Summary The pathogenicity of indigenous isolates of Beauveria bassiana, Metarhizium anisopliae and Isaria fumosorosea was evaluated in the laboratory against larvae and adults of the sisal weevil, Scyphophorus acupunctatus. Inoculation was achieved via immersion of individuals into conidia suspensions of different concentrations. All three fungal species proved high pathogenicity against larvae of the weevil, causing 100% mortality in most of the treatments. Beauveria bassiana caused the highest mortality of the adults (86.67±12%), followed by M. anisopliae (46.67±17.8%) and I. fumosorosea (40±17.5%). Mean survival time also differed significantly among treatments and life stages of the weevil. In total, larvae survived significantly fewer days than adults post infection. Results of the present study indicate the potential of indigenous strains of entomopathogenic fungi as biological control agents against the invasive weevil.
{"title":"Pathogenicity of indigenous strains of three entomopathogenic fungi to the sisal weevil, Scyphophorus acupunctatus (Gyllenhal) (Coleoptera: Curculionidae)","authors":"V. Gkounti, D. Markoyiannaki, D. Kontodimas","doi":"10.1515/hppj-2015-0007","DOIUrl":"https://doi.org/10.1515/hppj-2015-0007","url":null,"abstract":"Summary The pathogenicity of indigenous isolates of Beauveria bassiana, Metarhizium anisopliae and Isaria fumosorosea was evaluated in the laboratory against larvae and adults of the sisal weevil, Scyphophorus acupunctatus. Inoculation was achieved via immersion of individuals into conidia suspensions of different concentrations. All three fungal species proved high pathogenicity against larvae of the weevil, causing 100% mortality in most of the treatments. Beauveria bassiana caused the highest mortality of the adults (86.67±12%), followed by M. anisopliae (46.67±17.8%) and I. fumosorosea (40±17.5%). Mean survival time also differed significantly among treatments and life stages of the weevil. In total, larvae survived significantly fewer days than adults post infection. Results of the present study indicate the potential of indigenous strains of entomopathogenic fungi as biological control agents against the invasive weevil.","PeriodicalId":39459,"journal":{"name":"Hellenic Plant Protection Journal","volume":"8 1","pages":"46 - 54"},"PeriodicalIF":0.0,"publicationDate":"2015-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/hppj-2015-0007","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66854546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Summary The impact of the monomolecular surface fi lm AquatainTM against mosquito larvae was tested in a rice field in Central Greece. AquatainTM was poured in a 1.2 hectare rice paddy along the one side of the field. Laboratory reared mosquito larvae of Culex pipiens were introduced into cages placed in three different transects along the short side of the rice paddy. Larval mortality was counted 3, 6, 15 and 25 days after application. In the line located closer to the site of AquatainTM application, larval mortality ranged from 100% to 70%, 25 days after application. Mortality ranged from 88% to 25% in the middle transect and from 42%, to 10% in the more distant line. The results indicated that AquatainTM provides sufficient larval control in wide mosquito larval habitats
{"title":"Evaluation of AquatainTM monomolecular surface film against mosquito larvae of Culex pipiens in a full-grown rice field in Greece","authors":"I. Kioulos, G. Koliopoulos","doi":"10.1515/hppj-2015-0004","DOIUrl":"https://doi.org/10.1515/hppj-2015-0004","url":null,"abstract":"Summary The impact of the monomolecular surface fi lm AquatainTM against mosquito larvae was tested in a rice field in Central Greece. AquatainTM was poured in a 1.2 hectare rice paddy along the one side of the field. Laboratory reared mosquito larvae of Culex pipiens were introduced into cages placed in three different transects along the short side of the rice paddy. Larval mortality was counted 3, 6, 15 and 25 days after application. In the line located closer to the site of AquatainTM application, larval mortality ranged from 100% to 70%, 25 days after application. Mortality ranged from 88% to 25% in the middle transect and from 42%, to 10% in the more distant line. The results indicated that AquatainTM provides sufficient larval control in wide mosquito larval habitats","PeriodicalId":39459,"journal":{"name":"Hellenic Plant Protection Journal","volume":"8 1","pages":"21 - 26"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66854529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Summary Two invasive mealybug species, Phenacoccus peruvianus Granara de Willink and Phenacoccus madeirensis Green (Hemiptera: Pseudococcidae), have been recorded on new species of ornamental plants in diff erent regions of Greece. Phenacoccus peruvianus was recorded in Athens on Cestrum nocturnum L. (Solanaceae) in September 2013. Phenacoccus madeirensis was found in Kalamata (Peloponnese) on Aloysia citriodora Palau (Verbenaceae) in May 2014 and on Osteospermum jucundum (Phillips) (Asteraceae) in July 2014. This is the first record of O. jucundum as host plant of P. madeirensis
