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Molecular advances on agricultural crop improvement to meet current cultivating demands 农业作物改良的分子进展以满足当前的种植需求
Q3 Agricultural and Biological Sciences Pub Date : 2019-07-01 DOI: 10.2478/HPPJ-2019-0006
T. Margaritopoulou, D. Milioni
Sunflower, maize and potato are among the world’s principal crops. In order to improve various traits, these crops have been genetically engineered to a great extent. Even though molecular markers for simple traits such as, fertility, herbicide tolerance or specific pathogen resistance have been successfully used in marker-assisted breeding programs for years, agronomical important complex quantitative traits like yield, biotic and abiotic stress resistance and seed quality content are challenging and require whole genome approaches. Collections of genetic resources for these crops are conserved worldwide and represent valuable resources to study complex traits. Nowadays technological advances and the availability of genome sequence have made novel approaches on the whole genome level possible. Molecular breeding, including both transgenic approach and marker-assisted breeding have facilitated the production of large amounts of markers for high density maps and allowed genome-wide association studies and genomic selection in sunflower, maize and potato. Marker-assisted selection related to hybrid performance has shown that genomic selection is a successful approach to address complex quantitative traits and to facilitate speeding up breeding programs in these crops in the future.
向日葵、玉米和土豆是世界上主要的农作物。为了改善各种性状,这些作物已经在很大程度上进行了基因工程改造。尽管简单性状的分子标记,如肥力、除草剂耐受性或特定病原体抗性已经成功地应用于标记辅助育种计划多年,但农学上重要的复杂数量性状,如产量、生物和非生物抗逆性和种子质量含量,具有挑战性,需要全基因组方法。这些作物的遗传资源在世界范围内保存,是研究复杂性状的宝贵资源。如今,技术的进步和基因组序列的可用性使全基因组水平的新方法成为可能。分子育种,包括转基因方法和标记辅助育种,促进了高密度图谱中大量标记的产生,并使向日葵、玉米和马铃薯的全基因组关联研究和基因组选择成为可能。与杂交性能相关的标记辅助选择表明,基因组选择是解决复杂数量性状和促进未来这些作物育种计划的成功方法。
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引用次数: 0
Multistrain versus single-strain plant growth promoting microbial inoculants - The compatibility issue 促进微生物接种剂的多株与单株植物生长-相容性问题
Q3 Agricultural and Biological Sciences Pub Date : 2019-07-01 DOI: 10.2478/HPPJ-2019-0007
E.-E. Thomloudi, P. Tsalgatidou, D. Douka, T.-N. Spantidos, M. Dimou, A. Venieraki, P. Katinakis
Plant Growth Promoting Microorganisms or Plant Probiotics (PGPMs) constitute a promising solution for agricultural sustainability. The concept that inoculation of PGPM mixtures may perform better in enhancing agricultural production than single strain application dates back to the discovery of plant growth rhizobacteria (PGPR) and is gaining ground in our days. This shift is highlighted by the increasing number of research publications dealing with the positive impact of microbial mixtures in promoting plant growth, controlling plant pathogens, as well as providing abiotic stress tolerance. The continuous deposition of patents as well as commercially available formulations concerning bioprotective and/or biostimulant multistrain mixtures also underlines this shift. A major issue in engineering an effective and consistent synthetic multistrain mixture appears to be the compatibility of its components. The present review provides a thorough literature survey supporting the view that treatment of plants with compatible multistrain mixtures generally exerts a better effect in plant growth and health than single-strain inoculation. Our study focuses on multistrain mixtures based on Pseudomonas, Bacillus and beneficial fungal strains, while commercial products are also being referred.
