Pub Date : 1989-12-30DOI: 10.14921/JSCC1971B.18.4_184
J. Kino, T. Yoshida, C. Asamatsu, Yoshihisa Sato, Hiroko Cho, K. Shiba, S. Tomura, Toshihiko Hayashi
Shiseido Basic Research Laboratories, 1050 Nippa-cho, Kohoku-ku, Yokohama, 223, Japan *Department of Clinical Biochemistry , Faculty of Medicine, Tokyo Medical and Dental University, 1-5-4, Yushima, Bunkyo-ku, Tokyo, 113, Japan **Department of Internal Medicine, Nakano General Hospital, 4-59-16 Chuo, Nakano-ku, Tokyo. 164, Japan ***Department of Chemistry, College of Arts and Sciences, The University of Tokyo, 3-8-1 Komaba, Meguro-ku, Tokyo, 153, Japan
{"title":"Determination of Type IV Collgen Level in Serum with Monoclonal Antibodies and Its Application to Liver Diseases","authors":"J. Kino, T. Yoshida, C. Asamatsu, Yoshihisa Sato, Hiroko Cho, K. Shiba, S. Tomura, Toshihiko Hayashi","doi":"10.14921/JSCC1971B.18.4_184","DOIUrl":"https://doi.org/10.14921/JSCC1971B.18.4_184","url":null,"abstract":"Shiseido Basic Research Laboratories, 1050 Nippa-cho, Kohoku-ku, Yokohama, 223, Japan *Department of Clinical Biochemistry , Faculty of Medicine, Tokyo Medical and Dental University, 1-5-4, Yushima, Bunkyo-ku, Tokyo, 113, Japan **Department of Internal Medicine, Nakano General Hospital, 4-59-16 Chuo, Nakano-ku, Tokyo. 164, Japan ***Department of Chemistry, College of Arts and Sciences, The University of Tokyo, 3-8-1 Komaba, Meguro-ku, Tokyo, 153, Japan","PeriodicalId":39360,"journal":{"name":"Japanese Journal of Clinical Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1989-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66733977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1989-12-30DOI: 10.14921/JSCC1971B.18.4_191
K. Hiramatsu, S. Kamei, K. Iwasaki
A simple and sensitive high performance liquid chromatographic determination method for free polyamines and their acetylated derivatives was developed. Polyamines were separated on an octadecyl polymer column with isocratic elution. The separated polyamines were introduced into an enzyme reactor containing immobilized acylpolyamine amidohydrolase, amine oxidase (flavin-containing) and putrescine oxidase, in which they were deacetylated and oxidizld to generate hydrogen peroxide. The amount of hydrogen peroxide generated was then determined with an electrochemical detector. When a mixture of nine pure
{"title":"A New Determination Method for Polyamines and Their Acetylated Derivatives Involving High Performance Liquid Chromatography with an Enzyme Reactor and an Electrochemical Detector","authors":"K. Hiramatsu, S. Kamei, K. Iwasaki","doi":"10.14921/JSCC1971B.18.4_191","DOIUrl":"https://doi.org/10.14921/JSCC1971B.18.4_191","url":null,"abstract":"A simple and sensitive high performance liquid chromatographic determination method for free polyamines and their acetylated derivatives was developed. Polyamines were separated on an octadecyl polymer column with isocratic elution. The separated polyamines were introduced into an enzyme reactor containing immobilized acylpolyamine amidohydrolase, amine oxidase (flavin-containing) and putrescine oxidase, in which they were deacetylated and oxidizld to generate hydrogen peroxide. The amount of hydrogen peroxide generated was then determined with an electrochemical detector. When a mixture of nine pure","PeriodicalId":39360,"journal":{"name":"Japanese Journal of Clinical Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1989-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66733881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1989-12-30DOI: 10.14921/JSCC1971B.18.4_180
K. Hikida, Y. Inoue, N. Kojima, Y. Ohkura
{"title":"Determination of Pranoprofen in Serum by Automated Column-Switching High-Performance Liquid Chromatography","authors":"K. Hikida, Y. Inoue, N. Kojima, Y. Ohkura","doi":"10.14921/JSCC1971B.18.4_180","DOIUrl":"https://doi.org/10.14921/JSCC1971B.18.4_180","url":null,"abstract":"","PeriodicalId":39360,"journal":{"name":"Japanese Journal of Clinical Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1989-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66733918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1989-10-30DOI: 10.14921/JSCC1971B.18.3_95