{"title":"Record of Phenacoccus peruvianus Granara de Willing and Phenacoccus madeirensis (Hemiptera: Pseudococcidae) on new host ornamental plants in Greece","authors":"G. Stathas, E. Kartsonas, A. Darras","doi":"10.1515/hppj-2015-0002","DOIUrl":"https://doi.org/10.1515/hppj-2015-0002","url":null,"abstract":"Summary Two invasive mealybug species, Phenacoccus peruvianus Granara de Willink and Phenacoccus madeirensis Green (Hemiptera: Pseudococcidae), have been recorded on new species of ornamental plants in diff erent regions of Greece. Phenacoccus peruvianus was recorded in Athens on Cestrum nocturnum L. (Solanaceae) in September 2013. Phenacoccus madeirensis was found in Kalamata (Peloponnese) on Aloysia citriodora Palau (Verbenaceae) in May 2014 and on Osteospermum jucundum (Phillips) (Asteraceae) in July 2014. This is the first record of O. jucundum as host plant of P. madeirensis","PeriodicalId":39459,"journal":{"name":"Hellenic Plant Protection Journal","volume":"8 1","pages":"12 - 14"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/hppj-2015-0002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66854482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Summary Euphorbia heterophylla (wild poinsettia) is reported as an emerging weed in cotton and processing tomato in Kopaida plain, region of Viotia, in central Greece. This is the first record of E. heterophylla in tomato crop in Greece. In a field experiment, mature plants grown under weed-free conditions produced on average 19 heads, 64 capsules, and 192 seeds per individual plant. Mature seeds exhibited no dormancy and the maximum germination (82 to 90%) occurred at temperatures from 25 to 35°C, with a drastic decline (<38%) at 15 and 40°C. Light had no significant effect on seed germination in the whole range of temperatures tested. Fully mature plants were taller than cotton, exerting strong competition. Having no light dependence for germination, seeds might have the potential to germinate and emerge from greater soil depths. This short communication summarizes information for the identification, seed germination and growth of this weed species that would support a proper weed management
{"title":"Wild poinsettia (Euphorbia heterophylla): an emerging weed in cotton and processing tomato in Greece","authors":"D. Chachalis","doi":"10.1515/hppj-2015-0005","DOIUrl":"https://doi.org/10.1515/hppj-2015-0005","url":null,"abstract":"Summary Euphorbia heterophylla (wild poinsettia) is reported as an emerging weed in cotton and processing tomato in Kopaida plain, region of Viotia, in central Greece. This is the first record of E. heterophylla in tomato crop in Greece. In a field experiment, mature plants grown under weed-free conditions produced on average 19 heads, 64 capsules, and 192 seeds per individual plant. Mature seeds exhibited no dormancy and the maximum germination (82 to 90%) occurred at temperatures from 25 to 35°C, with a drastic decline (<38%) at 15 and 40°C. Light had no significant effect on seed germination in the whole range of temperatures tested. Fully mature plants were taller than cotton, exerting strong competition. Having no light dependence for germination, seeds might have the potential to germinate and emerge from greater soil depths. This short communication summarizes information for the identification, seed germination and growth of this weed species that would support a proper weed management","PeriodicalId":39459,"journal":{"name":"Hellenic Plant Protection Journal","volume":"8 1","pages":"27 - 32"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/hppj-2015-0005","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66854534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Summary Common burdock (Arctium minus) is a common biennial weed of non-arable land in typical rural settings of Orestiada, Greece. The aim of this study was to describe the basic morphological traits of this species throughout the main phenological stages of its life cycle and to obtain some insight into its growth and productivity in Orestiada. Based on our observations, the plants occurred most commonly in moist and fertile soils, usually as isolated individuals or in small patches near the parent plants. The species is characterized by its large basal ‘elephant-ear’ leaves during the vegetative stage, appearing in alternate arrangement, with irregularly wavy and non-toothed edges, as well as with long hollow stalks forming a noticeable furrow on the top. By monitoring individual plants, it was found that fl owering (in the second year of growth) mostly occurred from late June up to early August. The fl owers were purple, occurring in bristly heads at the top of the stem. The bristly heads formed a fruit, containing small black seeds. The average number of capitula per plant, from randomly selected populations in Orestiada, was found to be 69.7 and 57.7 respectively, whereas the mean seed number per capitulum reached 30.3 and 33.3 seeds, respectively