植物生长促进微生物或植物益生菌(PGPM)是农业可持续发展的一个有前景的解决方案。接种PGPM混合物可能比单一菌株应用在提高农业生产方面表现更好,这一概念可以追溯到植物生长根际细菌(PGPR)的发现,并在我们的时代得到了推广。越来越多的研究出版物强调了这一转变,这些出版物涉及微生物混合物在促进植物生长、控制植物病原体以及提供非生物胁迫耐受性方面的积极影响。关于生物保护剂和/或生物刺激剂-多雨水混合物的专利以及市售制剂的不断沉积也突显了这一转变。在工程中,一个有效且一致的合成多雨水混合物的主要问题似乎是其成分的兼容性。本综述提供了一项全面的文献调查,支持用相容的多菌株混合物处理植物通常比单菌株接种对植物生长和健康产生更好的效果。我们的研究重点是基于假单胞菌、芽孢杆菌和有益真菌菌株的多菌混合物,同时也参考了商业产品。
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引用次数: 37
First report of Fusarium proliferatum causing stem and root rot on lucky bamboo (Dracaena braunii) in Iraq 镰刀菌繁殖导致幸运竹(Dracaena braunii)茎根腐烂在伊拉克的首次报道
Q3 Agricultural and Biological Sciences Pub Date : 2019-01-01 DOI: 10.2478/hppj-2019-0001
A. Lahuf
Summary Lucky bamboo (Dracaena braunii) is a popular ornamental plant in Iraq. Individuals of this plant showing stem and root rot symptoms were observed during a survey conducted from November 2015 to February 2016 in several nurseries in Kerbala province, Iraq. Based on morphological characteristics and sequence analyses of the internal transcribed spacer (ITS) region of the ribosomal DNA (rDNA), the pathogen was identified as Fusarium proliferatum. This is the first report of stem and root rot caused by F. proliferatum on lucky bamboo (D. braunii) in Iraq.
幸运竹(Dracaena braunii)是伊拉克一种受欢迎的观赏植物。2015年11月至2016年2月,在伊拉克克尔巴拉省的几个苗圃进行的一项调查中,观察到这种植物出现茎和根腐烂症状。根据核糖体DNA(rDNA)内部转录间隔区(ITS)的形态学特征和序列分析,确定该病原体为增殖镰刀菌。这是伊拉克首次报道福竹(D.braunii)上由F.proliferum引起的茎根腐烂。
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引用次数: 8
First record of Chymomyza procnemoides (Wheeler) (Diptera: Drosophilidae) for the Turkish fauna 标题土耳其区系首次记录的拟蝇(双翅目:果蝇科)
Q3 Agricultural and Biological Sciences Pub Date : 2019-01-01 DOI: 10.2478/hppj-2019-0004
E. Zengin
Summary This is the first record of Chymomyza procnemoides (Wheeler, 1952) (Diptera: Drosophilidae) in Turkey. The specimens were obtained from bottle bait traps on apple, pear and plum fruit trees at the Uşak province in 2017.
这是土耳其首次记录到的Chymomyza procnemoides (Wheeler, 1952)(双翅目:果蝇科)。该标本于2017年在云南省苹果树、梨树和李树上用瓶饵诱捕器捕获。
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引用次数: 0
Resistance of ten common medicinal plants to the root-knot nematode Meloidogyne javanica 十种常用药用植物对爪哇根结线虫的抗性研究
Q3 Agricultural and Biological Sciences Pub Date : 2019-01-01 DOI: 10.2478/hppj-2019-0002
S. Ansari, H. Charehgani, R. Ghaderi
Summary A preliminary survey indicated that the root-knot nematode Meloidogyne javanica is widely distributed in the rhizosphere of medicinal plants in Boyer-Ahmad region (Iran). Host suitability of ten species of medicinal plants to M. javanica was examined in a pot experiment under controlled greenhouse conditions: alkakengy (Physalis alkekengi L.), chamomile (Matricaria chamomilla L.), English plantain (Plantago lanceolata L.), fennel (Foeniculum vulgare Mill.), garden anchusa (Anchusa italica Retz.), horehound (Marrubium vulgare L.), lovage (Levisticum officinale L.), sorrel (Rumex acetosella L.), thistle (Echinops adenocaulos Boiss.) and woundwort (Stachys pilifera Benth.). According to the scheme of Canto-Saenz, seven species, namely garden anchusa, fennel, horehound, alkakengy, English plantain, woundwort and sorrel can be considered susceptible hosts with gall index (GI) > 2 and reproduction factor (RF) > 1, and thistle, lovage and chamomile, can be considered as hyper-susceptible with GI > 2 and RF ≤ 1.
