai , Udomlak Sukatta , Nisit Watthanasakphuban , Thidarat Nimchua , Phakkhananan Pakawanit , Nuntaporn Kamonsutthipaijit , Selorm Torgbo","doi":"10.1016/j.onano.2024.100229","DOIUrl":"10.1016/j.onano.2024.100229","url":null,"abstract":"<div><div>This study explored agricultural waste orchid <em>(Dendrobium sonia earsakul</em>) stem as a sustainable material to extract nanocellulose with its extract for wound dressing applications. Cellulose isolation was performed using a green method involving synergistic pretreatment with xylanase and laccase enzymes. This was followed by isolation of cellulose nanofibers (CNF) using mechanical process. The green pretreatment demonstrated high efficiency in delignification, reduced chemical usage, and increased whiteness index to 90.84 %. The fiber showed a high crystallinity index of 72.85 %, which was confirmed by wide-angle X-ray scattering (WAXS) analysis. Transmission electron microscopy (TEM) revealed CNF with an average diameter of 10.51 ± 2.41 nm. The crude extract showed anti-inflammatory effect and antimicrobial activities against <em>S. aureus</em> and <em>E. coli</em> with minimum bactericide concentration (MBC) of 51.6 mg/mL. The cytotoxicity of the extract was evaluated using MC3T3-E1 cell line. A novel multi-layered wound dressing was developed using the CNF and the extract. The chemical composition of the membrane was confirmed by Fourier transform infrared spectroscopy. The 3D structure of the membrane was established using Synchrotron radiation X-ray tomographic microscopy. The membrane is biodegradable, non-toxic against MC3T3-E1 cells and biocompatible with 78.52 % cell viability. These findings suggest the possibility of extracting valuable chemicals from agricultural wastes such as orchid stem using green pretreatment to create an eco-friendly wound dressing.</div></div>","PeriodicalId":37785,"journal":{"name":"OpenNano","volume":"22 ","pages":"Article 100229"},"PeriodicalIF":0.0,"publicationDate":"2024-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143150136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Human papillomavirus subtypes 16 and 18-associated cervical cancer is a major global health problem that affects women.
Main body
Conventional treatment approaches, including chemotherapy, immunotherapy, and gene therapy, are typically vulnerable to systemic harm, cytotoxicity, non-specificity, lack of bioavailability, poor efficacy, poor pharmacokinetics, vaginal mucosal impermeability, slow therapeutic delivery, and adverse reactions. Moreover, conventional therapeutic approaches have problems associated with biocompatibility, stability, dispersion, and the delivery of therapeutic genes into target cells. They also produce modest amounts of long-lasting antitumor immunity and have difficulty successfully targeting and eliminating cancer cells. For this reason, nanoparticles, including polymers like poly-amidoamine and polylactide-co-glycolide dendrimers, aptamers, micelles, lipid-based nanocarriers like liposomes and pegylated lipoplexes, macromolecules, and metallic nanoparticles, including silica, copper oxide, zinc oxide, iron oxide, gold, and silver, are of paramount importance to overcome the numerous drawbacks of carrying and transporting diverse types of HPV-16 and 18-caused cervical cancer therapeutic agents. This review summarizes the application of nanoscale materials to deliver various therapeutic agents to cervical cancer cells.
Conclusions
The use of nanoparticles as medical nanoscale materials during the treatment of cervical cancer helps to improve the efficacy of various therapeutic modalities, speed up the delivery process, and decrease toxicity from drugs.