{"title":"Common burdock (Arctium minus): a common weed of nonarable land in Orestiada, Greece","authors":"C. Damalas, C. Alexoudis, S. Koutroubas","doi":"10.1515/hppj-2015-0003","DOIUrl":"https://doi.org/10.1515/hppj-2015-0003","url":null,"abstract":"Summary Common burdock (Arctium minus) is a common biennial weed of non-arable land in typical rural settings of Orestiada, Greece. The aim of this study was to describe the basic morphological traits of this species throughout the main phenological stages of its life cycle and to obtain some insight into its growth and productivity in Orestiada. Based on our observations, the plants occurred most commonly in moist and fertile soils, usually as isolated individuals or in small patches near the parent plants. The species is characterized by its large basal ‘elephant-ear’ leaves during the vegetative stage, appearing in alternate arrangement, with irregularly wavy and non-toothed edges, as well as with long hollow stalks forming a noticeable furrow on the top. By monitoring individual plants, it was found that fl owering (in the second year of growth) mostly occurred from late June up to early August. The fl owers were purple, occurring in bristly heads at the top of the stem. The bristly heads formed a fruit, containing small black seeds. The average number of capitula per plant, from randomly selected populations in Orestiada, was found to be 69.7 and 57.7 respectively, whereas the mean seed number per capitulum reached 30.3 and 33.3 seeds, respectively","PeriodicalId":39459,"journal":{"name":"Hellenic Plant Protection Journal","volume":"8 1","pages":"15 - 20"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66854524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Eslami, S. A. Khodaparast, S. Mousanejad, F. Padasht Dehkaei
Summary Sclerotium rolfsii is a soil borne pathogen responsible for root and stem rot on a wide range of crops. This study was conducted to identify the virulence of different S. rolfsii isolates on a susceptible local peanut germplasm and determine the resistance of 20 peanut genotypes to the most virulent isolate and also the relationship between virulence and mycelial compatibility groups (MCGs). Seventy eight isolates of this fungus from 10 host plants and six known MCGs were used in the experiment. The experiment was done in greenhouse conditions (25±5°C) using a complete randomized block design with three replications. Pots containing sterile soil (pH=6.7) were inoculated with barley seeds colonized by each isolate separately before being seeded with the peanut germplasm. Disease severity was assessed by scoring the wilting, yellowing or death of plants, mycelia or sclerotia production on the soil surface or on plant stem, stem area affected (%) and stem lesion length, at the stage of plant maturity. Also, shoot wet weight and plant height were recorded at this stage. According to the results of the pathogenicity tests, all of the isolates were virulent on the susceptible peanut germplasm and the virulence diff ered signifi cantly between the isolates (P≤0.01). There was no relationship between the virulence of the five groups of isolates identified in the present study and the MCGs. The peanut genotype 140, which was better than the others based on seed size, plant height and the canopy size, was also the most resistant one
{"title":"Evaluation of the virulence of Sclerotium rolfsii isolates on Arachis hypogaea and screening for resistant genotypes in greenhouse conditions","authors":"A. Eslami, S. A. Khodaparast, S. Mousanejad, F. Padasht Dehkaei","doi":"10.1515/hppj-2015-0001","DOIUrl":"https://doi.org/10.1515/hppj-2015-0001","url":null,"abstract":"Summary Sclerotium rolfsii is a soil borne pathogen responsible for root and stem rot on a wide range of crops. This study was conducted to identify the virulence of different S. rolfsii isolates on a susceptible local peanut germplasm and determine the resistance of 20 peanut genotypes to the most virulent isolate and also the relationship between virulence and mycelial compatibility groups (MCGs). Seventy eight isolates of this fungus from 10 host plants and six known MCGs were used in the experiment. The experiment was done in greenhouse conditions (25±5°C) using