初步调查表明,根结线虫javanica在伊朗Boyer-Ahmad地区药用植物根际广泛分布。在温室控制条件下,通过盆栽试验研究了10种药用植物对爪哇栗鼠的寄主适宜性。碱能植物(Physalis alkekengi L.)、洋甘菊(Matricaria chamomilla L.)、英车前草(Plantago lanceolata L.)、茴香(Foeniculum vulgare Mill.)、花楸(anchusa italica Retz.)、苦楝(Marrubium vulgare L.)、丁香(Levisticum officinale L.)、酢浆草(Rumex acetosella L.)、蓟(Echinops adenocaulos Boiss.)和苦艾(Stachys pilifera Benth.)。根据Canto-Saenz的方案,花楸、茴香、苦楝、碱能、车前草、苦苣、酢膏草等7种可视为敏感寄主,其瘿指数(GI)为>,繁殖因子(RF)为>,蓟、lovage和洋甘菊为超敏感寄主,其GI >, RF≤1。
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引用次数: 3
Biodiversity and population fluctuations of parasitoids of the white peach scale, Pseudaulacaspis pentagona (Targioni-Tozzetti) (Hemiptera: Diaspididae), in kiwifruit orchards in Northern Iran 伊朗北部猕猴桃园白桃蚧拟寄生蜂的生物多样性及种群消长
Q3 Agricultural and Biological Sciences Pub Date : 2019-01-01 DOI: 10.2478/hppj-2019-0003
A. Toorani, H. Abbasipour, L. Dehghan-Dehnavi
Summary The white peach scale, Pseudaulacaspis pentagona Targioni-Tozzetti (Hemiptera: Diaspididae), is one of the most important and destructive polyphagous pests of the Rosaceae family trees. Population fluctuations and biodiversity of the hymenopteran parasitoid species associated with the pest were studied in six kiwi orchards in Iran, during one-year period. Parasitoid species abundance, species diversity indices and evenness indices were calculated. Most of the parasitoid species were dominant or eudominant. Based on the alpha diversity indices, the Najarkola region had high diversity and the Kharatkola region had low diversity. The Paeendasteh region (based on Simpson’s Diversity on Camargo evenness indices) and the Samnakola region (based on the modified Nee, and on Smith and Wilson evenness indices) were less uniform. Among the recorded parasitoids, Encarsia berlesei Howard (Hymenoptera: Aphelinidae), followed by Aphytis proclia Walker (Hymenoptera: Aphelinidae), had the highest population in all orchards.
