Journal of the Mechanical Behavior of Biomedical Materials最新文献_第7页

Impact of thrombus composition on virtual thrombectomy procedures using human clot analogues mechanical data 血栓成分对使用人体血栓模拟机械数据进行虚拟血栓切除术的影响。

IF 3.3 2区医学 Q2 ENGINEERING, BIOMEDICAL

Journal of the Mechanical Behavior of Biomedical Materials

Pub Date : 2025-01-02 DOI: 10.1016/j.jmbbm.2025.106886

Virginia Fregona , Giulia Luraghi , Behrooz Fereidoonnezhad , Frank J.H. Gijsen , Charles B.L.M. Majoie , Jose Felix Rodríguez Matas , Francesco Migliavacca

Endovascular thrombectomy (EVT) aims at restoring blood flow in case of acute ischemic stroke by removing the thrombus occluding a large cerebral artery. During the procedure with stent-retriever, the thrombus is captured within the device, which is then retrieved, subjecting the thrombus to several forces, potentially leading to its fragmentation. In silico studies, along with mechanical characterisation of thrombi, can enhance our understanding of the EVT, helping the development of new devices and interventional strategies. Our group previously validated a numerical approach to study EVT able to account for thrombus fragmentation. In this study, the same methodology was employed to explore the applicability of the chosen failure criterion to EVT simulations and the impact of thrombus composition on the outcome of the in silico procedure. For the first time, human clot analogues experimental data were applied to this methodology. Clot analogues of three different compositions were tested, and a material model incorporating failure was calibrated, followed by a verification analysis. Finally, the calibrated material model was used to perform EVT simulations, combining the three tested thrombus compositions with three different stent retriever models. The experimental tests confirmed a compression-tension asymmetry in the stress-strain curves, showing decreasing stiffness with increasing the red blood cell (RBC) content. Applying the resulting material models to EVT simulations demonstrated: (i) the dependency of the failure criterion on the thrombus mesh size, (ii) a greater tendency for RBC-rich thrombi to fragment, and (iii) increased difficulty in retrieving RBC-poor thrombi compared to RBC-rich thrombi.

血管内取栓（Endovascular thrombectomy， EVT）的目的是通过移除阻塞大脑大动脉的血栓来恢复急性缺血性中风患者的血流。在使用支架回收器的过程中，血栓在装置内被捕获，然后被取出，使血栓受到几种力，可能导致其碎裂。在计算机上的研究，以及血栓的机械特性，可以增强我们对EVT的理解，帮助开发新的设备和介入策略。我们的小组先前验证了一种数值方法来研究EVT能够解释血栓碎片。在本研究中，采用相同的方法来探索所选择的失效准则对EVT模拟的适用性以及血栓组成对计算机程序结果的影响。人类凝块类似物的实验数据首次应用于该方法。测试了三种不同成分的凝块类似物，并校准了包含失效的材料模型，随后进行了验证分析。最后，使用校准的材料模型进行EVT模拟，将三种测试的血栓组成与三种不同的支架回收器模型结合起来。实验结果证实了应力-应变曲线的压张不对称，随着红细胞含量的增加，刚度降低。将所得的材料模型应用于EVT模拟表明：(i)失效准则依赖于血栓网格大小，（ii）富含红细胞的血栓更倾向于碎片化，（iii）与富含红细胞的血栓相比，检索缺乏红细胞的血栓的难度增加。

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引用次数: 0

Corrigendum to “Elastic constants of biogenic calcium carbonate” (155), 106570 更正 "生物碳酸钙的弹性常数"（155），106570。

IF 3.3 2区医学 Q2 ENGINEERING, BIOMEDICAL

Journal of the Mechanical Behavior of Biomedical Materials

Pub Date : 2025-01-01 DOI: 10.1016/j.jmbbm.2024.106831

Richard Johannes Best , Andrei Sotnikov , Hagen Schmidt , Igor Zlotnikov

引用次数: 0

Scaffold geometries designed to promote bone ingrowth by enhancing mechanobiological stimulation and biotransportation - A multiobjective optimisation approach

