Abstract Coptotriche spp. are known to cause damage on plants of Rosaceae and Fagaceae. Coptotriche angusticollella (Duponchel, 1843) (Lepidoptera: Tischeriidae) was recorded for the first time infesting the oil-bearing rose, Rosa damascena, at the last half of May 2017 in Isparta, Turkey. It should not be ignored that C. angusticollella can be a potential risk posed to the oil-bearing rose crop
{"title":"First record of Coptotriche angusticollella (Duponchel, 1843) (Lepidoptera: Tischeriidae) on the oil-bearing rose in Turkey","authors":"O. Demirözer, A. Uzun, S. Erbaş, F. Can","doi":"10.2478/hppj-2018-0008","DOIUrl":"https://doi.org/10.2478/hppj-2018-0008","url":null,"abstract":"Abstract Coptotriche spp. are known to cause damage on plants of Rosaceae and Fagaceae. Coptotriche angusticollella (Duponchel, 1843) (Lepidoptera: Tischeriidae) was recorded for the first time infesting the oil-bearing rose, Rosa damascena, at the last half of May 2017 in Isparta, Turkey. It should not be ignored that C. angusticollella can be a potential risk posed to the oil-bearing rose crop","PeriodicalId":39459,"journal":{"name":"Hellenic Plant Protection Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43430607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Al-Daoude, M. Jawhar, E. Al-shehadah, A. Shoaib, M. Orfi, M. Arabi
Abstract Net blotch (NB), caused by the necrotrophic fungal pathogen Pyrenophora teres f. teres, substantially reduces barley grain yield and quality worldwide. The role of salicylic acid (SA) signaling in NB resistance has been poorly documented. In this study, SA levels as well as the expression of the SA-responsive gene PR2 were monitored in infected leaves of two barley genotypes, Banteng (resistant) and WI2291 (susceptible), at different time points of infection. SA signaling was activated in bothgenotypes 24 hours post infection (hpi) as compared with non-inoculated plants. However, with or without pathogen pretreatment, SA signifi cantly increased (P=0.001) in Banteng comparing with WI2291. RT-PCR analysis revealed that PR2 expression increases in the resistant and susceptible genotypes over the inoculation time points, with maximum expression (6.4 and 1.99-fold, respectively) observed 6 dpi. PR2 expression was paralleled by an increase in leaf SA content as shown by the test coincidence (F3, 32 = 4.74, P = 0.001). Based on barley genotype resistance levels, our data strengthen the idea that SA signaling and PR2 play a role in barley NB reduction
{"title":"Changes in salicylic acid content and pathogenesis - related (PR2) gene expression during barley - Pyrenophora teres interaction","authors":"A. Al-Daoude, M. Jawhar, E. Al-shehadah, A. Shoaib, M. Orfi, M. Arabi","doi":"10.2478/hppj-2018-0010","DOIUrl":"https://doi.org/10.2478/hppj-2018-0010","url":null,"abstract":"Abstract Net blotch (NB), caused by the necrotrophic fungal pathogen Pyrenophora teres f. teres, substantially reduces barley grain yield and quality worldwide. The role of salicylic acid (SA) signaling in NB resistance has been poorly documented. In this study, SA levels as well as the expression of the SA-responsive gene PR2 were monitored in infected leaves of two barley genotypes, Banteng (resistant) and WI2291 (susceptible), at different time points of infection. SA signaling was activated in bothgenotypes 24 hours post infection (hpi) as compared with non-inoculated plants. However, with or without pathogen pretreatment, SA signifi cantly increased (P=0.001) in Banteng comparing with WI2291. RT-PCR analysis revealed that PR2 expression increases in the resistant and susceptible genotypes over the inoculation time points, with maximum expression (6.4 and 1.99-fold, respectively) observed 6 dpi. PR2 expression was paralleled by an increase in leaf SA content as shown by the test coincidence (F3, 32 = 4.74, P = 0.001). Based on barley genotype resistance levels, our data strengthen the idea that SA signaling and PR2 play a role in barley NB reduction","PeriodicalId":39459,"journal":{"name":"Hellenic Plant Protection Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44259281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Summary A straightforward LC-ESI-MS/MS method was developed and validated for the detection and quantitation of the herbicide glyphosate (GLY) and its metabolite aminomethyl phosphonic acid (AMPA) in honeybees. The method was validated, fulfilling the SANTE 11945/2015 guideline criteria, demonstrating acceptable mean recoveries at LOQ and 10×LOQ varying from 75-87% for both compounds. LOQ was determined at 0.2 and 0.5 μg/g bee body weight (bw) for GLY and AMPA respectively. Analysis of 14 honeybee samples displayed only one positive sample, containing GLY marginally above LOQ and traces of AMPA.