{"title":"Medical nanoscale materials for virus-induced cervical cancer therapeutic modalities: For targeting delivery","authors":"Adane Adugna , Mamaru Getinet , Gashaw Azanaw Amare , Mohammed Jemal","doi":"10.1016/j.onano.2024.100221","DOIUrl":"10.1016/j.onano.2024.100221","url":null,"abstract":"<div><h3>Background</h3><div>Human papillomavirus subtypes 16 and 18-associated cervical cancer is a major global health problem that affects women.</div></div><div><h3>Main body</h3><div>Conventional treatment approaches, including chemotherapy, immunotherapy, and gene therapy, are typically vulnerable to systemic harm, cytotoxicity, non-specificity, lack of bioavailability, poor efficacy, poor pharmacokinetics, vaginal mucosal impermeability, slow therapeutic delivery, and adverse reactions. Moreover, conventional therapeutic approaches have problems associated with biocompatibility, stability, dispersion, and the delivery of therapeutic genes into target cells. They also produce modest amounts of long-lasting antitumor immunity and have difficulty successfully targeting and eliminating cancer cells. For this reason, nanoparticles, including polymers like poly-amidoamine and polylactide-co-glycolide dendrimers, aptamers, micelles, lipid-based nanocarriers like liposomes and pegylated lipoplexes, macromolecules, and metallic nanoparticles, including silica, copper oxide, zinc oxide, iron oxide, gold, and silver, are of paramount importance to overcome the numerous drawbacks of carrying and transporting diverse types of HPV-16 and 18-caused cervical cancer therapeutic agents. This review summarizes the application of nanoscale materials to deliver various therapeutic agents to cervical cancer cells.</div></div><div><h3>Conclusions</h3><div>The use of nanoparticles as medical nanoscale materials during the treatment of cervical cancer helps to improve the efficacy of various therapeutic modalities, speed up the delivery process, and decrease toxicity from drugs.</div></div>","PeriodicalId":37785,"journal":{"name":"OpenNano","volume":"21 ","pages":"Article 100221"},"PeriodicalIF":0.0,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142698616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Cancer is a significant global health issue, with rising prevalence and mortality rates demanding urgent attention. The World Health Organization emphasizes the need for effective prevention, early detection, and treatment strategies to address this public health challenge. Current treatment modalities, including surgery, hormonal therapy, immunotherapy, radiation therapy, and chemotherapy, are often associated with considerable side effects and high costs. This study investigates the biosynthesis of silver nanoparticles using Acalypha indica L. (AgNPs), a medicinal plant recognized for its therapeutic benefits, as a potential cancer treatment with minimal side effects and a lower risk of drug resistance. AgNPs exhibit anti-inflammatory properties and the ability to inhibit angiogenesis while counteracting drug resistance mechanisms. Moreover, the use of chitosan as a coating on AgNPs (AgNPs-Chit) enhances their stability and specificity toward cancer cells, thereby improving their anticancer efficacy. Characterization of the synthesized AgNPs was conducted using various techniques, including UV-Vis spectroscopy, X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), particle size analysis (PSA), and transmission electron microscopy (TEM), confirming the successful synthesis, stability, and spherical morphology of the nanoparticles, with an average diameter of 7 nm and a zeta potential of −24.51 mV. In vitro cytotoxicity testing showed that AgNPs-Chit exhibited stronger anticancer activity against T47D cells with an IC50 value of 173 µg/mL, compared to AgNPs (IC50 244 µg/mL) and the Acalypha indica L extract (IC50 826 µg/mL). When compared to the control, treatments with AgNPs-Chit, AgNPs, and the plant extract demonstrated statistically significant differences (∗p < 0.05, ∗∗p < 0.01). These results indicate that the modification of AgNPs with chitosan (AgNPs-Chit) significantly enhances anticancer efficacy compared to both AgNPs and Acalypha indica L. extract. The modification with AgNPs increased anticancer efficiency by 338%, while AgNPs-Chit showed a 446% increase compared to the original extract, highlighting the enhanced potential of these nanoparticles in inhibiting cancer cell growth. Additionally, molecular docking studies of eight key compounds identified through LC-MS analysis (quercetin, kaempferol, catechin, indoline, 4-aminobenzoic acid, 1-(2-quinolinyl)piperazine, 3-indoleacrylic acid, and pyridine-3-carboxamide) revealed strong binding affinities to the cancer target protein 3PP0, with binding energies ranging from −9.4 to −5.9 kcal/mol, compared to doxorubicin's binding energy of −9.0 kcal/mol.