IF 3.3 2区医学 Q2 ENGINEERING, BIOMEDICAL

Journal of the Mechanical Behavior of Biomedical Materials

Pub Date : 2024-12-31 DOI: 10.1016/j.jmbbm.2024.106883

Ben M. Ferguson , Jonathan R. Clark , Qing Li

In a tissue-engineered bone scaffold implant, the process of neo-tissue ingrowth and remodelling into hard lamellar bone occurs slowly; it typically requires a period of several months to a year (or more) to complete. This research considers the design optimisation of a scaffold's unit cell geometry for the purpose of accelerating the rate at which neo-tissue forms in the porous network of the scaffold (ingrowth), and hence, reduce the length of time to complete the bone ingrowth process. In this study, the basic structure of the scaffold is the Schwarz Primitive (P) surface unit cell, selected for its compelling biomechanical and permeability characteristics. The geometry of the scaffold is varied using two parameters (namely iso-value, k, and spatial period, a) within the surface equation defining the Schwarz P-surface unit cell. In total, sixteen different unit cell geometries are considered here with the porosity ranging from 50% to 82%.

The design objectives for the scaffold are to (i) enhance mechanobiological stimulus conditions conducive to bone apposition and (ii) enhance permeability to improve the transport of nutrients/oxygen and metabolities to and from the sites of neo-tissue formation throughout the porous scaffold. The independent design variables (

k

and

a

) of the periodic unit cell geometry are optimised to best satisfy the design objectives of appositional mechanobiological stimulus and biotransporting permeability. First, a reconstructed sheep mandible computed tomographic (CT)-based finite element (FE) analysis model is used to relate the strain energy density and mechanobiological stimulus to the design variables. Next, a computational fluid dynamics (CFD) model of a 5 × 5 × 5 unit cell scaffold is developed to relate the distributions of pressure and fluid velocity to the design variables. Then, surrogate modelling is undertaken in which bivariate cubic polynomial response surfaces are fitted to the FE and CFD analysis output data to form mathematical functions of each objective with respect to the two design variables. Finally, a multiobjective optimisation algorithm is invoked to determine the best trade-off between the competing design objectives of mechanobiological stimulus and biofluidic permeability. The novel design of the scaffold structure is anticipated to provide a better biomechanical and biotransport environment for tissue regeneration.

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引用次数: 0

Tuning the mechanical properties of alginate dialdehyde–gelatin (ADA–GEL) bioinks for bioprinting approaches by varying the degree of oxidation

IF 3.3 2区医学 Q2 ENGINEERING, BIOMEDICAL

Journal of the Mechanical Behavior of Biomedical Materials

Pub Date : 2024-12-28 DOI: 10.1016/j.jmbbm.2024.106871

Jessica Faber , Jan Hinrichsen , Anahita Ahmadi Soufivand , Hsuan-Heng Lu , Tanja Rosenberger , Emine Karakaya , Rainer Detsch , Aldo R. Boccaccini , Silvia Budday

Extrusion-based 3D bioprinting is one of the most promising and widely used technologies in bioprinting. However, the development of bioprintable, biocompatible bioinks with tailored mechanical and biological properties remains a major challenge in this field. Alginate dialdehyde–gelatin (ADA–GEL) hydrogels face these difficulties and enable to tune the mechanical properties depending on the degree of oxidation (

%

DO) of ADA. Here, we present a holistic approach for characterizing the influence of the

%

DO on the mechanical properties of ADA–GEL hydrogels under multiple loading modes, compression, tension, and torsional shear in the large-strain regime. We evaluate complex mechanical characteristics including nonlinearity, hysteresis, conditioning, and stress relaxation. We calibrate hyperelastic material models to determine the corresponding material parameters inversely. Our results confirm that decreasing the

%

DO of ionically crosslinked ADA–GEL hydrogels leads to an increase in stiffness, more distinct nonlinearity, more pronounced hysteresis, and minor preconditioning effects, while the relaxation behavior is slightly affected. The fabrication technique – molding or printing – does only slightly affect the complex mechanical properties and stress relaxation behavior. Ionically and enzymatically dual-crosslinked ADA–GEL hydrogels showed higher stresses during cyclic loading and less viscous effects during stress relaxation in all three loading modes. We conclude that the

%

DO and the crosslinking procedure are crucial parameters to tune the mechanical behavior of ADA–GEL hydrogels. Careful choice of these parameters might facilitate the fabrication of biomaterials that closely mimic the properties of native tissues for advanced tissue engineering applications.