M. Nakayama, K. Zaitsu, Y. Ohkura
In competitive enzyme-immunoassays (EIAs) of insulin, mixtures of several forms of enzyme-labeled insulins prepared using glutaraldehyde1), maleimide2) or periodate 3,4) have been used . There is no detailed comparison of enzyme-labeled insulins with respect to the sensitivity of EIA of insulin. The enzyme-labeling site (s) and the number of enzyme molecules incorporated per insulin molecule may be important factors influencing the sensitivity of the EIA. Recently we have developed a method for the preparation of horseradish peroxidase (HRP ; EC 1. 11. 1. 7) -labeled porcine insulins (HRP-insulins ; GlyA1-HRPinsulin, LysB29-HRP-insulin and GlyA1, LysB29-diHRP-insulin)5) (Fig. 1). This paper surveys the immunoreactivity of the above HRP-insulins with a mouse anti-human insulin monoclonal antibody and assesses the sensitivity of each as competitors in solid-phase EIA of human insulin. Two EIA methods for human insnlin are examined : a rapid method by simultaneous addition of HRPinsulin and a highly sensitive method by delayed addition of HRP-insulin.
{"title":"Use of Lysine (B29)-Horseradish Peroxidase Labeled Porcine Insulin in Solid Phase Antibody Enzyme-Immunoassay of Human Insulin","authors":"M. Nakayama, K. Zaitsu, Y. Ohkura","doi":"10.14921/JSCC1971B.18.3_95","DOIUrl":"https://doi.org/10.14921/JSCC1971B.18.3_95","url":null,"abstract":"In competitive enzyme-immunoassays (EIAs) of insulin, mixtures of several forms of enzyme-labeled insulins prepared using glutaraldehyde1), maleimide2) or periodate 3,4) have been used . There is no detailed comparison of enzyme-labeled insulins with respect to the sensitivity of EIA of insulin. The enzyme-labeling site (s) and the number of enzyme molecules incorporated per insulin molecule may be important factors influencing the sensitivity of the EIA. Recently we have developed a method for the preparation of horseradish peroxidase (HRP ; EC 1. 11. 1. 7) -labeled porcine insulins (HRP-insulins ; GlyA1-HRPinsulin, LysB29-HRP-insulin and GlyA1, LysB29-diHRP-insulin)5) (Fig. 1). This paper surveys the immunoreactivity of the above HRP-insulins with a mouse anti-human insulin monoclonal antibody and assesses the sensitivity of each as competitors in solid-phase EIA of human insulin. Two EIA methods for human insnlin are examined : a rapid method by simultaneous addition of HRPinsulin and a highly sensitive method by delayed addition of HRP-insulin.","PeriodicalId":39360,"journal":{"name":"Japanese Journal of Clinical Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1989-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66733693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1989-10-30DOI: 10.14921/JSCC1971B.18.3_100
T. Iwata, M. Yamaguchi, Masaru Nakamura, Y. Ohkura
{"title":"Determination of Free Cholestanol and Cholesterol in Human Serum by High-Performance Liquid Chromatography with Fluorescence Detection","authors":"T. Iwata, M. Yamaguchi, Masaru Nakamura, Y. Ohkura","doi":"10.14921/JSCC1971B.18.3_100","DOIUrl":"https://doi.org/10.14921/JSCC1971B.18.3_100","url":null,"abstract":"","PeriodicalId":39360,"journal":{"name":"Japanese Journal of Clinical Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1989-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66733669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1989-08-31DOI: 10.14921/JSCC1971B.18.2_68
R. Moran
As indicated at the outset of this report, the critical nature of the clinical requirements when blood gas testing is indicated, coupled with the labile nature of the sample and rapidly changing patient conditions makes it imperative that the analyst be aware of more than just the analysis it self Knowledge by the analyst, of the physiology of blood gas, the interpretation of basic blood gas results, the effects of the changing patient environment and the manner in which the sample is treated in a few short minutes from collection to reporting are equally important if one is to assure timely and clinically meaningful results.