{"title":"Biosynthesis, characterization, and in-vitro anticancer effect of plant-mediated silver nanoparticles using Acalypha indica Linn: In-silico approach","authors":"Luailik Madaniyah , Saidun Fiddaroini , Elok Kamilah Hayati , Moh. Farid Rahman , Akhmad Sabarudin","doi":"10.1016/j.onano.2024.100220","DOIUrl":"10.1016/j.onano.2024.100220","url":null,"abstract":"<div><div>Cancer is a significant global health issue, with rising prevalence and mortality rates demanding urgent attention. The World Health Organization emphasizes the need for effective prevention, early detection, and treatment strategies to address this public health challenge. Current treatment modalities, including surgery, hormonal therapy, immunotherapy, radiation therapy, and chemotherapy, are often associated with considerable side effects and high costs. This study investigates the biosynthesis of silver nanoparticles using <em>Acalypha indica L.</em> (AgNPs), a medicinal plant recognized for its therapeutic benefits, as a potential cancer treatment with minimal side effects and a lower risk of drug resistance. AgNPs exhibit anti-inflammatory properties and the ability to inhibit angiogenesis while counteracting drug resistance mechanisms. Moreover, the use of chitosan as a coating on AgNPs (AgNPs-Chit) enhances their stability and specificity toward cancer cells, thereby improving their anticancer efficacy. Characterization of the synthesized AgNPs was conducted using various techniques, including UV-Vis spectroscopy, X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), particle size analysis (PSA), and transmission electron microscopy (TEM), confirming the successful synthesis, stability, and spherical morphology of the nanoparticles, with an average diameter of 7 nm and a zeta potential of −24.51 mV. In vitro cytotoxicity testing showed that AgNPs-Chit exhibited stronger anticancer activity against T47D cells with an IC<sub>50</sub> value of 173 µg/mL, compared to AgNPs (IC<sub>50</sub> 244 µg/mL) and the Acalypha indica L extract (IC<sub>50</sub> 826 µg/mL). When compared to the control, treatments with AgNPs-Chit, AgNPs, and the plant extract demonstrated statistically significant differences (∗<em>p</em> < 0.05, ∗∗<em>p</em> < 0.01). These results indicate that the modification of AgNPs with chitosan (AgNPs-Chit) significantly enhances anticancer efficacy compared to both AgNPs and <em>Acalypha indica L</em>. extract. The modification with AgNPs increased anticancer efficiency by 338%, while AgNPs-Chit showed a 446% increase compared to the original extract, highlighting the enhanced potential of these nanoparticles in inhibiting cancer cell growth. Additionally, molecular docking studies of eight key compounds identified through LC-MS analysis (quercetin, kaempferol, catechin, indoline, 4-aminobenzoic acid, 1-(2-quinolinyl)piperazine, 3-indoleacrylic acid, and pyridine-3-carboxamide) revealed strong binding affinities to the cancer target protein 3PP0, with binding energies ranging from −9.4 to −5.9 kcal/mol, compared to doxorubicin's binding energy of −9.0 kcal/mol.</div></div>","PeriodicalId":37785,"journal":{"name":"OpenNano","volume":"21 ","pages":"Article 100220"},"PeriodicalIF":0.0,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142698617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Green technologies have gained prominence, particularly in the environmentally friendly synthesis of compounds, with Selenium nanoparticles (SeNPs) emerging as a key area of interest due to their potential in drug development. The sustainable production of SeNPs using microorganisms and plants enhances their physical, chemical, and biological properties, improving their catalytic efficiency. Recent advancements in nanotechnology, especially in medicine and antimicrobial research, highlight the growing significance of green synthesis methods. These eco-conscious approaches aim to preserve natural resources while promoting sustainable nanoparticle production techniques. This review focuses on SeNPs' antimicrobial action as well as the factors that influence their green production, such as pH, temperature, and precursor concentration, all of which affect their morphology, size, and stability. SeNPs' antimicrobial activity against a diverse range of bacteria, fungi, and viruses is extensively tested, with a focus on their capacity to battle drug-resistant infections and biofilms. The mechanisms of SeNPs' antimicrobial effect are investigated, including membrane disruption, reactive oxygen species (ROS) production, and interactions with cellular components. Furthermore, their antiviral characteristics and ability to alter immune responses highlight their medicinal applications. SeNPs represent a significant advancement in green nanotechnology, offering sustainable solutions to pressing biomedical challenges, particularly in the fight against drug-resistant pathogens.