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引用次数: 0

Aortic valve leaflet assessment to inform novel bioinspired materials: Understanding the impact of collagen fibres on the tissue's mechanical behaviour 主动脉瓣小叶评估为新型生物启发材料提供信息：了解胶原纤维对组织力学行为的影响。

IF 3.3 2区医学 Q2 ENGINEERING, BIOMEDICAL

Journal of the Mechanical Behavior of Biomedical Materials

Pub Date : 2024-12-27 DOI: 10.1016/j.jmbbm.2024.106881

Celia Hughes , Alix Whelan , David O'Reilly , Evelyn M. Campbell , Caitríona Lally

Aortic stenosis is a prevalent disease that is treated with either mechanical or bioprosthetic valve replacement devices. However, these implants can experience problems with either functionality in the case of mechanical valves or long-term durability in the case of bioprosthetic valves. To enhance next generation prosthetic valves, such as biomimetic polymeric valves, an improved understanding of the native aortic valve leaflet structure and mechanical response is required to provide much needed benchmarks for future device development. This study aims to provide such information through imaging and mechanical testing of porcine aortic valve leaflet tissue. Using second harmonic generation imaging on cleared tissue it is shown that the fibre orientations are dependent on the leaflet type (left coronary, right coronary, non-coronary), while fibre crimp is not solely dependent on either of these factors. Uniaxial tensile testing of the leaflets and their layers showed that the ventricularis layer is stiffer than the fibrosa but the fibrosa dominates the mechanical response of the whole leaflet due to its higher thickness. Overall, this work provides a detailed assessment of the native porcine aortic valve leaflets’ microstructure and mechanical response, delivering key information to aid the design and manufacture of future bioinspired valve implant devices.

主动脉瓣狭窄是一种常见的疾病，可以用机械或生物瓣膜置换装置来治疗。然而，这些植入物在机械瓣膜的情况下会遇到功能问题，或者在生物假体瓣膜的情况下会遇到长期耐用性问题。为了改进下一代人工瓣膜，如仿生聚合物瓣膜，需要更好地了解原生主动脉瓣叶结构和机械响应，为未来的设备开发提供急需的基准。本研究旨在通过猪主动脉瓣小叶组织的影像学和力学测试提供这些信息。在清除组织上使用二次谐波成像显示，纤维取向取决于小叶类型（左冠状，右冠状，非冠状），而纤维卷曲并不完全取决于这些因素中的任何一个。小叶及其层的单轴拉伸试验表明，脑室层比纤维层更硬，但纤维层因其更厚而主导整个小叶的力学响应。总的来说，这项工作提供了原生猪主动脉瓣小叶的微观结构和机械响应的详细评估，为未来生物启发瓣膜植入装置的设计和制造提供了关键信息。

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引用次数: 0

Tricuspid valve edge-to-edge repair simulations are highly sensitive to annular boundary conditions 三尖瓣边缘到边缘修复模拟对环空边界条件高度敏感。

IF 3.3 2区医学 Q2 ENGINEERING, BIOMEDICAL

Journal of the Mechanical Behavior of Biomedical Materials

Pub Date : 2024-12-22 DOI: 10.1016/j.jmbbm.2024.106879

Collin E. Haese , Vijay Dubey , Mrudang Mathur , Alison M. Pouch , Tomasz A. Timek , Manuel K. Rausch