白桃蚧(Pseudaulacaspis pentagona Targioni-Tozzetti)是桃科最重要的破坏性多食性害虫之一。在为期一年的时间里,在伊朗的六个猕猴桃果园研究了与这种害虫有关的膜翅类寄生蜂的种群波动和生物多样性。计算拟寄生物物种丰度、物种多样性指数和均匀度指数。拟寄生物种类多数为优势种或真优势种。从α多样性指数来看,纳jarkola地区多样性较高,Kharatkola地区多样性较低。Paeendasteh地区(基于Camargo均匀度指数的Simpson 's Diversity)和Samnakola地区(基于修正的Nee,以及Smith和Wilson均匀度指数)的均匀性较差。各果园寄生蜂的数量以柏氏小蜂(膜翅目:蚜蜂科)最多,其次为原蜂(膜翅目:蚜蜂科)。
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引用次数: 1
Investigating the in vitro and in vivo nematicidal performance of structurally related macrolides against the root-knot nematode, Meloidogyne incognita 研究结构相关大环内酯类对根结线虫(Meloidogyne incognita)的体内外杀线虫性能
Q3 Agricultural and Biological Sciences Pub Date : 2019-01-01 DOI: 10.2478/hppj-2019-0005
M. A. Radwan, A. Saad, H. Mesbah, Hassan Al-haj Ibrahim, M. Khalil
Summary Avermectins and spinosyns are structurally related natural products of microbial origin and belong to a new family of macrolides which are active against a vast array of invertebrate pests. In the present study, the effects of four members of macrolides; abamectin (ABM), emamectin benzoate (EMB), spinosad (SPI) and spinetoram (SPIT), on Meloidogyne incognita were investigated under in vitro and in vivo conditions. All compounds reduced egg hatching and led to high mortality of the nematode second-stage juveniles (J2). ABM showed the maximum rate of egg hatching inhibition and J2 mortality while SPIT recorded the minimum. All treatments reduced the number of galls, egg masses, eggs/egg mass in roots and J2 in the soil when compared to the control. Based on the 10 folds of the 24 h-LC50 values of J2 mortality in vitro, EMB and ABM exhibited higher percent reduction in galls (79.68 and 71.45%), egg masses (75.19 and 70.54%), eggs/egg mass (60.49 and 40.91%) and J2 in the soil (90.31 and 86.54%), respectively, compared to SPI and SPIT. Significant increase in tomato shoot height occurred in all biopesticides (10 folds) and SPIT (20 folds). SPI at 10 folds of the 24 h-LC50 values of J2 mortality in vitro, significantly increased root length while ABM at 50 folds and SPIT at 20 folds decreased root length by 5.15% and 5.88%, respectively, compared to the untreated inoculated plants. In all treatments, the dry shoot and root weights increased, compared to the untreated control. Our findings suggest that these macrolides have the ability to regulate nematode population densities and may be an alternative to classical nematicides.
阿维菌素和spinosyns是微生物来源的结构相关的天然产物,属于一个新的大环内酯类,它们对大量无脊椎动物害虫有活性。在本研究中,大环内酯的四个成员的影响;在体外和体内条件下,研究了阿维菌素(ABM)、甲维菌素苯甲酸酯(EMB)、spinosad (SPI)和spinetoram (SPIT)对无头丝虫病的作用。所有化合物都减少了卵的孵化,导致线虫第二阶段幼虫的高死亡率(J2)。ABM的孵化抑制率和J2死亡率最高,而SPIT的孵化抑制率最低。与对照相比,所有处理均减少了虫瘿数、虫卵团数、根中虫卵/虫卵团数和土壤中J2的数量。体外J2死亡率为24 h-LC50值的10倍,与SPI和SPIT相比,EMB和ABM对虫瘿(79.68和71.45%)、卵质量(75.19和70.54%)、卵/卵质量(60.49和40.91%)和土壤J2(90.31和86.54%)的降低率更高。所有生物农药(10倍)和唾液(20倍)均显著提高了番茄茎高。SPI为J2离体死亡24 h-LC50值的10倍时,根长显著增加,ABM为50倍,SPIT为20倍时,根长分别比未接种植株减少5.15%和5.88%。在所有处理中,与未处理对照相比,干梢和根重均增加。我们的研究结果表明,这些大环内酯类具有调节线虫种群密度的能力,可能是传统杀线虫剂的替代品。
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引用次数: 8