a complete randomized block design with three replications. Pots containing sterile soil (pH=6.7) were inoculated with barley seeds colonized by each isolate separately before being seeded with the peanut germplasm. Disease severity was assessed by scoring the wilting, yellowing or death of plants, mycelia or sclerotia production on the soil surface or on plant stem, stem area affected (%) and stem lesion length, at the stage of plant maturity. Also, shoot wet weight and plant height were recorded at this stage. According to the results of the pathogenicity tests, all of the isolates were virulent on the susceptible peanut germplasm and the virulence diff ered signifi cantly between the isolates (P≤0.01). There was no relationship between the virulence of the five groups of isolates identified in the present study and the MCGs. The peanut genotype 140, which was better than the others based on seed size, plant height and the canopy size, was also the most resistant one","PeriodicalId":39459,"journal":{"name":"Hellenic Plant Protection Journal","volume":"8 1","pages":"1 - 11"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/hppj-2015-0001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66854475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Summary The degradation kinetics and sorption characteristics of atrazine and metolachlor in Japanese andisol soil were evaluated using laboratory incubation of soil samples. The water content of the soil was set to field capacity while three different temperatures (5, 25 and 35°C) were considered for the experiment. First order model fitted the degradation kinetics of both herbicides under the investigated temperature range with half-lives ranging from 19.2 to 46.9 days for atrazine and from 23.4 to 66.9 days for metolachlor, respectively. The activation energies (Ea) of atrazine and metolachlor calculated using Arhenius equation were 21.47 and 23.91 kJ mol−1, respectively. The soil sorption study was conducted using the batch equilibrium process. The adsorption behaviors of atrazine and metolachlor were investigated using linear, Freundlich and Langmuir isotherms although the linear and Freundlich isotherms gave relatively high correlation coefficient (R2) and very low standard error of estimate (SEE). The free energy (ΔG°) values were in the range −30.6 to −32.0 kJ/mol, and −32.1 to −41.5 kJ/mol for atrazine and metolachlor, respectively. Thermodynamic parameters indicated that the adsorption is spontaneous, endothermic accompanied by increase in entropy. The understanding of atrazine and metolachlor sorption processes is essential to determine the pesticide fate and availability in soil for pest control, biodegradation, runoff and leaching.
{"title":"Aspect of the degradation and adsorption kinetics of atrazine and metolachlor in andisol soil","authors":"P. Jaikaew, F. Malhat, J. Boulange, H. Watanabe","doi":"10.1515/hppj-2017-0001","DOIUrl":"https://doi.org/10.1515/hppj-2017-0001","url":null,"abstract":"Summary The degradation kinetics and sorption characteristics of atrazine and metolachlor in Japanese andisol soil were evaluated using laboratory incubation of soil samples. The water content of the soil was set to field capacity while three different temperatures (5, 25 and 35°C) were considered for the experiment. First order model fitted the degradation kinetics of both herbicides under the investigated temperature range with half-lives ranging from 19.2 to 46.9 days for atrazine and from 23.4 to 66.9 days for metolachlor, respectively. The activation energies (Ea) of atrazine and metolachlor calculated using Arhenius equation were 21.47 and 23.91 kJ mol−1, respectively. The soil sorption study was conducted using the batch equilibrium process. The adsorption behaviors of atrazine and metolachlor were investigated using linear, Freundlich and Langmuir isotherms although the linear and Freundlich isotherms gave relatively high correlation coefficient (R2) and very low standard error of estimate (SEE). The free energy (ΔG°) values were in the range −30.6 to −32.0 kJ/mol, and −32.1 to −41.5 kJ/mol for atrazine and metolachlor, respectively. Thermodynamic parameters indicated that the adsorption is spontaneous, endothermic accompanied by increase in entropy. The understanding of atrazine and metolachlor sorption processes is essential to determine the pesticide fate and availability in soil for pest control, biodegradation, runoff and leaching.","PeriodicalId":39459,"journal":{"name":"Hellenic Plant Protection Journal","volume":"10 1","pages":"1 - 14"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/hppj-2017-0001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66854715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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