Transcatheter edge-to-edge repair (TEER) simulations may provide insight into this novel therapeutic technology and help optimize its use. However, because of the relatively short history and technical complexity of TEER simulations, important questions remain unanswered. For example, there is no consensus on how to handle the annular boundary conditions in these simulations. In this short communication, we tested the sensitivity of such simulations to the choice of annular boundary conditions using a high-fidelity finite element model of a human tricuspid valve. Therein, we embedded the annulus among elastic springs to simulate the compliance of the perivalvular myocardium. Next, we varied the spring stiffness parametrically and explored the impact on two key measures of valve function: coaptation area and leaflet stress. Additionally, we compared our results to simulations with a pinned annulus. We found that a compliant annular boundary condition led to a TEER-induced “annuloplasty effect,” i.e., annular remodeling, as observed clinically. Moreover, softer springs led to a larger coaptation area and smaller leaflet stresses. On the other hand, pinned annular boundary conditions led to unrealistically high stresses and no “annuloplasty effect.” Furthermore, we found that the impact of the boundary conditions depended on the clip position. Our findings in this case study emphasize the importance of the annular boundary condition in tricuspid TEER simulations. Thus, we recommend that care be taken when choosing annular boundary conditions and that results from simulations using pinned boundaries should be interpreted with caution.

经导管边缘到边缘修复（TEER）模拟可以深入了解这种新型治疗技术，并有助于优化其使用。然而，由于TEER模拟的历史相对较短和技术复杂性，一些重要问题仍未得到解答。例如，在这些模拟中如何处理环形边界条件没有达成共识。在这篇简短的通信中，我们使用人体三尖瓣的高保真有限元模型测试了这种模拟对环形边界条件选择的敏感性。其中，我们将环嵌入弹性弹簧中以模拟瓣周心肌的顺应性。接下来，我们参数化地改变了弹簧刚度，并探讨了对阀功能的两个关键指标的影响：适应面积和叶应力。此外，我们将结果与固定环空的模拟结果进行了比较。我们发现，如临床观察到的那样，一个顺从的环边界条件导致teer诱导的“环成形术效应”，即环重构。此外，更软的弹簧导致更大的适应面积和更小的小叶应力。另一方面，固定环空边界条件导致不切实际的高应力，没有“环成形效应”。此外，我们发现边界条件的影响取决于夹片位置。我们在这个案例研究中的发现强调了环形边界条件在三尖瓣TEER模拟中的重要性。因此，我们建议在选择环形边界条件时要小心，并且使用固定边界的模拟结果应谨慎解释。

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引用次数: 0

Mechanical properties of the bicipital aponeurosis 肱二头肌腱膜的力学特性。

IF 3.3 2区医学 Q2 ENGINEERING, BIOMEDICAL

Journal of the Mechanical Behavior of Biomedical Materials

Pub Date : 2024-12-20 DOI: 10.1016/j.jmbbm.2024.106876

Emmanuel Ocran , Michele Oliver , Anne Agur , Amr Elmaraghy , Karen Gordon

As a biarticular muscle, the biceps brachii both supinates the forearm and flexes the elbow and shoulder, thus allowing the upper limb to perform a variety of activities of daily living (ADL). The biceps brachii originates on the coracoid apex as well as the supraglenoid tubercle and inserts on the radial tuberosity. At the distal end, the bicipital aponeurosis (BA) provides a transition of the biceps tendon into the antebrachial fascia. Previous work has reported the importance of the bicipital aponeurosis in stabilizing distal tendons. Other studies have reported the supination effect that the BA has on the forearm at the radioulnar joint, where it also protects the brachial artery and median nerve (neurovascular bundle). In addition, it has been speculated to have a proprioceptive function. However, despite the important functions fulfilled by this structure, the mechanical properties of the BA are yet to be quantified.

Mechanical properties for eight fresh frozen BA specimens (82 ± 12 years, 5 females, 5 right) were quantified using a Cellscale Biaxial (Waterloo, ON) testing machine. Three samples (approximately 7 × 7mm each) were harvested from the proximal, middle and distal regions along the length of the BA. Samples were tested on a biaxial testing machine while maintaining the alignment of the longitudinal collagen fiber orientation with the X-axis of the tester. The testing protocol included 10 preconditioning sinusoidal cycles at 9% strain, at a strain rate of 1%/s, followed by biaxial testing to a maximum strain of 12% at a strain rate of 1%/s. Young's modulus was quantified for all biaxial tests from the linear portion of the resulting stress-strain relation. Results showed that elastic modulus values were significantly greater in the longitudinal direction aligned with the collagen fibers. The outcomes of this study will provide input values for future models of distal biceps repair, thus aiding surgical planning by providing insight into the potential load sharing contributions of the BA.