Lethal and sublethal effects of ten insecticides, used in date palm production in Saudi Arabia, on the parasitoid Trichogramma cacoeciae 沙特阿拉伯椰枣生产中使用的十种杀虫剂对寄生赤眼蜂的致死和亚致死作用
Q3 Agricultural and Biological Sciences Pub Date : 2018-07-01 DOI: 10.2478/hppj-2018-0009
M. J. Hajjar, M. Al-Masoud
Abstract Lethal and sublethal effects of ten insecticides commonly used in date palm production in Saudi Arabia were assessed in the laboratory against adults of Trichogramma cacoeciae, an important egg parasitoid of the dried fruit moth Ephestia calidella. Bioassays were conducted according to the standard protocol of the International Organization for Biological Control IOBC/WPRS/Working Group ‘Pesticides and Beneficial Organisms’. Our results showed that cypermethrin, deltamethrin, malathion, phenthoate, methomyl, and carbosulfan were moderately harmful (IOBC Class 3) to the parasitoid. The botanical insecticides azadirachtin and matrine were moderately harmful (IOBC Class 3) and slightly harmful (IOBC Class 2), respectively. The insect growth regulator pyriproxyfen was slightly harmful, whereas bistrifluron was harmless (IOBC Class 1). Regarding sublethal effects, the parasitism ratios compared to control were reduced by pyriproxyfen and azadirachtin to 49.0% and 58.0%, respectively; hence they are classified as slightly harmful insecticides (IOBC Class 2). Bistrifluron and matrine were harmless (IOBC Class 1) as parasitism ratios were reduced by 9.2% and 27.6%, respectively. Longevity of adults exposed to bistrifluron and matrine (3.6 and 3.3 days, respectively) and to pyriproxyfen and azadirachtin (1.7 and 1.3 days, respectively) was significantly lower than that in control (4.67 days). In semi-field tests, residues of most insecticides on leaves of tomato, a common host plant of lepidopteran pests parasitized by T. cacoeciae, were considered moderately harmful to harmful based on parasitoid mortality at 24 h post-treatment whereas they were slightly harmful at 7 and 14 days post-treatment
摘要在实验室中,对沙特阿拉伯椰枣生产中常用的10种杀虫剂对干燥果蛾(Ephesia calidella)的重要卵寄生赤眼蜂(Trichogramma cacoeciae)成虫的致死和亚致死效果进行了评估。生物测定是根据国际生物控制组织IOBC/WPRS/“农药和有益生物”工作组的标准议定书进行的。结果表明,氯氰菊酯、溴氰菊酯、马拉硫磷、乐果、灭多威和碳硫丹对该类寄生虫的危害为中度(IOBC 3级)。植物杀虫剂印楝素和苦参碱的危害分别为中度(IOBC 3级)和轻度(IOBC 2级)。昆虫生长调节剂吡嘧磺芬危害轻微,而双三氟隆无害(IOBC 1级)。关于亚致死效应,与对照相比,吡嘧磺芬和印楝素的寄生率分别降低到49.0%和58.0%;因此,它们被归类为轻微有害的杀虫剂(IOBC 2类)。双氟脲和苦参碱是无害的(IOBC 1级),寄生率分别降低了9.2%和27.6%。暴露于双三氟隆和苦参碱(分别为3.6和3.3天)以及吡丙芬和印楝素(分别为1.7和1.3天)的成年人的寿命显著低于对照组(4.67天)。在半田间试验中,根据处理后24小时的寄生蜂死亡率,大多数杀虫剂在番茄叶片上的残留被认为是中度有害到有害的,而在处理后7天和14天,它们是轻微有害的
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引用次数: 3
Survival and germinability of Rhynchosporium secalis conidia exposed to solar radiation 太阳辐射下毛缕草孢子的存活和萌发能力
Q3 Agricultural and Biological Sciences Pub Date : 2018-07-01 DOI: 10.2478/hppj-2018-0006
E. Al-shehadah, A. Al-Daoude, M. Jawhar
