Pub Date : 1990-09-30DOI: 10.14921/JSCC1971B.19.3_315
S. Yamashita, M. Miyashita, Koichiro Tsubokawa
Carboxyl terminal peptide bond was modified by thiohydantoin ring formation by the treatment of the C-terminal amino acid with trifluoroacetic anhydride and thiocyanate . The resulting modified peptide bond, peptidyl thiohydantoin, was then hydrolyzed by irradiating microwave (2450 MHz) in the presence of weak acid (2N HCI) for short duration (3 min). The thiohydantoin derivative of amino acid thus split was separated and identified by high performance liquid chromatography (HPLC). Microwave irradiation was found to be highly specific for the hydrolysis of the modified peptide bond and mild enough not to harm the residual peptide bonds.
{"title":"Microwave Irradiation to Hydrolyze Modified Peptide Bonds","authors":"S. Yamashita, M. Miyashita, Koichiro Tsubokawa","doi":"10.14921/JSCC1971B.19.3_315","DOIUrl":"https://doi.org/10.14921/JSCC1971B.19.3_315","url":null,"abstract":"Carboxyl terminal peptide bond was modified by thiohydantoin ring formation by the treatment of the C-terminal amino acid with trifluoroacetic anhydride and thiocyanate . The resulting modified peptide bond, peptidyl thiohydantoin, was then hydrolyzed by irradiating microwave (2450 MHz) in the presence of weak acid (2N HCI) for short duration (3 min). The thiohydantoin derivative of amino acid thus split was separated and identified by high performance liquid chromatography (HPLC). Microwave irradiation was found to be highly specific for the hydrolysis of the modified peptide bond and mild enough not to harm the residual peptide bonds.","PeriodicalId":39360,"journal":{"name":"Japanese Journal of Clinical Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1990-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66734367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1990-06-30DOI: 10.14921/JSCC1971B.19.2_103
H. Mezdour, A. Yamamoto
Atherosclerosis and thrombosis, major causes of heart attacks and strokes, are diseases of multiple and interactive aetiologies. Despite this frequently fatal association, there has been no evidence linking thrombosis to the earlier process of plaque formation. Lipoprotein(a) described by Berg in 19631), may be the first one. Indeed there is a considerable resurgence of interest in Lp(a), since there is strong evidence that the level of circulating Lp(a) represents an independent risk factor for ischemic disease with probably greater predictive potential than other lipoprotein traits2-4). Moreover, there is a remarkable structural homology between apo(a) and a portion of plasminogen molecule5). This unexpected discovery puts Lp(a) forward as a potential bridge between the fields of atherosclerosis and thrombosis6,4). Our purposal is to up-date knowledge on Lp(a) biochemistry and potential atherogenicity according to recent discoveries.
{"title":"Lp (a) Lipoprotein Biochemistry and Potential Role in Atherogenesis","authors":"H. Mezdour, A. Yamamoto","doi":"10.14921/JSCC1971B.19.2_103","DOIUrl":"https://doi.org/10.14921/JSCC1971B.19.2_103","url":null,"abstract":"Atherosclerosis and thrombosis, major causes of heart attacks and strokes, are diseases of multiple and interactive aetiologies. Despite this frequently fatal association, there has been no evidence linking thrombosis to the earlier process of plaque formation. Lipoprotein(a) described by Berg in 19631), may be the first one. Indeed there is a considerable resurgence of interest in Lp(a), since there is strong evidence that the level of circulating Lp(a) represents an independent risk factor for ischemic disease with probably greater predictive potential than other lipoprotein traits2-4). Moreover, there is a remarkable structural homology between apo(a) and a portion of plasminogen molecule5). This unexpected discovery puts Lp(a) forward as a potential bridge between the fields of atherosclerosis and thrombosis6,4). Our purposal is to up-date knowledge on Lp(a) biochemistry and potential atherogenicity according to recent discoveries.","PeriodicalId":39360,"journal":{"name":"Japanese Journal of Clinical Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1990-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66734126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1990-06-30DOI: 10.14921/JSCC1971B.19.2_124