{"title":"A recent advances in antimicrobial activity of green synthesized selenium nanoparticle","authors":"Durgadevi Ravi , Bhuvaneshwari Gunasekar , Vishwanathan Kaliyaperumal , Shyamaladevi Babu","doi":"10.1016/j.onano.2024.100219","DOIUrl":"10.1016/j.onano.2024.100219","url":null,"abstract":"<div><div>Green technologies have gained prominence, particularly in the environmentally friendly synthesis of compounds, with Selenium nanoparticles (SeNPs) emerging as a key area of interest due to their potential in drug development. The sustainable production of SeNPs using microorganisms and plants enhances their physical, chemical, and biological properties, improving their catalytic efficiency. Recent advancements in nanotechnology, especially in medicine and antimicrobial research, highlight the growing significance of green synthesis methods. These eco-conscious approaches aim to preserve natural resources while promoting sustainable nanoparticle production techniques. This review focuses on SeNPs' antimicrobial action as well as the factors that influence their green production, such as pH, temperature, and precursor concentration, all of which affect their morphology, size, and stability. SeNPs' antimicrobial activity against a diverse range of bacteria, fungi, and viruses is extensively tested, with a focus on their capacity to battle drug-resistant infections and biofilms. The mechanisms of SeNPs' antimicrobial effect are investigated, including membrane disruption, reactive oxygen species (ROS) production, and interactions with cellular components. Furthermore, their antiviral characteristics and ability to alter immune responses highlight their medicinal applications. SeNPs represent a significant advancement in green nanotechnology, offering sustainable solutions to pressing biomedical challenges, particularly in the fight against drug-resistant pathogens.</div></div>","PeriodicalId":37785,"journal":{"name":"OpenNano","volume":"20 ","pages":"Article 100219"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142659519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-01DOI: 10.1016/j.onano.2024.100215
Mohammed Mehadi Hassan Chowdhury , Khadizatul Kubra , Ashekul Islam
Nanotechnology is considered one of the most advanced cancer treatment and diagnostic technologies. Due to the numerous benefits of the application of nanoparticles in cancer management, nanotechnology has proven its therapeutic and diagnostic efficiency as an alternative but promising approach against cancer. However, several limitations and challenges continue to pose obstacles to its success. Designing highly precise NPs considering physicochemical factors may lead to successful outcomes in cancer therapy. Besides, the behaviour of NPs with biomolecules in a biological system can be another potential phenomenon of NPs in biomedical applications. Here, we examined the basic aspects, obstacles, and challenges of cancer nanomedicine based on the factors associated with successful clinical outcomes in cancer management to understand this research area comprehensively.