{"title":"Direct determination of glyphosate and aminomethyl phosphonic acid in honeybees","authors":"K. Kasiotis, Z.D. Tzouganaki, K. Machera","doi":"10.2478/hppj-2018-0005","DOIUrl":"https://doi.org/10.2478/hppj-2018-0005","url":null,"abstract":"Summary A straightforward LC-ESI-MS/MS method was developed and validated for the detection and quantitation of the herbicide glyphosate (GLY) and its metabolite aminomethyl phosphonic acid (AMPA) in honeybees. The method was validated, fulfilling the SANTE 11945/2015 guideline criteria, demonstrating acceptable mean recoveries at LOQ and 10×LOQ varying from 75-87% for both compounds. LOQ was determined at 0.2 and 0.5 μg/g bee body weight (bw) for GLY and AMPA respectively. Analysis of 14 honeybee samples displayed only one positive sample, containing GLY marginally above LOQ and traces of AMPA.","PeriodicalId":39459,"journal":{"name":"Hellenic Plant Protection Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.2478/hppj-2018-0005","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69211033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Summary Temperature, salinity and water deficit can be major environmental constraints which reduce distribution of indigenous plants in the Mediterranean region. Laboratory experiments were carried out to assess the effect of temperature, sodium chloride (NaCl) and polyethylene glycol 6000 (PEG) on germination of Ballota hirsuta seeds. The germination responses of seeds were determined over a wide range of temperature (5 to 35°C), salinity (0 to 136 mM NaCl) and water stress (0 to −1 MPa PEG). Germination percentage was optimum at 20°C (78%), and showed a decline at lower (5°C, 25%) or higher (30°C, 18%) temperature values and total inhibition of germination at 35°C. Under salinity and water stress conditions, there was a significant deterioration in most germination parameters such as lower final germination percentage, increased mean germination times and lower germination rates.
{"title":"Germination responses of Ballota hirsuta seeds under conditions of temperature, salinity and water stress","authors":"M. Dadach, Z. Mehdadi","doi":"10.2478/hppj-2018-0004","DOIUrl":"https://doi.org/10.2478/hppj-2018-0004","url":null,"abstract":"Summary Temperature, salinity and water deficit can be major environmental constraints which reduce distribution of indigenous plants in the Mediterranean region. Laboratory experiments were carried out to assess the effect of temperature, sodium chloride (NaCl) and polyethylene glycol 6000 (PEG) on germination of Ballota hirsuta seeds. The germination responses of seeds were determined over a wide range of temperature (5 to 35°C), salinity (0 to 136 mM NaCl) and water stress (0 to −1 MPa PEG). Germination percentage was optimum at 20°C (78%), and showed a decline at lower (5°C, 25%) or higher (30°C, 18%) temperature values and total inhibition of germination at 35°C. Under salinity and water stress conditions, there was a significant deterioration in most germination parameters such as lower final germination percentage, increased mean germination times and lower germination rates.","PeriodicalId":39459,"journal":{"name":"Hellenic Plant Protection Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.2478/hppj-2018-0004","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69210390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Summary The root-knot nematode Meloidogyne incognita is a major soil parasite of lentil crops. Increasing restrictions of chemical nematicides have triggered a growing attention and interest in alternate root-knot nematode management. The present study was conducted to examine the level of resistance and/or susceptibility of five lentil cultivars (PL-456, KLS-218, Desi, DPL-62, Malika), grown in pots, against the root-knot nematode M. incognita. Root-knot nematode reproduction and host damage were assessed by recording the nematode infestation levels and reduction percentage of plant growth parameters. Nematode response and plant growth differentiated amongst the lentil cultivars. None of the cultivars was found immune or highly resistant. The cultivar Malika was found moderately resistant as it showed the lowest number of galls and egg masses/root as well as the lowest reduction of plant fresh weight (10.4%) and dry weight (6.9%). On the other hand, the cultivar Desi manifested the highest susceptibility exhibiting the highest number of galls and egg masses. There was a significantly negative correlation between the number of galls and plant growth parameters (plant fresh and dry weight and plant height).