Akira Abe, M. Okuno, A. Noma, Toru Imamine, Toshiyuki Nakamura, Y. Muto
{"title":"Enzymatic Assay of Tyrosine and Branched-Chain Amino Acids in Plasma and Its Application to Liver Diseases","authors":"Akira Abe, M. Okuno, A. Noma, Toru Imamine, Toshiyuki Nakamura, Y. Muto","doi":"10.14921/JSCC1971B.19.2_124","DOIUrl":"https://doi.org/10.14921/JSCC1971B.19.2_124","url":null,"abstract":"","PeriodicalId":39360,"journal":{"name":"Japanese Journal of Clinical Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1990-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66734246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1990-06-30DOI: 10.14921/JSCC1971B.19.2_113
A. Irie, Saori Takahashi, Y. Katayama, T. Matsuyama, Y. Shibata, Y. Miyake
{"title":"A Study on Antigenic Determinants of Human Urinary Kallikrein and Prokallikrein","authors":"A. Irie, Saori Takahashi, Y. Katayama, T. Matsuyama, Y. Shibata, Y. Miyake","doi":"10.14921/JSCC1971B.19.2_113","DOIUrl":"https://doi.org/10.14921/JSCC1971B.19.2_113","url":null,"abstract":"","PeriodicalId":39360,"journal":{"name":"Japanese Journal of Clinical Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1990-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66734182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1990-03-31DOI: 10.14921/JSCC1971B.19.1_46
Kojiro Matsumoto, H. Kubo, H. Kashiwabara, H. Yuki
{"title":"Automated Determination of Drugs in Serum by Column-Switching High-Performance Liquid Chromatography V. Separation of Cyclosporins","authors":"Kojiro Matsumoto, H. Kubo, H. Kashiwabara, H. Yuki","doi":"10.14921/JSCC1971B.19.1_46","DOIUrl":"https://doi.org/10.14921/JSCC1971B.19.1_46","url":null,"abstract":"","PeriodicalId":39360,"journal":{"name":"Japanese Journal of Clinical Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1990-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66734061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1990-03-31DOI: 10.14921/JSCC1971B.19.1_53
Y. Seyama, M. Hayashi, E. Usami, H. Tsuchida, S. Tokudome, S. Yamashita
Non-defatted portion of each lyophilized human and experimental atherosclerotic aortae were fractionated sequentially into glycosaminoglycan, glycoprotein, collagen and elastin, a modified method of directly non-dellpidemic elastin fractionation (Kramsch's method). The cholesterol contents, composition offatty acids of cholesterol ester and the lipid composition i the nondelipidated fractions were determined. The following results were observed:1) The accumulation of cholesterol in the arch, thoracic and abdominal aortic elastin fraction (fr.)accounted for 60-90% of the sum of the total cholesterol content of each connective tissue. 2) The elasin-bound fr. was digested by treatment of Pancreatic elastase, and fractionated into supernatant (sup.) and precipitate(ppt.). and then the ratio of digested cholesterol value (sup./sup. ppt.) was found to be about 70-90%.3) The intimal elastin fr.and collagen fr. from atherosclerotic plaques contained mach more cholesterol ester than other fractions. While other frs. (glycosaminoglycan fr. and glycoprotein fr.) from various layers contained free fatty acids as the most lipid constituent. 4) Characteristic feature of fatty acid moieties of cholesterol ester in the elastin fr. may be in that the ratios of C18:2, C18:1 and C18:2 are markedly changed, while C16:0 remains constant. 5) The mode of lipid deposition in the arterial elastin fr. obtained from the experimental nimals (rabbits and rats) was similar to the phenomenon of lipid accumulation i the human atherosclerotic aorta. The present method allowed estimation of lipid deposition in a small sample of aorta (about 10-20 mg of dry weight), and the cholesterol content of the connective tissue fraction may thus be used as one of the biochemical therosclerotic indices, proving the degree of severity together with histopathological methods.