作为一种双关节肌肉，肱二头肌既能使前臂旋后，又能使肘部和肩部屈曲，从而使上肢能够进行各种日常生活活动（ADL）。肱二头肌起源于喙尖和锁骨上结节并插入桡骨结节。在远端，二头肌腱膜（BA）提供二头肌腱进入肱前筋膜的过渡。以前的工作已经报道了肱二头肌腱膜在稳定远端肌腱中的重要性。其他研究报道了BA对前臂尺桡关节处的旋后作用，它也保护肱动脉和正中神经（神经血管束）。此外，据推测它还具有本体感觉功能。然而，尽管这种结构具有重要的功能，但BA的力学性能尚未被量化。采用Cellscale Biaxial （Waterloo， ON）试验机对8个新鲜冷冻BA标本（82±12年，女性5例，男性5例）的力学性能进行定量分析。沿着BA的长度从近端、中端和远端区域采集三个样本（每个约7 × 7mm）。样品在双轴试验机上进行测试，同时保持胶原纤维纵向方向与试验机的x轴对齐。试验方案包括10次预处理正弦循环，应变率为9%，应变率为1%/s，然后进行双轴测试，应变率为1%/s，最大应变为12%。杨氏模量从产生的应力-应变关系的线性部分量化所有双轴试验。结果表明，弹性模量值在与胶原纤维对齐的纵向上显著增大。本研究的结果将为未来的二头肌远端修复模型提供输入值，从而通过洞察BA的潜在负荷分担贡献来帮助手术计划。

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引用次数: 0

Automatic adjustment of dental crowns using Laplacian mesh editing 利用拉普拉斯网格编辑技术自动调整牙冠

IF 3.3 2区医学 Q2 ENGINEERING, BIOMEDICAL

Journal of the Mechanical Behavior of Biomedical Materials

Pub Date : 2024-12-20 DOI: 10.1016/j.jmbbm.2024.106878

Oliver Roffmann , Meike Stiesch , Christof Hurschler , Andreas Greuling

Currently, the restoration of missing teeth by means of dental implants is a common treatment method in dentistry. Ensuring optimal contact between teeth (occlusion) when designing the occlusal surface of an implant-supported crown is crucial for the patient. Although there are various occlusal concepts and guidelines for achieving optimised occlusion, adapting an occlusal surface is challenging. The contact points must be established in certain areas of the occlusal surface without impairing the aesthetics of the teeth and the masticatory function. A computer-aided, automated modelling approach can assist in the design process and can reduce the reliance on manual labour. This study aimed to develop a modelling approach that enables the automatic adaptation of an occlusal surface to specific occlusal concepts while preserving the natural appearance. In this study, the occlusal surface of an implant-supported crown based on a scanned first right mandibular molar was adopted. Nominal contact points were determined based on occlusal concepts by Ramfjord and Ash (RA) and Thomas (T). The shape of the occlusal surface was then adapted concerning the desired contact points using Laplacian mesh editing. The modification results were validated for different forces and crown materials (3Y-TZP and PMMA) using a finite element contact analysis. The contact analysis results showed that locations with high compressive stresses correspond with the locations of the nominal contact points. The reaction forces were more evenly distributed in PMMA crowns, due to the lower Young's modulus of PMMA compared to 3Y-TZP. Furthermore, the occlusal scheme with fewer contact points (RA) showed higher maximum reaction forces per contact area. The presented method enables the automated adaptation of an (implant-supported) crown to specific occlusal schemes, proving to be valuable in dental CAD.