{"title":"Resistance screening of lentil cultivars against the root-knot nematode Meloidogyne incognita","authors":"T. Ansari, M. Asif, M. A. Siddiqui","doi":"10.2478/hppj-2018-0002","DOIUrl":"https://doi.org/10.2478/hppj-2018-0002","url":null,"abstract":"Summary The root-knot nematode Meloidogyne incognita is a major soil parasite of lentil crops. Increasing restrictions of chemical nematicides have triggered a growing attention and interest in alternate root-knot nematode management. The present study was conducted to examine the level of resistance and/or susceptibility of five lentil cultivars (PL-456, KLS-218, Desi, DPL-62, Malika), grown in pots, against the root-knot nematode M. incognita. Root-knot nematode reproduction and host damage were assessed by recording the nematode infestation levels and reduction percentage of plant growth parameters. Nematode response and plant growth differentiated amongst the lentil cultivars. None of the cultivars was found immune or highly resistant. The cultivar Malika was found moderately resistant as it showed the lowest number of galls and egg masses/root as well as the lowest reduction of plant fresh weight (10.4%) and dry weight (6.9%). On the other hand, the cultivar Desi manifested the highest susceptibility exhibiting the highest number of galls and egg masses. There was a significantly negative correlation between the number of galls and plant growth parameters (plant fresh and dry weight and plant height).","PeriodicalId":39459,"journal":{"name":"Hellenic Plant Protection Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69210249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. Yousefi, N. Hassanzadeh, K. Behboudi, F. B. Firouzjahi
Summary Endophytic bacteria of rice plants (Oryza sativa L.) from eight different cultivars were screened for their ability in inducing disease symptoms, plant growth promotion and antagonistic activity against Xanthomonas oryzae pv. oryzae. Out of the 63 whole isolates, five were plant pathogens. Based on phenotypic characteristics and 16S rDNA sequence analysis, these were identified as Pseudomonas oryzihabitans, P. fulva, Pantoea ananatis, Pantoea sp., Cellulomonas sp. Four out of the 63 isolates behaved as potentially good plant growth-promoting and biocontrol agents. These were identified as Bacillus sp., B. subtilis, Pseudomonas putida and Enterobacter sp. This is the first report of pathogenic and endophytic bacteria from rice grown in field conditions in North of Iran.
{"title":"Identification and determination of characteristics of endophytes from rice plants and their role in biocontrol of bacterial blight caused by Xanthomonas oryzae pv. oryzae","authors":"H. Yousefi, N. Hassanzadeh, K. Behboudi, F. B. Firouzjahi","doi":"10.2478/hppj-2018-0003","DOIUrl":"https://doi.org/10.2478/hppj-2018-0003","url":null,"abstract":"Summary Endophytic bacteria of rice plants (Oryza sativa L.) from eight different cultivars were screened for their ability in inducing disease symptoms, plant growth promotion and antagonistic activity against Xanthomonas oryzae pv. oryzae. Out of the 63 whole isolates, five were plant pathogens. Based on phenotypic characteristics and 16S rDNA sequence analysis, these were identified as Pseudomonas oryzihabitans, P. fulva, Pantoea ananatis, Pantoea sp., Cellulomonas sp. Four out of the 63 isolates behaved as potentially good plant growth-promoting and biocontrol agents. These were identified as Bacillus sp., B. subtilis, Pseudomonas putida and Enterobacter sp. This is the first report of pathogenic and endophytic bacteria from rice grown in field conditions in North of Iran.","PeriodicalId":39459,"journal":{"name":"Hellenic Plant Protection Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.2478/hppj-2018-0003","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69210339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Tavera, J. Lago, V.K.D. Magalong, G.A.V. Vidamo, J. Carandang, D. Amalin, J. Janairo
Summary Plants respond to stress or damage by releasing volatile compounds, primarily for defense purposes. These volatiles function as signals for different interactions of the plant with its environment. In this study, the volatile chemical profile of healthy Garcinia mangostana L. (mangosteen) leaves was compared against leaves infested with the scale insect, Aspidiotus rigidus Reyne (Hemiptera: Diaspididae) through solid phase microextraction (SPME) coupled with gas chromatography-mass spectrometry (GC-MS). Analyses revealed that leaves emit the terpene kaur-16-ene in response to A. rigidus infestation. Kaur-16-ene is a precursor of gibberellin, a plant hormone for growth and development. The results suggest that the emission of kaur-16-ene in infested G. mangostana may play a role relevant in increasing the resistance of the plant towards infestations by herbivores.