{"title":"Basic Study on Non-Delipidemic Fractionation of Aortic Connective Tissue of Human and Experimental Atherosclerosis","authors":"Y. Seyama, M. Hayashi, E. Usami, H. Tsuchida, S. Tokudome, S. Yamashita","doi":"10.14921/JSCC1971B.19.1_53","DOIUrl":"https://doi.org/10.14921/JSCC1971B.19.1_53","url":null,"abstract":"Non-defatted portion of each lyophilized human and experimental atherosclerotic aortae were fractionated sequentially into glycosaminoglycan, glycoprotein, collagen and elastin, a modified method of directly non-dellpidemic elastin fractionation (Kramsch's method). The cholesterol contents, composition offatty acids of cholesterol ester and the lipid composition i the nondelipidated fractions were determined. The following results were observed:1) The accumulation of cholesterol in the arch, thoracic and abdominal aortic elastin fraction (fr.)accounted for 60-90% of the sum of the total cholesterol content of each connective tissue. 2) The elasin-bound fr. was digested by treatment of Pancreatic elastase, and fractionated into supernatant (sup.) and precipitate(ppt.). and then the ratio of digested cholesterol value (sup./sup. ppt.) was found to be about 70-90%.3) The intimal elastin fr.and collagen fr. from atherosclerotic plaques contained mach more cholesterol ester than other fractions. While other frs. (glycosaminoglycan fr. and glycoprotein fr.) from various layers contained free fatty acids as the most lipid constituent. 4) Characteristic feature of fatty acid moieties of cholesterol ester in the elastin fr. may be in that the ratios of C18:2, C18:1 and C18:2 are markedly changed, while C16:0 remains constant. 5) The mode of lipid deposition in the arterial elastin fr. obtained from the experimental nimals (rabbits and rats) was similar to the phenomenon of lipid accumulation i the human atherosclerotic aorta. The present method allowed estimation of lipid deposition in a small sample of aorta (about 10-20 mg of dry weight), and the cholesterol content of the connective tissue fraction may thus be used as one of the biochemical therosclerotic indices, proving the degree of severity together with histopathological methods.","PeriodicalId":39360,"journal":{"name":"Japanese Journal of Clinical Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1990-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66734108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1990-03-31DOI: 10.14921/JSCC1971B.19.1_31
Katsuhik Sugino, S. Kobatake, S. Matsuura
A hybridoma secreting monoclonal anti-human IgA antibody was subcloned and adapted to serum serum-free medium. The cells have stably grown and produced an antibody. The monoclonal ntibody (MoAb) thus produced in this serum-free medium was simply purified by precipitation. Characteristics of the antibody was comparable to that obtained from mouse ascitic tumor.It was also demonstrated to be utilized in an automated turbidimetric assay. MoAbs produced by serum-free Cell culture system may offer great potential for the immunodiagnostic reagents.
{"title":"An Anti-Human IgA Monoclonal Antibody Produced by Serum-Free Cell Culture","authors":"Katsuhik Sugino, S. Kobatake, S. Matsuura","doi":"10.14921/JSCC1971B.19.1_31","DOIUrl":"https://doi.org/10.14921/JSCC1971B.19.1_31","url":null,"abstract":"A hybridoma secreting monoclonal anti-human IgA antibody was subcloned and adapted to serum serum-free medium. The cells have stably grown and produced an antibody. The monoclonal ntibody (MoAb) thus produced in this serum-free medium was simply purified by precipitation. Characteristics of the antibody was comparable to that obtained from mouse ascitic tumor.It was also demonstrated to be utilized in an automated turbidimetric assay. MoAbs produced by serum-free Cell culture system may offer great potential for the immunodiagnostic reagents.","PeriodicalId":39360,"journal":{"name":"Japanese Journal of Clinical Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1990-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66733994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1990-03-31DOI: 10.14921/JSCC1971B.19.1_28
H. Ihara, Y. Aoki, T. Aoki
{"title":"Hydrolysis of Chromozyme TH by Fibrinogen Antiserum","authors":"H. Ihara, Y. Aoki, T. Aoki","doi":"10.14921/JSCC1971B.19.1_28","DOIUrl":"https://doi.org/10.14921/JSCC1971B.19.1_28","url":null,"abstract":"","PeriodicalId":39360,"journal":{"name":"Japanese Journal of Clinical Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1990-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66733945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1990-03-31DOI: 10.14921/JSCC1971B.19.1_37
S. Saheki, A. Takeda, Kazuo Miyamoto, I. Takahashi, M. Murase, N. Takeuchi
{"title":"Degradation of Apoproteins in High Density Lipoproteins with High or Low Contents of Amyloid A Proteins by Polymorphonuclear Leukocytes in Vitro","authors":"S. Saheki, A. Takeda, Kazuo Miyamoto, I. Takahashi, M. Murase, N. Takeuchi","doi":"10.14921/JSCC1971B.19.1_37","DOIUrl":"https://doi.org/10.14921/JSCC1971B.19.1_37","url":null,"abstract":"","PeriodicalId":39360,"journal":{"name":"Japanese Journal of Clinical Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1990-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66734014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1989-12-30DOI: 10.14921/JSCC1971B.18.4_172
J. Iwata, T. Suga
{"title":"Determination of 17-0xosteroid Sulfates and Glucuronides","authors":"J. Iwata, T. Suga","doi":"10.14921/JSCC1971B.18.4_172","DOIUrl":"https://doi.org/10.14921/JSCC1971B.18.4_172","url":null,"abstract":"","PeriodicalId":39360,"journal":{"name":"Japanese Journal of Clinical Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1989-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66733901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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