目前，牙种植体修复缺牙是牙科常用的治疗方法。当设计种植体支撑冠的咬合面时，确保牙齿之间的最佳接触（咬合）对患者至关重要。虽然有各种咬合的概念和指导方针，以实现优化咬合，适应咬合表面是具有挑战性的。接触点必须建立在咬合表面的某些区域，而不损害牙齿的美观和咀嚼功能。计算机辅助的自动化建模方法可以协助设计过程，并可以减少对体力劳动的依赖。本研究旨在开发一种建模方法，使咬合表面能够自动适应特定的咬合概念，同时保持自然外观。在本研究中，采用基于扫描的右下颌第一磨牙种植支撑冠的咬合面。根据Ramfjord和Ash （RA）和Thomas (T)的咬合概念确定名义接触点。然后使用拉普拉斯网格编辑根据期望的接触点调整咬合表面的形状。通过有限元接触分析验证了不同力和不同冠材（3Y-TZP和PMMA）的修正结果。接触分析结果表明，高压应力位置与标称接触点位置相对应。与3Y-TZP相比，PMMA的杨氏模量更低，因此反作用力在PMMA冠中分布更均匀。此外，接触点较少的咬合方案显示出更高的每接触面积的最大反作用力。提出的方法能够自动适应（种植支持）冠特定的咬合方案，证明是有价值的牙科CAD。

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引用次数: 0

Mechanical characteristics of spinal cord tissue by indentation 通过压痕分析脊髓组织的机械特性

IF 3.3 2区医学 Q2 ENGINEERING, BIOMEDICAL

Journal of the Mechanical Behavior of Biomedical Materials

Pub Date : 2024-12-19 DOI: 10.1016/j.jmbbm.2024.106863

Oskar Neumann , Harsh Vardhan Surana , Stephen Melly , Paul Steinmann , Silvia Budday

The mechanical properties of brain and spinal cord tissue have proven to be extremely complex and difficult to assess. Due to the heterogeneous and ultra-soft nature of the tissue, the available literature shows a large variance in mechanical parameters derived from experiments. In this study, we performed a series of indentation experiments to systematically investigate the mechanical properties of porcine spinal cord tissue in terms of their sensitivity to indentation tip diameter, loading rate, holding time, ambient temperature along with cyclic and oscillatory dynamic loading. Our results show that spinal cord white matter tissue is more compliant than grey matter tissue with apparent moduli of 128.7 and 403.8 Pa, respectively. They show similar viscoelastic behavior with stress relaxation time constants of

τ_{1} = 1.38

s and

τ_{2} = 36.29

s for grey matter and

τ_{1} = 1.46

s and

τ_{2} = 46.10

s for white matter, while the initial peak force decreased by 54 % for grey and 59 % for white matter tissue. An increase of the applied loading rate by two orders of magnitude led to an approximate doubling of the apparent modulus for both tissue types. Thermal variations showed a decrease in apparent modulus of up to 30 % after heating from 20 to 37.0 °C. Our dynamic tests revealed a significant influence of cyclic preload on the stiffness, with a drop of up to 20 % and a relative decrease of up to 60 % after the first cycle compared to the total modulus drop after five cycles for spinal cord grey matter tissue. Oscillatory indentation experiments identified similar loss moduli for spinal cord grey and white matter tissue and a higher storage modulus for white matter tissue. This work provides systematic insights into the mechanical properties of spinal cord tissue under different loading scenarios using nanoindentation.

脑和脊髓组织的力学特性已被证明是极其复杂和难以评估的。由于组织的异质性和超柔软性，现有文献显示，从实验中得出的力学参数差异很大。在这项研究中，我们进行了一系列的压痕实验，系统地研究了猪脊髓组织的力学特性，包括压痕尖端直径、加载速率、保温时间、环境温度以及循环和振荡动态加载的敏感性。结果表明，脊髓白质组织比灰质组织更柔顺，表观模量分别为128.7和403.8 Pa。灰质的应力松弛时间常数τ1=1.38s和τ2=36.29s，白质的应力松弛时间常数τ1=1.46s和τ2=46.10s，两者表现出相似的粘弹性行为，而灰质和白质组织的初始峰值力分别下降了54%和59%。应用加载率增加两个数量级导致两种组织类型的表观模量近似加倍。热变化表明，从20°C加热到37.0°C后，表观模量下降了30%。我们的动态测试揭示了循环预载荷对刚度的显著影响，与脊髓灰质组织在五个循环后的总模量下降相比，第一个循环后的刚度下降高达20%，相对下降高达60%。振荡压痕实验表明，脊髓灰质和白质组织的损失模量相似，白质组织的储存模量更高。这项工作提供了系统的见解脊髓组织的力学性质在不同的负载情况下使用纳米压痕。