{"title":"Effect of Aspidiotus rigidus infestation on the volatile chemical profile of the host plant Garcinia mangostana","authors":"M. Tavera, J. Lago, V.K.D. Magalong, G.A.V. Vidamo, J. Carandang, D. Amalin, J. Janairo","doi":"10.2478/hppj-2018-0001","DOIUrl":"https://doi.org/10.2478/hppj-2018-0001","url":null,"abstract":"Summary Plants respond to stress or damage by releasing volatile compounds, primarily for defense purposes. These volatiles function as signals for different interactions of the plant with its environment. In this study, the volatile chemical profile of healthy Garcinia mangostana L. (mangosteen) leaves was compared against leaves infested with the scale insect, Aspidiotus rigidus Reyne (Hemiptera: Diaspididae) through solid phase microextraction (SPME) coupled with gas chromatography-mass spectrometry (GC-MS). Analyses revealed that leaves emit the terpene kaur-16-ene in response to A. rigidus infestation. Kaur-16-ene is a precursor of gibberellin, a plant hormone for growth and development. The results suggest that the emission of kaur-16-ene in infested G. mangostana may play a role relevant in increasing the resistance of the plant towards infestations by herbivores.","PeriodicalId":39459,"journal":{"name":"Hellenic Plant Protection Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69210248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Summary This is a detailed list of 223 species (727 specimens) of the family Scarabaeidae, the subfamilies Dynastinae (21 species), Melolonthinae (11 species), Rutelinae (29 species), Aphodiinae (104 species), Cetoniinae (59 species), represented in G.P. Moazzo’s collection at the Goulandris Natural History Museum. All label data for each specimen are given. The aim of this paper is to present this collection as reference data for comparison with recent entomological material.
{"title":"A catalogue of the Coleoptera of the G.P. Moazzo Collection in the Goulandris Natural History Museum. Part III (Scarabaeidae)","authors":"J. Tylianakis, M. Dimaki, V. Perdiou","doi":"10.1515/hppj-2017-0010","DOIUrl":"https://doi.org/10.1515/hppj-2017-0010","url":null,"abstract":"Summary This is a detailed list of 223 species (727 specimens) of the family Scarabaeidae, the subfamilies Dynastinae (21 species), Melolonthinae (11 species), Rutelinae (29 species), Aphodiinae (104 species), Cetoniinae (59 species), represented in G.P. Moazzo’s collection at the Goulandris Natural History Museum. All label data for each specimen are given. The aim of this paper is to present this collection as reference data for comparison with recent entomological material.","PeriodicalId":39459,"journal":{"name":"Hellenic Plant Protection Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41303414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Summary The invasive pink hibiscus mealybug, Maconellicoccus hirsutus (Green) (Hemiptera: Pseudococcidae), is reported for first time in Greece. Individuals of the mealybug were found infesting Hibiscus rosa-sinensis (Linnaeus) (Malvaceae) in private and public gardens in the urban environment in Rhodes, Dodecanese islands, East Greece. This is the first report of genus Maconellicoccus in Greece.
{"title":"The pink hibiscus mealybug Maconellicoccus hirsutus (Green) (Hemiptera: Pseudococcidae) in Greece","authors":"P. Milonas, G. Partsinevelos","doi":"10.1515/hppj-2017-0009","DOIUrl":"https://doi.org/10.1515/hppj-2017-0009","url":null,"abstract":"Summary The invasive pink hibiscus mealybug, Maconellicoccus hirsutus (Green) (Hemiptera: Pseudococcidae), is reported for first time in Greece. Individuals of the mealybug were found infesting Hibiscus rosa-sinensis (Linnaeus) (Malvaceae) in private and public gardens in the urban environment in Rhodes, Dodecanese islands, East Greece. This is the first report of genus Maconellicoccus in Greece.","PeriodicalId":39459,"journal":{"name":"Hellenic Plant Protection Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43180057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Summary The aphid Aphis craccivora was recorded on the crop of aronia, Aronia melanocarpa, in Montenegro, in June 2015 and 2016. This is the first record of A. craccivora in Montenegro on aronia.
{"title":"First record of Aphis craccivora Koch (Hemiptera: Aphididae) on aronia crop in Montenegro","authors":"N. Latinović, F. Karamaouna, N. Kavallieratos","doi":"10.1515/hppj-2017-0007","DOIUrl":"https://doi.org/10.1515/hppj-2017-0007","url":null,"abstract":"Summary The aphid Aphis craccivora was recorded on the crop of aronia, Aronia melanocarpa, in Montenegro, in June 2015 and 2016. This is the first record of A. craccivora in Montenegro on aronia.","PeriodicalId":39459,"journal":{"name":"Hellenic Plant Protection Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49422497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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