{"title":"Fundamentals behind the success of nanotechnology in cancer treatment and diagnosis","authors":"Mohammed Mehadi Hassan Chowdhury , Khadizatul Kubra , Ashekul Islam","doi":"10.1016/j.onano.2024.100215","DOIUrl":"10.1016/j.onano.2024.100215","url":null,"abstract":"<div><div>Nanotechnology is considered one of the most advanced cancer treatment and diagnostic technologies. Due to the numerous benefits of the application of nanoparticles in cancer management, nanotechnology has proven its therapeutic and diagnostic efficiency as an alternative but promising approach against cancer. However, several limitations and challenges continue to pose obstacles to its success. Designing highly precise NPs considering physicochemical factors may lead to successful outcomes in cancer therapy. Besides, the behaviour of NPs with biomolecules in a biological system can be another potential phenomenon of NPs in biomedical applications. Here, we examined the basic aspects, obstacles, and challenges of cancer nanomedicine based on the factors associated with successful clinical outcomes in cancer management to understand this research area comprehensively.</div></div>","PeriodicalId":37785,"journal":{"name":"OpenNano","volume":"20 ","pages":"Article 100215"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142572921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-01DOI: 10.1016/j.onano.2024.100218
Yuliet Montoya , Wilson Agudelo , Alejandra Garcia-Garcia , John Bustamante
In nanotechnology, tissue engineering proposes obtaining nanomaterials of natural or synthetic origin, looking to incorporate components that exhibit a defined shape, diameter, colloidal stability, and biological identity to promote and regulate the events that occur in a cardiac cell microenvironment. This research aimed to evaluate cellular viability in an in vitro model of human fetal ventricular cardiomyocytes on interaction with gold nanoparticles biosynthesized using silk fibroin from silk fibrous waste. The Physicochemical properties were characterized by UV–visible spectroscopy, Fourier-transform infrared spectroscopy, electrokinetic potential, and scanning transmission electron microscopy. Moreover, the MTT assay was used to determine the cell viability of cardiomyocytes exposed to gold nanoparticles. The results showed that the variation of the pH of the reaction allows the synthesis of different geometries of nanoparticles with diameters between 6 and 334 nm. Furthermore, it was found that the nanoparticles with a tendency to sphericity favor the cell viability of cardiomyocytes.
{"title":"Cellular viability in an in vitro model of human ventricular cardiomyocytes (RL-14) exposed to gold nanoparticles biosynthesized using silk fibroin from silk fibrous waste","authors":"Yuliet Montoya , Wilson Agudelo , Alejandra Garcia-Garcia , John Bustamante","doi":"10.1016/j.onano.2024.100218","DOIUrl":"10.1016/j.onano.2024.100218","url":null,"abstract":"<div><div>In nanotechnology, tissue engineering proposes obtaining nanomaterials of natural or synthetic origin, looking to incorporate components that exhibit a defined shape, diameter, colloidal stability, and biological identity to promote and regulate the events that occur in a cardiac cell microenvironment. This research aimed to evaluate cellular viability in an in vitro model of human fetal ventricular cardiomyocytes on interaction with gold nanoparticles biosynthesized using silk fibroin from silk fibrous waste. The Physicochemical properties were characterized by UV–visible spectroscopy, Fourier-transform infrared spectroscopy, electrokinetic potential, and scanning transmission electron microscopy. Moreover, the MTT assay was used to determine the cell viability of cardiomyocytes exposed to gold nanoparticles. The results showed that the variation of the pH of the reaction allows the synthesis of different geometries of nanoparticles with diameters between 6 and 334 nm. Furthermore, it was found that the nanoparticles with a tendency to sphericity favor the cell viability of cardiomyocytes.</div></div>","PeriodicalId":37785,"journal":{"name":"OpenNano","volume":"20 ","pages":"Article 100218"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142659517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-01DOI: 10.1016/j.onano.2024.100216
Gerardo Garcia-Zavaleta , Daniel Mejia-Valdez , Hamed Hosseinian , Ciro A. Rodriguez , Geoffrey A. Cordell , Yadira I. Vega-Cantu , Aida Rodriguez-Garcia
Treatment of injuries to bone structure represents a significant economic burden for health care institutions and systems worldwide. The development of tissue engineering scaffolds has expanded to include the incorporation of nanotechnology platforms such as liposomes for the efficient delivery of chemotherapeutic agents. Pyrroloquinoline quinone (PQQ) is a naturally occurring quinone with antioxidant and tissue regenerative properties. In this study, the liposome-based encapsulation of PQQ was achieved by studying the effect of different downsizing methods and lipid compositions. Liposomal sonication produced stable vesicles of sizes <200 nm. The incorporation of PQQ into the liposomes and its interactions with the lipids enhanced their stability for up to four weeks and allowed sustained release for seven weeks. The results demonstrate the ability of these systems to encapsulate PQQ with high stability, efficient entrapment, and extended release profiles for their potential use in biomedicine as a delivery system for bone tissue engineering.
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