Abstract Rhynchosporium secalis, the causal agent of barley scald disease, is a fungus commonly found in the environment. Disease spread within a field and between fields occurs through the aerial dispersal of the fungal spores. However, not much is known about the survival potential of fungal conidia exposed to solar radiation. In the present study, detached conidia of R. secalis were exposed simultaneously in the field to direct sunlight or placed in an adjacent ventilated enclosure in the dark for periods ranging from 0.5 to 8h. In addition, conidia were either exposed or not exposed to UV-C light (254 nm) for periods ranging between 0.5 and 60 min in the laboratory. After exposure, conidia were placed on water agar Petri dishes and allowed to germinate for 24h. Germinability of conidia was reduced by up to 94% after 8h of exposure to solar irradiance (670-860 Wm-2) in the field in comparison to the non-exposed control. Germinability of conidia in the laboratory was reduced up to ~100% by doses of UV-C light of 3.2±0.7 Wm-2. The results of this study will contribute to a better understanding of the relationship between climatic conditions and barley scald epidemics
摘要大麦烫伤病的病原菌是一种常见于环境中的真菌。疾病在田地内和田地之间的传播是通过真菌孢子的空中传播发生的。然而,人们对暴露在太阳辐射下的真菌分生孢子的生存潜力知之甚少。在本研究中,将R.secalis分离的分生孢子同时暴露在阳光直射下,或在黑暗中放置在相邻通风的围栏中,时间从0.5到8小时不等。此外,分生孢子在实验室中暴露或不暴露于UV-C光(254nm)0.5至60分钟。暴露后,将分生孢子置于水琼脂培养皿上,并使其发芽24小时。与未暴露的对照相比,分生孢子在野外暴露于太阳辐照度(670-860 Wm-2)8小时后的发芽率降低了94%。3.2±0.7 Wm-2的UV-C光剂量可使分生孢子在实验室中的发芽率降低约100%。这项研究的结果将有助于更好地理解气候条件与大麦烫伤流行病之间的关系
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引用次数: 1
First record of parasitoids associated with insects inhabiting capsules of Papaver rhoeas in Greece 希腊首次发现寄生于罂粟花蒴果中的类寄生物
Q3 Agricultural and Biological Sciences Pub Date : 2018-07-01 DOI: 10.2478/hppj-2018-0007
F. Karamaouna, M. Samara, V. Kati, M. Mitroiu
Abstract A faunistic complex of chalcidoid parasitoids (Hymenoptera: Chalcidoidea) associated with cynipids and cecidomyids (Hymenoptera: Cynipidae; Diptera: Cecydomyiidae) inhabiting capsules of the annual weed Papaver rhoeas L. (corn poppy) was recorded in Amynteo, Northern Greece (2012) and Orchomenos, Voeotia, Central Greece (2013). The parasitoids are Idiomacromerus papaveris (Forster, 1856), Idiomacromerus sp., Pseudotorymus papaveris (Thomson, 1876) (Torymidae), Aprostocetus epicharmus Walker, 1839 (Eulophidae), and Cyrtoptyx sp. (Pteromalidae). Aprostocetus epicharmus was recorded only in Amynteo while Idiomacromerus spp. and Cyrtoptyx sp. only in Voeotia. This is the first record of these parasitoid species in corn poppy capsules in Greece. All parasitoids except the eulophid, which probably parasitizes Cecydomyiidae, are most likely parasitoids of Aylax papaveris (Perris, 1840) (Cynipidae)
摘要:在希腊北部的Amynteo(2012年)和希腊中部的Voeotia的Orchomenos(2013年)记录了一种与食蟹纲和盲肠蝇目(膜翅目:食蟹科;双翅目:盲肠蝇科)相关的小煤球类寄生蜂的区系复合体,它们栖息在一年生杂草Papover rhoeas L.(玉米罂粟)的囊中。拟寄生物有木瓜真核蜂(Forster,1856)、木瓜真核蝇属(Pseudotorymus papaveris,Thomson,1876)(Torymidae)、Aprostocetus epicharmus Walker,1839(Eulophidae)和Cyrtoptyx sp.(Pteromalidae)。Aprostocetus epicharmus仅在Amenteo中记录,而Idiomacromerus sp.和Cyrtotyx sp.仅在Voeotia中记录。这是希腊首次在玉米罂粟胶囊中记录到这些寄生蜂。除了可能寄生在Cecydomyiidae的eulophid外,所有的寄生蜂都很可能是Aylax papaveris的寄生蜂(Perris,1840)(Cynipidae)
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引用次数: 1
期刊
Hellenic Plant Protection Journal
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