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引用次数: 0

Design, additive manufacturing, and characterization of an organ-on-chip microfluidic device for oral mucosa analogue growth 用于口腔黏膜模拟物生长的器官芯片微流控装置的设计、增材制造和表征。

IF 3.3 2区医学 Q2 ENGINEERING, BIOMEDICAL

Journal of the Mechanical Behavior of Biomedical Materials

Pub Date : 2024-12-19 DOI: 10.1016/j.jmbbm.2024.106877

Foteini Machla , Paraskevi Kyriaki Monou , Panagiotis Artemiou , Ioannis Angelopoulos , Vasileios Zisis , Emmanuel Panteris , Orestis Katsamenis , Eric Williams , Emmanouil Tzimtzimis , Dimitrios Tzetzis , Dimitrios Andreadis , Alexander Tsouknidas , Dimitrios Fatouros , Athina Bakopoulou

Introduction

Α customized organ-on-a-chip microfluidic device was developed for dynamic culture of oral mucosa equivalents (Oral_mucosa_chip-OMC).

Materials and methods

Additive Manufacturing (AM) was performed via stereolithography (SLA) printing. The dimensional accuracy was evaluated via microfocus computed tomography (mCT), the surface characteristics via scanning electron microscopy (SEM), while the mechanical properties via nanoindentation and compression tests. Computational fluid dynamics (CFD) optimized net forces towards the culture area. An oral mucosa equivalent comprising a multilayered epithelium derived by culture of TR146 cells at the air-liquid interface (ALI) and a lamina propria-analogue based on a collagen-I/fibrin hydrogel was maintained under ultra-precise flow conditions.

Results

An open-type device concept encompassing two interconnected chambers for long-term dynamic culture was developed and characterized for AM parameters, mechanical and biological properties. The split-inlet flow channel architecture allowed even distribution and symmetric flow velocity to the culture area. Cell viability exceeded 90%, while mCT and SEM indicated the 0° building angle as the most accurate SLA condition. CFD further showed that the 0° and 30° building angles most accurately reproduced the channel flow velocity predicted by the initial CAD model.

Conclusion

This study developed a customized, easy-to-produce, and cell-friendly OMC device, providing a 3D tool for biocompatibility assessment of biomaterials.

介绍：Α定制的器官芯片微流控装置用于口腔粘膜当量的动态培养（Oral_mucosa_chip-OMC）。材料和方法：增材制造（AM）通过立体光刻（SLA）印刷进行。通过微聚焦计算机断层扫描（mCT）评估尺寸精度，通过扫描电子显微镜（SEM）评估表面特征，通过纳米压痕和压缩测试评估力学性能。计算流体动力学（CFD）优化了朝向培养区的净力。在超精确的流动条件下，在空气-液体界面（ALI）培养TR146细胞获得的多层上皮和基于胶原- i /纤维蛋白水凝胶的固有层类似物组成的口腔黏膜等效物得以维持。结果：开发了一种开放式装置概念，包括两个相互连接的室，用于长期动态培养，并对AM参数，机械和生物特性进行了表征。分流入口流道结构允许均匀分布和对称的流速到培养区。细胞存活率超过90%，而mCT和SEM显示0°建筑角是最准确的SLA条件。CFD进一步表明，0°和30°建筑角最准确地再现了初始CAD模型预测的通道流速。结论：本研究开发了一种定制的、易于制作的、细胞友好的OMC装置，为生物材料的生物相容性评估提供了一种3D工具。

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引用次数: 0