{"title":"Preanalytical Considerations in Blood Gas Analysis Total Quality Issues for the Clinical Laboratory","authors":"R. Moran","doi":"10.14921/JSCC1971B.18.2_68","DOIUrl":"https://doi.org/10.14921/JSCC1971B.18.2_68","url":null,"abstract":"As indicated at the outset of this report, the critical nature of the clinical requirements when blood gas testing is indicated, coupled with the labile nature of the sample and rapidly changing patient conditions makes it imperative that the analyst be aware of more than just the analysis it self Knowledge by the analyst, of the physiology of blood gas, the interpretation of basic blood gas results, the effects of the changing patient environment and the manner in which the sample is treated in a few short minutes from collection to reporting are equally important if one is to assure timely and clinically meaningful results.","PeriodicalId":39360,"journal":{"name":"Japanese Journal of Clinical Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1989-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66733621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1989-08-31DOI: 10.14921/JSCC1971B.18.2_63
Y. Aoki, Z. Ogawa, H. Itoh
{"title":"Ketone Body Ratio in the Blood in Acute and Subacute Cholestatic Liver Injury","authors":"Y. Aoki, Z. Ogawa, H. Itoh","doi":"10.14921/JSCC1971B.18.2_63","DOIUrl":"https://doi.org/10.14921/JSCC1971B.18.2_63","url":null,"abstract":"","PeriodicalId":39360,"journal":{"name":"Japanese Journal of Clinical Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1989-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66733613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1989-08-31DOI: 10.14921/JSCC1971B.18.2_35
Y. Aoki, K. Mitsuta, M. Kohno, H. Itoh
{"title":"Electron Spin Resonance Studies on Microsomal Membrane Fluidity in Cholestatic Liver Injury Induced with α-Naphthylisothiocyanate","authors":"Y. Aoki, K. Mitsuta, M. Kohno, H. Itoh","doi":"10.14921/JSCC1971B.18.2_35","DOIUrl":"https://doi.org/10.14921/JSCC1971B.18.2_35","url":null,"abstract":"","PeriodicalId":39360,"journal":{"name":"Japanese Journal of Clinical Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1989-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66733567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1989-06-30DOI: 10.14921/JSCC1971B.18.1_17
M. Oimomi, Y. Maeda, F. Hata, T. Nakamichi, S. Baba, T. Iga, Misao Yamamoto
The levels of advanced-stage products of the Maillard reaction in lens proteins are considered to reflect the sum of accumulated glycation over time, because there is little turnover of lens crystallins. Thus, we investigated the relationship between the degree of diabetic complications, i. e. diabetic retinopathy and levels of advanced-stage products accumulated in the lens proteins prior to the surgical resection. The level of advanced-stage products in the lens proteins increased with the progression of diabetic retinopathy. The differences among the level in the groups of each diabetic retinopathy were significant. These results suggest that glycation measured by advanced Maillard product in the lens protein plays an important role in the etiology of diabetic complications.
{"title":"Glycation and the Development of Diabetic Retinopathy","authors":"M. Oimomi, Y. Maeda, F. Hata, T. Nakamichi, S. Baba, T. Iga, Misao Yamamoto","doi":"10.14921/JSCC1971B.18.1_17","DOIUrl":"https://doi.org/10.14921/JSCC1971B.18.1_17","url":null,"abstract":"The levels of advanced-stage products of the Maillard reaction in lens proteins are considered to reflect the sum of accumulated glycation over time, because there is little turnover of lens crystallins. Thus, we investigated the relationship between the degree of diabetic complications, i. e. diabetic retinopathy and levels of advanced-stage products accumulated in the lens proteins prior to the surgical resection. The level of advanced-stage products in the lens proteins increased with the progression of diabetic retinopathy. The differences among the level in the groups of each diabetic retinopathy were significant. These results suggest that glycation measured by advanced Maillard product in the lens protein plays an important role in the etiology of diabetic complications.","PeriodicalId":39360,"journal":{"name":"Japanese Journal of Clinical Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1989-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66733538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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